Homology
BLAST of Sgr027703 vs. NCBI nr
Match:
XP_022138324.1 (uncharacterized protein LOC111009535 isoform X1 [Momordica charantia] >XP_022138331.1 uncharacterized protein LOC111009535 isoform X1 [Momordica charantia] >XP_022138341.1 uncharacterized protein LOC111009535 isoform X1 [Momordica charantia])
HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 608/795 (76.48%), Postives = 668/795 (84.03%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MGF NGESEDEVGA AIRALG LFKLTEVFLWDDETE ARRAERSLAPDADD++NE+ GE
Sbjct: 1 MGFDNGESEDEVGAPAIRALGPLFKLTEVFLWDDETEAARRAERSLAPDADDTHNEDLGE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
K+CSTIS ISLLPED EL +QMN LGLPLSFHTNKEKRNGITTGKR+AT+KHSRIQQGLL
Sbjct: 61 KVCSTISGISLLPEDTELTQQMNALGLPLSFHTNKEKRNGITTGKRRATIKHSRIQQGLL 120
Query: 121 DKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDI 180
DKEMEFP+ SS EEIVAN+NFNDDAIGS+CC SMVDQSE SDC HV DGDI
Sbjct: 121 DKEMEFPRVSSGEEIVANLNFNDDAIGSLCCASMVDQSEASDC---------HVFLDGDI 180
Query: 181 SPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPR 240
PNSTGLIS VEEQS ++M D++LNNGEDHE S GNAVLG+H+ VTSSSISLD+ HSP+
Sbjct: 181 LPNSTGLISSTVEEQSHNLMIDSLLNNGEDHESSPGNAVLGNHSIVTSSSISLDKVHSPK 240
Query: 241 RYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDY 300
RYMIG+DVSH E +EPPME EGSS+TL DTEVQKI+IDSGIGLPLVAESS L+ME Y
Sbjct: 241 RYMIGIDVSH--SEALEPPMELEGSSMTLQDTEVQKINIDSGIGLPLVAESSLLHMEAGY 300
Query: 301 NQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKS 360
++NDHVVGCIH+ GEWMVY DSFYMRNYFYNI ++ESTWNPP GLEHFALSDA+CTAN+S
Sbjct: 301 DENDHVVGCIHEFGEWMVYWDSFYMRNYFYNIKSHESTWNPPAGLEHFALSDADCTANES 360
Query: 361 IAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAF 420
IAEVAE+DV+EDVKS+DI SVLG+T L EG IL+ES ETS F
Sbjct: 361 IAEVAEMDVVEDVKSQDICSVLGET------------------LSEGSSILIESPETSGF 420
Query: 421 VDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHK 480
++T N HKHSD+PHD Q SCRNTR DIGCSCEG+ KQLC E+C +DFQLT AN A E K
Sbjct: 421 INTSTNSHKHSDDPHDCQTSCRNTRKDIGCSCEGHVKQLCREHCSNDFQLTFANGALEQK 480
Query: 481 PFSLGKPNNIDSPEIDCVTR---DDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTIC 540
FSLGKP+NIDSPEID VTR DDDEG M +YTSS S +QQAD DS M FGNG TIC
Sbjct: 481 TFSLGKPSNIDSPEIDIVTRDDYDDDEGTMSIYTSSVSHMIQQADQRDSGMDFGNGRTIC 540
Query: 541 TLGTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRF 600
TLG E+NLA +RKKKMKR RRRRQL +RNEEFQS A TEEYP SIAKYWCQRYQLFSRF
Sbjct: 541 TLGAEKNLAGISRKKKMKRIRRRRQLSERNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
DDGVKMD+EGWFSVTPEPIA+HHALRCG +LIVDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDEEGWFSVTPEPIAQHHALRCGCNLIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
Query: 661 IKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTM 720
KIRYA HNA+IYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDY KVDTYDLKTM
Sbjct: 661 RKIRYAHHNASIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYTKVDTYDLKTM 720
Query: 721 LKPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIEFC 780
LKPHDGYFLFNIAKKIAPFVVMFLPRNVN+DQLAELSLS+ PPWSLE+E+ L +++
Sbjct: 721 LKPHDGYFLFNIAKKIAPFVVMFLPRNVNVDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 766
Query: 781 IVKGCHIHANMCNGL 793
+ N NG+
Sbjct: 781 TAYFSNGSTNEPNGI 766
BLAST of Sgr027703 vs. NCBI nr
Match:
XP_038897817.1 (uncharacterized protein LOC120085727 isoform X2 [Benincasa hispida])
HSP 1 Score: 1199.5 bits (3102), Expect = 0.0e+00
Identity = 599/775 (77.29%), Postives = 660/775 (85.16%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG + ESEDE G SAIRALGSLFKLTEVFLWDDETEVARR E SLA DADD+NNE FGE
Sbjct: 1 MGCSDEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNENFGE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
KIC+TIS ISLLPEDIEL EQMN LGLPLSFHTNKEKR GIT GKRKATVKHSR QQG L
Sbjct: 61 KICTTISGISLLPEDIELTEQMNALGLPLSFHTNKEKRIGITMGKRKATVKHSRTQQGFL 120
Query: 121 DKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDI 180
DKE+EFP ASS+EEIVANINFNDDAIGS+C SMV+QSE SD DVVLD NESHV G+I
Sbjct: 121 DKEVEFPNASSREEIVANINFNDDAIGSLCYLSMVNQSEKSDRDVVLDANESHVISGGNI 180
Query: 181 SPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPR 240
SPN + LISGAVEEQSC VM + VLNNG DHELSLG+AVLGDHT+V SSSI L + HSPR
Sbjct: 181 SPNLSVLISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHTEVRSSSIGLVKVHSPR 240
Query: 241 RYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDY 300
M GLDV HGKQEEVEPPME EGSS+TL DTEVQK DIDSGIGLP+V ESS L+ E DY
Sbjct: 241 MCMTGLDVDHGKQEEVEPPMELEGSSMTLQDTEVQKSDIDSGIGLPIVPESSLLHTEADY 300
Query: 301 NQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKS 360
N++DHVVGCI +SGEWMVY DSFY RNYFYN+ T+ESTWNPP GLEHFA SDAN T N+
Sbjct: 301 NEDDHVVGCIDESGEWMVYWDSFYKRNYFYNMKTHESTWNPPLGLEHFAFSDANFTENEP 360
Query: 361 IAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAF 420
A V ++DVLED+KSEDI VL DT+SCMN +GD++HCQPPDALLEG ++ E +E A
Sbjct: 361 SAGVVQMDVLEDIKSEDI-CVLDDTRSCMNLIGDNVHCQPPDALLEGSSVVAEGSERVAS 420
Query: 421 VDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHK 480
V+T +N +K SDEP + Q+SC+N +IGCSCEG+ KQLCHENC + FQL VAN A E K
Sbjct: 421 VNTPVNSYKQSDEPQERQMSCKNIGENIGCSCEGHVKQLCHENCSNGFQLIVANVASEQK 480
Query: 481 PFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTLG 540
F KP+N++SPE+DCVT DDDEGA+GL TSSAS QQADHMD DM+FGNGPTICTLG
Sbjct: 481 TFGHCKPSNMNSPEMDCVTIDDDEGAVGLATSSASHMPQQADHMDGDMYFGNGPTICTLG 540
Query: 541 TEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDG 600
TEQNL+ RNRKKKMKRTRRR QL +RNE F S A TEEYP SI KYWCQRYQLFSRFDDG
Sbjct: 541 TEQNLSGRNRKKKMKRTRRRGQLSNRNEAFHSLAITEEYPTSITKYWCQRYQLFSRFDDG 600
Query: 601 VKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
VKMDKEGWFSVTPEPIARHHA RCGS+ I+DSFTGVGGNAIQFSQRAKHVIAIDIDPIKI
Sbjct: 601 VKMDKEGWFSVTPEPIARHHASRCGSNTIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
Query: 661 RYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLKP 720
RYAQHNAAIYGV DQIDFIKGDFF LAP LKADVIFLSPPWGGPDYA+VD YDLKTMLKP
Sbjct: 661 RYAQHNAAIYGVGDQIDFIKGDFFCLAPHLKADVIFLSPPWGGPDYARVDIYDLKTMLKP 720
Query: 721 HDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
HDGYFLFNIAKKIAP +VMFLP+NV+LDQLAELSLS+ PPWSLE+E+ L +++
Sbjct: 721 HDGYFLFNIAKKIAPVIVMFLPKNVSLDQLAELSLSSDPPWSLEVEKNFLNGKLK 774
BLAST of Sgr027703 vs. NCBI nr
Match:
XP_038897816.1 (uncharacterized protein LOC120085727 isoform X1 [Benincasa hispida])
HSP 1 Score: 1194.9 bits (3090), Expect = 0.0e+00
Identity = 599/776 (77.19%), Postives = 660/776 (85.05%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG + ESEDE G SAIRALGSLFKLTEVFLWDDETEVARR E SLA DADD+NNE FGE
Sbjct: 1 MGCSDEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNENFGE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKE-KRNGITTGKRKATVKHSRIQQGL 120
KIC+TIS ISLLPEDIEL EQMN LGLPLSFHTNKE KR GIT GKRKATVKHSR QQG
Sbjct: 61 KICTTISGISLLPEDIELTEQMNALGLPLSFHTNKEQKRIGITMGKRKATVKHSRTQQGF 120
Query: 121 LDKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGD 180
LDKE+EFP ASS+EEIVANINFNDDAIGS+C SMV+QSE SD DVVLD NESHV G+
Sbjct: 121 LDKEVEFPNASSREEIVANINFNDDAIGSLCYLSMVNQSEKSDRDVVLDANESHVISGGN 180
Query: 181 ISPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSP 240
ISPN + LISGAVEEQSC VM + VLNNG DHELSLG+AVLGDHT+V SSSI L + HSP
Sbjct: 181 ISPNLSVLISGAVEEQSCDVMCNFVLNNGGDHELSLGDAVLGDHTEVRSSSIGLVKVHSP 240
Query: 241 RRYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPD 300
R M GLDV HGKQEEVEPPME EGSS+TL DTEVQK DIDSGIGLP+V ESS L+ E D
Sbjct: 241 RMCMTGLDVDHGKQEEVEPPMELEGSSMTLQDTEVQKSDIDSGIGLPIVPESSLLHTEAD 300
Query: 301 YNQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANK 360
YN++DHVVGCI +SGEWMVY DSFY RNYFYN+ T+ESTWNPP GLEHFA SDAN T N+
Sbjct: 301 YNEDDHVVGCIDESGEWMVYWDSFYKRNYFYNMKTHESTWNPPLGLEHFAFSDANFTENE 360
Query: 361 SIAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSA 420
A V ++DVLED+KSEDI VL DT+SCMN +GD++HCQPPDALLEG ++ E +E A
Sbjct: 361 PSAGVVQMDVLEDIKSEDI-CVLDDTRSCMNLIGDNVHCQPPDALLEGSSVVAEGSERVA 420
Query: 421 FVDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEH 480
V+T +N +K SDEP + Q+SC+N +IGCSCEG+ KQLCHENC + FQL VAN A E
Sbjct: 421 SVNTPVNSYKQSDEPQERQMSCKNIGENIGCSCEGHVKQLCHENCSNGFQLIVANVASEQ 480
Query: 481 KPFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTL 540
K F KP+N++SPE+DCVT DDDEGA+GL TSSAS QQADHMD DM+FGNGPTICTL
Sbjct: 481 KTFGHCKPSNMNSPEMDCVTIDDDEGAVGLATSSASHMPQQADHMDGDMYFGNGPTICTL 540
Query: 541 GTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDD 600
GTEQNL+ RNRKKKMKRTRRR QL +RNE F S A TEEYP SI KYWCQRYQLFSRFDD
Sbjct: 541 GTEQNLSGRNRKKKMKRTRRRGQLSNRNEAFHSLAITEEYPTSITKYWCQRYQLFSRFDD 600
Query: 601 GVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
GVKMDKEGWFSVTPEPIARHHA RCGS+ I+DSFTGVGGNAIQFSQRAKHVIAIDIDPIK
Sbjct: 601 GVKMDKEGWFSVTPEPIARHHASRCGSNTIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
Query: 661 IRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLK 720
IRYAQHNAAIYGV DQIDFIKGDFF LAP LKADVIFLSPPWGGPDYA+VD YDLKTMLK
Sbjct: 661 IRYAQHNAAIYGVGDQIDFIKGDFFCLAPHLKADVIFLSPPWGGPDYARVDIYDLKTMLK 720
Query: 721 PHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
PHDGYFLFNIAKKIAP +VMFLP+NV+LDQLAELSLS+ PPWSLE+E+ L +++
Sbjct: 721 PHDGYFLFNIAKKIAPVIVMFLPKNVSLDQLAELSLSSDPPWSLEVEKNFLNGKLK 775
BLAST of Sgr027703 vs. NCBI nr
Match:
XP_022975956.1 (uncharacterized protein LOC111476503 isoform X2 [Cucurbita maxima])
HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 597/775 (77.03%), Postives = 653/775 (84.26%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG N ESEDE G SAIRA+GSLFKLTEVFLWDDETEVARR E SLA DADD+NNE+F E
Sbjct: 1 MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
KICSTI+DISL PEDI+L EQMN LGLPLSFHTNKE+R GIT GKRK TVKHSRIQ G L
Sbjct: 61 KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQHGFL 120
Query: 121 DKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDI 180
DKE+EFPK SS+ EIVANIN ND+AIGS+CC+SMV+QSE SDCD V + NESHV FDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
Query: 181 SPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPR 240
SPNS+GLI GAVEEQSC+V D VLNN DHE G+A+LGDH KV S I LD+ HSPR
Sbjct: 181 SPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHAKVRLSPIGLDKGHSPR 240
Query: 241 RYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDY 300
M G DVSHGKQEEVE PME EGSS TL DTEVQKIDIDSGIGLPLVAE S+L+M DY
Sbjct: 241 ICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGADY 300
Query: 301 NQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKS 360
N+NDHVVGCI + GEW VY DSFYMRNYFYNI T+ESTWNPPPGLEHFA SDAN T N+S
Sbjct: 301 NENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENES 360
Query: 361 IAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAF 420
IAEVAE+DVLED K EDI SVL DT+SCMN GD+IHCQPPDALLEG ILVE +++ A
Sbjct: 361 IAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRAS 420
Query: 421 VDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHK 480
V T IN + DEPH+W SCRNTR I CSCEG+ KQ CHENC + FQL VAN E K
Sbjct: 421 VHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQK 480
Query: 481 PFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTLG 540
FS K +N+DSPE VT DDEGA+GL TSS S LQQADHMD DMHFGN PTICTLG
Sbjct: 481 TFSHCKSSNMDSPEKAFVT-IDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLG 540
Query: 541 TEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDG 600
TEQNL+ R+RKKKMKRTRRR QL DRNEEF S A TEEYP SI KYWCQRYQLFSRFDDG
Sbjct: 541 TEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDDG 600
Query: 601 VKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
VKMDKEGWFSVTPE IARHHA RCGS++I+DSFTGVGGNAIQFSQRAKHVIAIDIDPIKI
Sbjct: 601 VKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
Query: 661 RYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLKP 720
RYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YA+VD YDLKTML+P
Sbjct: 661 RYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRP 720
Query: 721 HDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
HDGYFLFNIAKKIAP VVMFLP+NVNLDQLAE++LS++PPWSLE+E+ L +++
Sbjct: 721 HDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLK 772
BLAST of Sgr027703 vs. NCBI nr
Match:
XP_023535337.1 (uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1192.6 bits (3084), Expect = 0.0e+00
Identity = 598/775 (77.16%), Postives = 653/775 (84.26%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG N ESEDE G SAIRALGSLFKLTEVFLWDDETEVARR E SLA DADD+NNE+F E
Sbjct: 1 MGSSNEESEDEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
KICSTI+DISL PEDIEL EQMN LGLPLSFHTNKE+R GIT GKRK TVKHSRIQQG L
Sbjct: 61 KICSTITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQQGFL 120
Query: 121 DKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDI 180
DKE+EFPK SS+ EIVANIN ND+AIGS+CC+SMV+QSE SDCD V + NESHV FDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
Query: 181 SPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPR 240
SPNS+GLI GAVEEQ C V D VLNN DHE G+AVLGDH KV SSI LD+ HSPR
Sbjct: 181 SPNSSGLIHGAVEEQFCDVTCDIVLNNRGDHE--SGDAVLGDHAKVRLSSIGLDKGHSPR 240
Query: 241 RYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDY 300
M G DVSHGKQEEVE PME EGSS TL DTEVQKIDIDSGIGLPLVAE SFL+M DY
Sbjct: 241 ICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADY 300
Query: 301 NQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKS 360
N+NDHVVGCI + GEW VY DSFYMRNYFYNI T+ESTWNPPPGLEHFA DAN T N+S
Sbjct: 301 NENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHFDANFTENES 360
Query: 361 IAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAF 420
IAEVAE+DVLED K EDI SVL DT+SCMN GD+IHCQPPDALLE GILVE +++ A
Sbjct: 361 IAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLESSGILVEGSKSRAS 420
Query: 421 VDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHK 480
V+T I+ + DEPH+W SCRNTR I CSCEG+ KQ CHENC + FQL VAN A E K
Sbjct: 421 VNTSIHSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQK 480
Query: 481 PFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTLG 540
FS KP+N+ +PE VT DDEGA+GL TSS S LQQADHMD DMHFGN PTICTLG
Sbjct: 481 TFSHCKPSNMYAPEKAFVT-IDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLG 540
Query: 541 TEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDG 600
TEQNL+ R+RKKKMKRTRRR QL D+NEEF S A TEEYP SI KYWCQRYQLFSRFDDG
Sbjct: 541 TEQNLSGRDRKKKMKRTRRRGQLSDKNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDG 600
Query: 601 VKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
VKMDKEGWFSVTPE IARHHA RCGS++I+DSFTGVGGNAIQFSQRAKHVIAIDIDP KI
Sbjct: 601 VKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPTKI 660
Query: 661 RYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLKP 720
RYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YA+VD YDLKTML+P
Sbjct: 661 RYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRP 720
Query: 721 HDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
HDGYFLFNIAKKIAP VVMFLP+NVNLDQLAE++LS++PPWSLE+E+ L +++
Sbjct: 721 HDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLK 772
BLAST of Sgr027703 vs. ExPASy Swiss-Prot
Match:
P85107 (Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1)
HSP 1 Score: 239.2 bits (609), Expect = 1.7e-61
Identity = 121/238 (50.84%), Postives = 163/238 (68.49%), Query Frame = 0
Query: 542 EQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDGV 601
E + ++ +KKK K+ ++N++ +AKYW QRY+LFSRFDDG+
Sbjct: 605 EAEVEIKKKKKKKKK--------NKNKKINGLPPEIASVPELAKYWAQRYRLFSRFDDGI 664
Query: 602 KMDKEGWFSVTPEPIARHHALRCGSS----LIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 661
K+DKEGWFSVTPE IA H A R S +IVD+F GVGGN IQF+ K VIAIDIDP
Sbjct: 665 KLDKEGWFSVTPEKIAEHIAGRVSQSFNCDIIVDAFCGVGGNTIQFALTGKRVIAIDIDP 724
Query: 662 IKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTM 721
+KI A++NA +YGV D+I+FI GDF LAP LKADV+FLSPPWGGPDYA +T+D++TM
Sbjct: 725 VKIDLARNNAEVYGVADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTM 784
Query: 722 LKPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
+ P DG+ +F +++KI +V FLPRN ++DQ+A L A P +E+EQ L +++
Sbjct: 785 MSP-DGFEIFRLSQKITNNIVYFLPRNADVDQVASL---AGPGGQVEIEQNFLNNKLK 830
BLAST of Sgr027703 vs. ExPASy Swiss-Prot
Match:
Q96RS0 (Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3)
HSP 1 Score: 233.4 bits (594), Expect = 9.3e-60
Identity = 111/197 (56.35%), Postives = 146/197 (74.11%), Query Frame = 0
Query: 583 IAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALRCGSS----LIVDSFTGVGG 642
+AKYW QRY+LFSRFDDG+K+D+EGWFSVTPE IA H A R S ++VD+F GVGG
Sbjct: 644 LAKYWAQRYRLFSRFDDGIKLDREGWFSVTPEKIAEHIAGRVSQSFKCDVVVDAFCGVGG 703
Query: 643 NAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLS 702
N IQF+ VIAIDIDP+KI A++NA +YG+ D+I+FI GDF LA LKADV+FLS
Sbjct: 704 NTIQFALTGMRVIAIDIDPVKIALARNNAEVYGIADKIEFICGDFLLLASFLKADVVFLS 763
Query: 703 PPWGGPDYAKVDTYDLKTMLKPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAH 762
PPWGGPDYA +T+D++TM+ P DG+ +F ++KKI +V FLPRN ++DQ+A L A
Sbjct: 764 PPWGGPDYATAETFDIRTMMSP-DGFEIFRLSKKITNNIVYFLPRNADIDQVASL---AG 823
Query: 763 PPWSLELEQFLLQVEIE 776
P +E+EQ L +++
Sbjct: 824 PGGQVEIEQNFLNNKLK 836
BLAST of Sgr027703 vs. ExPASy Swiss-Prot
Match:
Q923W1 (Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2)
HSP 1 Score: 231.9 bits (590), Expect = 2.7e-59
Identity = 113/209 (54.07%), Postives = 149/209 (71.29%), Query Frame = 0
Query: 552 KKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDGVKMDKEGWFSV 611
KK K+ + +++ D E S +AKYW QRY+LFSRFDDG+K+DKEGWFSV
Sbjct: 612 KKKKKKNKNKKINDLPPEIAS-------VPELAKYWAQRYRLFSRFDDGIKLDKEGWFSV 671
Query: 612 TPEPIARH------HALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQH 671
TPE IA H A RC ++VD+F GVGGN IQF+ K VIAIDIDP+KI A++
Sbjct: 672 TPEKIAEHIAGRVSQAFRC--DVVVDAFCGVGGNTIQFALTGKRVIAIDIDPVKIDLARN 731
Query: 672 NAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLKPHDGYF 731
NA +YG+ D+I+FI GDF LAP LKADV+FLSPPWGGPDYA +T+D++TM+ P DG+
Sbjct: 732 NAEVYGIADKIEFICGDFLLLAPCLKADVVFLSPPWGGPDYATAETFDIRTMMSP-DGFE 791
Query: 732 LFNIAKKIAPFVVMFLPRNVNLDQLAELS 755
+F +++KI +V FLPRN ++DQ+A L+
Sbjct: 792 IFRLSQKITNNIVYFLPRNADIDQVASLA 810
BLAST of Sgr027703 vs. ExPASy Swiss-Prot
Match:
Q09814 (Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tgs1 PE=1 SV=3)
HSP 1 Score: 152.5 bits (384), Expect = 2.1e-35
Identity = 83/212 (39.15%), Postives = 121/212 (57.08%), Query Frame = 0
Query: 560 RRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARH 619
+ + D +E + P ++ KYW RY LFSRFD+G+ +D + W+SVTPE +A
Sbjct: 3 KNEELDEDELLKKCIICPPVPKALKKYWNNRYNLFSRFDEGIWLDYQSWYSVTPEKVAVA 62
Query: 620 HALR----CGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGV-ED 679
A LI+D+F+G GGN IQF++ VI+I+IDPIKI A+HN IYG+
Sbjct: 63 IAKSVVDFIQPELIIDAFSGCGGNTIQFAKYCP-VISIEIDPIKIAMAKHNLEIYGIPSS 122
Query: 680 QIDFIKGDFFRLAPSLK-----ADVIFLSPPWGGPDYAKVDTYDLKTMLKPHDGYFLFNI 739
++ FI+GD SL+ ++F+SPPWGGP Y+ Y L L P+ LF
Sbjct: 123 RVTFIQGDVLDTFKSLQFAKDYRSLVFMSPPWGGPSYSGKTVYSLND-LNPYAFDVLFKE 182
Query: 740 AKKIAPFVVMFLPRNVNLDQLAELSLSAHPPW 762
A +I+P+V FLPRN ++ +LA + P+
Sbjct: 183 ATRISPYVAAFLPRNTDVKELAAYGSIHNKPY 212
BLAST of Sgr027703 vs. ExPASy Swiss-Prot
Match:
Q12052 (Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TGS1 PE=1 SV=1)
HSP 1 Score: 124.8 bits (312), Expect = 4.6e-27
Identity = 78/224 (34.82%), Postives = 115/224 (51.34%), Query Frame = 0
Query: 550 RKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDD-GVKMDKEGW 609
RK+K R N+ ++ +++ + KYW R +LFS+ D + M E W
Sbjct: 16 RKRKHHSNFRTLIKLLNNDAYKIESSKPLKNGKLFKYWKNRRRLFSKIDSASIYMTDELW 75
Query: 610 FSVTPEPIARH-----HALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYA 669
FSVTPE IA A + I+D F G GGN IQF+ + +V +D I
Sbjct: 76 FSVTPERIACFLANFVKACMPNAERILDVFCGGGGNTIQFAMQFPYVYGVDYSIEHIYCT 135
Query: 670 QHNAAIYGVEDQIDFIKGDFFRLA-----PSLKADVIFLSPPWGGPDYAKVDTYDLKTML 729
NA YGV+D+I +G + +L +K D +F SPPWGGP+Y + D YDL+ L
Sbjct: 136 AKNAQSYGVDDRIWLKRGSWKKLVSKQKLSKIKYDCVFGSPPWGGPEYLRNDVYDLEQHL 195
Query: 730 KPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWS 763
KP + K++P V+MFLPRN +L+QL+ + P++
Sbjct: 196 KPMGITKMLKSFLKLSPNVIMFLPRNSDLNQLSRATRKVLGPFA 239
BLAST of Sgr027703 vs. ExPASy TrEMBL
Match:
A0A6J1C9F8 (Trimethylguanosine synthase OS=Momordica charantia OX=3673 GN=LOC111009535 PE=4 SV=1)
HSP 1 Score: 1205.7 bits (3118), Expect = 0.0e+00
Identity = 608/795 (76.48%), Postives = 668/795 (84.03%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MGF NGESEDEVGA AIRALG LFKLTEVFLWDDETE ARRAERSLAPDADD++NE+ GE
Sbjct: 1 MGFDNGESEDEVGAPAIRALGPLFKLTEVFLWDDETEAARRAERSLAPDADDTHNEDLGE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
K+CSTIS ISLLPED EL +QMN LGLPLSFHTNKEKRNGITTGKR+AT+KHSRIQQGLL
Sbjct: 61 KVCSTISGISLLPEDTELTQQMNALGLPLSFHTNKEKRNGITTGKRRATIKHSRIQQGLL 120
Query: 121 DKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDI 180
DKEMEFP+ SS EEIVAN+NFNDDAIGS+CC SMVDQSE SDC HV DGDI
Sbjct: 121 DKEMEFPRVSSGEEIVANLNFNDDAIGSLCCASMVDQSEASDC---------HVFLDGDI 180
Query: 181 SPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPR 240
PNSTGLIS VEEQS ++M D++LNNGEDHE S GNAVLG+H+ VTSSSISLD+ HSP+
Sbjct: 181 LPNSTGLISSTVEEQSHNLMIDSLLNNGEDHESSPGNAVLGNHSIVTSSSISLDKVHSPK 240
Query: 241 RYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDY 300
RYMIG+DVSH E +EPPME EGSS+TL DTEVQKI+IDSGIGLPLVAESS L+ME Y
Sbjct: 241 RYMIGIDVSH--SEALEPPMELEGSSMTLQDTEVQKINIDSGIGLPLVAESSLLHMEAGY 300
Query: 301 NQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKS 360
++NDHVVGCIH+ GEWMVY DSFYMRNYFYNI ++ESTWNPP GLEHFALSDA+CTAN+S
Sbjct: 301 DENDHVVGCIHEFGEWMVYWDSFYMRNYFYNIKSHESTWNPPAGLEHFALSDADCTANES 360
Query: 361 IAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAF 420
IAEVAE+DV+EDVKS+DI SVLG+T L EG IL+ES ETS F
Sbjct: 361 IAEVAEMDVVEDVKSQDICSVLGET------------------LSEGSSILIESPETSGF 420
Query: 421 VDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHK 480
++T N HKHSD+PHD Q SCRNTR DIGCSCEG+ KQLC E+C +DFQLT AN A E K
Sbjct: 421 INTSTNSHKHSDDPHDCQTSCRNTRKDIGCSCEGHVKQLCREHCSNDFQLTFANGALEQK 480
Query: 481 PFSLGKPNNIDSPEIDCVTR---DDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTIC 540
FSLGKP+NIDSPEID VTR DDDEG M +YTSS S +QQAD DS M FGNG TIC
Sbjct: 481 TFSLGKPSNIDSPEIDIVTRDDYDDDEGTMSIYTSSVSHMIQQADQRDSGMDFGNGRTIC 540
Query: 541 TLGTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRF 600
TLG E+NLA +RKKKMKR RRRRQL +RNEEFQS A TEEYP SIAKYWCQRYQLFSRF
Sbjct: 541 TLGAEKNLAGISRKKKMKRIRRRRQLSERNEEFQSLAITEEYPTSIAKYWCQRYQLFSRF 600
Query: 601 DDGVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
DDGVKMD+EGWFSVTPEPIA+HHALRCG +LIVDSFTGVGGNAIQFSQRAKHVIAIDIDP
Sbjct: 601 DDGVKMDEEGWFSVTPEPIAQHHALRCGCNLIVDSFTGVGGNAIQFSQRAKHVIAIDIDP 660
Query: 661 IKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTM 720
KIRYA HNA+IYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDY KVDTYDLKTM
Sbjct: 661 RKIRYAHHNASIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYTKVDTYDLKTM 720
Query: 721 LKPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIEFC 780
LKPHDGYFLFNIAKKIAPFVVMFLPRNVN+DQLAELSLS+ PPWSLE+E+ L +++
Sbjct: 721 LKPHDGYFLFNIAKKIAPFVVMFLPRNVNVDQLAELSLSSDPPWSLEVEKNFLNGKLKAI 766
Query: 781 IVKGCHIHANMCNGL 793
+ N NG+
Sbjct: 781 TAYFSNGSTNEPNGI 766
BLAST of Sgr027703 vs. ExPASy TrEMBL
Match:
A0A6J1IFL4 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)
HSP 1 Score: 1193.3 bits (3086), Expect = 0.0e+00
Identity = 597/775 (77.03%), Postives = 653/775 (84.26%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG N ESEDE G SAIRA+GSLFKLTEVFLWDDETEVARR E SLA DADD+NNE+F E
Sbjct: 1 MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
KICSTI+DISL PEDI+L EQMN LGLPLSFHTNKE+R GIT GKRK TVKHSRIQ G L
Sbjct: 61 KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKERRTGITMGKRKTTVKHSRIQHGFL 120
Query: 121 DKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDI 180
DKE+EFPK SS+ EIVANIN ND+AIGS+CC+SMV+QSE SDCD V + NESHV FDGDI
Sbjct: 121 DKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDI 180
Query: 181 SPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPR 240
SPNS+GLI GAVEEQSC+V D VLNN DHE G+A+LGDH KV S I LD+ HSPR
Sbjct: 181 SPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHAKVRLSPIGLDKGHSPR 240
Query: 241 RYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDY 300
M G DVSHGKQEEVE PME EGSS TL DTEVQKIDIDSGIGLPLVAE S+L+M DY
Sbjct: 241 ICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGADY 300
Query: 301 NQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKS 360
N+NDHVVGCI + GEW VY DSFYMRNYFYNI T+ESTWNPPPGLEHFA SDAN T N+S
Sbjct: 301 NENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENES 360
Query: 361 IAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAF 420
IAEVAE+DVLED K EDI SVL DT+SCMN GD+IHCQPPDALLEG ILVE +++ A
Sbjct: 361 IAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRAS 420
Query: 421 VDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHK 480
V T IN + DEPH+W SCRNTR I CSCEG+ KQ CHENC + FQL VAN E K
Sbjct: 421 VHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQK 480
Query: 481 PFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTLG 540
FS K +N+DSPE VT DDEGA+GL TSS S LQQADHMD DMHFGN PTICTLG
Sbjct: 481 TFSHCKSSNMDSPEKAFVT-IDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTLG 540
Query: 541 TEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDG 600
TEQNL+ R+RKKKMKRTRRR QL DRNEEF S A TEEYP SI KYWCQRYQLFSRFDDG
Sbjct: 541 TEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDDG 600
Query: 601 VKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
VKMDKEGWFSVTPE IARHHA RCGS++I+DSFTGVGGNAIQFSQRAKHVIAIDIDPIKI
Sbjct: 601 VKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIKI 660
Query: 661 RYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLKP 720
RYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YA+VD YDLKTML+P
Sbjct: 661 RYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRP 720
Query: 721 HDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
HDGYFLFNIAKKIAP VVMFLP+NVNLDQLAE++LS++PPWSLE+E+ L +++
Sbjct: 721 HDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLK 772
BLAST of Sgr027703 vs. ExPASy TrEMBL
Match:
A0A6J1II65 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)
HSP 1 Score: 1188.7 bits (3074), Expect = 0.0e+00
Identity = 597/776 (76.93%), Postives = 653/776 (84.15%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG N ESEDE G SAIRA+GSLFKLTEVFLWDDETEVARR E SLA DADD+NNE+F E
Sbjct: 1 MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKE-KRNGITTGKRKATVKHSRIQQGL 120
KICSTI+DISL PEDI+L EQMN LGLPLSFHTNKE +R GIT GKRK TVKHSRIQ G
Sbjct: 61 KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120
Query: 121 LDKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGD 180
LDKE+EFPK SS+ EIVANIN ND+AIGS+CC+SMV+QSE SDCD V + NESHV FDGD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180
Query: 181 ISPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSP 240
ISPNS+GLI GAVEEQSC+V D VLNN DHE G+A+LGDH KV S I LD+ HSP
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHAKVRLSPIGLDKGHSP 240
Query: 241 RRYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPD 300
R M G DVSHGKQEEVE PME EGSS TL DTEVQKIDIDSGIGLPLVAE S+L+M D
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGAD 300
Query: 301 YNQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANK 360
YN+NDHVVGCI + GEW VY DSFYMRNYFYNI T+ESTWNPPPGLEHFA SDAN T N+
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENE 360
Query: 361 SIAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSA 420
SIAEVAE+DVLED K EDI SVL DT+SCMN GD+IHCQPPDALLEG ILVE +++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRA 420
Query: 421 FVDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEH 480
V T IN + DEPH+W SCRNTR I CSCEG+ KQ CHENC + FQL VAN E
Sbjct: 421 SVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQ 480
Query: 481 KPFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTL 540
K FS K +N+DSPE VT DDEGA+GL TSS S LQQADHMD DMHFGN PTICTL
Sbjct: 481 KTFSHCKSSNMDSPEKAFVT-IDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTL 540
Query: 541 GTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDD 600
GTEQNL+ R+RKKKMKRTRRR QL DRNEEF S A TEEYP SI KYWCQRYQLFSRFDD
Sbjct: 541 GTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDD 600
Query: 601 GVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
GVKMDKEGWFSVTPE IARHHA RCGS++I+DSFTGVGGNAIQFSQRAKHVIAIDIDPIK
Sbjct: 601 GVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
Query: 661 IRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLK 720
IRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YA+VD YDLKTML+
Sbjct: 661 IRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLR 720
Query: 721 PHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
PHDGYFLFNIAKKIAP VVMFLP+NVNLDQLAE++LS++PPWSLE+E+ L +++
Sbjct: 721 PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLK 773
BLAST of Sgr027703 vs. ExPASy TrEMBL
Match:
A0A6J1IEG7 (Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=1)
HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 595/765 (77.78%), Postives = 646/765 (84.44%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG N ESEDE G SAIRA+GSLFKLTEVFLWDDETEVARR E SLA DADD+NNE+F E
Sbjct: 1 MGSSNEESEDEAGVSAIRAIGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFRE 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKE-KRNGITTGKRKATVKHSRIQQGL 120
KICSTI+DISL PEDI+L EQMN LGLPLSFHTNKE +R GIT GKRK TVKHSRIQ G
Sbjct: 61 KICSTITDISLSPEDIQLTEQMNALGLPLSFHTNKEQRRTGITMGKRKTTVKHSRIQHGF 120
Query: 121 LDKEMEFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGD 180
LDKE+EFPK SS+ EIVANIN ND+AIGS+CC+SMV+QSE SDCD V + NESHV FDGD
Sbjct: 121 LDKEVEFPKFSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGD 180
Query: 181 ISPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSP 240
ISPNS+GLI GAVEEQSC+V D VLNN DHE G+A+LGDH KV S I LD+ HSP
Sbjct: 181 ISPNSSGLIHGAVEEQSCNVKCDIVLNNRGDHE--SGDALLGDHAKVRLSPIGLDKGHSP 240
Query: 241 RRYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPD 300
R M G DVSHGKQEEVE PME EGSS TL DTEVQKIDIDSGIGLPLVAE S+L+M D
Sbjct: 241 RICMTGFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSYLHMGAD 300
Query: 301 YNQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANK 360
YN+NDHVVGCI + GEW VY DSFYMRNYFYNI T+ESTWNPPPGLEHFA SDAN T N+
Sbjct: 301 YNENDHVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPPGLEHFAHSDANFTENE 360
Query: 361 SIAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSA 420
SIAEVAE+DVLED K EDI SVL DT+SCMN GD+IHCQPPDALLEG ILVE +++ A
Sbjct: 361 SIAEVAEMDVLEDAKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKSRA 420
Query: 421 FVDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEH 480
V T IN + DEPH+W SCRNTR I CSCEG+ KQ CHENC + FQL VAN E
Sbjct: 421 SVHTSINSYMQPDEPHEWLTSCRNTREIIECSCEGHVKQPCHENCSNGFQLIVANETSEQ 480
Query: 481 KPFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTL 540
K FS K +N+DSPE VT DDEGA+GL TSS S LQQADHMD DMHFGN PTICTL
Sbjct: 481 KTFSHCKSSNMDSPEKAFVT-IDDEGAVGLTTSSVSHVLQQADHMDGDMHFGNEPTICTL 540
Query: 541 GTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDD 600
GTEQNL+ R+RKKKMKRTRRR QL DRNEEF S A TEEYP SI KYWCQRYQLFSRFDD
Sbjct: 541 GTEQNLSGRDRKKKMKRTRRRGQLSDRNEEFHSLAITEEYPTSITKYWCQRYQLFSRFDD 600
Query: 601 GVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
GVKMDKEGWFSVTPE IARHHA RCGS++I+DSFTGVGGNAIQFSQRAKHVIAIDIDPIK
Sbjct: 601 GVKMDKEGWFSVTPESIARHHASRCGSNMIIDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
