Sgr027650 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr027650
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Calcium-binding EF-hand family protein
Location	tig00153055: 1477331 .. 1477906 (+)
RNA-Seq Expression	Sgr027650
Synteny	Sgr027650

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATTCCAGCCGTTTCCATTACTTTCAAATGGTTTTCGAAAACCAATTTCAAGCTGATCAACTTCCCTCGCCTTCTTTCCAAATCCAAACCACCGTCGTCGGAACCTTCTCTTCCACCCCTCACGGCGGCGCTTCCCCAGAGAAAAATCTCCACCTCGCAACCACTCGCGGAAGCTGCCTTTCGTCACCTCGACGCCGACGGAGACGGCAAAATCTCCGGCGAAGAGCTCCGGTCCTACTTCGCATCCATCGGCGAGTCCCTGACGCGCGACGGTGCCCGGAGAGTGATCAGGGACTTCGACGCCGACGGCGATGAGCTGCTGGAGCTTCAAGATTTCGTGCGGCTGGTGGAGGCAGAGAACGAGGAAGAGGAGGATTTGAGAAGAGCTTTTGAGATGTTTGAAGTTGAAAAGGGCTGCGGGTTCATCGGACCGGCGGGATTGCAGAAGATGTTTAGCCGGCTTGGGTACGTGAAATCAAACGACGAGTGCATGGCTATGATCGGCGCCTTCGATCTCGACGGCGACGGCGTGATCGATTATCGTGAATTTCTCAGAATGATGGCGGCGTAA

mRNA sequence

ATGGCGATTCCAGCCGTTTCCATTACTTTCAAATGGTTTTCGAAAACCAATTTCAAGCTGATCAACTTCCCTCGCCTTCTTTCCAAATCCAAACCACCGTCGTCGGAACCTTCTCTTCCACCCCTCACGGCGGCGCTTCCCCAGAGAAAAATCTCCACCTCGCAACCACTCGCGGAAGCTGCCTTTCGTCACCTCGACGCCGACGGAGACGGCAAAATCTCCGGCGAAGAGCTCCGGTCCTACTTCGCATCCATCGGCGAGTCCCTGACGCGCGACGGTGCCCGGAGAGTGATCAGGGACTTCGACGCCGACGGCGATGAGCTGCTGGAGCTTCAAGATTTCGTGCGGCTGGTGGAGGCAGAGAACGAGGAAGAGGAGGATTTGAGAAGAGCTTTTGAGATGTTTGAAGTTGAAAAGGGCTGCGGGTTCATCGGACCGGCGGGATTGCAGAAGATGTTTAGCCGGCTTGGGTACGTGAAATCAAACGACGAGTGCATGGCTATGATCGGCGCCTTCGATCTCGACGGCGACGGCGTGATCGATTATCGTGAATTTCTCAGAATGATGGCGGCGTAA

Coding sequence (CDS)

ATGGCGATTCCAGCCGTTTCCATTACTTTCAAATGGTTTTCGAAAACCAATTTCAAGCTGATCAACTTCCCTCGCCTTCTTTCCAAATCCAAACCACCGTCGTCGGAACCTTCTCTTCCACCCCTCACGGCGGCGCTTCCCCAGAGAAAAATCTCCACCTCGCAACCACTCGCGGAAGCTGCCTTTCGTCACCTCGACGCCGACGGAGACGGCAAAATCTCCGGCGAAGAGCTCCGGTCCTACTTCGCATCCATCGGCGAGTCCCTGACGCGCGACGGTGCCCGGAGAGTGATCAGGGACTTCGACGCCGACGGCGATGAGCTGCTGGAGCTTCAAGATTTCGTGCGGCTGGTGGAGGCAGAGAACGAGGAAGAGGAGGATTTGAGAAGAGCTTTTGAGATGTTTGAAGTTGAAAAGGGCTGCGGGTTCATCGGACCGGCGGGATTGCAGAAGATGTTTAGCCGGCTTGGGTACGTGAAATCAAACGACGAGTGCATGGCTATGATCGGCGCCTTCGATCTCGACGGCGACGGCGTGATCGATTATCGTGAATTTCTCAGAATGATGGCGGCGTAA

Protein sequence

MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVEAENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGVIDYREFLRMMAA

Homology

[Close]

BLAST of Sgr027650 vs. NCBI nr
Match: XP_022139654.1 (probable calcium-binding protein CML41 [Momordica charantia])

HSP 1 Score: 271.2 bits (692), Expect = 7.0e-69
Identity = 144/189 (76.19%), Postives = 153/189 (80.95%), Query Frame = 0


Query: 3   IPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEAAF 62
           IP VSI FKWFSK+NFKL NFPRLL   KP SS P  PPL AA          PLAE AF
Sbjct: 5   IPTVSIAFKWFSKSNFKL-NFPRLLFNPKPTSSNPPPPPLKAA---------PPLAEVAF 64

Query: 63  RHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVEAEN 122
            HLDADGDGKIS +ELRSYFASIGE +TRD  RRVIRDFD +GDELLELQDF RL+E + 
Sbjct: 65  HHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQE 124

Query: 123 EEE-EDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGVID 182
           EEE EDLRRAFEMFEVEKGCGFIGP GLQKMF RLGYVKS+DECMAMI  FD++GDGVID
Sbjct: 125 EEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVID 183

Query: 183 YREFLRMMA 191
           YREFLRMMA
Sbjct: 185 YREFLRMMA 183

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BLAST of Sgr027650 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.4 bits (651), Expect = 4.0e-64
Identity = 137/190 (72.11%), Postives = 150/190 (78.95%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MA+  VS  FKWFS TNF L NFPRL  K KP  S P  PPL    P      + PLAEA
Sbjct: 38  MALSPVSTPFKWFSNTNFNL-NFPRLRFKHKPTRSNPPPPPLLLLTP------APPLAEA 97

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVE- 120
           AFRHLDADGDGKISG+EL+SYFAS+GE +TRD AR +IR+FD DGD LLEL DF RLV+ 
Sbjct: 98  AFRHLDADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKG 157

Query: 121 AENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
            E EE+EDL+RAFEMFE EKGCGFIGPAGLQKMF RLGYVKS +ECMAMIG FD+DGDGV
Sbjct: 158 GEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGV 217

Query: 181 IDYREFLRMM 190
           IDYREFLRMM
Sbjct: 218 IDYREFLRMM 220

[Close]

BLAST of Sgr027650 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 254.2 bits (648), Expect = 8.8e-64
Identity = 135/190 (71.05%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MA+  +S  FKWFS TN KL N PRL  K KP  S P  PPL    P      + PLAEA
Sbjct: 1   MALSPISTPFKWFSNTNLKL-NLPRLRFKHKPTRSNPPPPPLLLLTP------APPLAEA 60

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVE- 120
           AFRHLDADGDGKISG+EL+SYFAS+GE +TRD AR +IR+FD DGD LLEL DF RLV+ 
Sbjct: 61  AFRHLDADGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKG 120

Query: 121 AENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
            E EE+EDL+RAFEMFE EKGCGFIGPAGLQKMF RLGYVKS +ECMAMIG FD+DGDGV
Sbjct: 121 GEEEEKEDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGV 180

Query: 181 IDYREFLRMM 190
           IDYREFLRMM
Sbjct: 181 IDYREFLRMM 183

[Close]

BLAST of Sgr027650 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 253.8 bits (647), Expect = 1.2e-63
Identity = 135/190 (71.05%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MA+  +S  FKWFS TNFKL NFPRL  K KP  S P  PPL    P      + PLAEA
Sbjct: 15  MALSPISTPFKWFSNTNFKL-NFPRLRFKHKPTPSNPPPPPLLLLTP------APPLAEA 74

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVE- 120
           AFRHLDADGDGKISG+EL+SYFAS+GE +TRD AR +IR+FD DGD LLEL DF RLV+ 
Sbjct: 75  AFRHLDADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG 134

Query: 121 AENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
            E EE+EDL+ AFEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMIG FD+DGDGV
Sbjct: 135 GEEEEKEDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGV 194

Query: 181 IDYREFLRMM 190
           IDYREFLRMM
Sbjct: 195 IDYREFLRMM 197

[Close]

BLAST of Sgr027650 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 253.4 bits (646), Expect = 1.5e-63
Identity = 135/190 (71.05%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MA+  +S  FKWFS TN KL N PRL  K KP  S P  PPL    P      + PLAEA
Sbjct: 1   MALSPISTPFKWFSNTNLKL-NLPRLRFKHKPTRSNPPPPPLLLLTP------APPLAEA 60

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVE- 120
           AFRHLDADGDGKISG+EL+SYFAS+GE +TRD AR +IR+FD DGD LLEL DF RLV+ 
Sbjct: 61  AFRHLDADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG 120

Query: 121 AENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
            E EE+EDL+RAFEMFE EKGCGFIGPAGLQKMF RLGYVKS +ECMAMIG FD+DGDGV
Sbjct: 121 GEEEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGV 180

Query: 181 IDYREFLRMM 190
           IDYREFLRMM
Sbjct: 181 IDYREFLRMM 183

[Close]

BLAST of Sgr027650 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 3.9e-30
Identity = 80/194 (41.24%), Postives = 110/194 (56.70%), Query Frame = 0


Query: 7   SITFKWFSKTNFKL-INFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAE---AAF 66
           S +FKWFS    KL ++F       K  SS     P + +     I + Q   E     F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 67  RHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV---- 126
            H D+DGDGKIS  ELR YF S+GE ++ + A+  I + D D D  L  +DFV L+    
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 127 ---EAENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLD 186
              + E + + +L+ AFEMFEVEKG G I P GLQKM  +LG  ++  EC AMI  +D+D
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

Query: 187 GDGVIDYREFLRMM 190
           G+G++D+ EF +MM
Sbjct: 190 GNGILDFHEFRQMM 203

[Close]

BLAST of Sgr027650 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.5e-21
Identity = 65/141 (46.10%), Postives = 86/141 (60.99%), Query Frame = 0


Query: 58  AEAAFRHLDADGDGKISGEELRSYF-ASIGESLTRDGARRVIRDFDADGDELLELQDFVR 117
           + A F   D DGDG+IS  ELR     ++GE ++ + A +++   DADGD LL   +FVR
Sbjct: 12  SSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCEAEFVR 71

Query: 118 LVEAENEEEED------LRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIG 177
           LV+A   EEED      LR AF M+E+E G G I P  L++M  RLG  +  D+C AMI 
Sbjct: 72  LVQAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDCRAMIC 131

Query: 178 AFDLDGDGVIDYREFLRMMAA 192
            FDL+GDGV+ + EF  MM A
Sbjct: 132 RFDLNGDGVLSFDEFKIMMNA 151

[Close]

BLAST of Sgr027650 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.3e-21
Identity = 53/133 (39.85%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 59  EAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV 118
           EA F ++DA+ DG+IS EEL+  F ++GE L+ + A   +R  D DGD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 119 EAENEEEE--DLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDG 178
           + ++EEE+  +L+ AF ++ + +G   I P  L+ M  +LG  ++ D+C  MI AFDL+ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 179 DGVIDYREFLRMM 190
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Sgr027650 vs. ExPASy Swiss-Prot
Match: Q8RYJ9 (Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 GN=CML23 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.8e-20
Identity = 65/142 (45.77%), Postives = 84/142 (59.15%), Query Frame = 0


Query: 62  FRHLDADGDGKISGEELR-SYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV-- 121
           F   D DGDGKIS  ELR    AS+GE +  +  + ++   D DGD LL+ ++FVRLV  
Sbjct: 11  FGSFDQDGDGKISATELRLCVKASLGEDMPDEEVQALMALADTDGDGLLDEEEFVRLVTE 70

Query: 122 -EAENEEEED--------LRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMI 181
            EA+ +EEED        LR AF M+E+E G G I P  L+ M S+LG      EC AMI
Sbjct: 71  MEADGDEEEDDDDETCRCLREAFAMYEME-GRGCITPLSLKLMLSKLGTHLDVAECQAMI 130

Query: 182 GAFDLDGDGVIDYREFLRMMAA 192
             FD++GDGV+ + EF  MM A
Sbjct: 131 CRFDMNGDGVLTFDEFKTMMMA 151

[Close]

BLAST of Sgr027650 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.1e-19
Identity = 52/135 (38.52%), Postives = 81/135 (60.00%), Query Frame = 0


Query: 59  EAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV 118
           EA F ++DA+ DG+IS EEL+  F ++GE ++ + A   ++  D DGD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 119 EAENE--EEEDLRRAFEMFE--VEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDL 178
           +  +E  EEE  R+  E F   +  G   I P  L+ M  +LG  ++ D+C  MI AFDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 179 DGDGVIDYREFLRMM 190
           + DGV+ + EF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of Sgr027650 vs. ExPASy TrEMBL
Match: A0A6J1CDM6 (probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC111010501 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 3.4e-69
Identity = 144/189 (76.19%), Postives = 153/189 (80.95%), Query Frame = 0


Query: 3   IPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEAAF 62
           IP VSI FKWFSK+NFKL NFPRLL   KP SS P  PPL AA          PLAE AF
Sbjct: 5   IPTVSIAFKWFSKSNFKL-NFPRLLFNPKPTSSNPPPPPLKAA---------PPLAEVAF 64

Query: 63  RHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVEAEN 122
            HLDADGDGKIS +ELRSYFASIGE +TRD  RRVIRDFD +GDELLELQDF RL+E + 
Sbjct: 65  HHLDADGDGKISCDELRSYFASIGECITRDDVRRVIRDFDGNGDELLELQDFARLMEVQE 124

Query: 123 EEE-EDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGVID 182
           EEE EDLRRAFEMFEVEKGCGFIGP GLQKMF RLGYVKS+DECMAMI  FD++GDGVID
Sbjct: 125 EEEGEDLRRAFEMFEVEKGCGFIGPEGLQKMFRRLGYVKSSDECMAMIDVFDINGDGVID 183

Query: 183 YREFLRMMA 191
           YREFLRMMA
Sbjct: 185 YREFLRMMA 183

[Close]

BLAST of Sgr027650 vs. ExPASy TrEMBL
Match: A0A6J1IIY2 (probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC111473738 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 5.6e-64
Identity = 135/190 (71.05%), Postives = 149/190 (78.42%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MA+  +S  FKWFS TNFKL NFPRL  K KP  S P  PPL    P      + PLAEA
Sbjct: 15  MALSPISTPFKWFSNTNFKL-NFPRLRFKHKPTPSNPPPPPLLLLTP------APPLAEA 74

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVE- 120
           AFRHLDADGDGKISG+EL+SYFAS+GE +TRD AR +IR+FD DGD LLEL DF RLV+ 
Sbjct: 75  AFRHLDADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG 134

Query: 121 AENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
            E EE+EDL+ AFEMFE EKGCGFIGP GLQKMF RLGYVKS +ECMAMIG FD+DGDGV
Sbjct: 135 GEEEEKEDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGV 194

Query: 181 IDYREFLRMM 190
           IDYREFLRMM
Sbjct: 195 IDYREFLRMM 197

[Close]

BLAST of Sgr027650 vs. ExPASy TrEMBL
Match: A0A6J1F7Y7 (probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC111442970 PE=4 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 2.8e-63
Identity = 133/190 (70.00%), Postives = 150/190 (78.95%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MA+  +S  FKWFS TN KL N PRL  K KP  S P  PPL        ++ + PLAEA
Sbjct: 1   MALSPISTPFKWFSNTNLKL-NLPRLRFKHKPTRSNPPPPPLL-------LTPAPPLAEA 60

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVE- 120
           AFRHLDADGDGKISG+EL+SYFAS+GE +TRD AR +IR+FD DGD LLEL DF RLV+ 
Sbjct: 61  AFRHLDADGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG 120

Query: 121 AENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
            E EE+EDL+RAFEMFE EKGCGFIGPAGLQKMF RLGYVKS +ECMAMIG FD+DG+GV
Sbjct: 121 GEQEEKEDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGNGV 180

Query: 181 IDYREFLRMM 190
           IDYREFLRMM
Sbjct: 181 IDYREFLRMM 182

[Close]

BLAST of Sgr027650 vs. ExPASy TrEMBL
Match: A0A5A7UHX6 (Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold27333G00010 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 6.8e-62
Identity = 133/190 (70.00%), Postives = 145/190 (76.32%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MAI  ++I FKWF+KTNFKL NFP L SK KP SS+P  PP              PLAE 
Sbjct: 1   MAISTIAIPFKWFTKTNFKL-NFPLLHSKHKPISSKPPTPP--------------PLAEV 60

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVEA 120
           AFRHLD DGDGKISG+ELRSYFASIGE +T DGA+ VI DFD DGDELLEL DF RLV+ 
Sbjct: 61  AFRHLDIDGDGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKG 120

Query: 121 ENEE-EEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
           E EE EEDL+RAFEMFE EKGCGFIG  GLQKMF RLGYVKS ++CM MI  FD+DGDGV
Sbjct: 121 EEEEQEEDLKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGV 175

Query: 181 IDYREFLRMM 190
           IDY EFLRMM
Sbjct: 181 IDYHEFLRMM 175

[Close]

BLAST of Sgr027650 vs. ExPASy TrEMBL
Match: A0A1S3CT00 (probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 PE=4 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 6.8e-62
Identity = 133/190 (70.00%), Postives = 145/190 (76.32%), Query Frame = 0


Query: 1   MAIPAVSITFKWFSKTNFKLINFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAEA 60
           MAI  ++I FKWF+KTNFKL NFP L SK KP SS+P  PP              PLAE 
Sbjct: 1   MAISTIAIPFKWFTKTNFKL-NFPLLHSKHKPISSKPPTPP--------------PLAEV 60

Query: 61  AFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLVEA 120
           AFRHLD DGDGKISG+ELRSYFASIGE +T DGA+ VI DFD DGDELLEL DF RLV+ 
Sbjct: 61  AFRHLDIDGDGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKG 120

Query: 121 ENEE-EEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDGDGV 180
           E EE EEDL+RAFEMFE EKGCGFIG  GLQKMF RLGYVKS ++CM MI  FD+DGDGV
Sbjct: 121 EEEEQEEDLKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGV 175

Query: 181 IDYREFLRMM 190
           IDY EFLRMM
Sbjct: 181 IDYHEFLRMM 175

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BLAST of Sgr027650 vs. TAIR 10
Match: AT3G50770.1 (calmodulin-like 41 )

HSP 1 Score: 132.9 bits (333), Expect = 2.8e-31
Identity = 80/194 (41.24%), Postives = 110/194 (56.70%), Query Frame = 0


Query: 7   SITFKWFSKTNFKL-INFPRLLSKSKPPSSEPSLPPLTAALPQRKISTSQPLAE---AAF 66
           S +FKWFS    KL ++F       K  SS     P + +     I + Q   E     F
Sbjct: 10  SNSFKWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVF 69

Query: 67  RHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV---- 126
            H D+DGDGKIS  ELR YF S+GE ++ + A+  I + D D D  L  +DFV L+    
Sbjct: 70  SHFDSDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRD 129

Query: 127 ---EAENEEEEDLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLD 186
              + E + + +L+ AFEMFEVEKG G I P GLQKM  +LG  ++  EC AMI  +D+D
Sbjct: 130 LYGDGEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDID 189

Query: 187 GDGVIDYREFLRMM 190
           G+G++D+ EF +MM
Sbjct: 190 GNGILDFHEFRQMM 203

[Close]

BLAST of Sgr027650 vs. TAIR 10
Match: AT1G76650.1 (calmodulin-like 38 )

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-22
Identity = 53/133 (39.85%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 59  EAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV 118
           EA F ++DA+ DG+IS EEL+  F ++GE L+ + A   +R  D DGD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 119 EAENEEEE--DLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDG 178
           + ++EEE+  +L+ AF ++ + +G   I P  L+ M  +LG  ++ D+C  MI AFDL+ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 179 DGVIDYREFLRMM 190
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Sgr027650 vs. TAIR 10
Match: AT1G76650.3 (calmodulin-like 38 )

HSP 1 Score: 103.2 bits (256), Expect = 2.3e-22
Identity = 53/133 (39.85%), Postives = 87/133 (65.41%), Query Frame = 0


Query: 59  EAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV 118
           EA F ++DA+ DG+IS EEL+  F ++GE L+ + A   +R  D DGD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 119 EAENEEEE--DLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDG 178
           + ++EEE+  +L+ AF ++ + +G   I P  L+ M  +LG  ++ D+C  MI AFDL+ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 179 DGVIDYREFLRMM 190
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

[Close]

BLAST of Sgr027650 vs. TAIR 10
Match: AT1G76640.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 98.2 bits (243), Expect = 7.5e-21
Identity = 52/135 (38.52%), Postives = 81/135 (60.00%), Query Frame = 0


Query: 59  EAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV 118
           EA F ++DA+ DG+IS EEL+  F ++GE ++ + A   ++  D DGD +L++ +F  L+
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 119 EAENE--EEEDLRRAFEMFE--VEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDL 178
           +  +E  EEE  R+  E F   +  G   I P  L+ M  +LG  ++ D+C  MI AFDL
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMYIADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFDL 143

Query: 179 DGDGVIDYREFLRMM 190
           + DGV+ + EF  MM
Sbjct: 144 NADGVLSFDEFALMM 158

[Close]

BLAST of Sgr027650 vs. TAIR 10
Match: AT1G76650.2 (calmodulin-like 38 )

HSP 1 Score: 94.4 bits (233), Expect = 1.1e-19
Identity = 51/133 (38.35%), Postives = 82/133 (61.65%), Query Frame = 0


Query: 59  EAAFRHLDADGDGKISGEELRSYFASIGESLTRDGARRVIRDFDADGDELLELQDFVRLV 118
           EA F ++DA+ DG+IS EEL+  F ++GE L            D DGD +L+ ++F +L+
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLLS----------DTDGDGMLDFEEFSQLI 104

Query: 119 EAENEEEE--DLRRAFEMFEVEKGCGFIGPAGLQKMFSRLGYVKSNDECMAMIGAFDLDG 178
           + ++EEE+  +L+ AF ++ + +G   I P  L+ M  +LG  ++ D+C  MI AFDL+ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 179 DGVIDYREFLRMM 190
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 166

The following BLAST results are available for this feature:

BLAST of Sgr027650 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139654.1	7.0e-69	76.19	probable calcium-binding protein CML41 [Momordica charantia]	[more]
XP_023535419.1	4.0e-64	72.11	calmodulin-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
KAG7024217.1	8.8e-64	71.05	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. a...	[more]
XP_022974919.1	1.2e-63	71.05	probable calcium-binding protein CML41 [Cucurbita maxima]	[more]
KAG6591338.1	1.5e-63	71.05	putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. s...	[more]

BLAST of Sgr027650 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L3R2	3.9e-30	41.24	Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 ...	[more]
Q8RYK0	1.5e-21	46.10	Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE6	3.3e-21	39.85	Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1	[more]
Q8RYJ9	4.8e-20	45.77	Putative calcium-binding protein CML23 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9SRE7	1.1e-19	38.52	Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1	[more]

BLAST of Sgr027650 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDM6	3.4e-69	76.19	probable calcium-binding protein CML41 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1IIY2	5.6e-64	71.05	probable calcium-binding protein CML41 OS=Cucurbita maxima OX=3661 GN=LOC1114737...	[more]
A0A6J1F7Y7	2.8e-63	70.00	probable calcium-binding protein CML41 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A5A7UHX6	6.8e-62	70.00	Putative calcium-binding protein CML41 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3CT00	6.8e-62	70.00	probable calcium-binding protein CML41 OS=Cucumis melo OX=3656 GN=LOC103503982 P...	[more]

BLAST of Sgr027650 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G50770.1	2.8e-31	41.24	calmodulin-like 41	[more]
AT1G76650.1	2.3e-22	39.85	calmodulin-like 38	[more]
AT1G76650.3	2.3e-22	39.85	calmodulin-like 38	[more]
AT1G76640.1	7.5e-21	38.52	Calcium-binding EF-hand family protein	[more]
AT1G76650.2	1.1e-19	38.35	calmodulin-like 38	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 111..138
None	No IPR available	GENE3D	1.10.238.10		coord: 121..191 e-value: 8.2E-17 score: 63.0
None	No IPR available	GENE3D	1.10.238.10		coord: 42..120 e-value: 3.3E-17 score: 65.0
None	No IPR available	PANTHER	PTHR23050:SF393	CALMODULIN	coord: 16..190
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 16..190
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 57..85 e-value: 1.7E-4 score: 30.9 coord: 164..191 e-value: 0.42 score: 19.7 coord: 93..121 e-value: 1.6 score: 16.2
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 60..118 e-value: 1.5E-12 score: 47.7
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 142..190 e-value: 8.5E-9 score: 35.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 160..191 score: 10.356991
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 53..88 score: 14.122869
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 89..124 score: 9.296966
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 58..118 e-value: 2.70289E-13 score: 59.8689
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 127..190 e-value: 1.76544E-10 score: 52.5501
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 173..185
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 66..78
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 60..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr027650.1	Sgr027650.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding
molecular_function	GO:0030234	enzyme regulator activity