Sgr026469 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr026469 vs. NCBI nr
Match: XP_038883594.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 347.8 bits (891), Expect = 9.0e-92
Identity = 191/301 (63.46%), Postives = 215/301 (71.43%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSA-AGNY-------RHRHHHFRP 60
           M+++L +LFS   F+ S                SGQGSA AG Y        HR H  RP
Sbjct: 1   MNFNLSLLFSFFFFMIS--------------LFSGQGSASAGRYYGDHHHHHHRRHRLRP 60

Query: 61  TKFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLS 120
           TK FVFGDSYVDTGNI+ PFSSA+QFPYGITFPGKP+GRFSDG VLTD L          
Sbjct: 61  TKLFVFGDSYVDTGNILLPFSSAQQFPYGITFPGKPSGRFSDGRVLTDFL---------- 120

Query: 121 KIQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPL 180
                           A+H+G+KSPIPF++R   G +RLK+ GINFAFGGTG FDTSVPL
Sbjct: 121 ----------------AKHLGVKSPIPFSIRSEVGVERLKESGINFAFGGTGVFDTSVPL 180

Query: 181 PNMTTQIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVV 240
           PNMTTQIDFFQ+LR  +SVF+N DA+ S+AL SVSGNDYSF+L T+ S +GLKPFINSVV
Sbjct: 181 PNMTTQIDFFQHLRHVESVFSNGDAQHSVALVSVSGNDYSFYLATNGSAQGLKPFINSVV 240

Query: 241 NQIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 292
           NQIVIDLKRI  LGVKKIVVTGLGPLGCLP FTAP SF+QCN TINS V FHNFLL QAV
Sbjct: 241 NQIVIDLKRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVRFHNFLLNQAV 261

BLAST of Sgr026469 vs. NCBI nr
Match: KAG6604360.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 342.0 bits (876), Expect = 4.9e-90
Identity = 180/296 (60.81%), Postives = 206/296 (69.59%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRHHH----FRPTKFF 60
           MD+ LR+LFSLL+ +S                 SG+GSA G Y H HHH    FRPTK F
Sbjct: 2   MDFVLRLLFSLLLLIS---------------LFSGRGSAGGVYNHHHHHLRHRFRPTKLF 61

Query: 61  VFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQK 120
           VFGDSY DTGNI+FP S A QFPYGITFPGKP GRFSDG VLTD                
Sbjct: 62  VFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDF--------------- 121

Query: 121 EALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLPNMT 180
                       ARHVGLKSPIP+++R   G +R K+ G+NFAFGGTG FDT  PLPNMT
Sbjct: 122 -----------AARHVGLKSPIPYSVRSRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMT 181

Query: 181 TQIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIV 240
           TQIDFFQ LR  DS+FTN D + S+AL S SGNDYSF+   + S +GLKPFI SVVNQI+
Sbjct: 182 TQIDFFQQLRHGDSIFTNTDVQSSVALVSASGNDYSFYFAMNSSRQGLKPFIMSVVNQII 241

Query: 241 IDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           ++LKRI  LGVKKI VTGLGPLGCLP FTAPSS+++CN TINSLV+FHNFLLK+AV
Sbjct: 242 VNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKKCNETINSLVQFHNFLLKEAV 256

BLAST of Sgr026469 vs. NCBI nr
Match: KAA0025271.1 (GDSL esterase/lipase [Cucumis melo var. makuwa])

HSP 1 Score: 340.5 bits (872), Expect = 1.4e-89
Identity = 183/295 (62.03%), Postives = 210/295 (71.19%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRH---HHFRPTKFFV 60
           MD++L++LF+   F  S                SGQGSA G Y +RH   H  R TK FV
Sbjct: 1   MDFNLKLLFTFFFFTVS--------------LFSGQGSAGGYYSNRHHRSHRLRATKLFV 60

Query: 61  FGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQKE 120
           FGDSYVDTGNI+ PFSSA+QFPYGITFPGKP+GRFSDG VLTD                 
Sbjct: 61  FGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDF---------------- 120

Query: 121 ALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLPNMTT 180
                       +H+G+KSPIPF++R   G +RLK+ GINFAFGGTG FDTSVPLPNMTT
Sbjct: 121 ----------AGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTT 180

Query: 181 QIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIVI 240
           QID F+ LR A+S  +NRD  LS+AL SVSGNDYSF+L T+ S +GLKPFINSVVNQ++I
Sbjct: 181 QIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMI 240

Query: 241 DLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           DLKRI  LGVKKIVVTGLGPLGCLP FTAP SF+QCN TINS V+FHNFLLKQAV
Sbjct: 241 DLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAV 255

BLAST of Sgr026469 vs. NCBI nr
Match: XP_023544846.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 337.8 bits (865), Expect = 9.3e-89
Identity = 178/299 (59.53%), Postives = 205/299 (68.56%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRH-------HHFRPT 60
           MD+ LR+LFSLL+ +S                 SG+GSA G Y H H       H FRPT
Sbjct: 1   MDFVLRLLFSLLLLIS---------------LFSGRGSAGGVYNHHHHRRRHLRHRFRPT 60

Query: 61  KFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSK 120
           K FVFGDSY DTGNI+FP S A QFPYGITFPGKP GRFSDG VLTD             
Sbjct: 61  KLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDF------------ 120

Query: 121 IQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLP 180
                          ARHVGLKSPIPF++R   G +RLK+ G+NFAFGGTG FDT  P P
Sbjct: 121 --------------AARHVGLKSPIPFSVRNRVGLRRLKETGVNFAFGGTGVFDTEYPFP 180

Query: 181 NMTTQIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVN 240
           NMTTQIDFFQ LR  DS+FTN+DA+ S+AL S SGNDYSF+   + + +GLKPFI SVVN
Sbjct: 181 NMTTQIDFFQQLRHGDSIFTNKDAQSSVALVSASGNDYSFYFAMNSTRQGLKPFIMSVVN 240

Query: 241 QIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           QI+++LKRI  LGVKKI VTGLGPLGCLP FTAPSS++QCN T+NSLV+FHN LLK+ V
Sbjct: 241 QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCNETVNSLVQFHNLLLKEGV 258

BLAST of Sgr026469 vs. NCBI nr
Match: XP_004143379.2 (GDSL esterase/lipase At5g03610 [Cucumis sativus] >KGN48533.2 hypothetical protein Csa_003217 [Cucumis sativus])

HSP 1 Score: 337.0 bits (863), Expect = 1.6e-88
Identity = 183/298 (61.41%), Postives = 209/298 (70.13%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNY-----RHRHHHFRPTKF 60
           MD++L++LF+   F  S                SGQGSA G Y     RHR H  R TK 
Sbjct: 1   MDFNLKLLFTFFFFTLS--------------LFSGQGSAGGFYGNLRHRHRSHPSRGTKL 60

Query: 61  FVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQ 120
           FVFGDSYVDTGNI+FPFSSA QFPYGITFPGKP+GRFSDG VLTD               
Sbjct: 61  FVFGDSYVDTGNILFPFSSAEQFPYGITFPGKPSGRFSDGRVLTDF-------------- 120

Query: 121 KEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLPNM 180
                        A+H+G+KSPIPF++R   G +RLK+ GINFAFGGTG F+T VPLPNM
Sbjct: 121 ------------AAKHLGVKSPIPFSIRSEVGEERLKESGINFAFGGTGVFNTLVPLPNM 180

Query: 181 TTQIDFFQNLRAD--SVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQ 240
           TTQID F+ LR D   + +NRD  LSLAL SVSGNDYSF+L T+ S +GLKPFINSVVNQ
Sbjct: 181 TTQIDLFEQLRDDESGLISNRDVHLSLALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQ 240

Query: 241 IVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           I++DL+RI  LGVKKIVVTGLGPLGCLP FTAP SF+QCN TINS V+FHNFLLKQAV
Sbjct: 241 IMVDLRRIRRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAV 258

BLAST of Sgr026469 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 246.9 bits (629), Expect = 2.8e-64
Identity = 140/292 (47.95%), Postives = 172/292 (58.90%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRHHHFRPTKFFVFGD 60
           MD  +++ F L IFL + L    +            G    N  H  + FRPTK FVFGD
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEI-----------NGVEGSNQNHHLYPFRPTKLFVFGD 60

Query: 61  SYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQKEALQ 120
           SY DTGNI   FSS+ +FPYGITFPGKP GRFSDG V TD L                  
Sbjct: 61  SYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFL------------------ 120

Query: 121 NYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDTSVPLPNMTTQIDF 180
                   A+ VG+KSPIP+  +  AG KRL+ G+NFA+GGTG F+T  PLPNMTTQID 
Sbjct: 121 --------AKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDI 180

Query: 181 FQN-LRADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIVIDLKR 240
           FQN L    ++   +   S+AL SV+GNDYS F+  +        FI  VV+Q  ++L+R
Sbjct: 181 FQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRR 240

Query: 241 ISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAVA 292
           I +LGVKKI V  L PLGCLP FT  +SFQ+CN T N+LV  HN LL+Q VA
Sbjct: 241 IHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVA 255

BLAST of Sgr026469 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 1.4e-55
Identity = 117/243 (48.15%), Postives = 150/243 (61.73%), Query Frame = 0

Query: 51  RPTKFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLS 110
           RP + FVFGDSY DTGNI    S + + PYGITFP KP+GRFSDG V TD L        
Sbjct: 36  RPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFL-------- 95

Query: 111 LSKIQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDT-SV 170
                             AR++G+KSPIP+  +  AG +RL  G+N+A+GGTG F T   
Sbjct: 96  ------------------ARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDN 155

Query: 171 PLPNMTTQIDFFQN-LRADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINS 230
           PLPNMTTQID+FQ  L A ++++  D   SLAL SV+GNDY+ FL     +  L  F+  
Sbjct: 156 PLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQ 215

Query: 231 VVNQIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQ 290
           VV+QI ++  RI  LGV KIV+  + PLGCLP  T  +SFQ+CNAT N+    HN+LL +
Sbjct: 216 VVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHK 252

Query: 291 AVA 292
           A+A
Sbjct: 276 AIA 252

BLAST of Sgr026469 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 160.6 bits (405), Expect = 2.7e-38
Identity = 107/262 (40.84%), Postives = 142/262 (54.20%), Query Frame = 0

Query: 35  GQGSAAGNYRHRHHHFRPTKFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSD 94
           G   A G+ +    +  P K FVFG+SY DTGN + P + + + PYGITFPGKP+GR+SD
Sbjct: 16  GINGAVGSNQPSQANKNP-KLFVFGNSYADTGN-MKPTALSWKLPYGITFPGKPSGRYSD 75

Query: 95  GLVLTDLLVNNSLSLSLSKIQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLK-- 154
           GL  TD L                          A+ +G K P    L +  G K++K  
Sbjct: 76  GLTATDFL--------------------------AKQLGAKLPY---LWRTHGKKKVKLN 135

Query: 155 QGINFAFGGTGAFDTSVP-LPNMTTQIDFFQNLR-ADSVFT-NRDARLSLALASVSGNDY 214
           +G+NFAFGG+  FD+ V   PN++TQ+ F  NL  A  V+T + D   S AL S SG DY
Sbjct: 136 RGMNFAFGGSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDY 195

Query: 215 SFFLDTHDSMEGLKPFINSVVNQIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQ 274
             F+D + +M     F+  +V  I   L  ++ L  K I VT L PLGCLPR T  SSF+
Sbjct: 196 YGFIDQNPNMAAYPAFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFR 246

Query: 275 QCNATINSLVEFHNFLLKQAVA 292
            CN + + LV  HN  LK+AVA
Sbjct: 256 SCNESYSDLVRLHNESLKKAVA 246

BLAST of Sgr026469 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 159.5 bits (402), Expect = 5.9e-38
Identity = 107/286 (37.41%), Postives = 150/286 (52.45%), Query Frame = 0

Query: 8   LFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRHHHFRPTKFFVFGDSYVDTGN 67
           LFS LI LS  +      +V   +S S + +    +      F+PTK FVFGDSY DTGN
Sbjct: 11  LFSCLILLSDYI------KVNSSISPSSEQTQEDGF----FGFKPTKLFVFGDSYADTGN 70

Query: 68  IIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQKEALQNYYNSRG 127
             F    + +FP GITFPG PTGRFSDG V TD L                         
Sbjct: 71  TPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYL------------------------- 130

Query: 128 QARHVGLKSPIPFNLRQLAGFKRL--KQGINFAFGGTGAFDTSVPL-PNMTTQIDFFQNL 187
            A+++G+++PI +   +  G  RL  K+G+NFA+GG GAF+T   L P  + QID F+ L
Sbjct: 131 -AKYIGVRTPITYKWGKY-GRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQL 190

Query: 188 RADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIVIDLKRISSLG 247
              +V++  D   S+A  S+ GNDY  +   + S EG       VV QI++D+KRI  LG
Sbjct: 191 LMRNVYSPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLG 250

Query: 248 VKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           V+K++V    P  CLP+   P     C+ T ++    HN LL++ +
Sbjct: 251 VRKVLVALSPPQKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGL 255

BLAST of Sgr026469 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 2.3e-37
Identity = 93/240 (38.75%), Postives = 131/240 (54.58%), Query Frame = 0

Query: 54  KFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSK 113
           K FVFGDSY DTGN     + A   PYGITFPGKP+GR+ DGL+ TD L           
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFL----------- 73

Query: 114 IQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDTS--VPL 173
                           + +G +S  P+  R     K LK+G+NFAFGG+   D+S   P 
Sbjct: 74  ---------------EKVLGAES--PYLYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPF 133

Query: 174 PNMTTQIDFFQNLRADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVN 233
           PN+T Q++F  +L              ++L S +G DY +++D +    GLK  +  VV+
Sbjct: 134 PNITAQVNFLVDLVLAGRVYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVD 193

Query: 234 QIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAVA 292
            + +++  +  L  KKI VT L P+GCLP +T+ SSF+ CN + ++LVE HN LLK+ VA
Sbjct: 194 NLRVNMIVLGGLLFKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVA 224

BLAST of Sgr026469 vs. ExPASy TrEMBL
Match: A0A5A7SLZ4 (GDSL esterase/lipase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold541G001230 PE=3 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 6.9e-90
Identity = 183/295 (62.03%), Postives = 210/295 (71.19%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRH---HHFRPTKFFV 60
           MD++L++LF+   F  S                SGQGSA G Y +RH   H  R TK FV
Sbjct: 1   MDFNLKLLFTFFFFTVS--------------LFSGQGSAGGYYSNRHHRSHRLRATKLFV 60

Query: 61  FGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQKE 120
           FGDSYVDTGNI+ PFSSA+QFPYGITFPGKP+GRFSDG VLTD                 
Sbjct: 61  FGDSYVDTGNILVPFSSAQQFPYGITFPGKPSGRFSDGRVLTDF---------------- 120

Query: 121 ALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLPNMTT 180
                       +H+G+KSPIPF++R   G +RLK+ GINFAFGGTG FDTSVPLPNMTT
Sbjct: 121 ----------AGKHLGVKSPIPFSIRNEVGEERLKESGINFAFGGTGVFDTSVPLPNMTT 180

Query: 181 QIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIVI 240
           QID F+ LR A+S  +NRD  LS+AL SVSGNDYSF+L T+ S +GLKPFINSVVNQ++I
Sbjct: 181 QIDLFEQLRHAESGLSNRDVHLSVALVSVSGNDYSFYLATNGSAQGLKPFINSVVNQVMI 240

Query: 241 DLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           DLKRI  LGVKKIVVTGLGPLGCLP FTAP SF+QCN TINS V+FHNFLLKQAV
Sbjct: 241 DLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSFKQCNQTINSFVQFHNFLLKQAV 255

BLAST of Sgr026469 vs. ExPASy TrEMBL
Match: A0A6J1ECT1 (GDSL esterase/lipase At5g03610-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.0e-88
Identity = 179/299 (59.87%), Postives = 205/299 (68.56%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRH-------HHFRPT 60
           MD+ LR+LFSLL+ +S                 SG+GSA G Y H H       H FRPT
Sbjct: 1   MDFVLRLLFSLLLLIS---------------LFSGRGSAGGVYNHHHHRRRHLRHRFRPT 60

Query: 61  KFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSK 120
           K FVFGDSY DTGNI+FP S A QFPYGITFPGKP GRFSDG VLTD             
Sbjct: 61  KLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDF------------ 120

Query: 121 IQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLP 180
                          ARHVGLKSPIPF++R   G +R K+ G+NFAFGGTG FDT  PLP
Sbjct: 121 --------------AARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLP 180

Query: 181 NMTTQIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVN 240
           NMTTQIDFFQ LR  DS+FTN DA+ S+AL S SGNDY+F+   + S +GLKPFI SVV+
Sbjct: 181 NMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVS 240

Query: 241 QIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           QI+++LKRI  LGVKKI VTGLGPLGCLP FTAPSS++QCN TINSLV+FHN LLK+AV
Sbjct: 241 QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAV 258

BLAST of Sgr026469 vs. ExPASy TrEMBL
Match: A0A6J1EIS2 (GDSL esterase/lipase At5g03610-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111433004 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 1.0e-88
Identity = 179/299 (59.87%), Postives = 205/299 (68.56%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRH-------HHFRPT 60
           MD+ LR+LFSLL+ +S                 SG+GSA G Y H H       H FRPT
Sbjct: 2   MDFVLRLLFSLLLLIS---------------LFSGRGSAGGVYNHHHHRRRHLRHRFRPT 61

Query: 61  KFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSK 120
           K FVFGDSY DTGNI+FP S A QFPYGITFPGKP GRFSDG VLTD             
Sbjct: 62  KLFVFGDSYADTGNILFPISPALQFPYGITFPGKPIGRFSDGRVLTDF------------ 121

Query: 121 IQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLP 180
                          ARHVGLKSPIPF++R   G +R K+ G+NFAFGGTG FDT  PLP
Sbjct: 122 --------------AARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLP 181

Query: 181 NMTTQIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVN 240
           NMTTQIDFFQ LR  DS+FTN DA+ S+AL S SGNDY+F+   + S +GLKPFI SVV+
Sbjct: 182 NMTTQIDFFQQLRHGDSIFTNTDAQSSVALVSASGNDYAFYFAMNSSRQGLKPFIMSVVS 241

Query: 241 QIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           QI+++LKRI  LGVKKI VTGLGPLGCLP FTAPSS++QCN TINSLV+FHN LLK+AV
Sbjct: 242 QIIVNLKRIGGLGVKKIAVTGLGPLGCLPVFTAPSSYKQCNETINSLVQFHNLLLKEAV 259

BLAST of Sgr026469 vs. ExPASy TrEMBL
Match: A0A1S3CJ29 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500972 PE=3 SV=1)

HSP 1 Score: 335.9 bits (860), Expect = 1.7e-88
Identity = 175/262 (66.79%), Postives = 198/262 (75.57%), Query Frame = 0

Query: 34  SGQGSAAGNYRHRH---HHFRPTKFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTG 93
           +GQGSA G Y +RH   H  R TK FVFGDSYVDTGNI+ PFSSA+QFPYGITFPGKP+G
Sbjct: 2   TGQGSAGGYYSNRHHRSHRLRATKLFVFGDSYVDTGNILVPFSSAQQFPYGITFPGKPSG 61

Query: 94  RFSDGLVLTDLLVNNSLSLSLSKIQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKR 153
           RFSDG VLTD                             +H+G+KSPIPF++R   G +R
Sbjct: 62  RFSDGRVLTDF--------------------------AGKHLGVKSPIPFSIRNEVGEER 121

Query: 154 LKQ-GINFAFGGTGAFDTSVPLPNMTTQIDFFQNLR-ADSVFTNRDARLSLALASVSGND 213
           LK+ GINFAFGGTG FDTSVPLPNMTTQID F+ LR A+S  +NRD  LS+AL SVSGND
Sbjct: 122 LKESGINFAFGGTGVFDTSVPLPNMTTQIDLFEQLRHAESGLSNRDVHLSVALVSVSGND 181

Query: 214 YSFFLDTHDSMEGLKPFINSVVNQIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSF 273
           YSF+L T+ S +GLKPFINSVVNQ++IDLKRI  LGVKKIVVTGLGPLGCLP FTAP SF
Sbjct: 182 YSFYLATNGSAQGLKPFINSVVNQVMIDLKRIKRLGVKKIVVTGLGPLGCLPIFTAPFSF 237

Query: 274 QQCNATINSLVEFHNFLLKQAV 291
           +QCN TINS V+FHNFLLKQAV
Sbjct: 242 KQCNQTINSFVQFHNFLLKQAV 237

BLAST of Sgr026469 vs. ExPASy TrEMBL
Match: A0A6J1IRZ9 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111477899 PE=3 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 6.5e-88
Identity = 177/296 (59.80%), Postives = 203/296 (68.58%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRH----HHFRPTKFF 60
           MD+ LR+LFSLL+ +S                 SG+GSA G Y H H    H FRPTK F
Sbjct: 2   MDFVLRLLFSLLLLIS---------------LFSGRGSAGGVYNHHHQQLRHRFRPTKLF 61

Query: 61  VFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQK 120
           VFGDSY DTGNI+FP S   QFPYGITFPGKP GRFSDG VLTD                
Sbjct: 62  VFGDSYADTGNILFPISPGMQFPYGITFPGKPIGRFSDGRVLTDF--------------- 121

Query: 121 EALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQ-GINFAFGGTGAFDTSVPLPNMT 180
                       ARHVGLKSPIPF++R   G +R K+ G+NFAFGGTG FDT  PLPNMT
Sbjct: 122 -----------AARHVGLKSPIPFSVRNRVGLRRFKETGVNFAFGGTGVFDTEYPLPNMT 181

Query: 181 TQIDFFQNLR-ADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIV 240
           TQIDFFQ+LR  DS+ TN DA+ S+AL S SGNDYSF+   + S +GLKPFI SVVNQI+
Sbjct: 182 TQIDFFQHLRQGDSILTNTDAQSSVALVSASGNDYSFYFAMNTSRQGLKPFIISVVNQIM 241

Query: 241 IDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           ++LKRI  LGVKKI VTGLGPLGC P FTAPSS++QCN +INSLV+FHNFLLK+ V
Sbjct: 242 VNLKRIGGLGVKKIAVTGLGPLGCFPVFTAPSSYKQCNESINSLVQFHNFLLKEGV 256

BLAST of Sgr026469 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.0e-65
Identity = 140/292 (47.95%), Postives = 172/292 (58.90%), Query Frame = 0

Query: 1   MDYSLRILFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRHHHFRPTKFFVFGD 60
           MD  +++ F L IFL + L    +            G    N  H  + FRPTK FVFGD
Sbjct: 1   MDSLIKLFFCLFIFLCTSLLFGEI-----------NGVEGSNQNHHLYPFRPTKLFVFGD 60

Query: 61  SYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQKEALQ 120
           SY DTGNI   FSS+ +FPYGITFPGKP GRFSDG V TD L                  
Sbjct: 61  SYADTGNIKKAFSSSWKFPYGITFPGKPAGRFSDGRVATDFL------------------ 120

Query: 121 NYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDTSVPLPNMTTQIDF 180
                   A+ VG+KSPIP+  +  AG KRL+ G+NFA+GGTG F+T  PLPNMTTQID 
Sbjct: 121 --------AKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYGGTGVFNTQTPLPNMTTQIDI 180

Query: 181 FQN-LRADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIVIDLKR 240
           FQN L    ++   +   S+AL SV+GNDYS F+  +        FI  VV+Q  ++L+R
Sbjct: 181 FQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPASEFPAFIKQVVDQTEVNLRR 240

Query: 241 ISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAVA 292
           I +LGVKKI V  L PLGCLP FT  +SFQ+CN T N+LV  HN LL+Q VA
Sbjct: 241 IHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALVNLHNNLLQQVVA 255

BLAST of Sgr026469 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-56
Identity = 117/243 (48.15%), Postives = 150/243 (61.73%), Query Frame = 0

Query: 51  RPTKFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLS 110
           RP + FVFGDSY DTGNI    S + + PYGITFP KP+GRFSDG V TD L        
Sbjct: 36  RPNRLFVFGDSYADTGNIRKSLSDSWKIPYGITFPQKPSGRFSDGRVATDFL-------- 95

Query: 111 LSKIQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDT-SV 170
                             AR++G+KSPIP+  +  AG +RL  G+N+A+GGTG F T   
Sbjct: 96  ------------------ARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGGTGVFKTKDN 155

Query: 171 PLPNMTTQIDFFQN-LRADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINS 230
           PLPNMTTQID+FQ  L A ++++  D   SLAL SV+GNDY+ FL     +  L  F+  
Sbjct: 156 PLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPLTELPAFMKQ 215

Query: 231 VVNQIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQ 290
           VV+QI ++  RI  LGV KIV+  + PLGCLP  T  +SFQ+CNAT N+    HN+LL +
Sbjct: 216 VVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNASTNLHNYLLHK 252

Query: 291 AVA 292
           A+A
Sbjct: 276 AIA 252

BLAST of Sgr026469 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 4.2e-39
Identity = 107/286 (37.41%), Postives = 150/286 (52.45%), Query Frame = 0

Query: 8   LFSLLIFLSSQLNESWVTEVGKYVSISGQGSAAGNYRHRHHHFRPTKFFVFGDSYVDTGN 67
           LFS LI LS  +      +V   +S S + +    +      F+PTK FVFGDSY DTGN
Sbjct: 11  LFSCLILLSDYI------KVNSSISPSSEQTQEDGF----FGFKPTKLFVFGDSYADTGN 70

Query: 68  IIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSKIQKEALQNYYNSRG 127
             F    + +FP GITFPG PTGRFSDG V TD L                         
Sbjct: 71  TPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYL------------------------- 130

Query: 128 QARHVGLKSPIPFNLRQLAGFKRL--KQGINFAFGGTGAFDTSVPL-PNMTTQIDFFQNL 187
            A+++G+++PI +   +  G  RL  K+G+NFA+GG GAF+T   L P  + QID F+ L
Sbjct: 131 -AKYIGVRTPITYKWGKY-GRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQL 190

Query: 188 RADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVNQIVIDLKRISSLG 247
              +V++  D   S+A  S+ GNDY  +   + S EG       VV QI++D+KRI  LG
Sbjct: 191 LMRNVYSPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLG 250

Query: 248 VKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAV 291
           V+K++V    P  CLP+   P     C+ T ++    HN LL++ +
Sbjct: 251 VRKVLVALSPPQKCLPKLVTPKG---CD-TNDTSTYLHNSLLRKGL 255

BLAST of Sgr026469 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 157.5 bits (397), Expect = 1.6e-38
Identity = 93/240 (38.75%), Postives = 131/240 (54.58%), Query Frame = 0

Query: 54  KFFVFGDSYVDTGNIIFPFSSARQFPYGITFPGKPTGRFSDGLVLTDLLVNNSLSLSLSK 113
           K FVFGDSY DTGN     + A   PYGITFPGKP+GR+ DGL+ TD L           
Sbjct: 14  KLFVFGDSYADTGNTKRD-TEAWAIPYGITFPGKPSGRYCDGLIATDFL----------- 73

Query: 114 IQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDTS--VPL 173
                           + +G +S  P+  R     K LK+G+NFAFGG+   D+S   P 
Sbjct: 74  ---------------EKVLGAES--PYLYRTHGRDKGLKRGMNFAFGGSKMLDSSPNSPF 133

Query: 174 PNMTTQIDFFQNLRADSVFTNRDARLSLALASVSGNDYSFFLDTHDSMEGLKPFINSVVN 233
           PN+T Q++F  +L              ++L S +G DY +++D +    GLK  +  VV+
Sbjct: 134 PNITAQVNFLVDLVLAGRVYGDITPSDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVD 193

Query: 234 QIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPSSFQQCNATINSLVEFHNFLLKQAVA 292
            + +++  +  L  KKI VT L P+GCLP +T+ SSF+ CN + ++LVE HN LLK+ VA
Sbjct: 194 NLRVNMIVLGGLLFKKIAVTSLQPIGCLPSYTSASSFKSCNESQSALVELHNKLLKKVVA 224

BLAST of Sgr026469 vs. TAIR 10
Match: AT5G18430.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 99.4 bits (246), Expect = 5.2e-21
Identity = 81/256 (31.64%), Postives = 126/256 (49.22%), Query Frame = 0

Query: 55  FFVFGDSYVDTGNIIFPFSSAR--QFPYGITFP-GKPTGRFSDGLVLTDLLVNNSLSLSL 114
           FFVFGDS VD+GN  +  ++AR    PYGI FP  +PTGRFS+GL + DL+         
Sbjct: 29  FFVFGDSLVDSGNNNYLVTTARADSPPYGIDFPTRRPTGRFSNGLNIPDLI--------- 88

Query: 115 SKIQKEALQNYYNSRGQARHVGLKSPIPFNLRQLAGFKRLKQGINFAFGGTGAFDTS--- 174
                EA+ N             + P+P+   +L G + L  G NFA  G G  + +   
Sbjct: 89  ----SEAIGNE------------EPPLPYLSPELRG-RSLLNGANFASAGIGILNDTGFQ 148

Query: 175 -VPLPNMTTQIDFFQNLR------ADSVFTNRDARLSLALASVSGNDY--SFFLDTHDSM 234
            + +  M  Q+D+FQ  +           T R    +L L +V GND+  ++FL  + + 
Sbjct: 149 FINIIRMYQQLDYFQQYQQRVSRLIGKPQTQRLVSQALVLITVGGNDFVNNYFLFPYSAR 208

Query: 235 E---GLKPFINSVVNQIVIDLKRISSLGVKKIVVTGLGPLGCLPRFTAPS--SFQQCNAT 291
                L  ++  ++++    L R++SLGV +++VTG GPLGC P   A S  S  +C+A 
Sbjct: 209 SRQFTLPDYVRLLISEYKKILLRLNSLGVGRVLVTGAGPLGCAPAELARSGTSNGRCSAE 258