Sgr027459 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr027459 vs. NCBI nr
Match: XP_022137326.1 (transcription initiation factor TFIID subunit 2 [Momordica charantia])

HSP 1 Score: 2349.7 bits (6088), Expect = 0.0e+00
Identity = 1211/1360 (89.04%), Postives = 1244/1360 (91.47%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGA+V HQKLC+SIDID RRIYGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAIVHHQKLCISIDIDKRRIYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPRHQH ESE+SFKAVSSPSSAAD+AGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRHQHAESERSFKAVSSPSSAADSAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSEQQ+QPFLENG+Q AGEDKQNIRLVRIDYWVEKSEVGIHFS+HMAHTD
Sbjct: 121  LINCCKAFKNGSEQQDQPFLENGLQPAGEDKQNIRLVRIDYWVEKSEVGIHFSDHMAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSKDNPPRKTYVYQVD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKDNPPRKTYVYQVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNGLISHMCLPVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGLISHMCLPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQ+F+EPEMA+SSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQVFVEPEMALSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSGLTTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGLTTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
             CKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS
Sbjct: 421  TCKDLYGTQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            ATSV+NRDSDTGWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATSVDNRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALD+RSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDIRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFSVVNALNNFLTDPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAITTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF
Sbjct: 901  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR--------------------------------------WVP 1041
            NNVYLRHYLFCILQVL GR                                        P
Sbjct: 961  NNVYLRHYLFCILQVLGGRPPTLYGVPREYKTLHMGETGTCSEQKKVLTSLIPEFSPPEP 1020

Query: 1042 ISLNALYFEVVFSSTCVGFVYDHHLAHADSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
             S++A+      ++T      D     +DS AIPEVS  GGAIPE PK AMAIVE PREA
Sbjct: 1021 SSVSAVAPMPSIAATLSSEPLDAPKQRSDSLAIPEVSKGGGAIPEVPKQAMAIVEPPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNS+ERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSYERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAE TSISNQNLEEVNSCHDQGS MTASIGSAKLAS GDELGKDFQCTADSSRAFG+F P
Sbjct: 1141 IAETTSISNQNLEEVNSCHDQGSRMTASIGSAKLASYGDELGKDFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASL SRHGKKEKKKDREKKRKRES
Sbjct: 1201 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLHSRHGKKEKKKDREKKRKRES 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKPLP+A+P IKEPPIK T  QLETN
Sbjct: 1261 HKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPLPIAIPPIKEPPIKPTLVQLETN 1320

BLAST of Sgr027459 vs. NCBI nr
Match: XP_022923890.1 (transcription initiation factor TFIID subunit 2 [Cucurbita moschata])

HSP 1 Score: 2303.1 bits (5967), Expect = 0.0e+00
Identity = 1189/1360 (87.43%), Postives = 1229/1360 (90.37%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGAVVRHQKLCLSIDID RR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAVVRHQKLCLSIDIDKRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVESEKSFKAVSSPSSAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRTQHVESEKSFKAVSSPSSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSE+Q+QPFLENGVQ A EDKQNIRLVRIDYWVEKS+VGIHF++H+AHTD
Sbjct: 121  LINCCKAFKNGSEKQDQPFLENGVQPAVEDKQNIRLVRIDYWVEKSDVGIHFNDHIAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSK+NPP KTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKENPPCKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNG ISHMC PVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGFISHMCSPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSH LFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHFLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSG+TTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGITTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTS+SLS
Sbjct: 421  ACKDLYGIQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSQSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT +ENRDSDTGWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATILENRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALDVRSSVESPLLWLRADPEMEYLAEI FHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDVRSSVESPLLWLRADPEMEYLAEIQFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFSVVNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAIATLEMLPQPSFSVVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFRNFNS WQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRNFNSTWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSND VN+DTLVALLLLLEGHMAF
Sbjct: 901  AVLLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDSVNNDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDSGTCSEQKRVLTSLIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAHA-----DSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      DS AIPEVS EG A+ E PK A AI EAPREA
Sbjct: 1021 SSVSAVAPVPCIPVTLSSEPLHTPKPRPDSLAIPEVSKEGAAVVEVPKQATAIAEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNSHERKLPVVKIKVRSSAATSRA+ADNQT ERSHAAP ETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSHERKLPVVKIKVRSSAATSRADADNQTTERSHAAPRETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAEATSISN  LEEVNSCHD GSHMTASIGSAK AS GD+LGK+FQCTADSSRAFG+F P
Sbjct: 1141 IAEATSISNHILEEVNSCHDHGSHMTASIGSAKPASYGDDLGKEFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGLASL SRHGKKEKKKD+EKKRKR+S
Sbjct: 1201 EDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLASLHSRHGKKEKKKDKEKKRKRDS 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKPLP+AMPR+KEPP KSTP QLE+N
Sbjct: 1261 HKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPLPIAMPRMKEPPTKSTPVQLESN 1320

BLAST of Sgr027459 vs. NCBI nr
Match: KAG6584373.1 (Transcription initiation factor TFIID subunit 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 2301.9 bits (5964), Expect = 0.0e+00
Identity = 1188/1360 (87.35%), Postives = 1228/1360 (90.29%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGAVVRHQKLCLSIDID RR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAVVRHQKLCLSIDIDKRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVESEKSFKAVSSPSSAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRTQHVESEKSFKAVSSPSSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSE+Q+QPFLENGVQ A EDKQNIRLVRIDYWVEKS+VGIHF++H+AHTD
Sbjct: 121  LINCCKAFKNGSEKQDQPFLENGVQPAVEDKQNIRLVRIDYWVEKSDVGIHFNDHIAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSK+NPP KTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKENPPCKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNG ISHMC PVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGFISHMCSPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSH LFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHFLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSG+TTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGITTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTS+SLS
Sbjct: 421  ACKDLYGTQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSQSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT +ENRDSDTGWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATILENRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALDVRSSVESPLLWLRADPEMEYLAEI FHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDVRSSVESPLLWLRADPEMEYLAEIQFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQA  TLEMLPQPSFSVVNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQATATLEMLPQPSFSVVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFRNFNS WQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRNFNSTWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSND VN+DTLVALLLLLEGHMAF
Sbjct: 901  AVLLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDSVNNDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDSGTCSEQKRVLTSLIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAHA-----DSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      DS AIPEVS EG A+ E PK A AI EAPREA
Sbjct: 1021 SSVSAVAPVPCIPVTLSSEPLHTPKPRPDSLAIPEVSKEGAAVVEVPKQATAIAEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNSHERKLPVVKIKVRSSAATSRA+ADNQT ERSHAAP ETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSHERKLPVVKIKVRSSAATSRADADNQTTERSHAAPRETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAEATSISN  LEEVNSCHD GSHMTASIGSAK AS GD+LGK+FQCTADSSRAFG+F P
Sbjct: 1141 IAEATSISNHILEEVNSCHDHGSHMTASIGSAKPASYGDDLGKEFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGLASL SRHGKKEKKKD+EKKRKR+S
Sbjct: 1201 EDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLASLHSRHGKKEKKKDKEKKRKRDS 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKPLP+AMPR+KEPP KSTP QLE+N
Sbjct: 1261 HKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPLPIAMPRMKEPPTKSTPVQLESN 1320

BLAST of Sgr027459 vs. NCBI nr
Match: XP_023519716.1 (transcription initiation factor TFIID subunit 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 2300.8 bits (5961), Expect = 0.0e+00
Identity = 1188/1360 (87.35%), Postives = 1228/1360 (90.29%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGAVVRHQKLCLSIDID RR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAVVRHQKLCLSIDIDKRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVESEKSFKAVSSP+SAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRTQHVESEKSFKAVSSPTSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSE+Q+QPFLENGVQ A EDKQNIRLVRIDYWVEKS+VGIHF++H+AHTD
Sbjct: 121  LINCCKAFKNGSEKQDQPFLENGVQPAVEDKQNIRLVRIDYWVEKSDVGIHFNDHIAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSK+NPP KTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKENPPCKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNG ISHMC PVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGFISHMCSPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSH LFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHFLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSG+TTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGITTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTS+SLS
Sbjct: 421  ACKDLYGTQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSQSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT +ENRDSDTGWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATILENRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALDVRSSVESPLLWLRADPEMEYLAEI FHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDVRSSVESPLLWLRADPEMEYLAEIQFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFSVVNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAIATLEMLPQPSFSVVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSIL LASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILVLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFRNFNS WQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRNFNSTWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSND VN+DTLVALLLLLEGHMAF
Sbjct: 901  AVLLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDSVNNDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDSGTCSEQKRVLTSLIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAHA-----DSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      DS AIPEVS EG A+ E PK A AI EAPREA
Sbjct: 1021 SSVSAVAPVPCIPATLSSEPLHTPKPRPDSLAIPEVSKEGAAVVEVPKQATAIAEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNSHERKLPVVKIKVRSSAATSRA+ADNQT ERSHAAP ETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSHERKLPVVKIKVRSSAATSRADADNQTTERSHAAPRETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAEATSISN  LEEVNSCHD GSHMTASIGSAK AS GD+LGK+FQCTADSSRAFG+F P
Sbjct: 1141 IAEATSISNHILEEVNSCHDHGSHMTASIGSAKPASYGDDLGKEFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGLASL SRHGKKEKKKD+EKKRKR+S
Sbjct: 1201 EDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLASLHSRHGKKEKKKDKEKKRKRDS 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKPLP+AMPRIKEPP KSTP QLE+N
Sbjct: 1261 HKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPLPIAMPRIKEPPTKSTPVQLESN 1320

BLAST of Sgr027459 vs. NCBI nr
Match: XP_023000821.1 (transcription initiation factor TFIID subunit 2 [Cucurbita maxima])

HSP 1 Score: 2295.4 bits (5947), Expect = 0.0e+00
Identity = 1184/1360 (87.06%), Postives = 1227/1360 (90.22%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D+KPPDNSGAVVRHQKLCLSIDID RR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDSKPPDNSGAVVRHQKLCLSIDIDKRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVESEKS+KAVSSPSSAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRTQHVESEKSYKAVSSPSSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSE+Q+QPFLENGVQ A EDKQNIRLVRIDYWVEKS+VGIHF++H+AHTD
Sbjct: 121  LINCCKAFKNGSEKQDQPFLENGVQPAVEDKQNIRLVRIDYWVEKSDVGIHFNDHIAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSKDNPP KTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKDNPPCKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNG ISHMC PVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGFISHMCSPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSH LFDEKIIDQTIDTRIKL+YALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHFLFDEKIIDQTIDTRIKLSYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSG+TTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGITTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTS+SLS
Sbjct: 421  ACKDLYGTQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSQSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT +ENRDSDTGWPGMMSIRIYELDGVFDHPVLPM GE WQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATILENRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGEPWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALDVRSSVESPLLWLRADPEMEYLAEI FHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDVRSSVESPLLWLRADPEMEYLAEIQFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQA+ TLEMLPQPSFSVVNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAVATLEMLPQPSFSVVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFRNFNS WQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRNFNSTWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSND VN+DTLVALLLLLEGHMAF
Sbjct: 901  AVLLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDSVNNDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDSGTCSEQKRVLTSLIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAH-----ADSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      DS AIPEVS EG A+ E PK A AI EAPREA
Sbjct: 1021 SSVSAVAPVPCIPATLSSEPLHIPKPKPDSLAIPEVSKEGAAVVEVPKQATAIAEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNSHERKLPVVKIKVRSSAATSRA+ADN T ERSHAAP ETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSHERKLPVVKIKVRSSAATSRADADNLTTERSHAAPRETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAEATSISN  LEEVNSCHD GSHMTASIGSAK AS GD+LGK+FQCTADSSRAFG+F P
Sbjct: 1141 IAEATSISNHILEEVNSCHDHGSHMTASIGSAKPASYGDDLGKEFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGL SL SRHGKKEKKKD+EKKRKR+S
Sbjct: 1201 EDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLFSLHSRHGKKEKKKDKEKKRKRDS 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIER+RLKKEKKQKEKEMAKLLNE+VKPLP+A+PRIKEPP KSTP QLETN
Sbjct: 1261 HKEHRNDPEYIERRRLKKEKKQKEKEMAKLLNEEVKPLPIAIPRIKEPPTKSTPVQLETN 1320

BLAST of Sgr027459 vs. ExPASy Swiss-Prot
Match: Q8LPF0 (Transcription initiation factor TFIID subunit 2 OS=Arabidopsis thaliana OX=3702 GN=TAF2 PE=2 SV=1)

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 809/1420 (56.97%), Postives = 984/1420 (69.30%), Query Frame = 0

Query: 22   MAKPRKPKNTE--DTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLH 81
            MAK RKPKN E    K  +N+GA V HQKL LSID   R+IYG+TELE++VPDIGI GLH
Sbjct: 1    MAKARKPKNEEAPGAKTSENTGAKVLHQKLFLSIDFKKRQIYGYTELEVSVPDIGIVGLH 60

Query: 82   AENLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVP 141
            AENLGI SV VDG+PT FEYYP HQ+ E+E ++ +VS P+SAADAA   Y+  +++E   
Sbjct: 61   AENLGIESVLVDGEPTVFEYYPHHQNSETESNWNSVSDPASAADAAAMEYVGVLKREDTA 120

Query: 142  NLLINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAH 201
            NLLINCCK  K  SEQ +   LENG QS+GE KQN++L+RI+YWVEK E GIHF  ++ H
Sbjct: 121  NLLINCCKPSKDLSEQLDSVTLENGSQSSGEAKQNVKLIRINYWVEKIESGIHFDGNIVH 180

Query: 202  TDNQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQ 261
            TDNQ+RRARCWFPC+DD   RC +DLEFTV  N VAVS G LLYQV+ K++  +KTYVY+
Sbjct: 181  TDNQMRRARCWFPCIDDEYHRCSFDLEFTVPHNFVAVSVGKLLYQVMCKEDTTQKTYVYE 240

Query: 262  VDIPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSV 321
            + IP+  +W+SL  GP EIL D  N LIS++CLP +  +L+NT+EFFH A+S Y+DYLS 
Sbjct: 241  LAIPIAPRWVSLVAGPLEILPDQTNFLISNLCLPHDLSRLRNTMEFFHEAYSYYEDYLSA 300

Query: 322  DFPFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWF 381
            +FPFG YKQ+F+ PEM V+S+  G S+ IFSSH+L+DE++IDQTIDTRIKLA ALA+QWF
Sbjct: 301  NFPFGFYKQVFLPPEMVVTSSTSGASLSIFSSHILYDERVIDQTIDTRIKLASALAKQWF 360

Query: 382  GIYITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSS 441
            G+YITPE+PND+WLLDGLAGFLTD+ IK+ LGNNEARY+RYKANCAVCKADDSG   LSS
Sbjct: 361  GVYITPESPNDDWLLDGLAGFLTDMFIKQFLGNNEARYRRYKANCAVCKADDSGAMCLSS 420

Query: 442  SSACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRS 501
            S +C+D  G +++            A+LQMLEKQMG +SFRKILQ I+SRAKD +++ RS
Sbjct: 421  SPSCRDLFGTHSIGMHGKIRSWKSGAVLQMLEKQMGSDSFRKILQKIISRAKDPSNSIRS 480

Query: 502  LSTKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECT- 561
            LSTKE       IGNLERPFLKEF  RWV S GCP+LR+G SYNKRKN VEMA  RECT 
Sbjct: 481  LSTKEFRQFANKIGNLERPFLKEFFQRWVASYGCPVLRIGLSYNKRKNNVEMAALRECTA 540

Query: 562  -------VTPATS-VENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHS 621
                   V  ATS  E+RD D GWPG+MSIR+YELDG+ DHP LPM G+ WQLLE+ CHS
Sbjct: 541  ALDARLSVIGATSDSESRDVDAGWPGIMSIRVYELDGMSDHPKLPMAGDRWQLLELPCHS 600

Query: 622  KLAARRLQKTKKGSKPDGSDDNTDTPA-LDVRSSVESPLLWLRADPEMEYLAEIHFHQPV 681
            KLAA+R QK KKG KPDG++DN D  A L+ ++S+ESPL W++ADPEMEY+AEIH HQP+
Sbjct: 601  KLAAKRYQKPKKGGKPDGAEDNVDAIAPLENKTSIESPLAWIKADPEMEYIAEIHLHQPL 660

Query: 682  QMWINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAK 741
            QMW+NQLEKD DV+AQAQAI +LE L Q SFS+VNAL N LTD K FWR+RI AA A+AK
Sbjct: 661  QMWVNQLEKDGDVVAQAQAIASLEALKQHSFSIVNALKNVLTDSKVFWRIRIAAAFALAK 720

Query: 742  TASEDTDWAGLLNLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQK 801
            TASE++DWAGL +LIKF+KS+RFDA+ GLPKPNDFRDFPEYFVLEAIPHA+A+VR  + K
Sbjct: 721  TASEESDWAGLQHLIKFYKSRRFDAEIGLPKPNDFRDFPEYFVLEAIPHAIAIVRGAEGK 780

Query: 802  SPREAVEFVLQLLKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLL 861
            SPREAVEF+LQLLKYNDN+GN YSDVFWLA LVQSVG+LEF QQS+ FLA LLKRIDRLL
Sbjct: 781  SPREAVEFILQLLKYNDNSGNSYSDVFWLAVLVQSVGDLEFCQQSLTFLAPLLKRIDRLL 840

Query: 862  QFDRLMPSFNGILTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRA 921
            QFDRLMPS+NGILTISCIRTL Q ALKLS  +S D + +LI PFRN +++ Q+RIE +RA
Sbjct: 841  QFDRLMPSYNGILTISCIRTLAQTALKLSDSISFDHICKLIEPFRNSDTILQIRIEGSRA 900

Query: 922  LLDLEYHCKGIDAALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLV 981
            LLD+EY  KGI +ALLLF+KYL EE SLRGQVKL VH MRLCQI    DS+D V++ TL+
Sbjct: 901  LLDIEYQSKGISSALLLFMKYLVEESSLRGQVKLCVHTMRLCQIAVGCDSDDCVDTVTLL 960

Query: 982  ALLLLLEGHMAFNNVYLRHYLFCILQVLAGR----------------------------- 1041
             LL L + H+ FNN  LR+YLFCI Q+LAGR                             
Sbjct: 961  DLLHLFKSHVVFNNELLRYYLFCIFQILAGRPPTLFGVPKEKPLQLVDVEACIEPKNVFL 1020

Query: 1042 ---------------------------WVPISLNALYF----EVVFSSTCVGFVYDHHL- 1101
                                        VPI    ++     E++       +    HL 
Sbjct: 1021 VPGAEAGEPSLSALGDAKGQSLDVAPYGVPIIPQEMFMPIVPELMLPEPVAAYDETQHLE 1080

Query: 1102 AHADSFAIPEVSNEGGAIPEFPKLAMAIVE--APREA-------------ASVSNSHERK 1161
               +S   P  S+E   + E P       E  A REA              SVS SHE K
Sbjct: 1081 PRMESQNQP--SHENPIVHEIPSDVEGPTEELAHREANPPTKEPQKEPDVVSVSVSHEVK 1140

Query: 1162 LPVVKIKVRSSAATSRAEADNQTIERSH--AAPHETDVGPSSSVSVDAPQRNIAEATSIS 1221
              V++IKVR S ATSRAE   +TIERS      H+ D G +SS SVDAPQR   +A SIS
Sbjct: 1141 KSVIRIKVRPSGATSRAEGSARTIERSQGIVVRHDIDRGQTSSASVDAPQRISTDAVSIS 1200

Query: 1222 NQN-LEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTAD------SSRAFGNFHPE 1281
            NQN +EEVNSCHD GS MTASIGS K AS+GD  GK+ QCTA+      S +A  N    
Sbjct: 1201 NQNHVEEVNSCHDVGSRMTASIGSVKFASEGDIFGKELQCTAESGKPSTSQKADNNNRTV 1260

Query: 1282 DPSSSSIIQDNNVDADA-QKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1326
             PS   +  D++++ +A QKYASLQTLS+ +             +KEKKKD+EKK K   
Sbjct: 1261 PPSFLPL--DHSMENEAQQKYASLQTLSIGK-------------EKEKKKDKEKKEK--- 1320

BLAST of Sgr027459 vs. ExPASy Swiss-Prot
Match: Q32PW3 (Transcription initiation factor TFIID subunit 2 OS=Danio rerio OX=7955 GN=taf2 PE=2 SV=2)

HSP 1 Score: 331.6 bits (849), Expect = 4.0e-89
Identity = 266/937 (28.39%), Postives = 446/937 (47.60%), Query Frame = 0

Query: 26  RKPKNTEDTKPPDNSGAVVRHQKLCL-SIDIDNRRIYGFTELEI--AVPDIGIAGLHAEN 85
           +K K  E  +P       + HQ +C+ +++   + + G+ EL I   V ++    L+++ 
Sbjct: 4   KKDKGFESPRP-----YKLTHQVVCINNVNFQRKSVIGYVELTIFPTVVNLNRIKLNSKQ 63

Query: 86  LGIVSVSVDGDPTEFEYY-PRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 145
             I  V V+     F Y  P  +    E   + ++  SSA  AA    +S+++ +     
Sbjct: 64  CRIYRVRVNDLEAPFIYNDPTLEVCHHESKQRNLNYFSSAYTAA----VSAVDPDAGNGE 123

Query: 146 LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHF-------- 205
           L     + K  SE  +Q            D+  +  V I++ +++ + G+HF        
Sbjct: 124 L-----SIKVPSELWKQ-----------GDEMKVMKVYIEFSLDQPKGGLHFVVPDVEGN 183

Query: 206 ----SNHMAHTDNQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKD 265
               + H+    NQ   +R WFPC+D   + C + LEFTV  ++VAVS G L+  V + D
Sbjct: 184 MAERAAHVFSFGNQ-NSSRFWFPCVDSYSELCTWKLEFTVDASMVAVSCGDLVETVYTHD 243

Query: 266 NPPRKTYVYQVDIPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSA 325
              +KTY Y + IP  A  IS+AVGPFEIL D     ++H CLP     LK+T+ + H  
Sbjct: 244 -MRKKTYHYMLPIPTAAPNISMAVGPFEILVDPYMHEVTHFCLPQLLPLLKHTMSYLHEI 303

Query: 326 FSCYKDYLSVDFPFGSYKQIFI-EPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRI 385
           F  Y++ L+  +P+  +K +F+ E  + VSS     SM IFS++LL    IIDQT  TR 
Sbjct: 304 FEFYEEILTCRYPYSCFKTVFVDEAYVQVSSY---ASMSIFSTNLLHSGLIIDQTPMTRS 363

Query: 386 KLAYALARQWFGIYITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARY--QRYKANCAV 445
            LA ALA+Q+FG +I+  +  DEW+L G++G++  L +KK  G NE R+  +        
Sbjct: 364 FLAQALAQQFFGCFISRMSWADEWVLKGISGYIYGLYLKKTFGVNEYRHWIKEELDKIVE 423

Query: 446 CKADDSGLTTLSSSSACKDCMGPNALV-----------------------AILQMLEKQM 505
            +    G+    + S  K+   P   +                        +++++E ++
Sbjct: 424 YELKIGGVLLHPTFSGGKEKDNPTPHLHFSIKHPHTLSWEYYKMFQCKAHLVMRLIENRI 483

Query: 506 GPESFRKILQNIVSRAKDTTS------------TSRSLSTKEIGNLERPFLKEFIPRWVE 565
             E   ++   ++S A   +S             S S   K I N+    +   I +WV+
Sbjct: 484 SMEFMLQVFNKLLSLASTASSQKYQSHMWSQMLVSTSGFLKSISNVSGKDIGPLIKQWVD 543

Query: 566 SCGCPLLRMGFSYNKRKNMVEMAVSRECTVTPATSVENRDSDTGWPGMMSIRIYELDGVF 625
             G       F++N+++N++E+ + ++ T +             + G + + + ELDG F
Sbjct: 544 QSGVVKFFGSFAFNRKRNVLELEIRQDYTSSGTQK---------YVGPIKVTVQELDGSF 603

Query: 626 DHPVLPMNGESWQLLEIQCHSKLAARRLQKTKKGSKPDGSDDNTDTPALDVRSSVESPLL 685
           +H +     E+    +I CHSK    R  K KK    +G + + D  A+D     +SPLL
Sbjct: 604 NHTL--QIEENSLKHDIPCHSK---SRRNKKKKIPLMNGEEVDMDLSAMD----ADSPLL 663

Query: 686 WLRADPEMEYLAEIHFHQPVQMWINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNF 745
           W+R DP+M  L ++ F Q   MW  QL  ++DV+AQ +AI+ LE  P P      AL + 
Sbjct: 664 WIRIDPDMSILRKVEFEQADFMWQYQLRYERDVVAQEEAILALEKFPTPPSR--RALTDI 723

Query: 746 LTDPKAFWRVRIDAALAMAKTA-SEDTDWAGLLNLIKFFKSQRFDADT--GLPKPNDFRD 805
           L   + F++VR+ A   +AK A S  + W G   +   F ++ F   +   + K N+F  
Sbjct: 724 LEQDQCFYKVRMHACFCLAKIANSMVSTWTGPPAMKSLF-TRMFCCKSCPNIVKTNNFIS 783

Query: 806 FPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQLLKYNDNNGNPYSDVFWLAALVQSV- 865
           F  YF+ + IP A+A +R      PRE + F+L L+KYNDN  N +SD ++ A L+ ++ 
Sbjct: 784 FQSYFLQKTIPVAMAQLRDVQNLCPREVLSFILDLIKYNDNRKNKFSDNYYRAELIDALT 843

Query: 866 ----------GELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGILTISCIRTLTQIAL 895
                      E+         +  +L+ I R L  ++L+PS+   +T+SC+R +    L
Sbjct: 844 NSLTPAISINNEVRTVDSLNADVRLILEEITRFLNMEKLLPSYRNTITVSCLRAIRM--L 885

BLAST of Sgr027459 vs. ExPASy Swiss-Prot
Match: Q6P1X5 (Transcription initiation factor TFIID subunit 2 OS=Homo sapiens OX=9606 GN=TAF2 PE=1 SV=3)

HSP 1 Score: 328.9 bits (842), Expect = 2.6e-88
Identity = 262/915 (28.63%), Postives = 438/915 (47.87%), Query Frame = 0

Query: 46  HQKLCL-SIDIDNRRIYGFTELEI--AVPDIGIAGLHAENLGIVSVSVDGDPTEFEYY-P 105
           HQ +C+ +I+   + + GF EL I   V ++    L+++   I  V ++     F Y  P
Sbjct: 30  HQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQCRIYRVRINDLEAAFIYNDP 89

Query: 106 RHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNLLINCCKAFKSGSEQQEQPFL 165
             +   SE   + ++  S+A  AA    +S+++ +     L       K  SE  +    
Sbjct: 90  TLEVCHSESKQRNLNYFSNAYAAA----VSAVDPDAGNGEL-----CIKVPSELWKH--- 149

Query: 166 ENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHF-----SNHMAHTDNQI------RRARCW 225
                    D+  +  + I++ +++ + G+HF        MA     +         R W
Sbjct: 150 --------VDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFW 209

Query: 226 FPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVDIPVNAQWIS 285
           FPC+D   + C + LEFTV   +VAVSNG L+  V + D   +KT+ Y + IP  A  IS
Sbjct: 210 FPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHD-MRKKTFHYMLTIPTAASNIS 269

Query: 286 LAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDFPFGSYKQIF 345
           LA+GPFEIL D     ++H CLP     LK+T  + H  F  Y++ L+  +P+  +K +F
Sbjct: 270 LAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYPYSCFKTVF 329

Query: 346 IEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGIYITPETPND 405
           I+   A        SM IFS++LL    IID+T  TR  LA +LA+Q+FG +I+  + +D
Sbjct: 330 IDE--AYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQSLAQQFFGCFISRMSWSD 389

Query: 406 EWLLDGLAGFLTDLVIKKNLGNNEARY----QRYKANCAVCKADDSGLTTLSSSSACKD- 465
           EW+L G++G++  L +KK  G NE R+    +  K      K     L  +      KD 
Sbjct: 390 EWVLKGISGYIYGLWMKKTFGVNEYRHWIKEELDKIVAYELKTGGVLLHPIFGGGKEKDN 449

Query: 466 --------CMGPNALV------------AILQMLEKQMGPESFRKILQNIVSRAKDTTS- 525
                      P+ L              +++++E ++  E   ++   ++S A   +S 
Sbjct: 450 PASHLHFSIKHPHTLSWEYYSMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQ 509

Query: 526 -----------TSRSLSTKEIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEM 585
                       S S   K I N+    ++  I +WV+  G       F++N+++N++E+
Sbjct: 510 KFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLEL 569

Query: 586 AVSRECTVTPATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSK 645
            + ++ T +P T          + G + + + ELDG F+H +     E+    +I CHSK
Sbjct: 570 EIKQDYT-SPGTQ--------KYVGPLKVTVQELDGSFNHTL--QIEENSLKHDIPCHSK 629

Query: 646 LAARRLQKTKKGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQM 705
               R  K KK    +G + + D  A+D     +SPLLW+R DP+M  L ++ F Q   M
Sbjct: 630 ---SRRNKKKKIPLMNGEEVDMDLSAMD----ADSPLLWIRIDPDMSVLRKVEFEQADFM 689

Query: 706 WINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTA 765
           W  QL  ++DV+AQ ++I+ LE  P P+  +  AL + L   + F+RVR+ A   +AK A
Sbjct: 690 WQYQLRYERDVVAQQESILALEKFPTPASRL--ALTDILEQEQCFYRVRMSACFCLAKIA 749

Query: 766 -SEDTDWAGLLNLIKFFKSQRFDADT--GLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQ 825
            S  + W G   +   F ++ F   +   + K N+F  F  YF+ + +P A+A++R    
Sbjct: 750 NSMVSTWTGPPAMKSLF-TRMFCCKSCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHN 809

Query: 826 KSPREAVEFVLQLLKYNDNNGNPYSDVFWLA----ALVQSVGELEFGQQSILFLASL--- 885
             P+E + F+L L+KYNDN  N +SD ++ A    AL  SV         +  L +L   
Sbjct: 810 LCPKEVLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPD 869

