Sgr027021 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr027021
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	isoamylase 1, chloroplastic
Location	tig00153047: 3232350 .. 3249219 (-)
RNA-Seq Expression	Sgr027021
Synteny	Sgr027021

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTGATTGGGAAGGGGATTTACCTCTGCAGCACCCACAAAAAGATCTTATAATATATGAAATGCATGTACGGGGGTTCACCAGGCATGAATCAAGTCATTCAAAATTTCCTGGTACCTATCTTGGTCTAGTGGAGAAGCTAGATTACTTGAAGGTACAGTTTAGAGTGCTTCAATAACAAATCTACCATGCCTTAGATGGGAATATGGTTCTATTCTATTCTCTTTTTATTTTTCAATATTAGACTTTTAATGCTATTAGTGTCCAGAACAAAGAATTCCTGCAAGCATTTGGTGTCAAATAGTTTGCTATTTCTGAGGACTAATTATAATTTTCTCATTAAGGTAGTAAAAGTAGATGTGAAAACACTGGAATTGTTGACACGGTTACTCTCACAGCCCATGTATAAGATTTGTAGTGTATCAGCAAGGGTCTGTAACTGTATGAAATGCAAAACTTCTACTATGAAGGCAAATAAATGGACACTTTTAACCTTCTTTTGAAGTTTTTCTTTCAAATATTTCGAAAGGAGGGGTGTTCGTATCAAAGCTGGCATTTTCTTGAGTTAAAAAAATATACACGTCTCCTAGTGAAATATTGCATTTTGTTTCTGATGGGTTTCCAGTCCATTACTCCATTCATCTTTATTGACTTTGAATCTAATAATTTCTTCATTTAATCTTTTTTTTTTGTTTCTAAATGCAAATTAGAATCGTTGATCTGGCTCTACCTTTTCATTACCTCAAATGTTGCGATCTATAATAAGTAATACATATCCAATTCTTCCATCCTTTTCCTAACGATGTAACACCTTTTTATTTTTTTTATTTCTTTACCTTTCAGGAACTTGGGGTCAACTGTATAGAGTTAATGCCATGCCATGAGTTCAACGAGTTGGAATACTTTAGCTACAACTCTGTGTTGGGTGACTACAAGTACTATTTGATACCTTTATTTGGAGAACTTTCATATTTTTGCTAGTTTAATGCATGGCTTAAACTAGATGGACAAACCGTCTACAATGATAACGGGGAGGGGGAGATTGTTTCCAGAAGTTGAGACTTCCACCTATGTTTATTAAGGGATTGCTTTGACTGCTTGGCTGTTTGGTGCGTGGGAAGCAATGTTTGGCAATGATATTCATATTTGGTTGGGTTAGGATTTAGGACGTTTGAGTTCTCTAACTGAAATGCATGGCTTAGAATTTTCCTCTGAAAAAAGGGCTGAGACCCTTGGACCATTTTTTATTTTGATTTTACACATCCAGTTTTATTTTATAAGAGCACAAGTCACCGCTCTTGAAGATCTGTTATATTGTAACGGCTTTGTTTGGTTTTAATTGGTATGCTGTGAGCAATAAGCTGTATACTCTTTTATACCCAAAATTATACCAATGACTTGAGAAATTAGTTCTATGTTGGAATATTTATGATGGGAAAGTACAAAGGCATCGATATGCCGACTTTCTAGGTTTTTTTTTACCATTTTTGTCATTAAATCATATCATGAAGTAAAAATTTTATCCAGGATGAACTTTTGGGGATACTCAACAGTTGGTTACTTTTCACCAATGATACGATATTCTTCTGCTGGCATACAAAATTGTGGTCAGGATGCAATAAATGAATTCAAGCTTCTTATTAGAGAAGCACATAAGCGTGGAATCGAGGTGGAAGACTGAAATAATTCTATGGTTACTTTTGGTATATTCTGAAATGACTTATTTTTCATTTTTTGTTGGCGTTTCTTTTTTGATCAGGTAATCATGGACGTAGTTTTTAATCACACGGCTGAGGGGAATGAAAATGGCCCAATTTTTTCGTTCAGAGGTGTTGACAATAGTGTGTATTACATGCTTGCACCAATGGTATGTATCAGTAAGATTATTAATTTTCCTTCCTTGCATTTTATTCAGAGGAAAAATAACATAGCATCAATAAAAGCTGGAAGAATGGTGTTTGAGTTCTCTTAATTCAGCTTTCTTCAAAGTTTATGTTATTTTATCTGTATGAGATTTTAATACTGTTGTCAACTGCTTGAAACATCTTTAGACTTCTTTTTAACACAAATTCCTCTTATCTTGTGGTTAATTGTGGAAATGAAAATCCTTAATTTTCTTCAATCTAATGTTTAAGATATCCTGGTGCATGGAAAACCTGAGCAGTCCACTGCTATTGAAAAGAAAAGAAAAGACCAATTCTCTCTTTAGAGTTGATAATTTCTGAAATAAAATGGCAAATGAAGAAATGTTTAGAAACTAACTACTTGTTCTAAGCCTGTGATGTGTGCATTCAAATTATAGAGCCTGGACCTTCACTGTATCTCTTGCTTACACTGGATGGTTGTTTTAATTTTTTCTTTTAAAAATTAAACCGGTATGGTAATAGCACATGCTAAAATTTTAATGGAAGTTCAAGCAATGGGTATTGTCTAGTTTAATTTTTTTTCTTTTTATTTAATTCTTAATGCTTTCTTGTTGAAAAATCTATTTTTCTGTTTTGGATTTTAATTATTTTATAGAATCTTCTCTTTGGTGTTCTCTCTATAAAGAGTTTTGTAATTATGCCAATTGGGAGTTTTTTGTAATATGCTTGGCTTGCGGGATATCTCATCCCCTGGTTTTGTTCATCTTCTCCTTTTGGTCAATAATATTGTTTTGTTTCCTATAAAAAAACAAGATTTTAATTATTTTATGTAGTCTTATCCATGGTTTTGCAGGGAGAGTTTTATAATTATTCGGGATGTGGGAACACATTAACTGCAATCATCCAGCTGTACGTAGATTTATTGTGGACTGCTTGAGGTAGATGTACCATAGTTGTGGATACTATATTTTTGGCATATGTTGGGTTCAGGTTTTTTAAAACTCATGAAAAGAGGTATTACAAAAGGAGAAAGAATATTAGATGTCTTCATGTAGAAGGAATTACAGAGAACTGAACGAAGTAACGGCATTATAAATCAAATCTAAACAACTTGAAATTCCTACCCCAAGAATAAACCAATCTTTTGGCCTTGCCCAAATGTTCTCTATTTTAAACACTCTTTAATTTTTTGATGAAAAACCAAACTTTCGTTATGAGATGGCAATGTAAAAAATAAAAAAATAATAAAAAAAGAGAAAACCCACTGTAAAAGGGGTATGTACAAGGAACTAAACTAAAGATTACAAAAAGGGCTTCAATTTTTGAGAATAAAAGACAAAGGAGTTACAAAACTCTTAACTCCTTAGAGACAGACACCCAGAGACAGCCGACAGGTCTTGGACCTAGCCATAACCCACACCTTTTCCCCAAGATTCTCAACTCCTCTAAAAATTCTCCTATTTCTCGTAAGCTAGATATTCTACAAAATAGCAAAAAAGACTACGCACCAAAAAGCCTCTTCCTTGTCTCAAAATGGTGAGTGAAGGAGGATCTTCCCCATCATCAAATAACATAACTTATTATGAGACAAACATACGCCAAAAGTTAAAAAAAAAAATTGTTCCAATTGAGTATGTGAATTGAAAGCCCCAAAAAACGTGATCTAGACTCTAAAAAATATACTTACACAAAACACATCACTATGGCCCCCTAACAACAAAGAAGAATGCCTTTGAATGCGATTGGTAGGATGTTATGCCATTTGCAATATACGTCTGAGTCCTATGAAATTTTTGGTCGATATCTCTCTTTGATCTCCTTCAAGTAAATGTTACCGCATGGTGATATTGGTTGATATCACAGAGTCACAATGTATGTCCAGCTTTCTAAATCTGAAAATATTCTGCTATCTGCCAGATTATTGCATCTTGTTGGTTTAGTTCATCAACTCTTTTGGCTTTACCTTTGTGGTCATTTCCTCAGTTTCTCCCTCCTCATGCAAGGAGTTGCGATGGCAAGCTCATGCATTTAATCATTCTCATCTTCATAAGGTTTTTCTCCTTGGGAGTAATTTTTTTATTCAAGAAATAAATCAACTGACCACTGCGCTTGTTGTACTTGGACTTGACCTTTGCCTGTGATTGTCTGCATATCCTGCATGTTCAGAACTATTTATTTGGTTTTTGAGATGGACATCTTTGGCTACAGATATTGGGTGATAGAAATGCACGTTGATGGGTTTCGCTTTGATCTTGCTTCTATAATGACCAGAGGTAGCAGGTTATTGGCTGAACTTTCTGTCTGTATACTTAACTTTTGTATGGATATTCCATAAAGAATATATATATTTGTATGGATGTTTGTCTCTTTATTCATCCTTTTCCTCCTTCATGGACAGTCTCTGGGATGCAGTAAATGTGTACGGAAATCCAATAGAAGGTGACGCACTCACAACTGGTTCTCCTCTTGGCAATCCTCCATTAATTGACATGATAAGTAACGACCCAGTACTTCAAGGAGTGAAGGTCTGTACTCTAAATTCCTCTCTTCTACTTGATATGTCTGCTTCATGGACACTTATATTATAAACATATCGTAGATAGTTTTCTTGTTTTTATCTTCTGCTATCAACTGTCCGTTTGAACTCTAATGTTGAATAACTCTCCTAACTCATTAGAGATCAATAATTGGGCAGGTTGCTGCATCTTCTGTTTGATTTAACTTCATAATTCTCTCCTCTCATAATGGTGTAGAATACTTTAAATGCTATGATCTAATATTTTTTTTGAAAGGAAACAGTATTTCATCAATGTAATGAAATGACAAAAGGTAGGGGGACCTCCAAAGCTAATGGGGGTTACAAAAATCCCTTCCAATTAGCACATATAACACCAAGAGAATAATTACAAAAAGATTTATCAAGCTTGCACCAAGTAGCAGCAGTATACAAAATGAGATCAAATAAGTGTAGTTTCTGTCCTTTTCTTTGAAGGTTCTATTGTTCCTTTCAAGCCAAGTGATCCAAAGGAAGCTTCCAATCAAGTTCAGCCGCCGTTTTGAGATAATTGAGCCAAATCAAATGACATAACTCATTTCGAGCCAAATCTTCTCATCATCATATGGATGATGATGTTTGTGTTTTGACCAAAAAGCATAATCGATGTCAAGGACAGTTTCTTCCCATTAGGTTTTTGATATGCTATTGGAAAATTAATGCTTCGGTGATATCAAGGAAATGTATGTTTTTCCTTTCAAGTATTCTATTTTGTTGTACAGTTTGGTGCACGTGAAGTAAATGAAAAATGCCCTCCTTTAAGACATGGTTTAAAGTATTTACTCTCCTTTCTAATATCATGGAAGGAATTTTGTACTGATTGAATTTGGTGCTTACCATCTCATGGAAATGATACTATTCCTGTTCCTTTTGTTGCTATCATTAGGAATTAGTCTTATTCTTTTTCCTGTTTGAAATAGCTCATTGCAGAAGCATGGGATGCTGGGGGCCTTTACCAAGTTGGCACATTCCCTCACTGGGGTGTTTGGTCAGAATGGAACGGAAAGGTCTGTGTAAATATTTTCCCTTTTTGTTTATTATTCCATTATATACTATTTGGTAAAACAAAAGAGAAATTATAAATGATGATAATATGAAGCAATTTGAATATATTTGATATTTCTTTTATATGGGATCAAGAGGTCCTTTGACGACCTTCTTTCACTGGAAAAATGCAAACACCCATGTTATGCACTCAATTAAAGAAGTCGATCTTTTGTAGATCAATTAGTTGCAACAATATTTGGTAATTTTATGGCTTTTGTTCTTTTATCACTGGAAAGAAATTGATGTAGTTGTAGGCTACCTATTTAGCTAGCTTAATGTATAAAGTTTATGTTATTATTTTAGTTTCATTCCAGCTGTTATCTAACTTGTTTTCTGTAAGCTATAAGTTTGTTAGATGTTAAATGGTAAACTGTATTCCTGATGCAGTCAATGCATGTAAAGCGCTGGTTATATTATAAAAAATAGGAATGAAATTAAAAACAGAATCATGATTTTAAACTTCTCTCCCCAGATTGGTATCAGAGTTTCCATTTCCAAAACCAACTGAAATGGTGATCGGGATTTCATCCCAAAGAAAAAAAGGCAATTAGGAAGCTTCTGTCTGAAGTTGGAGATGAAGGATCTATTAGCGATGAGTCTCAGGCGATGTACTTGACTTGTGACAGCTCCTTCCACTGGAATAATTACCACTGAGAGTCGTTGTGATCGAGGGAACCTTGAATGACCTCCAACAAGTGATTCATAACGTTTCTCAATAGGCTATTGCATCGGATGTGGGTAATAATTTCGAAAGAATCAAAGATGAAAACATATTGGCAACTAGAGATGTCATAGTTGGTCATATTGCATGTCAAGCTTGAGTTGATGTAATTCCATAAAGGCTAAAACATATTCCCTAATGTTTGGGTGTTTTTTCTGTTTAGTTCCTATTGTTTTAAAAATTTGGTTCCTTATGTTTCAATTATACCCTTCACGTCGGTGAATGTTAAAATTGGCATATGATAAATTACATGACATTATACTTGGTGAGTTAGTATAAATTTAAGAGAGAAATTAGGCTACCTAAGAGAGGAAAATTATTTTCCTCTAAATTTCAAAGAAGTTGGTAGAAATTTCGATTTTTTTTTCTAGGTGACCTAATTCCTCTTCTAAAGTTCTCCAAACTCACTAAGTATTATGTCATGTAATTTTATCATCCGTCAATTTTAACATTCACCAATGTGAAGGTATAATTGAAAAATGTTGTAACATAAAGGACCACATTGAAACTTTTTAAATATTAGGGATTAAATTGAAAAAACACTTAAACGTTGGGGACCAAAACATATATTTAGCCTAACATTTCTTGAACAGTGAGCTAATAATTATCAAGATAACTGGACACTCTAATTATGAAGATTTCTAAAAGAGGTCAGAAGGACTTTGTTGGAGGATTTTGAAGCAGAGGAAGTCAAAATAAACCAGCTGGGGGAAGAGTGCTTAATTACGAGGATCAAGGTAGCTTGGCCATCATTCAATGGATGATTAGATATAAAAAAAAATTTAGAACTTGGTTAAGGTTGTAAAGAATTATTTTTTATTATGTGAAAATGCCAGAAGAAAGAAAGATGAGGTTAGTAACATATACGTTGGAGGTGTCTCAGCTTGGTCAGATCAATTGCAGTCCAATTTGATGCATGTATAGCCTGCTGTACTGTAATTCTTGGACCTACATGGGGTGATTTATAAAGGAGAGAGACCTCTTCATCAACCATGAACCATTACTGTACGAATCTTTCTAGAACTTCAAGCAAGAGGCTAGAACAGTAGCACCAAAGAGTGGTATTGACTTGATGCTAGAAGCAAAGATTGAATCTGATTACCAACAAGTGCGAGATACATCCATGGGATGAGGGTGGAAATTCAAGATTGTGTAGATTTGCTTCCTTTGGGTGGACTCATTGAGGTCATCCTGCTGGCCCACAAGGTGGAAAAACAAATCAACAAATCAGGGGCAAAAGCTTACCATTCAGGAAATCTCTTGAAATAATTATCATCAACAAACCAAGCAGGTGACTTCTACCAATGGGGCTACTCATAATTATCAAGGTTCAGCATTGGATCAATCTGTTGAAGTGAAACTAAATGTAGATAATAAATTTGAATTTAGTAATGAATGCAATCAGCAACAAAGTGGAAAGAAGAACAGCCCTTATGCTCTCAACCAACTACTGGGAAGTGTTTCTGGTTATGTTTGATGTGGCTTGGGTTCTTTTGCTGTCTCATCCATTGATGCTGCTCGGTTTCCAGGATGTAATTATTTTCGGTGGCTTTAAGTTTTGCAGAAAGTTTTGAGAATTTCGATAGAAGTTTACGAGCAAAAGGAATAGCACAAGGTCTTTGAGGTCATTATTGACAAGGATAGTTGTAAGAAGGTAGTATCAAAGGCATTGTTTAAGGTGCTAGGACTTCATGCCAAGCCTAATCTAAATCCTTAGATTGTTCAATGGACTAAAAAGGGGAATTAATTCCAAGTTACAAAAATCTTCACGGTTTCCTTATTATTGTTAGATATATAAAGACAAAGTGACTTGCAATGTGTTGGAAATGGAGCCTGCCACATTCTATTGGGGAGGTAATGGCAATCTGATGTACAAGGAATTTACAAGGGGAGATATATCTTTGTAGTTTCCAAGGTTTACTAGAAGATTGTTGTTTAGCATCAAATAAAACAAAACTTTCAATTGGAGGCAAAATTTCTCCATATTAATTCCAGTAAAATTTTTTTGTGAAAGATGTACTTGCATTCAGGATTATAGCTCCAGTGGTTAAAGGGTTTGTGAATGGAGAGCTGAATCTTGTACATGATGAGGAGGTACAAACCTTTCTTGAGAGTTTGAGACCTATCCTGCAGATTACATGGTCAACTGCCACAACTTTAAGGAAATCTAGCATTGCATAGACCTGGTTCTTGGTCTCTCTCTCTCTAACCTACCACATTAATGGATGAGCTCAACAAAGTATAAAGTACACCAGGATCAAGTGCAAGACCTCAATAAAGGGTTAATTTGTAATAGCACTAGTCCTCGAACCCAGATTTCTTATGCCTAAGAAGGTGAACTCTTGAAGGATGAGTGTGGATGGTCATGCAATAAACAAGATCACAACTGGGTACCGTTTCCCTATTTTTTAATTGATTGATATACTGGATCAACTTGATAGAGCTCGATTTTTCTTCAAGATTGATTTGGGAAGTGGTTACCACCAATATGTATCTAACCAAGGATGGATGGAAGACTATGTAAAATGAGTGAGAGACTCGTATAATTGCTTGATATGCCTTCTGGATTGTCCAATGTCTAAGCATTTTCATGAAGCTGATGAGTCAGATTCTTCAGCCAGTGGAGCTGTGAAGCAGGAAAGACCGTTGTTAAGAGCAGGATGAGTGAGGAAAGTATTGAGAGGTCTAATCTACGAATTAAACAACCATTAAAGTGAAAAGTTAAAAGTAAAAAAGTGTCGCCTTGCCTAAATATAAAAATAAGGATGACTGGAATTTGTTGGATCTTCAATTAACTAAGTTTGTAACTTTAGCATTTGTCAGCAGTCTTGACGATTCTCAATGTGCTATAACCTATAAGAACCTGTAGTTATGTACTTGCATAATTAGATATTGTTTATATGATTTGGTCACAATTTTTTTTTTTTTTTGTCAATTTCAGTATCGAGACATTGTGCGGCAATTCATCAAAGGCACAAATGGCTTTTCTGGAGCTTTTGCTGAATGCCTTTGTGGGAGCCCTAATCTTTACCAGGTTGAGTTCTCTTTCTTCTCTTTGGTCATATAATGTTACTGTTCTACAGATAGTTTGGTCTGGCCCTGTCAACATTCTGTGGGAAGGGTAACAAAAATAGAATAATAGCTTTGGATATATTTTCTAGAACTAAAGAAAGAGAGTCAGGATTTGGAAGTATTAAACAAAGAAGCTGTGCATTCTTAGCGGCATCCTAATGGAGGTTTGCTTTGGCGCAAGACTTCTTCTGGGGCATGAATATTAGAAACCAGCATAGTGAATATATTGCTAGGGAGTAGGGCTTATTAATGTGTAAAAGCTCTGGAGCTGTGCACTGCACATGATGCAAGGTCCTGCCTGATTCAAGTGGGTGGAATTGTTACAAATTGATTTTTCATTTTTCTACTAAACCATATCATATTATTTTTAACTGTTTATAAGTTTTTTATTATTTGTATATCAATATAAATATTGCTAACGATTACACAAGTATGCAAATATTATTTATGCATATAGTGAATAAATGATGAATCATAGTCTAATTAAATAATAGTCAAAGAAACAAGTATTGTTTATTTGAAGTCTAAACTTGGTTCTACCTTTTGTATTGTAAGTTATAGTGTTATATAGGGTGTTTGCATATTGGGTAGAAAGAATATCGAACAATGTTTTCTAATTTGCATATTCAGTTAAGTATCTTACAATGTTTTTACAGGGAGGAGGAAGGAAACCATGGAATAGTATCAACTTTATTTGTGCACATGATGGTTTCACTTTGGCTGATTTAGTGACTTACAATAGTAAACACAACTTAGCAAATGGTGAAGACAACAAGGATGGTGAAAGTCATAATAATAGCTGGAACTGTGGACAGGTATGCAATCAGTTGTATGCATCTCATAAGATTATCGTTGATTTGGTATGTAAGAGAAGATAATTGGACCTTGTTAACAATGGACATTTGGTGGTACTCCTTTTCATTTCTAGCATTTGCCGGCACATGAAAGACAAATATGTAGTAGATAGGAGCATCAAGATTTCAACAGATATCAAAGATATGCAAGTTCTTTAAAATAAACTAAAGAAAAGAAATTAGGGTTAGTCACCGTGTTCCTTCTCCAAATAGGGATACATTAGGTCTTGTGTATCAAACAGACTGAAAACCTCTTCAATGCCAAAAATTATTGTGTTTGTCAAAGCTACAACAGTTTGGTTTCAATGGTTAAAGCTCAGTTCCACTAATGGCTTCAAAATCTTACTAGAATCCACTGCAATGGGTATGAAGTATCTTGCTGACTCTACTAATGGCGTAAGAAATAACAATTATCATCAGTTTTGAATTTGATCTATAGGCAAGGGGGATTCTTGAAAAAAGGACATGAGAAGGTCGCTAATAAGAATGTCAGCTGCATTGGCAGTTTACAGCAGTTTGAGGACGTGGCACATGACGTTTTTTTGCTAAGCATGCAAAGAGACTATTATCAGGCTGACCTGTCTTTCTTTTTGCTAGTACGTTAGAATGAAGAATTAGTACTTGATCAATGTTTTTATTTGGTTCTTTAGGTCAAAGGAAGAATTAACACTTATGATTTCTTTTCTCCATTTGTTTTTTGTAATCTTTTTCTAAAAGAAAACACAACCTTTTTGTCTCCTTTTGCTGATTTGAAACAACCGTCTACATAAGAAGAAACATACGAAAAGGCAACAAGCAAGATAGGGCATCTCCCAGATCAAGACCGAGGTTACAGAAACACCCTCCAATTGGCATTAATCATAAGGTATAACTAAGAAACATCTTGTTTAGGGCACTCCAATCAAAAGTGGCAAAATGAATAAGATCTTGGAATTCTGGAACTCCTCTAAAGTTTAAAAAAGTCACATGTTCCTTTGAGTCCAAATATTCTATCTTTTCCCACACTATGATGAAATGACTATCTTTTTGGCTTTTTGAGGTCTTTTTCTAGTGCTTTTGTCGTATTTTGCTGTTTGTTTTAAAAATAAAACTAAATTTTTCATTGAATTTGTGAAAAGTTACAACCCTCATTACGTAATAATGACCCCCCTTTAGGACAGGAGTAAAATACACACCCCTAAGAACCCCAAAAGAAAGAAAATGGATATAGAATTCCAACCCAACGAGATCAGAAAAACCCCAAAAGAAACAGGATGGATGACATCCATGAACCAGGAAAAGTTACATCGTATTTTGCTGTTTCGCTGGTACTTTCAGCAATCCTAATAAAAATCTTATTTCTTTTAGGGAGGAGGAGAAAAGATAGAACCACCCTTTGCGTGGAGGATGTTTCCATTAATATGTTCAAGTTGCTTTTATCCAATTAAAGAAAAAAAAAACAGGAGAAATGTGGGGGTGGTAATTAGAGTTTCTCGCCATATAAATTTCTGAATTTACTCTATCCATATACTCTAGTACTCCAAGGTTGTCATCTAGAGAATAAGAACACACATCAAAGTTTGTTTTTTCTAAAAGGATATTTGCATCAAATTTTCATGAGAGAATAGGAGTATTGTTGCCTGAAAGTCTTTCCTGTTTTGTGTTCAATGGGTTGAGTACTAAAGGCTCCTAAGTTTTATTTATTAGGATGAGCATTAATCTTTCTATATGGGTTTCTCCTGATAGCTTTTTATACCTTTTTGGAGCCTGTCATCTAGATAAATCCGTTTGTTTTTGGGTTTTGTTTATTGTTACTTATTGCAATGGGCTTTTTTTTCTTCCTTAATGTATGTCTGTTTCCTACATTACACAGGAGGGAGAGTTTGTTAGCATCTCTGTAAAGAAATTGAGGAAAAGACAAATGCAAAATTTCTTTGTCTGCCTCATGGTTTCCCAAGTAAGATTCGCTGATATAATCTATGGTATGTTAATTCTTTACGTTGAGAAAATTAATGCACAAAAAATTTTCTTGCCCATATCATACTGGATATGTCAGTCAGATTATAGGGGCATCACATTTTCTTTCATGGACTTGTACGAGCATTGTTCTCTGGGTAAGACATTGCTCTAGTTGTGTGCCAGATATTCCATCATAAGCTAGATACTCATTCAAAGTTTAGCTGTGTATGCCTTGTTTACAGCAATAATATTTACTCCCTTGAAGGTAATCATTTTTGGCTTACTTCCCCAAATTTTTTATAAAATCAAGGTTGCACTTGCATACCGGAATCCAACAGCCGTTCACATGTAGGAAATCCTGATGATACCTTGGTATATAAAATTAACGTGTTTATTTTAAAAATTTGTTTATAGGAATCTCATAAGGATGATAAGAAACATTCCAATATCATCGATGAGTTAAGAAGAATTAATTTGAAATAATTTTATATAAATATTGGCATGTGAAATTAACATAAATTTATTTAAGATGTTAATTTGTTATAGACCATATATTATATTTTTTATAACATTTGTGGCAAGGAAGCACAAATATCAATTTGTAATGAGAATTCAAGAAATCTTCCAAATTTTGGTGAGAATAAAAAGCCTCTTAGATGCCTTTTATCTTAAGATACCCGACATTCTGATCTGTCTGCAAAACGAACATGAGAATTTGGATACCTGAAGGTGGGTATCCAAAGATTCCCACAAAATAGAACCTGACAACTTGATGTCACTACCAAGCATTTTAATTTGTGGTGGAATAAAAATGTGGTTTATTTGTGCTTCAATAGTATAATTAATTTAAGTCTGAACCAGTTTCTTTTCCCTCGATGTATTTTTAACACTAACTCCTGGTTTACTTGTGTGGGTGTCTGGAAACTTCTCCCTATATGCGCTCATGCATTCTTGTAATATGATAGGATATGATATTCATGCACATAGCATATGTAATCAGGCCATAGTTTAGCATGTTACACCACTGGCAACATTTATTTCACCATAGTTTCTCTCTACTGTCATAATAAGTCCAAAAACACGTGTAATAGAATGTTTCTATCCAACATTAGTTCTCTAAAATTTGTTTGACTGTTTCTTATCTGATCAGGGTGTCCCAATGATATTTATGGGTGATGAATATGGTCACACAAAGGGAGGGAACAACAATACATATTGCCATGATAACTATGTAGTTCATTCATCTTGCTATTTTCTGACCTTTTTTGAAATTCAATTCTGTTGACCTGAGCTGCTAACTTTGCTACAGATCAATTATTTCCGTTGGGATAAGATAGAAGAAGAGTCATCATCTTACTTCCATAGGTTCTGCAGTCTTATGACCACATTCCGTTAGTAAGTGCCTCCTTTCTGAATTAATTTGTTTCGTTTAGTTCAGTGTTCTAAATGACTATTTATCCTCTCTTGATGCCTTTTTTGTATGATCTAGCAGTGAATGTGAATCACTTGGCTTAAATGATTTCCCAACAGCTGAGAGACTGCAATGGCATGGTCATTCTCCTGGAAAGCCAAACTGGTCTGCAGCAAGTCGTTTTGTGGCCTTCTCTCTGGTATGTTTCCTTTTCCAAATAATATTTGCTCACACAAACCAATCTTTCTCCTTCTGTTTTCTAATGTGGTATTTCGGCCAAGTTATCTTATTCTTCATCCTTTTGATCTCTCTGTATTCAACATCGGTTCCCTAACACATTGCTTTTTAACTTACTGGGATCATGTCTGTTGTTGTATACTCAAGAATAATTGATTCTTATTTGGAGTGGCTTGAACCCAGGTTGATTCAGTAAAAGGAGAAATTTACATTGCTTTCAATGCCAATCATTTACCCGTCACCGTGACATTGCCCGAGAGGCCTGGATACAGATGGGAACCATTGGTAGACACGGGTAAGATATCGCCATTTGACTTTCTCTCAAGTGATCTTCCCGAAAGAGATGTGGCAATCCGACAGTACGCTCACTTCCTCGACGCCAATTATTATCCCATGCTTAGTTATTCTTCCATCGTTCTTCTACTCTCTCCAGAAGAACATGCATCATCCTGATCATCTTCATATCCCATAAACCCTTAAAAGACTGCTTCTTCTGGTGTGTGTGTGTATATTTTATATCCATACACAGTGGGATGTCTGTGATATTTATTTCTTTATATTTCATATGACCATCCTTGCATATAAACATGGTTCGAAATCAGTAAACTTGAGTAATTTTGTGTTGTAGTTTGTGATATGGCTATGCTTGTCCATTCAGAACTAAATTTATTATTGCATTTGAGGAAATGCTATATTATTTGATTGGGAGCTGTTGCCGGCCATTTTGCTTAGTAGGGCATTTTTGAAAAATGTGTTAAGAAAGGGTTGATTTTAAAATTTTTTTTTAAGAATATAATATTTCAAGTGTGGGAGCGGGGATATGAACCTTGGATCTATAAGAGGGGCTATTAATGTCTTAATTAGTTGAGTTATTGTTTGGTGATGAAAATATATAATTTTGATATATTTATTAATAATTAATTATTTTTTATTTTAAGAATAATTTAAAATTAAAATGAGATATGGATTTTTTTTTTTTCATGGGAAAGTCTACTGTTGAGTGGAGATTTCTCAGCCCACCTTGATCTCAATTCTCGAGAAAATGAGGTGGGAATGAGGAATAAAAATGCTTGTGGGGTTGAGATAGATAATGCATCTTTCCTTCTTTGCCCTGTCTTGCATTCTAAGTTTCGTTTTCTTTTTTCTTTTCCTTTTTTTTTTTTACTTGCACTCATCTAATGAACAATCTGGATCTTTCCTATAAAAGTAACTTAATTGTGAATTTGCTTTGTTATATTCTATGGCTCAATGCAATTATCTTGTAACTTTTTCATAGTGGAAAGTTAAAATTTCATAATTACTCATCCAAGAACTGTACAGTAATATCAGAATGGATATCCTTATTGTGATGAGGAATAATCATAGAAAAACCAAAGCAGAAAGCCATTTTCATATCAAATAGCAAAAAGATATGGATGTTTTAAAAGTACAGGACCAATCAGATTTAACCTTTAATTTACATATAGAACAAAATAATATTAAACAAGTTTGTTTTTTTCTAAATTAGAAAATAAATTCAAAGAATTAACAAATAAGCATCTAAATAGAAGTTAATAATTTTTCGCACATGTCAGACTGAGGAAGGAAGCGGGAAAATGCGATGAAATCCCTTTTCCATCCAATCGTAAAACGCCACATTGCAAAAAATTCTGATTAACCGCTAATACAGTTATATACTATATAAAGATTAAAAATTAAAAAAAAAAAAAAAAAAGGTGAATTGGGCTTCTTCTTCTCTATCAGTGGGGCTTTCCAGAGGCTCTAGAGACGACCAGAAGCCATTTTTCTTCTCTCTCAGTGGGCTTCTCTCTCTTAACAATTTTCTGCTGTTTTTCCTTCTCTTGTTTATATAAAGCTAGCAATCCATTTTTGCTTCAGCGAAGTTGGTCGGAAGGGAATCTGATTTTTCATCGAGGGGTTCTATTTTCTGTGGTGGGAAGGAAGCTGTGTGGAGTTATTAACAGTAGAGATTTGGAAGAGTCGTTTTTCGTTTGATCGGAGATGAAGATGAAGATGAAAAGAAAAGATCTCGATCAATTTAACGATGAGTTCTCGGATTTCTCTCTCTCGTCGCCTGCTAGGAAGATTCGCCGTCTGGTACTTTTTACTTCGGTGTCATTTCTATCTATGGAGCTTTAGGGCTTTTTTCTAAGAATTTTTTGCGATTTTCTAGGATGTTGGTTTGCCGCCTATTATAGAAGAGGAAGAGCAGCCGGAGATTGCTGTTTTAAGCGAAGAGCCATTGATGCTGAGAATATTATAGTTGGTGGAGGCGGTCTGAGGATTGAGGAATTGCCAGACGCTTCCTCTGTCTCTGCTTCAGCTTGTGTTATGGAAGATCGTGCTTTCTGCGACAACAAGAGAGGGCTATTGTTCTCTTTAAGCCTGTTAACACGCCTTTCTTTCAGTCATCTCCTCTTTCGGTCTCTGTCGATTCCGACATTATATCTGGCTTCAAGAGTAAGTACTCATTTCAACGTAGTTTCTACATATTTATCTCTCTTCCGATCTTTTAATCCTACACTTAAGCTACCTAGCTTCTAAGCTTGTCTGCTCTGGGGTTTCCAAATTTTTTTTTTGGGCTGTTTGATATTGGACAAAGGAATGTCTGCGTAGGCATCACGTAGACGCTCGAGTAGCATAGATTTAGACTACAAAATTAGTTTAATAGCTTTTCTTAAAGGGGTTAAATATGAAAGACTAGACGTAGGTTTCACCCATTCTGGTTTGGCAAAGAGTAATTATTAAGTGTTCGTATAGTTATTGTCCGAAAATGGACCATGAAGAATGATTCTGATCTCCATGGATCTGAAAATTATCAGACGGAATTCTTCGTGAAAACCCTTATGGTGGCCGGCTGAAATTTGGTGAGGATGACGACGAAATGGGGACTGAGAACAAAAATCTAGCTGTTGTTCCCTGGATTCCGCGGCTACAGGTTCCCGCTCCCACAAACATGCATGTGCCCCTAGAGGAGGCTCCACAGTTGATGGAAGCTGAAGACGTTGGAGAGGCAACAATGGACATTGAAGATGACAGCTTGAGCAGTCATCAACCTTATGGATATGGTGGAATGGACGGCGGTAATGCTGTACATCAATGGCACCAACAGCACTGCATGATCCCACAGCTGCCGCAGCAGACGTCCACACCCATATCATGGTTCCGTTGA

mRNA sequence

TTTGATTGGGAAGGGGATTTACCTCTGCAGCACCCACAAAAAGATCTTATAATATATGAAATGCATGTACGGGGGTTCACCAGGCATGAATCAAGTCATTCAAAATTTCCTGGTACCTATCTTGGTCTAGTGGAGAAGCTAGATTACTTGAAGGAACTTGGGGTCAACTGTATAGAGTTAATGCCATGCCATGAGTTCAACGAGTTGGAATACTTTAGCTACAACTCTGTGTTGGGTGACTACAAGATGAACTTTTGGGGATACTCAACAGTTGGTTACTTTTCACCAATGATACGATATTCTTCTGCTGGCATACAAAATTGTGGTCAGGATGCAATAAATGAATTCAAGCTTCTTATTAGAGAAGCACATAAGCGTGGAATCGAGGTAATCATGGACGTAGTTTTTAATCACACGGCTGAGGGGAATGAAAATGGCCCAATTTTTTCGTTCAGAGGTGTTGACAATAGTGTGTATTACATGCTTGCACCAATGATTATTGCATCTTGTTGGTTTAGTTCATCAACTCTTTTGGCTTTACCTTTGTGGTCATTTCCTCAGTTTCTCCCTCCTCATGCAAGGAGTTGCGATGGCAAGCTCATGCATTTAATCATTCTCATCTTCATAAGAACTATTTATTTGGTTTTTGAGATGGACATCTTTGGCTACAGATATTGGGTGATAGAAATGCACGTTGATGGGTTTCGCTTTGATCTTGCTTCTATAATGACCAGAGGTAGCAGTCTCTGGGATGCAGTAAATGTGTACGGAAATCCAATAGAAGGTGACGCACTCACAACTGGTTCTCCTCTTGGCAATCCTCCATTAATTGACATGATAAGTAACGACCCAGTACTTCAAGGAGTGAAGCTCATTGCAGAAGCATGGGATGCTGGGGGCCTTTACCAAGTTGGCACATTCCCTCACTGGGGTGTTTGGTCAGAATGGAACGGAAAGTATCGAGACATTGTGCGGCAATTCATCAAAGGCACAAATGGCTTTTCTGGAGCTTTTGCTGAATGCCTTTGTGGGAGCCCTAATCTTTACCAGGGAGGAGGAAGGAAACCATGGAATAGTATCAACTTTATTTGTGCACATGATGGTTTCACTTTGGCTGATTTAGTGACTTACAATAGTAAACACAACTTAGCAAATGGTGAAGACAACAAGGATGGTGAAAGTCATAATAATAGCTGGAACTGTGGACAGGAGGGAGAGTTTGTTAGCATCTCTGTAAAGAAATTGAGGAAAAGACAAATGCAAAATTTCTTTGTCTGCCTCATGGTTTCCCAAGGTGTCCCAATGATATTTATGGGTGATGAATATGGTCACACAAAGGGAGGGAACAACAATACATATTGCCATGATAACTATATCAATTATTTCCGTTGGGATAAGATAGAAGAAGAGTCATCATCTTACTTCCATAGGTTCTGCAGTCTTATGACCACATTCCGTTATGAATGTGAATCACTTGGCTTAAATGATTTCCCAACAGCTGAGAGACTGCAATGGCATGGTCATTCTCCTGGAAAGCCAAACTGGTCTGCAGCAAGTCGTTTTGTGGCCTTCTCTCTGGTTGATTCAGTAAAAGGAGAAATTTACATTGCTTTCAATGCCAATCATTTACCCGTCACCGTGACATTGCCCGAGAGGCCTGGATACAGATGGGAACCATTGGTAGACACGGGTAAGATATCGCCATTTGACTTTCTCTCAAGTGATCTTCCCGAAAGAGATGTGGCAATCCGACAAAGAGGAAGAGCAGCCGGAGATTGCTGTTTTAAGCGAAGAGCCATTGATGCTGAGAATATTATAGTTGGTGGAGGCGGTCTGAGGATTGAGGAATTGCCAGACGCTTCCTCTGTCTCTGCTTCAGCTTGTGTTATGGAAGATCGTGCTTTCTGCGACAACAAGAGAGGGCTATTGTTCTCTTTAAGCCTGTTAACACGCCTTTCTTTCAGTCATCTCCTCTTTCGGTCTCTGTCGATTCCGACATTATATCTGGCTTCAAGAGCATCACGTAGACGCTCGAGTAGCATAGATTTAGACTACAAAATTAGTTTAATAGCTTTTCTTAAAGGGGTTAAATATGAAAGACTAGACGTAGGTTTCACCCATTCTGGTTTGGCAAAGAACGGAATTCTTCGTGAAAACCCTTATGGTGGCCGGCTGAAATTTGGTGAGGATGACGACGAAATGGGGACTGAGAACAAAAATCTAGCTGTTGTTCCCTGGATTCCGCGGCTACAGGTTCCCGCTCCCACAAACATGCATGTGCCCCTAGAGGAGGCTCCACAGTTGATGGAAGCTGAAGACGTTGGAGAGGCAACAATGGACATTGAAGATGACAGCTTGAGCAGTCATCAACCTTATGGATATGGTGGAATGGACGGCGGTAATGCTGTACATCAATGGCACCAACAGCACTGCATGATCCCACAGCTGCCGCAGCAGACGTCCACACCCATATCATGGTTCCGTTGA

Coding sequence (CDS)

TTTGATTGGGAAGGGGATTTACCTCTGCAGCACCCACAAAAAGATCTTATAATATATGAAATGCATGTACGGGGGTTCACCAGGCATGAATCAAGTCATTCAAAATTTCCTGGTACCTATCTTGGTCTAGTGGAGAAGCTAGATTACTTGAAGGAACTTGGGGTCAACTGTATAGAGTTAATGCCATGCCATGAGTTCAACGAGTTGGAATACTTTAGCTACAACTCTGTGTTGGGTGACTACAAGATGAACTTTTGGGGATACTCAACAGTTGGTTACTTTTCACCAATGATACGATATTCTTCTGCTGGCATACAAAATTGTGGTCAGGATGCAATAAATGAATTCAAGCTTCTTATTAGAGAAGCACATAAGCGTGGAATCGAGGTAATCATGGACGTAGTTTTTAATCACACGGCTGAGGGGAATGAAAATGGCCCAATTTTTTCGTTCAGAGGTGTTGACAATAGTGTGTATTACATGCTTGCACCAATGATTATTGCATCTTGTTGGTTTAGTTCATCAACTCTTTTGGCTTTACCTTTGTGGTCATTTCCTCAGTTTCTCCCTCCTCATGCAAGGAGTTGCGATGGCAAGCTCATGCATTTAATCATTCTCATCTTCATAAGAACTATTTATTTGGTTTTTGAGATGGACATCTTTGGCTACAGATATTGGGTGATAGAAATGCACGTTGATGGGTTTCGCTTTGATCTTGCTTCTATAATGACCAGAGGTAGCAGTCTCTGGGATGCAGTAAATGTGTACGGAAATCCAATAGAAGGTGACGCACTCACAACTGGTTCTCCTCTTGGCAATCCTCCATTAATTGACATGATAAGTAACGACCCAGTACTTCAAGGAGTGAAGCTCATTGCAGAAGCATGGGATGCTGGGGGCCTTTACCAAGTTGGCACATTCCCTCACTGGGGTGTTTGGTCAGAATGGAACGGAAAGTATCGAGACATTGTGCGGCAATTCATCAAAGGCACAAATGGCTTTTCTGGAGCTTTTGCTGAATGCCTTTGTGGGAGCCCTAATCTTTACCAGGGAGGAGGAAGGAAACCATGGAATAGTATCAACTTTATTTGTGCACATGATGGTTTCACTTTGGCTGATTTAGTGACTTACAATAGTAAACACAACTTAGCAAATGGTGAAGACAACAAGGATGGTGAAAGTCATAATAATAGCTGGAACTGTGGACAGGAGGGAGAGTTTGTTAGCATCTCTGTAAAGAAATTGAGGAAAAGACAAATGCAAAATTTCTTTGTCTGCCTCATGGTTTCCCAAGGTGTCCCAATGATATTTATGGGTGATGAATATGGTCACACAAAGGGAGGGAACAACAATACATATTGCCATGATAACTATATCAATTATTTCCGTTGGGATAAGATAGAAGAAGAGTCATCATCTTACTTCCATAGGTTCTGCAGTCTTATGACCACATTCCGTTATGAATGTGAATCACTTGGCTTAAATGATTTCCCAACAGCTGAGAGACTGCAATGGCATGGTCATTCTCCTGGAAAGCCAAACTGGTCTGCAGCAAGTCGTTTTGTGGCCTTCTCTCTGGTTGATTCAGTAAAAGGAGAAATTTACATTGCTTTCAATGCCAATCATTTACCCGTCACCGTGACATTGCCCGAGAGGCCTGGATACAGATGGGAACCATTGGTAGACACGGGTAAGATATCGCCATTTGACTTTCTCTCAAGTGATCTTCCCGAAAGAGATGTGGCAATCCGACAAAGAGGAAGAGCAGCCGGAGATTGCTGTTTTAAGCGAAGAGCCATTGATGCTGAGAATATTATAGTTGGTGGAGGCGGTCTGAGGATTGAGGAATTGCCAGACGCTTCCTCTGTCTCTGCTTCAGCTTGTGTTATGGAAGATCGTGCTTTCTGCGACAACAAGAGAGGGCTATTGTTCTCTTTAAGCCTGTTAACACGCCTTTCTTTCAGTCATCTCCTCTTTCGGTCTCTGTCGATTCCGACATTATATCTGGCTTCAAGAGCATCACGTAGACGCTCGAGTAGCATAGATTTAGACTACAAAATTAGTTTAATAGCTTTTCTTAAAGGGGTTAAATATGAAAGACTAGACGTAGGTTTCACCCATTCTGGTTTGGCAAAGAACGGAATTCTTCGTGAAAACCCTTATGGTGGCCGGCTGAAATTTGGTGAGGATGACGACGAAATGGGGACTGAGAACAAAAATCTAGCTGTTGTTCCCTGGATTCCGCGGCTACAGGTTCCCGCTCCCACAAACATGCATGTGCCCCTAGAGGAGGCTCCACAGTTGATGGAAGCTGAAGACGTTGGAGAGGCAACAATGGACATTGAAGATGACAGCTTGAGCAGTCATCAACCTTATGGATATGGTGGAATGGACGGCGGTAATGCTGTACATCAATGGCACCAACAGCACTGCATGATCCCACAGCTGCCGCAGCAGACGTCCACACCCATATCATGGTTCCGTTGA

Protein sequence

FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLALPLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCSLMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNANHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQRGRAAGDCCFKRRAIDAENIIVGGGGLRIEELPDASSVSASACVMEDRAFCDNKRGLLFSLSLLTRLSFSHLLFRSLSIPTLYLASRASRRRSSSIDLDYKISLIAFLKGVKYERLDVGFTHSGLAKNGILRENPYGGRLKFGEDDDEMGTENKNLAVVPWIPRLQVPAPTNMHVPLEEAPQLMEAEDVGEATMDIEDDSLSSHQPYGYGGMDGGNAVHQWHQQHCMIPQLPQQTSTPISWFR

Homology

[Close]

BLAST of Sgr027021 vs. NCBI nr
Match: XP_022134724.1 (isoamylase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 1059.3 bits (2738), Expect = 1.7e-305
Identity = 508/585 (86.84%), Postives = 526/585 (89.91%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQ+PQKDLIIYEMHVRGFTRHESSHS+FPGTYLGLVEKLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQYPQKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVG+FSPMIRYSSAGI+ CGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPM               
Sbjct: 346 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWV EMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVTEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHWGVWSEWNG+YRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYN+KHNLANGEDN DGESHNNSWNCGQEGEFVS+SVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNYINYFRWDK+ EESSS FHRFCS
Sbjct: 646 RNFFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKL-EESSSDFHRFCS 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           L+TTFR+ECESLGLNDFPTAERLQWHG+SPGKP+WS ASRFVAF+LVDSVKGEIYIAFNA
Sbjct: 706 LVTTFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ
Sbjct: 766 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 779

[Close]

BLAST of Sgr027021 vs. NCBI nr
Match: XP_022932124.1 (isoamylase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1042.0 bits (2693), Expect = 2.8e-300
Identity = 498/585 (85.13%), Postives = 521/585 (89.06%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESS S FPGTYLGLV+KLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYNSVLGDYK+NFWGYSTVG+ SPMIRYSSAGI+NCGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDN+VYYMLAPM               
Sbjct: 346 KEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWVIEMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVIEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDP+LQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHW VWSEWNGKYRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYFRWDK+ EESSS FHRFC 
Sbjct: 646 RNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKM-EESSSDFHRFCR 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFRYECESLGLNDFPTA+RLQWHGHSPG P+WS ASRFVAF+LVDSVK EIYIAFNA
Sbjct: 706 LMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGY+WEPLVDTGKISPFDFL+SDL ERDVAI+Q
Sbjct: 766 SHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQ 779

[Close]

BLAST of Sgr027021 vs. NCBI nr
Match: KAG6587707.1 (Isoamylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1042.0 bits (2693), Expect = 2.8e-300
Identity = 497/585 (84.96%), Postives = 525/585 (89.74%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESS S FPGTYLGLV+KLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYNSVLGDYK+NFWGYSTVG+ SPMIRYSSAGI+NCGQDAINEFK LI
Sbjct: 286 MPCHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDN+VYYMLAP+++        TL   
Sbjct: 346 KEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPILL--------TLDGC 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
             +   +F             H  +  FI          +   RYWVIEMHVDGFRFDLA
Sbjct: 406 FKFKLGEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVIEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDP+LQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHW VWSEWNGKYRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYFRWDK+ EESSS FHRFC 
Sbjct: 646 RNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKM-EESSSDFHRFCR 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFRYECESLGLNDFPTA+RLQWHGHSPG P+WS ASRFVAF+LVDSVK EIYIAFNA
Sbjct: 706 LMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGY+WEPLVDTGKISPFDFL+SDL +RDVAI+Q
Sbjct: 766 SHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEKRDVAIQQ 791

[Close]

BLAST of Sgr027021 vs. NCBI nr
Match: XP_038879251.1 (isoamylase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1040.0 bits (2688), Expect = 1.1e-299
Identity = 497/585 (84.96%), Postives = 518/585 (88.55%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQH QKDLIIYEMHVRGFTRHESS S+FPGTYLGLVEKLDYLKELGVNCIEL
Sbjct: 229 FDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIEL 288

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEYFSYNSVLGDYKMNFWGYSTV YFSPMIRYSSA I NCGQDAINEFKLLI
Sbjct: 289 MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSASIGNCGQDAINEFKLLI 348

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           REAHKRGIEVIMDVVFNHTAEGNENGP  SFRGVDN+VYYMLAPM               
Sbjct: 349 REAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPM--------------- 408

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWV EMHVDGFRFDLA
Sbjct: 409 -----GEFYNYSGCGNTFNCNHPAVRTFI----------VDCLRYWVTEMHVDGFRFDLA 468

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNV+GNPIEGD LTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG
Sbjct: 469 SIMTRGSSLWDAVNVFGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 528

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHWGVWSEWNG+YRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 529 LYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 588

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 589 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 648

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVP+I MGDEYGHTKGGNNNTYCHDNY+NYFRWDK+ EESSS FHRFCS
Sbjct: 649 RNFFVCLMVSQGVPLILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKM-EESSSDFHRFCS 708

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFRYECESLGLNDFPT ERLQWHGHSPGKP+W+ ASRFVAF+LVDSVK E+YIAFNA
Sbjct: 709 LMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNA 768

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGYRW+PLVDTGKISPFDFL+SDLPERDVAI+Q
Sbjct: 769 SHLPVTVTLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQ 782

[Close]

BLAST of Sgr027021 vs. NCBI nr
Match: KAG7021674.1 (Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1038.9 bits (2685), Expect = 2.4e-299
Identity = 496/585 (84.79%), Postives = 520/585 (88.89%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESS S FPGTYLGLV+KLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYNSVLGDYK+NFWGYSTVG+ SPMIRYSSAGI+NCGQDAINEFK LI
Sbjct: 286 MPCHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKFLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDN+VYYMLAPM               
Sbjct: 346 KEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWVIEMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVIEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDP+LQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHW VWSEWNGKYRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYFRWDK+ EESSS FHRFC 
Sbjct: 646 RNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKM-EESSSDFHRFCR 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFRYECESLGLNDFPTA+RLQWHGHSPG P+WS ASRFVAF+LVDSVK EIYIAFNA
Sbjct: 706 LMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGY+WEPLVDTGKISPFDFL+SDL +RDVAI+Q
Sbjct: 766 SHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEKRDVAIQQ 779

[Close]

BLAST of Sgr027021 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 894.8 bits (2311), Expect = 7.3e-259
Identity = 417/583 (71.53%), Postives = 470/583 (80.62%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDW+GDLPL++PQKDL+IYEMH+RGFT+H SS+ + PGTY+G + KLDYLKELGVNC+EL
Sbjct: 230 FDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVEHPGTYIGAISKLDYLKELGVNCVEL 289

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEYFS +S     KMNFWGYST+ +FSPMIRYSS GI+NCG+DAINEFK  +
Sbjct: 290 MPCHEFNELEYFSCSS-----KMNFWGYSTINFFSPMIRYSSGGIRNCGRDAINEFKTFV 349

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           REAHKRGIEVIMDVVFNHTAEGNE GPI SFRG+DNS YYMLAP                
Sbjct: 350 REAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGIDNSTYYMLAPK--------------- 409

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H ++  FI          +   RYWV EMHVDGFRFDLA
Sbjct: 410 -----GEFYNYSGCGNTFNCNHPVVREFI----------VDCLRYWVTEMHVDGFRFDLA 469

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRG SLWD VNVYG+P+EGD  TTG+PL  PPLIDMISNDP+L  VKLIAEAWDAGG
Sbjct: 470 SIMTRGCSLWDPVNVYGSPVEGDMTTTGTPLATPPLIDMISNDPILGDVKLIAEAWDAGG 529

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVG FPHW +WSEWNGKYRDIVRQFIKGT+GF+G FAECLCGSP+LYQ GGRKPW+SI
Sbjct: 530 LYQVGQFPHWKIWSEWNGKYRDIVRQFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSI 589

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NF+CAHDGFTLADLVTYN K+N +NGEDN+DGE+HN SWNCG+EGEF  +SVK+LRKRQM
Sbjct: 590 NFVCAHDGFTLADLVTYNKKYNSSNGEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQM 649

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFV LMVSQGVPM +MGDEYGHTKGGNNNTYCHD+Y+NYFRWDK EE  SS   RFCS
Sbjct: 650 RNFFVSLMVSQGVPMFYMGDEYGHTKGGNNNTYCHDHYVNYFRWDKKEE--SSDLQRFCS 709

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMT FR +CESLGL DFPTA+RL WHGH PGKP+WS  SRFVAFS  D  KGEIY+AFNA
Sbjct: 710 LMTKFRKQCESLGLADFPTAQRLHWHGHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNA 769

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAI 584
           +HLP  V LPERPGYRWEPLVDTGK +P+DFL+ DLP+R  A+
Sbjct: 770 SHLPAVVGLPERPGYRWEPLVDTGKPAPYDFLTDDLPDRAHAV 775

[Close]

BLAST of Sgr027021 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 892.9 bits (2306), Expect = 2.8e-258
Identity = 420/585 (71.79%), Postives = 479/585 (81.88%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGD+ L+ PQKDL+IYEMHVRGFTRHESS  +FPGTY G+ EKLD+LKELG+NCIEL
Sbjct: 203 FDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIEL 262

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYN++LGD+++NFWGYST+G+FSPMIRY+SA   N    AINEFK+L+
Sbjct: 263 MPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILV 322

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVV NHTAEGNE GPIFSFRGVDNSVYYMLAP                
Sbjct: 323 KEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPK--------------- 382

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H ++  FI        +D    RYWV EMHVDGFRFDL 
Sbjct: 383 -----GEFYNYSGCGNTFNCNHPVVRQFI--------LDCL--RYWVTEMHVDGFRFDLG 442

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIM+R SSLWDA NVYG  +EGD LTTG+P+  PP+IDMISNDP+L+GVKLIAEAWDAGG
Sbjct: 443 SIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGG 502

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVG FPHWG+WSEWNGK+RD+VRQFIKGT+GFSGAFAECLCGSPNLYQ GGRKPW+SI
Sbjct: 503 LYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSI 562

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYN+K+NLANGE+N DGE+HN SWNCG+EG+F SISVK+LRKRQM
Sbjct: 563 NFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQM 622

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFV LMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNY+NYFRWDK EE  S +F RFC 
Sbjct: 623 RNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFF-RFCR 682

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           ++  FR ECESLGLNDFPTA+RLQWHG +P  PNWS  SRFVAFSLVDSVK EIY+AFN 
Sbjct: 683 ILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNT 742

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HL   V+LP RPGYRWEP VDT K SP+D ++ DLPER+ A++Q
Sbjct: 743 SHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQ 755

[Close]

BLAST of Sgr027021 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 470.7 bits (1210), Expect = 3.4e-131
Identity = 260/581 (44.75%), Postives = 349/581 (60.07%), Query Frame = 0


Query: 1   FDWEGDLPLQH-PQKDLIIYEMHVRGFTRHESS--HSKFPGTYLGLVEKLDYLKELGVNC 60
           FDW  D    + P+KDL+IYEM+VR FT  ESS       G+YLG +EK+ +L++LG+N 
Sbjct: 220 FDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINA 279

Query: 61  IELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFK 120
           +EL+P  EF+ELE     S   D+ +N WGYSTV +F+PM RY+S   +     A  EFK
Sbjct: 280 VELLPVFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG--EGDPIKASKEFK 339

Query: 121 LLIREAHKRGIEVIMDVVFNHTAEGNENGP-IFSFRGVDNSVYYMLAPMIIASCWFSSST 180
            +++  H  GIEVI+DVV+NHT E ++  P   SFRG+DN VYYML P         ++ 
Sbjct: 340 EMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP---------NNQ 399

Query: 181 LLALPLWSFPQFLPPHARSCDGKL--MHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDG 240
           LL             +   C   L   H +++          E+ +   R+WV E HVDG
Sbjct: 400 LL-------------NFSGCGNTLNCNHPVVM----------ELILDSLRHWVTEYHVDG 459

Query: 241 FRFDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAE 300
           FRFDLAS++ R                    T GSPL  PPLI  I+ D VL   K+IAE
Sbjct: 460 FRFDLASVLCRA-------------------TDGSPLSAPPLIRAIAKDSVLSRCKIIAE 519

Query: 301 AWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGR 360
            WD GGLY VG FP+W  W+EWNG YRD VR+FIKG +G  G+FA  + GS +LYQ   R
Sbjct: 520 PWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQR 579

Query: 361 KPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKK 420
           KP++ +NF+ AHDGFTL DLV+YN KHN ANGE   DG + N+SWNCG EGE     +K 
Sbjct: 580 KPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKS 639

Query: 421 LRKRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSY 480
           LR RQM+NF + LM+SQG PM+ MGDEYGHT+ GNNN+Y HD  +N F+W +++ +  ++
Sbjct: 640 LRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNH 699

Query: 481 FHRFCSLMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNW-SAASRFVAFSLVDSVKG- 540
           F RF S +  FR+    L   +F T   + WH     + NW ++ S+F+AF+L D + G 
Sbjct: 700 F-RFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNSESKFLAFTLHDGIGGR 740

Query: 541 EIYIAFNANHLPVTVTLPE-RPGYRWEPLVDTGKISPFDFL 573
           +IY+AFNA+   V   +P+  PG +W  + DT   SP DF+
Sbjct: 760 DIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV 740

[Close]

BLAST of Sgr027021 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 463.4 bits (1191), Expect = 5.4e-129
Identity = 261/584 (44.69%), Postives = 348/584 (59.59%), Query Frame = 0


Query: 1   FDWEGDLPLQH-PQKDLIIYEMHVRGFTRHESS--HSKFPGTYLGLVEKLDYLKELGVNC 60
           FDW  D  L + P+ DL+IYEM+VR FT  ESS   S   G+YLGL++K+ +L ELGVN 
Sbjct: 235 FDWGDDYRLPNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNA 294

Query: 61  IELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFK 120
           +EL+P  E++ELE+  Y +   D+ +N WGYST+ +F+PM RY+SAG       A  E K
Sbjct: 295 VELLPVFEYDELEFKRYPNP-RDHMVNTWGYSTINFFAPMSRYASAG--GGPVAASKELK 354

Query: 121 LLIREAHKRGIEVIMDVVFNHTAEGNENGP-IFSFRGVDNSVYYMLAPMIIASCWFSSST 180
            +++E HK GIEVI+DVV+NHT E ++  P + SFRG+DN VYYML     A     S  
Sbjct: 355 QMVKELHKAGIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGC 414

Query: 181 LLALPLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFR 240
                          +  +C+  ++  +IL  +              R+WV E H+DGFR
Sbjct: 415 --------------GNTLNCNHPVVKELILDSL--------------RHWVEEYHIDGFR 474

Query: 241 FDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAW 300
           FDLAS++ RG                     G PL  PPLI  I+ D VL   K+IAE W
Sbjct: 475 FDLASVLCRGPD-------------------GCPLDAPPLIKEIAKDAVLSRCKIIAEPW 534

Query: 301 DAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKP 360
           D GGLY VG FP+W  W+EWNGKYRD +R+FIKG  G  G FA  + GS +LYQ   RKP
Sbjct: 535 DCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNERKP 594

Query: 361 WNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLR 420
           ++ +NF+ AHDGFTL DLV+YN KHN ANGE   DG + N SWNCG EGE   ++V  LR
Sbjct: 595 YHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVLSLR 654

Query: 421 KRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFH 480
            RQM+NF V LM+SQG PM+ MGDEYGHT+ GNNN+Y HD  IN F+W+++E+    +F 
Sbjct: 655 SRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDGHF- 714

Query: 481 RFCSLMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNW-SAASRFVAFSLVD-SVKGEI 540
           RF S M  FR+    L  + F     + WH     +  W +  S+F+AF++ D +  G+I
Sbjct: 715 RFFSEMIKFRHSNPILRRDRFLNKNDVTWH-----EDCWENQESKFLAFTVHDHNSGGDI 762

Query: 541 YIAFNANHLPVTVTLPERPGYR-WEPLVDTGKISPFDFLSSDLP 578
           Y+AFNA+   V   +P  P ++ W  +VDT   SP D +   +P
Sbjct: 775 YLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVP 762

[Close]

BLAST of Sgr027021 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 374.4 bits (960), Expect = 3.3e-102
Identity = 210/476 (44.12%), Postives = 275/476 (57.77%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTR-HESSHSKFPGTYLGLVEK--LDYLKELGVNC 60
           FDW  D   + P  + +IYE HV+G T+ H S   +  GTY GL     +D+L EL V  
Sbjct: 158 FDWAYDRSPRTPYHETVIYEAHVKGMTQTHPSIPPELRGTYAGLAHPVIIDHLNELNVTA 217

Query: 61  IELMPCHEFNELEYFSYNSVLGDYKM-NFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEF 120
           +ELMP H+      F ++S L D  + N+WGY+T G+F+P  +Y+S   +  G  A+ EF
Sbjct: 218 VELMPVHQ------FLHDSRLLDLGLRNYWGYNTFGFFAPHHQYAST--RQAG-SAVAEF 277

Query: 121 KLLIREAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSST 180
           K ++R  H+ GIEVI+DVV+NHTAEGN  GP  +FRG+DN+ YY L    +         
Sbjct: 278 KTMVRSLHEAGIEVILDVVYNHTAEGNHLGPTINFRGIDNTAYYRLMDHDL--------- 337

Query: 181 LLALPLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFR 240
                   F +       S + +  H + LI          MD    RYWVIEMHVDGFR
Sbjct: 338 -------RFYKDFTGTGNSLNARHPHTLQLI----------MD--SLRYWVIEMHVDGFR 397

Query: 241 FDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAW 300
           FDLAS + R     D ++ +                     D++  DPV+  VKLIAE W
Sbjct: 398 FDLASTLARELHDVDRLSAF--------------------FDLVQQDPVVSQVKLIAEPW 457

Query: 301 DAG-GLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRK 360
           D G G YQVG FP  G+W+EWNGKYRD VR + +G     G FA  L GS +LY+  GR+
Sbjct: 458 DVGEGGYQVGNFP--GLWTEWNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRR 517

Query: 361 PWNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKL 420
           P  SINF+ AHDGFTL DLV+YN KHN ANGE+N+DGES+N SWNCG EG      +  L
Sbjct: 518 PSASINFVTAHDGFTLNDLVSYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILAL 574

Query: 421 RKRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEES 472
           R RQM+N +  LMVSQG PMI  GDE G T+ GNNN YC D+ +++  W  +++ +
Sbjct: 578 RARQMRNMWATLMVSQGTPMIAHGDEIGRTQYGNNNVYCQDSELSWMDWSLVDKNA 574

[Close]

BLAST of Sgr027021 vs. ExPASy TrEMBL
Match: A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)

HSP 1 Score: 1059.3 bits (2738), Expect = 8.3e-306
Identity = 508/585 (86.84%), Postives = 526/585 (89.91%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQ+PQKDLIIYEMHVRGFTRHESSHS+FPGTYLGLVEKLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQYPQKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVG+FSPMIRYSSAGI+ CGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPM               
Sbjct: 346 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWV EMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVTEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHWGVWSEWNG+YRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYN+KHNLANGEDN DGESHNNSWNCGQEGEFVS+SVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNYINYFRWDK+ EESSS FHRFCS
Sbjct: 646 RNFFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKL-EESSSDFHRFCS 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           L+TTFR+ECESLGLNDFPTAERLQWHG+SPGKP+WS ASRFVAF+LVDSVKGEIYIAFNA
Sbjct: 706 LVTTFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ
Sbjct: 766 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 779

[Close]

BLAST of Sgr027021 vs. ExPASy TrEMBL
Match: A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)

HSP 1 Score: 1042.0 bits (2693), Expect = 1.4e-300
Identity = 498/585 (85.13%), Postives = 521/585 (89.06%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESS S FPGTYLGLV+KLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYNSVLGDYK+NFWGYSTVG+ SPMIRYSSAGI+NCGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDN+VYYMLAPM               
Sbjct: 346 KEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWVIEMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVIEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDP+LQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHW VWSEWNGKYRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYFRWDK+ EESSS FHRFC 
Sbjct: 646 RNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKM-EESSSDFHRFCR 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFRYECESLGLNDFPTA+RLQWHGHSPG P+WS ASRFVAF+LVDSVK EIYIAFNA
Sbjct: 706 LMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGY+WEPLVDTGKISPFDFL+SDL ERDVAI+Q
Sbjct: 766 SHLPVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQ 779

[Close]

BLAST of Sgr027021 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 1033.9 bits (2672), Expect = 3.7e-298
Identity = 496/585 (84.79%), Postives = 518/585 (88.55%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESS S  PGTYLGLV+KLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYNSVLGDYK+NFWGYSTVG+ SPMIRYSSAGI+NCGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYYSYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDN+VYYMLAPM               
Sbjct: 346 KEAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWVIEMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRRFI----------VDCLRYWVIEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDP+LQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISNDPILQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHW VWSEWNGKYRDIVRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKPWNSI
Sbjct: 526 LYQVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNYINYF WDK+ EESSS FHRFC 
Sbjct: 646 RNFFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWDKM-EESSSDFHRFCR 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFRYECESLGLNDFPTA+RLQWHGHSPG P+WS ASRFVAF+LVDSVK EIYIAFNA
Sbjct: 706 LMTTFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPER GYRWEPLVDTGKISPFDFL+SDL ERDVAI+Q
Sbjct: 766 SHLPVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQ 779

[Close]

BLAST of Sgr027021 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 1.2e-296
Identity = 491/585 (83.93%), Postives = 516/585 (88.21%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQH QKDLIIYEMHVRGFTRHESS S FPGTYLGLVEKLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEYFSYNSVLGDYKMNFWGYSTV YFSPMIRYSSAGI NCGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           REAHKRGIEVIMDVVFNHTAEGNENGP  SFRGVDN+VYYMLAPM               
Sbjct: 346 REAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWV EMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRTFI----------VDCLRYWVTEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHWGVWSEWNGKYRD+VRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKP NSI
Sbjct: 526 LYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NF+CAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NYFRWDK+ EES+S FHRFCS
Sbjct: 646 RNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKM-EESTSDFHRFCS 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFR+ECESLGLNDFPT ERLQWHGHSPGKP+W+ ASRFVAF+LVDSVK E+YIAFNA
Sbjct: 706 LMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGYRW+PLVDTGK SPFDFL++D+PER++AI+Q
Sbjct: 766 SHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQ 779

[Close]

BLAST of Sgr027021 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 1028.9 bits (2659), Expect = 1.2e-296
Identity = 491/585 (83.93%), Postives = 516/585 (88.21%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGDLPLQH QKDLIIYEMHVRGFTRHESS S FPGTYLGLVEKLDYLKELGVNCIEL
Sbjct: 226 FDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIEL 285

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEYFSYNSVLGDYKMNFWGYSTV YFSPMIRYSSAGI NCGQDAINEFKLLI
Sbjct: 286 MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLI 345

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           REAHKRGIEVIMDVVFNHTAEGNENGP  SFRGVDN+VYYMLAPM               
Sbjct: 346 REAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPM--------------- 405

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H  +  FI          +   RYWV EMHVDGFRFDLA
Sbjct: 406 -----GEFYNYSGCGNTFNCNHPAVRTFI----------VDCLRYWVTEMHVDGFRFDLA 465

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG
Sbjct: 466 SIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 525

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVGTFPHWGVWSEWNGKYRD+VRQFIKGT+GFSGAFAECLCGSPNLYQGGGRKP NSI
Sbjct: 526 LYQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSI 585

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NF+CAHDGFTLADLVTYNSKHNLANGEDN DGE+HNNSWNCGQEGEFVSISVKKLRKRQM
Sbjct: 586 NFVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQM 645

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFVCLMVSQGVPMI MGDEYGHTKGGNNNTYCHDNY+NYFRWDK+ EES+S FHRFCS
Sbjct: 646 RNFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKM-EESTSDFHRFCS 705

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           LMTTFR+ECESLGLNDFPT ERLQWHGHSPGKP+W+ ASRFVAF+LVDSVK E+YIAFNA
Sbjct: 706 LMTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNA 765

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HLPVTVTLPERPGYRW+PLVDTGK SPFDFL++D+PER++AI+Q
Sbjct: 766 SHLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQ 779

[Close]

BLAST of Sgr027021 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 892.9 bits (2306), Expect = 2.0e-259
Identity = 420/585 (71.79%), Postives = 479/585 (81.88%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSHSKFPGTYLGLVEKLDYLKELGVNCIEL 60
           FDWEGD+ L+ PQKDL+IYEMHVRGFTRHESS  +FPGTY G+ EKLD+LKELG+NCIEL
Sbjct: 203 FDWEGDMHLKLPQKDLVIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIEL 262

Query: 61  MPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFKLLI 120
           MPCHEFNELEY+SYN++LGD+++NFWGYST+G+FSPMIRY+SA   N    AINEFK+L+
Sbjct: 263 MPCHEFNELEYYSYNTILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILV 322

Query: 121 REAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSSTLLAL 180
           +EAHKRGIEVIMDVV NHTAEGNE GPIFSFRGVDNSVYYMLAP                
Sbjct: 323 KEAHKRGIEVIMDVVLNHTAEGNEKGPIFSFRGVDNSVYYMLAPK--------------- 382

Query: 181 PLWSFPQFLPPHARSCDGKLMHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDGFRFDLA 240
                 +F             H ++  FI        +D    RYWV EMHVDGFRFDL 
Sbjct: 383 -----GEFYNYSGCGNTFNCNHPVVRQFI--------LDCL--RYWVTEMHVDGFRFDLG 442

Query: 241 SIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEAWDAGG 300
           SIM+R SSLWDA NVYG  +EGD LTTG+P+  PP+IDMISNDP+L+GVKLIAEAWDAGG
Sbjct: 443 SIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLIAEAWDAGG 502

Query: 301 LYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGRKPWNSI 360
           LYQVG FPHWG+WSEWNGK+RD+VRQFIKGT+GFSGAFAECLCGSPNLYQ GGRKPW+SI
Sbjct: 503 LYQVGMFPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSI 562

Query: 361 NFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKKLRKRQM 420
           NFICAHDGFTLADLVTYN+K+NLANGE+N DGE+HN SWNCG+EG+F SISVK+LRKRQM
Sbjct: 563 NFICAHDGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQM 622

Query: 421 QNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSYFHRFCS 480
           +NFFV LMVSQGVPMI+MGDEYGHTKGGNNNTYCHDNY+NYFRWDK EE  S +F RFC 
Sbjct: 623 RNFFVSLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFF-RFCR 682

Query: 481 LMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNWSAASRFVAFSLVDSVKGEIYIAFNA 540
           ++  FR ECESLGLNDFPTA+RLQWHG +P  PNWS  SRFVAFSLVDSVK EIY+AFN 
Sbjct: 683 ILIKFRDECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNT 742

Query: 541 NHLPVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQ 586
           +HL   V+LP RPGYRWEP VDT K SP+D ++ DLPER+ A++Q
Sbjct: 743 SHLATLVSLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQ 755

[Close]

BLAST of Sgr027021 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 470.7 bits (1210), Expect = 2.4e-132
Identity = 260/581 (44.75%), Postives = 349/581 (60.07%), Query Frame = 0


Query: 1   FDWEGDLPLQH-PQKDLIIYEMHVRGFTRHESS--HSKFPGTYLGLVEKLDYLKELGVNC 60
           FDW  D    + P+KDL+IYEM+VR FT  ESS       G+YLG +EK+ +L++LG+N 
Sbjct: 220 FDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIPHLQDLGINA 279

Query: 61  IELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEFK 120
           +EL+P  EF+ELE     S   D+ +N WGYSTV +F+PM RY+S   +     A  EFK
Sbjct: 280 VELLPVFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYASG--EGDPIKASKEFK 339

Query: 121 LLIREAHKRGIEVIMDVVFNHTAEGNENGP-IFSFRGVDNSVYYMLAPMIIASCWFSSST 180
            +++  H  GIEVI+DVV+NHT E ++  P   SFRG+DN VYYML P         ++ 
Sbjct: 340 EMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDP---------NNQ 399

Query: 181 LLALPLWSFPQFLPPHARSCDGKL--MHLIILIFIRTIYLVFEMDIFGYRYWVIEMHVDG 240
           LL             +   C   L   H +++          E+ +   R+WV E HVDG
Sbjct: 400 LL-------------NFSGCGNTLNCNHPVVM----------ELILDSLRHWVTEYHVDG 459

Query: 241 FRFDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAE 300
           FRFDLAS++ R                    T GSPL  PPLI  I+ D VL   K+IAE
Sbjct: 460 FRFDLASVLCRA-------------------TDGSPLSAPPLIRAIAKDSVLSRCKIIAE 519

Query: 301 AWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGR 360
            WD GGLY VG FP+W  W+EWNG YRD VR+FIKG +G  G+FA  + GS +LYQ   R
Sbjct: 520 PWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGSFATRVSGSSDLYQVNQR 579

Query: 361 KPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKK 420
           KP++ +NF+ AHDGFTL DLV+YN KHN ANGE   DG + N+SWNCG EGE     +K 
Sbjct: 580 KPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKS 639

Query: 421 LRKRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSY 480
           LR RQM+NF + LM+SQG PM+ MGDEYGHT+ GNNN+Y HD  +N F+W +++ +  ++
Sbjct: 640 LRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTSLNNFQWKELDAKKQNH 699

Query: 481 FHRFCSLMTTFRYECESLGLNDFPTAERLQWHGHSPGKPNW-SAASRFVAFSLVDSVKG- 540
           F RF S +  FR+    L   +F T   + WH     + NW ++ S+F+AF+L D + G 
Sbjct: 700 F-RFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNSESKFLAFTLHDGIGGR 740

Query: 541 EIYIAFNANHLPVTVTLPE-RPGYRWEPLVDTGKISPFDFL 573
           +IY+AFNA+   V   +P+  PG +W  + DT   SP DF+
Sbjct: 760 DIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV 740

[Close]

BLAST of Sgr027021 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 241.9 bits (616), Expect = 1.8e-63
Identity = 178/587 (30.32%), Postives = 268/587 (45.66%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSH--SKFPGTYLGLVEKLDYLKELGVNCI 60
           FDW  D+    P + L++Y ++V+GFT+H SS   S   GT+ G+ EK+ +LK LG N +
Sbjct: 349 FDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAV 408

Query: 61  ELMPCHEFNELE--YFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEF 120
            L P   F+E +  YF ++                 +FSPM  Y   G  N  + A+N  
Sbjct: 409 LLEPIFSFSEQKGPYFPFH-----------------FFSPMDIY---GPSNSLESAVNSM 468

Query: 121 KLLIREAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSST 180
           K+++++ H  GIEV+++VVF HTA+        + RG+D+S YY                
Sbjct: 469 KVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDSSYYYKG------------- 528

Query: 181 LLALPLWSFPQFLPPHARSCDGKLMHLIILIFIRTIY-LVFEMDIFGYRYWVIEMHVDGF 240
                           A   D K        ++   Y +V ++ +   RYWV E HVDGF
Sbjct: 529 ---------------RANDLDSK-------SYLNCNYPVVQQLVLESLRYWVTEFHVDGF 588

Query: 241 RFDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEA 300
            F  AS + RG                     G  L  PPL++ I+ DP+L   KLIA+ 
Sbjct: 589 CFINASSLLRG-------------------VHGEQLSRPPLVEAIAFDPLLAETKLIADC 648

Query: 301 WDAGGLY-QVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGR 360
           WD   +  +   FPHW  W+E N +Y   VR F++G  G     A  +CGS +++   GR
Sbjct: 649 WDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRG-RGVLSDLATRICGSGDVFT-DGR 708

Query: 361 KPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKK 420
            P  S N+I  + G +L D+V++ S   LA+            SWNCG+EG     +V +
Sbjct: 709 GPAFSFNYISRNSGLSLVDIVSF-SGPELAS----------ELSWNCGEEGATNKSAVLQ 768

Query: 421 LRKRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSY 480
            R +Q++NF     +S GVP++ MGDE G +  G+            F W+ +     + 
Sbjct: 769 RRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLASAFGTQ 828

Query: 481 FHRFCSLMTTFR-YECESLGLNDFPTAERLQWHGHSPGKPNW-SAASRFVAFSLVDSVKG 540
             +F S MT+ R    +     DF   E + W+ +    P W   AS+F+A  +    + 
Sbjct: 829 ITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEE 838

Query: 541 E---------------IYIAFNANHLPVTVTLPERP-GYRWEPLVDT 564
           E               ++I FNA+  P +V LP  P G +W  LVDT
Sbjct: 889 EETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDT 838

[Close]

BLAST of Sgr027021 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 241.9 bits (616), Expect = 1.8e-63
Identity = 178/587 (30.32%), Postives = 268/587 (45.66%), Query Frame = 0


Query: 1   FDWEGDLPLQHPQKDLIIYEMHVRGFTRHESSH--SKFPGTYLGLVEKLDYLKELGVNCI 60
           FDW  D+    P + L++Y ++V+GFT+H SS   S   GT+ G+ EK+ +LK LG N +
Sbjct: 349 FDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGTFSGVAEKVSHLKTLGTNAV 408

Query: 61  ELMPCHEFNELE--YFSYNSVLGDYKMNFWGYSTVGYFSPMIRYSSAGIQNCGQDAINEF 120
            L P   F+E +  YF ++                 +FSPM  Y   G  N  + A+N  
Sbjct: 409 LLEPIFSFSEQKGPYFPFH-----------------FFSPMDIY---GPSNSLESAVNSM 468

Query: 121 KLLIREAHKRGIEVIMDVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMIIASCWFSSST 180
           K+++++ H  GIEV+++VVF HTA+        + RG+D+S YY                
Sbjct: 469 KVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDSSYYYKG------------- 528

Query: 181 LLALPLWSFPQFLPPHARSCDGKLMHLIILIFIRTIY-LVFEMDIFGYRYWVIEMHVDGF 240
                           A   D K        ++   Y +V ++ +   RYWV E HVDGF
Sbjct: 529 ---------------RANDLDSK-------SYLNCNYPVVQQLVLESLRYWVTEFHVDGF 588

Query: 241 RFDLASIMTRGSSLWDAVNVYGNPIEGDALTTGSPLGNPPLIDMISNDPVLQGVKLIAEA 300
            F  AS + RG                     G  L  PPL++ I+ DP+L   KLIA+ 
Sbjct: 589 CFINASSLLRG-------------------VHGEQLSRPPLVEAIAFDPLLAETKLIADC 648

Query: 301 WDAGGLY-QVGTFPHWGVWSEWNGKYRDIVRQFIKGTNGFSGAFAECLCGSPNLYQGGGR 360
           WD   +  +   FPHW  W+E N +Y   VR F++G  G     A  +CGS +++   GR
Sbjct: 649 WDPLEMMPKEVRFPHWKRWAELNTRYCRNVRNFLRG-RGVLSDLATRICGSGDVFT-DGR 708

Query: 361 KPWNSINFICAHDGFTLADLVTYNSKHNLANGEDNKDGESHNNSWNCGQEGEFVSISVKK 420
            P  S N+I  + G +L D+V++ S   LA+            SWNCG+EG     +V +
Sbjct: 709 GPAFSFNYISRNSGLSLVDIVSF-SGPELAS----------ELSWNCGEEGATNKSAVLQ 768

Query: 421 LRKRQMQNFFVCLMVSQGVPMIFMGDEYGHTKGGNNNTYCHDNYINYFRWDKIEEESSSY 480
            R +Q++NF     +S GVP++ MGDE G +  G+            F W+ +     + 
Sbjct: 769 RRLKQIRNFLFIQYISLGVPVLNMGDECGISTRGSPLLESR----KPFDWNLLASAFGTQ 828

Query: 481 FHRFCSLMTTFR-YECESLGLNDFPTAERLQWHGHSPGKPNW-SAASRFVAFSLVDSVKG 540
             +F S MT+ R    +     DF   E + W+ +    P W   AS+F+A  +    + 
Sbjct: 829 ITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQTTPKWEDPASKFLALEIKSESEE 838

Query: 541 E---------------IYIAFNANHLPVTVTLPERP-GYRWEPLVDT 564
           E               ++I FNA+  P +V LP  P G +W  LVDT
Sbjct: 889 EETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPDGSKWRRLVDT 838

[Close]

BLAST of Sgr027021 vs. TAIR 10
Match: AT5G35320.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 48.1 bits (113), Expect = 3.9e-05
Identity = 39/116 (33.62%), Postives = 56/116 (48.28%), Query Frame = 0


Query: 730 DDDEMGTE--NKNLAVVPWIPRLQVPAPTNMHVPLEEAPQLMEAEDVGE------ATMDI 789
           DD++   E  NK  AVV W P     + +          ++ E ++ GE      A+ +I
Sbjct: 110 DDNQYEDERLNKCQAVVCWNPSQSTYSQSIGTFQQPRTLEITELDETGEDVVMDDASNEI 169

Query: 790 EDDSLSSHQPYGYGGMDG------GNAVHQWHQ-QHCMIPQLPQ--QTSTPISWFR 829
           E+D+ S+   +   G         G  +H W Q Q+CMIPQLPQ   T TPI+WFR
Sbjct: 170 EEDTGSTSLSFPQQGQQQEQEHTYGFGLHPWQQAQNCMIPQLPQVSTTPTPITWFR 225

The following BLAST results are available for this feature:

BLAST of Sgr027021 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022134724.1	1.7e-305	86.84	isoamylase 1, chloroplastic [Momordica charantia]	[more]
XP_022932124.1	2.8e-300	85.13	isoamylase 1, chloroplastic [Cucurbita moschata]	[more]
KAG6587707.1	2.8e-300	84.96	Isoamylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038879251.1	1.1e-299	84.96	isoamylase 1, chloroplastic [Benincasa hispida]	[more]
KAG7021674.1	2.4e-299	84.79	Isoamylase 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of Sgr027021 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D0TZF0	7.3e-259	71.53	Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=...	[more]
O04196	2.8e-258	71.79	Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1	[more]
Q9M0S5	3.4e-131	44.75	Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2	[more]
B9G434	5.4e-129	44.69	Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=...	[more]
P0A4Y5	3.3e-102	44.12	Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935...	[more]

BLAST of Sgr027021 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BZK5	8.3e-306	86.84	isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 ...	[more]
A0A6J1EW54	1.4e-300	85.13	isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 S...	[more]
A0A6J1ICQ3	3.7e-298	84.79	isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=...	[more]
A0A5A7UQM5	1.2e-296	83.93	Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 ...	[more]
A0A1S3BVW8	1.2e-296	83.93	isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1	[more]

BLAST of Sgr027021 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G39930.1	2.0e-259	71.79	isoamylase 1	[more]
AT4G09020.1	2.4e-132	44.75	isoamylase 3	[more]
AT1G03310.2	1.8e-63	30.32	debranching enzyme 1	[more]
AT1G03310.1	1.8e-63	30.32	debranching enzyme 1	[more]
AT5G35320.1	3.9e-05	33.62	unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0...	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	SMART	SM00642	aamy	coord: 20..486 e-value: 1.6E-6 score: -20.4
IPR006047	Glycosyl hydrolase, family 13, catalytic domain	PFAM	PF00128	Alpha-amylase	coord: 38..144 e-value: 9.9E-13 score: 48.3
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 216..500 e-value: 6.1E-111 score: 373.5 coord: 1..182 e-value: 7.2E-68 score: 231.4
None	No IPR available	PANTHER	PTHR43002	GLYCOGEN DEBRANCHING ENZYME	coord: 1..165
None	No IPR available	PANTHER	PTHR43002	GLYCOGEN DEBRANCHING ENZYME	coord: 224..590
None	No IPR available	PANTHER	PTHR43002:SF1	ISOAMYLASE 1, CHLOROPLASTIC	coord: 1..165
None	No IPR available	PANTHER	PTHR43002:SF1	ISOAMYLASE 1, CHLOROPLASTIC	coord: 224..590
None	No IPR available	CDD	cd11326	AmyAc_Glg_debranch	coord: 1..486 e-value: 0.0 score: 661.471
None	No IPR available	SUPERFAMILY	51011	Glycosyl hydrolase domain	coord: 502..576
IPR013780	Glycosyl hydrolase, all-beta	GENE3D	2.60.40.1180		coord: 502..593 e-value: 1.7E-25 score: 91.3
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 1..491

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr027021.1	Sgr027021.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005977	glycogen metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0019156	isoamylase activity
molecular_function	GO:0003824	catalytic activity