Sgr027771 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr027771
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionRING-type E3 ubiquitin transferase
Locationtig00153055: 2452424 .. 2454617 (+)
RNA-Seq ExpressionSgr027771
SyntenySgr027771
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAATTAATTTTATCGGTAATTACGGGGATGGGGAGGAAAGAGGGTTTCGTCCCCTGGCCCAAGCGATAGACCATAGCAGTGGGGGGGAGGGACTCGGCAGAACTTAGCTTTTTCTGCTGCTTTCTTTCTGTCATCTATCTCTTTCTCAGTGCCTCCCATTCTTTCGTGTCTTAGATCCTCCAAATTTCTTCTGACACTTTCTATCAAAAAAAAAAAAAAAAAAGAGAGAGAAATTTTGAGAACATTTTAGTGGTTTTTGGGGGAGTGTACTGTTCAAGTATGAGTGATTTACATTCATGGCGTCGCTTTTGATTGCCTCTTTTTTGGGTTTTCATCGTCTGGGTTTGATGATTTCGCCCTTTTTCGGAAGCTTGAAGAGGAAGTGAAGAAGAAGAAGAAGAAGATTACTGGCCCATCATATGTCGTTACAGATGATGATGGAGGCGATGGTGAAGAAGAAGAAGAAGGCCGCTCTTTTGACCAGCCAAAGGGTTCGGATAAAGAAAGAAAGGAAAAGCATCAATGTGAGAAAAGATGTCCCCATTCGGCATTTTTGCTGCTTGTGATTGTCAATGAGAAGGATAGCTATATACTAAAGATAGTGGAAGAAAGAGAAAGTAAGAGAGAAGCAAAAACCAGACTCTTCCTTTTTCTCACCCTTTCTTTTTTTTTTTTTCTCCTTTTTATTCTTCGCACGTATTTTCTGTCATTGCGTTTTGAATCCTTCGTGTCTTGGTTGCTGAAAGTATTCAAAAGCTCGTTGGGTTTTCTATTCCAGGGTTTGGGGTAAATTTTTTTCTCTTGAGATTTGTTTTGTTTTAAGGGTTTTCGAAAATGCCGATGTACCAGCCATCCGGCCGGAGAAGAGAAGCCGATGCGGAGTTGGGTGTGGGTGGTGGTGGGCAAGTCCTCGACGTGGAAACCGCCGTGAAGGACGGTATTCTAGGCGGCGGAGGTGGGTTAATCTGCGACGGCGGAGGGGCTGAGAAATTGGATCTGAAGGAGATGGTAGAGGAGCTCGAGAACATCGAGGTTCCGTCGGTGTTTATTTGCCCAATTTCATTGGAGCCAATGCGAGATCCTGTGACCCTTTGCACAGGCCAGACCTACGAACGATCCAACATCCTCAAATGGTTCTCTCTCGGCCATTTCACATGTCCCACCACAATGCAAGAGCTCTGGGACGATTCAATCACCCCCAACAACACCCTTCACCAACTGATTTACAGTTGGTTTTCGCAGAAGTATTTGGCGATGAAGAAGAGGTCGGAGGACGTGCAAGGGAGGGCTCTGGAGCTCTTAGACACTTTGAAAAAGGTCAAGGGCCAAGCCAGAGTCCGAGCTTTGAAAGAGCTCCGGCAAGTCGTGGCCACAAATTCTTCGGCCAAGAAGACAGTGGAAGACAATGGCGGTCTTGCTTCCATCTCTTCCTTGTTAGGCCCTTTCACTTCCCACGCAGTCGGCTCGGAAGCCATTGGAATCCTCGTGAACTTGGATCTCGATTCCGAGTCCAGGAGAAATCTTGTTCAACCTGCAAAGATTTCATTAATGGTGGACATGTTGAACGAAGGATCCACAGAGACCAAAATCAACTGCACCAGATTGATTGAAACTTTGCTTGAAGGTGATGATTGTGGATCAGAAAGCGTCTCAAGTTTGAGCCTTTTGGTTGGATTGTTAAGATTAATCAAGGAAAAGAAACACCCAAATGGTGTTCTTGCTGGATTAAGATTGCTAAAAGCTCTGTGTTCTCAAGAACCAGTGAGGAACTCAGTAGTGAGCATAGGGGCAATCCCACAGTTGATCGAAATCCTACCAAATTTCGACCCGGAATGTTTGGAACTAGCCCTTCATGTATTGGACTCACTCTCATCCCTCTCAGAGGGCAGGGTGGCTTTGAAAGATTGCCCCAACACCATTCCAAACTTAGTGAAGTTGTTAATGAGAGTCTCAGAGAGCTGCACTCAGCTGGCTTTATCAATCTTGTGGGCAGTTTGCAAGCTTGCCCCTGAAGAATGTGCATCACAAGCTGTGGAGGCAGGCTTAGCAGCCAAACTTCTTCTGGTGATTCAGAGTGGTTGCAATCCCGTTCTGAAACAGCGCTCCGCCGAGCTTCTGAAACTGTGCAGCTTAAATTACACTGCAACCCTCTTCATTTCCAAGTGCAAGCTTACGAGAACGATACAATAA

mRNA sequence

ATGTTAATTAATTTTATCGGTAATTACGGGGATGGGGAGGAAAGAGGGTTTCGTCCCCTGGCCCAAGCGATAGACCATAGCATGATTTACATTCATGGCGTCGCTTTTGATTGCCTCTTTTTTGGGTTTTCATCGTCTGGGTTTGATGATTTCGCCCTTTTTCGGAAGCTTGAAGAGGAAGTGAAGAAGAAGAAGAAGAAGATTACTGGCCCATCATATGTCGTTACAGATGATGATGGAGGCGATGGTGAAGAAGAAGAAGAAGGCCGCTCTTTTGACCAGCCAAAGGGTTCGGATAAAGAAAGAAAGGAAAAGCATCAATGTGAGAAAAGATGTCCCCATTCGGCATTTTTGCTGCTTGTGATTGTCAATGAGAAGGATAGCTATATACTAAAGATAGTGGAAGAAAGAGAAAGGTTTTCGAAAATGCCGATGTACCAGCCATCCGGCCGGAGAAGAGAAGCCGATGCGGAGTTGGGTGTGGGTGGTGGTGGGCAAGTCCTCGACGTGGAAACCGCCGTGAAGGACGGTATTCTAGGCGGCGGAGGTGGGTTAATCTGCGACGGCGGAGGGGCTGAGAAATTGGATCTGAAGGAGATGGTAGAGGAGCTCGAGAACATCGAGGTTCCGTCGGTGTTTATTTGCCCAATTTCATTGGAGCCAATGCGAGATCCTGTGACCCTTTGCACAGGCCAGACCTACGAACGATCCAACATCCTCAAATGGTTCTCTCTCGGCCATTTCACATGTCCCACCACAATGCAAGAGCTCTGGGACGATTCAATCACCCCCAACAACACCCTTCACCAACTGATTTACAGTTGGTTTTCGCAGAAGTATTTGGCGATGAAGAAGAGGTCGGAGGACGTGCAAGGGAGGGCTCTGGAGCTCTTAGACACTTTGAAAAAGGTCAAGGGCCAAGCCAGAGTCCGAGCTTTGAAAGAGCTCCGGCAAGTCGTGGCCACAAATTCTTCGGCCAAGAAGACAGTGGAAGACAATGGCGGTCTTGCTTCCATCTCTTCCTTGTTAGGCCCTTTCACTTCCCACGCAGTCGGCTCGGAAGCCATTGGAATCCTCGTGAACTTGGATCTCGATTCCGAGTCCAGGAGAAATCTTGTTCAACCTGCAAAGATTTCATTAATGGTGGACATGTTGAACGAAGGATCCACAGAGACCAAAATCAACTGCACCAGATTGATTGAAACTTTGCTTGAAGGTGATGATTGTGGATCAGAAAGCGTCTCAAGTTTGAGCCTTTTGGTTGGATTGTTAAGATTAATCAAGGAAAAGAAACACCCAAATGGTGTTCTTGCTGGATTAAGATTGCTAAAAGCTCTGTGTTCTCAAGAACCAGTGAGGAACTCAGTAGTGAGCATAGGGGCAATCCCACAGTTGATCGAAATCCTACCAAATTTCGACCCGGAATGTTTGGAACTAGCCCTTCATGTATTGGACTCACTCTCATCCCTCTCAGAGGGCAGGGTGGCTTTGAAAGATTGCCCCAACACCATTCCAAACTTAGTGAAGTTGTTAATGAGAGTCTCAGAGAGCTGCACTCAGCTGGCTTTATCAATCTTGTGGGCAGTTTGCAAGCTTGCCCCTGAAGAATGTGCATCACAAGCTGTGGAGGCAGGCTTAGCAGCCAAACTTCTTCTGGTGATTCAGAGTGGTTGCAATCCCGTTCTGAAACAGCGCTCCGCCGAGCTTCTGAAACTGTGCAGCTTAAATTACACTGCAACCCTCTTCATTTCCAAGTGCAAGCTTACGAGAACGATACAATAA

Coding sequence (CDS)

ATGTTAATTAATTTTATCGGTAATTACGGGGATGGGGAGGAAAGAGGGTTTCGTCCCCTGGCCCAAGCGATAGACCATAGCATGATTTACATTCATGGCGTCGCTTTTGATTGCCTCTTTTTTGGGTTTTCATCGTCTGGGTTTGATGATTTCGCCCTTTTTCGGAAGCTTGAAGAGGAAGTGAAGAAGAAGAAGAAGAAGATTACTGGCCCATCATATGTCGTTACAGATGATGATGGAGGCGATGGTGAAGAAGAAGAAGAAGGCCGCTCTTTTGACCAGCCAAAGGGTTCGGATAAAGAAAGAAAGGAAAAGCATCAATGTGAGAAAAGATGTCCCCATTCGGCATTTTTGCTGCTTGTGATTGTCAATGAGAAGGATAGCTATATACTAAAGATAGTGGAAGAAAGAGAAAGGTTTTCGAAAATGCCGATGTACCAGCCATCCGGCCGGAGAAGAGAAGCCGATGCGGAGTTGGGTGTGGGTGGTGGTGGGCAAGTCCTCGACGTGGAAACCGCCGTGAAGGACGGTATTCTAGGCGGCGGAGGTGGGTTAATCTGCGACGGCGGAGGGGCTGAGAAATTGGATCTGAAGGAGATGGTAGAGGAGCTCGAGAACATCGAGGTTCCGTCGGTGTTTATTTGCCCAATTTCATTGGAGCCAATGCGAGATCCTGTGACCCTTTGCACAGGCCAGACCTACGAACGATCCAACATCCTCAAATGGTTCTCTCTCGGCCATTTCACATGTCCCACCACAATGCAAGAGCTCTGGGACGATTCAATCACCCCCAACAACACCCTTCACCAACTGATTTACAGTTGGTTTTCGCAGAAGTATTTGGCGATGAAGAAGAGGTCGGAGGACGTGCAAGGGAGGGCTCTGGAGCTCTTAGACACTTTGAAAAAGGTCAAGGGCCAAGCCAGAGTCCGAGCTTTGAAAGAGCTCCGGCAAGTCGTGGCCACAAATTCTTCGGCCAAGAAGACAGTGGAAGACAATGGCGGTCTTGCTTCCATCTCTTCCTTGTTAGGCCCTTTCACTTCCCACGCAGTCGGCTCGGAAGCCATTGGAATCCTCGTGAACTTGGATCTCGATTCCGAGTCCAGGAGAAATCTTGTTCAACCTGCAAAGATTTCATTAATGGTGGACATGTTGAACGAAGGATCCACAGAGACCAAAATCAACTGCACCAGATTGATTGAAACTTTGCTTGAAGGTGATGATTGTGGATCAGAAAGCGTCTCAAGTTTGAGCCTTTTGGTTGGATTGTTAAGATTAATCAAGGAAAAGAAACACCCAAATGGTGTTCTTGCTGGATTAAGATTGCTAAAAGCTCTGTGTTCTCAAGAACCAGTGAGGAACTCAGTAGTGAGCATAGGGGCAATCCCACAGTTGATCGAAATCCTACCAAATTTCGACCCGGAATGTTTGGAACTAGCCCTTCATGTATTGGACTCACTCTCATCCCTCTCAGAGGGCAGGGTGGCTTTGAAAGATTGCCCCAACACCATTCCAAACTTAGTGAAGTTGTTAATGAGAGTCTCAGAGAGCTGCACTCAGCTGGCTTTATCAATCTTGTGGGCAGTTTGCAAGCTTGCCCCTGAAGAATGTGCATCACAAGCTGTGGAGGCAGGCTTAGCAGCCAAACTTCTTCTGGTGATTCAGAGTGGTTGCAATCCCGTTCTGAAACAGCGCTCCGCCGAGCTTCTGAAACTGTGCAGCTTAAATTACACTGCAACCCTCTTCATTTCCAAGTGCAAGCTTACGAGAACGATACAATAA

Protein sequence

MLINFIGNYGDGEERGFRPLAQAIDHSMIYIHGVAFDCLFFGFSSSGFDDFALFRKLEEEVKKKKKKITGPSYVVTDDDGGDGEEEEEGRSFDQPKGSDKERKEKHQCEKRCPHSAFLLLVIVNEKDSYILKIVEERERFSKMPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQVVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKISLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Homology
BLAST of Sgr027771 vs. NCBI nr
Match: KAG7024312.1 (U-box domain-containing protein 31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 854.7 bits (2207), Expect = 4.6e-244
Identity = 462/549 (84.15%), Postives = 488/549 (88.89%), Query Frame = 0

Query: 47  GFDDFALFRKLEEEVKKKKKKITGPSYVVTDDDGGDGEEEEE-GRSFDQPKGSDKERKEK 106
           GF++ ALF                 S+VVTDDDGGDGEEEEE G S DQP+GSD+E KE 
Sbjct: 7   GFEESALF---------------SSSFVVTDDDGGDGEEEEEGGLSSDQPRGSDEETKE- 66

Query: 107 HQCEKRCPHSAF-LLLVIVNEKDSYILKIVEERERFSKMPMYQPSGRRREADAELGVGGG 166
                 CP S F LLL+IV+EK+    KIVEER+RF KMPMYQP         ELGVGGG
Sbjct: 67  ------CPRSPFLLLLLIVSEKN----KIVEERKRFWKMPMYQP---------ELGVGGG 126

Query: 167 GQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVEELENIEVPSVFICPISLEPMRD 226
           GQVLDVETAVKDGIL GGGG IC+GG AEKLDLKEMVEELENIEVPSVFICPISLEPMRD
Sbjct: 127 GQVLDVETAVKDGIL-GGGGFICEGGTAEKLDLKEMVEELENIEVPSVFICPISLEPMRD 186

Query: 227 PVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDDSITPNNTLHQLIYSWFSQKYLAMK 286
           PVTLCTGQTYERSNI+KWFSLGH TCPTTMQELWDDSITPNNTLHQLI+SWFSQKYLAMK
Sbjct: 187 PVTLCTGQTYERSNIMKWFSLGHITCPTTMQELWDDSITPNNTLHQLIHSWFSQKYLAMK 246

Query: 287 KRSEDVQGRALELLDTLKKVKGQARVRALKELRQVVATNSSAKKTVEDNGGLASISSLLG 346
           KRSEDVQGRALELLD LKKVKGQARVRALKELR VVA NSS K TVEDNGGLA ISSLLG
Sbjct: 247 KRSEDVQGRALELLDALKKVKGQARVRALKELRHVVALNSSTKNTVEDNGGLALISSLLG 306

Query: 347 PFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKISLMVDMLNEGSTETKINCTRLIETLL 406
           PFTSHAVGSEAIGILVNLDLD+ESRRNLVQPAKISLMVDMLNEGST+TKINCTRLI+TLL
Sbjct: 307 PFTSHAVGSEAIGILVNLDLDAESRRNLVQPAKISLMVDMLNEGSTDTKINCTRLIQTLL 366

Query: 407 EGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGLRLLKALCSQEPVRNSVVSIGAIPQ 466
           +GDDCG+++VS LSL+VGLLRLIKEK+HPNGVLAGLRLLKALCSQE V+NS+VSIGAIPQ
Sbjct: 367 DGDDCGADTVSGLSLVVGLLRLIKEKQHPNGVLAGLRLLKALCSQEQVKNSIVSIGAIPQ 426

Query: 467 LIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPNTIPNLVKLLMRVSESCTQLALS 526
           L+EILPNF PE LELALHVLDS+SSLS+GRVALKDCP TIPNLVKLLMRVSESCTQLALS
Sbjct: 427 LVEILPNFSPESLELALHVLDSVSSLSDGRVALKDCPKTIPNLVKLLMRVSESCTQLALS 486

Query: 527 ILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKLCSLNYTATLFIS 586
           ILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKLCSLNYTATLFIS
Sbjct: 487 ILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKLCSLNYTATLFIS 519

Query: 587 KCKLTRTIQ 594
           KCKLTRTIQ
Sbjct: 547 KCKLTRTIQ 519

BLAST of Sgr027771 vs. NCBI nr
Match: XP_022141844.1 (U-box domain-containing protein 30-like [Momordica charantia])

HSP 1 Score: 848.2 bits (2190), Expect = 4.3e-242
Identity = 435/451 (96.45%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVE 202
           MPMYQPSGRRREAD+ELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVE
Sbjct: 1   MPMYQPSGRRREADSELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVE 60

Query: 203 ELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDDSI 262
           ELEN+EVPSVFICPISLEPMRDPVTLCTGQTYER+NILKWFSLGHFTCPTTMQELWDDSI
Sbjct: 61  ELENLEVPSVFICPISLEPMRDPVTLCTGQTYERANILKWFSLGHFTCPTTMQELWDDSI 120

Query: 263 TPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQVVAT 322
           TPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQAR+RALKELRQVVA+
Sbjct: 121 TPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARIRALKELRQVVAS 180

Query: 323 NSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKISLMV 382
           NSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNL LDSESRRNLVQPAK+SLMV
Sbjct: 181 NSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLVLDSESRRNLVQPAKVSLMV 240

Query: 383 DMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGLRL 442
           DMLNEGSTETKINCTRLIETLLEGDDCG+E++SSLSLLVGLLRLIKEKKHPNGVLAGLRL
Sbjct: 241 DMLNEGSTETKINCTRLIETLLEGDDCGAENISSLSLLVGLLRLIKEKKHPNGVLAGLRL 300

Query: 443 LKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPN 502
           LK+LCSQEPV NSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPN
Sbjct: 301 LKSLCSQEPVWNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPN 360

Query: 503 TIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVL 562
           TIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECA+QAVEAGLAAKLLLVIQSGCNPVL
Sbjct: 361 TIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECAAQAVEAGLAAKLLLVIQSGCNPVL 420

Query: 563 KQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           KQR +ELLKLCSLNYTAT FISKCKLTRTIQ
Sbjct: 421 KQRCSELLKLCSLNYTATHFISKCKLTRTIQ 451

BLAST of Sgr027771 vs. NCBI nr
Match: KAG7037754.1 (U-box domain-containing protein 30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 832.4 bits (2149), Expect = 2.4e-237
Identity = 434/459 (94.55%), Postives = 445/459 (96.95%), Query Frame = 0

Query: 138 ERFSKMPMYQP-SGRRREADAELGV--GGGGQVLDVETAVKDGILGGGGGLICDGGGAEK 197
           E F KMPMYQP SGRRREAD+E+G+  GGGGQVLDVETAVKDGIL GGGGLICDGGGAEK
Sbjct: 42  EGFVKMPMYQPSSGRRREADSEMGLGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEK 101

Query: 198 LDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTM 257
           LDLKE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTM
Sbjct: 102 LDLKEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTM 161

Query: 258 QELWDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK 317
           QELWDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK
Sbjct: 162 QELWDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK 221

Query: 318 ELRQVVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQ 377
           ELRQVV +NSSAKKTVEDNGGL SI SLLGPFTSHAVGSE IGILVNLDLD+ESRRNLVQ
Sbjct: 222 ELRQVVTSNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEVIGILVNLDLDAESRRNLVQ 281

Query: 378 PAKISLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPN 437
           PAKISLMVDMLNEGSTETKINCTRLIET L+GDDCG+++VSS SLLVGLLRLIKEKKHPN
Sbjct: 282 PAKISLMVDMLNEGSTETKINCTRLIETFLDGDDCGADTVSSFSLLVGLLRLIKEKKHPN 341

Query: 438 GVLAGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGR 497
           GVLAGLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGR
Sbjct: 342 GVLAGLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGR 401

Query: 498 VALKDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVI 557
           VALKDCPNTIPNLVKLLMR+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVI
Sbjct: 402 VALKDCPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVI 461

Query: 558 QSGCNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           QSGCNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 462 QSGCNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 499

BLAST of Sgr027771 vs. NCBI nr
Match: XP_022981987.1 (U-box domain-containing protein 30-like [Cucurbita maxima] >XP_022981988.1 U-box domain-containing protein 30-like [Cucurbita maxima])

HSP 1 Score: 829.7 bits (2142), Expect = 1.6e-236
Identity = 434/456 (95.18%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 143 MPMYQP-SGRRREADAELGV----GGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDL 202
           MPMYQP SGRRREAD+ELG+    GGGGQVLDVETAVKDGIL GGGGLICDGGGAEKLDL
Sbjct: 1   MPMYQPSSGRRREADSELGLGGGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDL 60

Query: 203 KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 262
           KE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL
Sbjct: 61  KEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 120

Query: 263 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 322
           WDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR
Sbjct: 121 WDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180

Query: 323 QVVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAK 382
           QVVA+NSSAKKTVEDNGGL SI SLLGPFTSHAVGSEAIGILVNLDLD+ESRRNLVQPAK
Sbjct: 181 QVVASNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAK 240

Query: 383 ISLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVL 442
           ISLMVDMLNEGSTETKINCTRLIETLL+GDDCG+++VSS SLLVGLLRLIKEKKHPNGVL
Sbjct: 241 ISLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVL 300

Query: 443 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 502
           AGLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL
Sbjct: 301 AGLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 360

Query: 503 KDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 562
           KDCPNTI NLVKLLMR+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG
Sbjct: 361 KDCPNTITNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 420

Query: 563 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 455

BLAST of Sgr027771 vs. NCBI nr
Match: XP_023524197.1 (U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo] >XP_023524198.1 U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 829.7 bits (2142), Expect = 1.6e-236
Identity = 433/456 (94.96%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 143 MPMYQP-SGRRREADAELGV----GGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDL 202
           MPMYQP SGRRREAD+E+G+    GGGGQVLDVETAVKDGIL GGGGLICDGGGAEKLDL
Sbjct: 1   MPMYQPSSGRRREADSEMGLGGGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDL 60

Query: 203 KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 262
           KE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL
Sbjct: 61  KEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 120

Query: 263 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 322
           WDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR
Sbjct: 121 WDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180

Query: 323 QVVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAK 382
           QVVA+NSSAKKTVEDNGGL SI SLLGPFTSHAVGSEAIGILVNLDLD+ESRRNLVQPAK
Sbjct: 181 QVVASNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAK 240

Query: 383 ISLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVL 442
           ISLMVDMLNEGSTETKINCTRLIETLL+GDDCG+++VSS SLLVGLLRLIKEKKHPNGVL
Sbjct: 241 ISLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVL 300

Query: 443 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 502
           AGLRLLKALC QEPV NSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL
Sbjct: 301 AGLRLLKALCCQEPVMNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 360

Query: 503 KDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 562
           KDCPNTIPNLVKLLMR+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG
Sbjct: 361 KDCPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 420

Query: 563 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 455

BLAST of Sgr027771 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 8.6e-169
Identity = 313/455 (68.79%), Postives = 374/455 (82.20%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGV--GGGGQVLDVETAVKDGILGG--GGGLICDGGGAEKLDLK 202
           MPM+QP  R    D  +G   GG GQVLD++TAVKDG+LGG  GGG+   G   EKLDLK
Sbjct: 1   MPMFQPLKR----DGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGV---GVVDEKLDLK 60

Query: 203 EMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELW 262
           +M++EL+  ++PSVFICPISLEPM+DPVTLCTGQTYER NI KWF+LGH TCPTTMQELW
Sbjct: 61  KMIKELDLQDIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELW 120

Query: 263 DDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 322
           DD++TPN TLH LIY+WFSQKY+ MKKRSEDVQGRA+E+L TLKK KGQARV AL EL+Q
Sbjct: 121 DDTVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQ 180

Query: 323 VVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKI 382
           +V  +  A+KTV + GG++ ISSLLGPFTSHAVGSE + ILV+LDLDS+S+  L+QPAK+
Sbjct: 181 IVIAHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKV 240

Query: 383 SLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLA 442
           SL+VDMLN+GS ETKINC RLI  L+E     +E VSS SLLVGL+RL+K+K+H NGV  
Sbjct: 241 SLIVDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSP 300

Query: 443 GLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALK 502
            LRLLK +   + VR+ +VSIGA+PQL++ILP+ DPECLELAL VLD+L +  EGRVA+K
Sbjct: 301 ALRLLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVK 360

Query: 503 DCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGC 562
           D  NTIP  V++LMRVSE+CT  ALSILW+VCKLAPEEC+  AVE GLAAKLLLVIQSGC
Sbjct: 361 DSANTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGC 420

Query: 563 NPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           +  LKQRSAELLKLCSL+Y+ T+FISKCKLTRTIQ
Sbjct: 421 DAALKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of Sgr027771 vs. ExPASy Swiss-Prot
Match: Q9FHN9 (U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 7.5e-165
Identity = 306/453 (67.55%), Postives = 364/453 (80.35%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGILGGGGG--LICDGGGAEKLDLKEM 202
           MPM+QPS            G  G +LD+ +AVKDG+LGGG G  L+      +KLDLKEM
Sbjct: 1   MPMFQPSKNG---------GFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEM 60

Query: 203 VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDD 262
           + ELE  E+PSVFICPISLEPM+DPVTLCTGQTYERSNILKWF++GH TCPTTMQELWDD
Sbjct: 61  ISELELPEIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 263 SITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQVV 322
            +TPN TLHQLIY+WFSQKY+ MKKRSEDVQGRA+E+L TL+K KG+A+V AL EL+QVV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 323 ATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKISL 382
             ++ AKKTV D GG+  ISSLL PFTSHAVGSEAI ILVNL+LDS+S+  L+QPA++SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 383 MVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGL 442
           MVDMLN+GS ETKINC RLI  L+E     +E VSS SLLVGL+RL+K+++  NGV   L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 443 RLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDC 502
            LLK++   + VRN +V IGA+PQL+++LP  D ECLE AL VLDSL   SEGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 503 PNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNP 562
            NTIP+ V+LLM+VSE CT  A+SILW+VCKLA EEC+S AVE GLAAKLLLVIQSGC+P
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 563 VLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
            LKQRSAELLKLCSL+Y+ ++FISKCKLTRTIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of Sgr027771 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 4.3e-144
Identity = 263/450 (58.44%), Postives = 349/450 (77.56%), Query Frame = 0

Query: 164 GGQVLDVETAVKDGILGGG---GGLICDGGGAEKLDLKEMVEELE-------NIEVPSVF 223
           GGQVLD++TA+KDGILGGG   G      GG + ++L++M++EL+       +  VP+VF
Sbjct: 10  GGQVLDIDTALKDGILGGGPELGDAAAGDGGKQPVELRKMMDELDAAGDGGGDEAVPAVF 69

Query: 224 ICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDDSITPNNTLHQLIY 283
           ICPISLEPM DPVTLCTGQTYE +NI +W +LGH TCPTTMQELWD +  PN TL QLI 
Sbjct: 70  ICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTLRQLIA 129

Query: 284 SWFSQKYLAMKKRSEDVQGRALELLDTLK--------KVKGQARVRALKELRQVVATNSS 343
           +WFS++Y   KKRS D  GRA EL+  L+         +KGQARV AL+ELR + A + S
Sbjct: 130 AWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVAALRELRSLAAAHQS 189

Query: 344 AKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVN-LDLDSESRRNLVQPAKISLMVDM 403
             K + + GG+  ++SLLGPFTSHAVGSEA+ ILV+ + LD++++  L+QPAK+SL+VDM
Sbjct: 190 VTKAIAEAGGVGLLTSLLGPFTSHAVGSEAVAILVSGVPLDADAKAALMQPAKVSLLVDM 249

Query: 404 LNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGLRLLK 463
           LNEG+ +TKINC RLI  L+E      ++V+SLSLLVG++RL+++K+HP+GV AGL LL 
Sbjct: 250 LNEGAVDTKINCVRLIRILMEEKGFRPDTVASLSLLVGVMRLVRDKRHPDGVAAGLELLN 309

Query: 464 ALCS-QEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPNT 523
           ++C+  +P R+ +VSIGA+PQL+E+LP    EC+E AL +LD+L+++ EGR+ALKDCP T
Sbjct: 310 SICAVHKPARSLIVSIGAVPQLVELLPELPTECVEPALDILDALAAVPEGRIALKDCPRT 369

Query: 524 IPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLK 583
           I N V+LLMRVSE+CT+ ALS+LW VC++APEECA  A++AGL AKLLLVIQSGC P LK
Sbjct: 370 ITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPAALDAGLGAKLLLVIQSGCGPELK 429

Query: 584 QRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           Q+++ELLKLC++N T+T+FISKCKLT+TIQ
Sbjct: 430 QQASELLKLCTMNCTSTVFISKCKLTKTIQ 459

BLAST of Sgr027771 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.6e-37
Identity = 127/394 (32.23%), Postives = 202/394 (51.27%), Query Frame = 0

Query: 201 VEELE-NIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLG-HFTCPTTMQELW 260
           +E L+  I++P  F CPISLE M+DPVT+CTGQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 261 DDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLKKVKG----- 320
           D ++ PN+TL +LI  W         ++    K+ ++    RA  LL     + G     
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRA--LLSQASAITGTHVSV 124

Query: 321 QARVRALKELRQVVATNSSAKKTV-EDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLD 380
           ++R  AL+ LR     +   +  +   N     I  L    TS  + SE++ +LV L + 
Sbjct: 125 RSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPIT 184

Query: 381 SESRRNLV--QPAKISLMVDMLNEGSTETKINCTRLIETLLEGD---DCGSESVSSLSLL 440
             ++   +   P ++  +  +L + S ET++N   LIE +  G    D      +S S+ 
Sbjct: 185 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVF 244

Query: 441 VGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE-ILPNFDPECLE 500
            G+L L++        L  G++ L ALCS +  R+  ++ GA   LI+ +  +FD    E
Sbjct: 245 EGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTE 304

Query: 501 LALHVLDSLSSLSEGRVALKDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECA 560
            AL  ++ L    EG  A  +   T+P LVK ++RVS+  T+ A   L A+C  A E   
Sbjct: 305 RALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TAEERWR 364

Query: 561 SQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKL 573
            +A  AG+  +LLL++QS C    K+++ +LLKL
Sbjct: 365 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKL 395

BLAST of Sgr027771 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 1.3e-36
Identity = 125/406 (30.79%), Postives = 202/406 (49.75%), Query Frame = 0

Query: 190 GGAEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFT 249
           G  E LDL         I++P  F CPISL+ M DPVT+ TGQTY+R++I  W ++G+ T
Sbjct: 3   GNLEPLDL--------GIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTT 62

Query: 250 CPTTMQELWDDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLK 309
           CP T   L D ++ PN+TL +LI  W         ++    K+ ++ +  R+  LL    
Sbjct: 63  CPVTRVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRS--LLSQAS 122

Query: 310 KVKG-----QARVRALKELRQVVATNSSAKKTVEDNGGLASISSLL-----GPFTSHAVG 369
            + G     ++R  A++ LR +   +   +  +  +     +  +L         S  + 
Sbjct: 123 AITGTHVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV 182

Query: 370 SEAIGILVNLDL-DSESRRNLVQPAKISLMVDMLNEGSTETKINCTRLIETLLEGD---D 429
           SE++ +LV L + ++E       P+++  M  +L + S E ++N   LIE +L G    D
Sbjct: 183 SESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 242

Query: 430 CGSESVSSLSLLVGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE 489
                  S S+  G+L L+K        L  G++ + ALC  +  R+  +S GA   LI+
Sbjct: 243 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 302

Query: 490 -ILPNFDPECLELALHVLDSLSSLSEGRVALKDCPNTIPNLVKLLMRVSESCTQLALSIL 549
            +  +FD    E  L  ++ L  L EG  A  +   T+P +VK ++RVS+  T+ A   L
Sbjct: 303 RLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL 362

Query: 550 WAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKL 573
            A+C  A E C  +A  AGL  +LLL++QS C    K+++  LLKL
Sbjct: 363 LALC-TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKL 397

BLAST of Sgr027771 vs. ExPASy TrEMBL
Match: A0A6J1CJY0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111012109 PE=4 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 2.1e-242
Identity = 435/451 (96.45%), Postives = 447/451 (99.11%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVE 202
           MPMYQPSGRRREAD+ELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVE
Sbjct: 1   MPMYQPSGRRREADSELGVGGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKEMVE 60

Query: 203 ELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDDSI 262
           ELEN+EVPSVFICPISLEPMRDPVTLCTGQTYER+NILKWFSLGHFTCPTTMQELWDDSI
Sbjct: 61  ELENLEVPSVFICPISLEPMRDPVTLCTGQTYERANILKWFSLGHFTCPTTMQELWDDSI 120

Query: 263 TPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQVVAT 322
           TPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQAR+RALKELRQVVA+
Sbjct: 121 TPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARIRALKELRQVVAS 180

Query: 323 NSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKISLMV 382
           NSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNL LDSESRRNLVQPAK+SLMV
Sbjct: 181 NSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLVLDSESRRNLVQPAKVSLMV 240

Query: 383 DMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGLRL 442
           DMLNEGSTETKINCTRLIETLLEGDDCG+E++SSLSLLVGLLRLIKEKKHPNGVLAGLRL
Sbjct: 241 DMLNEGSTETKINCTRLIETLLEGDDCGAENISSLSLLVGLLRLIKEKKHPNGVLAGLRL 300

Query: 443 LKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPN 502
           LK+LCSQEPV NSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPN
Sbjct: 301 LKSLCSQEPVWNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDCPN 360

Query: 503 TIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVL 562
           TIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECA+QAVEAGLAAKLLLVIQSGCNPVL
Sbjct: 361 TIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECAAQAVEAGLAAKLLLVIQSGCNPVL 420

Query: 563 KQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           KQR +ELLKLCSLNYTAT FISKCKLTRTIQ
Sbjct: 421 KQRCSELLKLCSLNYTATHFISKCKLTRTIQ 451

BLAST of Sgr027771 vs. ExPASy TrEMBL
Match: A0A6J1IVG5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480973 PE=4 SV=1)

HSP 1 Score: 829.7 bits (2142), Expect = 7.6e-237
Identity = 434/456 (95.18%), Postives = 444/456 (97.37%), Query Frame = 0

Query: 143 MPMYQP-SGRRREADAELGV----GGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDL 202
           MPMYQP SGRRREAD+ELG+    GGGGQVLDVETAVKDGIL GGGGLICDGGGAEKLDL
Sbjct: 1   MPMYQPSSGRRREADSELGLGGGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDL 60

Query: 203 KEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 262
           KE+VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL
Sbjct: 61  KEIVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQEL 120

Query: 263 WDDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 322
           WDDSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR
Sbjct: 121 WDDSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELR 180

Query: 323 QVVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAK 382
           QVVA+NSSAKKTVEDNGGL SI SLLGPFTSHAVGSEAIGILVNLDLD+ESRRNLVQPAK
Sbjct: 181 QVVASNSSAKKTVEDNGGLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAK 240

Query: 383 ISLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVL 442
           ISLMVDMLNEGSTETKINCTRLIETLL+GDDCG+++VSS SLLVGLLRLIKEKKHPNGVL
Sbjct: 241 ISLMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVL 300

Query: 443 AGLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 502
           AGLRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL
Sbjct: 301 AGLRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVAL 360

Query: 503 KDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 562
           KDCPNTI NLVKLLMR+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG
Sbjct: 361 KDCPNTITNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSG 420

Query: 563 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 CNPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 455

BLAST of Sgr027771 vs. ExPASy TrEMBL
Match: A0A6J1FKS4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446650 PE=4 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 1.0e-236
Identity = 433/454 (95.37%), Postives = 444/454 (97.80%), Query Frame = 0

Query: 143 MPMYQP-SGRRREADAELGV--GGGGQVLDVETAVKDGILGGGGGLICDGGGAEKLDLKE 202
           MPMYQP SGRRREAD+E+G+  GGGGQVLDVETAVKDGIL GGGGLICDGGGAEKLDLKE
Sbjct: 1   MPMYQPSSGRRREADSEMGLGGGGGGQVLDVETAVKDGIL-GGGGLICDGGGAEKLDLKE 60

Query: 203 MVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWD 262
           +VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWD
Sbjct: 61  IVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWD 120

Query: 263 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV 322
           DSITPNNTLHQLIYSWF QKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV
Sbjct: 121 DSITPNNTLHQLIYSWFWQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV 180

Query: 323 VATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKIS 382
           VA+NSSAKKTVEDNG L SI SLLGPFTSHAVGSEAIGILVNLDLD+ESRRNLVQPAKIS
Sbjct: 181 VASNSSAKKTVEDNGVLGSILSLLGPFTSHAVGSEAIGILVNLDLDAESRRNLVQPAKIS 240

Query: 383 LMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAG 442
           LMVDMLNEGSTETKINCTRLIETLL+GDDCG+++VSS SLLVGLLRLIKEKKHPNGVLAG
Sbjct: 241 LMVDMLNEGSTETKINCTRLIETLLDGDDCGADTVSSFSLLVGLLRLIKEKKHPNGVLAG 300

Query: 443 LRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 502
           LRLLKALC QEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD
Sbjct: 301 LRLLKALCCQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 360

Query: 503 CPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCN 562
           CPNTIPNLVKLLMR+SESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCN
Sbjct: 361 CPNTIPNLVKLLMRISESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCN 420

Query: 563 PVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           PVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 PVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Sgr027771 vs. ExPASy TrEMBL
Match: A0A5A7VFI6 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold255G001750 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 4.6e-234
Identity = 425/454 (93.61%), Postives = 440/454 (96.92%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGIL---GGGGGLICDGGGAEKLDLKE 202
           MPMYQPSGRRRE D ELG+GGGGQVLDVETAVKDGIL   GGGGGLIC+GG  EKLDLKE
Sbjct: 1   MPMYQPSGRRREDDTELGLGGGGQVLDVETAVKDGILGGGGGGGGLICEGGMTEKLDLKE 60

Query: 203 MVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWD 262
           +VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGH TCPTTMQELWD
Sbjct: 61  IVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHITCPTTMQELWD 120

Query: 263 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV 322
           DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK+LRQV
Sbjct: 121 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKDLRQV 180

Query: 323 VATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKIS 382
           VA N SAK TVEDNGG+A ISSLLGPFTSHAVGSEA+GILVNLDLD+ESRRNLVQPAKIS
Sbjct: 181 VALN-SAKSTVEDNGGVALISSLLGPFTSHAVGSEALGILVNLDLDAESRRNLVQPAKIS 240

Query: 383 LMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAG 442
           LMVDMLNEGST+TKINCTRLIETLL GDDCG+++VSSLSLLVGLLRLIKEKKH NGVLAG
Sbjct: 241 LMVDMLNEGSTDTKINCTRLIETLLNGDDCGTDTVSSLSLLVGLLRLIKEKKHQNGVLAG 300

Query: 443 LRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 502
           LRLLKALCSQEPVRNS+VSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD
Sbjct: 301 LRLLKALCSQEPVRNSLVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 360

Query: 503 CPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCN 562
           CPNTIPNLVKLLMR+SESCTQLALSILW+VCKLAPEECASQAVEAGLAAKLLLVIQSGCN
Sbjct: 361 CPNTIPNLVKLLMRISESCTQLALSILWSVCKLAPEECASQAVEAGLAAKLLLVIQSGCN 420

Query: 563 PVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           P+LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 PILKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Sgr027771 vs. ExPASy TrEMBL
Match: A0A1S3BTT1 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103493618 PE=4 SV=1)

HSP 1 Score: 820.5 bits (2118), Expect = 4.6e-234
Identity = 425/454 (93.61%), Postives = 440/454 (96.92%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGIL---GGGGGLICDGGGAEKLDLKE 202
           MPMYQPSGRRRE D ELG+GGGGQVLDVETAVKDGIL   GGGGGLIC+GG  EKLDLKE
Sbjct: 1   MPMYQPSGRRREDDTELGLGGGGQVLDVETAVKDGILGGGGGGGGLICEGGMTEKLDLKE 60

Query: 203 MVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWD 262
           +VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGH TCPTTMQELWD
Sbjct: 61  IVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHITCPTTMQELWD 120

Query: 263 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQV 322
           DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALK+LRQV
Sbjct: 121 DSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKDLRQV 180

Query: 323 VATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKIS 382
           VA N SAK TVEDNGG+A ISSLLGPFTSHAVGSEA+GILVNLDLD+ESRRNLVQPAKIS
Sbjct: 181 VALN-SAKSTVEDNGGVALISSLLGPFTSHAVGSEALGILVNLDLDAESRRNLVQPAKIS 240

Query: 383 LMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAG 442
           LMVDMLNEGST+TKINCTRLIETLL GDDCG+++VSSLSLLVGLLRLIKEKKH NGVLAG
Sbjct: 241 LMVDMLNEGSTDTKINCTRLIETLLNGDDCGTDTVSSLSLLVGLLRLIKEKKHQNGVLAG 300

Query: 443 LRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 502
           LRLLKALCSQEPVRNS+VSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD
Sbjct: 301 LRLLKALCSQEPVRNSLVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKD 360

Query: 503 CPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCN 562
           CPNTIPNLVKLLMR+SESCTQLALSILW+VCKLAPEECASQAVEAGLAAKLLLVIQSGCN
Sbjct: 361 CPNTIPNLVKLLMRISESCTQLALSILWSVCKLAPEECASQAVEAGLAAKLLLVIQSGCN 420

Query: 563 PVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           P+LKQRSAELLKLCSLNYTATLFISKCKLTRTIQ
Sbjct: 421 PILKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 453

BLAST of Sgr027771 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 595.1 bits (1533), Expect = 6.1e-170
Identity = 313/455 (68.79%), Postives = 374/455 (82.20%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGV--GGGGQVLDVETAVKDGILGG--GGGLICDGGGAEKLDLK 202
           MPM+QP  R    D  +G   GG GQVLD++TAVKDG+LGG  GGG+   G   EKLDLK
Sbjct: 1   MPMFQPLKR----DGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGV---GVVDEKLDLK 60

Query: 203 EMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELW 262
           +M++EL+  ++PSVFICPISLEPM+DPVTLCTGQTYER NI KWF+LGH TCPTTMQELW
Sbjct: 61  KMIKELDLQDIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELW 120

Query: 263 DDSITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQ 322
           DD++TPN TLH LIY+WFSQKY+ MKKRSEDVQGRA+E+L TLKK KGQARV AL EL+Q
Sbjct: 121 DDTVTPNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQ 180

Query: 323 VVATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKI 382
           +V  +  A+KTV + GG++ ISSLLGPFTSHAVGSE + ILV+LDLDS+S+  L+QPAK+
Sbjct: 181 IVIAHLMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKV 240

Query: 383 SLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLA 442
           SL+VDMLN+GS ETKINC RLI  L+E     +E VSS SLLVGL+RL+K+K+H NGV  
Sbjct: 241 SLIVDMLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSP 300

Query: 443 GLRLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALK 502
            LRLLK +   + VR+ +VSIGA+PQL++ILP+ DPECLELAL VLD+L +  EGRVA+K
Sbjct: 301 ALRLLKPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVK 360

Query: 503 DCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGC 562
           D  NTIP  V++LMRVSE+CT  ALSILW+VCKLAPEEC+  AVE GLAAKLLLVIQSGC
Sbjct: 361 DSANTIPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGC 420

Query: 563 NPVLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
           +  LKQRSAELLKLCSL+Y+ T+FISKCKLTRTIQ
Sbjct: 421 DAALKQRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of Sgr027771 vs. TAIR 10
Match: AT5G65920.1 (ARM repeat superfamily protein )

HSP 1 Score: 582.0 bits (1499), Expect = 5.3e-166
Identity = 306/453 (67.55%), Postives = 364/453 (80.35%), Query Frame = 0

Query: 143 MPMYQPSGRRREADAELGVGGGGQVLDVETAVKDGILGGGGG--LICDGGGAEKLDLKEM 202
           MPM+QPS            G  G +LD+ +AVKDG+LGGG G  L+      +KLDLKEM
Sbjct: 1   MPMFQPSKNG---------GFDGHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEM 60

Query: 203 VEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFTCPTTMQELWDD 262
           + ELE  E+PSVFICPISLEPM+DPVTLCTGQTYERSNILKWF++GH TCPTTMQELWDD
Sbjct: 61  ISELELPEIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 263 SITPNNTLHQLIYSWFSQKYLAMKKRSEDVQGRALELLDTLKKVKGQARVRALKELRQVV 322
            +TPN TLHQLIY+WFSQKY+ MKKRSEDVQGRA+E+L TL+K KG+A+V AL EL+QVV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 323 ATNSSAKKTVEDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLDSESRRNLVQPAKISL 382
             ++ AKKTV D GG+  ISSLL PFTSHAVGSEAI ILVNL+LDS+S+  L+QPA++SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 383 MVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPNGVLAGL 442
           MVDMLN+GS ETKINC RLI  L+E     +E VSS SLLVGL+RL+K+++  NGV   L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 443 RLLKALCSQEPVRNSVVSIGAIPQLIEILPNFDPECLELALHVLDSLSSLSEGRVALKDC 502
            LLK++   + VRN +V IGA+PQL+++LP  D ECLE AL VLDSL   SEGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 503 PNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNP 562
            NTIP+ V+LLM+VSE CT  A+SILW+VCKLA EEC+S AVE GLAAKLLLVIQSGC+P
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 563 VLKQRSAELLKLCSLNYTATLFISKCKLTRTIQ 594
            LKQRSAELLKLCSL+Y+ ++FISKCKLTRTIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of Sgr027771 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 159.1 bits (401), Expect = 1.1e-38
Identity = 127/394 (32.23%), Postives = 202/394 (51.27%), Query Frame = 0

Query: 201 VEELE-NIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLG-HFTCPTTMQELW 260
           +E L+  I++P  F CPISLE M+DPVT+CTGQTY+R++I  W S+G + TCP T   L 
Sbjct: 5   IEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLS 64

Query: 261 DDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLKKVKG----- 320
           D ++ PN+TL +LI  W         ++    K+ ++    RA  LL     + G     
Sbjct: 65  DFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRA--LLSQASAITGTHVSV 124

Query: 321 QARVRALKELRQVVATNSSAKKTV-EDNGGLASISSLLGPFTSHAVGSEAIGILVNLDLD 380
           ++R  AL+ LR     +   +  +   N     I  L    TS  + SE++ +LV L + 
Sbjct: 125 RSRAAALRRLRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPIT 184

Query: 381 SESRRNLV--QPAKISLMVDMLNEGSTETKINCTRLIETLLEGD---DCGSESVSSLSLL 440
             ++   +   P ++  +  +L + S ET++N   LIE +  G    D      +S S+ 
Sbjct: 185 EPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVF 244

Query: 441 VGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE-ILPNFDPECLE 500
            G+L L++        L  G++ L ALCS +  R+  ++ GA   LI+ +  +FD    E
Sbjct: 245 EGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTE 304

Query: 501 LALHVLDSLSSLSEGRVALKDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKLAPEECA 560
            AL  ++ L    EG  A  +   T+P LVK ++RVS+  T+ A   L A+C  A E   
Sbjct: 305 RALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TAEERWR 364

Query: 561 SQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKL 573
            +A  AG+  +LLL++QS C    K+++ +LLKL
Sbjct: 365 EEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKL 395

BLAST of Sgr027771 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 156.0 bits (393), Expect = 9.5e-38
Identity = 125/406 (30.79%), Postives = 202/406 (49.75%), Query Frame = 0

Query: 190 GGAEKLDLKEMVEELENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLGHFT 249
           G  E LDL         I++P  F CPISL+ M DPVT+ TGQTY+R++I  W ++G+ T
Sbjct: 3   GNLEPLDL--------GIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTT 62

Query: 250 CPTTMQELWDDSITPNNTLHQLIYSWF-------SQKYLAMKKRSEDVQGRALELLDTLK 309
           CP T   L D ++ PN+TL +LI  W         ++    K+ ++ +  R+  LL    
Sbjct: 63  CPVTRVALSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPISVRS--LLSQAS 122

Query: 310 KVKG-----QARVRALKELRQVVATNSSAKKTVEDNGGLASISSLL-----GPFTSHAVG 369
            + G     ++R  A++ LR +   +   +  +  +     +  +L         S  + 
Sbjct: 123 AITGTHVSVRSRAAAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV 182

Query: 370 SEAIGILVNLDL-DSESRRNLVQPAKISLMVDMLNEGSTETKINCTRLIETLLEGD---D 429
           SE++ +LV L + ++E       P+++  M  +L + S E ++N   LIE +L G    D
Sbjct: 183 SESLALLVLLHMTETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMD 242

Query: 430 CGSESVSSLSLLVGLLRLIKEKKHPNGVL-AGLRLLKALCSQEPVRNSVVSIGAIPQLIE 489
                  S S+  G+L L+K        L  G++ + ALC  +  R+  +S GA   LI+
Sbjct: 243 LKLIISGSDSIFEGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILID 302

Query: 490 -ILPNFDPECLELALHVLDSLSSLSEGRVALKDCPNTIPNLVKLLMRVSESCTQLALSIL 549
            +  +FD    E  L  ++ L  L EG  A  +   T+P +VK ++RVS+  T+ A   L
Sbjct: 303 RLAADFDRCDTERGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGAL 362

Query: 550 WAVCKLAPEECASQAVEAGLAAKLLLVIQSGCNPVLKQRSAELLKL 573
            A+C  A E C  +A  AGL  +LLL++QS C    K+++  LLKL
Sbjct: 363 LALC-TAEERCRDEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKL 397

BLAST of Sgr027771 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 149.4 bits (376), Expect = 8.9e-36
Identity = 110/383 (28.72%), Postives = 197/383 (51.44%), Query Frame = 0

Query: 205 ENIEVPSVFICPISLEPMRDPVTLCTGQTYERSNILKWFSLG-HFTCPTTMQELWDDSIT 264
           E IE+P  F+CPISLE M+DPV + TG TY+R +I KW   G   +CP T Q++ D  +T
Sbjct: 8   EEIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLT 67

Query: 265 PNNTLHQLIYSWFSQKYLAMKKR----SEDVQGRALELLDTLKKVKGQARVRALKELRQV 324
           PN+TL +LI SW +       +R       +    +E L        + +V+ LK LRQ+
Sbjct: 68  PNHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLIRDSASSHENQVKCLKRLRQI 127

Query: 325 VATNSSAKKTVEDNGGLASISSLLGPFTSH-AVGSEAIGILVNLDLDSESRRNLVQPAK- 384
           V+ N++ K+ +E  G    +++++   + + ++  EA+ +L +L+      +NL+   K 
Sbjct: 128 VSENATNKRCLEAAGVPEFLANIVSNDSENGSLTDEALNLLYHLETSETVLKNLLNNKKD 187

Query: 385 ---ISLMVDMLNEGSTETKINCTRLIETLLEGDDCGSESVSSLSLLVGLLRLIKEKKHPN 444
              +  +  ++  G  E+++  T L++ +LE  D          +   +++++ ++    
Sbjct: 188 NNIVKSLTKIMQRGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRISQK 247

Query: 445 GVLAGLRLLKALCSQEPVRNSVVSIGAIPQLIEIL--PNFDPECL--ELALHVLDSLSSL 504
              A + +L  +C     R+  V  G I  +IE+L   +F  E    E+A+ VLD L   
Sbjct: 248 ATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLLCQC 307

Query: 505 SEGRVALKDCPNTIPNLVKLLMRVSESCTQLALSILWAVCKL-APEECASQAVEAGLAAK 564
           +EGR    +    I  + K ++RVS++ +  A+ +L +V +  A      + ++ G+ AK
Sbjct: 308 AEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLLSVGRFCATPALLHEMLQLGVVAK 367

Query: 565 LLLVIQSGCNPVLKQRSAELLKL 573
           L LV+Q  C    K+++ ELLKL
Sbjct: 368 LCLVLQVSCGGKTKEKAKELLKL 390

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7024312.14.6e-24484.15U-box domain-containing protein 31, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022141844.14.3e-24296.45U-box domain-containing protein 30-like [Momordica charantia][more]
KAG7037754.12.4e-23794.55U-box domain-containing protein 30, partial [Cucurbita argyrosperma subsp. argyr... [more]
XP_022981987.11.6e-23695.18U-box domain-containing protein 30-like [Cucurbita maxima] >XP_022981988.1 U-box... [more]
XP_023524197.11.6e-23694.96U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo] >XP_0235241... [more]
Match NameE-valueIdentityDescription
Q058P48.6e-16968.79U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1... [more]
Q9FHN97.5e-16567.55U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2... [more]
Q10PI94.3e-14458.44U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=T... [more]
Q9LT791.6e-3732.23U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA41.3e-3630.79U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1CJY02.1e-24296.45RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11101210... [more]
A0A6J1IVG57.6e-23795.18RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111480973 P... [more]
A0A6J1FKS41.0e-23695.37RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111446650... [more]
A0A5A7VFI64.6e-23493.61RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3BTT14.6e-23493.61RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103493618 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G49810.16.1e-17068.79ARM repeat superfamily protein [more]
AT5G65920.15.3e-16667.55ARM repeat superfamily protein [more]
AT3G19380.11.1e-3832.23plant U-box 25 [more]
AT1G49780.19.5e-3830.79plant U-box 26 [more]
AT2G35930.18.9e-3628.72plant U-box 23 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 212..275
e-value: 3.2E-30
score: 116.4
IPR003613U box domainPFAMPF04564U-boxcoord: 209..279
e-value: 6.4E-15
score: 55.1
IPR003613U box domainPROSITEPS51698U_BOXcoord: 208..282
score: 39.922409
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 194..287
e-value: 2.0E-25
score: 90.6
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 288..583
e-value: 1.6E-22
score: 81.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 72..107
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..107
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 182..592
NoneNo IPR availablePANTHERPTHR22849:SF142U-BOX DOMAIN-CONTAINING PROTEIN 30coord: 182..592
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 213..255
e-value: 3.3717E-24
score: 93.4522
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 203..279
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 379..576
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 298..409

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr027771.1Sgr027771.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:1900457 regulation of brassinosteroid mediated signaling pathway
cellular_component GO:0005886 plasma membrane
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0004842 ubiquitin-protein transferase activity