Query: 886 ----LKRIDRLLQFDRLMPSFNGILTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFN 895
               L+ I R L  ++L+PS+   +T+SC+R +    L+ +G +  D    L + +  + 
Sbjct: 870 VRLILEEITRFLNMEKLLPSYRHTITVSCLRAIR--VLQKNGHVPSDPA--LFKSYAEYG 896

BLAST of Sgr027459 vs. ExPASy Swiss-Prot
Match: Q5ZIT8 (Transcription initiation factor TFIID subunit 2 OS=Gallus gallus OX=9031 GN=TAF2 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 2.2e-87
Identity = 264/915 (28.85%), Postives = 441/915 (48.20%), Query Frame = 0

Query: 46  HQKLCL-SIDIDNRRIYGFTELEI--AVPDIGIAGLHAENLGIVSVSVDGDPTEFEYY-P 105
           HQ +C+ +I+   + + G+ EL I   V ++    L+++   I  V V+     F Y  P
Sbjct: 20  HQVVCINNINFQRKSVVGYVELTIFPTVANLNRIKLNSKQCRIYRVRVNDLEAAFIYNDP 79

Query: 106 RHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNLLINCCKAFKSGSEQQEQPFL 165
             +    E   + ++  S+A  AA    +S+++ +     L       K  SE  +    
Sbjct: 80  TLEVCHHESKQRNLNYFSNAYAAA----VSAVDPDAGNGEL-----CIKVPSELWKH--- 139

Query: 166 ENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHF-----SNHMAHTDNQI------RRARCW 225
                    D+  +  V I++ +++ + G+HF        MA     +         R W
Sbjct: 140 --------VDELKVLKVHINFSLDQPKGGLHFVVPNMEGSMAERGAHVFSCGYQNSTRFW 199

Query: 226 FPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVDIPVNAQWIS 285
           FPC+D   + C + LE+TV   +VAVSNG L+  V + D   +KT+ Y + IP  A  IS
Sbjct: 200 FPCVDSYSELCTWKLEYTVDAAMVAVSNGDLVETVYTHD-MRKKTFHYMLAIPTAASNIS 259

Query: 286 LAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDFPFGSYKQIF 345
           LA+GPFEIL D     ++H CLP     LK+T  + H  F  Y++ L+  +P+  +K +F
Sbjct: 260 LAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYLHEVFEFYEEILTCRYPYSCFKTVF 319

Query: 346 IEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGIYITPETPND 405
           I+   A        SM IFS++LL    IID+T  TR  LA ALA+Q+FG +I+  + +D
Sbjct: 320 IDE--AYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQALAQQFFGCFISRMSWSD 379

Query: 406 EWLLDGLAGFLTDLVIKKNLGNNEARYQ-RYKANCAVCKADDSG---LTTLSSSSACKD- 465
           EW+L G++G++  L +KK  G NE R+  + + +  V     +G   L  +      KD 
Sbjct: 380 EWVLKGISGYIYGLWMKKTFGVNEYRHWIKQELDQIVAYELKTGGVLLHPIFGGGKEKDN 439

Query: 466 --------CMGPNALV------------AILQMLEKQMGPESFRKILQNIVSRAKDTTS- 525
                      P+ L              +++++E ++  E   ++   ++S A   +S 
Sbjct: 440 PASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQ 499

Query: 526 -----------TSRSLSTKEIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEM 585
                       S S   K I N+    ++  I +WV+  G       F++N+++N++E+
Sbjct: 500 KFQSHMWSQMLVSTSGFLKSISNVSGKDIQPLIKQWVDQSGVVKFYGSFAFNRKRNVLEL 559

Query: 586 AVSRECTVTPATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSK 645
            + ++ T +P T          + G + + + ELDG F+H +     E+    +I CHSK
Sbjct: 560 EIKQDYT-SPGTQ--------KYVGPLKVTVQELDGSFNHTL--QIEENSLKHDIPCHSK 619

Query: 646 LAARRLQKTKKGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQM 705
               R  K KK    +G + + D  A+D     +SPLLW+R DP+M  L ++ F Q   M
Sbjct: 620 ---SRRNKKKKIPLMNGEEVDMDLSAMD----ADSPLLWIRIDPDMSVLRKVEFEQSDFM 679

Query: 706 WINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTA 765
           W  QL  ++DV+AQ +AI+ LE  P P+  +  AL + L   + F+RVR+ A   +AK A
Sbjct: 680 WQYQLRYERDVVAQEEAILALEKFPTPASRL--ALTDILEQEQCFYRVRMLACFCLAKIA 739

Query: 766 -SEDTDWAGLLNLIKFFKSQRFDADT--GLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQ 825
            S  + W G   +   F ++ F   T   + K N+F +F  YF+ + +P A+A++R    
Sbjct: 740 NSMVSTWTGPPAMKSLF-TRMFCCKTCPNIVKTNNFMNFQSYFLQKTMPVAMALLRDVHN 799

Query: 826 KSPREAVEFVLQLLKYNDNNGNPYSDVFWLA----ALVQSVGELEFGQQSILFLASL--- 885
             P+E + F+L L+KYNDN  N +SD ++ A    AL  SV         +  L +L   
Sbjct: 800 LCPKEVLMFILDLIKYNDNRKNKFSDNYYRAELIDALANSVTPAVSVNNEVRTLDNLNPD 859

Query: 886 ----LKRIDRLLQFDRLMPSFNGILTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFN 895
               L+ I R L  ++L+PS+   +T+SC++ +    L+ +G +  D    L + +  + 
Sbjct: 860 VRLILEEITRFLNMEKLLPSYRHTITVSCLKAIR--VLQKNGHVPSDPA--LFKSYAEYG 886

BLAST of Sgr027459 vs. ExPASy Swiss-Prot
Match: Q8C176 (Transcription initiation factor TFIID subunit 2 OS=Mus musculus OX=10090 GN=Taf2 PE=2 SV=2)

HSP 1 Score: 325.5 bits (833), Expect = 2.8e-87
Identity = 260/915 (28.42%), Postives = 439/915 (47.98%), Query Frame = 0

Query: 46  HQKLCL-SIDIDNRRIYGFTELEI--AVPDIGIAGLHAENLGIVSVSVDGDPTEFEYY-P 105
           HQ +C+ +I+   + + GF EL I   V ++    L+++   I  V ++     F Y  P
Sbjct: 20  HQVVCINNINFQRKSVVGFVELTIFPTVANLNRIKLNSKQCRIYRVRINDLEAAFIYNDP 79

Query: 106 RHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNLLINCCKAFKSGSEQQEQPFL 165
             +   SE   + ++  S+A  AA    +S+++ +     L       K  SE  +    
Sbjct: 80  TLEVCHSESKQRNLNYFSNAYAAA----VSAVDPDAGNGEL-----CIKVPSELWKH--- 139

Query: 166 ENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHF-----SNHMAHTDNQI------RRARCW 225
                    D+  +  + I++ +++ + G+HF        MA     +         R W
Sbjct: 140 --------VDELKVLKIHINFSLDQPKGGLHFVVPSVEGSMAERGAHVFSCGYQNSTRFW 199

Query: 226 FPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVDIPVNAQWIS 285
           FPC+D   + C + LEFTV   +VAVSNG L+  V + D   +KT+ Y + IP  A  IS
Sbjct: 200 FPCVDSYSELCTWKLEFTVDAAMVAVSNGDLVETVYTHD-MRKKTFHYMLTIPTAASNIS 259

Query: 286 LAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDFPFGSYKQIF 345
           LA+GPFEIL D     ++H CLP     LK+T  + H  F  Y++ L+  +P+  +K +F
Sbjct: 260 LAIGPFEILVDPYMHEVTHFCLPQLLPLLKHTTSYIHEVFEFYEEILTCRYPYSCFKTVF 319

Query: 346 IEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGIYITPETPND 405
           I+   A        SM IFS++LL    IID+T  TR  LA ALA+Q+FG +I+  + +D
Sbjct: 320 IDE--AYVEVAAYASMSIFSTNLLHSAMIIDETPLTRRCLAQALAQQFFGCFISRMSWSD 379

Query: 406 EWLLDGLAGFLTDLVIKKNLGNNEARY----QRYKANCAVCKADDSGLTTLSSSSACKD- 465
           EW+L G++G++  L +KK  G NE  +    +  K      K     L  +      KD 
Sbjct: 380 EWVLKGISGYIYGLWMKKTFGVNEYHHWIKEELDKIVAYELKTGGVLLHPIFGGGKEKDN 439

Query: 466 --------CMGPNALV------------AILQMLEKQMGPESFRKILQNIVSRAKDTTS- 525
                      P+ L              +++++E ++  E   ++   ++S A   +S 
Sbjct: 440 PASHLHFSIKHPHTLSWEYYTMFQCKAHLVMRLIENRISMEFMLQVFNKLLSLASTASSQ 499

Query: 526 -------TSRSLST----KEIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEM 585
                  +   +ST    K I N+    ++  I +W++  G       F++N+++N++E+
Sbjct: 500 KFQSHMWSQMLVSTYGFLKSISNVSGKDIQPLIKQWLDQSGVVKFYGSFAFNRKRNVLEL 559

Query: 586 AVSRECTVTPATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSK 645
            + ++ T +P T          + G + + + ELDG F+H +     E+    +I CHSK
Sbjct: 560 EIKQDYT-SPGTQ--------KYVGPLKVTVQELDGSFNHTL--QIEENSLKHDIPCHSK 619

Query: 646 LAARRLQKTKKGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQM 705
               R  K KK    +G + + D  A++     +SPLLW+R DP+M  L ++ F Q   M
Sbjct: 620 ---SRRNKKKKIPLMNGEEVDMDLSAME----ADSPLLWIRIDPDMSVLRKVEFEQADFM 679

Query: 706 WINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTA 765
           W  +L  ++DV+AQ ++I+ LE  P P+  +  AL + L   + F+RVR+ A   +AK A
Sbjct: 680 WQYELRYERDVVAQQESILALEKFPTPASRL--ALTDILEQEQCFYRVRMSACFCLAKIA 739

Query: 766 -SEDTDWAGLLNLIKFFKSQRFDADT--GLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQ 825
            S  + W G   +   F ++ F   T   + K N+F  F  YF+ + +P A+A++R    
Sbjct: 740 NSMVSTWTGPPAMKSLF-TRMFCCKTCPNIVKTNNFMSFQSYFLQKTMPVAMALLRDVHN 799

Query: 826 KSPREAVEFVLQLLKYNDNNGNPYSDVFWLA----ALVQSVGELEFGQQSILFLASL--- 885
             P+E + F+L L+KYNDN  N +SD ++ A    AL  SV         +  L +L   
Sbjct: 800 LCPKEVLTFILDLIKYNDNRKNKFSDNYYRAEMIDALANSVTPAVSVNNEVRTLDNLNPD 859

Query: 886 ----LKRIDRLLQFDRLMPSFNGILTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFN 895
               L+ I R L  ++L+PS+   +T+SC+R +    L+ +G +  D    L + +  + 
Sbjct: 860 VRLILEEITRFLNMEKLLPSYRHTITVSCLRAIR--VLQKNGHVPSDA--SLFKSYAEYG 886

BLAST of Sgr027459 vs. ExPASy TrEMBL
Match: A0A6J1C691 (Transcription initiation factor TFIID subunit 2 OS=Momordica charantia OX=3673 GN=LOC111008817 PE=3 SV=1)

HSP 1 Score: 2349.7 bits (6088), Expect = 0.0e+00
Identity = 1211/1360 (89.04%), Postives = 1244/1360 (91.47%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGA+V HQKLC+SIDID RRIYGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAIVHHQKLCISIDIDKRRIYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPRHQH ESE+SFKAVSSPSSAAD+AGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRHQHAESERSFKAVSSPSSAADSAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSEQQ+QPFLENG+Q AGEDKQNIRLVRIDYWVEKSEVGIHFS+HMAHTD
Sbjct: 121  LINCCKAFKNGSEQQDQPFLENGLQPAGEDKQNIRLVRIDYWVEKSEVGIHFSDHMAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSKDNPPRKTYVYQVD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKDNPPRKTYVYQVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNGLISHMCLPVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGLISHMCLPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQ+F+EPEMA+SSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQVFVEPEMALSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSGLTTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGLTTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
             CKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS
Sbjct: 421  TCKDLYGTQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            ATSV+NRDSDTGWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATSVDNRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALD+RSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDIRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFSVVNALNNFLTDPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAITTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF
Sbjct: 901  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR--------------------------------------WVP 1041
            NNVYLRHYLFCILQVL GR                                        P
Sbjct: 961  NNVYLRHYLFCILQVLGGRPPTLYGVPREYKTLHMGETGTCSEQKKVLTSLIPEFSPPEP 1020

Query: 1042 ISLNALYFEVVFSSTCVGFVYDHHLAHADSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
             S++A+      ++T      D     +DS AIPEVS  GGAIPE PK AMAIVE PREA
Sbjct: 1021 SSVSAVAPMPSIAATLSSEPLDAPKQRSDSLAIPEVSKGGGAIPEVPKQAMAIVEPPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNS+ERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSYERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAE TSISNQNLEEVNSCHDQGS MTASIGSAKLAS GDELGKDFQCTADSSRAFG+F P
Sbjct: 1141 IAETTSISNQNLEEVNSCHDQGSRMTASIGSAKLASYGDELGKDFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASL SRHGKKEKKKDREKKRKRES
Sbjct: 1201 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLHSRHGKKEKKKDREKKRKRES 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKPLP+A+P IKEPPIK T  QLETN
Sbjct: 1261 HKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPLPIAIPPIKEPPIKPTLVQLETN 1320

BLAST of Sgr027459 vs. ExPASy TrEMBL
Match: A0A6J1ED82 (Transcription initiation factor TFIID subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111431475 PE=3 SV=1)

HSP 1 Score: 2303.1 bits (5967), Expect = 0.0e+00
Identity = 1189/1360 (87.43%), Postives = 1229/1360 (90.37%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGAVVRHQKLCLSIDID RR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAVVRHQKLCLSIDIDKRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVESEKSFKAVSSPSSAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRTQHVESEKSFKAVSSPSSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSE+Q+QPFLENGVQ A EDKQNIRLVRIDYWVEKS+VGIHF++H+AHTD
Sbjct: 121  LINCCKAFKNGSEKQDQPFLENGVQPAVEDKQNIRLVRIDYWVEKSDVGIHFNDHIAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSK+NPP KTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKENPPCKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNG ISHMC PVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGFISHMCSPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSH LFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHFLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSG+TTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGITTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTS+SLS
Sbjct: 421  ACKDLYGIQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSQSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT +ENRDSDTGWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATILENRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALDVRSSVESPLLWLRADPEMEYLAEI FHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDVRSSVESPLLWLRADPEMEYLAEIQFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFSVVNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAIATLEMLPQPSFSVVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFRNFNS WQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRNFNSTWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSND VN+DTLVALLLLLEGHMAF
Sbjct: 901  AVLLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDSVNNDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDSGTCSEQKRVLTSLIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAHA-----DSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      DS AIPEVS EG A+ E PK A AI EAPREA
Sbjct: 1021 SSVSAVAPVPCIPVTLSSEPLHTPKPRPDSLAIPEVSKEGAAVVEVPKQATAIAEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNSHERKLPVVKIKVRSSAATSRA+ADNQT ERSHAAP ETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSHERKLPVVKIKVRSSAATSRADADNQTTERSHAAPRETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAEATSISN  LEEVNSCHD GSHMTASIGSAK AS GD+LGK+FQCTADSSRAFG+F P
Sbjct: 1141 IAEATSISNHILEEVNSCHDHGSHMTASIGSAKPASYGDDLGKEFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGLASL SRHGKKEKKKD+EKKRKR+S
Sbjct: 1201 EDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLASLHSRHGKKEKKKDKEKKRKRDS 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKPLP+AMPR+KEPP KSTP QLE+N
Sbjct: 1261 HKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPLPIAMPRMKEPPTKSTPVQLESN 1320

BLAST of Sgr027459 vs. ExPASy TrEMBL
Match: A0A6J1KGW7 (Transcription initiation factor TFIID subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111495155 PE=3 SV=1)

HSP 1 Score: 2295.4 bits (5947), Expect = 0.0e+00
Identity = 1184/1360 (87.06%), Postives = 1227/1360 (90.22%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D+KPPDNSGAVVRHQKLCLSIDID RR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDSKPPDNSGAVVRHQKLCLSIDIDKRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVESEKS+KAVSSPSSAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRTQHVESEKSYKAVSSPSSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFK+GSE+Q+QPFLENGVQ A EDKQNIRLVRIDYWVEKS+VGIHF++H+AHTD
Sbjct: 121  LINCCKAFKNGSEKQDQPFLENGVQPAVEDKQNIRLVRIDYWVEKSDVGIHFNDHIAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSKDNPP KTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKDNPPCKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA WISLAVGPFEILADHQNG ISHMC PVNSLKLK+TVEFFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNAHWISLAVGPFEILADHQNGFISHMCSPVNSLKLKHTVEFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSH LFDEKIIDQTIDTRIKL+YALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHFLFDEKIIDQTIDTRIKLSYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSG+TTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGITTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTS+SLS
Sbjct: 421  ACKDLYGTQRIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSQSLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP
Sbjct: 481  TKEFRHLANKIGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT +ENRDSDTGWPGMMSIRIYELDGVFDHPVLPM GE WQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATILENRDSDTGWPGMMSIRIYELDGVFDHPVLPMTGEPWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALDVRSSVESPLLWLRADPEMEYLAEI FHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDVRSSVESPLLWLRADPEMEYLAEIQFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQA+ TLEMLPQPSFSVVNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAVATLEMLPQPSFSVVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFRNFNS WQVRIEATRALLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRNFNSTWQVRIEATRALLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSND VN+DTLVALLLLLEGHMAF
Sbjct: 901  AVLLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDSVNNDTLVALLLLLEGHMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDSGTCSEQKRVLTSLIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAH-----ADSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      DS AIPEVS EG A+ E PK A AI EAPREA
Sbjct: 1021 SSVSAVAPVPCIPATLSSEPLHIPKPKPDSLAIPEVSKEGAAVVEVPKQATAIAEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVSNSHERKLPVVKIKVRSSAATSRA+ADN T ERSHAAP ETDVGPSSSVSVDAPQRN
Sbjct: 1081 ASVSNSHERKLPVVKIKVRSSAATSRADADNLTTERSHAAPRETDVGPSSSVSVDAPQRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTADSSRAFGNFHP 1221
            IAEATSISN  LEEVNSCHD GSHMTASIGSAK AS GD+LGK+FQCTADSSRAFG+F P
Sbjct: 1141 IAEATSISNHILEEVNSCHDHGSHMTASIGSAKPASYGDDLGKEFQCTADSSRAFGHFQP 1200

Query: 1222 EDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1281
            EDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGL SL SRHGKKEKKKD+EKKRKR+S
Sbjct: 1201 EDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLFSLHSRHGKKEKKKDKEKKRKRDS 1260

Query: 1282 HKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLETN 1326
            HKEH NDPEYIER+RLKKEKKQKEKEMAKLLNE+VKPLP+A+PRIKEPP KSTP QLETN
Sbjct: 1261 HKEHRNDPEYIERRRLKKEKKQKEKEMAKLLNEEVKPLPIAIPRIKEPPTKSTPVQLETN 1320

BLAST of Sgr027459 vs. ExPASy TrEMBL
Match: A0A1S3C357 (Transcription initiation factor TFIID subunit 2 OS=Cucumis melo OX=3656 GN=LOC103496328 PE=3 SV=1)

HSP 1 Score: 2274.2 bits (5892), Expect = 0.0e+00
Identity = 1174/1361 (86.26%), Postives = 1222/1361 (89.79%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGAVVRHQKLCLSIDIDNRR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAVVRHQKLCLSIDIDNRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGI+SVSVDGDPTEFEYYPR QHVESE+SFKAV+SPSSAADAAGS+Y+SSIEKELVPNL
Sbjct: 61   NLGILSVSVDGDPTEFEYYPRPQHVESERSFKAVASPSSAADAAGSIYMSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFKSGSEQQ+QPFLENGVQ A EDKQN+RLVRIDYWVEKSEVGIHF N +AHTD
Sbjct: 121  LINCCKAFKSGSEQQDQPFLENGVQPADEDKQNVRLVRIDYWVEKSEVGIHFYNRLAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSKDNPPRKTYVY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKDNPPRKTYVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA+WISLAVGPFEILADHQN LISHMC PVNSLKLK+TV+FFH AFSCYKDYLSVDF
Sbjct: 241  IPVNARWISLAVGPFEILADHQNVLISHMCSPVNSLKLKHTVDFFHGAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANCAVC+ADDSGLTTLSSS+
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCAVCRADDSGLTTLSSSA 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVS AKDT S S+ LS
Sbjct: 421  ACKDLHGTQCIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSHAKDTGSASQLLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEF PRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECT TP
Sbjct: 481  TKEFRQLANKIGNLERPFLKEFFPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTATP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            ATSVENRDSD GWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATSVENRDSDAGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALD+RSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDIRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFS+VNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAIATLEMLPQPSFSIVNALNNFLRDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVR TDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRGTDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFR+FNS+WQVRIEATR+LLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRDFNSIWQVRIEATRSLLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEE SLRGQ KL VHVMRLCQIMRRS SND+VN+DTLVALLLLLEG+MAF
Sbjct: 901  ATLLLFIKYLEEENSLRGQAKLAVHVMRLCQIMRRSGSNDVVNNDTLVALLLLLEGNMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNV LRHYLFCILQVLAGR      VP     L+                          
Sbjct: 961  NNVCLRHYLFCILQVLAGRPPTLYGVPREYKTLHMGDTGTCSEQKRMLTSLIPEFNPPEQ 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAHA-----DSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         HA     DS A+PE+S EGG I E PK AMAIVEAPREA
Sbjct: 1021 SSVSAVAPMPCIPASLSSEPLHAPTPRPDSLAVPELSKEGGEIAEVPKQAMAIVEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVS+SHERKLPVVKIKVRSSAATSRA+ADN T ERSHA P ETDVGPSSSVSVDAP RN
Sbjct: 1081 ASVSSSHERKLPVVKIKVRSSAATSRADADNLTTERSHAVPRETDVGPSSSVSVDAPPRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTAD-SSRAFGNFH 1221
            IAEATSISN+ LEEVNSCHD GSHMTASIGSAKLAS GDELGK+FQCTAD SSRAFG+F 
Sbjct: 1141 IAEATSISNRILEEVNSCHDHGSHMTASIGSAKLASYGDELGKEFQCTADSSSRAFGHFQ 1200

Query: 1222 PEDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRE 1281
            PEDPSSSSIIQDNN+DADAQKYASLQTLSLPQ+DHGLAS  SRHGKKEKKKD+EKKRKRE
Sbjct: 1201 PEDPSSSSIIQDNNIDADAQKYASLQTLSLPQNDHGLASSHSRHGKKEKKKDKEKKRKRE 1260

Query: 1282 SHKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLET 1326
            SHKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKP P+AMPRIKEPP KSTP QLET
Sbjct: 1261 SHKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPQPLAMPRIKEPPTKSTPMQLET 1320

BLAST of Sgr027459 vs. ExPASy TrEMBL
Match: A0A0A0LQC8 (Transcription initiation factor TFIID subunit 2 OS=Cucumis sativus OX=3659 GN=Csa_1G042170 PE=3 SV=1)

HSP 1 Score: 2272.3 bits (5887), Expect = 0.0e+00
Identity = 1176/1362 (86.34%), Postives = 1224/1362 (89.87%), Query Frame = 0

Query: 22   MAKPRKPKNTEDTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLHAE 81
            MAKPRKPKNT+D KPPDNSGAVVRHQKLCLSIDIDNRR+YGFTELEIAVPDIGI GLHAE
Sbjct: 1    MAKPRKPKNTDDAKPPDNSGAVVRHQKLCLSIDIDNRRVYGFTELEIAVPDIGIVGLHAE 60

Query: 82   NLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVPNL 141
            NLGIVSVSVDGDPTEFEYYPR QHVE+E+SFKAVSSPSSAADAAGS+YLSSIEKELVPNL
Sbjct: 61   NLGIVSVSVDGDPTEFEYYPRPQHVENERSFKAVSSPSSAADAAGSIYLSSIEKELVPNL 120

Query: 142  LINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAHTD 201
            LINCCKAFKSGSEQQ+QPFLENGVQ+A EDKQN+RLVRIDYWVEKSEVGIHF N MAHTD
Sbjct: 121  LINCCKAFKSGSEQQDQPFLENGVQTADEDKQNVRLVRIDYWVEKSEVGIHFYNRMAHTD 180

Query: 202  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQVD 261
            NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNG+LLYQVLSKDNPPRKT+VY+VD
Sbjct: 181  NQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGILLYQVLSKDNPPRKTFVYRVD 240

Query: 262  IPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSVDF 321
            IPVNA+WISLAVGPFEILADHQN LISHMC PVNSLKLK+TV+FFHSAFSCYKDYLSVDF
Sbjct: 241  IPVNARWISLAVGPFEILADHQNVLISHMCSPVNSLKLKHTVDFFHSAFSCYKDYLSVDF 300

Query: 322  PFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 381
            PFGSYKQIFIEPE+AVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI
Sbjct: 301  PFGSYKQIFIEPEIAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWFGI 360

Query: 382  YITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSSSS 441
            YITPE PNDEWLLDGLAGFLTDL IKKNLGNNEARYQRYKANC+VC+ADD GLTTLSSSS
Sbjct: 361  YITPEAPNDEWLLDGLAGFLTDLFIKKNLGNNEARYQRYKANCSVCRADDCGLTTLSSSS 420

Query: 442  ACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRSLS 501
            ACKD  G   +           VAILQMLEKQMGPESFRKILQNIVS AKDT STS+ LS
Sbjct: 421  ACKDLHGTQCIGIYGKIRSWKSVAILQMLEKQMGPESFRKILQNIVSHAKDTGSTSQLLS 480

Query: 502  TKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTVTP 561
            TKE       IGNLERPFLKEF PRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECT TP
Sbjct: 481  TKEFRQLANKIGNLERPFLKEFFPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECTATP 540

Query: 562  ATSVENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHSKLAARRLQKTK 621
            AT+VENRDSD GWPGMMSIRIYELDGVFDHPVLPM GESWQLLEIQCHSKLAARRLQKTK
Sbjct: 541  ATNVENRDSDAGWPGMMSIRIYELDGVFDHPVLPMTGESWQLLEIQCHSKLAARRLQKTK 600

Query: 622  KGSKPDGSDDNTDTPALDVRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 681
            KGSKPDGSDDN D PALD+RSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD
Sbjct: 601  KGSKPDGSDDNADIPALDIRSSVESPLLWLRADPEMEYLAEIHFHQPVQMWINQLEKDKD 660

Query: 682  VIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAKTASEDTDWAGLL 741
            VIAQAQAI TLEMLPQPSFS+VNALNNFL DPKAFWRVRI+AALAMAKTASEDTDWAGLL
Sbjct: 661  VIAQAQAIATLEMLPQPSFSIVNALNNFLKDPKAFWRVRIEAALAMAKTASEDTDWAGLL 720

Query: 742  NLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQKSPREAVEFVLQL 801
            NLIKFFKSQRFDADTGLPKPN+FRDFPEYFVLEAIPHAVAMVR TDQKSPREAVEFVLQL
Sbjct: 721  NLIKFFKSQRFDADTGLPKPNEFRDFPEYFVLEAIPHAVAMVRGTDQKSPREAVEFVLQL 780

Query: 802  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSFNGI 861
            LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPS+NGI
Sbjct: 781  LKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLLQFDRLMPSYNGI 840

Query: 862  LTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRALLDLEYHCKGID 921
            LTISCIRTLTQIALKLSGLLSLDR+ ELIRPFR+FNSMWQVRIEATR+LLDLEYHC GID
Sbjct: 841  LTISCIRTLTQIALKLSGLLSLDRIIELIRPFRDFNSMWQVRIEATRSLLDLEYHCNGID 900

Query: 922  AALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLVALLLLLEGHMAF 981
            A LLLFIKYLEEE SLRGQVKL VHVMRLCQIMRRS SND+VN+DTLVALLLLLEG+MAF
Sbjct: 901  ATLLLFIKYLEEENSLRGQVKLAVHVMRLCQIMRRSGSNDVVNNDTLVALLLLLEGNMAF 960

Query: 982  NNVYLRHYLFCILQVLAGR-----WVPISLNALYF------------------------- 1041
            NNVYLRHYLF ILQVL+GR      VP     L+                          
Sbjct: 961  NNVYLRHYLFSILQVLSGRSPTLYGVPREYKTLHMGDTGTFSEQKRMLTSIIPEFNPPEP 1020

Query: 1042 ---EVVFSSTCVGFVYDHHLAHA-----DSFAIPEVSNEGGAIPEFPKLAMAIVEAPREA 1101
                 V    C+         H      D+ A+PE+S E GAI E PK AMAIVEAPREA
Sbjct: 1021 SSVSAVAPMPCIPATLSSEPLHVPTPRPDNLAVPELSKEEGAIAEDPKQAMAIVEAPREA 1080

Query: 1102 ASVSNSHERKLPVVKIKVRSSAATSRAEADNQTIERSHAAPHETDVGPSSSVSVDAPQRN 1161
            ASVS+SHERKLPVVKIKVRSSAATSRA+ADN T ERSHAAP ETDVGPSSSVSVDAP RN
Sbjct: 1081 ASVSSSHERKLPVVKIKVRSSAATSRADADNLTTERSHAAPRETDVGPSSSVSVDAPPRN 1140

Query: 1162 IAEATSISNQNLEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTAD-SSRAFGNFH 1221
             AEATSISN+ LEEVNSCHD GSHMTASIGSAKLAS GDELGK+FQCTAD SSRAFG+F 
Sbjct: 1141 TAEATSISNRILEEVNSCHDHGSHMTASIGSAKLASYGDELGKEFQCTADSSSRAFGHFQ 1200

Query: 1222 PEDPSSSSIIQDNNVDADAQKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRE 1281
            PEDPSSSSIIQDNN+DADAQKYASLQTLSLPQHDHGLAS  SRHGKKEKKKD+EKKRKRE
Sbjct: 1201 PEDPSSSSIIQDNNIDADAQKYASLQTLSLPQHDHGLASSHSRHGKKEKKKDKEKKRKRE 1260

Query: 1282 SHKEHHNDPEYIERKRLKKEKKQKEKEMAKLLNEDVKPLPVAMPRIKEPPIKSTPEQLET 1326
            SHKEH NDPEYIERKRLKKEKKQKEKEMAKLLNE+VKP P AMPRIKEPP KSTP QLET
Sbjct: 1261 SHKEHRNDPEYIERKRLKKEKKQKEKEMAKLLNEEVKPQPTAMPRIKEPPTKSTPVQLET 1320

BLAST of Sgr027459 vs. TAIR 10
Match: AT1G73960.1 (TBP-associated factor 2 )

HSP 1 Score: 1459.9 bits (3778), Expect = 0.0e+00
Identity = 809/1420 (56.97%), Postives = 984/1420 (69.30%), Query Frame = 0

Query: 22   MAKPRKPKNTE--DTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLH 81
            MAK RKPKN E    K  +N+GA V HQKL LSID   R+IYG+TELE++VPDIGI GLH
Sbjct: 1    MAKARKPKNEEAPGAKTSENTGAKVLHQKLFLSIDFKKRQIYGYTELEVSVPDIGIVGLH 60

Query: 82   AENLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVP 141
            AENLGI SV VDG+PT FEYYP HQ+ E+E ++ +VS P+SAADAA   Y+  +++E   
Sbjct: 61   AENLGIESVLVDGEPTVFEYYPHHQNSETESNWNSVSDPASAADAAAMEYVGVLKREDTA 120

Query: 142  NLLINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAH 201
            NLLINCCK  K  SEQ +   LENG QS+GE KQN++L+RI+YWVEK E GIHF  ++ H
Sbjct: 121  NLLINCCKPSKDLSEQLDSVTLENGSQSSGEAKQNVKLIRINYWVEKIESGIHFDGNIVH 180

Query: 202  TDNQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQ 261
            TDNQ+RRARCWFPC+DD   RC +DLEFTV  N VAVS G LLYQV+ K++  +KTYVY+
Sbjct: 181  TDNQMRRARCWFPCIDDEYHRCSFDLEFTVPHNFVAVSVGKLLYQVMCKEDTTQKTYVYE 240

Query: 262  VDIPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSV 321
            + IP+  +W+SL  GP EIL D  N LIS++CLP +  +L+NT+EFFH A+S Y+DYLS 
Sbjct: 241  LAIPIAPRWVSLVAGPLEILPDQTNFLISNLCLPHDLSRLRNTMEFFHEAYSYYEDYLSA 300

Query: 322  DFPFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWF 381
            +FPFG YKQ+F+ PEM V+S+  G S+ IFSSH+L+DE++IDQTIDTRIKLA ALA+QWF
Sbjct: 301  NFPFGFYKQVFLPPEMVVTSSTSGASLSIFSSHILYDERVIDQTIDTRIKLASALAKQWF 360

Query: 382  GIYITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSS 441
            G+YITPE+PND+WLLDGLAGFLTD+ IK+ LGNNEARY+RYKANCAVCKADDSG   LSS
Sbjct: 361  GVYITPESPNDDWLLDGLAGFLTDMFIKQFLGNNEARYRRYKANCAVCKADDSGAMCLSS 420

Query: 442  SSACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRS 501
            S +C+D  G +++            A+LQMLEKQMG +SFRKILQ I+SRAKD +++ RS
Sbjct: 421  SPSCRDLFGTHSIGMHGKIRSWKSGAVLQMLEKQMGSDSFRKILQKIISRAKDPSNSIRS 480

Query: 502  LSTKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECT- 561
            LSTKE       IGNLERPFLKEF  RWV S GCP+LR+G SYNKRKN VEMA  RECT 
Sbjct: 481  LSTKEFRQFANKIGNLERPFLKEFFQRWVASYGCPVLRIGLSYNKRKNNVEMAALRECTA 540

Query: 562  -------VTPATS-VENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHS 621
                   V  ATS  E+RD D GWPG+MSIR+YELDG+ DHP LPM G+ WQLLE+ CHS
Sbjct: 541  ALDARLSVIGATSDSESRDVDAGWPGIMSIRVYELDGMSDHPKLPMAGDRWQLLELPCHS 600

Query: 622  KLAARRLQKTKKGSKPDGSDDNTDTPA-LDVRSSVESPLLWLRADPEMEYLAEIHFHQPV 681
            KLAA+R QK KKG KPDG++DN D  A L+ ++S+ESPL W++ADPEMEY+AEIH HQP+
Sbjct: 601  KLAAKRYQKPKKGGKPDGAEDNVDAIAPLENKTSIESPLAWIKADPEMEYIAEIHLHQPL 660

Query: 682  QMWINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAK 741
            QMW+NQLEKD DV+AQAQAI +LE L Q SFS+VNAL N LTD K FWR+RI AA A+AK
Sbjct: 661  QMWVNQLEKDGDVVAQAQAIASLEALKQHSFSIVNALKNVLTDSKVFWRIRIAAAFALAK 720

Query: 742  TASEDTDWAGLLNLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQK 801
            TASE++DWAGL +LIKF+KS+RFDA+ GLPKPNDFRDFPEYFVLEAIPHA+A+VR  + K
Sbjct: 721  TASEESDWAGLQHLIKFYKSRRFDAEIGLPKPNDFRDFPEYFVLEAIPHAIAIVRGAEGK 780

Query: 802  SPREAVEFVLQLLKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLL 861
            SPREAVEF+LQLLKYNDN+GN YSDVFWLA LVQSVG+LEF QQS+ FLA LLKRIDRLL
Sbjct: 781  SPREAVEFILQLLKYNDNSGNSYSDVFWLAVLVQSVGDLEFCQQSLTFLAPLLKRIDRLL 840

Query: 862  QFDRLMPSFNGILTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRA 921
            QFDRLMPS+NGILTISCIRTL Q ALKLS  +S D + +LI PFRN +++ Q+RIE +RA
Sbjct: 841  QFDRLMPSYNGILTISCIRTLAQTALKLSDSISFDHICKLIEPFRNSDTILQIRIEGSRA 900

Query: 922  LLDLEYHCKGIDAALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLV 981
            LLD+EY  KGI +ALLLF+KYL EE SLRGQVKL VH MRLCQI    DS+D V++ TL+
Sbjct: 901  LLDIEYQSKGISSALLLFMKYLVEESSLRGQVKLCVHTMRLCQIAVGCDSDDCVDTVTLL 960

Query: 982  ALLLLLEGHMAFNNVYLRHYLFCILQVLAGR----------------------------- 1041
             LL L + H+ FNN  LR+YLFCI Q+LAGR                             
Sbjct: 961  DLLHLFKSHVVFNNELLRYYLFCIFQILAGRPPTLFGVPKEKPLQLVDVEACIEPKNVFL 1020

Query: 1042 ---------------------------WVPISLNALYF----EVVFSSTCVGFVYDHHL- 1101
                                        VPI    ++     E++       +    HL 
Sbjct: 1021 VPGAEAGEPSLSALGDAKGQSLDVAPYGVPIIPQEMFMPIVPELMLPEPVAAYDETQHLE 1080

Query: 1102 AHADSFAIPEVSNEGGAIPEFPKLAMAIVE--APREA-------------ASVSNSHERK 1161
               +S   P  S+E   + E P       E  A REA              SVS SHE K
Sbjct: 1081 PRMESQNQP--SHENPIVHEIPSDVEGPTEELAHREANPPTKEPQKEPDVVSVSVSHEVK 1140

Query: 1162 LPVVKIKVRSSAATSRAEADNQTIERSH--AAPHETDVGPSSSVSVDAPQRNIAEATSIS 1221
              V++IKVR S ATSRAE   +TIERS      H+ D G +SS SVDAPQR   +A SIS
Sbjct: 1141 KSVIRIKVRPSGATSRAEGSARTIERSQGIVVRHDIDRGQTSSASVDAPQRISTDAVSIS 1200

Query: 1222 NQN-LEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTAD------SSRAFGNFHPE 1281
            NQN +EEVNSCHD GS MTASIGS K AS+GD  GK+ QCTA+      S +A  N    
Sbjct: 1201 NQNHVEEVNSCHDVGSRMTASIGSVKFASEGDIFGKELQCTAESGKPSTSQKADNNNRTV 1260

Query: 1282 DPSSSSIIQDNNVDADA-QKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1326
             PS   +  D++++ +A QKYASLQTLS+ +             +KEKKKD+EKK K   
Sbjct: 1261 PPSFLPL--DHSMENEAQQKYASLQTLSIGK-------------EKEKKKDKEKKEK--- 1320

BLAST of Sgr027459 vs. TAIR 10
Match: AT1G73960.2 (TBP-associated factor 2 )

HSP 1 Score: 1420.6 bits (3676), Expect = 0.0e+00
Identity = 794/1420 (55.92%), Postives = 967/1420 (68.10%), Query Frame = 0

Query: 22   MAKPRKPKNTE--DTKPPDNSGAVVRHQKLCLSIDIDNRRIYGFTELEIAVPDIGIAGLH 81
            MAK RKPKN E    K  +N+GA V HQKL LSID   R+IYG+TELE++VPDIGI GLH
Sbjct: 1    MAKARKPKNEEAPGAKTSENTGAKVLHQKLFLSIDFKKRQIYGYTELEVSVPDIGIVGLH 60

Query: 82   AENLGIVSVSVDGDPTEFEYYPRHQHVESEKSFKAVSSPSSAADAAGSVYLSSIEKELVP 141
            AENLGI SV VDG+PT FEYYP HQ+ E+E ++ +VS P+SAADAA   Y+  +++E   
Sbjct: 61   AENLGIESVLVDGEPTVFEYYPHHQNSETESNWNSVSDPASAADAAAMEYVGVLKREDTA 120

Query: 142  NLLINCCKAFKSGSEQQEQPFLENGVQSAGEDKQNIRLVRIDYWVEKSEVGIHFSNHMAH 201
            NLLINCCK  K  SEQ +   LENG QS+GE KQN++L+RI+YWVEK E GIHF  ++ H
Sbjct: 121  NLLINCCKPSKDLSEQLDSVTLENGSQSSGEAKQNVKLIRINYWVEKIESGIHFDGNIVH 180

Query: 202  TDNQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKTYVYQ 261
            TDNQ+RRARCWFPC+DD   RC +DLEFTV  N VAVS G LLYQV+ K++  +KTYVY+
Sbjct: 181  TDNQMRRARCWFPCIDDEYHRCSFDLEFTVPHNFVAVSVGKLLYQVMCKEDTTQKTYVYE 240

Query: 262  VDIPVNAQWISLAVGPFEILADHQNGLISHMCLPVNSLKLKNTVEFFHSAFSCYKDYLSV 321
            + IP+  +W+SL  GP EIL D  N LIS++CLP +  +L+NT+EFFH A+S Y+DYLS 
Sbjct: 241  LAIPIAPRWVSLVAGPLEILPDQTNFLISNLCLPHDLSRLRNTMEFFHEAYSYYEDYLSA 300

Query: 322  DFPFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIKLAYALARQWF 381
            +FPFG YKQ+F+ PEM V+S+  G S+ IFSSH+L+DE++IDQTIDTRIKLA ALA+QWF
Sbjct: 301  NFPFGFYKQVFLPPEMVVTSSTSGASLSIFSSHILYDERVIDQTIDTRIKLASALAKQWF 360

Query: 382  GIYITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKADDSGLTTLSS 441
            G+YITPE+PND+WLLDGLAGFLTD+ IK+ LGNNEARY+RYKANCAVCKADDSG   LSS
Sbjct: 361  GVYITPESPNDDWLLDGLAGFLTDMFIKQFLGNNEARYRRYKANCAVCKADDSGAMCLSS 420

Query: 442  SSACKDCMGPNAL-----------VAILQMLEKQMGPESFRKILQNIVSRAKDTTSTSRS 501
            S +C+D  G +++            A+LQMLEKQMG +SFRKILQ I+SRAKD +++ RS
Sbjct: 421  SPSCRDLFGTHSIGMHGKIRSWKSGAVLQMLEKQMGSDSFRKILQKIISRAKDPSNSIRS 480

Query: 502  LSTKE-------IGNLERPFLKEFIPRWVESCGCPLLRMGFSYNKRKNMVEMAVSRECT- 561
            LSTKE       IGNLERPFLKEF  RWV S GCP+LR+G SYNKRKN VEMA  RECT 
Sbjct: 481  LSTKEFRQFANKIGNLERPFLKEFFQRWVASYGCPVLRIGLSYNKRKNNVEMAALRECTA 540

Query: 562  -------VTPATS-VENRDSDTGWPGMMSIRIYELDGVFDHPVLPMNGESWQLLEIQCHS 621
                   V  ATS  E+RD D GWPG+MSIR+YELDG+ DHP LPM G+ WQLLE+ CHS
Sbjct: 541  ALDARLSVIGATSDSESRDVDAGWPGIMSIRVYELDGMSDHPKLPMAGDRWQLLELPCHS 600

Query: 622  KLAARRLQKTKKGSKPDGSDDNTDTPA-LDVRSSVESPLLWLRADPEMEYLAEIHFHQPV 681
            KLAA+R QK KKG KPDG++DN D  A L+ ++S+ESPL W++ADPEMEY+AEIH HQP+
Sbjct: 601  KLAAKRYQKPKKGGKPDGAEDNVDAIAPLENKTSIESPLAWIKADPEMEYIAEIHLHQPL 660

Query: 682  QMWINQLEKDKDVIAQAQAIMTLEMLPQPSFSVVNALNNFLTDPKAFWRVRIDAALAMAK 741
            QMW+NQLEKD DV+AQAQAI +LE L Q SFS+VNAL N LTD K FWR+RI AA A+AK
Sbjct: 661  QMWVNQLEKDGDVVAQAQAIASLEALKQHSFSIVNALKNVLTDSKVFWRIRIAAAFALAK 720

Query: 742  TASEDTDWAGLLNLIKFFKSQRFDADTGLPKPNDFRDFPEYFVLEAIPHAVAMVRATDQK 801
            TASE++DWAGL +LIKF+KS+RFDA+ GLPKPNDFRDFPEYFVLEAIPHA+A+VR  + K
Sbjct: 721  TASEESDWAGLQHLIKFYKSRRFDAEIGLPKPNDFRDFPEYFVLEAIPHAIAIVRGAEGK 780

Query: 802  SPREAVEFVLQLLKYNDNNGNPYSDVFWLAALVQSVGELEFGQQSILFLASLLKRIDRLL 861
            SPREAVEF+LQLLKYNDN+GN YSDVFWLA LVQSVG+LEF QQS+ FLA LLKRIDRLL
Sbjct: 781  SPREAVEFILQLLKYNDNSGNSYSDVFWLAVLVQSVGDLEFCQQSLTFLAPLLKRIDRLL 840

Query: 862  QFDRLMPSFNGILTISCIRTLTQIALKLSGLLSLDRVFELIRPFRNFNSMWQVRIEATRA 921
            QFDRLMPS+NGILTISCIRTL Q ALKLS  +S D + +LI PFRN +++ Q+RIE +RA
Sbjct: 841  QFDRLMPSYNGILTISCIRTLAQTALKLSDSISFDHICKLIEPFRNSDTILQIRIEGSRA 900

Query: 922  LLDLEYHCKGIDAALLLFIKYLEEEKSLRGQVKLGVHVMRLCQIMRRSDSNDLVNSDTLV 981
            LLD+EY  K                    GQVKL VH MRLCQI    DS+D V++ TL+
Sbjct: 901  LLDIEYQSK--------------------GQVKLCVHTMRLCQIAVGCDSDDCVDTVTLL 960

Query: 982  ALLLLLEGHMAFNNVYLRHYLFCILQVLAGR----------------------------- 1041
             LL L + H+ FNN  LR+YLFCI Q+LAGR                             
Sbjct: 961  DLLHLFKSHVVFNNELLRYYLFCIFQILAGRPPTLFGVPKEKPLQLVDVEACIEPKNVFL 1020

Query: 1042 ---------------------------WVPISLNALYF----EVVFSSTCVGFVYDHHL- 1101
                                        VPI    ++     E++       +    HL 
Sbjct: 1021 VPGAEAGEPSLSALGDAKGQSLDVAPYGVPIIPQEMFMPIVPELMLPEPVAAYDETQHLE 1080

Query: 1102 AHADSFAIPEVSNEGGAIPEFPKLAMAIVE--APREA-------------ASVSNSHERK 1161
               +S   P  S+E   + E P       E  A REA              SVS SHE K
Sbjct: 1081 PRMESQNQP--SHENPIVHEIPSDVEGPTEELAHREANPPTKEPQKEPDVVSVSVSHEVK 1140

Query: 1162 LPVVKIKVRSSAATSRAEADNQTIERSH--AAPHETDVGPSSSVSVDAPQRNIAEATSIS 1221
              V++IKVR S ATSRAE   +TIERS      H+ D G +SS SVDAPQR   +A SIS
Sbjct: 1141 KSVIRIKVRPSGATSRAEGSARTIERSQGIVVRHDIDRGQTSSASVDAPQRISTDAVSIS 1200

Query: 1222 NQN-LEEVNSCHDQGSHMTASIGSAKLASDGDELGKDFQCTAD------SSRAFGNFHPE 1281
            NQN +EEVNSCHD GS MTASIGS K AS+GD  GK+ QCTA+      S +A  N    
Sbjct: 1201 NQNHVEEVNSCHDVGSRMTASIGSVKFASEGDIFGKELQCTAESGKPSTSQKADNNNRTV 1260

Query: 1282 DPSSSSIIQDNNVDADA-QKYASLQTLSLPQHDHGLASLQSRHGKKEKKKDREKKRKRES 1326
             PS   +  D++++ +A QKYASLQTLS+ +             +KEKKKD+EKK K   
Sbjct: 1261 PPSFLPL--DHSMENEAQQKYASLQTLSIGK-------------EKEKKKDKEKKEK--- 1320

BLAST of Sgr027459 vs. TAIR 10
Match: AT4G33090.1 (aminopeptidase M1 )

HSP 1 Score: 67.0 bits (162), Expect = 1.3e-10
Identity = 76/336 (22.62%), Postives = 143/336 (42.56%), Query Frame = 0

Query: 196 HMAHTDNQIRRARCWFPCMDDGLQRCKYDLEFTVSQNLVAVSNGMLLYQVLSKDNPPRKT 255
           +MA T  +   AR  FPC D+   +  + +   V  +LVA+SN  ++ +   K N   K 
Sbjct: 131 NMAVTQFEPADARRCFPCWDEPACKATFKITLEVPTDLVALSNMPIMEE---KVNGNLKI 190

Query: 256 YVYQVDIPVNAQWISLAVGPFEILADH-QNGL-ISHMCLPVNSLKLKNTVEFFHSAFSCY 315
             YQ    ++   +++ VG F+ + DH  +G+ +   C    + + K  +         +
Sbjct: 191 VSYQESPIMSTYLVAIVVGLFDYVEDHTSDGIKVRVYCQVGKADQGKFALHVGAKTLDLF 250

Query: 316 KDYLSVDFPFGSYKQIFIEPEMAVSSACLGVSMCIFSSHLLFDEKIIDQTIDTRIK--LA 375
           K+Y +V +P      I I P+ A  +      +    + LL+DE+    +   R+   +A
Sbjct: 251 KEYFAVPYPLPKMDMIAI-PDFAAGAMENYGLVTYRETALLYDEQHSAASNKQRVATVVA 310

Query: 376 YALARQWFGIYITPETPNDEWLLDGLAGFLTDLVIKKNLGNNEARYQRYKANCAVCKAD- 435
           + LA QWFG  +T E     WL +G A +++ L         +   Q    +    + D 
Sbjct: 311 HELAHQWFGNLVTMEWWTHLWLNEGFATWVSYLATDSLFPEWKIWTQFLDESTEGLRLDG 370

Query: 436 --DSGLTTLSSSSACK-----DCMGPNALVAILQMLEKQMGPESFRKIL-QNIVSRAKDT 495
             +S    +  + A +     D +      ++++ML+  +G E F+K L   I + A   
Sbjct: 371 LEESHPIEVEVNHAAEIDEIFDAISYRKGASVIRMLQSYLGAEVFQKSLAAYIKNHAYSN 430

Query: 496 TSTSRSLSTKEIGNLERPFLKEFIPRWVESCGCPLL 519
             T    +  E G+ E   + + +  W +  G P++
Sbjct: 431 AKTEDLWAALEAGSGEP--VNKLMSSWTKQKGYPVV 460