Sgr027990 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr027990
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	EF hand calcium-binding family protein
Location	tig00153056: 2207744 .. 2208220 (+)
RNA-Seq Expression	Sgr027990
Synteny	Sgr027990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTCAATTAGCGGGATCTCTGTCGGCCGAAACCGAGAGTCTCAGCCATATCTTCAGCCTAATAGAAGCATTCAGAGCGTTCGATGCAGACAACGACGGGCTGATCACGGCAGCAGAGGTTGGGGGGATTATGGGTTCGCTCGGGTACAACCTGAGCGAGCAAGACGTGAGCTTGATGATGGAGGAGGGAGACGTCGACAAAGACGGGTTGCTGAGTATAGAGGAGTTTCTGGAGATGAATGCAAAGAGTATGGAGGTGGGGGAGCTGGGGAGGTATCTGAAGATTGCCTTTGAGGCTCTCAAGGCCGATGGGGATGATCTGGTGAGCGGAGAGGAGCTGTATGACGTGTTTCTGAATGTGGGGTTGGAGGTTTCGCTTGAGGATTGCAATGCTATAGTGGCTTCCATGGATGGCGATGGGGATGGAGCTGTGTTTCTTGAAGATTTGAAACTTATTGTTAATTCGCTTCACTAA

mRNA sequence

ATGGCTCAATTAGCGGGATCTCTGTCGGCCGAAACCGAGAGTCTCAGCCATATCTTCAGCCTAATAGAAGCATTCAGAGCGTTCGATGCAGACAACGACGGGCTGATCACGGCAGCAGAGGTTGGGGGGATTATGGGTTCGCTCGGGTACAACCTGAGCGAGCAAGACGTGAGCTTGATGATGGAGGAGGGAGACGTCGACAAAGACGGGTTGCTGAGTATAGAGGAGTTTCTGGAGATGAATGCAAAGAGTATGGAGGTGGGGGAGCTGGGGAGGTATCTGAAGATTGCCTTTGAGGCTCTCAAGGCCGATGGGGATGATCTGGTGAGCGGAGAGGAGCTGTATGACGTGTTTCTGAATGTGGGGTTGGAGGTTTCGCTTGAGGATTGCAATGCTATAGTGGCTTCCATGGATGGCGATGGGGATGGAGCTGTGTTTCTTGAAGATTTGAAACTTATTGTTAATTCGCTTCACTAA

Coding sequence (CDS)

ATGGCTCAATTAGCGGGATCTCTGTCGGCCGAAACCGAGAGTCTCAGCCATATCTTCAGCCTAATAGAAGCATTCAGAGCGTTCGATGCAGACAACGACGGGCTGATCACGGCAGCAGAGGTTGGGGGGATTATGGGTTCGCTCGGGTACAACCTGAGCGAGCAAGACGTGAGCTTGATGATGGAGGAGGGAGACGTCGACAAAGACGGGTTGCTGAGTATAGAGGAGTTTCTGGAGATGAATGCAAAGAGTATGGAGGTGGGGGAGCTGGGGAGGTATCTGAAGATTGCCTTTGAGGCTCTCAAGGCCGATGGGGATGATCTGGTGAGCGGAGAGGAGCTGTATGACGTGTTTCTGAATGTGGGGTTGGAGGTTTCGCTTGAGGATTGCAATGCTATAGTGGCTTCCATGGATGGCGATGGGGATGGAGCTGTGTTTCTTGAAGATTTGAAACTTATTGTTAATTCGCTTCACTAA

Protein sequence

MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSLH

Homology

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BLAST of Sgr027990 vs. NCBI nr
Match: XP_022142920.1 (probable calcium-binding protein CML29 [Momordica charantia])

HSP 1 Score: 270.0 bits (689), Expect = 1.3e-68
Identity = 143/159 (89.94%), Postives = 153/159 (96.23%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ AGSLSAETESLSH+FSLIEAFRAFDADNDGLITA EVGGIMGSLGYNLSE+DV+LM
Sbjct: 1   MAQ-AGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGE-LGRYLKIAFEALKADGDDLVSGEELYDVFL 120
           MEEGDVDKDGLLS+ EFLEMNA+S+EVGE +G YLKIA+EALKADGDDLVSGEELYDVFL
Sbjct: 61  MEEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFL 120

Query: 121 NVGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSLH 159
           NVGLEVS+EDCNAIVASMDGD DGAVFLEDLKLIV+SLH
Sbjct: 121 NVGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 158

[Close]

BLAST of Sgr027990 vs. NCBI nr
Match: XP_038897482.1 (probable calcium-binding protein CML29 [Benincasa hispida])

HSP 1 Score: 270.0 bits (689), Expect = 1.3e-68
Identity = 139/158 (87.97%), Postives = 152/158 (96.20%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ AGSLSAETE+LSHIF+LIEAFRAFDADNDGLI++AEVGGIMGSLGY+++EQDV++M
Sbjct: 1   MAQ-AGSLSAETETLSHIFNLIEAFRAFDADNDGLISSAEVGGIMGSLGYDMNEQDVNMM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLN 120
           MEEGDVD+DGLLSI EFLEMNAK+MEVGELG YLKIAFEALKADGDDLVSGEELYDVF+N
Sbjct: 61  MEEGDVDRDGLLSIGEFLEMNAKNMEVGELGSYLKIAFEALKADGDDLVSGEELYDVFVN 120

Query: 121 VGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSLH 159
           +GLEVSLEDC AIVASMDGDGDGAVFL D KLIVNSLH
Sbjct: 121 LGLEVSLEDCMAIVASMDGDGDGAVFLHDFKLIVNSLH 157

[Close]

BLAST of Sgr027990 vs. NCBI nr
Match: XP_022981068.1 (probable calcium-binding protein CML29 [Cucurbita maxima])

HSP 1 Score: 263.5 bits (672), Expect = 1.2e-66
Identity = 137/157 (87.26%), Postives = 152/157 (96.82%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ A SLSAET+SLSHIFSLIEAFRAFDADNDGLI+AAEVGGIMGSLGYNLSEQDV++M
Sbjct: 1   MAQEA-SLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLN 120
           MEEGDVD+DGLLS+ EFLEM+A+S+EVGE+GRYLK+AFEAL ADGDDLVSGEELY+VF+N
Sbjct: 61  MEEGDVDRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSL 158
           +GL+VSLEDC AIVASMDGDGDGAVFLEDLKLIVNSL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Sgr027990 vs. NCBI nr
Match: XP_022941005.1 (probable calcium-binding protein CML29 [Cucurbita moschata])

HSP 1 Score: 263.1 bits (671), Expect = 1.6e-66
Identity = 133/151 (88.08%), Postives = 148/151 (98.01%), Query Frame = 0


Query: 7   SLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDV 66
           SLSAET+SLSHIFSLIEAFRAFDADNDGLI+AAEVGGIMGSLGYNLSEQDV++MMEEGDV
Sbjct: 6   SLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMMEEGDV 65

Query: 67  DKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVS 126
           D+DGLLS+ EFLEM+A+S+EVGE+GRYLK+AFEAL ADGDDLVSGEELY+VF+N+GL+VS
Sbjct: 66  DRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNLGLDVS 125

Query: 127 LEDCNAIVASMDGDGDGAVFLEDLKLIVNSL 158
           LEDC AIVASMDGDGDGAVFLEDLKLIVNSL
Sbjct: 126 LEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Sgr027990 vs. NCBI nr
Match: KAG6608067.1 (putative calcium-binding protein CML29, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031697.1 putative calcium-binding protein CML29, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.4 bits (664), Expect = 1.0e-65
Identity = 131/151 (86.75%), Postives = 147/151 (97.35%), Query Frame = 0


Query: 7   SLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDV 66
           SLSA+T+SLSHIFSLIEAFRAFDADNDGLI+AAEVGGIMGSLGYNLSEQDV++MMEEGDV
Sbjct: 6   SLSAQTDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNMMMEEGDV 65

Query: 67  DKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVS 126
           D+DGLLS+ EFLEM+ +S+EVGE+GRYLK+AFEAL ADGDDLVSGEELY+VF+N+GL+VS
Sbjct: 66  DQDGLLSMGEFLEMSGRSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNLGLDVS 125

Query: 127 LEDCNAIVASMDGDGDGAVFLEDLKLIVNSL 158
           LEDC AIVASMDGDGDGAVFLEDLKLIVNSL
Sbjct: 126 LEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

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BLAST of Sgr027990 vs. ExPASy Swiss-Prot
Match: Q5Z676 (Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 GN=CML29 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.0e-28
Identity = 73/155 (47.10%), Postives = 102/155 (65.81%), Query Frame = 0


Query: 5   AGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEG 64
           A  LS + E+LS+I SL+EAF+AFD+DNDGL+TA E+ G++ SLG +  E +V  M+   
Sbjct: 16  AQQLSVDFEALSYISSLVEAFQAFDSDNDGLVTAPELRGLLASLGLDKPEHEVRDMLARA 75

Query: 65  DVDKDGLLSIEEFLE-MNAKSMEVGELGRYLKIAFEALK-ADGDDLVSGEELYDVFLNVG 124
           D D+DG LS+EE L+ MNA  + +G LG  L+ A  AL+ A G D V G +     L+V 
Sbjct: 76  DADRDGKLSVEELLDVMNAGQLGLGALGALLQSAVPALESAAGPDGVLGADELARLLSVM 135

Query: 125 LEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSL 158
              S+EDC  I+A MDGDGDGA+ +E+ +L+   L
Sbjct: 136 GTASVEDCMEIIACMDGDGDGAISVEEFRLMAQLL 170

[Close]

BLAST of Sgr027990 vs. ExPASy Swiss-Prot
Match: Q9FYK2 (Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.8e-20
Identity = 49/139 (35.25%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 16  SHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIE 75
           + I  L   F+ FD + DG I++ E+G IM SLG+ + E+++   + E D   DG ++ E
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 76  EFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVA 135
           EF+E+N K M+  ++   LK AF     DG+  +S EEL++V  ++G E S+ +C  ++ 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 136 SMDGDGDGAVFLEDLKLIV 155
            +D DGDG +  E+ K+++
Sbjct: 153 GVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Sgr027990 vs. ExPASy Swiss-Prot
Match: P02595 (Calmodulin OS=Patinopecten sp. OX=6574 PE=1 SV=2)

HSP 1 Score: 91.7 bits (226), Expect = 8.2e-18
Identity = 50/134 (37.31%), Postives = 74/134 (55.22%), Query Frame = 0


Query: 23  EAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIEEFLEMNA 82
           EAF  FD D DG IT  E+G +M SLG N +E ++  M+ E D D DG +   EFL M A
Sbjct: 15  EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMA 74

Query: 83  KSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVASMDGDGD 142
           + M+  +    ++ AF     DGD  +S  EL  V  N+G +++ E+ + ++   D DGD
Sbjct: 75  RKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGD 134

Query: 143 GAVFLEDLKLIVNS 157
           G V  E+   ++ S
Sbjct: 135 GQVNYEEFVTMMTS 148

[Close]

BLAST of Sgr027990 vs. ExPASy Swiss-Prot
Match: P62184 (Calmodulin OS=Renilla reniformis OX=6136 PE=1 SV=2)

HSP 1 Score: 91.3 bits (225), Expect = 1.1e-17
Identity = 50/134 (37.31%), Postives = 74/134 (55.22%), Query Frame = 0


Query: 23  EAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIEEFLEMNA 82
           EAF  FD D DG IT  E+G +M SLG N +E ++  M+ E D D DG +   EFL M A
Sbjct: 15  EAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMA 74

Query: 83  KSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVASMDGDGD 142
           + M+  +    ++ AF     DGD  +S  EL  V  N+G +++ E+ + ++   D DGD
Sbjct: 75  RKMKDTDSEEEIREAFRVFDKDGDGFISAAELRHVMTNLGEKLTDEEVDEMIREADIDGD 134

Query: 143 GAVFLEDLKLIVNS 157
           G V  E+   ++ S
Sbjct: 135 GQVNYEEFVKMMTS 148

[Close]

BLAST of Sgr027990 vs. ExPASy Swiss-Prot
Match: P04352 (Calmodulin OS=Chlamydomonas reinhardtii OX=3055 PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 2.4e-17
Identity = 54/151 (35.76%), Postives = 83/151 (54.97%), Query Frame = 0


Query: 8   LSAETESLS--HIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGD 67
           ++A TE L+   I    EAF  FD D DG IT  E+G +M SLG N +E ++  M+ E D
Sbjct: 1   MAANTEQLTEEQIAEFKEAFALFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMISEVD 60

Query: 68  VDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEV 127
            D +G +   EFL + A+ M+  +    L+ AF+    DG+  +S  EL  V  N+G ++
Sbjct: 61  ADGNGTIDFPEFLMLMARKMKETDHEDELREAFKVFDKDGNGFISAAELRHVMTNLGEKL 120

Query: 128 SLEDCNAIVASMDGDGDGAVFLEDLKLIVNS 157
           S E+ + ++   D DGDG V  E+   ++ S
Sbjct: 121 SEEEVDEMIREADVDGDGQVNYEEFVRMMTS 151

[Close]

BLAST of Sgr027990 vs. ExPASy TrEMBL
Match: A0A6J1CMA3 (probable calcium-binding protein CML29 OS=Momordica charantia OX=3673 GN=LOC111012911 PE=4 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 6.2e-69
Identity = 143/159 (89.94%), Postives = 153/159 (96.23%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ AGSLSAETESLSH+FSLIEAFRAFDADNDGLITA EVGGIMGSLGYNLSE+DV+LM
Sbjct: 1   MAQ-AGSLSAETESLSHMFSLIEAFRAFDADNDGLITAPEVGGIMGSLGYNLSEEDVNLM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGE-LGRYLKIAFEALKADGDDLVSGEELYDVFL 120
           MEEGDVDKDGLLS+ EFLEMNA+S+EVGE +G YLKIA+EALKADGDDLVSGEELYDVFL
Sbjct: 61  MEEGDVDKDGLLSMGEFLEMNARSVEVGEVMGSYLKIAYEALKADGDDLVSGEELYDVFL 120

Query: 121 NVGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSLH 159
           NVGLEVS+EDCNAIVASMDGD DGAVFLEDLKLIV+SLH
Sbjct: 121 NVGLEVSVEDCNAIVASMDGDEDGAVFLEDLKLIVDSLH 158

[Close]

BLAST of Sgr027990 vs. ExPASy TrEMBL
Match: A0A6J1J135 (probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC111480326 PE=4 SV=1)

HSP 1 Score: 263.5 bits (672), Expect = 5.8e-67
Identity = 137/157 (87.26%), Postives = 152/157 (96.82%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ A SLSAET+SLSHIFSLIEAFRAFDADNDGLI+AAEVGGIMGSLGYNLSEQDV++M
Sbjct: 1   MAQEA-SLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLN 120
           MEEGDVD+DGLLS+ EFLEM+A+S+EVGE+GRYLK+AFEAL ADGDDLVSGEELY+VF+N
Sbjct: 61  MEEGDVDRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVN 120

Query: 121 VGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSL 158
           +GL+VSLEDC AIVASMDGDGDGAVFLEDLKLIVNSL
Sbjct: 121 LGLDVSLEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Sgr027990 vs. ExPASy TrEMBL
Match: A0A6J1FSB7 (probable calcium-binding protein CML29 OS=Cucurbita moschata OX=3662 GN=LOC111446418 PE=4 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 7.6e-67
Identity = 133/151 (88.08%), Postives = 148/151 (98.01%), Query Frame = 0


Query: 7   SLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDV 66
           SLSAET+SLSHIFSLIEAFRAFDADNDGLI+AAEVGGIMGSLGYNLSEQDV++MMEEGDV
Sbjct: 6   SLSAETDSLSHIFSLIEAFRAFDADNDGLISAAEVGGIMGSLGYNLSEQDVNVMMEEGDV 65

Query: 67  DKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVS 126
           D+DGLLS+ EFLEM+A+S+EVGE+GRYLK+AFEAL ADGDDLVSGEELY+VF+N+GL+VS
Sbjct: 66  DRDGLLSMGEFLEMSARSVEVGEVGRYLKVAFEALNADGDDLVSGEELYEVFVNLGLDVS 125

Query: 127 LEDCNAIVASMDGDGDGAVFLEDLKLIVNSL 158
           LEDC AIVASMDGDGDGAVFLEDLKLIVNSL
Sbjct: 126 LEDCMAIVASMDGDGDGAVFLEDLKLIVNSL 156

[Close]

BLAST of Sgr027990 vs. ExPASy TrEMBL
Match: A0A1S3B9P7 (probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.7e-64
Identity = 131/158 (82.91%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ AGSL  +TE+LSHIF+LIEAFRAFDADNDGLI++AEVGGI+GSLGYNLSE+DV +M
Sbjct: 1   MAQ-AGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLN 120
           MEEGD DKDGLLS+ EFLEMNAK+MEVGELG YLKIA EALKADGDDLVSGEELYDVF+N
Sbjct: 61  MEEGDADKDGLLSMGEFLEMNAKNMEVGELGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 VGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSLH 159
           +GL+VSLED  AIVAS+DGDGDGA+FL D KLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Sgr027990 vs. ExPASy TrEMBL
Match: A0A5D3D6S0 (Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1493G00180 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 2.7e-64
Identity = 131/158 (82.91%), Postives = 146/158 (92.41%), Query Frame = 0


Query: 1   MAQLAGSLSAETESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLM 60
           MAQ AGSL  +TE+LSHIF+LIEAFRAFDADNDGLI++AEVGGI+GSLGYNLSE+DV +M
Sbjct: 1   MAQ-AGSLLVDTEALSHIFNLIEAFRAFDADNDGLISSAEVGGIVGSLGYNLSEEDVKMM 60

Query: 61  MEEGDVDKDGLLSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLN 120
           MEEGD DKDGLLS+ EFLEMNAK+MEVGELG YLKIA EALKADGDDLVSGEELYDVF+N
Sbjct: 61  MEEGDADKDGLLSMGEFLEMNAKNMEVGELGSYLKIALEALKADGDDLVSGEELYDVFVN 120

Query: 121 VGLEVSLEDCNAIVASMDGDGDGAVFLEDLKLIVNSLH 159
           +GL+VSLED  AIVAS+DGDGDGA+FL D KLIVNSLH
Sbjct: 121 LGLDVSLEDSMAIVASLDGDGDGAMFLHDFKLIVNSLH 157

[Close]

BLAST of Sgr027990 vs. TAIR 10
Match: AT1G24620.1 (EF hand calcium-binding protein family )

HSP 1 Score: 100.5 bits (249), Expect = 1.3e-21
Identity = 49/139 (35.25%), Postives = 84/139 (60.43%), Query Frame = 0


Query: 16  SHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIE 75
           + I  L   F+ FD + DG I++ E+G IM SLG+ + E+++   + E D   DG ++ E
Sbjct: 33  TEIRELEAVFKKFDVNGDGKISSKELGAIMTSLGHEVPEEELEKAITEIDRKGDGYINFE 92

Query: 76  EFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVA 135
           EF+E+N K M+  ++   LK AF     DG+  +S EEL++V  ++G E S+ +C  ++ 
Sbjct: 93  EFVELNTKGMDQNDVLENLKDAFSVYDIDGNGSISAEELHEVLRSLGDECSIAECRKMIG 152

Query: 136 SMDGDGDGAVFLEDLKLIV 155
            +D DGDG +  E+ K+++
Sbjct: 153 GVDKDGDGTIDFEEFKIMM 171

[Close]

BLAST of Sgr027990 vs. TAIR 10
Match: AT1G18210.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 88.2 bits (217), Expect = 6.5e-18
Identity = 44/145 (30.34%), Postives = 84/145 (57.93%), Query Frame = 0


Query: 12  TESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGL 71
           T  +++   L + F  FD++ DG I+  E+GG+  ++G + +E +++ ++EE D D+DG 
Sbjct: 15  TVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGY 74

Query: 72  LSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCN 131
           ++++EF  +   S    E    ++ AF+    D + L+S  EL+ V   +G+  S+EDC 
Sbjct: 75  INLDEFSTLCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 134

Query: 132 AIVASMDGDGDGAVFLEDLKLIVNS 157
            ++  +D DGDG V  E+ + ++ S
Sbjct: 135 RMIGPVDADGDGNVNFEEFQKMMTS 155

[Close]

BLAST of Sgr027990 vs. TAIR 10
Match: AT1G18210.2 (Calcium-binding EF-hand family protein )

HSP 1 Score: 88.2 bits (217), Expect = 6.5e-18
Identity = 44/145 (30.34%), Postives = 84/145 (57.93%), Query Frame = 0


Query: 12  TESLSHIFSLIEAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGL 71
           T  +++   L + F  FD++ DG I+  E+GG+  ++G + +E +++ ++EE D D+DG 
Sbjct: 15  TVDMANPEELKKVFDQFDSNGDGKISVLELGGVFKAMGTSYTETELNRVLEEVDTDRDGY 74

Query: 72  LSIEEFLEMNAKSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCN 131
           ++++EF  +   S    E    ++ AF+    D + L+S  EL+ V   +G+  S+EDC 
Sbjct: 75  INLDEFSTLCRSSSSAAE----IRDAFDLYDQDKNGLISASELHQVLNRLGMSCSVEDCT 134

Query: 132 AIVASMDGDGDGAVFLEDLKLIVNS 157
            ++  +D DGDG V  E+ + ++ S
Sbjct: 135 RMIGPVDADGDGNVNFEEFQKMMTS 155

[Close]

BLAST of Sgr027990 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 85.5 bits (210), Expect = 4.2e-17
Identity = 48/132 (36.36%), Postives = 71/132 (53.79%), Query Frame = 0


Query: 23  EAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIEEFLEMNA 82
           EAF  FD D DG IT  E+G +M SLG N +E ++  M+ E D D +G +   EFL + A
Sbjct: 15  EAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA 74

Query: 83  KSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVASMDGDGD 142
           K M+  +    LK AF     D +  +S  EL  V  N+G +++ E+   ++   D DGD
Sbjct: 75  KKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDEEVEEMIREADVDGD 134

Query: 143 GAVFLEDLKLIV 155
           G +  E+   I+
Sbjct: 135 GQINYEEFVKIM 146

[Close]

BLAST of Sgr027990 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 85.5 bits (210), Expect = 4.2e-17
Identity = 47/127 (37.01%), Postives = 70/127 (55.12%), Query Frame = 0


Query: 23  EAFRAFDADNDGLITAAEVGGIMGSLGYNLSEQDVSLMMEEGDVDKDGLLSIEEFLEMNA 82
           EAF  FD D DG IT  E+G +M SLG N +E ++  M+ E D D +G +   EFL + A
Sbjct: 15  EAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLNLMA 74

Query: 83  KSMEVGELGRYLKIAFEALKADGDDLVSGEELYDVFLNVGLEVSLEDCNAIVASMDGDGD 142
           + M+  +    LK AF     D +  +S  EL  V  N+G ++S E+ + ++   D DGD
Sbjct: 75  RKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDEEVDEMIREADVDGD 134

Query: 143 GAVFLED 150
           G +  E+
Sbjct: 135 GQINYEE 141

The following BLAST results are available for this feature:

BLAST of Sgr027990 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022142920.1	1.3e-68	89.94	probable calcium-binding protein CML29 [Momordica charantia]	[more]
XP_038897482.1	1.3e-68	87.97	probable calcium-binding protein CML29 [Benincasa hispida]	[more]
XP_022981068.1	1.2e-66	87.26	probable calcium-binding protein CML29 [Cucurbita maxima]	[more]
XP_022941005.1	1.6e-66	88.08	probable calcium-binding protein CML29 [Cucurbita moschata]	[more]
KAG6608067.1	1.0e-65	86.75	putative calcium-binding protein CML29, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Sgr027990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5Z676	3.0e-28	47.10	Probable calcium-binding protein CML29 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9FYK2	1.8e-20	35.25	Probable calcium-binding protein CML25 OS=Arabidopsis thaliana OX=3702 GN=CML25 ...	[more]
P02595	8.2e-18	37.31	Calmodulin OS=Patinopecten sp. OX=6574 PE=1 SV=2	[more]
P62184	1.1e-17	37.31	Calmodulin OS=Renilla reniformis OX=6136 PE=1 SV=2	[more]
P04352	2.4e-17	35.76	Calmodulin OS=Chlamydomonas reinhardtii OX=3055 PE=1 SV=2	[more]

BLAST of Sgr027990 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CMA3	6.2e-69	89.94	probable calcium-binding protein CML29 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1J135	5.8e-67	87.26	probable calcium-binding protein CML29 OS=Cucurbita maxima OX=3661 GN=LOC1114803...	[more]
A0A6J1FSB7	7.6e-67	88.08	probable calcium-binding protein CML29 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A1S3B9P7	2.7e-64	82.91	probable calcium-binding protein CML29 OS=Cucumis melo OX=3656 GN=LOC103487564 P...	[more]
A0A5D3D6S0	2.7e-64	82.91	Putative calcium-binding protein CML29 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Sgr027990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G24620.1	1.3e-21	35.25	EF hand calcium-binding protein family	[more]
AT1G18210.1	6.5e-18	30.34	Calcium-binding EF-hand family protein	[more]
AT1G18210.2	6.5e-18	30.34	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	4.2e-17	36.36	calmodulin 4	[more]
AT5G21274.1	4.2e-17	37.01	calmodulin 6	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 20..48 e-value: 0.0043 score: 26.3 coord: 129..157 e-value: 30.0 score: 5.8 coord: 56..84 e-value: 1.5 score: 16.3
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 21..80 e-value: 1.6E-10 score: 41.3
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 125..158 score: 9.241175
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 52..87 score: 9.492233
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 16..51 score: 12.672309
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 21..80 e-value: 2.70393E-16 score: 66.8025
None	No IPR available	GENE3D	1.10.238.10		coord: 14..157 e-value: 5.8E-30 score: 106.1
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 13..156
None	No IPR available	PANTHER	PTHR23050:SF382	CALCIUM-BINDING PROTEIN CML25-RELATED	coord: 13..156
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 29..41
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 65..77
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 16..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr027990.1	Sgr027990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding