Sgr027212 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr027212
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionE3 ubiquitin-protein ligase ATL42-like
Locationtig00153048: 2074335 .. 2075602 (-)
RNA-Seq ExpressionSgr027212
SyntenySgr027212
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTAAGTTCAGTAGGATCATTCTGGTAATTATGACTAATTTGCTACTTCATGTCAATGCACAAGGGCCGCCTCCACCAAAAGCTGATTCTGGTATTCCACAAAACCTCACCATTGTTATTGGAATCCTCTCCATCATGCTCTCCATGGCCTTCATCCTTGTTCTTTATGCGAAATTCTGTCACCAGAATTCCCATCTTCGTGGCGAACCGCAAGCTCATCTTGGACTTGGACTTCTCAGATCAGACTCGAGATTTTCTGGGGTTGATCAGAAGGTGATTGATTCACTTCCTTTCTTTAGATTTTCATCACTTAAAGGATCGAGGGAAGGGCTGGAATGTGCAGTTTGCCTGTCCAAATTTGAAGATATCGAAGTTCTTCGGTTACTGCCGAAATGCAAGCATGCCTTTCACATTGGATGCATTGACCATTGGCTTGAAAAGCACTCGAGCTGTCCGCTCTGCCGGTGTAGGATCAGCGTCGAGGACGTTGCATACTTTAGCAATTCCAACAGTATGAGATTCATGAGGAACAACAACCAGCCAGAGCTGCCACAAGATCCAAACATGGAGCTCTTTGTGCAGAGAGAGGAAGATCATCATAACTCTTCAAGGTTCAATATTGGAAGCAGCTTCAGAAAAATTGGAAAGGATAATAAGGAAAAAGAAGTCTTAATCGAAAATGAAGCCGGTGCAAGTGCAAGTGAGGAGCAGGTGGTTTTTCACAAGCATAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAATTTGAGTTCTTCTGACCTCATGTTCTTGAACACAGAGATGCTTGGGGCCATGTCAAGCAATAGATTCTCTTCCTTGGATTCAGACAACATTCGTCGGTTGGCAACAAGAGAAACTGAGGGCAAGAAGATTACAAACATTAAGGAGGAGATGGAAATGAAGAGATCATTTGAGAGTAGGATAAGCATAGCTGAGAAAAACAGCTTAGTTTCAATGACAGGTATTCCCTCTGTATCAGATTCTAATGCAAGTACATCAAGGACAACAAATCTTAATGAAAAAGATCTGTCTCAGACACCACTGCTTTCTCAAGATTTGGTAATTTCAATGAGAATGCAATAAGAGAAACTTCTGTAATTGAAGCAGATGCAAAAGATTACAGAATTAGGCAGATTTGGTTGCCAATAGCCAGAAGAACAGTCCAATGGTTTGTCAATAGGGAACAAAGGACTCAACTGTCAAACTACACGCAGCAGCCAGATGTATAG

mRNA sequence

ATGAGTAAGTTCAGTAGGATCATTCTGGTAATTATGACTAATTTGCTACTTCATGTCAATGCACAAGGGCCGCCTCCACCAAAAGCTGATTCTGGTATTCCACAAAACCTCACCATTGTTATTGGAATCCTCTCCATCATGCTCTCCATGGCCTTCATCCTTGTTCTTTATGCGAAATTCTGTCACCAGAATTCCCATCTTCGTGGCGAACCGCAAGCTCATCTTGGACTTGGACTTCTCAGATCAGACTCGAGATTTTCTGGGGTTGATCAGAAGGTGATTGATTCACTTCCTTTCTTTAGATTTTCATCACTTAAAGGATCGAGGGAAGGGCTGGAATGTGCAGTTTGCCTGTCCAAATTTGAAGATATCGAAGTTCTTCGGTTACTGCCGAAATGCAAGCATGCCTTTCACATTGGATGCATTGACCATTGGCTTGAAAAGCACTCGAGCTGTCCGCTCTGCCGGTGTAGGATCAGCGTCGAGGACGTTGCATACTTTAGCAATTCCAACAGTATGAGATTCATGAGGAACAACAACCAGCCAGAGCTGCCACAAGATCCAAACATGGAGCTCTTTGTGCAGAGAGAGGAAGATCATCATAACTCTTCAAGGTTCAATATTGGAAGCAGCTTCAGAAAAATTGGAAAGGATAATAAGGAAAAAGAAGTCTTAATCGAAAATGAAGCCGGTGCAAGTGCAAGTGAGGAGCAGGTGGTTTTTCACAAGCATAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAATTTGAGTTCTTCTGACCTCATGTTCTTGAACACAGAGATGCTTGGGGCCATGTCAAGCAATAGATTCTCTTCCTTGGATTCAGACAACATTCGTCGGTTGGCAACAAGAGAAACTGAGGGCAAGAAGATTACAAACATTAAGGAGGAGATGGAAATGAAGAGATCATTTGAGAGTAGGATAAGCATAGCTGAGAAAAACAGCTTAGTTTCAATGACAGACACCACTGCTTTCTCAAGATTTGGTAATTTCAATGAGAATGCAATAAGAGAAACTTCTGTAATTGAAGCAGATGCAAAAGATTACAGAATTAGGCAGATTTGGTTGCCAATAGCCAGAAGAACAGTCCAATGGTTTGTCAATAGGGAACAAAGGACTCAACTGTCAAACTACACGCAGCAGCCAGATGTATAG

Coding sequence (CDS)

