Sgr026468 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr026468 vs. NCBI nr
Match: XP_022133081.1 (GDSL esterase/lipase At5g03610-like [Momordica charantia])

HSP 1 Score: 596.3 bits (1536), Expect = 1.7e-166
Identity = 289/351 (82.34%), Postives = 313/351 (89.17%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSAR-HHRHFHFRPTRLFVFGDSYVDTGNINSTASSS 60
           MD +KLLF LIFSL +FSLL GQGSAR HHRHFH RPTRLFVFGDSYVDTGNIN   SS+
Sbjct: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFGG 120
           RN PYGITFPGVPSGR SDGRVLTDYLA YF LKSP+PF+ WQNVG KRP +GINFAFGG
Sbjct: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG 120

Query: 121 TGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVEG 180
           TG+FDTSFPFPNMTTQI+FFQKLI NSIFT NDIHSS+ALVS SGNDYSFY ATNGS+EG
Sbjct: 121 TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG 180

Query: 181 FKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVEF 240
           FKPFI+SVVNQIA+NLKRIH LGV+KVV+ GLGP+GCLP+LTA SSF +CNATM+SLVEF
Sbjct: 181 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 240

Query: 241 HNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGS-VRGRARFETPLTPCCFGVSS 300
           HN LLKQAVEKLN ETKGSPFFILD+Y TLLSIIQNKGS  + R RFETPL PCCFGVSS
Sbjct: 241 HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKHL 350
           EFSCGSVD++GN+KYTLCKDPK ALFWDSVHPT EGWF AF SL S+LK L
Sbjct: 301 EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351

BLAST of Sgr026468 vs. NCBI nr
Match: XP_038883507.1 (GDSL esterase/lipase At5g03610-like [Benincasa hispida])

HSP 1 Score: 547.0 bits (1408), Expect = 1.2e-151
Identity = 270/351 (76.92%), Postives = 298/351 (84.90%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASSSR 60
           M  +KLLF  + SL +FSLL+GQGSA    HFH  PT+L VFGDSYVDTGNIN   S +R
Sbjct: 2   MASKKLLFTFL-SLLIFSLLSGQGSA---GHFHIHPTKLIVFGDSYVDTGNINVNTSGAR 61

Query: 61  NFPYGITFPGVPSGRFSDGRVLTDYLASYFRL-KSPLPFSVWQNVGTKRPIHGINFAFGG 120
           NFPYGITFPGVPSGRFSDGRVLTD+ A Y  L KSP PF  WQ +G KRP  GINFAFGG
Sbjct: 62  NFPYGITFPGVPSGRFSDGRVLTDFFAKYLGLKKSPTPFIAWQKIGNKRPKFGINFAFGG 121

Query: 121 TGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVEG 180
           TGVFDTSFPFPNMTTQI+ FQ LI NSIFT+ DIHSS+ALVSPSGNDYSFYLATNGS +G
Sbjct: 122 TGVFDTSFPFPNMTTQINLFQNLIANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSPQG 181

Query: 181 FKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVEF 240
           FKPF+ISVVNQIAVNLKRI+ LGVKK+VV GLGPVGC PQLTAP SF +CNATMNS VEF
Sbjct: 182 FKPFVISVVNQIAVNLKRIYRLGVKKIVVVGLGPVGCYPQLTAP-SFNKCNATMNSFVEF 241

Query: 241 HNLLLKQAVEKLNSETKGSP-FFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVSS 300
           HN+LLK+AVEKLN+ETKGSP FFILD+YDT+LSIIQNKG+ +    F+TPL PCCFGVSS
Sbjct: 242 HNILLKKAVEKLNNETKGSPNFFILDMYDTVLSIIQNKGNPKVGVSFKTPLKPCCFGVSS 301

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKHL 350
           EFSCGSVDE GN+ YTLCK P  ALFWDS+HPT +GWFAAFSSLRS+LKHL
Sbjct: 302 EFSCGSVDEHGNKMYTLCKHPNLALFWDSLHPTQKGWFAAFSSLRSNLKHL 347

BLAST of Sgr026468 vs. NCBI nr
Match: XP_023518578.1 (GDSL esterase/lipase At5g03610-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.4 bits (1391), Expect = 1.1e-149
Identity = 262/350 (74.86%), Postives = 294/350 (84.00%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHF--RPTRLFVFGDSYVDTGNINSTASS 60
           M+ RKL+FPL+ SLFVFSLL+GQGS R H  + F  RPT+LFVFGDSYVDTGNI ++ SS
Sbjct: 1   MESRKLVFPLL-SLFVFSLLSGQGSGRRHVDYRFNSRPTKLFVFGDSYVDTGNIKASTSS 60

Query: 61  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 120
           SRNFPYGITFPGVPSGRFSDGRVLTD+ A+Y  LKSP  FS W+  G K P +G+NFAFG
Sbjct: 61  SRNFPYGITFPGVPSGRFSDGRVLTDFFANYLELKSPETFSGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVE 180
           GTGVFDTSFPFPNMTTQIDFFQ L+ N+IF    + SS+ALVSPSGNDYSFY   NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFAPKHVGSSVALVSPSGNDYSFYQERNGSIE 180

Query: 181 GFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVE 240
           G +PFIISVVNQIAVNLKRIHSLGVKK+VV GLGPVGCLPQ TA SSFK CNAT NS+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIHSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNATSNSMAK 240

Query: 241 FHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVSS 300
            HNLLLKQAVEKLN ETKGSPF I D+Y+ +LSII+NKG+ + R RFETPL PCCFGVSS
Sbjct: 241 VHNLLLKQAVEKLNKETKGSPFIIFDVYNAILSIIRNKGNPKARVRFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKH 349
           EFSCGSVDE+GN+ YTLC  PKS LFWDSVHPT +GWFA FSSL S LK+
Sbjct: 301 EFSCGSVDEKGNKMYTLCTRPKSTLFWDSVHPTQQGWFATFSSLASSLKY 349

BLAST of Sgr026468 vs. NCBI nr
Match: XP_022962769.1 (GDSL esterase/lipase At5g03610-like [Cucurbita moschata])

HSP 1 Score: 540.0 bits (1390), Expect = 1.5e-149
Identity = 264/350 (75.43%), Postives = 293/350 (83.71%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHF--RPTRLFVFGDSYVDTGNINSTASS 60
           M+ RKL+FPL+ SLFVFSLL GQGS R H  + F  RPT+LFVFGDSYVDTGNI +T SS
Sbjct: 1   MESRKLVFPLL-SLFVFSLLLGQGSGRRHVDYRFNSRPTKLFVFGDSYVDTGNIKATTSS 60

Query: 61  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 120
           SRNFPYGITFPGVPSGRFSDGRVLTD+ A Y  LKSP  FS W+  G K P +G+NFAFG
Sbjct: 61  SRNFPYGITFPGVPSGRFSDGRVLTDFFADYLELKSPETFSGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVE 180
           GTGVFDTSFPFPNMTTQIDFFQ L+ N+IF    + SS+ALVSPSGNDYSFY   NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFAPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVE 240
           G +PFIISVVNQIAVNLKRIHSLGVKK+VV GLG VGCLPQ TA SSFK CNAT NS+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIHSLGVKKIVVLGLGAVGCLPQPTANSSFKHCNATSNSMAK 240

Query: 241 FHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVSS 300
            HNLLLKQAVEKLN ETKGSPF I D+Y+ +LSII+NKG+ + RARFETPL PCCFGVSS
Sbjct: 241 VHNLLLKQAVEKLNKETKGSPFIIFDVYNAILSIIRNKGNPKARARFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKH 349
           EFSCGSVDE+G + YTLCK PKSALFWDSVHPT +GWFA FSSL S LK+
Sbjct: 301 EFSCGSVDEKGKKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLKY 349

BLAST of Sgr026468 vs. NCBI nr
Match: XP_022972691.1 (GDSL esterase/lipase At5g03610-like [Cucurbita maxima])

HSP 1 Score: 532.3 bits (1370), Expect = 3.1e-147
Identity = 259/350 (74.00%), Postives = 291/350 (83.14%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHFR--PTRLFVFGDSYVDTGNINSTASS 60
           M+ RK +FPL+ SLFVFSLL G+GS RHH  + F   PT+LFVFGDSYVDTGNI +T SS
Sbjct: 1   MESRKTVFPLL-SLFVFSLLLGRGSGRHHVDYRFNSWPTKLFVFGDSYVDTGNIKATNSS 60

Query: 61  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 120
           SRN PYGITFPGVPSGRFSDGRV+TD+ A+Y  LKSP  F  W+  G K P +G+NFAFG
Sbjct: 61  SRNLPYGITFPGVPSGRFSDGRVMTDFFANYLELKSPETFIGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVE 180
           GTGVFDTSFPFPNMTTQIDFFQ L+ N+IF    + SS+ALVSPSGNDYSFY   NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFDPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVE 240
           G +PFIISVVNQIAVNLKRI SLGVKK+VV GLGPVGCLPQ TA SSFK CN+T NS+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIQSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNSTTNSMAK 240

Query: 241 FHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVSS 300
            HNLLLKQAVEKLN +TKGSPF I D+Y+ +LSII+NKGS + RARF TPL PCCFGVSS
Sbjct: 241 VHNLLLKQAVEKLNKDTKGSPFIIFDVYNAILSIIRNKGSPKARARFVTPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKH 349
           EFSCGSVDE+GN+ YTLCK PKSALFWDSVHPT +GWFA FSSL S LKH
Sbjct: 301 EFSCGSVDEKGNKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLKH 349

BLAST of Sgr026468 vs. ExPASy Swiss-Prot
Match: Q9LZS7 (GDSL esterase/lipase At5g03610 OS=Arabidopsis thaliana OX=3702 GN=At5g03610 PE=2 SV=1)

HSP 1 Score: 394.0 bits (1011), Expect = 1.7e-108
Identity = 195/349 (55.87%), Postives = 246/349 (70.49%), Query Frame = 0

Query: 7   LFPLIFSLFVF---SLLAG-----QGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASS 66
           L  L F LF+F   SLL G     +GS ++H  + FRPT+LFVFGDSY DTGNI    SS
Sbjct: 4   LIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKKAFSS 63

Query: 67  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 126
           S  FPYGITFPG P+GRFSDGRV TD+LA +  +KSP+P+      G KR  +G+NFA+G
Sbjct: 64  SWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYG 123

Query: 127 GTGVFDTSFPFPNMTTQIDFFQKLI-TNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSV 186
           GTGVF+T  P PNMTTQID FQ ++ T  I+   ++ SS+ALVS +GNDYS ++A N   
Sbjct: 124 GTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPA 183

Query: 187 EGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLV 246
             F  FI  VV+Q  VNL+RIH+LGVKK+ V  L P+GCLP  T  +SF++CN T N+LV
Sbjct: 184 SEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALV 243

Query: 247 EFHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVS 306
             HN LL+Q V KLN+ETK S F ILDLY+  L++ +NKGS  G  RFE+PL PCC GVS
Sbjct: 244 NLHNNLLQQVVAKLNNETKQSTFIILDLYNAFLTVFKNKGSNPGSTRFESPLKPCCVGVS 303

Query: 307 SEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDL 347
            E++CGSVDE+G +KY +C +PK+A FWD +HPT EGW + +S LR  L
Sbjct: 304 REYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLRESL 352

BLAST of Sgr026468 vs. ExPASy Swiss-Prot
Match: Q9SF94 (GDSL esterase/lipase At3g09930 OS=Arabidopsis thaliana OX=3702 GN=At3g09930 PE=2 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 2.7e-98
Identity = 178/350 (50.86%), Postives = 236/350 (67.43%), Query Frame = 0

Query: 1   MDLRKLLFPL-IFSLFVFSLLAGQGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASSS 60
           M+L KLL  L +FS   F L A     +H      RP RLFVFGDSY DTGNI  + S S
Sbjct: 1   MELPKLLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDS 60

Query: 61  RNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFGG 120
              PYGITFP  PSGRFSDGRV TD+LA Y  +KSP+P++     G +R ++G+N+A+GG
Sbjct: 61  WKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGG 120

Query: 121 TGVFDT-SFPFPNMTTQIDFFQK-LITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSV 180
           TGVF T   P PNMTTQID+FQ+ L   +I++ +D+ SSLALVS +GNDY+ +LA    +
Sbjct: 121 TGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPL 180

Query: 181 EGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLV 240
                F+  VV+QIAVN  RIH LGV K+V+  + P+GCLP +T  +SF++CNAT N+  
Sbjct: 181 TELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAST 240

Query: 241 EFHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVS 300
             HN LL +A+ +LN+ETK S F +LD Y+  L++ +NKG   G +RF  PL PCC GV+
Sbjct: 241 NLHNYLLHKAIARLNNETKPSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVN 300

Query: 301 SEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLK 348
           S + C +VDE+G +KY +C+DPK+A FWD  HP+ EGW + +S L   LK
Sbjct: 301 SSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLK 350

BLAST of Sgr026468 vs. ExPASy Swiss-Prot
Match: Q9LZS9 (GDSL esterase/lipase At5g03590 OS=Arabidopsis thaliana OX=3702 GN=At5g03590 PE=3 SV=2)

HSP 1 Score: 259.6 bits (662), Expect = 5.0e-68
Identity = 149/349 (42.69%), Postives = 211/349 (60.46%), Query Frame = 0

Query: 7   LFPLIFSLFVFSLLAGQGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASSSRNFPYGI 66
           L  L FSL +F  + G   +      +  P +LFVFG+SY DTGN+  TA S +  PYGI
Sbjct: 4   LMKLFFSLSLFFGINGAVGSNQPSQANKNP-KLFVFGNSYADTGNMKPTALSWK-LPYGI 63

Query: 67  TFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRP--IHGINFAFGGTGVFD 126
           TFPG PSGR+SDG   TD+LA   +L + LP+ +W+  G K+     G+NFAFGG+ VFD
Sbjct: 64  TFPGKPSGRYSDGLTATDFLAK--QLGAKLPY-LWRTHGKKKVKLNRGMNFAFGGSEVFD 123

Query: 127 TSFP-FPNMTTQIDFFQKL-ITNSIFTTN-DIHSSLALVSPSGNDYSFYLATNGSVEGFK 186
           +     PN++TQ+ F   L +   ++T + D+ SS AL+S SG DY  ++  N ++  + 
Sbjct: 124 SPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNPNMAAYP 183

Query: 187 PFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVEFHN 246
            F+  +V  I  +L  ++ L  K + VT L P+GCLP++T  SSF+ CN + + LV  HN
Sbjct: 184 AFVEFIVEDIQYSLGIMNGLKFKNIAVTSLHPLGCLPRVTVASSFRSCNESYSDLVRLHN 243

Query: 247 LLLKQAVEKLNSE----TKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVS 306
             LK+AV KLN E    TKG  F I+DL+   ++I++ KG+     RF++PL PCC G  
Sbjct: 244 ESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKKGN----KRFKSPLKPCCEG-- 303

Query: 307 SEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDL 347
               C  +D +G +KYTLC DPKSA FWD ++PT EGW + +S L   L
Sbjct: 304 ---DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSL 338

BLAST of Sgr026468 vs. ExPASy Swiss-Prot
Match: Q8RWJ4 (GDSL esterase/lipase At2g36325 OS=Arabidopsis thaliana OX=3702 GN=At2g36325 PE=2 SV=2)

HSP 1 Score: 256.1 bits (653), Expect = 5.6e-67
Identity = 142/320 (44.38%), Postives = 188/320 (58.75%), Query Frame = 0

Query: 32  FHFRPTRLFVFGDSYVDTGNINSTASSSRNFPYGITFPGVPSGRFSDGRVLTDYLASYFR 91
           F F+PT+LFVFGDSY DTGN       S  FP GITFPG+P+GRFSDGRV TDYLA Y  
Sbjct: 41  FGFKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIG 100

Query: 92  LKSPLPFSVWQNVGTKRPI--HGINFAFGGTGVFDTSFPF-PNMTTQIDFFQKLITNSIF 151
           +++P+ +  W   G  R     G+NFA+GG G F+T F   P  + QID F++L+  +++
Sbjct: 101 VRTPITYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVY 160

Query: 152 TTNDIHSSLALVSPSGNDYSFYLATNGSVEGFKPFIISVVNQIAVNLKRIHSLGVKKVVV 211
           +  D++SS+A  S  GNDY  Y   NGS EG       VV QI +++KRI  LGV+KV+V
Sbjct: 161 SPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLV 220

Query: 212 TGLGPVGCLPQLTAPSSFKQCNATMNSLVEFHNLLLKQAVEKLNSE---TKGSPFFILDL 271
               P  CLP+L  P   K C+ T ++    HN LL++ + KLN +        F  LDL
Sbjct: 221 ALSPPQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDL 280

Query: 272 YDTLLSIIQNKGSVRGRARFETPLTPCCFGVSSEFSCGSVDEQGNRKYTLCKDPKSALFW 331
           Y+  ++I +NKG V G + F  P   CC      F CG     G + YTLC DPKS  FW
Sbjct: 281 YNAFVTIFKNKG-VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFW 340

Query: 332 DSVHPTHEGWFAAFSSLRSD 346
           D+VH + +GW + FS L  D
Sbjct: 341 DNVHISDQGWRSVFSLLLPD 353

BLAST of Sgr026468 vs. ExPASy Swiss-Prot
Match: Q9LZS8 (GDSL esterase/lipase At5g03600 OS=Arabidopsis thaliana OX=3702 GN=At5g03600 PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.8e-65
Identity = 134/316 (42.41%), Postives = 189/316 (59.81%), Query Frame = 0

Query: 38  RLFVFGDSYVDTGNINSTASSSRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLP 97
           +LFVFGDSY DTGN     + +   PYGITFPG PSGR+ DG + TD+L      +SP  
Sbjct: 14  KLFVFGDSYADTGN-TKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 98  FSV-WQNVGTKRPIHGINFAFGGTGVFDTS--FPFPNMTTQIDFFQKLITNSIFTTNDIH 157
           +    ++ G KR   G+NFAFGG+ + D+S   PFPN+T Q++F   L+       +   
Sbjct: 74  YRTHGRDKGLKR---GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITP 133

Query: 158 SSLALVSPSGNDYSFYLATNGSVEGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPV 217
           S ++L+S +G DY +Y+  N    G K  +  VV+ + VN+  +  L  KK+ VT L P+
Sbjct: 134 SDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQPI 193

Query: 218 GCLPQLTAPSSFKQCNATMNSLVEFHNLLLKQAVEKLNSET----KGSPFFILDLYDTLL 277
           GCLP  T+ SSFK CN + ++LVE HN LLK+ V KLN ++    K   FFI+D+++  +
Sbjct: 194 GCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNAFM 253

Query: 278 SIIQNKGSVRGRARFETPLTPCCFGVSSEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHP 337
           ++++NKGS     RF+ P+  CC G      CG     G + YTLC DPKS  FWD+VHP
Sbjct: 254 TVMKNKGS----KRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDPKSFFFWDAVHP 313

Query: 338 THEGWFAAFSSLRSDL 347
           T EGW + +S L + L
Sbjct: 314 TQEGWRSIYSVLGNPL 315

BLAST of Sgr026468 vs. ExPASy TrEMBL
Match: A0A6J1BU10 (GDSL esterase/lipase At5g03610-like OS=Momordica charantia OX=3673 GN=LOC111005765 PE=3 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 8.4e-167
Identity = 289/351 (82.34%), Postives = 313/351 (89.17%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSAR-HHRHFHFRPTRLFVFGDSYVDTGNINSTASSS 60
           MD +KLLF LIFSL +FSLL GQGSAR HHRHFH RPTRLFVFGDSYVDTGNIN   SS+
Sbjct: 1   MDSKKLLFSLIFSLSLFSLLLGQGSARYHHRHFHLRPTRLFVFGDSYVDTGNINVNTSSA 60

Query: 61  RNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFGG 120
           RN PYGITFPGVPSGR SDGRVLTDYLA YF LKSP+PF+ WQNVG KRP +GINFAFGG
Sbjct: 61  RNIPYGITFPGVPSGRLSDGRVLTDYLARYFGLKSPVPFTTWQNVGVKRPRNGINFAFGG 120

Query: 121 TGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVEG 180
           TG+FDTSFPFPNMTTQI+FFQKLI NSIFT NDIHSS+ALVS SGNDYSFY ATNGS+EG
Sbjct: 121 TGIFDTSFPFPNMTTQINFFQKLIANSIFTLNDIHSSIALVSVSGNDYSFYQATNGSMEG 180

Query: 181 FKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVEF 240
           FKPFI+SVVNQIA+NLKRIH LGV+KVV+ GLGP+GCLP+LTA SSF +CNATM+SLVEF
Sbjct: 181 FKPFIVSVVNQIAINLKRIHGLGVRKVVMMGLGPLGCLPELTAASSFSKCNATMDSLVEF 240

Query: 241 HNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGS-VRGRARFETPLTPCCFGVSS 300
           HN LLKQAVEKLN ETKGSPFFILD+Y TLLSIIQNKGS  + R RFETPL PCCFGVSS
Sbjct: 241 HNALLKQAVEKLNKETKGSPFFILDIYGTLLSIIQNKGSPYKARPRFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKHL 350
           EFSCGSVD++GN+KYTLCKDPK ALFWDSVHPT EGWF AF SL S+LK L
Sbjct: 301 EFSCGSVDKKGNKKYTLCKDPKLALFWDSVHPTQEGWFVAFRSLGSNLKKL 351

BLAST of Sgr026468 vs. ExPASy TrEMBL
Match: A0A6J1HI12 (GDSL esterase/lipase At5g03610-like OS=Cucurbita moschata OX=3662 GN=LOC111463159 PE=3 SV=1)

HSP 1 Score: 540.0 bits (1390), Expect = 7.1e-150
Identity = 264/350 (75.43%), Postives = 293/350 (83.71%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHF--RPTRLFVFGDSYVDTGNINSTASS 60
           M+ RKL+FPL+ SLFVFSLL GQGS R H  + F  RPT+LFVFGDSYVDTGNI +T SS
Sbjct: 1   MESRKLVFPLL-SLFVFSLLLGQGSGRRHVDYRFNSRPTKLFVFGDSYVDTGNIKATTSS 60

Query: 61  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 120
           SRNFPYGITFPGVPSGRFSDGRVLTD+ A Y  LKSP  FS W+  G K P +G+NFAFG
Sbjct: 61  SRNFPYGITFPGVPSGRFSDGRVLTDFFADYLELKSPETFSGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVE 180
           GTGVFDTSFPFPNMTTQIDFFQ L+ N+IF    + SS+ALVSPSGNDYSFY   NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFAPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVE 240
           G +PFIISVVNQIAVNLKRIHSLGVKK+VV GLG VGCLPQ TA SSFK CNAT NS+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIHSLGVKKIVVLGLGAVGCLPQPTANSSFKHCNATSNSMAK 240

Query: 241 FHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVSS 300
            HNLLLKQAVEKLN ETKGSPF I D+Y+ +LSII+NKG+ + RARFETPL PCCFGVSS
Sbjct: 241 VHNLLLKQAVEKLNKETKGSPFIIFDVYNAILSIIRNKGNPKARARFETPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKH 349
           EFSCGSVDE+G + YTLCK PKSALFWDSVHPT +GWFA FSSL S LK+
Sbjct: 301 EFSCGSVDEKGKKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLKY 349

BLAST of Sgr026468 vs. ExPASy TrEMBL
Match: A0A6J1I6N6 (GDSL esterase/lipase At5g03610-like OS=Cucurbita maxima OX=3661 GN=LOC111471216 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.5e-147
Identity = 259/350 (74.00%), Postives = 291/350 (83.14%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHFR--PTRLFVFGDSYVDTGNINSTASS 60
           M+ RK +FPL+ SLFVFSLL G+GS RHH  + F   PT+LFVFGDSYVDTGNI +T SS
Sbjct: 1   MESRKTVFPLL-SLFVFSLLLGRGSGRHHVDYRFNSWPTKLFVFGDSYVDTGNIKATNSS 60

Query: 61  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 120
           SRN PYGITFPGVPSGRFSDGRV+TD+ A+Y  LKSP  F  W+  G K P +G+NFAFG
Sbjct: 61  SRNLPYGITFPGVPSGRFSDGRVMTDFFANYLELKSPETFIGWRKGGRKGPKYGMNFAFG 120

Query: 121 GTGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVE 180
           GTGVFDTSFPFPNMTTQIDFFQ L+ N+IF    + SS+ALVSPSGNDYSFY   NGS+E
Sbjct: 121 GTGVFDTSFPFPNMTTQIDFFQGLVANNIFDPKHVGSSVALVSPSGNDYSFYQERNGSME 180

Query: 181 GFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVE 240
           G +PFIISVVNQIAVNLKRI SLGVKK+VV GLGPVGCLPQ TA SSFK CN+T NS+ +
Sbjct: 181 GLQPFIISVVNQIAVNLKRIQSLGVKKIVVLGLGPVGCLPQPTANSSFKHCNSTTNSMAK 240

Query: 241 FHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVSS 300
            HNLLLKQAVEKLN +TKGSPF I D+Y+ +LSII+NKGS + RARF TPL PCCFGVSS
Sbjct: 241 VHNLLLKQAVEKLNKDTKGSPFIIFDVYNAILSIIRNKGSPKARARFVTPLKPCCFGVSS 300

Query: 301 EFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKH 349
           EFSCGSVDE+GN+ YTLCK PKSALFWDSVHPT +GWFA FSSL S LKH
Sbjct: 301 EFSCGSVDEKGNKMYTLCKRPKSALFWDSVHPTQQGWFATFSSLASSLKH 349

BLAST of Sgr026468 vs. ExPASy TrEMBL
Match: A0A0A0KIM6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G452040 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 8.2e-138
Identity = 256/354 (72.32%), Postives = 288/354 (81.36%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASSSR 60
           M    LLF  + SL +FSL  GQGS+    H HF P +LFVFGDSYVDTGNIN   SS+R
Sbjct: 1   MASNNLLFSFL-SLLLFSLHLGQGSS-SPVHAHFYPLKLFVFGDSYVDTGNINVNTSSAR 60

Query: 61  NFPYGITFPGVPSGRFSDGRVLTDYLASYFRLK-SPLPFSVWQNVGTK-RPIHGINFAFG 120
           NFPYGITFPG PSGRFSDGRVLTD+LA+Y  LK SP+PF+ W+  G+K R   GINFAFG
Sbjct: 61  NFPYGITFPGFPSGRFSDGRVLTDFLANYVGLKRSPIPFTAWKKTGSKLRAKCGINFAFG 120

Query: 121 GTGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVE 180
           GTGVFDT +PFPNMTTQI+FFQ LI NSIFT+ DIHSS+ALVSPSGNDYSFYLATNGS E
Sbjct: 121 GTGVFDTLYPFPNMTTQINFFQNLIANSIFTSYDIHSSIALVSPSGNDYSFYLATNGSPE 180

Query: 181 GFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVE 240
           G KPF+ISVVNQI+VNLKRI+ LGVKK+VV GLGPVGC P  TAP SFK+CN TMNSL  
Sbjct: 181 GVKPFVISVVNQISVNLKRIYKLGVKKIVVVGLGPVGCYPSPTAP-SFKKCNETMNSLAV 240

Query: 241 FHNLLLKQAVEKLNSETK--GSP-FFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFG 300
           FHN LLKQAVEKLNSETK  GSP FFIL++YDT+LSII+NKG+ +  A F+TPL PCCFG
Sbjct: 241 FHNTLLKQAVEKLNSETKLGGSPNFFILNMYDTVLSIIKNKGNPKVGATFQTPLKPCCFG 300

Query: 301 VSSEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKHL 350
           VSS FSCGSVDE GN+ YTLCK P  ALFWD+VHPT +GWFA F+SL S LK +
Sbjct: 301 VSSNFSCGSVDEHGNKMYTLCKRPDLALFWDTVHPTQKGWFATFNSLISTLKRI 351

BLAST of Sgr026468 vs. ExPASy TrEMBL
Match: A0A1S3CH67 (GDSL esterase/lipase At5g03610-like OS=Cucumis melo OX=3656 GN=LOC103500887 PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 5.3e-137
Identity = 250/352 (71.02%), Postives = 285/352 (80.97%), Query Frame = 0

Query: 1   MDLRKLLFPLIFSLFVFSLLAGQGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASSSR 60
           M   KLLF  +    VF L  GQGS+      HF P +LFVFGDSYVDTGNI+  ASS+R
Sbjct: 1   MASNKLLFSFL-PFLVFFLHFGQGSSA----VHFHPLKLFVFGDSYVDTGNIHVNASSAR 60

Query: 61  NFPYGITFPGVPSGRFSDGRVLTDYLASYFRL-KSPLPFSVWQNVGTKRPIHGINFAFGG 120
           NFPYGITFPGVPSGRFSDGRVLTD+LA Y  L KSP+PF+VWQ  G K   +G+NF FGG
Sbjct: 61  NFPYGITFPGVPSGRFSDGRVLTDFLAKYVGLKKSPIPFTVWQRFGRKGAKYGMNFGFGG 120

Query: 121 TGVFDTSFPFPNMTTQIDFFQKLITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSVEG 180
           TGVFDTSFPFPNMTTQI+FF+ LI NSIF++ DIHSS+ALVSPSGNDYSFY   NGS+EG
Sbjct: 121 TGVFDTSFPFPNMTTQINFFRNLIVNSIFSSYDIHSSIALVSPSGNDYSFYFTNNGSLEG 180

Query: 181 FKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLVEF 240
           FKPFI SVVNQI+VNLKRI+ LGVKKVVV GLGPVGC P+ TAP SFK+CN TM+S+  F
Sbjct: 181 FKPFIFSVVNQISVNLKRIYKLGVKKVVVLGLGPVGCYPKPTAP-SFKKCNKTMDSIAVF 240

Query: 241 HNLLLKQAVEKLNSETK-GSP-FFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVS 300
           HN LLKQAVEKLN ETK GSP FFIL++YDT+LSII+NK + +G A F+TPL PCCFGV+
Sbjct: 241 HNTLLKQAVEKLNYETKEGSPNFFILNMYDTVLSIIKNKENRKGGATFKTPLKPCCFGVN 300

Query: 301 SEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLKHL 350
           S FSCGSVDE+GN+ YTLCK P  ALFWD+VHPT  GWFA FSS +S LKH+
Sbjct: 301 SNFSCGSVDERGNKMYTLCKRPDLALFWDAVHPTQNGWFATFSSFKSTLKHI 346

BLAST of Sgr026468 vs. TAIR 10
Match: AT5G03610.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 394.0 bits (1011), Expect = 1.2e-109
Identity = 195/349 (55.87%), Postives = 246/349 (70.49%), Query Frame = 0

Query: 7   LFPLIFSLFVF---SLLAG-----QGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASS 66
           L  L F LF+F   SLL G     +GS ++H  + FRPT+LFVFGDSY DTGNI    SS
Sbjct: 4   LIKLFFCLFIFLCTSLLFGEINGVEGSNQNHHLYPFRPTKLFVFGDSYADTGNIKKAFSS 63

Query: 67  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 126
           S  FPYGITFPG P+GRFSDGRV TD+LA +  +KSP+P+      G KR  +G+NFA+G
Sbjct: 64  SWKFPYGITFPGKPAGRFSDGRVATDFLAKFVGIKSPIPYFWKDYAGKKRLQYGMNFAYG 123

Query: 127 GTGVFDTSFPFPNMTTQIDFFQKLI-TNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSV 186
           GTGVF+T  P PNMTTQID FQ ++ T  I+   ++ SS+ALVS +GNDYS ++A N   
Sbjct: 124 GTGVFNTQTPLPNMTTQIDIFQNILTTGDIYYPPELTSSVALVSVAGNDYSNFIALNRPA 183

Query: 187 EGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLV 246
             F  FI  VV+Q  VNL+RIH+LGVKK+ V  L P+GCLP  T  +SF++CN T N+LV
Sbjct: 184 SEFPAFIKQVVDQTEVNLRRIHALGVKKIAVPSLQPLGCLPPFTFVTSFQRCNETQNALV 243

Query: 247 EFHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVS 306
             HN LL+Q V KLN+ETK S F ILDLY+  L++ +NKGS  G  RFE+PL PCC GVS
Sbjct: 244 NLHNNLLQQVVAKLNNETKQSTFIILDLYNAFLTVFKNKGSNPGSTRFESPLKPCCVGVS 303

Query: 307 SEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDL 347
            E++CGSVDE+G +KY +C +PK+A FWD +HPT EGW + +S LR  L
Sbjct: 304 REYNCGSVDEKGVKKYIVCDNPKTAFFWDGLHPTEEGWRSVYSVLRESL 352

BLAST of Sgr026468 vs. TAIR 10
Match: AT3G09930.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 1.9e-99
Identity = 178/350 (50.86%), Postives = 236/350 (67.43%), Query Frame = 0

Query: 1   MDLRKLLFPL-IFSLFVFSLLAGQGSARHHRHFHFRPTRLFVFGDSYVDTGNINSTASSS 60
           M+L KLL  L +FS   F L A     +H      RP RLFVFGDSY DTGNI  + S S
Sbjct: 1   MELPKLLISLFLFSFSSFFLGAESDYPQHSNLKRLRPNRLFVFGDSYADTGNIRKSLSDS 60

Query: 61  RNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFGG 120
              PYGITFP  PSGRFSDGRV TD+LA Y  +KSP+P++     G +R ++G+N+A+GG
Sbjct: 61  WKIPYGITFPQKPSGRFSDGRVATDFLARYLGIKSPIPYTWKDYAGKERLLYGMNYAYGG 120

Query: 121 TGVFDT-SFPFPNMTTQIDFFQK-LITNSIFTTNDIHSSLALVSPSGNDYSFYLATNGSV 180
           TGVF T   P PNMTTQID+FQ+ L   +I++ +D+ SSLALVS +GNDY+ +LA    +
Sbjct: 121 TGVFKTKDNPLPNMTTQIDYFQRVLAAGNIYSPSDLPSSLALVSVAGNDYATFLALKRPL 180

Query: 181 EGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNSLV 240
                F+  VV+QIAVN  RIH LGV K+V+  + P+GCLP +T  +SF++CNAT N+  
Sbjct: 181 TELPAFMKQVVDQIAVNAMRIHKLGVNKIVIPSMQPLGCLPSITVFNSFQRCNATDNAST 240

Query: 241 EFHNLLLKQAVEKLNSETKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTPCCFGVS 300
             HN LL +A+ +LN+ETK S F +LD Y+  L++ +NKG   G +RF  PL PCC GV+
Sbjct: 241 NLHNYLLHKAIARLNNETKPSTFVVLDHYNAFLTVFKNKGPEPGVSRFGNPLKPCCVGVN 300

Query: 301 SEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDLK 348
           S + C +VDE+G +KY +C+DPK+A FWD  HP+ EGW + +S L   LK
Sbjct: 301 SSYDCSNVDEKGEKKYIICEDPKAAFFWDIFHPSEEGWRSVYSVLHKHLK 350

BLAST of Sgr026468 vs. TAIR 10
Match: AT2G36325.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 256.1 bits (653), Expect = 4.0e-68
Identity = 142/320 (44.38%), Postives = 188/320 (58.75%), Query Frame = 0

Query: 32  FHFRPTRLFVFGDSYVDTGNINSTASSSRNFPYGITFPGVPSGRFSDGRVLTDYLASYFR 91
           F F+PT+LFVFGDSY DTGN       S  FP GITFPG+P+GRFSDGRV TDYLA Y  
Sbjct: 41  FGFKPTKLFVFGDSYADTGNTPFLIVPSWRFPNGITFPGIPTGRFSDGRVSTDYLAKYIG 100

Query: 92  LKSPLPFSVWQNVGTKRPI--HGINFAFGGTGVFDTSFPF-PNMTTQIDFFQKLITNSIF 151
           +++P+ +  W   G  R     G+NFA+GG G F+T F   P  + QID F++L+  +++
Sbjct: 101 VRTPITYK-WGKYGRPRLAVKRGMNFAYGGAGAFETMFKLVPTASVQIDSFEQLLMRNVY 160

Query: 152 TTNDIHSSLALVSPSGNDYSFYLATNGSVEGFKPFIISVVNQIAVNLKRIHSLGVKKVVV 211
           +  D++SS+A  S  GNDY  Y   NGS EG       VV QI +++KRI  LGV+KV+V
Sbjct: 161 SPADLNSSVAFFSIIGNDYLTYDRRNGSEEGRSALTRKVVKQILLDVKRIKDLGVRKVLV 220

Query: 212 TGLGPVGCLPQLTAPSSFKQCNATMNSLVEFHNLLLKQAVEKLNSE---TKGSPFFILDL 271
               P  CLP+L  P   K C+ T ++    HN LL++ + KLN +        F  LDL
Sbjct: 221 ALSPPQKCLPKLVTP---KGCD-TNDTSTYLHNSLLRKGLIKLNDKEINNNDKSFMTLDL 280

Query: 272 YDTLLSIIQNKGSVRGRARFETPLTPCCFGVSSEFSCGSVDEQGNRKYTLCKDPKSALFW 331
           Y+  ++I +NKG V G + F  P   CC      F CG     G + YTLC DPKS  FW
Sbjct: 281 YNAFVTIFKNKG-VSGVSTFPDPFKACCATKRGTF-CGDRSLSGKKLYTLCDDPKSFFFW 340

Query: 332 DSVHPTHEGWFAAFSSLRSD 346
           D+VH + +GW + FS L  D
Sbjct: 341 DNVHISDQGWRSVFSLLLPD 353

BLAST of Sgr026468 vs. TAIR 10
Match: AT5G03600.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 4.8e-66
Identity = 134/316 (42.41%), Postives = 189/316 (59.81%), Query Frame = 0

Query: 38  RLFVFGDSYVDTGNINSTASSSRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLP 97
           +LFVFGDSY DTGN     + +   PYGITFPG PSGR+ DG + TD+L      +SP  
Sbjct: 14  KLFVFGDSYADTGN-TKRDTEAWAIPYGITFPGKPSGRYCDGLIATDFLEKVLGAESPYL 73

Query: 98  FSV-WQNVGTKRPIHGINFAFGGTGVFDTS--FPFPNMTTQIDFFQKLITNSIFTTNDIH 157
           +    ++ G KR   G+NFAFGG+ + D+S   PFPN+T Q++F   L+       +   
Sbjct: 74  YRTHGRDKGLKR---GMNFAFGGSKMLDSSPNSPFPNITAQVNFLVDLVLAGRVYGDITP 133

Query: 158 SSLALVSPSGNDYSFYLATNGSVEGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPV 217
           S ++L+S +G DY +Y+  N    G K  +  VV+ + VN+  +  L  KK+ VT L P+
Sbjct: 134 SDVSLISYAGGDYIYYIDQNRPAAGLKALVEKVVDNLRVNMIVLGGLLFKKIAVTSLQPI 193

Query: 218 GCLPQLTAPSSFKQCNATMNSLVEFHNLLLKQAVEKLNSET----KGSPFFILDLYDTLL 277
           GCLP  T+ SSFK CN + ++LVE HN LLK+ V KLN ++    K   FFI+D+++  +
Sbjct: 194 GCLPSYTSASSFKSCNESQSALVELHNKLLKKVVAKLNEQSRVMKKEQHFFIIDIHNAFM 253

Query: 278 SIIQNKGSVRGRARFETPLTPCCFGVSSEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHP 337
           ++++NKGS     RF+ P+  CC G      CG     G + YTLC DPKS  FWD+VHP
Sbjct: 254 TVMKNKGS----KRFKNPMKSCCEGY-----CGR-SSDGGKLYTLCDDPKSFFFWDAVHP 313

Query: 338 THEGWFAAFSSLRSDL 347
           T EGW + +S L + L
Sbjct: 314 TQEGWRSIYSVLGNPL 315

BLAST of Sgr026468 vs. TAIR 10
Match: AT5G03590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 154.5 bits (389), Expect = 1.6e-37
Identity = 100/295 (33.90%), Postives = 139/295 (47.12%), Query Frame = 0

Query: 59  SRNFPYGITFPGVPSGRFSDGRVLTDYLASYFRLKSPLPFSVWQNVGTKRPIHGINFAFG 118
           S   PYGITFPG PSGR+SDG   TD+L                                
Sbjct: 7   SWKLPYGITFPGKPSGRYSDGLTATDFL-------------------------------- 66

Query: 119 GTGVFDTSFP-FPNMTTQIDFFQKL-ITNSIFTTN-DIHSSLALVSPSGNDYSFYLATNG 178
           G+ VFD+     PN++TQ+ F   L +   ++T + D+ SS AL+S SG DY  ++  N 
Sbjct: 67  GSEVFDSPVDRSPNISTQVGFLVNLALARRVYTIDGDLASSYALLSYSGTDYYGFIDQNP 126

Query: 179 SVEGFKPFIISVVNQIAVNLKRIHSLGVKKVVVTGLGPVGCLPQLTAPSSFKQCNATMNS 238
           ++  +  F+  +V  I                                     CN + + 
Sbjct: 127 NMAAYPAFVEFIVEDI------------------------------------HCNESYSD 186

Query: 239 LVEFHNLLLKQAVEKLNSE----TKGSPFFILDLYDTLLSIIQNKGSVRGRARFETPLTP 298
           LV  HN  LK+AV KLN E    TKG  F I+DL+   ++I++ KG+     RF++PL P
Sbjct: 187 LVRLHNESLKKAVAKLNKEDKFRTKGDRFVIVDLHKAFMTILEKKGN----KRFKSPLKP 224

Query: 299 CCFGVSSEFSCGSVDEQGNRKYTLCKDPKSALFWDSVHPTHEGWFAAFSSLRSDL 347
           CC G      C  +D +G +KYTLC DPKSA FWD ++PT EGW + +S L   L
Sbjct: 247 CCEG-----DCARMDMKGAKKYTLCNDPKSAFFWDEINPTQEGWRSIYSLLGKSL 224