Query: 661 IRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLK 720
IRYAQHNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YA+VD YDLKTML+
Sbjct: 661 IRYAQHNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLR 720
Query: 721 PHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLE 765
PHDGYFLFNIAKKIAP VVMFLP+NVNLDQLAE++LS++PPWSLE
Sbjct: 721 PHDGYFLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLE 762
BLAST of Sgr027703 vs. ExPASy TrEMBL
Match:
A0A6J1FDE5 (Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 SV=1)
HSP 1 Score: 1173.3 bits (3034), Expect = 0.0e+00
Identity = 590/771 (76.52%), Postives = 645/771 (83.66%), Query Frame = 0
Query: 5 NGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGEKICS 64
+G E E G SAIRALGSLFKLTEVFLWDDETEVARR E SLA DADD+NNE+F EKICS
Sbjct: 3 SGNEESEAGVSAIRALGSLFKLTEVFLWDDETEVARRVESSLALDADDANNEKFREKICS 62
Query: 65 TISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLLDKEM 124
TI+DISL PEDIEL EQMN LGLPLSFHTNKE+R GIT GKR TVKHSRIQQG LDKE+
Sbjct: 63 TITDISLSPEDIELTEQMNALGLPLSFHTNKERRTGITMGKRNTTVKHSRIQQGFLDKEV 122
Query: 125 EFPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGDISPNS 184
EFPK+SS+ EIVANIN ND+AIGS+CC+SMV+QSE SDCD V + NESHV FDGDISPNS
Sbjct: 123 EFPKSSSRGEIVANINLNDEAIGSLCCSSMVNQSEASDCDAVFEANESHVIFDGDISPNS 182
Query: 185 TGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSPRRYMI 244
+GLI GA EEQSC V D VLNN DHE G+AVLGDH KV SSI LD+ HSPR M
Sbjct: 183 SGLIHGAFEEQSCDVTCDIVLNNRGDHE--SGDAVLGDHAKVRLSSIGLDKGHSPRICMT 242
Query: 245 GLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPDYNQND 304
G DVSHGKQEEVE PME EGSS TL DTEVQKIDIDSGIGLPLVAE SFL+M DYN+ND
Sbjct: 243 GFDVSHGKQEEVELPMELEGSSTTLQDTEVQKIDIDSGIGLPLVAEQSFLHMGADYNEND 302
Query: 305 HVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANKSIAEV 364
HVVGCI + GEW VY DSFYMRNYFYNI T+ESTWNPP GLEHFA DAN T N+SIAEV
Sbjct: 303 HVVGCIQEYGEWTVYWDSFYMRNYFYNIKTHESTWNPPLGLEHFAHFDANFTENESIAEV 362
Query: 365 AEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSAFVDTL 424
AE+DVLED+K EDI SVL DT+SCMN GD+IHCQPPDALLEG ILVE ++ A V+T
Sbjct: 363 AEMDVLEDLKPEDICSVLVDTRSCMNLPGDNIHCQPPDALLEGSSILVEGSKNRASVNTS 422
Query: 425 INVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEHKPFSL 484
IN + DEPH+W + RNTR I CSCEG+ KQ CHENC + FQL VAN A E K FS
Sbjct: 423 INSYMQPDEPHEWLTNRRNTREIIECSCEGHVKQPCHENCSNGFQLIVANEASEQKTFSH 482
Query: 485 GKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTLGTEQN 544
KP+N+ SPE VT DDEGA+ L TSS S LQQADHM+ DMHFGN PTICTLGTEQN
Sbjct: 483 CKPSNMYSPEKAFVT-IDDEGAVDLTTSSVSHVLQQADHMNGDMHFGNEPTICTLGTEQN 542
Query: 545 LAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDDGVKMD 604
L+ R+RKKKMKRTRRR Q DRNEEF S A TEEYP SI KYWCQRYQLFSRFDDGVKMD
Sbjct: 543 LSGRDRKKKMKRTRRRGQFSDRNEEFHSPAITEEYPTSITKYWCQRYQLFSRFDDGVKMD 602
Query: 605 KEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQ 664
KEGWFSVTPE IARHHA RCGS++IVDSFTGVGGNAIQFSQRAKHVIAIDIDP KIRYAQ
Sbjct: 603 KEGWFSVTPESIARHHASRCGSNMIVDSFTGVGGNAIQFSQRAKHVIAIDIDPTKIRYAQ 662
Query: 665 HNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLKPHDGY 724
HNAA+YGVEDQIDFIKGDFFRLAP LKADVIFLSPPWGGP+YA+VD YDLKTML+PHDGY
Sbjct: 663 HNAALYGVEDQIDFIKGDFFRLAPRLKADVIFLSPPWGGPNYARVDIYDLKTMLRPHDGY 722
Query: 725 FLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
FLFNIAKKIAP VVMFLP+NVNLDQLAE++LS++PPWSLE+E+ L +++
Sbjct: 723 FLFNIAKKIAPVVVMFLPKNVNLDQLAEMALSSNPPWSLEVEKNFLNGKLK 770
BLAST of Sgr027703 vs. TAIR 10
Match:
AT1G45231.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 330.5 bits (846), Expect = 4.0e-90
Identity = 253/776 (32.60%), Postives = 349/776 (44.97%), Query Frame = 0
Query: 1 MGFCNGESEDEVGASAIRALGSLFKLTEVFLWDDETEVARRAERSLAPDADDSNNEEFGE 60
MG GE E+ G AI ALGSLFKLT++ LW D T+ + S DD+ +G
Sbjct: 1 MGKVRGEVEEAEG-EAIEALGSLFKLTQIHLWVD-TDTPLFHQHSGNKSVDDT----YG- 60
Query: 61 KICSTISDISLLPEDIELNEQMNDLGLPLSFHTNKEKRNGITTGKRKATVKHSRIQQGLL 120
I++ L+ E MNDLGLP+SF TNK+ +N T G +K K L
Sbjct: 61 -----IANNGLMKE-------MNDLGLPVSFRTNKQWKNR-TRGYQKKGFKDR------L 120
Query: 121 DKEME-FPKASSKEEIVANINFNDDAIGSICCTSMVDQSETSDCDVVLDVNESHVCFDGD 180
D+E+ K +V+ +N + + S C DC V++V E
Sbjct: 121 DEEVNVILKVEDTLNLVSALN---EEVESPCF--------EEDCVQVIEVEE-------- 180
Query: 181 ISPNSTGLISGAVEEQSCSVMFDNVLNNGEDHELSLGNAVLGDHTKVTSSSISLDQDHSP 240
E+HE+ +G+ LG+
Sbjct: 181 -----------------------------ENHEVVVGSCFLGN----------------- 240
Query: 241 RRYMIGLDVSHGKQEEVEPPMESEGSSITLHDTEVQKIDIDSGIGLPLVAESSFLYMEPD 300
+G S+ +T IDS
Sbjct: 241 ----------------------GDGDSVLASET------IDS------------------ 300
Query: 301 YNQNDHVVGCIHKSGEWMVYLDSFYMRNYFYNINTYESTWNPPPGLEHFALSDANCTANK 360
H S W VY DSFY R+YFYN T ES W PP G+EH A SD + ++
Sbjct: 301 -----------HDSSVWKVYWDSFYGRSYFYNFKTQESKWEPPLGMEHLAYSDESHNLSE 360
Query: 361 SIAEVAEVDVLEDVKSEDITSVLGDTQSCMNFLGDHIHCQPPDALLEGPGILVESTETSA 420
+ E D+ V +D+ D LG Q + E+TE
Sbjct: 361 LVIEKHHDDLSGTVLGDDVPFDKADD------LGGVCQSQ----------LEAEATEE-- 420
Query: 421 FVDTLINVHKHSDEPHDWQLSCRNTRADIGCSCEGYAKQLCHENCHSDFQLTVANRAFEH 480
V++LI+ ++ + ++ N++ +
Sbjct: 421 -VNSLIDTYQ---------------------------------------ETSIGNQSLD- 480
Query: 481 KPFSLGKPNNIDSPEIDCVTRDDDEGAMGLYTSSASRKLQQADHMDSDMHFGNGPTICTL 540
+T +EG G Y S
Sbjct: 481 ------------------ITSLGEEGT-GAYVVS-------------------------- 518
Query: 541 GTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQATTEEYPASIAKYWCQRYQLFSRFDD 600
+VR KK+ +R+R +++L + + + EEY + KYWCQRY LFSRFD+
Sbjct: 541 ------SVRKAKKESRRSRAKKKLLNSYTGTEMKGVPEEYSPILGKYWCQRYLLFSRFDE 518
Query: 601 GVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQFSQRAKHVIAIDIDPIK 660
G+KMD+EGWFSVTPE IA+HHA RC +++D FTGVGGNAIQF+ R+ +VIAID+DP K
Sbjct: 601 GIKMDEEGWFSVTPELIAKHHATRCNEGIVIDCFTGVGGNAIQFASRSHYVIAIDLDPKK 518
Query: 661 IRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWGGPDYAKVDTYDLKTMLK 720
+ A+HNAAIYGV D+IDF+KGDFF LA +LKA +FLSPPWGGPDY K TYD+KTML+
Sbjct: 661 LDLAKHNAAIYGVADKIDFVKGDFFDLAHNLKAGTVFLSPPWGGPDYLKASTYDMKTMLR 518
Query: 721 PHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWSLELEQFLLQVEIE 776
P DG LF A IA ++MFLPRNV+++QLAEL+LS PPWSLE+E+ L +++
Sbjct: 721 PRDGDALFKAAMNIASTIIMFLPRNVDINQLAELALSTSPPWSLEVEKNYLNGKLK 518
BLAST of Sgr027703 vs. TAIR 10
Match:
AT1G30550.2 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 237.7 bits (605), Expect = 3.5e-62
Identity = 108/193 (55.96%), Postives = 145/193 (75.13%), Query Frame = 0
Query: 583 IAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSFTGVGGNAIQ 642
I++YW QRY LFS++D G++MD+EGW+SVTPE IA A RC +++D F+GVGGN IQ
Sbjct: 19 ISRYWIQRYDLFSKYDQGIEMDEEGWYSVTPEEIAIKQAERCRGKVVIDCFSGVGGNTIQ 78
Query: 643 FSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKADVIFLSPPWG 702
F++ VIAIDIDP+KI A +NA +YGV ++IDF+ GDF +LAPSLK DV+FLSPPWG
Sbjct: 79 FAKVCSSVIAIDIDPMKIALAMNNAKVYGVANRIDFVTGDFMQLAPSLKGDVLFLSPPWG 138
Query: 703 GPDYAKVDTYDLKTMLKPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAELSLSAHPPWS 762
GP Y+KV++Y L ML P DGY LF A I P ++MFLP+N++L QL EL+ + PP +
Sbjct: 139 GPTYSKVESYKL-DMLLPRDGYSLFQTALSITPNIIMFLPKNIDLAQLEELACLSSPPLT 198
Query: 763 LELEQFLLQVEIE 776
LE+E+ + EI+
Sbjct: 199 LEIEENSIGGEIK 210
BLAST of Sgr027703 vs. TAIR 10
Match:
AT1G30550.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )
HSP 1 Score: 229.9 bits (585), Expect = 7.3e-60
Identity = 117/254 (46.06%), Postives = 167/254 (65.75%), Query Frame = 0
Query: 514 ASRKLQQADHMDSDMHFGNGPTICTLGTEQNLAVRNRKKKMKRTRRRRQLFDRNEEFQSQ 573
+SR ++ + +DS H E + R+ K K KR+R ++++ E+ +
Sbjct: 203 SSRTIRGVERVDSLTH------------ESHNMTRSMKLK-KRSRLKKEVKSTIEKDNGR 262
Query: 574 ATTEEYPASIAKYWCQRYQLFSRFDDGVKMDKEGWFSVTPEPIARHHALRCGSSLIVDSF 633
I KYW QRY LFSR+D G++MD+EGW+SVTPE IA A R +++D F
Sbjct: 263 R------HKITKYWIQRYDLFSRYDQGIEMDEEGWYSVTPEEIAIKQAQRYRGKVVIDCF 322
Query: 634 TGVGGNAIQFSQRAKHVIAIDIDPIKIRYAQHNAAIYGVEDQIDFIKGDFFRLAPSLKAD 693
+GVGGN IQF++ V+AIDIDP+K+ A +NA +YGV +++DF+ GDF +LAPSLK D
Sbjct: 323 SGVGGNTIQFAKVCSSVVAIDIDPVKVELAMNNAMVYGVANRVDFVIGDFIQLAPSLKGD 382
Query: 694 VIFLSPPWGGPDYAKVDTYDLKTMLKPHDGYFLFNIAKKIAPFVVMFLPRNVNLDQLAEL 753
V+FLSPPWGGP Y ++Y+L ML+P DGY LF IA+ I P ++MFLPRNV+L Q+ EL
Sbjct: 383 VVFLSPPWGGPMYRDFESYNL-DMLQPRDGYSLFQIAQSITPNIIMFLPRNVDLAQVEEL 436
Query: 754 SLSAHPPWSLELEQ 768
+ + PP +LE+E+
Sbjct: 443 AWLSSPPLNLEIEE 436
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022138324.1 | 0.0e+00 | 76.48 | uncharacterized protein LOC111009535 isoform X1 [Momordica charantia] >XP_022138... | [more] |
| XP_038897817.1 | 0.0e+00 | 77.29 | uncharacterized protein LOC120085727 isoform X2 [Benincasa hispida] | [more] |
| XP_038897816.1 | 0.0e+00 | 77.19 | uncharacterized protein LOC120085727 isoform X1 [Benincasa hispida] | [more] |
| XP_022975956.1 | 0.0e+00 | 77.03 | uncharacterized protein LOC111476503 isoform X2 [Cucurbita maxima] | [more] |
| XP_023535337.1 | 0.0e+00 | 77.16 | uncharacterized protein LOC111796805 isoform X2 [Cucurbita pepo subsp. pepo] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| P85107 | 1.7e-61 | 50.84 | Trimethylguanosine synthase OS=Rattus norvegicus OX=10116 GN=Tgs1 PE=1 SV=1 | [more] |
| Q96RS0 | 9.3e-60 | 56.35 | Trimethylguanosine synthase OS=Homo sapiens OX=9606 GN=TGS1 PE=1 SV=3 | [more] |
| Q923W1 | 2.7e-59 | 54.07 | Trimethylguanosine synthase OS=Mus musculus OX=10090 GN=Tgs1 PE=1 SV=2 | [more] |
| Q09814 | 2.1e-35 | 39.15 | Trimethylguanosine synthase OS=Schizosaccharomyces pombe (strain 972 / ATCC 2484... | [more] |
| Q12052 | 4.6e-27 | 34.82 | Trimethylguanosine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1C9F8 | 0.0e+00 | 76.48 | Trimethylguanosine synthase OS=Momordica charantia OX=3673 GN=LOC111009535 PE=4 ... | [more] |
| A0A6J1IFL4 | 0.0e+00 | 77.03 | Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... | [more] |
| A0A6J1II65 | 0.0e+00 | 76.93 | Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... | [more] |
| A0A6J1IEG7 | 0.0e+00 | 77.78 | Trimethylguanosine synthase OS=Cucurbita maxima OX=3661 GN=LOC111476503 PE=4 SV=... | [more] |
| A0A6J1FDE5 | 0.0e+00 | 76.52 | Trimethylguanosine synthase OS=Cucurbita moschata OX=3662 GN=LOC111443000 PE=4 S... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| AT1G45231.2 | 4.0e-90 | 32.60 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | [more] |
| AT1G30550.2 | 3.5e-62 | 55.96 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | [more] |
| AT1G30550.1 | 7.3e-60 | 46.06 | S-adenosyl-L-methionine-dependent methyltransferases superfamily protein | [more] |