ATGAGTAAGTTCAGTAGGATCATTCTGGTAATTATGACTAATTTGCTACTTCATGTCAATGCACAAGGGCCGCCTCCACCAAAAGCTGATTCTGGTATTCCACAAAACCTCACCATTGTTATTGGAATCCTCTCCATCATGCTCTCCATGGCCTTCATCCTTGTTCTTTATGCGAAATTCTGTCACCAGAATTCCCATCTTCGTGGCGAACCGCAAGCTCATCTTGGACTTGGACTTCTCAGATCAGACTCGAGATTTTCTGGGGTTGATCAGAAGGTGATTGATTCACTTCCTTTCTTTAGATTTTCATCACTTAAAGGATCGAGGGAAGGGCTGGAATGTGCAGTTTGCCTGTCCAAATTTGAAGATATCGAAGTTCTTCGGTTACTGCCGAAATGCAAGCATGCCTTTCACATTGGATGCATTGACCATTGGCTTGAAAAGCACTCGAGCTGTCCGCTCTGCCGGTGTAGGATCAGCGTCGAGGACGTTGCATACTTTAGCAATTCCAACAGTATGAGATTCATGAGGAACAACAACCAGCCAGAGCTGCCACAAGATCCAAACATGGAGCTCTTTGTGCAGAGAGAGGAAGATCATCATAACTCTTCAAGGTTCAATATTGGAAGCAGCTTCAGAAAAATTGGAAAGGATAATAAGGAAAAAGAAGTCTTAATCGAAAATGAAGCCGGTGCAAGTGCAAGTGAGGAGCAGGTGGTTTTTCACAAGCATAAACACAAGATTCTTGTATCTGACTTTGTTTTCATGAACAGATGGAGCAATTTGAGTTCTTCTGACCTCATGTTCTTGAACACAGAGATGCTTGGGGCCATGTCAAGCAATAGATTCTCTTCCTTGGATTCAGACAACATTCGTCGGTTGGCAACAAGAGAAACTGAGGGCAAGAAGATTACAAACATTAAGGAGGAGATGGAAATGAAGAGATCATTTGAGAGTAGGATAAGCATAGCTGAGAAAAACAGCTTAGTTTCAATGACAGACACCACTGCTTTCTCAAGATTTGGTAATTTCAATGAGAATGCAATAAGAGAAACTTCTGTAATTGAAGCAGATGCAAAAGATTACAGAATTAGGCAGATTTGGTTGCCAATAGCCAGAAGAACAGTCCAATGGTTTGTCAATAGGGAACAAAGGACTCAACTGTCAAACTACACGCAGCAGCCAGATGTATAG

Protein sequence

MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNNQPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETEGKKITNIKEEMEMKRSFESRISIAEKNSLVSMTDTTAFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPDV
Homology
BLAST of Sgr027212 vs. NCBI nr
Match: XP_022144842.1 (E3 ubiquitin-protein ligase ATL42-like [Momordica charantia])

HSP 1 Score: 633.6 bits (1633), Expect = 1.1e-177
Identity = 333/422 (78.91%), Postives = 362/422 (85.78%), Query Frame = 0

Query: 1   MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKF 60
           MSKFSRIIL+I   LL+H NAQ   PPKADSGIP+NLT++IGI+SIMLS+AFILVLYAKF
Sbjct: 1   MSKFSRIILIITATLLVHANAQ-VSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60

Query: 61  CHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH+NS  RG P+AHLG+GLLRSD RFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHRNS--RG-PRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNN 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRISVEDVA+F+ SNSMR MRNN+
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180

Query: 181 QPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVV 240
           Q ELPQDPNMELFVQREEDHHNSSRFNIGSSF KIGK +KEK+   E +     SEE VV
Sbjct: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQ-----SEEHVV 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETE 300
           FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLD+DNIRR  T ETE
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETE 300

Query: 301 GKKITNIKEEMEMKRSFESRISIAEKNSLVSMT-------------------------DT 360
            K+I+NIKEEMEMKRSFE+RISIAEKN+L+S+T                         DT
Sbjct: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDT 360

Query: 361 TAFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQP 398
           TAFSRFGNFNENAIRETSVIE++A+DYRIR+IWLPIARRTVQWF NRE R QLS YTQQP
Sbjct: 361 TAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQP 413

BLAST of Sgr027212 vs. NCBI nr
Match: XP_031738779.1 (putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetical protein Csa_022677 [Cucumis sativus])

HSP 1 Score: 622.9 bits (1605), Expect = 2.0e-174
Identity = 322/422 (76.30%), Postives = 352/422 (83.41%), Query Frame = 0

Query: 1   MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKF 60
           M KFSRII++I+T  L  V AQ P PPKA SGIPQN TIVIGILSIM SM FILV+YAKF
Sbjct: 1   MGKFSRIIMIILTIFLFPVIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  SH RGEPQAHLG GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLS 
Sbjct: 61  CHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSN 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNN 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRI VED+A+F  SNS+R +RNN+
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAFF-KSNSIRLIRNNS 180

Query: 181 QPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVV 240
           + ELPQD  +ELFVQREE+HHNSSR N G+SFR IGK NKEKEVLIENEAG   SEE+V+
Sbjct: 181 RSELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVL 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETE 300
           FHKHKHKILVSDFVFMNRWSNLSSSDL+FLNTEMLG MSSNRFSSLDS++I + ATRE E
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKE 300

Query: 301 GKKITNIKEEMEMKRSFESRISIAEKNSLV--------------------------SMTD 360
           GKK + IKEEMEMKRSFE RISIAEKNSLV                          S++D
Sbjct: 301 GKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDSNASTSRTTILNERRSVSD 360

Query: 361 TTAFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQ 397
           TTAFSRFG FNENA R++S+IEADAK +RIRQ+WLPIARRTVQWF NREQR +LS YTQQ
Sbjct: 361 TTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQ 420

BLAST of Sgr027212 vs. NCBI nr
Match: XP_031742649.1 (E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus])

HSP 1 Score: 609.8 bits (1571), Expect = 1.7e-170
Identity = 314/404 (77.72%), Postives = 341/404 (84.41%), Query Frame = 0

Query: 19  VNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQNSHLRGEPQAHLGLG 78
           V AQ P PPKA SGIPQN TIVIGILSIM SM FILV+YAKFCH  SH RGEPQAHLG G
Sbjct: 7   VIAQAPSPPKAGSGIPQNFTIVIGILSIMFSMVFILVVYAKFCHPTSHFRGEPQAHLGHG 66

Query: 79  LLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFH 138
           LLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFH
Sbjct: 67  LLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFH 126

Query: 139 IGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNNQPELPQDPNMELFVQREE 198
           IGCIDHWLEKHSSCP+CRCRI VED+A+F  SNS+R +RNN++PELPQD  +ELFVQREE
Sbjct: 127 IGCIDHWLEKHSSCPICRCRIGVEDIAFF-KSNSIRLIRNNSRPELPQDSRIELFVQREE 186

Query: 199 DHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVVFHKHKHKILVSDFVFMNR 258
           +HHNSSR N G+SFR IGK NKEKEVLIENEAG   SEE+V+FHKHKHKILVSDFVFMNR
Sbjct: 187 NHHNSSRSNNGTSFRNIGKGNKEKEVLIENEAGECESEERVLFHKHKHKILVSDFVFMNR 246

Query: 259 WSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETEGKKITNIKEEMEMKRSFE 318
           WSNLSSSDL+FLNTEMLG MSSNRFSSLDS++I + ATRE EGKK + IKEEMEMKRSFE
Sbjct: 247 WSNLSSSDLVFLNTEMLGVMSSNRFSSLDSESISQSATREKEGKKTSQIKEEMEMKRSFE 306

Query: 319 SRISIAEKNSLV--------------------------SMTDTTAFSRFGNFNENAIRET 378
            RISIAEKNSLV                          S++DTTAFSRFG FNENA R++
Sbjct: 307 RRISIAEKNSLVSISGISSTSSDPNASTSRTTILNERRSVSDTTAFSRFGYFNENATRDS 366

Query: 379 SVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPD 397
           S+IEADAK +RIRQ+WLPIARRTVQWF NREQR +LS YTQQPD
Sbjct: 367 SMIEADAKSFRIRQLWLPIARRTVQWFANREQRFRLSKYTQQPD 409

BLAST of Sgr027212 vs. NCBI nr
Match: KAE8647036.1 (hypothetical protein Csa_023000 [Cucumis sativus])

HSP 1 Score: 567.8 bits (1462), Expect = 7.5e-158
Identity = 291/376 (77.39%), Postives = 318/376 (84.57%), Query Frame = 0

Query: 47  MLSMAFILVLYAKFCHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLK 106
           M SM FILV+YAKFCH  SH RGEPQAHLG GLLRSDSRFSGVDQKVID+LPFFRFSSLK
Sbjct: 1   MFSMVFILVVYAKFCHPTSHFRGEPQAHLGHGLLRSDSRFSGVDQKVIDALPFFRFSSLK 60

Query: 107 GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAY 166
           GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRI VED+A+
Sbjct: 61  GSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRIGVEDIAF 120

Query: 167 FSNSNSMRFMRNNNQPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLI 226
           F  SNS+R +RNN++PELPQD  +ELFVQREE+HHNSSR N G+SFR IGK NKEKEVLI
Sbjct: 121 F-KSNSIRLIRNNSRPELPQDSRIELFVQREENHHNSSRSNNGTSFRNIGKGNKEKEVLI 180

Query: 227 ENEAGASASEEQVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSL 286
           ENEAG   SEE+V+FHKHKHKILVSDFVFMNRWSNLSSSDL+FLNTEMLG MSSNRFSSL
Sbjct: 181 ENEAGECESEERVLFHKHKHKILVSDFVFMNRWSNLSSSDLVFLNTEMLGVMSSNRFSSL 240

Query: 287 DSDNIRRLATRETEGKKITNIKEEMEMKRSFESRISIAEKNSLV---------------- 346
           DS++I + ATRE EGKK + IKEEMEMKRSFE RISIAEKNSLV                
Sbjct: 241 DSESISQSATREKEGKKTSQIKEEMEMKRSFERRISIAEKNSLVSISGISSTSSDPNAST 300

Query: 347 ----------SMTDTTAFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFV 397
                     S++DTTAFSRFG FNENA R++S+IEADAK +RIRQ+WLPIARRTVQWF 
Sbjct: 301 SRTTILNERRSVSDTTAFSRFGYFNENATRDSSMIEADAKSFRIRQLWLPIARRTVQWFA 360

BLAST of Sgr027212 vs. NCBI nr
Match: KAA0046520.1 (E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa])

HSP 1 Score: 521.5 bits (1342), Expect = 6.2e-144
Identity = 282/420 (67.14%), Postives = 316/420 (75.24%), Query Frame = 0

Query: 1   MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKF 60
           M +F+RII++I+   L  V AQ P PPKA S IPQN TIVIGILSIM SM FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  SH RGEPQA+L  GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNN 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRISVEDVA+F  SNS+R +RNN+
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFF-KSNSIRLIRNNS 180

Query: 181 QPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVV 240
           QP+LPQD +MELFV REE+HH SSR N G+SFRKIGK +KEKEVLIE+EA          
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESEA---------- 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETE 300
                                           EMLGAMSS+RFSSL+S++IRR ATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 301 GKKITNIKEEMEMKRSFESRISIAEKNSLV------------------------SMTDTT 360
           GK+ + IKE MEMKRSFE RISIAEKNSLV                        S++DTT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNERSVSDTT 360

Query: 361 AFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPD 397
           AFSRFG FNENAIR++S+IEADAK YRIRQ+WLPIARRTVQWF NRE+R++LS YTQQPD
Sbjct: 361 AFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQPD 377

BLAST of Sgr027212 vs. ExPASy Swiss-Prot
Match: Q5XF85 (E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 SV=2)

HSP 1 Score: 308.9 bits (790), Expect = 8.2e-83
Identity = 188/440 (42.73%), Postives = 272/440 (61.82%), Query Frame = 0

Query: 6   RIILVIMTNLLLHVNAQGPPPPKAD---SGIPQNLTIVIGILSIMLSMAFILVLYAKFCH 65
           +I    +  L  + +AQ PPP +     +    +L +V G+L+IM ++ F+L++YAK CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 66  ------QNSHLRGEPQAHLGLGLLR---SDSRFSGVDQKVIDSLPFFRFSSLKGSREGLE 125
                      R + +   G+   R   S  RFSG+D+  I+SLP FRFS+LKGS++GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 126 CAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISV-EDVAYFSNSNS 185
           C+VCLSKFE +E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R+S+ ED +  +N NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 186 MRFMRNNNQPELPQDPNMELFVQREEDHH--------NSSRFNIGSSFRKIGK-DNKEKE 245
            RF+   NQ E+ +D ++EL+++REE+           SSRF+IG SFRKI K  NKEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 246 VLIENEAGASASEEQVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRF 305
           +L E+    +  +E+ + HK  H+I+VSD VF NRWSN+SSSDLMFLN+EM+ ++SS RF
Sbjct: 243 LLDEH---VNDKDEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERF 302

Query: 306 SSLDSDNIRRLATRETEGKKITNIKEEMEMKRSFESRI-------------------SIA 365
           SSL  D+++R    +  G  I  IKEEME KR  E+++                   S+ 
Sbjct: 303 SSL--DHVKRGDEEDQIG--ILRIKEEMEAKRMLENKLTSMTTMFSSENGDSGSKSRSVM 362

Query: 366 EKNSLVSMTDTTAFSRF-----GNFNENAIRETSVIE--ADAKDYRIRQIWLPIARRTVQ 398
            +    S++D TA  R      G+ + +A    S ++   +  + R R++WLPIAR+T Q
Sbjct: 363 IEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWLPIARKTAQ 422

BLAST of Sgr027212 vs. ExPASy Swiss-Prot
Match: Q9SL78 (Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 2.6e-76
Identity = 179/407 (43.98%), Postives = 257/407 (63.14%), Query Frame = 0

Query: 8   ILVIMTNLLLHVNAQGPPPPK--ADSGI-PQNLTIVIGILSIMLSMAFILVLYAKFCHQN 67
           IL      L +V+AQ PPPP   A S +   +L I+ G+ SI+ ++ F+L++YAK  H +
Sbjct: 9   ILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHND 68

Query: 68  SHLRGEPQA--------HLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAV 127
             LR E  +         L  GL    SRFSG+D+K I+SLPFFRFS+LKG ++GLEC+V
Sbjct: 69  --LRSETDSDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSV 128

Query: 128 CLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVE-DVAYFSNSN-SMR 187
           CLSKFED+E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R+++E D++   NS+ S+R
Sbjct: 129 CLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLR 188

Query: 188 FMRNNNQPELPQDPNMELFVQREE-DHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGAS 247
            +  +   E  +D  +E++++REE  +  SSRF   SSFRKI K    K +L+E E   +
Sbjct: 189 ILNQSETRE--EDSRLEIYIEREEGTNDGSSRF---SSFRKILK----KSLLLEREGNEN 248

Query: 248 ASEEQVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRR 307
             E++++ HK  H+I+VSD VF NRWSN++SSDL FL +EML ++SS+RFSS+  D + R
Sbjct: 249 IDEKKLM-HKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSV--DRVHR 308

Query: 308 LATRETEGKKITNIKEEMEMKRSFESRISIAEKNSLVSMTDTTAFSRFGNFNENAIRETS 367
              R+         KE+MEMKR    +   + + ++  +T  +     G     +   TS
Sbjct: 309 GNLRD---------KEDMEMKRML-IKHKDSSRRTVSEITTVSREKAVGGSYRGSTASTS 368

Query: 368 ---VIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPDV 398
               + A  ++ R R++WLPIARRT QWFVNRE+   L+   Q  +V
Sbjct: 369 QNYAVTATTEERR-RRLWLPIARRTAQWFVNREKSNDLNTTRQNLNV 390

BLAST of Sgr027212 vs. ExPASy Swiss-Prot
Match: Q5EAE9 (RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2)

HSP 1 Score: 149.1 bits (375), Expect = 1.1e-34
Identity = 102/301 (33.89%), Postives = 152/301 (50.50%), Query Frame = 0

Query: 25  PPPKAD--SGIPQNLTIVIGILSIMLSMAFILVLYAKFC--HQNSHLRGEPQ-------- 84
           PPP+ +  S +   + +VI +L+   S+ F+L+LY K C     S     PQ        
Sbjct: 44  PPPRHNFTSSLMPGIAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYG 103

Query: 85  -AHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLP 144
             +   G +    + SG+D+ VI+SLP FRF +L G ++GLECAVCL++FE  EVLRLLP
Sbjct: 104 GGYYNGGGVVGGRKNSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLP 163

Query: 145 KCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNS---MRFMRNNNQPELPQDP 204
           KCKHAFH+ C+D WL+ HS+CPLCR R+  ED+    + NS   ++F ++ +       P
Sbjct: 164 KCKHAFHVECVDTWLDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDESNSVNNNPP 223

Query: 205 NMELF--VQREEDHHNS-----SRFN---IGSSFRKIGKDNKEK--EVLIENEAGASASE 264
            +  F  V R    H+S     SR N     SS++      +      L  N+AG   SE
Sbjct: 224 GLTRFIPVSRISGRHSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSE 283

Query: 265 EQVV---------------------FHKHKHKILVSDFVFMN-RWSNLSSSDLMFLNTEM 276
              V                       + +H+I++S     + RWS +  SDL++L +EM
Sbjct: 284 SVAVNCLDRLQRKDGLLLIPNRESFEGRFEHRIIISGGNRDDQRWSEVRPSDLLYLRSEM 343

BLAST of Sgr027212 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 3.3e-23
Identity = 58/153 (37.91%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 24  PPPP---------KADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQNSHLRGEPQA- 83
           PPPP          + S     L  +IGIL+  L +     L +K+CH++          
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLN 95

Query: 84  --HLGLGLLRSDSRFS----GVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVL 143
             H G G   S  R S    G+++ +I S+  +++ S  G  +G +C+VCLS+FE+ E L
Sbjct: 96  LNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESL 155

Query: 144 RLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRIS 161
           RLLPKC HAFH+ CID WL+ HS+CPLCR  ++
Sbjct: 156 RLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188

BLAST of Sgr027212 vs. ExPASy Swiss-Prot
Match: Q8W571 (RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3)

HSP 1 Score: 107.1 bits (266), Expect = 4.7e-22
Identity = 60/164 (36.59%), Postives = 89/164 (54.27%), Query Frame = 0

Query: 7   IILVIMTNLLLHVNAQ--GPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQN 66
           IIL +   +    NAQ   P PP   +G   + T V  +L  +  +  +L +Y + C   
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHC--- 73

Query: 67  SHLRGEPQAHLGLGLLRSD---SRFSGVDQKVIDSLPFFRFSSLKGSREG---LECAVCL 126
              R  P +       R++   SR  G+D  V++S P F +SS+K S+ G   LECA+CL
Sbjct: 74  --ARSNPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICL 133

Query: 127 SKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVE 163
           ++ ED E +RLLP C H FHI CID WL  H++CP+CR  ++ +
Sbjct: 134 NELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLTAK 172

BLAST of Sgr027212 vs. ExPASy TrEMBL
Match: A0A6J1CUV0 (E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC111014428 PE=4 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 5.4e-178
Identity = 333/422 (78.91%), Postives = 362/422 (85.78%), Query Frame = 0

Query: 1   MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKF 60
           MSKFSRIIL+I   LL+H NAQ   PPKADSGIP+NLT++IGI+SIMLS+AFILVLYAKF
Sbjct: 1   MSKFSRIILIITATLLVHANAQ-VSPPKADSGIPENLTMLIGIISIMLSLAFILVLYAKF 60

Query: 61  CHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH+NS  RG P+AHLG+GLLRSD RFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHRNS--RG-PRAHLGVGLLRSDLRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNN 180
           FEDIEVLRLLPKCKHAFHIGC+DHWLE HSSCP+CRCRISVEDVA+F+ SNSMR MRNN+
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCVDHWLENHSSCPMCRCRISVEDVAFFTCSNSMRLMRNNS 180

Query: 181 QPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVV 240
           Q ELPQDPNMELFVQREEDHHNSSRFNIGSSF KIGK +KEK+   E +     SEE VV
Sbjct: 181 QSELPQDPNMELFVQREEDHHNSSRFNIGSSFGKIGKGSKEKDGARERQ-----SEEHVV 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETE 300
           FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLD+DNIRR  T ETE
Sbjct: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDTDNIRRSETGETE 300

Query: 301 GKKITNIKEEMEMKRSFESRISIAEKNSLVSMT-------------------------DT 360
            K+I+NIKEEMEMKRSFE+RISIAEKN+L+S+T                         DT
Sbjct: 301 DKRISNIKEEMEMKRSFETRISIAEKNTLISITGMPSISDSNASTSRTINLNERRSVSDT 360

Query: 361 TAFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQP 398
           TAFSRFGNFNENAIRETSVIE++A+DYRIR+IWLPIARRTVQWF NRE R QLS YTQQP
Sbjct: 361 TAFSRFGNFNENAIRETSVIESNAEDYRIRKIWLPIARRTVQWFANREHRAQLSKYTQQP 413

BLAST of Sgr027212 vs. ExPASy TrEMBL
Match: A0A5D3DZA7 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold403G00780 PE=4 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 3.0e-144
Identity = 282/420 (67.14%), Postives = 316/420 (75.24%), Query Frame = 0

Query: 1   MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKF 60
           M +F+RII++I+   L  V AQ P PPKA S IPQN TIVIGILSIM SM FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  SH RGEPQA+L  GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNN 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRISVEDVA+F  SNS+R +RNN+
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFF-KSNSIRLIRNNS 180

Query: 181 QPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVV 240
           QP+LPQD +MELFV REE+HH SSR N G+SFRKIGK +KEKEVLIE+EA          
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGKGSKEKEVLIESEA---------- 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETE 300
                                           EMLGAMSS+RFSSL+S++IRR ATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 301 GKKITNIKEEMEMKRSFESRISIAEKNSLV------------------------SMTDTT 360
           GK+ + IKE MEMKRSFE RISIAEKNSLV                        S++DTT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNERSVSDTT 360

Query: 361 AFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPD 397
           AFSRFG FNENAIR++S+IEADAK YRIRQ+WLPIARRTVQWF NRE+R++LS YTQQPD
Sbjct: 361 AFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQPD 377

BLAST of Sgr027212 vs. ExPASy TrEMBL
Match: A0A1S3CHH4 (E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 PE=4 SV=1)

HSP 1 Score: 499.6 bits (1285), Expect = 1.2e-137
Identity = 272/420 (64.76%), Postives = 304/420 (72.38%), Query Frame = 0

Query: 1   MSKFSRIILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKF 60
           M +F+RII++I+   L  V AQ P PPKA S IPQN TIVIGILSIM SM FILV+YAKF
Sbjct: 1   MGRFNRIIMIILIIFLFPVIAQAPSPPKAGSVIPQNFTIVIGILSIMFSMVFILVVYAKF 60

Query: 61  CHQNSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSK 120
           CH  SH RGEPQA+L  GLLRSDSRFSGVDQKVID+LPFFRFSSLKGSREGLECAVCLSK
Sbjct: 61  CHPTSHFRGEPQANLRHGLLRSDSRFSGVDQKVIDALPFFRFSSLKGSREGLECAVCLSK 120

Query: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNN 180
           FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCP+CRCRISVEDVA+F  SNS+R +RNN+
Sbjct: 121 FEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPICRCRISVEDVAFF-KSNSIRLIRNNS 180

Query: 181 QPELPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVV 240
           QP+LPQD +MELFV REE+HH SSR N G+SFRKIGK                       
Sbjct: 181 QPDLPQDSHMELFVLREENHHYSSRSNDGTSFRKIGK----------------------- 240

Query: 241 FHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETE 300
                                           EMLGAMSS+RFSSL+S++IRR ATRE E
Sbjct: 241 --------------------------------EMLGAMSSSRFSSLESESIRRSATRERE 300

Query: 301 GKKITNIKEEMEMKRSFESRISIAEKNSLV------------------------SMTDTT 360
           GK+ + IKE MEMKRSFE RISIAEKNSLV                        S++DTT
Sbjct: 301 GKRTSQIKEAMEMKRSFERRISIAEKNSLVSISGISSTSDSNASTSKTTNLNERSVSDTT 360

Query: 361 AFSRFGNFNENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPD 397
           AFSRFG FNENAIR++S+IEADAK YRIRQ+WLPIARRTVQWF NRE+R++LS YTQQPD
Sbjct: 361 AFSRFGYFNENAIRDSSMIEADAKSYRIRQLWLPIARRTVQWFANREERSRLSKYTQQPD 364

BLAST of Sgr027212 vs. ExPASy TrEMBL
Match: A0A2N9GBZ7 (RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS24653 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 7.4e-111
Identity = 225/417 (53.96%), Postives = 284/417 (68.11%), Query Frame = 0

Query: 7   IILVIMTNLLLHVNAQGPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQNSH 66
           +++ ++     HV AQ      A    P +LT+VIGILS+M S+  +L++YAKFCH+   
Sbjct: 6   LLIFVLHFFFFHVKAQYTGDGSAS---PGSLTVVIGILSLMFSLTILLLVYAKFCHRRGP 65

Query: 67  LRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEV 126
           +  +PQ      L+RS SRFSG+D+ VI+SLPFFRFSSLKGSR+GLECAVCLSKFED E+
Sbjct: 66  IHSDPQ---NFALIRSSSRFSGIDKTVIESLPFFRFSSLKGSRDGLECAVCLSKFEDTEI 125

Query: 127 LRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNNQPELPQ 186
           LRLLPKCKHAFHI CID+WLEKH+SCPLCR ++S ED + F+ +NSMR +   NQ EL Q
Sbjct: 126 LRLLPKCKHAFHIECIDNWLEKHASCPLCRHKVSAEDPSIFTYTNSMRLI---NQSELRQ 185

Query: 187 DPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVVFHKHKH 246
           D N+ELFVQREEDHH SSRF+IG+SFRK  K +KE+  LI+ EA  S  E  +V HKH H
Sbjct: 186 DSNIELFVQREEDHHGSSRFSIGNSFRKTEKGDKEENPLIQEEADNSFDENHIVLHKHNH 245

Query: 247 KILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETEGKKITN 306
           KI+V DFVF NRWSNLSSSDLMFLN+EMLGA SS RF+S D +N +    RE E   I  
Sbjct: 246 KIIVPDFVFKNRWSNLSSSDLMFLNSEMLGATSSYRFASFDLNNEQSSTARENE--HIVK 305

Query: 307 IKEEMEMKRSFESRISIAEKNSLV----------------------------SMTDTTAF 366
           IKEEM+MKR FE+++S   K S V                            SM++ TA 
Sbjct: 306 IKEEMDMKRLFENKVSTLNKTSAVSFPGIPSTSDSTSNLSQTSRYMNPTERRSMSEITAL 365

Query: 367 SRFGNFN-ENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQ 395
           SR+ +   +N I E S++  + K  R+R++WLPIARRTV W+ NRE+R+Q S  TQQ
Sbjct: 366 SRYEDSGMKNRIGEASLLGNNVKAERMRRLWLPIARRTVHWYANRERRSQQSQNTQQ 411

BLAST of Sgr027212 vs. ExPASy TrEMBL
Match: A0A6P4AKR2 (E3 ubiquitin-protein ligase ATL42 OS=Ziziphus jujuba OX=326968 GN=LOC107431928 PE=4 SV=1)

HSP 1 Score: 406.4 bits (1043), Expect = 1.4e-109
Identity = 228/424 (53.77%), Postives = 290/424 (68.40%), Query Frame = 0

Query: 8   ILVIMTNLLLHVNAQGPPPPKADSGIPQNL----TIVIGILSIMLSMAFILVLYAKFCHQ 67
           I++++  ++ H  AQ   P   ++ IP +L     IVIGIL +M  + F+L++YAK CH+
Sbjct: 22  IILLIIFMISHAEAQA-VPSNYEANIPSDLKPSIAIVIGILCVMFVLTFVLLVYAKLCHR 81

Query: 68  NSHLRGEPQAHLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFED 127
           +  +    Q     GL+   SRFSG+D+ VI+SLPFFRFSSLKGS+EGLECAVCLSKFED
Sbjct: 82  SESVLENLQNQ--QGLISRSSRFSGIDKTVIESLPFFRFSSLKGSKEGLECAVCLSKFED 141

Query: 128 IEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVEDVAYFSNSNSMRFMRNNNQPE 187
            EVLRLLPKCKHAFHI CIDHWLEKHSSCPLCR R+S ED+   + SNSMR +  NNQ E
Sbjct: 142 FEVLRLLPKCKHAFHIDCIDHWLEKHSSCPLCRHRVSSEDLTIIAYSNSMRLLW-NNQSE 201

Query: 188 LPQDPNMELFVQREEDHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGASASEEQVVFHK 247
           L +D N+ELFVQREE H  SSRF+IGSSFRK+ K +KE+E+LI+ E   +  E+Q  FHK
Sbjct: 202 LREDSNIELFVQREEGHRGSSRFSIGSSFRKMEKGDKEEELLIQEEVDEN-EEDQKAFHK 261

Query: 248 HKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRRLATRETEGKK 307
           H HKI+ SDF+F NRWS++SSSDL+FLN+EM+  +SSNRF ++DS+N   L  R +E ++
Sbjct: 262 HNHKIIASDFMFKNRWSSVSSSDLLFLNSEMISHISSNRFPTMDSNNELFLTGRGSENEE 321

Query: 308 ITNIKEEMEMKRSFESRISIAEKNS----------------------------LVSMTDT 367
           I  IKEEMEMKRSFES++S   KNS                              SM++ 
Sbjct: 322 IMKIKEEMEMKRSFESKVSTINKNSGSLSIQVLDPPSDLASSSKTSRAVNQGEKRSMSEI 381

Query: 368 TAFSRFGNFN-ENAIRETSVIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLS-NYTQ 398
           TA SR+GNF+  N I E+       K+  IR++WLPIARRTVQWF NRE+R+ LS   TQ
Sbjct: 382 TALSRYGNFDMSNRIGESHSSRNSVKEESIRRLWLPIARRTVQWFANREKRSHLSQKTTQ 440

BLAST of Sgr027212 vs. TAIR 10
Match: AT4G28890.1 (RING/U-box superfamily protein )

HSP 1 Score: 308.9 bits (790), Expect = 5.8e-84
Identity = 188/440 (42.73%), Postives = 272/440 (61.82%), Query Frame = 0

Query: 6   RIILVIMTNLLLHVNAQGPPPPKAD---SGIPQNLTIVIGILSIMLSMAFILVLYAKFCH 65
           +I    +  L  + +AQ PPP +     +    +L +V G+L+IM ++ F+L++YAK CH
Sbjct: 3   QIFFFFLPLLHSYASAQTPPPFRNGDLVANFEPSLAVVTGVLAIMFALTFVLLVYAKCCH 62

Query: 66  ------QNSHLRGEPQAHLGLGLLR---SDSRFSGVDQKVIDSLPFFRFSSLKGSREGLE 125
                      R + +   G+   R   S  RFSG+D+  I+SLP FRFS+LKGS++GL+
Sbjct: 63  IDLRSGSGDRRRHDRRLRQGIFFNRSTASSDRFSGLDKTAIESLPLFRFSALKGSKQGLD 122

Query: 126 CAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISV-EDVAYFSNSNS 185
           C+VCLSKFE +E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R+S+ ED +  +N NS
Sbjct: 123 CSVCLSKFESVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRDRVSMEEDSSVLTNGNS 182

Query: 186 MRFMRNNNQPELPQDPNMELFVQREEDHH--------NSSRFNIGSSFRKIGK-DNKEKE 245
            RF+   NQ E+ +D ++EL+++REE+           SSRF+IG SFRKI K  NKEK 
Sbjct: 183 FRFL---NQSEIREDSSLELYIEREEEEERIHREELSGSSRFSIGESFRKILKLGNKEKT 242

Query: 246 VLIENEAGASASEEQVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRF 305
           +L E+    +  +E+ + HK  H+I+VSD VF NRWSN+SSSDLMFLN+EM+ ++SS RF
Sbjct: 243 LLDEH---VNDKDEKKLMHKFNHRIVVSDVVFKNRWSNVSSSDLMFLNSEMVNSISSERF 302

Query: 306 SSLDSDNIRRLATRETEGKKITNIKEEMEMKRSFESRI-------------------SIA 365
           SSL  D+++R    +  G  I  IKEEME KR  E+++                   S+ 
Sbjct: 303 SSL--DHVKRGDEEDQIG--ILRIKEEMEAKRMLENKLTSMTTMFSSENGDSGSKSRSVM 362

Query: 366 EKNSLVSMTDTTAFSRF-----GNFNENAIRETSVIE--ADAKDYRIRQIWLPIARRTVQ 398
            +    S++D TA  R      G+ + +A    S ++   +  + R R++WLPIAR+T Q
Sbjct: 363 IEPGRRSVSDITAVPRLSISIHGDCSGSAAETASALQNGGNETEERRRRLWLPIARKTAQ 422

BLAST of Sgr027212 vs. TAIR 10
Match: AT2G20030.1 (RING/U-box superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.8e-77
Identity = 179/407 (43.98%), Postives = 257/407 (63.14%), Query Frame = 0

Query: 8   ILVIMTNLLLHVNAQGPPPPK--ADSGI-PQNLTIVIGILSIMLSMAFILVLYAKFCHQN 67
           IL      L +V+AQ PPPP   A S +   +L I+ G+ SI+ ++ F+L++YAK  H +
Sbjct: 9   ILFFFIIFLDYVSAQSPPPPNLYATSDLFKPSLAIITGVFSIVFTLTFVLLVYAKCFHND 68

Query: 68  SHLRGEPQA--------HLGLGLLRSDSRFSGVDQKVIDSLPFFRFSSLKGSREGLECAV 127
             LR E  +         L  GL    SRFSG+D+K I+SLPFFRFS+LKG ++GLEC+V
Sbjct: 69  --LRSETDSDGERIRHDRLWQGLFNRSSRFSGLDKKAIESLPFFRFSALKGLKQGLECSV 128

Query: 128 CLSKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVE-DVAYFSNSN-SMR 187
           CLSKFED+E+LRLLPKC+HAFHIGCID WLE+H++CPLCR R+++E D++   NS+ S+R
Sbjct: 129 CLSKFEDVEILRLLPKCRHAFHIGCIDQWLEQHATCPLCRNRVNIEDDLSVLGNSSTSLR 188

Query: 188 FMRNNNQPELPQDPNMELFVQREE-DHHNSSRFNIGSSFRKIGKDNKEKEVLIENEAGAS 247
            +  +   E  +D  +E++++REE  +  SSRF   SSFRKI K    K +L+E E   +
Sbjct: 189 ILNQSETRE--EDSRLEIYIEREEGTNDGSSRF---SSFRKILK----KSLLLEREGNEN 248

Query: 248 ASEEQVVFHKHKHKILVSDFVFMNRWSNLSSSDLMFLNTEMLGAMSSNRFSSLDSDNIRR 307
             E++++ HK  H+I+VSD VF NRWSN++SSDL FL +EML ++SS+RFSS+  D + R
Sbjct: 249 IDEKKLM-HKFNHRIVVSDAVFKNRWSNITSSDLTFLTSEMLNSVSSDRFSSV--DRVHR 308

Query: 308 LATRETEGKKITNIKEEMEMKRSFESRISIAEKNSLVSMTDTTAFSRFGNFNENAIRETS 367
              R+         KE+MEMKR    +   + + ++  +T  +     G     +   TS
Sbjct: 309 GNLRD---------KEDMEMKRML-IKHKDSSRRTVSEITTVSREKAVGGSYRGSTASTS 368

Query: 368 ---VIEADAKDYRIRQIWLPIARRTVQWFVNREQRTQLSNYTQQPDV 398
               + A  ++ R R++WLPIARRT QWFVNRE+   L+   Q  +V
Sbjct: 369 QNYAVTATTEERR-RRLWLPIARRTAQWFVNREKSNDLNTTRQNLNV 390

BLAST of Sgr027212 vs. TAIR 10
Match: AT5G05810.1 (RING/U-box superfamily protein )

HSP 1 Score: 143.3 bits (360), Expect = 4.2e-34
Identity = 98/287 (34.15%), Postives = 145/287 (50.52%), Query Frame = 0

Query: 37  LTIVIGILSIMLSMAFILVLYAKFC--HQNSHLRGEPQ---------AHLGLGLLRSDSR 96
           + +VI +L+   S+ F+L+LY K C     S     PQ          +   G +    +
Sbjct: 4   IAVVIAVLTAFFSLTFLLLLYVKHCKRRNGSVYVNHPQRFAITRYGGGYYNGGGVVGGRK 63

Query: 97  FSGVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVLRLLPKCKHAFHIGCIDHW 156
            SG+D+ VI+SLP FRF +L G ++GLECAVCL++FE  EVLRLLPKCKHAFH+ C+D W
Sbjct: 64  NSGIDRSVIESLPVFRFGALSGHKDGLECAVCLARFEPTEVLRLLPKCKHAFHVECVDTW 123

Query: 157 LEKHSSCPLCRCRISVEDVAYFSNSNS---MRFMRNNNQPELPQDPNMELF--VQREEDH 216
           L+ HS+CPLCR R+  ED+    + NS   ++F ++ +       P +  F  V R    
Sbjct: 124 LDAHSTCPLCRYRVDPEDILLIGDCNSWFELQFSKDESNSVNNNPPGLTRFIPVSRISGR 183

Query: 217 HNS-----SRFN---IGSSFRKIGKDNKEK--EVLIENEAGASASEEQVV---------- 276
           H+S     SR N     SS++      +      L  N+AG   SE   V          
Sbjct: 184 HSSAGERASRLNEIRTSSSYKSNPMSFRRSLDSSLKVNDAGEEKSESVAVNCLDRLQRKD 243

BLAST of Sgr027212 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.9 bits (276), Expect = 2.3e-24
Identity = 58/153 (37.91%), Postives = 84/153 (54.90%), Query Frame = 0

Query: 24  PPPP---------KADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQNSHLRGEPQA- 83
           PPPP          + S     L  +IGIL+  L +     L +K+CH++          
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCHRHHQTSSSETLN 95

Query: 84  --HLGLGLLRSDSRFS----GVDQKVIDSLPFFRFSSLKGSREGLECAVCLSKFEDIEVL 143
             H G G   S  R S    G+++ +I S+  +++ S  G  +G +C+VCLS+FE+ E L
Sbjct: 96  LNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVDGSDCSVCLSEFEENESL 155

Query: 144 RLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRIS 161
           RLLPKC HAFH+ CID WL+ HS+CPLCR  ++
Sbjct: 156 RLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFVT 188

BLAST of Sgr027212 vs. TAIR 10
Match: AT4G40070.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.1 bits (266), Expect = 3.4e-23
Identity = 60/164 (36.59%), Postives = 89/164 (54.27%), Query Frame = 0

Query: 7   IILVIMTNLLLHVNAQ--GPPPPKADSGIPQNLTIVIGILSIMLSMAFILVLYAKFCHQN 66
           IIL +   +    NAQ   P PP   +G   + T V  +L  +  +  +L +Y + C   
Sbjct: 14  IILHVAIIIQSKANAQSFSPSPPDLQTGHTPSKTTVFAVLVTLFFLTGLLSVYIRHC--- 73

Query: 67  SHLRGEPQAHLGLGLLRSD---SRFSGVDQKVIDSLPFFRFSSLKGSREG---LECAVCL 126
              R  P +       R++   SR  G+D  V++S P F +SS+K S+ G   LECA+CL
Sbjct: 74  --ARSNPDSSTRYFRNRANDGSSRRGGLDNAVVESFPVFAYSSVKESKIGSKDLECAICL 133

Query: 127 SKFEDIEVLRLLPKCKHAFHIGCIDHWLEKHSSCPLCRCRISVE 163
           ++ ED E +RLLP C H FHI CID WL  H++CP+CR  ++ +
Sbjct: 134 NELEDHETVRLLPICNHLFHIDCIDTWLYSHATCPVCRSNLTAK 172

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022144842.11.1e-17778.91E3 ubiquitin-protein ligase ATL42-like [Momordica charantia][more]
XP_031738779.12.0e-17476.30putative RING-H2 finger protein ATL12 [Cucumis sativus] >KAE8652892.1 hypothetic... [more]
XP_031742649.11.7e-17077.72E3 ubiquitin-protein ligase ATL42-like, partial [Cucumis sativus][more]
KAE8647036.17.5e-15877.39hypothetical protein Csa_023000 [Cucumis sativus][more]
KAA0046520.16.2e-14467.14E3 ubiquitin-protein ligase ATL42-like [Cucumis melo var. makuwa] >TYK28739.1 E3... [more]
Match NameE-valueIdentityDescription
Q5XF858.2e-8342.73E3 ubiquitin-protein ligase ATL42 OS=Arabidopsis thaliana OX=3702 GN=ATL42 PE=1 ... [more]
Q9SL782.6e-7643.98Putative RING-H2 finger protein ATL12 OS=Arabidopsis thaliana OX=3702 GN=ATL12 P... [more]
Q5EAE91.1e-3433.89RING-H2 finger protein ATL43 OS=Arabidopsis thaliana OX=3702 GN=ATL43 PE=2 SV=2[more]
Q9LF643.3e-2337.91RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1[more]
Q8W5714.7e-2236.59RING-H2 finger protein ATL32 OS=Arabidopsis thaliana OX=3702 GN=ATL32 PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1CUV05.4e-17878.91E3 ubiquitin-protein ligase ATL42-like OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A5D3DZA73.0e-14467.14E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
A0A1S3CHH41.2e-13764.76E3 ubiquitin-protein ligase ATL42-like OS=Cucumis melo OX=3656 GN=LOC103500996 P... [more]
A0A2N9GBZ77.4e-11153.96RING-type domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS2465... [more]
A0A6P4AKR21.4e-10953.77E3 ubiquitin-protein ligase ATL42 OS=Ziziphus jujuba OX=326968 GN=LOC107431928 P... [more]
Match NameE-valueIdentityDescription
AT4G28890.15.8e-8442.73RING/U-box superfamily protein [more]
AT2G20030.11.8e-7743.98RING/U-box superfamily protein [more]
AT5G05810.14.2e-3434.15RING/U-box superfamily protein [more]
AT5G17600.12.3e-2437.91RING/U-box superfamily protein [more]
AT4G40070.13.4e-2336.59RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 114..155
e-value: 7.3E-7
score: 38.8
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 113..156
e-value: 1.7E-12
score: 47.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 114..156
score: 12.474524
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 88..164
e-value: 1.2E-21
score: 77.6
NoneNo IPR availablePANTHERPTHR46539:SF1E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 27..390
NoneNo IPR availablePANTHERPTHR46539E3 UBIQUITIN-PROTEIN LIGASE ATL42coord: 27..390
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 113..156
e-value: 1.15906E-20
score: 82.3497
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 93..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr027212.1Sgr027212.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane