Sgr027415 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr027415
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionAuxin efflux carrier component
Locationtig00153054: 1239811 .. 1242849 (+)
RNA-Seq ExpressionSgr027415
SyntenySgr027415
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGCGGTGGTTCCTCTTTACGTCGCCATGATCTTAGCTTACGGCTCTGTTAAGTGGTGGAAGATCTTCACTCCCGACCAGTGCTCTGGAATCAATCGCTTCGTCGCTTTGTTTGCTGTCCCTCTCCTCTCTTTTCACTTCATCTCCACCAACAATCCCTACACCATGAACCTGCGCTTCATCGCCGCAGACACTCTCCAAAAACTTATCGTTCTTGCTGTTCTTGCCGTCTGGAGCAACGTGAGCAAGAGGGGGTGTTTGGAATGGACGATTACTCTGTTTTCTCTTTCCACTCTGCCCAACACTCTGGTTATGGGTATCCCTCTGCTCAAAGGAATGTATGGAGAGTTCTCTGGGAGCTTGATGGTTCAGATTGTTGTGCTTCAGTGCATTATTTGGTACACATTGATGCTGTTCATGTTTGAATACAGAGGAGCCAGAATGCTGATCTCTGAGCAGTTTCCAGACACTGCTGGTTCTATAGTCTCCATCCATGTCGACTCTGACATTATGTCATTAGATGGAAGACAAGTTTTGGAAACAGAAGCTGAGATCAAAGAAGATGGGAAGCTTCACGTGACGGTGAGGAAATCAAATGCCTCAAGATCAGATATCTTTTCGAGAAGATCTGTGGGGTTGTCTTCTACAACGCCGAGGCCTTCGAATCTGACTAATGCAGAGATTTACTCTCTGCAATCTTCTCGCAACCCAACTCCCAGAGGCTCTAGTTTCAACCACACGGATTTCTACTCAATGATGGCGGCCGGCGGGAGAAACTCCAACTTCGGCTCCTCGGACGTCTATGGCCTCTCCGCCTCGAGAGGGCCGACTCCGAGACCGTCTAACTACGAGGAAGACGGCGGAGCTAACGGCGGAAAGCCGAGGTTCCATTACAATGCCACAGCGGGAGGCAATGCAACGCATTACCCTGCGCCAAACCCAGGAATGTTTTCGCCTACAGGTTCCAAAAATGCACCTTCGAACACGAAGAAGCCCGCCAATGGCGTCGTGGCTCAGCAGAAGCCAGAGGATGGAAGTAGAGACCTGCACATGTTTGTTTGGAGTTCAAGCGCCTCCCCTGTCTCAGACGTATTTGGAAACCATGAATTTGGCGGACACCATGATCAGAAGGAAGTGAGATTGGCTGTCTCCCCCGGAAAAGGTAACTTTCTCGAACAACATAGCTTCTGTATCCACTTTGTTTTCGTTAGTTCTTCCATTTTCGTTAATGGGGTTTCGATTTTTTAGTCGAGGGTCGTAGAGAAAACCAAGAAGAGTACGCAGAGAGAGAAGATTTCAGCTTCGGAAACAGAGAGATGATGAACAACAACAATGGAGCAGAGAAAGTTGGAGATGCCAAACCCAAAACCATGCCACCCACCAGCGTCATGACAAGGCTCATCCTCATCATGGTATGGAGAAAACTCATCAGAAACCCCAACACTTACTCCAGCTTAATTGGCCTCACTTGGTCCCTAGTCTCATTCAGGTTCGCCAAATTCACATTCTCCATCCTTTTTTTTTTCTCAAAACCCCATTTGGTATTATTAACTTTGATTCTGATTATTATCTTTGTCTTAAGGTGGAACGTTGATATGCCAGCCATTGTTGCAAAGTCCATTTCAATACTATCAGATGCAGGGCTTGGCATGGCCATGTTTAGTCTTGGCAAGTTCTCGACATCCATTTTTCTTCGTCTTCCTTTTTCTCTTCTTCTTACCCTTTATTTGCTGTTTATTCTTCCCTTACGCCTATCATCATCAAAAAGCAAGCAAAAACTGGCACAAGGCAAAGCAAAACAGTGGCATTGTTATGATATTAAGTTGGGTTTCTGTTGATCGCACACTCCCCCCCCCCCCCTCCTCTGCTCTTTCTCTCTCTTTCACTTTTCTGCCTGCTCTGTGTTGGATTCGATTCAAATGGGTTGGTGGTGGTGGTGGTGATGATGAAAGTTGCTTTAAGGAATAAAAAAAAGAGGAGAGAGAGAGAGAGGGAAAAGAGAGAGAAAGCATGCTAAATGGTGTTTGCTGTAAATGACAGGTTTGTTCATGGCTTTGCAACCAAGGATCATAGCATGTGGAAATACCATAGCAGCTTTTTCAATGGCTGTGAGATTCCTTACAGGTCCAGCTGTAATGGCTGTTGCTTCCATTGCTGTTGGCCTTAGAGGAGTTCTCTTACGTGTTGCCATTGTCCAGGTCACCCTTTCTCTCTCTACCTCTCTCTCTGTTCTGCATCTCTCTGGCACTACCCTATCCTGCTTCACTGCATATACTCCAAAAAGTTGCTCTTTAAAACCAATATATATATATATATATGTAAAACTTAAAAACCCCCCTTTATTTTCTCTATCTCTCTCAGCAATAAAGCTGTTTAATATATACATATCCAAATGGGTAATGAAAAAGACTGTTTCTTTTTTGAGTTAAAAGGAGTGAAAACTTGTGTAATGGGGTGTGGGTTAGTTATATTATAACTTACAAGGGTTTATAAGCTAATAGGCCTAAGGACCTGTTTGGTGATATGTTAGGAAACAAACATCTGTGGCTCTGTTTAACATGTTTTTTTGCCCCATGTGTTTGGATTTTCCAAACACAACACATCTAAAACAAAAGAAATTGTTGAACACAGGCAGCTCTCCCACAAGGAATTGTCCCCTTTGTCTTTGCCAAGGAGTACAACGTACACCCTGATATTCTCAGCACAGGGTATGTAGTAGAAATGTCTTAATAACTAAATAAAAATGTGTTTTTTTAAAAAAAGTTCTCTAATTTTGCTTCTTTTTTTTTCTGGGTTTGCTGCAGAGTCATTTTTGGGATGTTGATTGCTTTGCCCATCACTCTTGTGTACTACATTTTGCTGGGGATCTGAAGCTGTGGAAGCAACTCAGCCATGGCTGCGGCCGCCATGAGAGGGCAGTTTCAGAAGCTTCAAAATGGCTTTGGGAAAGAAGATTTTAGAGAGGGAGAAAGAGAAGAGAGAAGATGGGGGAAGTCAAATGGAGGAGGAAGAGGAGAGATGAGGAATTGGAGATGA

mRNA sequence

ATGACGGCGGTGGTTCCTCTTTACGTCGCCATGATCTTAGCTTACGGCTCTGTTAAGTGGTGGAAGATCTTCACTCCCGACCAGTGCTCTGGAATCAATCGCTTCGTCGCTTTGTTTGCTGTCCCTCTCCTCTCTTTTCACTTCATCTCCACCAACAATCCCTACACCATGAACCTGCGCTTCATCGCCGCAGACACTCTCCAAAAACTTATCGTTCTTGCTGTTCTTGCCGTCTGGAGCAACGTGAGCAAGAGGGGGTGTTTGGAATGGACGATTACTCTGTTTTCTCTTTCCACTCTGCCCAACACTCTGGTTATGGGTATCCCTCTGCTCAAAGGAATGTATGGAGAGTTCTCTGGGAGCTTGATGGTTCAGATTGTTGTGCTTCAGTGCATTATTTGGTACACATTGATGCTGTTCATGTTTGAATACAGAGGAGCCAGAATGCTGATCTCTGAGCAGTTTCCAGACACTGCTGGTTCTATAGTCTCCATCCATGTCGACTCTGACATTATGTCATTAGATGGAAGACAAGTTTTGGAAACAGAAGCTGAGATCAAAGAAGATGGGAAGCTTCACGTGACGGTGAGGAAATCAAATGCCTCAAGATCAGATATCTTTTCGAGAAGATCTGTGGGGTTGTCTTCTACAACGCCGAGGCCTTCGAATCTGACTAATGCAGAGATTTACTCTCTGCAATCTTCTCGCAACCCAACTCCCAGAGGCTCTAGTTTCAACCACACGGATTTCTACTCAATGATGGCGGCCGGCGGGAGAAACTCCAACTTCGGCTCCTCGGACGTCTATGGCCTCTCCGCCTCGAGAGGGCCGACTCCGAGACCGTCTAACTACGAGGAAGACGGCGGAGCTAACGGCGGAAAGCCGAGGTTCCATTACAATGCCACAGCGGGAGGCAATGCAACGCATTACCCTGCGCCAAACCCAGGAATGTTTTCGCCTACAGGTTCCAAAAATGCACCTTCGAACACGAAGAAGCCCGCCAATGGCGTCGTGGCTCAGCAGAAGCCAGAGGATGGAAGTAGAGACCTGCACATGTTTGTTTGGAGTTCAAGCGCCTCCCCTGTCTCAGACGTATTTGGAAACCATGAATTTGGCGGACACCATGATCAGAAGGAAGTGAGATTGGCTGTCTCCCCCGGAAAAGTCGAGGGTCGTAGAGAAAACCAAGAAGAGTACGCAGAGAGAGAAGATTTCAGCTTCGGAAACAGAGAGATGATGAACAACAACAATGGAGCAGAGAAAGTTGGAGATGCCAAACCCAAAACCATGCCACCCACCAGCGTCATGACAAGGCTCATCCTCATCATGGTATGGAGAAAACTCATCAGAAACCCCAACACTTACTCCAGCTTAATTGGCCTCACTTGGTCCCTAGTCTCATTCAGGTGGAACGTTGATATGCCAGCCATTGTTGCAAAGTCCATTTCAATACTATCAGATGCAGGGCTTGGCATGGCCATGTTTAGTCTTGGTTTGTTCATGGCTTTGCAACCAAGGATCATAGCATGTGGAAATACCATAGCAGCTTTTTCAATGGCTGTGAGATTCCTTACAGGTCCAGCTGTAATGGCTGTTGCTTCCATTGCTGTTGGCCTTAGAGGAGTTCTCTTACGTGTTGCCATTGTCCAGGCAGCTCTCCCACAAGGAATTGTCCCCTTTGTCTTTGCCAAGGAGTACAACGTACACCCTGATATTCTCAGCACAGGCTGTGGAAGCAACTCAGCCATGGCTGCGGCCGCCATGAGAGGGCAGTTTCAGAAGCTTCAAAATGGCTTTGGGAAAGAAGATTTTAGAGAGGGAGAAAGAGAAGAGAGAAGATGGGGGAAGTCAAATGGAGGAGGAAGAGGAGAGATGAGGAATTGGAGATGA

Coding sequence (CDS)

ATGACGGCGGTGGTTCCTCTTTACGTCGCCATGATCTTAGCTTACGGCTCTGTTAAGTGGTGGAAGATCTTCACTCCCGACCAGTGCTCTGGAATCAATCGCTTCGTCGCTTTGTTTGCTGTCCCTCTCCTCTCTTTTCACTTCATCTCCACCAACAATCCCTACACCATGAACCTGCGCTTCATCGCCGCAGACACTCTCCAAAAACTTATCGTTCTTGCTGTTCTTGCCGTCTGGAGCAACGTGAGCAAGAGGGGGTGTTTGGAATGGACGATTACTCTGTTTTCTCTTTCCACTCTGCCCAACACTCTGGTTATGGGTATCCCTCTGCTCAAAGGAATGTATGGAGAGTTCTCTGGGAGCTTGATGGTTCAGATTGTTGTGCTTCAGTGCATTATTTGGTACACATTGATGCTGTTCATGTTTGAATACAGAGGAGCCAGAATGCTGATCTCTGAGCAGTTTCCAGACACTGCTGGTTCTATAGTCTCCATCCATGTCGACTCTGACATTATGTCATTAGATGGAAGACAAGTTTTGGAAACAGAAGCTGAGATCAAAGAAGATGGGAAGCTTCACGTGACGGTGAGGAAATCAAATGCCTCAAGATCAGATATCTTTTCGAGAAGATCTGTGGGGTTGTCTTCTACAACGCCGAGGCCTTCGAATCTGACTAATGCAGAGATTTACTCTCTGCAATCTTCTCGCAACCCAACTCCCAGAGGCTCTAGTTTCAACCACACGGATTTCTACTCAATGATGGCGGCCGGCGGGAGAAACTCCAACTTCGGCTCCTCGGACGTCTATGGCCTCTCCGCCTCGAGAGGGCCGACTCCGAGACCGTCTAACTACGAGGAAGACGGCGGAGCTAACGGCGGAAAGCCGAGGTTCCATTACAATGCCACAGCGGGAGGCAATGCAACGCATTACCCTGCGCCAAACCCAGGAATGTTTTCGCCTACAGGTTCCAAAAATGCACCTTCGAACACGAAGAAGCCCGCCAATGGCGTCGTGGCTCAGCAGAAGCCAGAGGATGGAAGTAGAGACCTGCACATGTTTGTTTGGAGTTCAAGCGCCTCCCCTGTCTCAGACGTATTTGGAAACCATGAATTTGGCGGACACCATGATCAGAAGGAAGTGAGATTGGCTGTCTCCCCCGGAAAAGTCGAGGGTCGTAGAGAAAACCAAGAAGAGTACGCAGAGAGAGAAGATTTCAGCTTCGGAAACAGAGAGATGATGAACAACAACAATGGAGCAGAGAAAGTTGGAGATGCCAAACCCAAAACCATGCCACCCACCAGCGTCATGACAAGGCTCATCCTCATCATGGTATGGAGAAAACTCATCAGAAACCCCAACACTTACTCCAGCTTAATTGGCCTCACTTGGTCCCTAGTCTCATTCAGGTGGAACGTTGATATGCCAGCCATTGTTGCAAAGTCCATTTCAATACTATCAGATGCAGGGCTTGGCATGGCCATGTTTAGTCTTGGTTTGTTCATGGCTTTGCAACCAAGGATCATAGCATGTGGAAATACCATAGCAGCTTTTTCAATGGCTGTGAGATTCCTTACAGGTCCAGCTGTAATGGCTGTTGCTTCCATTGCTGTTGGCCTTAGAGGAGTTCTCTTACGTGTTGCCATTGTCCAGGCAGCTCTCCCACAAGGAATTGTCCCCTTTGTCTTTGCCAAGGAGTACAACGTACACCCTGATATTCTCAGCACAGGCTGTGGAAGCAACTCAGCCATGGCTGCGGCCGCCATGAGAGGGCAGTTTCAGAAGCTTCAAAATGGCTTTGGGAAAGAAGATTTTAGAGAGGGAGAAAGAGAAGAGAGAAGATGGGGGAAGTCAAATGGAGGAGGAAGAGGAGAGATGAGGAATTGGAGATGA

Protein sequence

MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLRFIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVLETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHYNATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRDLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMMNNNNGAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTGCGSNSAMAAAAMRGQFQKLQNGFGKEDFREGEREERRWGKSNGGGRGEMRNWR
Homology
BLAST of Sgr027415 vs. NCBI nr
Match: XP_038896073.1 (probable auxin efflux carrier component 1c isoform X2 [Benincasa hispida])

HSP 1 Score: 1062.8 bits (2747), Expect = 1.2e-306
Identity = 556/586 (94.88%), Postives = 562/586 (95.90%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEED-GGANGGKPRFHY 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+ GG NGGKPRFHY
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGNGGKPRFHY 310

Query: 301 NATAGG------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRDLHMF 360
           NAT GG      NA HYPAPNPGMFSPTGSKNA  N KKPANGVV QQK EDGSRDLHMF
Sbjct: 311 NATTGGNANANANANHYPAPNPGMFSPTGSKNAQPNAKKPANGVVGQQKTEDGSRDLHMF 370

Query: 361 VWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM 420
           VWSSSASPVSDVFGNHEFG H DQK+VRLAVSPGKVE RRENQEEYAEREDFSFGNREMM
Sbjct: 371 VWSSSASPVSDVFGNHEFGAHTDQKDVRLAVSPGKVEVRRENQEEYAEREDFSFGNREMM 430

Query: 421 NNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRW 480
           N+NN   G EKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRW
Sbjct: 431 NSNNNGGGTEKVGDIKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRW 490

Query: 481 NVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVM 540
           NV+MPAI+AKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVM
Sbjct: 491 NVEMPAIIAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVM 550

Query: 541 AVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           AVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 AVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 596

BLAST of Sgr027415 vs. NCBI nr
Match: KAG6584319.1 (Auxin efflux carrier component 1a, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1062.4 bits (2746), Expect = 1.5e-306
Identity = 556/593 (93.76%), Postives = 565/593 (95.28%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 60

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 61  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 120

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGA------NGGK 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG       NGGK
Sbjct: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGGGGGNNGGK 300

Query: 301 PRFHYNATAGG--------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDG 360
           PRFHYNAT GG        NA HYPAPNPGMFSPTGSKNA  NTKKPANG VAQ K EDG
Sbjct: 301 PRFHYNATTGGTVNANANANANHYPAPNPGMFSPTGSKNAQPNTKKPANGAVAQPKTEDG 360

Query: 361 SRDLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFS 420
           SRDLHMFVWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAEREDFS
Sbjct: 361 SRDLHMFVWSSSASPVSDVFGNHEFGAHNDQKDVRLAVSPGKVEGRRENQEEYAEREDFS 420

Query: 421 FGNREMMNNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTW 480
           FGNRE+MN+NN   GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTW
Sbjct: 421 FGNREIMNSNNNNGGAEKVGDVKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTW 480

Query: 481 SLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRF 540
           SLVSFRWNV+MPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMAVRF
Sbjct: 481 SLVSFRWNVEMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMAVRF 540

Query: 541 LTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           LTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 541 LTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 593

BLAST of Sgr027415 vs. NCBI nr
Match: XP_022923713.1 (probable auxin efflux carrier component 1c [Cucurbita moschata])

HSP 1 Score: 1061.2 bits (2743), Expect = 3.4e-306
Identity = 555/591 (93.91%), Postives = 564/591 (95.43%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGA------NGGK 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG       NGGK
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGGGGGNNGGK 310

Query: 301 PRFHYNATAGG------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSR 360
           PRFHYNAT GG      NA HYPAPNPGMFSPTGSKNA  N KKPANG VAQ K EDGSR
Sbjct: 311 PRFHYNATTGGTVNANANANHYPAPNPGMFSPTGSKNAQPNAKKPANGAVAQPKTEDGSR 370

Query: 361 DLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFG 420
           DLHMFVWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAEREDFSFG
Sbjct: 371 DLHMFVWSSSASPVSDVFGNHEFGAHNDQKDVRLAVSPGKVEGRRENQEEYAEREDFSFG 430

Query: 421 NREMMNNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL 480
           NRE+MN+NN   GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL
Sbjct: 431 NREIMNSNNNNGGAEKVGDVKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL 490

Query: 481 VSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLT 540
           VSFRWNV+MPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMAVRFLT
Sbjct: 491 VSFRWNVEMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMAVRFLT 550

Query: 541 GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 601

BLAST of Sgr027415 vs. NCBI nr
Match: XP_023519167.1 (probable auxin efflux carrier component 1c [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1059.7 bits (2739), Expect = 1.0e-305
Identity = 555/595 (93.28%), Postives = 564/595 (94.79%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGA------NGGK 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG       NGGK
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGGGGGNNGGK 310

Query: 301 PRFHYNATAGG----------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPE 360
           PRFHYNAT GG          NA HYPAPNPGMFSPTGSKNA  N KKPANG VAQ K E
Sbjct: 311 PRFHYNATTGGTVNANANANANANHYPAPNPGMFSPTGSKNAQPNAKKPANGAVAQPKTE 370

Query: 361 DGSRDLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAERED 420
           DGSRDLHMFVWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAERED
Sbjct: 371 DGSRDLHMFVWSSSASPVSDVFGNHEFGAHNDQKDVRLAVSPGKVEGRRENQEEYAERED 430

Query: 421 FSFGNREMMNNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGL 480
           FSFGNRE+MN+NN   GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGL
Sbjct: 431 FSFGNREIMNSNNNNGGAEKVGDVKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGL 490

Query: 481 TWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAV 540
           TWSLVSFRWNV+MPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMAV
Sbjct: 491 TWSLVSFRWNVEMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMAV 550

Query: 541 RFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           RFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 RFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 605

BLAST of Sgr027415 vs. NCBI nr
Match: XP_023001099.1 (probable auxin efflux carrier component 1c [Cucurbita maxima])

HSP 1 Score: 1058.5 bits (2736), Expect = 2.2e-305
Identity = 555/596 (93.12%), Postives = 564/596 (94.63%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGA---NGGKPRF 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG    NGGKPRF
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGGNNGGKPRF 310

Query: 301 HYNATAGG--------------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKP 360
           HYNAT GG              NA HYPAPNPGMFSPTGSKNA  N KKPANG VAQ K 
Sbjct: 311 HYNATTGGTVNANANANANANANANHYPAPNPGMFSPTGSKNAQPNAKKPANGAVAQPKT 370

Query: 361 EDGSRDLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAERE 420
           EDGSRDLHMFVWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAERE
Sbjct: 371 EDGSRDLHMFVWSSSASPVSDVFGNHEFGTHNDQKDVRLAVSPGKVEGRRENQEEYAERE 430

Query: 421 DFSFGNREMMNNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIG 480
           DFSFGNRE+MN+NN   GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIG
Sbjct: 431 DFSFGNREIMNSNNNNGGAEKVGDVKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIG 490

Query: 481 LTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMA 540
           LTWSLVSFRWNV+MPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMA
Sbjct: 491 LTWSLVSFRWNVEMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMA 550

Query: 541 VRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           VRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 VRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 606

BLAST of Sgr027415 vs. ExPASy Swiss-Prot
Match: Q9C6B8 (Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV=1)

HSP 1 Score: 806.2 bits (2081), Expect = 2.6e-232
Identity = 444/604 (73.51%), Postives = 491/604 (81.29%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTA+VPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFI+ NNPY MNLR
Sbjct: 11  MTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIAANNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           F+AAD+LQK+IVL++L +W  +S+ G L+WTITLFSLSTLPNTLVMGIPLLKGMYG FSG
Sbjct: 71  FLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPLLKGMYGNFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
            LMVQIVVLQCIIWYTLMLF+FEYRGA++LISEQFPDTAGSIVSIHVDSDIMSLDGRQ L
Sbjct: 131 DLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSDIMSLDGRQPL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVR+SNASRSDI+SRRS GLS+ TPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSA-TPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAA-GGRNSNFGSSD-VYGLSASRGPTPRPSNYEEDG--------GA 300
           RGSSFNHTDFYSMMA+ GGRNSNFG  + V+G   S+GPTPRPSNYEEDG        G 
Sbjct: 251 RGSSFNHTDFYSMMASGGGRNSNFGPGEAVFG---SKGPTPRPSNYEEDGGPAKPTAAGT 310

Query: 301 NGGKPRFHYNA--TAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGS- 360
             G  RFHY +  + GG   HYPAPNPGMFSP  +      T    N  V   K +DG+ 
Sbjct: 311 AAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSP--NTGGGGGTAAKGNAPVVGGKRQDGNG 370

Query: 361 RDLHMFVWSSSASPVSDVFGNHEFGGHH---------DQKEVRLAVSPGKVEGRRENQEE 420
           RDLHMFVWSSSASPVSDVFG    G HH          QK+V+++V  G       N  +
Sbjct: 371 RDLHMFVWSSSASPVSDVFGGGG-GNHHADYSTATNDHQKDVKISVPQG-----NSNDNQ 430

Query: 421 YAEREDFSFGNRE-------MMNNNNGAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRN 480
           Y ERE+FSFGN++           NN + K   A  K MPPTSVMTRLILIMVWRKLIRN
Sbjct: 431 YVEREEFSFGNKDDDSKVLATDGGNNISNKTTQA--KVMPPTSVMTRLILIMVWRKLIRN 490

Query: 481 PNTYSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACG 540
           PN+YSSL G+TWSL+SF+WN++MPA++AKSISILSDAGLGMAMFSLGLFMAL PRIIACG
Sbjct: 491 PNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFSLGLFMALNPRIIACG 550

Query: 541 NTIAAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPD 576
           N  AAF+ A+RF+ GPAVM VAS AVGLRGVLL VAI+QAALPQGIVPFVFAKEYNVHPD
Sbjct: 551 NRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQAALPQGIVPFVFAKEYNVHPD 600

BLAST of Sgr027415 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 8.7e-228
Identity = 442/596 (74.16%), Postives = 482/596 (80.87%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTA+VPLYVAMILAYGSVKWW+IFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR
Sbjct: 11  MTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLA+L +WS++S+RG LEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG
Sbjct: 71  FIAADTLQKLIVLALLTLWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQ-V 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGAR+LI+EQFPDTAG+I SI VD+D++SLDGR+ +
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFPDTAGAIASIVVDADVVSLDGRRDM 190

Query: 181 LETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPT 240
           +ETEAE+KEDGK+HVTVR+SNASRSD++SRRS+G SSTTPRPSNLTNAEIYSLQSSRNPT
Sbjct: 191 IETEAEVKEDGKIHVTVRRSNASRSDVYSRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPT 250

Query: 241 PRGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           PRGSSFNHTDFYSM+   GR+SNF + D +G+    G TPRPSNYEED  A         
Sbjct: 251 PRGSSFNHTDFYSMV---GRSSNFAAGDAFGVRT--GATPRPSNYEEDAAAPN------- 310

Query: 301 NATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRDLHMFVWSSSA 360
              AG     YPAPNP M        AP   KK ANG   Q K EDG +DLHMFVWSSSA
Sbjct: 311 --KAGSKYGQYPAPNPAM-------AAPPKPKKAANG---QAKGEDG-KDLHMFVWSSSA 370

Query: 361 SPVSDVFGN-HEFGGHHDQKEVRLAV-SPGKVEGRRENQEEYAEREDFSFGNREMMNNNN 420
           SPVSDVFGN  E+      KEVR+AV SP K +G         ER+DFSFGNR +   + 
Sbjct: 371 SPVSDVFGNGAEYNDAAAVKEVRMAVASPRKADG--------VERDDFSFGNRGVAERD- 430

Query: 421 GAEKVGDAK------------------PKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLI 480
              + GD K                  P  MPPTSVMTRLILIMVWRKLIRNPNTYSSLI
Sbjct: 431 --AEAGDEKSVAAAVSGEHGKPGLTPAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLI 490

Query: 481 GLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSM 540
           GL WSLV FRWN +MPAI+ KSISILSDAGLGMAMFSLGLFMALQPRIIACGN +A F+M
Sbjct: 491 GLIWSLVCFRWNFEMPAIILKSISILSDAGLGMAMFSLGLFMALQPRIIACGNKVATFAM 550

Query: 541 AVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILST 576
           AVRFLTGPAVMA ASIAVGLRG LL VAIVQAALPQGIVPFVFAKEY+VHPDILST
Sbjct: 551 AVRFLTGPAVMAAASIAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPDILST 570

BLAST of Sgr027415 vs. ExPASy Swiss-Prot
Match: Q5SMQ9 (Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1A PE=2 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 1.8e-225
Identity = 434/590 (73.56%), Postives = 482/590 (81.69%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTA+VPLYVAMILAYGSVKWW+IFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR
Sbjct: 11  MTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKL+VLA+L  WS++S+RG LEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG
Sbjct: 71  FIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQ-V 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLI+EQFPDTA +I SI VD D++SLDGR+  
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLITEQFPDTAANIASIVVDPDVVSLDGRRDA 190

Query: 181 LETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPT 240
           +ETE E+KEDG++HVTVR+SNASRSDI+SRRS+G SSTTPRPSNLTNAEIYSLQSSRNPT
Sbjct: 191 IETETEVKEDGRIHVTVRRSNASRSDIYSRRSMGFSSTTPRPSNLTNAEIYSLQSSRNPT 250

Query: 241 PRGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           PRGSSFNHTDFYSM+   GR+SNFG++D +G+    G TPRPSNYE+D      KP++  
Sbjct: 251 PRGSSFNHTDFYSMV---GRSSNFGAADAFGVRT--GATPRPSNYEDD----ASKPKYPL 310

Query: 301 NAT-AGGNATHYPAPNPGMFS-PTGSKNAPSNTKKPANGVVAQQKPEDGSRDLHMFVWSS 360
            A+ A   A HYPAPNP + S P G+K A +N          Q K E    DLHMFVWSS
Sbjct: 311 PASNAAPMAGHYPAPNPAVSSAPKGAKKAATN---------GQAKGE----DLHMFVWSS 370

Query: 361 SASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMMNNN- 420
           SASPVSDVFG    GG  D  +     SP K++G ++ +E+Y ER+DFSFGNR +M+ + 
Sbjct: 371 SASPVSDVFG----GGAPDYNDAAAVKSPRKMDGAKD-REDYVERDDFSFGNRGVMDRDA 430

Query: 421 -NGAEKVGD----------AKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL 480
             G EK             A P  MPPTSVMTRLILIMVWRKLIRNPNTYSSLIGL WSL
Sbjct: 431 EAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLIWSL 490

Query: 481 VSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLT 540
           V FRWN +MPAIV KSISILSDAGLGMAMFSLGLFMALQP IIACGN +A ++MAVRFL 
Sbjct: 491 VCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIACGNKVATYAMAVRFLA 550

Query: 541 GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILST 576
           GPAVMA AS AVGLRG LL VAIVQAALPQGIVPFVFAKEY+VHP ILST
Sbjct: 551 GPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPSILST 573

BLAST of Sgr027415 vs. ExPASy Swiss-Prot
Match: Q9S7Z8 (Auxin efflux carrier component 3 OS=Arabidopsis thaliana OX=3702 GN=PIN3 PE=1 SV=1)

HSP 1 Score: 691.4 bits (1783), Expect = 9.3e-198
Identity = 401/622 (64.47%), Postives = 470/622 (75.56%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           +TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFISTNNPY MNLR
Sbjct: 11  LTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQK+I+L++L +W+N ++ G LEW+IT+FSLSTLPNTLVMGIPLL  MYGE+SG
Sbjct: 71  FIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPLLIAMYGEYSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTL+LF+FE+RGA+MLI EQFP+TA SIVS  V+SD++SLDG   L
Sbjct: 131 SLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFPETAASIVSFKVESDVVSLDGHDFL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ET+AEI +DGKLHVTVRKSNA      SRRS    + TPRPSNLT AEIYSL +    TP
Sbjct: 191 ETDAEIGDDGKLHVTVRKSNA------SRRSFCGPNMTPRPSNLTGAEIYSLST----TP 250

Query: 241 RGSSFNHTDFYSMMA-AGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           RGS+FNH+DFY+MM   GGR SNFG +D+Y + +SRGPTPRPSN+EE+  A    PRF Y
Sbjct: 251 RGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEEN-CAMASSPRFGY 310

Query: 301 NATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQK--PEDG------SRDLH 360
               GG A  YPAPNP   S T S    S  K P +    QQ   P  G      +++LH
Sbjct: 311 --YPGGGAGSYPAPNPEFSSTTTSTANKSVNKNPKDVNTNQQTTLPTGGKSNSHDAKELH 370

Query: 361 MFVWSSSASPVSD-----VFG---NHEFGGHHDQ--KEVRLAV---------------SP 420
           MFVWSS+ SPVSD     VFG   +++ GG  DQ  KE+R+ V               + 
Sbjct: 371 MFVWSSNGSPVSDRAGLNVFGGAPDNDQGGRSDQGAKEIRMLVPDQSHNGETKAVAHPAS 430

Query: 421 GKVEGRRE----NQEEYAER-EDFSFGNREMMNNNNGA--EKVG-----DAKPKTMPPTS 480
           G   G ++     +EE AER +D   G  ++  N+  A   K G      ++ K MPP S
Sbjct: 431 GDFGGEQQFSFAGKEEEAERPKDAENGLNKLAPNSTAALQSKTGLGGAEASQRKNMPPAS 490

Query: 481 VMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAM 540
           VMTRLILIMVWRKLIRNPNTYSSLIGL W+LV+FRW+V MP I+ +SISILSDAGLGMAM
Sbjct: 491 VMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWHVAMPKIIQQSISILSDAGLGMAM 550

Query: 541 FSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALP 577
           FSLGLFMALQP++IACGN++A F+MAVRFLTGPAVMAVA+IA+GLRG LLRVAIVQAALP
Sbjct: 551 FSLGLFMALQPKLIACGNSVATFAMAVRFLTGPAVMAVAAIAIGLRGDLLRVAIVQAALP 610

BLAST of Sgr027415 vs. ExPASy Swiss-Prot
Match: Q8RWZ6 (Auxin efflux carrier component 4 OS=Arabidopsis thaliana OX=3702 GN=PIN4 PE=1 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 2.2e-194
Identity = 384/606 (63.37%), Postives = 445/606 (73.43%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           +TAVVPLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFISTN+PY MN R
Sbjct: 11  LTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFISTNDPYAMNFR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           F+AADTLQK+I+L +LA+W+N++K G LEW IT+FSLSTLPNTLVMGIPLL  MYG ++G
Sbjct: 71  FVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPLLIAMYGTYAG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQ+VVLQCIIWYTL+LF+FEYRGA++LI EQFP+T  SIVS  V+SD++SLDG   L
Sbjct: 131 SLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESDVVSLDGHDFL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ET+AEI  DGKLHVTVRKSNASR  +           TPRPSNLT AEIYSL S    TP
Sbjct: 191 ETDAEIGNDGKLHVTVRKSNASRRSLM---------MTPRPSNLTGAEIYSLSS----TP 250

Query: 241 RGSSFNHTDFYSMMA-AGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           RGS+FNH+DFYS+M   GGR SNFG +D+Y + +SRGPTPRPSN+EE+   N  K  F+ 
Sbjct: 251 RGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEEN---NAVKYGFYN 310

Query: 301 NATAGGNAT-HYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRD---LHMFVW 360
           N  +   A   YPAPNP   + TG    P+   K  N    Q+K    S D   LHMFVW
Sbjct: 311 NTNSSVPAAGSYPAPNPEFSTGTGVSTKPNKIPK-ENQQQLQEKDSKASHDAKELHMFVW 370

Query: 361 SSSASPVSDVFGNHEFGGHHDQ----------KEVRLAVSPGKVEGRRENQEEYAEREDF 420
           SSSASPVSDVFG    GG  D           KE+R+ VS    +       +     D 
Sbjct: 371 SSSASPVSDVFG----GGAGDNVATEQSEQGAKEIRMVVSDQPRKSNARGGGDDIGGLDS 430

Query: 421 SFGNREMMNNNNGAEKVGDAKPKT---------------MPPTSVMTRLILIMVWRKLIR 480
             G RE+     G  K+G                     MPPTSVMTRLILIMVWRKLIR
Sbjct: 431 GEGEREIEKATAGLNKMGSNSTAELEAAGGDGGGNNGTHMPPTSVMTRLILIMVWRKLIR 490

Query: 481 NPNTYSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIAC 540
           NPNTYSSLIGL W+LV++RW+V MP I+ +SISILSDAGLGMAMFSLGLFMALQP+IIAC
Sbjct: 491 NPNTYSSLIGLIWALVAYRWHVAMPKILQQSISILSDAGLGMAMFSLGLFMALQPKIIAC 550

Query: 541 GNTIAAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHP 577
           GN++A F+MAVRF+TGPA+MAVA IA+GL G LLR+AIVQAALPQGIVPFVFAKEYNVHP
Sbjct: 551 GNSVATFAMAVRFITGPAIMAVAGIAIGLHGDLLRIAIVQAALPQGIVPFVFAKEYNVHP 595

BLAST of Sgr027415 vs. ExPASy TrEMBL
Match: A0A6J1EAE3 (Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111431338 PE=3 SV=1)

HSP 1 Score: 1061.2 bits (2743), Expect = 1.7e-306
Identity = 555/591 (93.91%), Postives = 564/591 (95.43%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGA------NGGK 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG       NGGK
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGGGGGNNGGK 310

Query: 301 PRFHYNATAGG------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSR 360
           PRFHYNAT GG      NA HYPAPNPGMFSPTGSKNA  N KKPANG VAQ K EDGSR
Sbjct: 311 PRFHYNATTGGTVNANANANHYPAPNPGMFSPTGSKNAQPNAKKPANGAVAQPKTEDGSR 370

Query: 361 DLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFG 420
           DLHMFVWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAEREDFSFG
Sbjct: 371 DLHMFVWSSSASPVSDVFGNHEFGAHNDQKDVRLAVSPGKVEGRRENQEEYAEREDFSFG 430

Query: 421 NREMMNNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL 480
           NRE+MN+NN   GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL
Sbjct: 431 NREIMNSNNNNGGAEKVGDVKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSL 490

Query: 481 VSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLT 540
           VSFRWNV+MPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMAVRFLT
Sbjct: 491 VSFRWNVEMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMAVRFLT 550

Query: 541 GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 GPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 601

BLAST of Sgr027415 vs. ExPASy TrEMBL
Match: A0A6J1KLS5 (Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111495343 PE=3 SV=1)

HSP 1 Score: 1058.5 bits (2736), Expect = 1.1e-305
Identity = 555/596 (93.12%), Postives = 564/596 (94.63%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPY MNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGA---NGGKPRF 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG    NGGKPRF
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGGGNNGGKPRF 310

Query: 301 HYNATAGG--------------NATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKP 360
           HYNAT GG              NA HYPAPNPGMFSPTGSKNA  N KKPANG VAQ K 
Sbjct: 311 HYNATTGGTVNANANANANANANANHYPAPNPGMFSPTGSKNAQPNAKKPANGAVAQPKT 370

Query: 361 EDGSRDLHMFVWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAERE 420
           EDGSRDLHMFVWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAERE
Sbjct: 371 EDGSRDLHMFVWSSSASPVSDVFGNHEFGTHNDQKDVRLAVSPGKVEGRRENQEEYAERE 430

Query: 421 DFSFGNREMMNNNN---GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIG 480
           DFSFGNRE+MN+NN   GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIG
Sbjct: 431 DFSFGNREIMNSNNNNGGAEKVGDVKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIG 490

Query: 481 LTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMA 540
           LTWSLVSFRWNV+MPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMA
Sbjct: 491 LTWSLVSFRWNVEMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMA 550

Query: 541 VRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           VRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 VRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 606

BLAST of Sgr027415 vs. ExPASy TrEMBL
Match: A0A5D3BIC6 (Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002370 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 7.0e-305
Identity = 554/590 (93.90%), Postives = 563/590 (95.42%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR
Sbjct: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 120

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHYN 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG NGGKPRFHYN
Sbjct: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGNGGKPRFHYN 300

Query: 301 ATAGGNA------THYPAPNPGMFSPTGSKNA-PSNTKKPANGVVAQQKPEDGSRDLHMF 360
           AT GGNA       HYPAPNPGMFSPTGSKNA P+N KKPANG     K EDGSRDLHMF
Sbjct: 301 ATTGGNANANANVNHYPAPNPGMFSPTGSKNAQPNNAKKPANG-----KTEDGSRDLHMF 360

Query: 361 VWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM 420
           VWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM
Sbjct: 361 VWSSSASPVSDVFGNHEFGAHNDQKDVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM 420

Query: 421 NNNN-------GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLV 480
           N+NN       G EKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLV
Sbjct: 421 NSNNNGGVGVGGTEKVGDIKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLV 480

Query: 481 SFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTG 540
           SFRWNV+MPAI+AKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMAVRFLTG
Sbjct: 481 SFRWNVEMPAIIAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMAVRFLTG 540

Query: 541 PAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           PAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 541 PAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 585

BLAST of Sgr027415 vs. ExPASy TrEMBL
Match: A0A1S3CLU6 (Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103502271 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 7.0e-305
Identity = 554/590 (93.90%), Postives = 563/590 (95.42%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYG+FSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHYN 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEE+GG NGGKPRFHYN
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEEGGGNGGKPRFHYN 310

Query: 301 ATAGGNA------THYPAPNPGMFSPTGSKNA-PSNTKKPANGVVAQQKPEDGSRDLHMF 360
           AT GGNA       HYPAPNPGMFSPTGSKNA P+N KKPANG     K EDGSRDLHMF
Sbjct: 311 ATTGGNANANANVNHYPAPNPGMFSPTGSKNAQPNNAKKPANG-----KTEDGSRDLHMF 370

Query: 361 VWSSSASPVSDVFGNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM 420
           VWSSSASPVSDVFGNHEFG H+DQK+VRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM
Sbjct: 371 VWSSSASPVSDVFGNHEFGAHNDQKDVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMM 430

Query: 421 NNNN-------GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLV 480
           N+NN       G EKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLV
Sbjct: 431 NSNNNGGVGVGGTEKVGDIKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLV 490

Query: 481 SFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTG 540
           SFRWNV+MPAI+AKSISILSDAGLGMAMFSLGLFMALQPRIIACGN+IAAFSMAVRFLTG
Sbjct: 491 SFRWNVEMPAIIAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIAAFSMAVRFLTG 550

Query: 541 PAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           PAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 PAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 595

BLAST of Sgr027415 vs. ExPASy TrEMBL
Match: Q71T12 (Auxin efflux carrier component OS=Momordica charantia OX=3673 GN=AEC2 PE=2 SV=1)

HSP 1 Score: 1054.3 bits (2725), Expect = 2.0e-304
Identity = 553/579 (95.51%), Postives = 561/579 (96.89%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR
Sbjct: 11  MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLIVLAVLAVWSN+SKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG
Sbjct: 71  FIAADTLQKLIVLAVLAVWSNISKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL
Sbjct: 131 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHYN 300
           RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDG   GGKPRFHYN
Sbjct: 251 RGSSFNHTDFYSMMAAGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDG---GGKPRFHYN 310

Query: 301 ATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANG-VVAQQKPEDGSRDLHMFVWSSSA 360
           A AGGNA HYP PNPGMFSPTGSKNA  N KK ANG  V  QK E+G+RDLHMFVWSSSA
Sbjct: 311 AAAGGNAGHYPVPNPGMFSPTGSKNAQPNAKKSANGNGVVAQKTEEGNRDLHMFVWSSSA 370

Query: 361 SPVSDVF-GNHEFGGHHDQKEVRLAVSPGKVEGRRENQEEYAEREDFSFGNREMMNNNN- 420
           SPVSDVF GNHEFGG+HDQK+VRLAVSPGKVEGRRENQEEYAEREDFSFGNRE++NNNN 
Sbjct: 371 SPVSDVFGGNHEFGGNHDQKDVRLAVSPGKVEGRRENQEEYAEREDFSFGNREILNNNNG 430

Query: 421 GAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNVDMPAI 480
           GAEKVGD KPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNV+MPAI
Sbjct: 431 GAEKVGDIKPKTMPPTSVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNVEMPAI 490

Query: 481 VAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVMAVASIAV 540
           VAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVMAVASIAV
Sbjct: 491 VAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVMAVASIAV 550

Query: 541 GLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           GLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG
Sbjct: 551 GLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 586

BLAST of Sgr027415 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 806.2 bits (2081), Expect = 1.8e-233
Identity = 444/604 (73.51%), Postives = 491/604 (81.29%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           MTA+VPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFI+ NNPY MNLR
Sbjct: 11  MTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIAANNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           F+AAD+LQK+IVL++L +W  +S+ G L+WTITLFSLSTLPNTLVMGIPLLKGMYG FSG
Sbjct: 71  FLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPLLKGMYGNFSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
            LMVQIVVLQCIIWYTLMLF+FEYRGA++LISEQFPDTAGSIVSIHVDSDIMSLDGRQ L
Sbjct: 131 DLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSDIMSLDGRQPL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ETEAEIKEDGKLHVTVR+SNASRSDI+SRRS GLS+ TPRPSNLTNAEIYSLQSSRNPTP
Sbjct: 191 ETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSA-TPRPSNLTNAEIYSLQSSRNPTP 250

Query: 241 RGSSFNHTDFYSMMAA-GGRNSNFGSSD-VYGLSASRGPTPRPSNYEEDG--------GA 300
           RGSSFNHTDFYSMMA+ GGRNSNFG  + V+G   S+GPTPRPSNYEEDG        G 
Sbjct: 251 RGSSFNHTDFYSMMASGGGRNSNFGPGEAVFG---SKGPTPRPSNYEEDGGPAKPTAAGT 310

Query: 301 NGGKPRFHYNA--TAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGS- 360
             G  RFHY +  + GG   HYPAPNPGMFSP  +      T    N  V   K +DG+ 
Sbjct: 311 AAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSP--NTGGGGGTAAKGNAPVVGGKRQDGNG 370

Query: 361 RDLHMFVWSSSASPVSDVFGNHEFGGHH---------DQKEVRLAVSPGKVEGRRENQEE 420
           RDLHMFVWSSSASPVSDVFG    G HH          QK+V+++V  G       N  +
Sbjct: 371 RDLHMFVWSSSASPVSDVFGGGG-GNHHADYSTATNDHQKDVKISVPQG-----NSNDNQ 430

Query: 421 YAEREDFSFGNRE-------MMNNNNGAEKVGDAKPKTMPPTSVMTRLILIMVWRKLIRN 480
           Y ERE+FSFGN++           NN + K   A  K MPPTSVMTRLILIMVWRKLIRN
Sbjct: 431 YVEREEFSFGNKDDDSKVLATDGGNNISNKTTQA--KVMPPTSVMTRLILIMVWRKLIRN 490

Query: 481 PNTYSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACG 540
           PN+YSSL G+TWSL+SF+WN++MPA++AKSISILSDAGLGMAMFSLGLFMAL PRIIACG
Sbjct: 491 PNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFSLGLFMALNPRIIACG 550

Query: 541 NTIAAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPD 576
           N  AAF+ A+RF+ GPAVM VAS AVGLRGVLL VAI+QAALPQGIVPFVFAKEYNVHPD
Sbjct: 551 NRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQAALPQGIVPFVFAKEYNVHPD 600

BLAST of Sgr027415 vs. TAIR 10
Match: AT1G70940.1 (Auxin efflux carrier family protein )

HSP 1 Score: 691.4 bits (1783), Expect = 6.6e-199
Identity = 401/622 (64.47%), Postives = 470/622 (75.56%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           +TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFISTNNPY MNLR
Sbjct: 11  LTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFISTNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQK+I+L++L +W+N ++ G LEW+IT+FSLSTLPNTLVMGIPLL  MYGE+SG
Sbjct: 71  FIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPLLIAMYGEYSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTL+LF+FE+RGA+MLI EQFP+TA SIVS  V+SD++SLDG   L
Sbjct: 131 SLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFPETAASIVSFKVESDVVSLDGHDFL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ET+AEI +DGKLHVTVRKSNA      SRRS    + TPRPSNLT AEIYSL +    TP
Sbjct: 191 ETDAEIGDDGKLHVTVRKSNA------SRRSFCGPNMTPRPSNLTGAEIYSLST----TP 250

Query: 241 RGSSFNHTDFYSMMA-AGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           RGS+FNH+DFY+MM   GGR SNFG +D+Y + +SRGPTPRPSN+EE+  A    PRF Y
Sbjct: 251 RGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEEN-CAMASSPRFGY 310

Query: 301 NATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQK--PEDG------SRDLH 360
               GG A  YPAPNP   S T S    S  K P +    QQ   P  G      +++LH
Sbjct: 311 --YPGGGAGSYPAPNPEFSSTTTSTANKSVNKNPKDVNTNQQTTLPTGGKSNSHDAKELH 370

Query: 361 MFVWSSSASPVSD-----VFG---NHEFGGHHDQ--KEVRLAV---------------SP 420
           MFVWSS+ SPVSD     VFG   +++ GG  DQ  KE+R+ V               + 
Sbjct: 371 MFVWSSNGSPVSDRAGLNVFGGAPDNDQGGRSDQGAKEIRMLVPDQSHNGETKAVAHPAS 430

Query: 421 GKVEGRRE----NQEEYAER-EDFSFGNREMMNNNNGA--EKVG-----DAKPKTMPPTS 480
           G   G ++     +EE AER +D   G  ++  N+  A   K G      ++ K MPP S
Sbjct: 431 GDFGGEQQFSFAGKEEEAERPKDAENGLNKLAPNSTAALQSKTGLGGAEASQRKNMPPAS 490

Query: 481 VMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAM 540
           VMTRLILIMVWRKLIRNPNTYSSLIGL W+LV+FRW+V MP I+ +SISILSDAGLGMAM
Sbjct: 491 VMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWHVAMPKIIQQSISILSDAGLGMAM 550

Query: 541 FSLGLFMALQPRIIACGNTIAAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALP 577
           FSLGLFMALQP++IACGN++A F+MAVRFLTGPAVMAVA+IA+GLRG LLRVAIVQAALP
Sbjct: 551 FSLGLFMALQPKLIACGNSVATFAMAVRFLTGPAVMAVAAIAIGLRGDLLRVAIVQAALP 610

BLAST of Sgr027415 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 681.8 bits (1758), Expect = 5.3e-196
Identity = 385/598 (64.38%), Postives = 454/598 (75.92%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           +TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFIS+NNPY MNLR
Sbjct: 11  LTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFISSNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLI+L +L +W+N ++ G LEW+IT+FSLSTLPNTLVMGIPLL  MYGE+SG
Sbjct: 71  FIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPLLIAMYGEYSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTL+LF+FEYRGA++LI EQFP+T  SIVS  V+SD++SLDG   L
Sbjct: 131 SLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESDVVSLDGHDFL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ET+A+I +DGKLHVTVRKSNASR   +     G ++ TPRPSNLT AEIYSL    N TP
Sbjct: 191 ETDAQIGDDGKLHVTVRKSNASRRSFYGG---GGTNMTPRPSNLTGAEIYSL----NTTP 250

Query: 241 RGSSFNHTDFYSMMA-AGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           RGS+FNH+DFYSMM   GGR SNFG +D+Y + +SRGPTPRPSN+EE   A    PRF Y
Sbjct: 251 RGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE-SCAMASSPRFGY 310

Query: 301 NATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRDLHMFVWSSSA 360
               GG    YPAPNP     TG+K   S   K  +  V +    D +++LHMFVW S+ 
Sbjct: 311 --YPGGAPGSYPAPNPEF--STGNKTG-SKAPKENHHHVGKSNSND-AKELHMFVWGSNG 370

Query: 361 SPVSDVFGNHEFGGHHDQ---------KEVRLAVS-----PGKVEGRRENQEEYAEREDF 420
           SPVSD  G     G ++Q         KE+R+ +S      G + G    +EE    ++ 
Sbjct: 371 SPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNAGPMNGDYGGEEESERVKEV 430

Query: 421 SFGNREMMNNNNG------AEKVGDAKP-KTMPPTSVMTRLILIMVWRKLIRNPNTYSSL 480
             G  ++  N+        A + G+  P K MPP SVMTRLILIMVWRKLIRNPNTYSSL
Sbjct: 431 PNGLHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTRLILIMVWRKLIRNPNTYSSL 490

Query: 481 IGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTIAAFS 540
           IGL W+LV+FRW+V MP I+ +SISILSDAGLGMAMFSLGLFMALQP++IACGN+ A F+
Sbjct: 491 IGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNSTATFA 550

Query: 541 MAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILSTG 577
           MAVRF TGPAVMAVA++A+GLRG LLRVAIVQAALPQGIVPFVFAKEYNVHP ILSTG
Sbjct: 551 MAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQGIVPFVFAKEYNVHPAILSTG 594

BLAST of Sgr027415 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 681.0 bits (1756), Expect = 9.0e-196
Identity = 386/602 (64.12%), Postives = 450/602 (74.75%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           +TAVVPLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFISTN+PY MN R
Sbjct: 11  LTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFISTNDPYAMNFR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           F+AADTLQK+I+L +LA+W+N++K G LEW IT+FSLSTLPNTLVMGIPLL  MYG ++G
Sbjct: 71  FVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPLLIAMYGTYAG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQ+VVLQCIIWYTL+LF+FEYRGA++LI EQFP+T  SIVS  V+SD++SLDG   L
Sbjct: 131 SLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESDVVSLDGHDFL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ET+AEI  DGKLHVTVRKSNASR  +           TPRPSNLT AEIYSL S    TP
Sbjct: 191 ETDAEIGNDGKLHVTVRKSNASRRSLM---------MTPRPSNLTGAEIYSLSS----TP 250

Query: 241 RGSSFNHTDFYSMMA-AGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           RGS+FNH+DFYS+M   GGR SNFG +D+Y + +SRGPTPRPSN+EE+   N  K  F+ 
Sbjct: 251 RGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEEN---NAVKYGFYN 310

Query: 301 NATAGGNAT-HYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRD---LHMFVW 360
           N  +   A   YPAPNP   + TG    P+   K  N    Q+K    S D   LHMFVW
Sbjct: 311 NTNSSVPAAGSYPAPNPEFSTGTGVSTKPNKIPK-ENQQQLQEKDSKASHDAKELHMFVW 370

Query: 361 SSSASPVSDVFGNHEFGGHHDQ----------KEVRLAVSP------GKVEGRRENQEEY 420
           SSSASPVSDVFG    GG  D           KE+R+ VS       G   G  ++ E  
Sbjct: 371 SSSASPVSDVFG----GGAGDNVATEQSEQGAKEIRMVVSDQPRKSGGDDIGGLDSGEGE 430

Query: 421 AEREDFSFGNREMMNNNNGAEKV-----GDAKPKTMPPTSVMTRLILIMVWRKLIRNPNT 480
            E E  + G  +M +N+    +      G      MPPTSVMTRLILIMVWRKLIRNPNT
Sbjct: 431 REIEKATAGLNKMGSNSTAELEAAGGDGGGNNGTHMPPTSVMTRLILIMVWRKLIRNPNT 490

Query: 481 YSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTI 540
           YSSLIGL W+LV++RW+V MP I+ +SISILSDAGLGMAMFSLGLFMALQP+IIACGN++
Sbjct: 491 YSSLIGLIWALVAYRWHVAMPKILQQSISILSDAGLGMAMFSLGLFMALQPKIIACGNSV 550

Query: 541 AAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILS 577
           A F+MAVRF+TGPA+MAVA IA+GL G LLR+AIVQAALPQGIVPFVFAKEYNVHP ILS
Sbjct: 551 ATFAMAVRFITGPAIMAVAGIAIGLHGDLLRIAIVQAALPQGIVPFVFAKEYNVHPTILS 591

BLAST of Sgr027415 vs. TAIR 10
Match: AT1G23080.1 (Auxin efflux carrier family protein )

HSP 1 Score: 680.2 bits (1754), Expect = 1.5e-195
Identity = 385/602 (63.95%), Postives = 454/602 (75.42%), Query Frame = 0

Query: 1   MTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFISTNNPYTMNLR 60
           +TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFIS+NNPY MNLR
Sbjct: 11  LTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFISSNNPYAMNLR 70

Query: 61  FIAADTLQKLIVLAVLAVWSNVSKRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGEFSG 120
           FIAADTLQKLI+L +L +W+N ++ G LEW+IT+FSLSTLPNTLVMGIPLL  MYGE+SG
Sbjct: 71  FIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPLLIAMYGEYSG 130

Query: 121 SLMVQIVVLQCIIWYTLMLFMFEYRGARMLISEQFPDTAGSIVSIHVDSDIMSLDGRQVL 180
           SLMVQIVVLQCIIWYTL+LF+FEYRGA++LI EQFP+T  SIVS  V+SD++SLDG   L
Sbjct: 131 SLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESDVVSLDGHDFL 190

Query: 181 ETEAEIKEDGKLHVTVRKSNASRSDIFSRRSVGLSSTTPRPSNLTNAEIYSLQSSRNPTP 240
           ET+A+I +DGKLHVTVRKSNASR   +     G ++ TPRPSNLT AEIYSL    N TP
Sbjct: 191 ETDAQIGDDGKLHVTVRKSNASRRSFYGG---GGTNMTPRPSNLTGAEIYSL----NTTP 250

Query: 241 RGSSFNHTDFYSMMA-AGGRNSNFGSSDVYGLSASRGPTPRPSNYEEDGGANGGKPRFHY 300
           RGS+FNH+DFYSMM   GGR SNFG +D+Y + +SRGPTPRPSN+EE   A    PRF Y
Sbjct: 251 RGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE-SCAMASSPRFGY 310

Query: 301 NATAGGNATHYPAPNPGMFSPTGSKNAPSNTKKPANGVVAQQKPEDGSRDLHMFVWSSSA 360
               GG    YPAPNP     TG+K   S   K  +  V +    D +++LHMFVW S+ 
Sbjct: 311 --YPGGAPGSYPAPNPEF--STGNKTG-SKAPKENHHHVGKSNSND-AKELHMFVWGSNG 370

Query: 361 SPVSDVFGNHEFGGHHDQ---------KEVRLAVS---------PGKVEGRRENQEEYAE 420
           SPVSD  G     G ++Q         KE+R+ +S          G + G    +EE   
Sbjct: 371 SPVSDRAGLQVDNGANEQVGKSDQGGAKEIRMLISDHTQNGENKAGPMNGDYGGEEESER 430

Query: 421 REDFSFGNREMMNNNNG------AEKVGDAKP-KTMPPTSVMTRLILIMVWRKLIRNPNT 480
            ++   G  ++  N+        A + G+  P K MPP SVMTRLILIMVWRKLIRNPNT
Sbjct: 431 VKEVPNGLHKLRCNSTAELNPKEAIETGETVPVKHMPPASVMTRLILIMVWRKLIRNPNT 490

Query: 481 YSSLIGLTWSLVSFRWNVDMPAIVAKSISILSDAGLGMAMFSLGLFMALQPRIIACGNTI 540
           YSSLIGL W+LV+FRW+V MP I+ +SISILSDAGLGMAMFSLGLFMALQP++IACGN+ 
Sbjct: 491 YSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMALQPKLIACGNST 550

Query: 541 AAFSMAVRFLTGPAVMAVASIAVGLRGVLLRVAIVQAALPQGIVPFVFAKEYNVHPDILS 577
           A F+MAVRF TGPAVMAVA++A+GLRG LLRVAIVQAALPQGIVPFVFAKEYNVHP ILS
Sbjct: 551 ATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQGIVPFVFAKEYNVHPAILS 598

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038896073.11.2e-30694.88probable auxin efflux carrier component 1c isoform X2 [Benincasa hispida][more]
KAG6584319.11.5e-30693.76Auxin efflux carrier component 1a, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022923713.13.4e-30693.91probable auxin efflux carrier component 1c [Cucurbita moschata][more]
XP_023519167.11.0e-30593.28probable auxin efflux carrier component 1c [Cucurbita pepo subsp. pepo][more]
XP_023001099.12.2e-30593.12probable auxin efflux carrier component 1c [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9C6B82.6e-23273.51Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV... [more]
Q67UL38.7e-22874.16Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39... [more]
Q5SMQ91.8e-22573.56Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PI... [more]
Q9S7Z89.3e-19864.47Auxin efflux carrier component 3 OS=Arabidopsis thaliana OX=3702 GN=PIN3 PE=1 SV... [more]
Q8RWZ62.2e-19463.37Auxin efflux carrier component 4 OS=Arabidopsis thaliana OX=3702 GN=PIN4 PE=1 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1EAE31.7e-30693.91Auxin efflux carrier component OS=Cucurbita moschata OX=3662 GN=LOC111431338 PE=... [more]
A0A6J1KLS51.1e-30593.12Auxin efflux carrier component OS=Cucurbita maxima OX=3661 GN=LOC111495343 PE=3 ... [more]
A0A5D3BIC67.0e-30593.90Auxin efflux carrier component OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CLU67.0e-30593.90Auxin efflux carrier component OS=Cucumis melo OX=3656 GN=LOC103502271 PE=3 SV=1[more]
Q71T122.0e-30495.51Auxin efflux carrier component OS=Momordica charantia OX=3673 GN=AEC2 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73590.11.8e-23373.51Auxin efflux carrier family protein [more]
AT1G70940.16.6e-19964.47Auxin efflux carrier family protein [more]
AT1G23080.35.3e-19664.38Auxin efflux carrier family protein [more]
AT2G01420.19.0e-19664.12Auxin efflux carrier family protein [more]
AT1G23080.11.5e-19563.95Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 1..576
e-value: 6.0E-188
score: 624.1
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..576
e-value: 7.3E-147
score: 486.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 601..619
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 601..629
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 267..294
NoneNo IPR availablePANTHERPTHR31752:SF48AUXIN EFFLUX CARRIER COMPONENT 1Acoord: 1..580
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..580

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr027415.1Sgr027415.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity