Sgr027380 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr027380
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	kunitz trypsin inhibitor 2
Location	tig00153054: 915859 .. 916479 (+)
RNA-Seq Expression	Sgr027380
Synteny	Sgr027380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAATTTTGCATTGCTATCTTTTCTTTTCATCGTCATTGCCTCAACTGAGGTACATTTCTGCAAAGCCGACGCTTCGCCGGACGCCGTCCTCGATATCGACGGAAAGAAGCTCCGAGCCAGCGCCAACTACTATATCCTTCCGGTTATCAGCGGAAGAGGCGGCGGACTAGCCATCGGCAATATCCGGCGGGAATACGATAGATGTCCACTCAACGTCGTCCAAGAACCATTCGAGCTGTCCGACGGTCTTCCGGCGACATTTTCGCCTGTGAACTCTAAGAAAGGCGTCGTTCGAGTTTCCACCGATTTGAACATCCAATTCGAGGCAAATACGATCTGCGCCAAATCGACGGTGTGGAAGCTAGGCAAGTACGATCAGTATATGAAACAGTATTTCGTGACGATCGGCGGAACGAAAGGAAATCCAGGCCGTGAGACGATGGACAATTGGTTCAAAATCGAGAAGTACGGCAAAGATTACAAGTTCGTGTTCTGTCCAACTGTGTGCAAATACTGCAAAGTTATTTGCAAAGATGTGGGCATCTTCGTCAATAATGGAAAGAGGGCTCTTGCTTTGAGCGATACGCCATTCCCCGTTAAGTTCAAGGAAGTTTAA

mRNA sequence

ATGAAGAATTTTGCATTGCTATCTTTTCTTTTCATCGTCATTGCCTCAACTGAGGTACATTTCTGCAAAGCCGACGCTTCGCCGGACGCCGTCCTCGATATCGACGGAAAGAAGCTCCGAGCCAGCGCCAACTACTATATCCTTCCGGTTATCAGCGGAAGAGGCGGCGGACTAGCCATCGGCAATATCCGGCGGGAATACGATAGATGTCCACTCAACGTCGTCCAAGAACCATTCGAGCTGTCCGACGGTCTTCCGGCGACATTTTCGCCTGTGAACTCTAAGAAAGGCGTCGTTCGAGTTTCCACCGATTTGAACATCCAATTCGAGGCAAATACGATCTGCGCCAAATCGACGGTGTGGAAGCTAGGCAAGTACGATCAGTATATGAAACAGTATTTCGTGACGATCGGCGGAACGAAAGGAAATCCAGGCCGTGAGACGATGGACAATTGGTTCAAAATCGAGAAGTACGGCAAAGATTACAAGTTCGTGTTCTGTCCAACTGTGTGCAAATACTGCAAAGTTATTTGCAAAGATGTGGGCATCTTCGTCAATAATGGAAAGAGGGCTCTTGCTTTGAGCGATACGCCATTCCCCGTTAAGTTCAAGGAAGTTTAA

Coding sequence (CDS)

ATGAAGAATTTTGCATTGCTATCTTTTCTTTTCATCGTCATTGCCTCAACTGAGGTACATTTCTGCAAAGCCGACGCTTCGCCGGACGCCGTCCTCGATATCGACGGAAAGAAGCTCCGAGCCAGCGCCAACTACTATATCCTTCCGGTTATCAGCGGAAGAGGCGGCGGACTAGCCATCGGCAATATCCGGCGGGAATACGATAGATGTCCACTCAACGTCGTCCAAGAACCATTCGAGCTGTCCGACGGTCTTCCGGCGACATTTTCGCCTGTGAACTCTAAGAAAGGCGTCGTTCGAGTTTCCACCGATTTGAACATCCAATTCGAGGCAAATACGATCTGCGCCAAATCGACGGTGTGGAAGCTAGGCAAGTACGATCAGTATATGAAACAGTATTTCGTGACGATCGGCGGAACGAAAGGAAATCCAGGCCGTGAGACGATGGACAATTGGTTCAAAATCGAGAAGTACGGCAAAGATTACAAGTTCGTGTTCTGTCCAACTGTGTGCAAATACTGCAAAGTTATTTGCAAAGATGTGGGCATCTTCGTCAATAATGGAAAGAGGGCTCTTGCTTTGAGCGATACGCCATTCCCCGTTAAGTTCAAGGAAGTTTAA

