Sgr027091 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr027091 vs. NCBI nr
Match: XP_038880237.1 (uncharacterized vacuolar membrane protein YML018C-like [Benincasa hispida])

HSP 1 Score: 508.8 bits (1309), Expect = 3.8e-140
Identity = 281/364 (77.20%), Postives = 307/364 (84.34%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLVRSHFRNRNPKKVAEIQP--PAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSE 120
           VK+ RSHFRN N +KVAE+QP  PA+EER+NQSS  DDDNNNNNNNN        NI+SE
Sbjct: 61  VKIFRSHFRNGNSRKVAELQPPLPALEERTNQSSIEDDDNNNNNNNN--------NIISE 120

Query: 121 MEQREEEGDGDLHCVVHIPSQEQVGV-----NGRCECEAQDVAMA-LKTEERKFTTKEIA 180
           +      G+GD+ CVV+I   E + V     NG CECE +++ M  LKTEE K +TK+IA
Sbjct: 121 V------GNGDVQCVVNIIQGEDLRVNNNNHNGGCECEPENLEMTILKTEETKCSTKQIA 180

Query: 181 VLALTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAV 240
           VLAL IGPI           +ARTSVATTTILFSTSGLFTLIIDACLERQSLTIVN+VAV
Sbjct: 181 VLALVIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNVVAV 240

Query: 241 VVSMAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEE 300
           +VSMAGVAMTTVGKTWARDE  SSSS HGKHS+VGDIFALLSALTDGLYYV+LKKYAGEE
Sbjct: 241 IVSMAGVAMTTVGKTWARDEVRSSSSEHGKHSYVGDIFALLSALTDGLYYVLLKKYAGEE 300

Query: 301 GEKIDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVS 347
           GEK+DMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKF IPQSTKVAE+VLANCF+ NFVS
Sbjct: 301 GEKVDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAELVLANCFVSNFVS 350

BLAST of Sgr027091 vs. NCBI nr
Match: XP_023531720.1 (uncharacterized transporter C405.03c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477.6 bits (1228), Expect = 9.3e-131
Identity = 261/360 (72.50%), Postives = 295/360 (81.94%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWVASAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAFI+ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFIRECI 60

Query: 61  VKLVRSHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEME 120
           VKL RSHF NRN K+VAEIQ              + DNNNNNNN  +N+N+N+N +SE+E
Sbjct: 61  VKLFRSHFPNRNSKEVAEIQ--------------EQDNNNNNNNTNNNNNNNNNNISEVE 120

Query: 121 QREEEGDGDLHCVVHIPSQEQ----VGVNGRCECEAQDVAMALKTEERKFTTKEIAVLAL 180
                 +GD+HCVV+I  +EQ    V  NGRCECEA++      T+E  F+TK++AVLAL
Sbjct: 121 ------NGDVHCVVNIECEEQEKGSVSNNGRCECEAEN-----GTQETPFSTKQMAVLAL 180

Query: 181 TIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSM 240
            IGPI           +ARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVN+VAV VSM
Sbjct: 181 AIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSM 240

Query: 241 AGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKI 300
           AGVAMTTVGKTWARDE   SS GH KHS++GD+FALLSA TDGLYYV+LKKYAGEEGEK+
Sbjct: 241 AGVAMTTVGKTWARDET-QSSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKV 300

Query: 301 DMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
           DMQKFLGYVGLFTLTT+WWLIWPLRA+GIEPKF IPQSTKVAE+VLANCF+ NFVSDYFW
Sbjct: 301 DMQKFLGYVGLFTLTTLWWLIWPLRAMGIEPKFGIPQSTKVAEIVLANCFVSNFVSDYFW 334

BLAST of Sgr027091 vs. NCBI nr
Match: XP_022134812.1 (uncharacterized transporter C405.03c-like [Momordica charantia])

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-130
Identity = 271/363 (74.66%), Postives = 295/363 (81.27%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILLIAVVVIWV+SAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60

Query: 61  VKLVRSHFRNRNPKKVAEI-QPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEM 120
           VKL R+HF N N KKVAE+ QP  MEE+SN  S     NNNNNNNN S            
Sbjct: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPS---SPNNNNNNNNIS------------ 120

Query: 121 EQREEEGDG-DLHCVVHIPSQEQV-----GVNGRCECEAQDVAMALKTEERKFTTKEIAV 180
               E GDG ++HCVV I  +E+V        G CECE Q+VAMALKT+E KF     AV
Sbjct: 121 ----EVGDGSEVHCVVQIQCEEEVVSVDKDNYGGCECENQEVAMALKTKETKF-----AV 180

Query: 181 LALTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVV 240
           LALTIGPI           +ARTSVATTTILFSTSGLFTLIIDA LERQSLTIVNIVAVV
Sbjct: 181 LALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVV 240

Query: 241 VSMAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEG 300
           VS+AGVAMTT+GKTWA+D+ HS+SS HGKHSFVGD FALLSALTDGLYYV+LKKYAGEEG
Sbjct: 241 VSLAGVAMTTIGKTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEG 300

Query: 301 EKIDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSD 347
           EKIDMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKFAIP+STKVAE+VLANCFIGNF+SD
Sbjct: 301 EKIDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSD 339

BLAST of Sgr027091 vs. NCBI nr
Match: XP_022925420.1 (uncharacterized transporter C405.03c-like [Cucurbita moschata])

HSP 1 Score: 468.4 bits (1204), Expect = 5.7e-128
Identity = 256/360 (71.11%), Postives = 291/360 (80.83%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWVASAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  VKLVRSHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEME 120
           VKL RSHF N NPK+VAEIQ              + DNNNNNNNN +N+N     +SE+E
Sbjct: 61  VKLFRSHFPNGNPKEVAEIQ--------------EQDNNNNNNNNNNNNN-----ISEVE 120

Query: 121 QREEEGDGDLHCVVHIPSQEQ----VGVNGRCECEAQDVAMALKTEERKFTTKEIAVLAL 180
                 +GD+HCVV++  +EQ       NGRCECEA++      T+E  F+TK++AVLAL
Sbjct: 121 ------NGDVHCVVNVECEEQEKGSASNNGRCECEAEN-----GTQETSFSTKQMAVLAL 180

Query: 181 TIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSM 240
            IGPI           +A+TSVATTTILFSTSGLFTLI+DAC ERQSL+IVN+VAV VSM
Sbjct: 181 AIGPIWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSM 240

Query: 241 AGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKI 300
           AGVAMTTVGKTWARDEA  SS GH KHS++GD+FALLSA TDGLYYV+LKKYAGEEGEK+
Sbjct: 241 AGVAMTTVGKTWARDEA-QSSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKV 300

Query: 301 DMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
           DMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKF +PQSTKVAE+VLANCF+ NFVSDY W
Sbjct: 301 DMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLW 329

BLAST of Sgr027091 vs. NCBI nr
Match: KAA0050088.1 (putative transporter-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 463.0 bits (1190), Expect = 2.4e-126
Identity = 265/361 (73.41%), Postives = 290/361 (80.33%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLVRSHFRNR-NPKKVAEIQP--PAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVS 120
           +K+VRSHFR + N +KVAEIQ   PA+EER+NQSS       NNNNN  S+   N ++  
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS-------NNNNNTTSDEVENRDVQY 120

Query: 121 EMEQREEEGDGDLHCVVHIPSQEQVGVNGRCE-CEAQDVAM-ALKTEERKFTTKEIAVLA 180
            +   +   +          S      NG CE CEA+++ M  LKTEE KF+TK+IAVLA
Sbjct: 121 VVNINDNNNN----------SNNSSNNNGGCELCEAENMEMRILKTEEAKFSTKQIAVLA 180

Query: 181 LTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVS 240
           LTIGPI           +ARTSVATTTILFSTSGLFTLI+DACLERQSLTIVN VAVVVS
Sbjct: 181 LTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNAVAVVVS 240

Query: 241 MAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEK 300
           MAGV MTTVGKTWA+DE  SSS GHGKHSFVGD FALLSALTDGLYYV+LKKYAGEEGEK
Sbjct: 241 MAGVVMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEK 300

Query: 301 IDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYF 347
           +DMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKF IPQSTKVAEVVLANCF+ NFVSDYF
Sbjct: 301 VDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYF 344

BLAST of Sgr027091 vs. ExPASy Swiss-Prot
Match: Q03730 (Uncharacterized vacuolar membrane protein YML018C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YML018C PE=1 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 5.2e-15
Identity = 92/348 (26.44%), Postives = 152/348 (43.68%), Query Frame = 0

Query: 8   GLILLIAVVVIWVASAEITQSIFTD--YEHPFVVTYVGTSMLVAYLAIAFIKECIVKLVR 67
           GL++L  V+++WV S+ +   IF D  Y  PF +TY  T+  + YL     K  +V    
Sbjct: 16  GLLMLGLVIILWVLSSFLINLIFEDDSYRKPFFITYTNTAAFIFYL-FPTAKAVVVNYKD 75

Query: 68  SHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEMEQREEE 127
           +   N + + + E                            S+SN + ++ S +    E 
Sbjct: 76  TGRANVHRELIME-----------------------EEGTGSDSNRSVDMTSPLLTNLEA 135

Query: 128 GDGDLHCVVHIPSQEQVGVNGRCECEAQDVAMALKTEERKFTTKEIAVLALTIGPI---C 187
           G        H   ++++ +    +  A                 E  +L  T   +    
Sbjct: 136 G-------THANQKKRLTLYETIKLSA-----------------EFCILWFTANLVTNAS 195

Query: 188 MARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSMAGVAMTTVGKTWARDE 247
           +A TSVA+ TIL +TS  FTL I A    +SL+   ++   +S  G+ M T   +  R +
Sbjct: 196 LAFTSVASQTILSTTSSFFTLFIGAICHVESLSKSKVLGSFISFVGIIMVTKSDSHQRYQ 255

Query: 248 AH----SSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKIDMQKFLGYVGLF 307
            H    S          +G++ AL  A+  G+Y  +LK+  G+E  +++M+ F G+VGLF
Sbjct: 256 RHIADVSGDDNDAVQVLIGNLLALAGAVLYGVYSTLLKREVGDE-TRVNMKIFFGFVGLF 312

Query: 308 TLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
            L  +W  +  L   G EP F++P+  KV  ++  NC I  FVSD+ W
Sbjct: 316 NLLFLWPSLIVLDFFGWEP-FSLPKDPKVVVIIFVNCLI-TFVSDFCW 312

BLAST of Sgr027091 vs. ExPASy Swiss-Prot
Match: A6QL92 (Solute carrier family 35 member F5 OS=Bos taurus OX=9913 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-12
Identity = 98/361 (27.15%), Postives = 155/361 (42.94%), Query Frame = 0

Query: 8   GLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECIVKLVRSH 67
           G+++L+ V VIWVAS+E+T  +FT Y  PF  T+  TSM V YL    + +   +     
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYLLGFIVWKPWRQQCTRG 131

Query: 68  FRNRNPKKVAE-----------------IQPPAMEERSNQSSPMDDDNNNNNNNNKSNSN 127
           FR ++    A+                 +  P          P +   N N +  K    
Sbjct: 132 FRGKHATFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPENTNIDTEKIPKK 191

Query: 128 SNSNIVSEMEQREEEGDGDLHCVVHIPSQEQVGVNGRCECEAQDVAMALKTEERK--FTT 187
           S     + ME R+      L   +   S   V      E   + V     T+  K  F  
Sbjct: 192 SRVRFSNIMEIRQLPSSHALEAKLSRMSYPTVKEQ---ESLLKTVGKLTATQVAKISFFF 251

Query: 188 KEIAVLALTIGPICMARTSVATTTILFSTSGLFTLIIDACLERQS---LTIVNIVAVVVS 247
             +  LA       ++ T VA   IL STSGLFTLI+ A     S    T+  ++AV++S
Sbjct: 252 CFVWFLANFSYQEALSDTQVAIVNILSSTSGLFTLILAAMFPSNSGDRFTLSKLLAVILS 311

Query: 248 MAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEK 307
           + GV +  +           S    G+++ +G I++L+ A+   +Y V++K+    E +K
Sbjct: 312 IGGVVLVNLS---------GSEKSPGRNT-IGSIWSLVGAMLYAVYIVMIKRKVDRE-DK 371

Query: 308 IDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYF 347
           +D+  F G+VGLF L  +W   + L   G E  F  P    V   ++ N  IG  +S++ 
Sbjct: 372 LDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIVINGLIGTVLSEFL 416

BLAST of Sgr027091 vs. ExPASy Swiss-Prot
Match: Q8WV83 (Solute carrier family 35 member F5 OS=Homo sapiens OX=9606 GN=SLC35F5 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-12
Identity = 98/371 (26.42%), Postives = 157/371 (42.32%), Query Frame = 0

Query: 8   GLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFI------KECIV 67
           G+++L+ V VIWVAS+E+T  +FT Y  PF  T+  TSM V YL + FI      ++C  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFIIWKPWRQQCTR 131

Query: 68  KLVRSHFR------------NRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNS 127
            L   H                +    + +  P          P +   + N +  K+  
Sbjct: 132 GLRGKHAAFFADAEGYFAACTTDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEKTPK 191

Query: 128 NSNSNIVSEMEQREEEGDGDLHCVVHIPSQEQVGVN-GRCECEAQDVAMALKTEERKFTT 187
            S     + ME R+            +PS   +     R     ++    LKT   K T 
Sbjct: 192 KSRVRFSNIMEIRQ------------LPSSHALEAKLSRMSYPVKEQESILKT-VGKLTA 251

Query: 188 KEIAVLALTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQS---LT 247
            ++A ++     +           ++ T VA   IL STSGLFTLI+ A     S    T
Sbjct: 252 TQVAKISFFFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFT 311

Query: 248 IVNIVAVVVSMAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVIL 307
           +  ++AV++S+ GV +  +          + S        VG I++L  A+   +Y V++
Sbjct: 312 LSKLLAVILSIGGVVLVNL----------AGSEKPAGRDTVGSIWSLAGAMLYAVYIVMI 371

Query: 308 KKYAGEEGEKIDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANC 347
           K+    E +K+D+  F G+VGLF L  +W   + L   G E  F  P    V   ++ N 
Sbjct: 372 KRKVDRE-DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIIING 415

BLAST of Sgr027091 vs. ExPASy Swiss-Prot
Match: Q04083 (Thiamine-repressible mitochondrial transport protein THI74 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=THI74 PE=1 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 5.3e-12
Identity = 86/341 (25.22%), Postives = 155/341 (45.45%), Query Frame = 0

Query: 8   GLILLIAVVVIWVASAEITQSIF--TDYEHPFVVTYVGTSMLVAYLAIAFIKECIVKLVR 67
           G++LL  VVV WV ++ +T  +     Y  PF +TY+  S    YL     +  I++  R
Sbjct: 13  GVLLLAVVVVFWVGASCLTNELLETNAYNKPFFLTYLNISSFALYLTPDLWR--IIQSRR 72

Query: 68  SHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEMEQREEE 127
              + R  + +    P   +E  ++  P+         ++  +++SN + +++ + ++  
Sbjct: 73  KSLQERTERTL----PIHTQESFSEFLPL--------LSSTPSTSSNLSSIADTKVKDTM 132

Query: 128 GDGDLHCVVHIPSQEQVGVNGRCECEAQDVAMALKTEERKFTTKEIAVLALTIGPICMAR 187
               L CV+                                    +A LA       ++ 
Sbjct: 133 RLSLLFCVLWF----------------------------------VANLAAN---AALSY 192

Query: 188 TSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSMAGVAMTTVGKTWARDEAHS 247
           T+VA++TIL STS  FTL +   L  ++ +   ++ + VS+ G+ +  +  +  +D   +
Sbjct: 193 TTVASSTILSSTSSFFTLFLATSLGIETFSTKKLLGLFVSLFGIILIVMQSSKQQDSVSA 252

Query: 248 SSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKIDMQKFLGYVGLFTLTTIWW 307
           SS        VG+  ALL +L   +Y  +LK     +G ++D+Q FLGYVG+FT    W 
Sbjct: 253 SS------FLVGNTLALLGSLGYSVYTTLLKYEISSKGLRLDIQMFLGYVGIFTFLLFWP 294

Query: 308 LIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
           ++  L    +E  F +P +  ++ +V+ NC I  FVSDYFW
Sbjct: 313 ILIILDITHME-TFELPSNFHISFLVMLNCII-IFVSDYFW 294

BLAST of Sgr027091 vs. ExPASy Swiss-Prot
Match: Q5R6J3 (Solute carrier family 35 member F5 OS=Pongo abelii OX=9601 GN=SLC35F5 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 7.0e-12
Identity = 99/363 (27.27%), Postives = 156/363 (42.98%), Query Frame = 0

Query: 8   GLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFI------KECIV 67
           G+++L+ V VIWVAS+E+T  +FT Y  PF  T+  TSM V YL + FI      ++C  
Sbjct: 72  GIVILLLVDVIWVASSELTSYVFTQYNKPFFSTFAKTSMFVLYL-LGFIIWKPWRQQCTR 131

Query: 68  KLVRSH---FRNRNPKKVA---------EIQPPAMEERSNQSSPMDDDNNNNNNNNKSNS 127
            L   H   F +      A          +  P          P +   + N +  K+  
Sbjct: 132 GLRGKHAAFFADAEGYFAACATDTTMNSSLSEPLYVPVKFHDLPSEKPESTNIDTEKTPK 191

Query: 128 NSNSNIVSEMEQREEEGDGDLHCV---VHIPSQEQVGVNGRCECEAQDVAMALKTEERKF 187
            S     + ME R+   +  L      +  P +EQ  +      +      A +  +  F
Sbjct: 192 KSRVRFSNIMEIRQLPSNHALESKLSRMSYPVKEQESI-----LKTVGKLTATQVAKISF 251

Query: 188 TTKEIAVLALTIGPICMARTSVATTTILFSTSGLFTLIIDACLERQS---LTIVNIVAVV 247
               +  LA       ++ T VA   IL STSGLFTLI+ A     S    T+  ++AV+
Sbjct: 252 FFCFVWFLANLSYQEALSDTQVAIVNILSSTSGLFTLILAAVFPSNSGDRFTLSKLLAVI 311

Query: 248 VSMAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEG 307
           +S+ GV +  +          S S        +G I++L  A+   +Y V++K+    E 
Sbjct: 312 LSIGGVVLVNL----------SGSEKSAGRDTIGSIWSLAGAMLYAVYIVMIKRKVDRE- 371

Query: 308 EKIDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSD 347
           +K+D+  F G+VGLF L  +W   + L   G E  F  P    V   ++ N  IG  +S+
Sbjct: 372 DKLDIPMFFGFVGLFNLLLLWPGFFLLHYTGFE-DFEFPNKV-VLMCIIINGLIGTVLSE 415

BLAST of Sgr027091 vs. ExPASy TrEMBL
Match: A0A6J1C0M9 (uncharacterized transporter C405.03c-like OS=Momordica charantia OX=3673 GN=LOC111006993 PE=4 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.9e-131
Identity = 271/363 (74.66%), Postives = 295/363 (81.27%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAW+YKGGLILLIAVVVIWV+SAEITQSIF DYEHPFV+TY+GTSMLVAYLAIAFIKECI
Sbjct: 1   MAWRYKGGLILLIAVVVIWVSSAEITQSIFRDYEHPFVMTYIGTSMLVAYLAIAFIKECI 60

Query: 61  VKLVRSHFRNRNPKKVAEI-QPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEM 120
           VKL R+HF N N KKVAE+ QP  MEE+SN  S     NNNNNNNN S            
Sbjct: 61  VKLFRTHFSNGNSKKVAEVAQPQVMEEKSNNPS---SPNNNNNNNNIS------------ 120

Query: 121 EQREEEGDG-DLHCVVHIPSQEQV-----GVNGRCECEAQDVAMALKTEERKFTTKEIAV 180
               E GDG ++HCVV I  +E+V        G CECE Q+VAMALKT+E KF     AV
Sbjct: 121 ----EVGDGSEVHCVVQIQCEEEVVSVDKDNYGGCECENQEVAMALKTKETKF-----AV 180

Query: 181 LALTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVV 240
           LALTIGPI           +ARTSVATTTILFSTSGLFTLIIDA LERQSLTIVNIVAVV
Sbjct: 181 LALTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLIIDAFLERQSLTIVNIVAVV 240

Query: 241 VSMAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEG 300
           VS+AGVAMTT+GKTWA+D+ HS+SS HGKHSFVGD FALLSALTDGLYYV+LKKYAGEEG
Sbjct: 241 VSLAGVAMTTIGKTWAKDQPHSTSSEHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEG 300

Query: 301 EKIDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSD 347
           EKIDMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKFAIP+STKVAE+VLANCFIGNF+SD
Sbjct: 301 EKIDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFAIPRSTKVAELVLANCFIGNFLSD 339

BLAST of Sgr027091 vs. ExPASy TrEMBL
Match: A0A6J1EHX2 (uncharacterized transporter C405.03c-like OS=Cucurbita moschata OX=3662 GN=LOC111432715 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.7e-128
Identity = 256/360 (71.11%), Postives = 291/360 (80.83%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+AVVVIWVASAEITQSIFTDYEHPFV+TYVGTSMLVAYLAIAF++ECI
Sbjct: 1   MAWKYKGGLILLVAVVVIWVASAEITQSIFTDYEHPFVMTYVGTSMLVAYLAIAFVRECI 60

Query: 61  VKLVRSHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEME 120
           VKL RSHF N NPK+VAEIQ              + DNNNNNNNN +N+N     +SE+E
Sbjct: 61  VKLFRSHFPNGNPKEVAEIQ--------------EQDNNNNNNNNNNNNN-----ISEVE 120

Query: 121 QREEEGDGDLHCVVHIPSQEQ----VGVNGRCECEAQDVAMALKTEERKFTTKEIAVLAL 180
                 +GD+HCVV++  +EQ       NGRCECEA++      T+E  F+TK++AVLAL
Sbjct: 121 ------NGDVHCVVNVECEEQEKGSASNNGRCECEAEN-----GTQETSFSTKQMAVLAL 180

Query: 181 TIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSM 240
            IGPI           +A+TSVATTTILFSTSGLFTLI+DAC ERQSL+IVN+VAV VSM
Sbjct: 181 AIGPIWFVSEYFTNAALAKTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSM 240

Query: 241 AGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKI 300
           AGVAMTTVGKTWARDEA  SS GH KHS++GD+FALLSA TDGLYYV+LKKYAGEEGEK+
Sbjct: 241 AGVAMTTVGKTWARDEA-QSSFGHEKHSYIGDVFALLSAFTDGLYYVLLKKYAGEEGEKV 300

Query: 301 DMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
           DMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKF +PQSTKVAE+VLANCF+ NFVSDY W
Sbjct: 301 DMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFGLPQSTKVAEIVLANCFVSNFVSDYLW 329

BLAST of Sgr027091 vs. ExPASy TrEMBL
Match: A0A5A7U493 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold675G00790 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 1.2e-126
Identity = 265/361 (73.41%), Postives = 290/361 (80.33%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLVRSHFRNR-NPKKVAEIQP--PAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVS 120
           +K+VRSHFR + N +KVAEIQ   PA+EER+NQSS       NNNNN  S+   N ++  
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS-------NNNNNTTSDEVENRDVQY 120

Query: 121 EMEQREEEGDGDLHCVVHIPSQEQVGVNGRCE-CEAQDVAM-ALKTEERKFTTKEIAVLA 180
            +   +   +          S      NG CE CEA+++ M  LKTEE KF+TK+IAVLA
Sbjct: 121 VVNINDNNNN----------SNNSSNNNGGCELCEAENMEMRILKTEEAKFSTKQIAVLA 180

Query: 181 LTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVS 240
           LTIGPI           +ARTSVATTTILFSTSGLFTLI+DACLERQSLTIVN VAVVVS
Sbjct: 181 LTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNAVAVVVS 240

Query: 241 MAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEK 300
           MAGV MTTVGKTWA+DE  SSS GHGKHSFVGD FALLSALTDGLYYV+LKKYAGEEGEK
Sbjct: 241 MAGVVMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEK 300

Query: 301 IDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYF 347
           +DMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKF IPQSTKVAEVVLANCF+ NFVSDYF
Sbjct: 301 VDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYF 344

BLAST of Sgr027091 vs. ExPASy TrEMBL
Match: A0A5D3C4N7 (Putative transporter-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold163G00180 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 2.0e-126
Identity = 265/361 (73.41%), Postives = 291/361 (80.61%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYK GLILL+AVVVIWV SAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI
Sbjct: 1   MAWKYKVGLILLVAVVVIWVTSAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60

Query: 61  VKLVRSHFRNR-NPKKVAEIQP--PAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVS 120
           +K+VRSHFR + N +KVAEIQ   PA+EER+NQSS       NNNNN  S+   N ++  
Sbjct: 61  MKIVRSHFRTKGNCRKVAEIQSPLPALEERTNQSS-------NNNNNTTSDKVENRDVQY 120

Query: 121 EMEQREEEGDGDLHCVVHIPSQEQVGVNGRCEC-EAQDVAM-ALKTEERKFTTKEIAVLA 180
            +   +   +          S      NG CE  EA+++ M  LKTEE KF+TK+IAVLA
Sbjct: 121 VVNINDNNNN----------SNNSSNNNGGCELREAENMEMRILKTEEAKFSTKQIAVLA 180

Query: 181 LTIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVS 240
           LTIGPI           +ARTSVATTTILFSTSGLFTLI+DACLERQSLTIVN+VAVVVS
Sbjct: 181 LTIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACLERQSLTIVNVVAVVVS 240

Query: 241 MAGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEK 300
           MAGVAMTTVGKTWA+DE  SSS GHGKHSFVGD FALLSALTDGLYYV+LKKYAGEEGEK
Sbjct: 241 MAGVAMTTVGKTWAKDEPQSSSYGHGKHSFVGDAFALLSALTDGLYYVLLKKYAGEEGEK 300

Query: 301 IDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYF 347
           +DMQKFLGYVGLFTLTT+WWLIWPLRAIGIEPKF IPQSTKVAEVVLANCF+ NFVSDYF
Sbjct: 301 VDMQKFLGYVGLFTLTTLWWLIWPLRAIGIEPKFMIPQSTKVAEVVLANCFVANFVSDYF 344

BLAST of Sgr027091 vs. ExPASy TrEMBL
Match: A0A6J1L0H0 (uncharacterized vacuolar membrane protein YML018C-like OS=Cucurbita maxima OX=3661 GN=LOC111499921 PE=4 SV=1)

HSP 1 Score: 460.3 bits (1183), Expect = 7.5e-126
Identity = 256/360 (71.11%), Postives = 290/360 (80.56%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           MAWKYKGGLILL+ VVVIWVASAEITQSIF DYEHPFV+TYVGTSMLVAYLAIAF++EC+
Sbjct: 1   MAWKYKGGLILLVVVVVIWVASAEITQSIFMDYEHPFVMTYVGTSMLVAYLAIAFLRECL 60

Query: 61  VKLVRSHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEME 120
           VKL RSHFRN N K+VAEIQ              D DNNNNN NN  N+N+N+N +SE+E
Sbjct: 61  VKLFRSHFRNGNSKEVAEIQ------------EQDKDNNNNNINN--NNNNNNNNISEVE 120

Query: 121 QREEEGDGDLHCVVHIPSQEQ----VGVNGRCECEAQDVAMALKTEERKFTTKEIAVLAL 180
                 +GD+ CVV+I  +EQ    V  N RCECEA++      T+E  F+TK+IAVLAL
Sbjct: 121 ------NGDVLCVVNIECEEQEKGSVSNNDRCECEAEN-----GTQETSFSTKKIAVLAL 180

Query: 181 TIGPI----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSM 240
            IGPI           +ARTSVATTTILFSTSGLFTLI+DAC ERQSL+IVN+VAV VSM
Sbjct: 181 AIGPIWFVSEYFTNAALARTSVATTTILFSTSGLFTLILDACFERQSLSIVNVVAVFVSM 240

Query: 241 AGVAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKI 300
           AGVAMTT+GKTWARDEA SSS  H KHS+VGD+FALLSA TDGLYYV+LKKYAGEEGEK+
Sbjct: 241 AGVAMTTIGKTWARDEAQSSSR-HEKHSYVGDVFALLSAFTDGLYYVLLKKYAGEEGEKV 300

Query: 301 DMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
           DMQKFLGYVGLFTLTT+WWLIWPL+AIGIEPKF IPQSTKVAE+V+ANCF+ NFVSDY W
Sbjct: 301 DMQKFLGYVGLFTLTTLWWLIWPLKAIGIEPKFGIPQSTKVAEIVIANCFVSNFVSDYLW 334

BLAST of Sgr027091 vs. TAIR 10
Match: AT4G32140.1 (EamA-like transporter family )

HSP 1 Score: 257.7 bits (657), Expect = 1.4e-68
Identity = 156/356 (43.82%), Postives = 222/356 (62.36%), Query Frame = 0

Query: 1   MAWKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAIAFIKECI 60
           M W+YK GL L+  VV+IWV SAE+TQ IFT Y+ PF VTY+G S+++ YL +AF+K+ +
Sbjct: 1   MGWRYKAGLFLIGTVVIIWVTSAEVTQDIFTAYKQPFAVTYLGASLMIVYLPVAFLKDWL 60

Query: 61  VKLVRSHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSEME 120
            +     + +R   K            SN+   + DD++    +   +      +   + 
Sbjct: 61  CR-----YLDRRSSK------------SNKIPALTDDSSVELGSPLRHKIIEMGLQGTIT 120

Query: 121 QREEEGDGDLHCVVHIPSQEQVGVNGRCECEAQDVAMALKTEERKFTTKEIAVLALTIGP 180
           +++ E     H       +++  + G+   E Q +      + ++ TTK+IA+  L + P
Sbjct: 121 KKDSEEHLSSH-----EEEDERPLIGKVREEVQTL-----KQRKEITTKQIAMYGLYLAP 180

Query: 181 I----------CMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSMAGVA 240
           I           +ARTSVA+TT+L STSGLFTL I   L + +L +  +VAV VSMAGV 
Sbjct: 181 IWFVTEYLSNAALARTSVASTTVLSSTSGLFTLFIGVFLGQDTLNLSKVVAVFVSMAGVV 240

Query: 241 MTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGEKIDMQK 300
           MTT+GKTWA DE+  +SS +G+ S +GD+F LLSA++ GL+ V+LKK+AGEEGE +D+QK
Sbjct: 241 MTTLGKTWASDESQLNSSLNGERSLMGDLFGLLSAVSYGLFTVLLKKFAGEEGEGVDVQK 300

Query: 301 FLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQSTKVAEVVLANCFIGNFVSDYFW 347
             GY+GLFTL  +WWL+WPL A+GIEPKF IP S KV EVVLAN FIG+ +SDYFW
Sbjct: 301 LFGYIGLFTLVALWWLVWPLTALGIEPKFTIPHSVKVDEVVLANGFIGSVLSDYFW 329

BLAST of Sgr027091 vs. TAIR 10
Match: AT3G07080.1 (EamA-like transporter family )

HSP 1 Score: 71.2 bits (173), Expect = 1.9e-12
Identity = 82/362 (22.65%), Postives = 167/362 (46.13%), Query Frame = 0

Query: 3   WKYKGGLILLIAVVVIWVASAEITQSIFTDYEHPFVVTYVGTSMLVAYLAI----AFIKE 62
           W++  GLI L AV  IW+A++ + QS+      PF++T++  S+ V YL +     ++++
Sbjct: 6   WRWVLGLIYLFAVATIWIAASFVVQSVVEAGVSPFLITFICNSLFVVYLPLFEIGRYLED 65

Query: 63  CIVKLVRSHFRNRNPKKVAEIQPPAMEERSNQSSPMDDDNNNNNNNNKSNSNSNSNIVSE 122
               L+    +  +  ++ E +   +                + +  KS++  +S +V  
Sbjct: 66  AYGSLLFWRSKRSHLMELVESEKAVL-------------LGKDVSGVKSDATESSGLVVR 125

Query: 123 MEQREEEGDGDLHCVVHIPSQEQVGVNGRCECEAQDVAMALKTEERKFTTKEIAVLALTI 182
            ++  E G+G    + +I  +    VN      A  V+     E+ ++T   +A ++L I
Sbjct: 126 EQEISEGGNGIESGLENIELEIDASVN--VSDGASGVSNKGLDEKGRWTRMRVAKVSLVI 185

Query: 183 GP----------ICMARTSVATTTILFSTSGLFTLIIDACLERQSLTIVNIVAVVVSMAG 242
            P          + +  T+V + TIL S S LFT ++      +  T + + +V++ M+G
Sbjct: 186 CPFWFLAQLTFNVSLKYTTVTSNTILSSASSLFTFLVSLIFLGEKFTWLKLFSVLLCMSG 245

Query: 243 VAMTTVGKTWARDEAHSSSSGHGKHSFVGDIFALLSALTDGLYYVILKKYAGEEGE---K 302
             + ++G      ++ S S+   K+  +GDI +L+SA    +Y  +++K   ++ E   +
Sbjct: 246 TIIVSMG------DSESKSNAVAKNPLLGDILSLVSAALYAVYITLIRKKLPDDDERNGR 305

Query: 303 IDMQKFLGYVGLFTLTTIWWLIWPLRAIGIEPKFAIPQST-KVAEVVLANCFIGNFVSDY 347
           + M +FLG++GLF     +  + P   +    +      T K   +V+    + N +SDY
Sbjct: 306 VSMAQFLGFLGLF---NFFIFLPPALILNFTKRERFNALTLKQFGLVVGKGLLDNVLSDY 343