Sgr027960 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr027960 vs. NCBI nr
Match: XP_022139412.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Momordica charantia])

HSP 1 Score: 980.3 bits (2533), Expect = 1.1e-281
Identity = 475/575 (82.61%), Postives = 519/575 (90.26%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP +K KQQ+Q LP KVDIL+NLKN+C+KLLD FVDSVFEFSDQTLHPTQGNLAPVEE
Sbjct: 18  PMIPMRKQKQQKQLLPLKVDILKNLKNSCIKLLDVFVDSVFEFSDQTLHPTQGNLAPVEE 77

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +G AVAVT I+GT+P+EFP+GIYIRNGPNP+FGGLKST S+FGRS+Y WVEGEGMLHALY
Sbjct: 78  IGTAVAVTTIQGTIPQEFPQGIYIRNGPNPLFGGLKSTKSIFGRSNYTWVEGEGMLHALY 137

Query: 344 FNKD--SRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFG 403
           FNKD     KWNLFYNNRYVQT+TF+LEK VKN P FLPA+EGDSLAVLSAFFLN+LRFG
Sbjct: 138 FNKDHTKLRKWNLFYNNRYVQTQTFQLEKHVKNGPYFLPAIEGDSLAVLSAFFLNMLRFG 197

Query: 404 KVNKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPE 463
            VNKD+SNTNV EHSGKF S+A+NS+ Q+IDIM+L++LG+WDVNGAWNRPFTSHPKKAP 
Sbjct: 198 TVNKDMSNTNVIEHSGKFYSVADNSLPQQIDIMSLQSLGVWDVNGAWNRPFTSHPKKAPG 257

Query: 464 TDELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVT 523
           T ELV+MGVTP KPFM++GIISEDGK MVHKVDVKLSR S SHEIGVTRRYNVILD+PVT
Sbjct: 258 TGELVIMGVTPAKPFMLLGIISEDGKEMVHKVDVKLSRGSFSHEIGVTRRYNVILDYPVT 317

Query: 524 IDLNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVV 583
           ID NRLI GGSLIKYDKEGYA+IGVMPRYGD DSIQWFQVKPNCTMHLFN FE  +EVVV
Sbjct: 318 IDFNRLITGGSLIKYDKEGYARIGVMPRYGDGDSIQWFQVKPNCTMHLFNSFEQNDEVVV 377

Query: 584 WGCRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNT-DSSIEDGSLFSRAYEWRLN 643
           WGCRASDSVIPGPEKG NKFEWFS RFKPLP  D + +++  SS+EDGSLFSRAYEWRLN
Sbjct: 378 WGCRASDSVIPGPEKGHNKFEWFSNRFKPLPIADEQNSDSCSSSMEDGSLFSRAYEWRLN 437

Query: 644 LKTGEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLH 703
           LKTGEARER L+GTQ SMDFPFINSRFTGL+NKFGYAQ+LDSFASSNSGMFKFGGLAKLH
Sbjct: 438 LKTGEARERFLSGTQFSMDFPFINSRFTGLQNKFGYAQILDSFASSNSGMFKFGGLAKLH 497

Query: 704 FEEP-QSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHN 763
           FEEP QS  FSLPKK   +  IKVEYHMFEN+SFCSGASFV RE   EEDDGWIIAH+HN
Sbjct: 498 FEEPDQSCGFSLPKKWGNE-AIKVEYHMFENSSFCSGASFVPREDGAEEDDGWIIAHLHN 557

Query: 764 EITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           EITNTSQVYIIDAKKFSEEAVAKITLP RVPYGFH
Sbjct: 558 EITNTSQVYIIDAKKFSEEAVAKITLPQRVPYGFH 591

BLAST of Sgr027960 vs. NCBI nr
Match: XP_022980961.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 939.9 bits (2428), Expect = 1.7e-269
Identity = 453/571 (79.33%), Postives = 511/571 (89.49%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ+Q +  +VDI++ LKN CMKL+DGFVD VFEFSDQTLHP Q NLAPVEE
Sbjct: 37  PLIPGKKLKQQQQHV--RVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEE 96

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAVT I+GT+P++FP+GI++RNGPNP+FGGLKST SMFGRSS+IWVEGEGMLHA+Y
Sbjct: 97  IGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMY 156

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K+  GKW+L YNNRYVQTE+FR EK VKNRPSFLPAVEGD++A+++AF LN LRFG+V
Sbjct: 157 FKKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEV 216

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSGKF S+AENSM Q+IDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 217 NKDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 276

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIGVTRR+NVILD+PVTID
Sbjct: 277 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTID 336

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
           LNRLIRGGSLIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMHLFNCFE + EVVVWG
Sbjct: 337 LNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWG 396

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS+IPGPEKGLNKFEWFS+RFKP+  TD  E NT    EDGSL S AYEWRLNLKT
Sbjct: 397 CRATDSIIPGPEKGLNKFEWFSQRFKPVHVTD--EQNT----EDGSLLSCAYEWRLNLKT 456

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GEARER LTG Q SMDFPFINS FTGLKNK+GYAQVLDS ASSNSG+FKFGGLAKLHFEE
Sbjct: 457 GEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEE 516

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGWIIAHVHNEIT 
Sbjct: 517 PQSGEVSLLKDSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITK 576

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 577 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 598

BLAST of Sgr027960 vs. NCBI nr
Match: XP_022940198.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita moschata] >XP_022940199.1 carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita moschata])

HSP 1 Score: 931.4 bits (2406), Expect = 6.1e-267
Identity = 446/571 (78.11%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ      +VDI+Q LKN CMKL+D FVD+VFEFSDQTLHP Q NLAPVEE
Sbjct: 32  PLIPGKKLKQQH----VRVDIVQKLKNGCMKLVDAFVDTVFEFSDQTLHPNQSNLAPVEE 91

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAV  I+GT+P++FP+GI++RNGPNP+FGGLK T SM GRSS+IWVEGEGMLHA+Y
Sbjct: 92  IGAPVAVADIQGTLPQQFPQGIFLRNGPNPLFGGLKWTRSMLGRSSFIWVEGEGMLHAIY 151

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K+  G+W+L+YNNRYVQT++F+ EK VKNRPSFLPAVEGD+LAV++AF LN LRFG+V
Sbjct: 152 FKKNINGEWHLYYNNRYVQTDSFQFEKHVKNRPSFLPAVEGDALAVVAAFVLNWLRFGEV 211

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSG+F S+AENSM QEIDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 212 NKDMSNTNVIEHSGRFYSVAENSMPQEIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 271

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIGVTRR+NVILD+PVTID
Sbjct: 272 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTID 331

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
           LNRLIRGGSLIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMHLFNCFE + EVVVWG
Sbjct: 332 LNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWG 391

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS+IPGPEKGLNKFEWFS+RFKP+  TD      + + EDGSL S AYEWRLNLKT
Sbjct: 392 CRATDSIIPGPEKGLNKFEWFSQRFKPVHVTD------EQNAEDGSLLSCAYEWRLNLKT 451

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GEARER LTG Q SMDFPFINS FTGLKN++GYAQVLDS ASSNSGMFKFGGLAKLHFEE
Sbjct: 452 GEARERFLTGNQFSMDFPFINSAFTGLKNRYGYAQVLDSVASSNSGMFKFGGLAKLHFEE 511

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGWIIAHVHNEITN
Sbjct: 512 PQSGEVSLLKGSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITN 571

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 572 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 591

BLAST of Sgr027960 vs. NCBI nr
Match: XP_023523500.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 929.9 bits (2402), Expect = 1.8e-266
Identity = 446/571 (78.11%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ      +VDI++ LKN CMKL+D FVD+VFEFSDQT+HP Q NL+PVEE
Sbjct: 2   PLIPGKKLKQQ----DVRVDIVKKLKNGCMKLVDAFVDTVFEFSDQTVHPNQTNLSPVEE 61

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAV  I+GT+P++FP+GI++RNGPNP+FGGLKST SMFGRSS+IWVEGEGMLHA+Y
Sbjct: 62  IGAPVAVADIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAIY 121

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K   G+W+L YNNRYVQTE+FR EK VKNRPSFLPAVEGD++AV++AF LN LRFG+V
Sbjct: 122 FKKHINGEWHLLYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAVVAAFVLNWLRFGEV 181

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSG+F S+AENSM QEIDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 182 NKDMSNTNVIEHSGRFYSVAENSMPQEIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 241

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIG+TRR+NVILD+PVTID
Sbjct: 242 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGITRRFNVILDYPVTID 301

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
           LNRLIRGGSLIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMH+FNCFE E EVVVWG
Sbjct: 302 LNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHVFNCFEHEHEVVVWG 361

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS IPGPEKGLNKFEWFS+RFKP+  TD  E NT    EDGSL SRAY+WRLNLKT
Sbjct: 362 CRATDSFIPGPEKGLNKFEWFSQRFKPVCVTD--EQNT----EDGSLLSRAYQWRLNLKT 421

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GE RER LTG Q SMDFPFINS FTGLKNK+GYAQVLDS ASSNSG+FKFGGLAKLHFEE
Sbjct: 422 GEVRERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEE 481

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGW+IAHVHNEITN
Sbjct: 482 PQSGEVSLLKDSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWVIAHVHNEITN 541

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 542 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 561

BLAST of Sgr027960 vs. NCBI nr
Match: XP_022980963.1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 888.6 bits (2295), Expect = 4.5e-254
Identity = 434/571 (76.01%), Postives = 490/571 (85.81%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ+Q +  +VDI++ LKN CMKL+DGFVD VFEFSDQTLHP Q NLAPVEE
Sbjct: 37  PLIPGKKLKQQQQHV--RVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEE 96

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAVT I+GT+P++FP+GI++RNGPNP+FGGLKST SMFGRSS+IWVEGEGMLHA+Y
Sbjct: 97  IGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMY 156

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K+  GKW+L YNNRYVQTE+FR EK VKNRPSFLPAVEGD++A+++AF LN LRFG+V
Sbjct: 157 FKKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEV 216

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSGKF S+AENSM Q+IDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 217 NKDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 276

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIGVTRR            
Sbjct: 277 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRR------------ 336

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
                    LIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMHLFNCFE + EVVVWG
Sbjct: 337 ---------LIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWG 396

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS+IPGPEKGLNKFEWFS+RFKP+  TD  E NT    EDGSL S AYEWRLNLKT
Sbjct: 397 CRATDSIIPGPEKGLNKFEWFSQRFKPVHVTD--EQNT----EDGSLLSCAYEWRLNLKT 456

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GEARER LTG Q SMDFPFINS FTGLKNK+GYAQVLDS ASSNSG+FKFGGLAKLHFEE
Sbjct: 457 GEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEE 516

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGWIIAHVHNEIT 
Sbjct: 517 PQSGEVSLLKDSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITK 576

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 577 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 577

BLAST of Sgr027960 vs. ExPASy Swiss-Prot
Match: C3VEQ4 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Oncidium hybrid cultivar OX=141207 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 231.9 bits (590), Expect = 3.0e-59
Identity = 185/599 (30.88%), Postives = 281/599 (46.91%), Query Frame = 0

Query: 230 KPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEEMGAAVA 289
           +PK ++  L S +D+++       K++  F       S + LH   GN APV +     A
Sbjct: 36  QPKPRKGILSSTIDLIE-------KVIVYFAHD----SSKPLHYLTGNFAPVRDETPPFA 95

Query: 290 VTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALYFNKDSR 349
              + G++P     G ++R GPNP F  +         + Y W +G+GM+H L   KD +
Sbjct: 96  ALTVHGSLPVCL-NGEFVRVGPNPKFSPV---------AGYHWFDGDGMIHGLRI-KDGK 155

Query: 350 GKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKVNKDLS- 409
                 Y +RYV+T     E+  +    F+   +   L  L    + LLR      D+S 
Sbjct: 156 AT----YVSRYVKTSKLMQEEYFEG-AKFMKIGDLKGLFGLFMVQMELLRAKLKILDVSY 215

Query: 410 -----NTNVFEHSGKFLSLAENSMAQEIDIM---TLETLGIWDVNGAWNRPFTSHPKKAP 469
                NT +  H GK L+L+E      I ++    L+TLG+ D N      FT+HPK  P
Sbjct: 216 GRSTANTAMIYHHGKLLALSEGDKPYVIKVLEDGDLQTLGLLDYNKRLAHSFTAHPKVDP 275

Query: 470 ETDELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPV 529
            TDE+   G + T P++   +I++DG  M++ V + +    + H+  +T  Y + LD P+
Sbjct: 276 FTDEMFTFGYSHTSPYITYRVITKDGV-MLNPVPITIPEPIMMHDFAITENYAIFLDLPL 335

Query: 530 TIDLNRLIRGGSLIKYDKEGYAKIGVMPRYG-DADSIQWFQVKPNC-TMHLFNCFEDEEE 589
                  I+G  +  +D    AK GV+PRY  D   I+WF + PNC   H  N +ED +E
Sbjct: 336 YFRPKETIKGKLIFTFDPTKKAKFGVLPRYAKDEQLIRWFDL-PNCFIFHNANAWEDGDE 395

Query: 590 VVVWGCRASD---SVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAY 649
           VV+  CR  +    ++ G  K   K E FS                          ++ Y
Sbjct: 396 VVLITCRLKNIDLDLVNGAVK--EKLENFS--------------------------NQLY 455

Query: 650 EWRLNLKTGEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGG 709
           E R N+KTG A ++ L+   +  DFP IN  +TG K ++ Y     SFA+ +    K  G
Sbjct: 456 EMRFNMKTGAATQKELSAPMV--DFPRINENYTGRKQRYVYC---TSFANID----KVNG 515

Query: 710 LAKLHFE-EPQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSP--EEDDGW 769
           + K     EP++      KK  E     +         F S A FV RE     EEDDG+
Sbjct: 516 IVKFDLHAEPET-----GKKVLEVGGNVLGIFDLGPGRFGSEAVFVPREAGTRVEEDDGY 559

Query: 770 IIAHVHNEITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFHESDCMFTVSDVELEPEE 812
           +I  V++E T  S+VY+IDAK  S E VA + LP RVPYGFH     F V++ +++ ++
Sbjct: 576 LIFFVYDETTGESKVYVIDAKTMSPEPVAVVDLPCRVPYGFH----AFFVNEEQIQKQQ 559

BLAST of Sgr027960 vs. ExPASy Swiss-Prot
Match: Q84KG5 (Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus OX=82528 GN=CCD PE=1 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 5.1e-59
Identity = 176/577 (30.50%), Postives = 277/577 (48.01%), Query Frame = 0

Query: 246 QNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGI 305
           + L ++ + L++  V  +F    +  H   GN APV +         + G +PE    G 
Sbjct: 24  KGLVSSAVDLIEKAVVYLFHDKSKPCHYLSGNFAPVVDETPPCPDLPVRGHLPECL-NGE 83

Query: 306 YIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTET 365
           ++R GPNP F  +         + Y W +G+GM+H +   KD +      Y +RYV+T  
Sbjct: 84  FVRVGPNPKFMPV---------AGYHWFDGDGMIHGMRI-KDGKAT----YASRYVKTSR 143

Query: 366 FRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKVNKDLS------NTNVFEHSGKF 425
            + E+  +  P F+   +      L    + LLR      D+S      NT +  H GK 
Sbjct: 144 LKQEEYFEG-PKFMKIGDLKGFFGLFMVQMQLLRAKLKVIDVSYGVGTGNTALIYHHGKL 203

Query: 426 LSLAENSMAQEIDIM---TLETLGIWDVNGAWNRPFTSHPKKAPETDELVVMGVTPTKPF 485
           L+L+E      + ++    L+TLG+ D +   +  FT+HPK  P TDE+   G   T P+
Sbjct: 204 LALSEADKPYVVKVLEDGDLQTLGLLDYDKRLSHSFTAHPKVDPFTDEMFTFGYAHTPPY 263

Query: 486 MVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTIDLNRLIRGGSLI-K 545
           +   +IS+DG  M   V + +  S + H+  +T  Y++ +D P+      +++GG LI  
Sbjct: 264 VTYRVISKDGV-MRDPVPITIPASVMMHDFAITENYSIFMDLPLYFQPKEMVKGGKLIFS 323

Query: 546 YDKEGYAKIGVMPRYGDADS-IQWFQVKPNC-TMHLFNCFEDEEEVVVWGCRASDSVIPG 605
           +D    A+ GV+PRY   DS I+WF++ PNC   H  N +E+ +EVV+  CR  +     
Sbjct: 324 FDATKKARFGVLPRYAKDDSLIRWFEL-PNCFIFHNANAWEEGDEVVLITCRLEN----- 383

Query: 606 PEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKTGEARERSLTG 665
                             P  D         +E  +  +  YE R N+KTG A ++ L+ 
Sbjct: 384 ------------------PDLDMVNGAVKEKLE--NFKNELYEMRFNMKTGAASQKQLSV 443

Query: 666 TQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPK 725
           +  ++DFP IN  +T  K ++ Y  +LD+  +   G+ KF     LH  EP++ +  L  
Sbjct: 444 S--AVDFPRINESYTTRKQRYVYGTILDNI-TKVKGIIKF----DLH-AEPEAGKKKLEV 503

Query: 726 KCEEQRIIKVEYHMFENNSFCSGASFVSREG--SPEEDDGWIIAHVHNEITNTSQVYIID 785
               Q I  +         + S A FV RE     EEDDG++I  VH+E T  S+V +ID
Sbjct: 504 GGNVQGIFDL-----GPGRYGSEAVFVPRERGIKSEEDDGYLIFFVHDENTGKSEVNVID 540

Query: 786 AKKFSEEAVAKITLPHRVPYGFHESDCMFTVSDVELE 809
           AK  S E VA + LP+RVPYGFH     F V++ +L+
Sbjct: 564 AKTMSAEPVAVVELPNRVPYGFH----AFFVNEEQLQ 540

BLAST of Sgr027960 vs. ExPASy Swiss-Prot
Match: Q8LP17 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum OX=3888 GN=CCD1 PE=2 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 8.8e-59
Identity = 178/573 (31.06%), Postives = 276/573 (48.17%), Query Frame = 0

Query: 255 LLDGFVDSVFEFSDQTLHPTQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPNPV 314
           LL+  +  +F  S    H   GN APV++    V    ++G +P+    G ++R GPNP 
Sbjct: 29  LLEKIIVKLFYDSSLPHHWLSGNFAPVKDETPPVKDLTVQGHLPDCL-NGEFVRVGPNPK 88

Query: 315 FGGLKSTHSMFGRSSYIWVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTETFRLEKLVKN 374
           F  +         + Y W +G+GM+H L   KD +      Y +R+V+T  F+ E+   N
Sbjct: 89  FSPV---------AGYHWFDGDGMIHGLRI-KDGKAS----YVSRFVKTSRFKQEEYF-N 148

Query: 375 RPSFLPAVEGDSLAVLSAFFLNLLRFGKVNKDLS------NTNVFEHSGKFLSLAENSMA 434
              F+   +   L  L    + +LR      D+S      NT +  H  K L+L+E    
Sbjct: 149 GSKFMKIGDLKGLFGLLMVNMQMLRAKLKILDVSYGHGTANTALVYHHQKLLALSEGDKP 208

Query: 435 QEIDIM---TLETLGIWDVNGAWNRPFTSHPKKAPETDELVVMGVTPTKPFMVVGIISED 494
             I +     L+TLG+ D +      FT+HPK  P T E+   G + T P++   +IS+D
Sbjct: 209 YAIKVFEDGDLQTLGMLDYDKRLGHNFTAHPKVDPFTGEMFTFGYSHTAPYVTYRVISKD 268

Query: 495 GKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTIDLNRLIRGGSLI-KYDKEGYAKI 554
           G  M   V + +S   + H+  +T  Y++ +D P+      +++  +LI  +D    A+ 
Sbjct: 269 G-FMQDPVPITISDPVMMHDFAITENYSIFMDLPLYFRPKEMVKNKTLIFSFDSTKKARF 328

Query: 555 GVMPRYGDADS-IQWFQVKPNC-TMHLFNCFEDEEEVVVWGCRASD---SVIPGPEKGLN 614
           GV+PRY   D  I+WF++ PNC   H  N +E+E+E+V+  CR  +    V+ G  K   
Sbjct: 329 GVLPRYAKDDKHIRWFEL-PNCFIFHNANAWEEEDEIVLITCRLENPNLDVVGGAVK--E 388

Query: 615 KFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKTGEARERSLTGTQISMD 674
           K + FS                          +  YE R N+KTGEA ++ L+ +  ++D
Sbjct: 389 KLDNFS--------------------------NELYEMRFNMKTGEASQKKLSAS--TVD 448

Query: 675 FPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPKKCEEQR 734
           FP +N  +TG K ++ Y   LDS A   +G+ KF     LH  EP S +  L      Q 
Sbjct: 449 FPRVNESYTGRKQRYVYGTTLDSIAKV-TGIIKF----DLH-AEPDSGKTKLEVGGNVQG 508

Query: 735 IIKVEYHMFENNSFCSGASFVSR--EGSPEEDDGWIIAHVHNEITNTSQVYIIDAKKFSE 794
           +     +      F S A +V R      EEDDG++I  VH+E T  S V++IDAK+ S 
Sbjct: 509 L-----YDLGPGRFGSEAVYVPRVPGTDSEEDDGYLIFFVHDENTGKSFVHVIDAKRMSA 538

Query: 795 EAVAKITLPHRVPYGFHESDCMFTVSDVELEPE 811
           E VA + LP RVPYGFH     F V++ +L+ +
Sbjct: 569 EPVAVVELPQRVPYGFH----AFFVTEDQLQEQ 538

BLAST of Sgr027960 vs. ExPASy Swiss-Prot
Match: O65572 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=CCD1 PE=1 SV=2)

HSP 1 Score: 228.0 bits (580), Expect = 4.4e-58
Identity = 175/560 (31.25%), Postives = 264/560 (47.14%), Query Frame = 0

Query: 253 MKLLDGFVDSVFEFSDQTLHPTQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPN 312
           + LL+  V  +   +   LH   GN AP+ +    V    + G +PE    G ++R GPN
Sbjct: 27  LDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFLPECL-NGEFVRVGPN 86

Query: 313 PVFGGLKSTHSMFGRSSYIWVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTETFRLEKLV 372
           P F  +         + Y W +G+GM+H +   KD +      Y +RYV+T   + E+  
Sbjct: 87  PKFDAV---------AGYHWFDGDGMIHGVRI-KDGKAT----YVSRYVKTSRLKQEE-- 146

Query: 373 KNRPSFLPAVEGDSLAVLSAFF----LNLLRFGKVNKDL--------SNTNVFEHSGKFL 432
                F  A +   +  L  FF    +N+ +     K L        +NT +  H GK L
Sbjct: 147 -----FFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLL 206

Query: 433 SLAENSMAQEIDIM---TLETLGIWDVNGAWNRPFTSHPKKAPETDELVVMGVTPTKPFM 492
           +L E      I ++    L+TLGI D +      FT+HPK  P T E+   G + T P++
Sbjct: 207 ALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYL 266

Query: 493 VVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTIDLNRLIRGGSLI-KY 552
              +IS+DG  M   V + +S   + H+  +T  Y + +D P+      +++   +I  +
Sbjct: 267 TYRVISKDG-IMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSF 326

Query: 553 DKEGYAKIGVMPRYG-DADSIQWFQVKPNC-TMHLFNCFEDEEEVVVWGCRASDSVIPGP 612
           D    A+ GV+PRY  D   I+WF++ PNC   H  N +E+E+EVV+  CR  +      
Sbjct: 327 DPTKKARFGVLPRYAKDELMIRWFEL-PNCFIFHNANAWEEEDEVVLITCRLEN------ 386

Query: 613 EKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKTGEARERSLTGT 672
                            P  D         +E  +  +  YE R N+KTG A ++ L+ +
Sbjct: 387 -----------------PDLDMVSGKVKEKLE--NFGNELYEMRFNMKTGSASQKKLSAS 446

Query: 673 QISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPKK 732
             ++DFP IN  +TG K ++ Y  +LDS A   +G+ KF     LH E          + 
Sbjct: 447 --AVDFPRINECYTGKKQRYVYGTILDSIAKV-TGIIKF----DLHAEAETG-----KRM 506

Query: 733 CEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITNTSQVYIIDAKK 792
            E    IK  Y + E   + S A +V RE + EEDDG++I  VH+E T  S V +IDAK 
Sbjct: 507 LEVGGNIKGIYDLGEGR-YGSEAIYVPRE-TAEEDDGYLIFFVHDENTGKSCVTVIDAKT 523

Query: 793 FSEEAVAKITLPHRVPYGFH 795
            S E VA + LPHRVPYGFH
Sbjct: 567 MSAEPVAVVELPHRVPYGFH 523

BLAST of Sgr027960 vs. ExPASy Swiss-Prot
Match: Q94IR2 (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris OX=3885 GN=CCD1 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 1.2e-55
Identity = 170/553 (30.74%), Postives = 264/553 (47.74%), Query Frame = 0

Query: 272 HPTQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYI 331
           H   GN  PV E      +  ++G +P+    G ++R GPNP F  +         + Y 
Sbjct: 49  HYLTGNFGPVTETPPTKDL-PVKGHLPDCL-NGEFVRVGPNPKFAPV---------AGYH 108

Query: 332 WVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLS 391
           W +G+GM+H L   KD +      Y +R+V+T   + E+    R  F+   +   L  L 
Sbjct: 109 WFDGDGMIHGLRI-KDGKAT----YVSRFVETSRLKQEEYF-GRSKFMKIGDLKGLFGLL 168

Query: 392 AFFLNLLRFGKVNKDLS------NTNVFEHSGKFLSLAENSMAQEIDIM---TLETLGIW 451
              +++LR      DLS      NT +  H GK L+L+E      I +     L+TLG+ 
Sbjct: 169 MVNIHMLRTKLKVLDLSYGGGTTNTALVYHHGKLLALSEADKPYAIKVFEDGDLQTLGML 228

Query: 452 DVNGAWNRPFTSHPKKAPETDELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSL 511
           D +      FT+HPK  P T E+   G   T P++   +IS+DG  M   V + +S   +
Sbjct: 229 DYDKRLGHSFTAHPKVDPFTGEMFSFGYAHTPPYITYRVISKDG-YMHDPVPITISDPIM 288

Query: 512 SHEIGVTRRYNVILDFPVTIDLNRLIRGGSLI-KYDKEGYAKIGVMPRYG-DADSIQWFQ 571
            H+  +T  Y V +D P+      +++  +LI  +D    A+ GV+PRY  D   I+WF+
Sbjct: 289 MHDFAITENYAVFMDLPLIFRPKEMVKNKTLIFSFDSTKKARFGVLPRYAKDEQHIRWFE 348

Query: 572 VKPNC-TMHLFNCFEDEEEVVVWGCRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEEN 631
           + PNC   H  N +E+E+EVV+  CR  +                       P  D+   
Sbjct: 349 L-PNCFIFHNANAWEEEDEVVLITCRLQN-----------------------PKLDNVGG 408

Query: 632 NTDSSIEDGSLFSRAYEWRLNLKTGEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQV 691
                +E+ S  +  YE R N+KTGEA ++ L+ +  ++DFP +N  +TG K ++ Y   
Sbjct: 409 TVQEKLENFS--NELYEMRFNMKTGEASQKKLSAS--TVDFPRVNENYTGRKQRYVYGTT 468

Query: 692 LDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASF 751
           LDS A   +G+ KF     LH E     E     K E    ++  Y +     F S A +
Sbjct: 469 LDSIAKV-TGIIKF----DLHAEPDHGKE-----KLEVGGNVQGLYDL-GPGKFGSEAVY 528

Query: 752 VSREG--SPEEDDGWIIAHVHNEITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFHESD 811
           + R      EEDDG+++  VH+E    S V++IDAK  S + VA + LP+RVPYGFH   
Sbjct: 529 IPRVPGIESEEDDGYLVLFVHDENAGKSFVHVIDAKTMSADPVAVVELPNRVPYGFH--- 540

BLAST of Sgr027960 vs. ExPASy TrEMBL
Match: A0A6J1CDW2 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like OS=Momordica charantia OX=3673 GN=LOC111010347 PE=3 SV=1)

HSP 1 Score: 980.3 bits (2533), Expect = 5.5e-282
Identity = 475/575 (82.61%), Postives = 519/575 (90.26%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP +K KQQ+Q LP KVDIL+NLKN+C+KLLD FVDSVFEFSDQTLHPTQGNLAPVEE
Sbjct: 18  PMIPMRKQKQQKQLLPLKVDILKNLKNSCIKLLDVFVDSVFEFSDQTLHPTQGNLAPVEE 77

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +G AVAVT I+GT+P+EFP+GIYIRNGPNP+FGGLKST S+FGRS+Y WVEGEGMLHALY
Sbjct: 78  IGTAVAVTTIQGTIPQEFPQGIYIRNGPNPLFGGLKSTKSIFGRSNYTWVEGEGMLHALY 137

Query: 344 FNKD--SRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFG 403
           FNKD     KWNLFYNNRYVQT+TF+LEK VKN P FLPA+EGDSLAVLSAFFLN+LRFG
Sbjct: 138 FNKDHTKLRKWNLFYNNRYVQTQTFQLEKHVKNGPYFLPAIEGDSLAVLSAFFLNMLRFG 197

Query: 404 KVNKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPE 463
            VNKD+SNTNV EHSGKF S+A+NS+ Q+IDIM+L++LG+WDVNGAWNRPFTSHPKKAP 
Sbjct: 198 TVNKDMSNTNVIEHSGKFYSVADNSLPQQIDIMSLQSLGVWDVNGAWNRPFTSHPKKAPG 257

Query: 464 TDELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVT 523
           T ELV+MGVTP KPFM++GIISEDGK MVHKVDVKLSR S SHEIGVTRRYNVILD+PVT
Sbjct: 258 TGELVIMGVTPAKPFMLLGIISEDGKEMVHKVDVKLSRGSFSHEIGVTRRYNVILDYPVT 317

Query: 524 IDLNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVV 583
           ID NRLI GGSLIKYDKEGYA+IGVMPRYGD DSIQWFQVKPNCTMHLFN FE  +EVVV
Sbjct: 318 IDFNRLITGGSLIKYDKEGYARIGVMPRYGDGDSIQWFQVKPNCTMHLFNSFEQNDEVVV 377

Query: 584 WGCRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNT-DSSIEDGSLFSRAYEWRLN 643
           WGCRASDSVIPGPEKG NKFEWFS RFKPLP  D + +++  SS+EDGSLFSRAYEWRLN
Sbjct: 378 WGCRASDSVIPGPEKGHNKFEWFSNRFKPLPIADEQNSDSCSSSMEDGSLFSRAYEWRLN 437

Query: 644 LKTGEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLH 703
           LKTGEARER L+GTQ SMDFPFINSRFTGL+NKFGYAQ+LDSFASSNSGMFKFGGLAKLH
Sbjct: 438 LKTGEARERFLSGTQFSMDFPFINSRFTGLQNKFGYAQILDSFASSNSGMFKFGGLAKLH 497

Query: 704 FEEP-QSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHN 763
           FEEP QS  FSLPKK   +  IKVEYHMFEN+SFCSGASFV RE   EEDDGWIIAH+HN
Sbjct: 498 FEEPDQSCGFSLPKKWGNE-AIKVEYHMFENSSFCSGASFVPREDGAEEDDGWIIAHLHN 557

Query: 764 EITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           EITNTSQVYIIDAKKFSEEAVAKITLP RVPYGFH
Sbjct: 558 EITNTSQVYIIDAKKFSEEAVAKITLPQRVPYGFH 591

BLAST of Sgr027960 vs. ExPASy TrEMBL
Match: A0A6J1IV34 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111480255 PE=3 SV=1)

HSP 1 Score: 939.9 bits (2428), Expect = 8.3e-270
Identity = 453/571 (79.33%), Postives = 511/571 (89.49%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ+Q +  +VDI++ LKN CMKL+DGFVD VFEFSDQTLHP Q NLAPVEE
Sbjct: 37  PLIPGKKLKQQQQHV--RVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEE 96

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAVT I+GT+P++FP+GI++RNGPNP+FGGLKST SMFGRSS+IWVEGEGMLHA+Y
Sbjct: 97  IGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMY 156

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K+  GKW+L YNNRYVQTE+FR EK VKNRPSFLPAVEGD++A+++AF LN LRFG+V
Sbjct: 157 FKKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEV 216

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSGKF S+AENSM Q+IDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 217 NKDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 276

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIGVTRR+NVILD+PVTID
Sbjct: 277 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTID 336

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
           LNRLIRGGSLIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMHLFNCFE + EVVVWG
Sbjct: 337 LNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWG 396

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS+IPGPEKGLNKFEWFS+RFKP+  TD  E NT    EDGSL S AYEWRLNLKT
Sbjct: 397 CRATDSIIPGPEKGLNKFEWFSQRFKPVHVTD--EQNT----EDGSLLSCAYEWRLNLKT 456

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GEARER LTG Q SMDFPFINS FTGLKNK+GYAQVLDS ASSNSG+FKFGGLAKLHFEE
Sbjct: 457 GEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEE 516

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGWIIAHVHNEIT 
Sbjct: 517 PQSGEVSLLKDSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITK 576

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 577 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 598

BLAST of Sgr027960 vs. ExPASy TrEMBL
Match: A0A6J1FNM1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111445898 PE=3 SV=1)

HSP 1 Score: 931.4 bits (2406), Expect = 2.9e-267
Identity = 446/571 (78.11%), Postives = 506/571 (88.62%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ      +VDI+Q LKN CMKL+D FVD+VFEFSDQTLHP Q NLAPVEE
Sbjct: 32  PLIPGKKLKQQH----VRVDIVQKLKNGCMKLVDAFVDTVFEFSDQTLHPNQSNLAPVEE 91

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAV  I+GT+P++FP+GI++RNGPNP+FGGLK T SM GRSS+IWVEGEGMLHA+Y
Sbjct: 92  IGAPVAVADIQGTLPQQFPQGIFLRNGPNPLFGGLKWTRSMLGRSSFIWVEGEGMLHAIY 151

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K+  G+W+L+YNNRYVQT++F+ EK VKNRPSFLPAVEGD+LAV++AF LN LRFG+V
Sbjct: 152 FKKNINGEWHLYYNNRYVQTDSFQFEKHVKNRPSFLPAVEGDALAVVAAFVLNWLRFGEV 211

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSG+F S+AENSM QEIDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 212 NKDMSNTNVIEHSGRFYSVAENSMPQEIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 271

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIGVTRR+NVILD+PVTID
Sbjct: 272 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRRFNVILDYPVTID 331

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
           LNRLIRGGSLIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMHLFNCFE + EVVVWG
Sbjct: 332 LNRLIRGGSLIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWG 391

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS+IPGPEKGLNKFEWFS+RFKP+  TD      + + EDGSL S AYEWRLNLKT
Sbjct: 392 CRATDSIIPGPEKGLNKFEWFSQRFKPVHVTD------EQNAEDGSLLSCAYEWRLNLKT 451

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GEARER LTG Q SMDFPFINS FTGLKN++GYAQVLDS ASSNSGMFKFGGLAKLHFEE
Sbjct: 452 GEARERFLTGNQFSMDFPFINSAFTGLKNRYGYAQVLDSVASSNSGMFKFGGLAKLHFEE 511

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGWIIAHVHNEITN
Sbjct: 512 PQSGEVSLLKGSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITN 571

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 572 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 591

BLAST of Sgr027960 vs. ExPASy TrEMBL
Match: A0A6J1J0T1 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111480255 PE=3 SV=1)

HSP 1 Score: 888.6 bits (2295), Expect = 2.2e-254
Identity = 434/571 (76.01%), Postives = 490/571 (85.81%), Query Frame = 0

Query: 224 PIIPTKKPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 283
           P+IP KK KQQ+Q +  +VDI++ LKN CMKL+DGFVD VFEFSDQTLHP Q NLAPVEE
Sbjct: 37  PLIPGKKLKQQQQHV--RVDIVKKLKNGCMKLVDGFVDWVFEFSDQTLHPNQTNLAPVEE 96

Query: 284 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 343
           +GA VAVT I+GT+P++FP+GI++RNGPNP+FGGLKST SMFGRSS+IWVEGEGMLHA+Y
Sbjct: 97  IGAPVAVTDIQGTLPQQFPQGIFLRNGPNPLFGGLKSTRSMFGRSSFIWVEGEGMLHAMY 156

Query: 344 FNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGKV 403
           F K+  GKW+L YNNRYVQTE+FR EK VKNRPSFLPAVEGD++A+++AF LN LRFG+V
Sbjct: 157 FKKNMDGKWHLSYNNRYVQTESFRFEKHVKNRPSFLPAVEGDAVAIVAAFLLNWLRFGEV 216

Query: 404 NKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNGAWNRPFTSHPKKAPETD 463
           NKD+SNTNV EHSGKF S+AENSM Q+IDIM+L++LG+WDV  AWNRPFTSHPKKAP+T 
Sbjct: 217 NKDMSNTNVIEHSGKFYSVAENSMPQQIDIMSLQSLGVWDVCRAWNRPFTSHPKKAPDTG 276

Query: 464 ELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTID 523
           ELV+MG+ PTKP+M +GIISEDGKRMVHKVDVKLSRSSL+HEIGVTRR            
Sbjct: 277 ELVIMGIAPTKPYMEIGIISEDGKRMVHKVDVKLSRSSLTHEIGVTRR------------ 336

Query: 524 LNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFEDEEEVVVWG 583
                    LIK++KEGYAKIGVMPRYGDADSIQWF+VKPNCTMHLFNCFE + EVVVWG
Sbjct: 337 ---------LIKFEKEGYAKIGVMPRYGDADSIQWFEVKPNCTMHLFNCFEHQNEVVVWG 396

Query: 584 CRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKT 643
           CRA+DS+IPGPEKGLNKFEWFS+RFKP+  TD  E NT    EDGSL S AYEWRLNLKT
Sbjct: 397 CRATDSIIPGPEKGLNKFEWFSQRFKPVHVTD--EQNT----EDGSLLSCAYEWRLNLKT 456

Query: 644 GEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEE 703
           GEARER LTG Q SMDFPFINS FTGLKNK+GYAQVLDS ASSNSG+FKFGGLAKLHFEE
Sbjct: 457 GEARERFLTGNQFSMDFPFINSAFTGLKNKYGYAQVLDSVASSNSGIFKFGGLAKLHFEE 516

Query: 704 PQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITN 763
           PQS E SL K  EE+  IKVEYHM ENNSFC+GASFV RE S +EDDGWIIAHVHNEIT 
Sbjct: 517 PQSGEVSLLKDSEEEP-IKVEYHMLENNSFCTGASFVPREDSLQEDDGWIIAHVHNEITK 576

Query: 764 TSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           TSQVYI+DAK+FS+E VAKITLPHRVPYGFH
Sbjct: 577 TSQVYIVDAKRFSDEPVAKITLPHRVPYGFH 577

BLAST of Sgr027960 vs. ExPASy TrEMBL
Match: A0A1S3CRS3 (carotenoid 9,10(9',10')-cleavage dioxygenase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103503600 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 1.6e-236
Identity = 414/581 (71.26%), Postives = 473/581 (81.41%), Query Frame = 0

Query: 225 IIPTKKPKQ-QRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEE 284
           IIPT+K K+  R+ +P KVD+ + LK T MKLLDGFVDSVFEFSDQTLHP Q N APVEE
Sbjct: 37  IIPTEKVKKTDREIIPLKVDVFKKLKTTWMKLLDGFVDSVFEFSDQTLHPNQINFAPVEE 96

Query: 285 MGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALY 344
           MG+AV VT I+GT+P++FP G+YIRNG NP+FGGLK   S+FG SS+IWVEGEGMLHALY
Sbjct: 97  MGSAVTVTNIQGTLPQDFPHGVYIRNGGNPLFGGLKWVKSIFGESSFIWVEGEGMLHALY 156

Query: 345 FNKDSRGK-WNLFYNNRYVQTETFRLEKLVKNRPSFLPAVEGDSLAVLSAFFLNLLRFGK 404
           F K S GK W + YNNR+V+T++FRLEKL K RP FLP VEGDS+AVL AF LN +RFGK
Sbjct: 157 FKKASNGKSWEVLYNNRFVETDSFRLEKLEKKRPCFLPTVEGDSVAVLFAFLLNWVRFGK 216

Query: 405 VNKDLSNTNVFEHSGKFLSLAENSMAQEIDIMTLETLGIWDVNG-AW-NRPFTSHPKKAP 464
            NKD+SNTNVFEHSGKF S+AEN +  +IDI TL TLG WD++  AW NRPFTSHPKKAP
Sbjct: 217 FNKDISNTNVFEHSGKFYSIAENHLPHQIDINTLHTLGNWDLDATAWNNRPFTSHPKKAP 276

Query: 465 ETDELVVMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPV 524
           ET ELV+MGVT TKPFM VGIISEDGKRMVHKVDVKLSRSSLSHEIGVT+RYNVILD+ +
Sbjct: 277 ETGELVIMGVTSTKPFMEVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTKRYNVILDYAL 336

Query: 525 TIDLNRLIRGGSLIKYDKEGYAKIGVMPRYGDADSIQWFQVKPNCTMHLFNCFE-DEEEV 584
           T+D NRLIRGG  +KYDKEGY++IGVMPRYGD+DSI+WF VKPNC MHLFNCFE ++ EV
Sbjct: 337 TMDFNRLIRGGPFLKYDKEGYSRIGVMPRYGDSDSIKWFNVKPNCCMHLFNCFELNQHEV 396

Query: 585 VVWGCRASDSVIPGPEKGLNKFEWFSKRF----KPLPATDHEENNTDSSIEDGSLFSRAY 644
           VVWGCRA+DSVIPGPEKGLNKFEWFS+RF      +   + E+NN       GSL   AY
Sbjct: 397 VVWGCRANDSVIPGPEKGLNKFEWFSQRFNNQNNKIVVNEEEDNN------GGSLLPVAY 456

Query: 645 EWRLNLKTGEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGG 704
           +WRLNL+TGE +E+ L    I MDFPFIN RFTGL NKFGYAQ+L S ASSNSGMFKFGG
Sbjct: 457 QWRLNLRTGEVKEKCLIHQSIFMDFPFINLRFTGLPNKFGYAQLLHSSASSNSGMFKFGG 516

Query: 705 LAKLHFEEPQS-FEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWII 764
           LAKL F++  S  EFSL     +   IKVE HM E NS+CSGASFV RE   EEDDGWII
Sbjct: 517 LAKLQFQQHTSNDEFSL-----DDEEIKVECHMLEENSYCSGASFVPRENGEEEDDGWII 576

Query: 765 AHVHNEITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFHE 796
           AHVHNEITNTSQVY++DA+KFSEE +AKITLP RVPYGFH+
Sbjct: 577 AHVHNEITNTSQVYVLDARKFSEEPIAKITLPQRVPYGFHD 606

BLAST of Sgr027960 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 228.0 bits (580), Expect = 3.1e-59
Identity = 175/560 (31.25%), Postives = 264/560 (47.14%), Query Frame = 0

Query: 253 MKLLDGFVDSVFEFSDQTLHPTQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPN 312
           + LL+  V  +   +   LH   GN AP+ +    V    + G +PE    G ++R GPN
Sbjct: 27  LDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFLPECL-NGEFVRVGPN 86

Query: 313 PVFGGLKSTHSMFGRSSYIWVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTETFRLEKLV 372
           P F  +         + Y W +G+GM+H +   KD +      Y +RYV+T   + E+  
Sbjct: 87  PKFDAV---------AGYHWFDGDGMIHGVRI-KDGKAT----YVSRYVKTSRLKQEE-- 146

Query: 373 KNRPSFLPAVEGDSLAVLSAFF----LNLLRFGKVNKDL--------SNTNVFEHSGKFL 432
                F  A +   +  L  FF    +N+ +     K L        +NT +  H GK L
Sbjct: 147 -----FFGAAKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTYGNGTANTALVYHHGKLL 206

Query: 433 SLAENSMAQEIDIM---TLETLGIWDVNGAWNRPFTSHPKKAPETDELVVMGVTPTKPFM 492
           +L E      I ++    L+TLGI D +      FT+HPK  P T E+   G + T P++
Sbjct: 207 ALQEADKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYL 266

Query: 493 VVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTIDLNRLIRGGSLI-KY 552
              +IS+DG  M   V + +S   + H+  +T  Y + +D P+      +++   +I  +
Sbjct: 267 TYRVISKDG-IMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSF 326

Query: 553 DKEGYAKIGVMPRYG-DADSIQWFQVKPNC-TMHLFNCFEDEEEVVVWGCRASDSVIPGP 612
           D    A+ GV+PRY  D   I+WF++ PNC   H  N +E+E+EVV+  CR  +      
Sbjct: 327 DPTKKARFGVLPRYAKDELMIRWFEL-PNCFIFHNANAWEEEDEVVLITCRLEN------ 386

Query: 613 EKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKTGEARERSLTGT 672
                            P  D         +E  +  +  YE R N+KTG A ++ L+ +
Sbjct: 387 -----------------PDLDMVSGKVKEKLE--NFGNELYEMRFNMKTGSASQKKLSAS 446

Query: 673 QISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPKK 732
             ++DFP IN  +TG K ++ Y  +LDS A   +G+ KF     LH E          + 
Sbjct: 447 --AVDFPRINECYTGKKQRYVYGTILDSIAKV-TGIIKF----DLHAEAETG-----KRM 506

Query: 733 CEEQRIIKVEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITNTSQVYIIDAKK 792
            E    IK  Y + E   + S A +V RE + EEDDG++I  VH+E T  S V +IDAK 
Sbjct: 507 LEVGGNIKGIYDLGEGR-YGSEAIYVPRE-TAEEDDGYLIFFVHDENTGKSCVTVIDAKT 523

Query: 793 FSEEAVAKITLPHRVPYGFH 795
            S E VA + LPHRVPYGFH
Sbjct: 567 MSAEPVAVVELPHRVPYGFH 523

BLAST of Sgr027960 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 186.8 bits (473), Expect = 7.9e-47
Identity = 158/592 (26.69%), Postives = 263/592 (44.43%), Query Frame = 0

Query: 230 KPKQQRQQLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHP----------TQGNLA 289
           KPK  ++    ++++ Q      +   +GF+      S + LHP            GN A
Sbjct: 75  KPK-AKESNTKQMNLFQRAAAAALDAAEGFL-----VSHEKLHPLPKTADPSVQIAGNFA 134

Query: 290 PVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGML 349
           PV E      +  + G +P+   +G+Y+RNG NP+   +   H         + +G+GM+
Sbjct: 135 PVNEQPVRRNLPVV-GKLPDSI-KGVYVRNGANPLHEPVTGHH---------FFDGDGMV 194

Query: 350 HALYFNKDSRGKWNLFYNNRYVQTETFRLEKLVKNRPSFLPAV---EGDSLAVLSAFFLN 409
           HA+ F   S       Y  R+ QT  F  E+ +  RP F  A+    G +       F  
Sbjct: 195 HAVKFEHGSAS-----YACRFTQTNRFVQERQL-GRPVFPKAIGELHGHTGIARLMLFYA 254

Query: 410 LLRFGKVN----KDLSNTNVFEHSGKFLSLAENSMAQEIDIM---TLETLGIWDVNGAWN 469
               G V+      ++N  +   +G+ L+++E+ +  ++ I     L+T+G +D +G   
Sbjct: 255 RAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDGQLE 314

Query: 470 RPFTSHPKKAPETDELVVMGV-TPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGV 529
               +HPK  PE+ EL  +     +KP++     S DG +    V+++L + ++ H+  +
Sbjct: 315 STMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKS-PDVEIQLDQPTMMHDFAI 374

Query: 530 TRRYNVILDFPVTIDLNRLIRGGSLIKYDKEGYAKIGVMPRYG-DADSIQWFQVKPNCTM 589
           T  + V+ D  V   L  +IRGGS + YDK   A+ G++ +Y  D+ +I+W         
Sbjct: 375 TENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCF 434

Query: 590 HLFNCFEDEE--EVVVWGCRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSI 649
           HL+N +E+ E  EVVV G     S +  P+   N+                         
Sbjct: 435 HLWNAWEEPETDEVVVIG-----SCMTPPDSIFNE------------------------- 494

Query: 650 EDGSLFSRAYEWRLNLKTGEARERSLTGT---QISMDFPFINSRFTGLKNKFGYAQVLDS 709
            D +L S   E RLNLKTGE+  R +      Q++++   +N    G K KF Y  + + 
Sbjct: 495 SDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEP 554

Query: 710 FASSNSGMFKFGGLAKLHFEEPQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSR 769
           +        K  G AK+     +                 V+ H++ +N +     F+  
Sbjct: 555 WP-------KVSGFAKVDLTTGE-----------------VKKHLYGDNRYGGEPLFLPG 585

Query: 770 EGSPEEDDGWIIAHVHNEITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           EG  EED+G+I+  VH+E T  S++ I++A     EA  K  LP RVPYGFH
Sbjct: 615 EGG-EEDEGYILCFVHDEKTWKSELQIVNAVSLEVEATVK--LPSRVPYGFH 585

BLAST of Sgr027960 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 175.3 bits (443), Expect = 2.4e-43
Identity = 147/575 (25.57%), Postives = 255/575 (44.35%), Query Frame = 0

Query: 237 QLPSKVDILQNLKNTCMKLLDGFVDSVFEFSDQTLHPTQGNLAPVEEMGAAVAVTAIEGT 296
           +L  ++ +   L  T   +++ F+D     S    H    N APV +         I GT
Sbjct: 74  KLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHGT 133

Query: 297 VPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYIWVEGEGMLHALYFNKDSRGKWNLFY 356
           +P     G YIRNGPNP F           R  Y   +G+GMLHA+   K   GK  L  
Sbjct: 134 LPLSL-NGAYIRNGPNPQF---------LPRGPYHLFDGDGMLHAI---KIHNGKATLC- 193

Query: 357 NNRYVQTETFRLEKL--VKNRPSFLPAVEGDSLAV----LSAFFLNLLRFGKVNK-DLSN 416
            +RYV+T  + +EK       P+      G + +V    L+A  +   ++  VN   L+N
Sbjct: 194 -SRYVKTYKYNVEKQTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLAN 253

Query: 417 TNVFEHSGKFLSLAENSMAQEIDIM---TLETLGIWDVNGAWNRPFTSHPKKAPETDELV 476
           T++   S +  +L E+ +   + +     +ET+G +D +G      T+HPK  P T E  
Sbjct: 254 TSLAFFSNRLFALGESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETF 313

Query: 477 VMGVTPTKPFMVVGIISEDGKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTIDLNR 536
                P  PF+        GK+        ++  S  H+  +T+R+ +  +  + + +N 
Sbjct: 314 AFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNM 373

Query: 537 L---IRGGSLIKYDKEGYAKIGVMPRY-GDADSIQWFQVKPNCTMHLFNCFEDEEEVVVW 596
           L   + GGS +  D     ++GV+P+Y GD   ++WF+V     +H  N +++++     
Sbjct: 374 LDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDD----- 433

Query: 597 GCRASDSVIPGPEKGLNKFEWFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLK 656
               +  V+  P                + + +H     D       + +   + +++L 
Sbjct: 434 ---GNSVVLIAPN---------------IMSIEHTLERMD------LVHALVEKVKIDLV 493

Query: 657 TGEARERSLTGTQISMDFPFINSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFE 716
           TG  R   ++    ++DF  IN  F G  +++ YA + D        M K  G+ KL   
Sbjct: 494 TGIVRRHPISAR--NLDFAVINPAFLGRCSRYVYAAIGDP-------MPKISGVVKLDVS 553

Query: 717 EPQSFEFSLPKKCEEQRIIKVEYHMFENNSFCSGASFVSRE-GSP--EEDDGWIIAHVHN 776
           +    + ++ ++            M+ +  +     FV+R+ G+P  EEDDG+++ +VH+
Sbjct: 554 KGDRDDCTVARR------------MYGSGCYGGEPFFVARDPGNPEAEEDDGYVVTYVHD 583

Query: 777 EITNTSQVYIIDAKKFSEEAVAKITLPHRVPYGFH 795
           E+T  S+  ++DAK    E VA + LP RVPYGFH
Sbjct: 614 EVTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFH 583

BLAST of Sgr027960 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 174.5 bits (441), Expect = 4.1e-43
Identity = 180/691 (26.05%), Postives = 303/691 (43.85%), Query Frame = 0

Query: 159 VPSQMRDFLRGSRISETHLPQVRIRTPRNCSRLRNFEVLLLPLLIASFAFSAVDSIKLKG 218
           +PS    FL  +  + +   + ++++    S LRN + L++P  ++S       S+  K 
Sbjct: 57  LPSTSTQFLDRTFSTSSSSSRPKLQSLSFSSTLRN-KKLVVPCYVSS-------SVNKKS 116

Query: 219 AGIRRPIIPTKKPKQQRQQLPSKVDILQNLKN----------TCMKLLDGFVDSVFEFSD 278
           +       PT KP   ++      +++    N          T   +LD  V++     +
Sbjct: 117 SVSSSLQSPTFKPPSWKKLCNDVTNLIPKTTNQNPKLNPVQRTAAMVLDA-VENAMISHE 176

Query: 279 QTLHP----------TQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGL 338
           +  HP            GN  PV E    V    + GTVPE   +G+Y+RNG NP+   +
Sbjct: 177 RRRHPHPKTADPAVQIAGNFFPVPEK-PVVHNLPVTGTVPECI-QGVYVRNGANPLHKPV 236

Query: 339 KSTHSMFGRSSYIWVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTETFRL-EKLVKNRPS 398
            S H +F        +G+GM+HA+ F+  S     + Y  R+  TET RL ++    RP 
Sbjct: 237 -SGHHLF--------DGDGMVHAVRFDNGS-----VSYACRF--TETNRLVQERECGRPV 296

Query: 399 FLPAVEGDSLAVLSAFFLNLLR----FGKVNK----DLSNTNVFEHSGKFLSLAENSMAQ 458
           F P   G+    L    L L      FG V+      ++N  +   +G  L+++E+ +  
Sbjct: 297 F-PKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPY 356

Query: 459 EIDIM---TLETLGIWDVNGAWNRPFTSHPKKAPETDELVVMGV-TPTKPFMVVGIISED 518
            + +     LET G +D +G       +HPK  PET EL  +     +KP++     + D
Sbjct: 357 HVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSD 416

Query: 519 GKRMVHKVDVKLSRSSLSHEIGVTRRYNVILDFPVTIDLNRLIRGGSLIKYDKEGYAKIG 578
           G++    V++ L + ++ H+  +T  + VI D  V   L  +IRGGS + YD++  ++ G
Sbjct: 417 GEKS-PDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPVVYDEKKKSRFG 476

Query: 579 VMPRYG-DADSIQWFQVKPNCTMHLFNCFEDEE--EVVVWGCRASDSVIPGPEKGLNKFE 638
           ++ +   DA SIQW +V      HL+N +E+ E  EVVV G     S +  P+   N   
Sbjct: 477 ILNKNAKDASSIQWIEVPDCFCFHLWNSWEEPETDEVVVIG-----SCMTPPDSIFN--- 536

Query: 639 WFSKRFKPLPATDHEENNTDSSIEDGSLFSRAYEWRLNLKTGEARERSLTGTQISMDFPF 698
                       +H+E          +L S   E RLNLKTGE+  R +   Q++++   
Sbjct: 537 ------------EHDE----------TLQSVLSEIRLNLKTGESTRRPVISEQVNLEAGM 596

Query: 699 INSRFTGLKNKFGYAQVLDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPKKCEEQRIIK 758
           +N    G K ++ Y  + + +        K  G AK+     +                 
Sbjct: 597 VNRNLLGRKTRYAYLALTEPWP-------KVSGFAKVDLSTGE----------------- 656

Query: 759 VEYHMFENNSFCSGASFVSREGSPEEDDGWIIAHVHNEITNTSQVYIIDAKKFSEEAVAK 814
           +  +++    +  G       G  EED G+I+  VH+E    S++ +I+A     EA   
Sbjct: 657 IRKYIYGEGKY-GGEPLFLPSGDGEEDGGYIMVFVHDEEKVKSELQLINAVNMKLEAT-- 657

BLAST of Sgr027960 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 169.5 bits (428), Expect = 1.3e-41
Identity = 144/542 (26.57%), Postives = 250/542 (46.13%), Query Frame = 0

Query: 272 HPTQGNLAPVEEMGAAVAVTAIEGTVPEEFPEGIYIRNGPNPVFGGLKSTHSMFGRSSYI 331
           H   GN APV E     ++ +++G +P+   +G+Y+RNG NP+F  + S H +F      
Sbjct: 111 HQISGNYAPVPEQSVKSSL-SVDGKIPDCI-DGVYLRNGANPLFEPV-SGHHLF------ 170

Query: 332 WVEGEGMLHALYFNKDSRGKWNLFYNNRYVQTETFRLEKLVKNR--PSFLPAVEGDS-LA 391
             +G+GM+HA+   K + G  +  Y+ R+ +TE    EK + +   P  +  + G S +A
Sbjct: 171 --DGDGMVHAV---KITNG--DASYSCRFTETERLVQEKQLGSPIFPKAIGELHGHSGIA 230

Query: 392 VLSAFFLNLLRFGKVN----KDLSNTNVFEHSGKFLSLAENSMAQEI---DIMTLETLGI 451
            L  F+   L FG +N      ++N  +     + L+++E+ +  ++   D   LET+G 
Sbjct: 231 RLMLFYARGL-FGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLETIGR 290

Query: 452 WDVNGAWNRPFTSHPKKAPETDELVVMGV-TPTKPFMVVGIISEDGKRMVHKVDVKLSRS 511
           +D +G  +    +HPK  P T EL  +      KP++     S +G++    V++ L+  
Sbjct: 291 FDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKS-PDVEIPLASP 350

Query: 512 SLSHEIGVTRRYNVILDFPVTIDLNRLIRGGSLIKYDKEGYAKIGVMPRYG-DADSIQWF 571
           ++ H+  +T  + VI D  V   L+ +  G S +KYD E  ++ G++PR   DA  + W 
Sbjct: 351 TMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMVWV 410

Query: 572 QVKPNCTMHLFNCFED---EEEVVVWGCRA-SDSVIPGPEKGLNKFEWFSKRFKPLPATD 631
           +       HL+N +E    +E VV+  C   +DS+    ++ LN                
Sbjct: 411 ESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLN---------------- 470

Query: 632 HEENNTDSSIEDGSLFSRAYEWRLNLKTGEARERSLT--GTQISMDFPFINSRFTGLKNK 691
                           S   E RLNLKTG++  R++     Q++++   +N    G K +
Sbjct: 471 ----------------SVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTR 530

Query: 692 FGYAQVLDSFASSNSGMFKFGGLAKLHFEEPQSFEFSLPKKCEEQRIIKVEYHMFENNSF 751
           + Y  + + +        K  G AK+     +                 V+ H +    +
Sbjct: 531 YAYLAIAEPWP-------KVSGFAKVDLSTGE-----------------VKNHFYGGKKY 576

Query: 752 CSGASFVSRE-GSPEEDDGWIIAHVHNEITNTSQVYIIDAKKFSEEAVAKITLPHRVPYG 795
                F+ R   S  EDDG+I++ VH+E +  S+++I++A     EA  K  LP RVPYG
Sbjct: 591 GGEPFFLPRGLESDGEDDGYIMSFVHDEESWESELHIVNAVTLELEATVK--LPSRVPYG 576