Protein sequence

MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAIGNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKDVGIFVNNGKRALALSDTPFPVKFKEV

Homology

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BLAST of Sgr027380 vs. NCBI nr
Match: XP_022137472.1 (kunitz trypsin inhibitor 2 [Momordica charantia])

HSP 1 Score: 344.7 bits (883), Expect = 5.4e-91
Identity = 159/206 (77.18%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALL FLF +IASTEV  CK+DASPDAVLDIDGKKLRA  NYYILPVI GRGGGL +
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           GNI   +D+CPL+VVQE FE+S+GLPATF+PVN KKGVVRVSTDLN++FEA+T+CAKSTV
Sbjct: 61  GNI--GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WKL K+D+  +Q+ V IGG +GNPG+ET+DNWFKIEK+GKDYKFVFCP+VCK+CKVICKD
Sbjct: 121 WKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VGIF+ NG RALALSDTPFPV FK+V
Sbjct: 181 VGIFIKNGNRALALSDTPFPVMFKKV 204

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BLAST of Sgr027380 vs. NCBI nr
Match: XP_022923703.1 (kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin inhibitor 2 [Cucurbita maxima] >KAG6584289.1 Kunitz trypsin inhibitor 5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019882.1 Kunitz trypsin inhibitor 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 329.3 bits (843), Expect = 2.3e-86
Identity = 157/206 (76.21%), Postives = 178/206 (86.41%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALLSFLFI++AS EV   +ADASPDAV DIDGKKLRA  NYYILPV  GRGGGLA+
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           GN+  + D+CPLNVVQE FEL +GLPA F PVN KKGVVRVSTDLN+QFEA+TICA STV
Sbjct: 61  GNL--QSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WKL K+D+  KQ+F+TIGGT+GNPG +T+DNWFKIEK+G DYKF FCPTVC +CKV+C+D
Sbjct: 121 WKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VGIF  NGKRALALSDTPFPV FKEV
Sbjct: 181 VGIFFKNGKRALALSDTPFPVMFKEV 203

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BLAST of Sgr027380 vs. NCBI nr
Match: XP_008437071.1 (PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis melo var. makuwa])

HSP 1 Score: 328.2 bits (840), Expect = 5.2e-86
Identity = 158/210 (75.24%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 1   MKNFALLSFLF--IVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGR-GGG 60
           MK FALLSFLF  IVIAS+E+ FC+ADASPDAVLD DGKKLR S  YYILP   G  GGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAK 120
           LAIGNIR+EYDRC +NVVQE +E SDG P TF P+N KKGVVRVSTDLNI+F+A T C K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGK-DYKFVFCPTVCKYCKV 180
           STVWKLG +D+Y++QYFVTIGGTKGNPGRET +NWFKIEKYGK +YK V+CP VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 ICKDVGIFVNNGKRALALSDTPFPVKFKEV 207
           +CKD+GIF NNG R L LSDTPFPV FK+V
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

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BLAST of Sgr027380 vs. NCBI nr
Match: XP_008437082.1 (PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. makuwa])

HSP 1 Score: 325.9 bits (834), Expect = 2.6e-85
Identity = 150/206 (72.82%), Postives = 176/206 (85.44%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALL FLFIVIAS+EV FC+ADASPDAVLD DGKKLRA   YYIL V S   GGL+I
Sbjct: 1   MKNFALLCFLFIVIASSEVRFCRADASPDAVLDTDGKKLRAGDQYYILSVYSRNSGGLSI 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           G I   Y++CP+N++ E ++  DGLPATFSPVN KKGVVRVSTDLNI+FEA+T C  STV
Sbjct: 61  GGI-YGYEKCPINILPESYDYLDGLPATFSPVNPKKGVVRVSTDLNIEFEASTRCGISTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WK+GK+DQY+KQYFVT+GGTKGNPGRET+ NWFK+EK+GK+YKFV+CPTVCKYCKV+CKD
Sbjct: 121 WKVGKFDQYLKQYFVTMGGTKGNPGRETIGNWFKVEKHGKNYKFVYCPTVCKYCKVMCKD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VG+F  NG+R  AL+D PFPV FK+V
Sbjct: 181 VGLFYKNGRRIFALNDAPFPVMFKKV 205

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BLAST of Sgr027380 vs. NCBI nr
Match: XP_038895396.1 (miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa hispida])

HSP 1 Score: 325.5 bits (833), Expect = 3.4e-85
Identity = 150/206 (72.82%), Postives = 174/206 (84.47%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALLSFLFIVI S+EV FC+AD SPDAVLD DGKKLR+S  YYIL V S   GGL+I
Sbjct: 1   MKNFALLSFLFIVIVSSEVRFCRADVSPDAVLDTDGKKLRSSDQYYILSVYSRNSGGLSI 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           G+I++  ++CP+N++ E +E   GL ATF P+N KKGVVRVSTDLNIQFEA+T C +STV
Sbjct: 61  GSIQQGKEKCPINILPESYEYLHGLAATFFPINPKKGVVRVSTDLNIQFEASTRCGESTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WK+GK+DQY+KQYFVTIGG KGNPGRET+ NWFKIEKYG +YK V CPTVCKYCKVICKD
Sbjct: 121 WKIGKFDQYLKQYFVTIGGVKGNPGRETVGNWFKIEKYGNNYKLVHCPTVCKYCKVICKD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VGIF  NGKR LAL+D+PFPV FK+V
Sbjct: 181 VGIFYKNGKRVLALNDSPFPVMFKKV 206

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BLAST of Sgr027380 vs. ExPASy Swiss-Prot
Match: Q9LMU2 (Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1)

HSP 1 Score: 208.8 bits (530), Expect = 6.0e-53
Identity = 99/199 (49.75%), Postives = 139/199 (69.85%), Query Frame = 0


Query: 7   LSFLFIVIASTEVH-FCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAIGNIRR 66
           L ++F+++A    H     +A+ + V DI+GK L    NYYILPVI GRGGGL + N++ 
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  EYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTVWKLGK 126
           E   CP +V+Q+ FE+S GLP  FSP + K   + VSTD+NI+F      + +++W+L  
Sbjct: 64  E--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELAN 123

Query: 127 YDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKDVGIFV 186
           +D+  KQ+F++  G +GNPG++T+DNWFKI+K+ KDYK  FCPTVC +CKVIC+DVG+FV
Sbjct: 124 FDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFV 183

Query: 187 NNGKRALALSDTPFPVKFK 205
            +GKR LALSD P  V FK
Sbjct: 184 QDGKRRLALSDVPLKVMFK 193

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BLAST of Sgr027380 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 196.4 bits (498), Expect = 3.1e-49
Identity = 107/216 (49.54%), Postives = 132/216 (61.11%), Query Frame = 0


Query: 1   MKNFALLSFLF-----IVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRG 60
           MK   +LS  F     ++ A+       AD++P+ VLDIDG+KLR   NYYI+PV+   G
Sbjct: 1   MKELTMLSLSFFFVSALLAAAANPLLSAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHG 60

Query: 61  GGLAI-GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTI 120
           GGL +          CP  VVQ   E+    P  F P N K+ VVRVSTDLNI F A   
Sbjct: 61  GGLTVSATTPNGTFVCPPRVVQTRKEVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMP 120

Query: 121 C--AKSTVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKY--GKDYKFVFCPTV 180
           C    STVW+L KYD+   QYFVTIGG KGNPG ET+ +WFKIE++     YK VFCPTV
Sbjct: 121 CRWTSSTVWRLDKYDESTGQYFVTIGGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPTV 180

Query: 181 CKYCKVICKDVGIFVN-NGKRALALSDTPFPVKFKE 206
           C  CKV C DVGI+++  G+R LALSD PF  +F +
Sbjct: 181 CGSCKVKCGDVGIYIDQKGRRRLALSDKPFAFEFNK 216

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BLAST of Sgr027380 vs. ExPASy Swiss-Prot
Match: P32765 (21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.5e-38
Identity = 91/210 (43.33%), Postives = 120/210 (57.14%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGR-GGGLA 60
           MK    +  L     S    F  A+A+   VLD DG +L+    YY+L  ISG  GGGLA
Sbjct: 1   MKTATAVVLLLFAFTSKSYFFGVANAANSPVLDTDGDELQTGVQYYVLSSISGAGGGGLA 60

Query: 61  IGNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFE--ANTICAK 120
           +G  R     CP  VVQ   +L +G P  FS  +SK  VVRVSTD+NI+F    + +C+ 
Sbjct: 61  LG--RATGQSCPEIVVQRRSDLDNGTPVIFSNADSKDDVVRVSTDVNIEFVPIRDRLCST 120

Query: 121 STVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYG-KDYKFVFCPTVCKYCKV 180
           STVW+L  YD    +++VT  G KG PG  T+ +WFKIEK G   YKF FCP+VC  C  
Sbjct: 121 STVWRLDNYDNSAGKWWVTTDGVKGEPGPNTLCSWFKIEKAGVLGYKFRFCPSVCDSCTT 180

Query: 181 ICKDVGIFV-NNGKRALALSDTPFPVKFKE 206
           +C D+G    ++G+  LALSD  +   FK+
Sbjct: 181 LCSDIGRHSDDDGQIRLALSDNEWAWMFKK 208

[Close]

BLAST of Sgr027380 vs. ExPASy Swiss-Prot
Match: Q8RXD5 (Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.3e-36
Identity = 88/198 (44.44%), Postives = 120/198 (60.61%), Query Frame = 0


Query: 11  FIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAIGNIRREYDRC 70
           ++V+A T V    A  +  AV+DIDG  +    +YY+LPVI GRGGGL +    R    C
Sbjct: 13  YLVLALTAV---LASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG--RGGQPC 72

Query: 71  PLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEAN-TICAKSTVWKLGKYDQY 130
           P ++VQE  E+ +G+P  FS    K   V  S +LNI+ +   TIC +ST W++G++D  
Sbjct: 73  PYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHE 132

Query: 131 MKQYFVTIGGTKGNPGRETMDNWFKIEKYGKD-YKFVFCPTVCKYCKVICKDVGIFVNN- 190
            KQYFV  G      G++++ ++FKIEK G+D YKFVFCP  C      C DVGIF++  
Sbjct: 133 RKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDEL 192

Query: 191 GKRALALSDTPFPVKFKE 206
           G R LALSD PF V FK+
Sbjct: 193 GVRRLALSDKPFLVMFKK 204

[Close]

BLAST of Sgr027380 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 2.5e-22
Identity = 64/184 (34.78%), Postives = 90/184 (48.91%), Query Frame = 0


Query: 26  ASPDAVLDIDGKKLRASANYYILPVISGRGGGLAIGNIRREYDRCPLNVVQEPFELSDGL 85
           A+P  V D +G +L A  +YY+LP   G GGGL +         CPL V QE  E   G 
Sbjct: 22  AAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMA---PRVLPCPLLVAQETDERRKGF 81

Query: 86  PATFSP----VNSKKGVVRVSTDLNIQFEANTICAKSTVWKLGKYDQYMKQYFVTIGGTK 145
           P  F+P       +   +RVSTD+ I+F A TIC +ST W +G  D+ +      + G  
Sbjct: 82  PVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTEWHVG--DEPLTGARRVVTGPL 141

Query: 146 GNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKDVGIFVNNGKRALALSDTPFPV 205
             P     +N F++EKYG  YK V        C+  C+D+G+  +  +  L  S  P  V
Sbjct: 142 IGPSPSGRENAFRVEKYGGGYKLV-------SCRDSCQDLGVSRDGARAWLGASQPPHVV 193

[Close]

BLAST of Sgr027380 vs. ExPASy TrEMBL
Match: A0A6J1C8C4 (kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 2.6e-91
Identity = 159/206 (77.18%), Postives = 184/206 (89.32%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALL FLF +IASTEV  CK+DASPDAVLDIDGKKLRA  NYYILPVI GRGGGL +
Sbjct: 1   MKNFALLCFLFALIASTEVRICKSDASPDAVLDIDGKKLRAGVNYYILPVIRGRGGGLTL 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           GNI   +D+CPL+VVQE FE+S+GLPATF+PVN KKGVVRVSTDLN++FEA+T+CAKSTV
Sbjct: 61  GNI--GHDKCPLHVVQEQFEISNGLPATFAPVNPKKGVVRVSTDLNVEFEASTVCAKSTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WKL K+D+  +Q+ V IGG +GNPG+ET+DNWFKIEK+GKDYKFVFCP+VCK+CKVICKD
Sbjct: 121 WKLDKFDESTRQWLVAIGGIRGNPGQETLDNWFKIEKHGKDYKFVFCPSVCKFCKVICKD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VGIF+ NG RALALSDTPFPV FK+V
Sbjct: 181 VGIFIKNGNRALALSDTPFPVMFKKV 204

[Close]

BLAST of Sgr027380 vs. ExPASy TrEMBL
Match: A0A6J1KRM6 (kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 157/206 (76.21%), Postives = 178/206 (86.41%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALLSFLFI++AS EV   +ADASPDAV DIDGKKLRA  NYYILPV  GRGGGLA+
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           GN+  + D+CPLNVVQE FEL +GLPA F PVN KKGVVRVSTDLN+QFEA+TICA STV
Sbjct: 61  GNL--QSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WKL K+D+  KQ+F+TIGGT+GNPG +T+DNWFKIEK+G DYKF FCPTVC +CKV+C+D
Sbjct: 121 WKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VGIF  NGKRALALSDTPFPV FKEV
Sbjct: 181 VGIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Sgr027380 vs. ExPASy TrEMBL
Match: A0A6J1EAD6 (kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 1.1e-86
Identity = 157/206 (76.21%), Postives = 178/206 (86.41%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAI 60
           MKNFALLSFLFI++AS EV   +ADASPDAV DIDGKKLRA  NYYILPV  GRGGGLA+
Sbjct: 1   MKNFALLSFLFILLAS-EVRVSRADASPDAVRDIDGKKLRAGVNYYILPVFRGRGGGLAL 60

Query: 61  GNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTV 120
           GN+  + D+CPLNVVQE FEL +GLPA F PVN KKGVVRVSTDLN+QFEA+TICA STV
Sbjct: 61  GNL--QSDKCPLNVVQEQFELMNGLPAAFLPVNPKKGVVRVSTDLNVQFEASTICATSTV 120

Query: 121 WKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKD 180
           WKL K+D+  KQ+F+TIGGT+GNPG +T+DNWFKIEK+G DYKF FCPTVC +CKV+C+D
Sbjct: 121 WKLDKFDESTKQWFITIGGTRGNPGVKTVDNWFKIEKHGNDYKFKFCPTVCDFCKVMCRD 180

Query: 181 VGIFVNNGKRALALSDTPFPVKFKEV 207
           VGIF  NGKRALALSDTPFPV FKEV
Sbjct: 181 VGIFFKNGKRALALSDTPFPVMFKEV 203

[Close]

BLAST of Sgr027380 vs. ExPASy TrEMBL
Match: A0A5D3BJ25 (Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002780 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.5e-86
Identity = 158/210 (75.24%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 1   MKNFALLSFLF--IVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGR-GGG 60
           MK FALLSFLF  IVIAS+E+ FC+ADASPDAVLD DGKKLR S  YYILP   G  GGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAK 120
           LAIGNIR+EYDRC +NVVQE +E SDG P TF P+N KKGVVRVSTDLNI+F+A T C K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGK-DYKFVFCPTVCKYCKV 180
           STVWKLG +D+Y++QYFVTIGGTKGNPGRET +NWFKIEKYGK +YK V+CP VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 ICKDVGIFVNNGKRALALSDTPFPVKFKEV 207
           +CKD+GIF NNG R L LSDTPFPV FK+V
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of Sgr027380 vs. ExPASy TrEMBL
Match: A0A1S3AT47 (miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 2.5e-86
Identity = 158/210 (75.24%), Postives = 177/210 (84.29%), Query Frame = 0


Query: 1   MKNFALLSFLF--IVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGR-GGG 60
           MK FALLSFLF  IVIAS+E+ FC+ADASPDAVLD DGKKLR S  YYILP   G  GGG
Sbjct: 1   MKKFALLSFLFIAIVIASSELRFCRADASPDAVLDTDGKKLRVSNKYYILPAFEGSGGGG 60

Query: 61  LAIGNIRREYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAK 120
           LAIGNIR+EYDRC +NVVQE +E SDG P TF P+N KKGVVRVSTDLNI+F+A T C K
Sbjct: 61  LAIGNIRKEYDRCGINVVQERYEQSDGDPTTFLPINPKKGVVRVSTDLNIEFDATTRCRK 120

Query: 121 STVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGK-DYKFVFCPTVCKYCKV 180
           STVWKLG +D+Y++QYFVTIGGTKGNPGRET +NWFKIEKYGK +YK V+CP VCKYCKV
Sbjct: 121 STVWKLGTFDRYLRQYFVTIGGTKGNPGRETTENWFKIEKYGKGNYKLVYCPRVCKYCKV 180

Query: 181 ICKDVGIFVNNGKRALALSDTPFPVKFKEV 207
           +CKD+GIF NNG R L LSDTPFPV FK+V
Sbjct: 181 MCKDIGIFENNGIRGLVLSDTPFPVIFKKV 210

[Close]

BLAST of Sgr027380 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 208.8 bits (530), Expect = 4.3e-54
Identity = 99/199 (49.75%), Postives = 139/199 (69.85%), Query Frame = 0


Query: 7   LSFLFIVIASTEVH-FCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAIGNIRR 66
           L ++F+++A    H     +A+ + V DI+GK L    NYYILPVI GRGGGL + N++ 
Sbjct: 4   LLYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKT 63

Query: 67  EYDRCPLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANTICAKSTVWKLGK 126
           E   CP +V+Q+ FE+S GLP  FSP + K   + VSTD+NI+F      + +++W+L  
Sbjct: 64  E--TCPTSVIQDQFEVSQGLPVKFSPYD-KSRTIPVSTDVNIKF------SPTSIWELAN 123

Query: 127 YDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKVICKDVGIFV 186
           +D+  KQ+F++  G +GNPG++T+DNWFKI+K+ KDYK  FCPTVC +CKVIC+DVG+FV
Sbjct: 124 FDETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPTVCNFCKVICRDVGVFV 183

Query: 187 NNGKRALALSDTPFPVKFK 205
            +GKR LALSD P  V FK
Sbjct: 184 QDGKRRLALSDVPLKVMFK 193

[Close]

BLAST of Sgr027380 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 154.5 bits (389), Expect = 9.6e-38
Identity = 88/198 (44.44%), Postives = 120/198 (60.61%), Query Frame = 0


Query: 11  FIVIASTEVHFCKADASPDAVLDIDGKKLRASANYYILPVISGRGGGLAIGNIRREYDRC 70
           ++V+A T V    A  +  AV+DIDG  +    +YY+LPVI GRGGGL +    R    C
Sbjct: 13  YLVLALTAV---LASNAYGAVVDIDGNAM-FHESYYVLPVIRGRGGGLTLAG--RGGQPC 72

Query: 71  PLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEAN-TICAKSTVWKLGKYDQY 130
           P ++VQE  E+ +G+P  FS    K   V  S +LNI+ +   TIC +ST W++G++D  
Sbjct: 73  PYDIVQESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHE 132

Query: 131 MKQYFVTIGGTKGNPGRETMDNWFKIEKYGKD-YKFVFCPTVCKYCKVICKDVGIFVNN- 190
            KQYFV  G      G++++ ++FKIEK G+D YKFVFCP  C      C DVGIF++  
Sbjct: 133 RKQYFVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCDSGNPKCSDVGIFIDEL 192

Query: 191 GKRALALSDTPFPVKFKE 206
           G R LALSD PF V FK+
Sbjct: 193 GVRRLALSDKPFLVMFKK 204

[Close]

BLAST of Sgr027380 vs. TAIR 10
Match: AT1G73325.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 110.2 bits (274), Expect = 2.1e-24
Identity = 73/211 (34.60%), Postives = 115/211 (54.50%), Query Frame = 0


Query: 1   MKNFALLSFLFIVIASTEVHFCKADASP-DAVLDIDGKKLRASANYYILPVISGRGGGLA 60
           M+   L      V+++       ADA+P   VLDI G  ++++  YYI+P   G GGGL 
Sbjct: 1   MEKLTLSFITLTVLSAIFTAASAADATPSQVVLDIAGHPVQSNVQYYIIPAKIGTGGGLI 60

Query: 61  IGNIR-REYDRC-PLNVVQEPFELSDGLPATFSPVNSKKGVVRVSTDLNIQFEANT-ICA 120
             N      D C  L++VQ       GLP TFSP+N+K   V++S  LN++F++   +C 
Sbjct: 61  PSNRNLSTQDLCLNLDIVQSSSPFVSGLPVTFSPLNTKVKHVQLSASLNLEFDSTVWLCP 120

Query: 121 KSTVWKLGKYDQYMKQYFVTIGGTKGNPGRETMDNWFKIEKYGKDYKFVFCPTVCKYCKV 180
            S VW++  +   +++ FV+IGG KG       ++WF+I++ G  YK ++CP       V
Sbjct: 121 DSKVWRI-DHSVQLRKSFVSIGGQKGKG-----NSWFQIQEDGDAYKLMYCPI---SSIV 180

Query: 181 ICKDVGIFV-NNGKRALALS-DTPFPVKFKE 206
            C +V + + ++G R L LS D  F VKF++
Sbjct: 181 ACINVSLEIDDHGVRRLVLSTDQSFVVKFQK 202

The following BLAST results are available for this feature:

BLAST of Sgr027380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137472.1	5.4e-91	77.18	kunitz trypsin inhibitor 2 [Momordica charantia]	[more]
XP_022923703.1	2.3e-86	76.21	kunitz trypsin inhibitor 2 [Cucurbita moschata] >XP_023001808.1 kunitz trypsin i...	[more]
XP_008437071.1	5.2e-86	75.24	PREDICTED: miraculin-like [Cucumis melo] >TYJ99074.1 miraculin-like [Cucumis mel...	[more]
XP_008437082.1	2.6e-85	72.82	PREDICTED: miraculin [Cucumis melo] >TYJ99076.1 miraculin [Cucumis melo var. mak...	[more]
XP_038895396.1	3.4e-85	72.82	miraculin-like [Benincasa hispida] >XP_038895399.1 miraculin-like [Benincasa his...	[more]

BLAST of Sgr027380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LMU2	6.0e-53	49.75	Kunitz trypsin inhibitor 5 OS=Arabidopsis thaliana OX=3702 GN=KTI5 PE=2 SV=1	[more]
P13087	3.1e-49	49.54	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P32765	2.5e-38	43.33	21 kDa seed protein OS=Theobroma cacao OX=3641 GN=ASP PE=2 SV=1	[more]
Q8RXD5	1.3e-36	44.44	Kunitz trypsin inhibitor 4 OS=Arabidopsis thaliana OX=3702 GN=KTI4 PE=2 SV=1	[more]
P29421	2.5e-22	34.78	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Sgr027380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C8C4	2.6e-91	77.18	kunitz trypsin inhibitor 2 OS=Momordica charantia OX=3673 GN=LOC111008908 PE=3 S...	[more]
A0A6J1KRM6	1.1e-86	76.21	kunitz trypsin inhibitor 2 OS=Cucurbita maxima OX=3661 GN=LOC111495838 PE=3 SV=1	[more]
A0A6J1EAD6	1.1e-86	76.21	kunitz trypsin inhibitor 2 OS=Cucurbita moschata OX=3662 GN=LOC111431333 PE=3 SV...	[more]
A0A5D3BJ25	2.5e-86	75.24	Miraculin-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G00278...	[more]
A0A1S3AT47	2.5e-86	75.24	miraculin-like OS=Cucumis melo OX=3656 GN=LOC103482604 PE=3 SV=1	[more]

BLAST of Sgr027380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G17860.1	4.3e-54	49.75	Kunitz family trypsin and protease inhibitor protein	[more]
AT1G73260.1	9.6e-38	44.44	kunitz trypsin inhibitor 1	[more]
AT1G73325.1	2.1e-24	34.60	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	PRINTS	PR00291	KUNITZINHBTR	coord: 70..90 score: 35.48 coord: 30..59 score: 43.59 coord: 152..171 score: 25.69 coord: 175..204 score: 25.75
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 30..206 e-value: 1.5E-72 score: 257.0
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 31..204 e-value: 1.5E-58 score: 197.6
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 21..205
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 31..47
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 30..204 e-value: 2.7886E-68 score: 204.884
None	No IPR available	GENE3D	2.80.10.50		coord: 25..205 e-value: 7.1E-73 score: 246.0
None	No IPR available	PANTHER	PTHR33107:SF26	MIRACULIN-LIKE	coord: 21..205
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 27..205

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr027380.1	Sgr027380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity