Homology
BLAST of Sgr026112 vs. NCBI nr
Match:
XP_022132566.1 (L-ascorbate oxidase [Momordica charantia])
HSP 1 Score: 1041.2 bits (2691), Expect = 5.8e-300
Identity = 500/604 (82.78%), Postives = 525/604 (86.92%), Query Frame = 0
Query: 1 MASLLQS--RRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTIN 60
MAS+LQS R ILL LLCV V +FL ESP AEART YYKWEV+ EFKSPDCYKKLAMTIN
Sbjct: 34 MASVLQSRGRGILLILLCVLV-IFLLESPAAEARTLYYKWEVRSEFKSPDCYKKLAMTIN 93
Query: 61 GRTPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGET 120
GRTPGPTIYA QGDT++V+LKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGET
Sbjct: 94 GRTPGPTIYAHQGDTIIVDLKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGET 153
Query: 121 FKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKS 180
FKYQFVVDRPGTYLYHAHYGMQRE+GLYGSI+V LPEGQSEPFSYDYDR+MI TDWYH S
Sbjct: 154 FKYQFVVDRPGTYLYHAHYGMQREEGLYGSIIVWLPEGQSEPFSYDYDRNMIFTDWYHNS 213
Query: 181 SQEHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRK 240
S EHAAGLA PGNGFTWVGEPD +L+ G +
Sbjct: 214 SHEHAAGLAAPGNGFTWVGEPDS------------------LLIQG-------------R 273
Query: 241 GEIQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIM 300
G +G SG CNSSSPEC PHAL VVPGKTYRLRVSSLTSLSALSF+IEGH M
Sbjct: 274 GRFNCSQLGSSPSESGRCNSSSPECFPHALAVVPGKTYRLRVSSLTSLSALSFEIEGHNM 333
Query: 301 TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIF 360
TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPAT PGLAIF
Sbjct: 334 TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATAPGLAIF 393
Query: 361 NYYPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNT 420
NYYPNHQRR PPT P AGPAWDDVKPRLAQSHAIKAR+GFIHAPPKT D+VIVFLNTQNT
Sbjct: 394 NYYPNHQRRPPPTTPXAGPAWDDVKPRLAQSHAIKARRGFIHAPPKTTDRVIVFLNTQNT 453
Query: 421 VNGHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATV 480
V G RRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFK YDIF PAPNPEATV
Sbjct: 454 VKGRRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKGYDIFNPAPNPEATV 513
Query: 481 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYN 540
SDAIYRLDFN+TVD+ILQNAN M+ NNSETHPWHLHGHDFWVLGYGEGKFD+Y+DPS YN
Sbjct: 514 SDAIYRLDFNATVDVILQNANTMNSNNSETHPWHLHGHDFWVLGYGEGKFDVYKDPSGYN 573
Query: 541 LENPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPS 600
LENPIMKNTV +HP+GWTALR VADNPGVWAFHCHI+AHFFMGMGVVFEEGI KVG+LPS
Sbjct: 574 LENPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFFMGMGVVFEEGIHKVGRLPS 605
Query: 601 SIMG 603
SIMG
Sbjct: 634 SIMG 605
BLAST of Sgr026112 vs. NCBI nr
Match:
XP_038881120.1 (L-ascorbate oxidase [Benincasa hispida])
HSP 1 Score: 1007.3 bits (2603), Expect = 9.2e-290
Identity = 487/602 (80.90%), Postives = 515/602 (85.55%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MASLLQSR+ LL LLC +FL +SP AE+RT YKWEVK E KSPDC+KKL MTINGR
Sbjct: 18 MASLLQSRQYLLILLC--FLLFLIQSPIAESRTLNYKWEVKSELKSPDCFKKLTMTINGR 77
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNS TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK
Sbjct: 78 SPGPTIYAHQGDTVIVELKNSFSTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 137
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV LPEGQ+EPFSYDYDR++ILTDWYHK+S
Sbjct: 138 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFSYDYDRNLILTDWYHKTSH 197
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHAAGLATPG+GFTWVGEPD +L+ G F SV
Sbjct: 198 EHAAGLATPGSGFTWVGEPDS------------------LLIQGRGRFNCSV-------- 257
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
S TS CNSS+PEC+ + +TVVPGKTYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 258 -------LGSSTSATCNSSNPECILYPITVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 317
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYW T+VVSR TPPGLAIFNY
Sbjct: 318 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 377
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQR+ PPT PPA PAW+DV PRLAQSHAIKARQGFIHAPPK ADKVIV LNTQNTVN
Sbjct: 378 YPNHQRKLPPTTPPAPPAWNDVAPRLAQSHAIKARQGFIHAPPKAADKVIVLLNTQNTVN 437
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLH FSQ KPPTGYDFKNYDIF PAPNPEATVSD
Sbjct: 438 GRRRWSLNNVSFNMPHTPYLIALKHNLLHAFSQDKPPTGYDFKNYDIFNPAPNPEATVSD 497
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AI+RLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY+DPSK+NLE
Sbjct: 498 AIFRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYKDPSKFNLE 557
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVF EGI +V KLP+SI
Sbjct: 558 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFAEGIDRVQKLPTSI 584
Query: 601 MG 603
MG
Sbjct: 618 MG 584
BLAST of Sgr026112 vs. NCBI nr
Match:
XP_008440636.1 (PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase [Cucumis melo var. makuwa] >TYK12674.1 L-ascorbate oxidase [Cucumis melo var. makuwa])
HSP 1 Score: 999.2 bits (2582), Expect = 2.5e-287
Identity = 485/602 (80.56%), Postives = 514/602 (85.38%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MASLLQ ++ L LL F+FL +S TAE+RT YKWEVK E KSPDC+KKL +TINGR
Sbjct: 1 MASLLQRKQYFLLLLS--SFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGR 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+
Sbjct: 61 SPGPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFR 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV +PEGQ+EPFSYD+DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSN 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHAAGLATPG+GFTWVGEPD +L+ G F SV S
Sbjct: 181 EHAAGLATPGSGFTWVGEPDS------------------LLIQGRGRFNCSVLGSS---- 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
SG CNSSS ECV + LTVVPGKTYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 241 ------------SGTCNSSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHSMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYW T+VVSR TPPGLAIFNY
Sbjct: 301 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQ + PPT PPA PAW+DV PRLAQS AIKARQGFIHAPPK ADKVIV LNTQNTVN
Sbjct: 361 YPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVN 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQ KPPTGYDFKNYDIF PAPNPEATVSD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY DPSKYNLE
Sbjct: 481 AIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLE 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI++VGKLP+SI
Sbjct: 541 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 566
Query: 601 MG 603
MG
Sbjct: 601 MG 566
BLAST of Sgr026112 vs. NCBI nr
Match:
XP_004143482.1 (L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_003073 [Cucumis sativus])
HSP 1 Score: 998.0 bits (2579), Expect = 5.6e-287
Identity = 487/602 (80.90%), Postives = 512/602 (85.05%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MAS LQS R LL LL FVF FL +S TAE+RT YKWEVK E KSPDC+KK+ +TINGR
Sbjct: 1 MASPLQSTRYLLILLS-FVF-FLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGR 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+
Sbjct: 61 SPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFR 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV +PEGQ+EPFSYD DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSH 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHA GLATPG+GF WVGEPD +L+ G F S+ S
Sbjct: 181 EHATGLATPGSGFNWVGEPDS------------------LLIQGRGRFNCSILGSS---- 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
SG CNSSS ECV H LTVVPGKTYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 241 ------------SGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYW T+VVSR TPPGLAIFNY
Sbjct: 301 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQ R PPT PPA PAW+DV PRLAQS AIKARQGFIH PPK ADKVIV LNTQNTVN
Sbjct: 361 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQ KPPTGYDFKNYDIF PAPNPEATVSD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFD+Y+DPSKYNLE
Sbjct: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI++VGKLP+SI
Sbjct: 541 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 566
Query: 601 MG 603
MG
Sbjct: 601 MG 566
BLAST of Sgr026112 vs. NCBI nr
Match:
KAG6594863.1 (L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826.1 L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 983.8 bits (2542), Expect = 1.1e-282
Identity = 474/602 (78.74%), Postives = 511/602 (84.88%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
M SLLQSR+ LL LLC FVFL+ES TAE+RT YYKWEVK E KSPDC+KKL +TINGR
Sbjct: 1 MGSLLQSRQYLLILLC--FFVFLWESRTAESRTLYYKWEVKSELKSPDCFKKLTLTINGR 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNS TENVAIHWHGIRQIGTPWSDGTEGVTQCP+LPGETFK
Sbjct: 61 SPGPTIYAHQGDTVIVELKNSFVTENVAIHWHGIRQIGTPWSDGTEGVTQCPVLPGETFK 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV LPEGQ+EPF+YD+DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWLPEGQTEPFAYDHDRNLILTDWYHKSSH 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EH+AGLA G+ FTWVGEPD +L+ G +G
Sbjct: 181 EHSAGLAAAGSAFTWVGEPDS------------------LLIQG-------------RGR 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
+G S CNSSSP+CV + +TVVPG+TYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 241 FNCSLLG----ASATCNSSSPDCVLYPITVVPGRTYRLRVSSLTSLSALSFQIEGHTMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQ+PSRNYW T+VVSR TP GLAIFNY
Sbjct: 301 VEADGHYVEPFQVSNLFIYSGETYSVLIKADQNPSRNYWITTNVVSRNSTTPLGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQR+ PPT PPAGPAW+ V PRLAQSHAIKARQGFIH PPK ADKVIV LNTQNTVN
Sbjct: 361 YPNHQRKFPPTTPPAGPAWNAVAPRLAQSHAIKARQGFIHPPPKAADKVIVLLNTQNTVN 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQAKPPTGYDFKNY+IF P PNPEATVSD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKNNLLHTFSQAKPPTGYDFKNYNIFVPPPNPEATVSD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AIYRLDFNSTVDIILQNANMM+ N SETHPWHLHGHDFWVLGYGEGKFD+++DPSKYNL
Sbjct: 481 AIYRLDFNSTVDIILQNANMMALNTSETHPWHLHGHDFWVLGYGEGKFDMFKDPSKYNLV 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHP+GWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI+KV KLP+SI
Sbjct: 541 NPIMKNTVPLHPFGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIEKVQKLPTSI 565
Query: 601 MG 603
MG
Sbjct: 601 MG 565
BLAST of Sgr026112 vs. ExPASy Swiss-Prot
Match:
P14133 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1)
HSP 1 Score: 580.1 bits (1494), Expect = 4.8e-164
Identity = 288/601 (47.92%), Postives = 373/601 (62.06%), Query Frame = 0
Query: 17 VFVFVFLFESPTAEA---RTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDT 76
V +F F +EA + ++YKW+V+Y F SPDC + + M ING PGPTI A GD
Sbjct: 19 VLSIIFGFGITLSEAGFPKIKHYKWDVEYMFWSPDCVENIVMGINGEFPGPTIRANAGDI 78
Query: 77 VVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLY 136
VVVEL N L TE V IHWHGI Q GTPW+DGT ++QC I PGETF Y+FVVD+ GTY Y
Sbjct: 79 VVVELTNKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFTYRFVVDKAGTYFY 138
Query: 137 HAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGF 196
H H GMQR GLYGS++V PEG+SEPF YD + +++L+DW+H+S + GL++
Sbjct: 139 HGHLGMQRSAGLYGSLIVDPPEGRSEPFHYDEEINLLLSDWWHQSVHKQEVGLSS--KPM 198
Query: 197 TWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSLTS 256
W+GEP +L+ G F S+ +G Q
Sbjct: 199 RWIGEPQS------------------ILINGKGQFDCSIAAKYNQGLKQ----------- 258
Query: 257 GLCNSSSPE-CVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQ 316
C S E C P L V P KTYR+R++S T+L++L+F I H + VVEADG+YV+PF
Sbjct: 259 --CELSGKEKCAPFILHVQPKKTYRIRIASTTALASLNFAIGNHELLVVEADGNYVQPFV 318
Query: 317 VKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTE 376
++ IYSGE+YSVLI DQ+P NYW + V +R P TPPGL + NY PN + P +
Sbjct: 319 TSDIDIYSGESYSVLITTDQNPLENYWVSIGVRARLPKTPPGLTLLNYLPNSASKLPISP 378
Query: 377 PPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSF 436
PP P W+D + I A G PP ++ + LNTQN +NG +W++NNVS
Sbjct: 379 PPETPHWEDFDRSKNFTFRIFAAMG-SPKPPVRYNRRLFLLNTQNRINGFMKWAINNVSL 438
Query: 437 NMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVD 496
+P TPYL ++K L F+Q PP + NYDI P PNPE T + +Y+ + TVD
Sbjct: 439 ALPPTPYLAAMKMRLNTAFNQNPPPETFPL-NYDINNPPPNPETTTGNGVYKFNMGETVD 498
Query: 497 IILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHP 556
+ILQNANM++PN SE HPWHLHGHDFWVLGYGEGKF +D K NL+NP ++NTV + P
Sbjct: 499 VILQNANMLNPNMSEIHPWHLHGHDFWVLGYGEGKFYAPEDEKKLNLKNPPLRNTVVIFP 558
Query: 557 YGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIMGLLSISIGPYF 614
YGWTA+R VADNPGVWAFHCHI+ H MGMGVVF EG+ VG +P + S ++ +
Sbjct: 559 YGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVHMVGMIPPKALACGSTALVKNY 584
BLAST of Sgr026112 vs. ExPASy Swiss-Prot
Match:
P24792 (L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2)
HSP 1 Score: 574.7 bits (1480), Expect = 2.0e-162
Identity = 283/603 (46.93%), Postives = 377/603 (62.52%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
M + ++R +L F + F + ++ R+YKWEV+Y F +PDC + + M ING+
Sbjct: 1 MLQMGKAREPNFLILFFFGLILAFGISSEGSQIRHYKWEVEYMFWAPDCNENIVMGINGQ 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
PGPTI A GDTVVVEL N L TE V IHWHGI Q GTPW+DGT ++QC I PGETF
Sbjct: 61 FPGPTIRANAGDTVVVELINKLHTEGVVIHWHGILQRGTPWADGTASISQCAINPGETFF 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
Y F VD PGT+ YH H GMQR GLYGS++V P+G+ EPF YD + +++L+DW+H+S
Sbjct: 121 YNFTVDNPGTFFYHGHLGMQRSAGLYGSLIVDPPQGKKEPFHYDGEINLLLSDWWHQSIH 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVT--FDSRK 240
+ GL++ W+GEP +LL G F S+ +DS
Sbjct: 181 KQEVGLSS--KPIRWIGEPQT------------------ILLNGRGQFDCSIAAKYDSNL 240
Query: 241 GEIQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIM 300
+L S C P+ V+P KTYR+R++S T+L+AL+F I H +
Sbjct: 241 EPCKL--------------KGSEPCAPYIFHVMPKKTYRIRIASTTALAALNFAIGNHPL 300
Query: 301 TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIF 360
VVEADG+YV+PF ++ IYSGE+YSVLI DQ+PS NYW + R P TPPGL +
Sbjct: 301 LVVEADGNYVQPFYTSDIDIYSGESYSVLITTDQNPSENYWVSVGTRGRHPNTPPGLTLL 360
Query: 361 NYYPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNT 420
NY PN + P + PP PAWDD ++ I A G PP +++ I LNTQN
Sbjct: 361 NYLPNSVSKLPTSPPPETPAWDDFDRSKNFTYRITAAMG-SPKPPVKSNRRIFLLNTQNV 420
Query: 421 VNGHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATV 480
+NG+ +W++N+VS +P TPYL ++K NLLH F Q PP + ++YDI P N + +
Sbjct: 421 INGYVKWAINDVSLALPPTPYLGAMKFNLLHAFDQNPPPEVFP-EDYDIDTPPTNEKTKI 480
Query: 481 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYN 540
+ +Y+ VD+ILQNANMM N SE HPWHLHGHDFWVLGYG+GKF ++ S N
Sbjct: 481 GNGVYQFKIGEIVDVILQNANMMKENLSEIHPWHLHGHDFWVLGYGDGKFTA-EEESSLN 540
Query: 541 LENPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPS 600
L+NP ++NTV + PYGWTA+R VADNPGVWAFHCHI+ H MGMGVVF EG++KVG++P+
Sbjct: 541 LKNPPLRNTVVIFPYGWTAIRFVADNPGVWAFHCHIEPHLHMGMGVVFAEGVEKVGRIPT 566
Query: 601 SIM 602
+
Sbjct: 601 KAL 566
BLAST of Sgr026112 vs. ExPASy Swiss-Prot
Match:
P37064 (L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1)
HSP 1 Score: 573.9 bits (1478), Expect = 3.4e-162
Identity = 277/573 (48.34%), Postives = 366/573 (63.87%), Query Frame = 0
Query: 31 ARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDTVVVELKNSLGTENVAIH 90
++ R+YKWEV+Y F +P+C + + M ING+ PGPTI A GD+VVVEL N L TE V IH
Sbjct: 1 SQIRHYKWEVEYMFWAPNCNENIVMGINGQFPGPTIRANAGDSVVVELTNKLHTEGVVIH 60
Query: 91 WHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 150
WHGI Q GTPW+DGT ++QC I PGETF Y F VD PGT+ YH H GMQR GLYGS++
Sbjct: 61 WHGILQRGTPWADGTASISQCAINPGETFFYNFTVDNPGTFFYHGHLGMQRSAGLYGSLI 120
Query: 151 VSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGFTWVGEPDVRKLLPFPLF 210
V P+G+ EPF YD + +++L+DW+H+S + GL++ W+GEP
Sbjct: 121 VDPPQGKKEPFHYDGEINLLLSDWWHQSIHKQEVGLSS--KPIRWIGEPQT--------- 180
Query: 211 IPAIKHMDWVLLMGFFLFILSVT--FDSRKGEIQLLSVGFKSLTSGLCNSSSPECVPHAL 270
+LL G F S+ +DS +L S C P+
Sbjct: 181 ---------ILLNGRGQFDCSIAAKYDSNLEPCKL--------------KGSESCAPYIF 240
Query: 271 TVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQVKNLFIYSGETYSVLI 330
V P KTYR+R++S T+L+AL+F I H + VVEADG+YV+PF ++ IYSGE+YSVLI
Sbjct: 241 HVSPKKTYRIRIASTTALAALNFAIGNHQLLVVEADGNYVQPFYTSDIDIYSGESYSVLI 300
Query: 331 KADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEPPAGPAWDDVKPRLAQ 390
DQ+PS NYW + +R P TPPGL + NY PN + P + PP PAWDD
Sbjct: 301 TTDQNPSENYWVSVGTRARHPNTPPGLTLLNYLPNSVSKLPTSPPPQTPAWDDFDRSKNF 360
Query: 391 SHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFNMPHTPYLISLKKNLL 450
++ I A G PP ++ I LNTQN +NG+ +W++N+VS +P TPYL ++K NLL
Sbjct: 361 TYRITAAMG-SPKPPVKFNRRIFLLNTQNVINGYVKWAINDVSLALPPTPYLGAMKYNLL 420
Query: 451 HTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNSET 510
H F Q PP + ++YDI P N + + + +Y+ VD+ILQNANMM N SET
Sbjct: 421 HAFDQNPPPEVFP-EDYDIDTPPTNEKTRIGNGVYQFKIGEVVDVILQNANMMKENLSET 480
Query: 511 HPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPYGWTALRIVADNPGVW 570
HPWHLHGHDFWVLGYG+GKF ++ S NL+NP ++NTV + PYGWTA+R VADNPGVW
Sbjct: 481 HPWHLHGHDFWVLGYGDGKFSA-EEESSLNLKNPPLRNTVVIFPYGWTAIRFVADNPGVW 536
Query: 571 AFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIM 602
AFHCHI+ H MGMGVVF EG++KVG++P+ +
Sbjct: 541 AFHCHIEPHLHMGMGVVFAEGVEKVGRIPTKAL 536
BLAST of Sgr026112 vs. ExPASy Swiss-Prot
Match:
M4DUF2 (L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1)
HSP 1 Score: 561.6 bits (1446), Expect = 1.8e-158
Identity = 273/576 (47.40%), Postives = 358/576 (62.15%), Query Frame = 0
Query: 28 TAEARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDTVVVELKNSLGTENV 87
TA A R Y WEV+Y+F PDC + + M +NG+ PGPTI+A GDT+VV L N L TE +
Sbjct: 15 TASAAVREYAWEVEYKFGWPDCKEGMVMAVNGQFPGPTIHALAGDTIVVHLTNKLATEGL 74
Query: 88 AIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYG 147
IHWHGIRQ+G+PW+DG GVTQC I PGETF Y F VD+PGT+ YH HYGMQR GLYG
Sbjct: 75 VIHWHGIRQLGSPWADGAAGVTQCAISPGETFTYNFTVDKPGTHFYHGHYGMQRSAGLYG 134
Query: 148 SIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGFTWVGEPDVRKLLPF 207
S+++ + +G+ EP YD + +++L+DW+H+ GL++ W+GE
Sbjct: 135 SLIIDVAKGKKEPLRYDGEFNLLLSDWWHEDVLSQEIGLSS--RPMRWIGEAQS------ 194
Query: 208 PLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSLTSGLCN-SSSPECVPH 267
+L+ G F S L+ F S + C +C P
Sbjct: 195 ------------ILINGRGQFNCS------------LAAQFSSTSLPTCTFKEGDQCAPQ 254
Query: 268 ALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQVKNLFIYSGETYSV 327
L V P KTYR+R++S T+L++L+F ++GH + VVEADG+Y+ PF ++ IYSGETYSV
Sbjct: 255 RLHVEPNKTYRIRLASSTALASLNFAVQGHKLVVVEADGNYITPFTTDDIDIYSGETYSV 314
Query: 328 LIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEPPAGPAWDDVKPRL 387
L+ DQDPS+NY+ V RKP TPP L + NY + P + PP P W+D
Sbjct: 315 LLTTDQDPSQNYYITAGVRGRKPNTPPALTVLNYVTAPSSQLPTSPPPETPRWNDFDRSK 374
Query: 388 AQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFNMPHTPYLISLKKN 447
S I A G +PP+T D+ ++ LNTQN + G +W++NNVS +P TPYL S+K N
Sbjct: 375 NFSKKIFAAMG-SPSPPETFDERLILLNTQNLIEGFTKWAINNVSLAVPGTPYLGSVKYN 434
Query: 448 LLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDIILQNANMMSPNNS 507
L F+++ PP Y +YDI P N A + FN TVD+ILQNAN ++ N S
Sbjct: 435 LRTGFNRSSPPKDYPV-DYDIMTPPRNRNAKQGNVSCVFPFNVTVDVILQNANGLNANAS 494
Query: 508 ETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPYGWTALRIVADNPG 567
E HPWHLHGHDFWVLGYGEGKF D YNL+NP ++NTV L+PYGWTALR V DNPG
Sbjct: 495 EIHPWHLHGHDFWVLGYGEGKFKPGVDEKTYNLKNPPLRNTVALYPYGWTALRFVTDNPG 554
Query: 568 VWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIMG 603
VW FHCHI+ H MGMGVVF EG+ ++GK+P +G
Sbjct: 555 VWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALG 556
BLAST of Sgr026112 vs. ExPASy Swiss-Prot
Match:
Q40588 (L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1)
HSP 1 Score: 560.5 bits (1443), Expect = 3.9e-158
Identity = 276/579 (47.67%), Postives = 365/579 (63.04%), Query Frame = 0
Query: 31 ARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDTVVVELKNSLGTENVAIH 90
A+TR++KW+V+Y SPD + + M ING+ PGPTI A+ GDTV V L N L TE V IH
Sbjct: 28 AKTRHFKWDVEYIHWSPDGEESVVMGINGQFPGPTIRAKAGDTVAVHLTNKLHTEGVVIH 87
Query: 91 WHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYGMQREDGLYGSIV 150
WHGIRQIGTPW+DGT ++QC I PGETF Y+F VD+ GTY YH HYGMQR GLYGS++
Sbjct: 88 WHGIRQIGTPWADGTAAISQCAINPGETFLYRFKVDKAGTYFYHGHYGMQRSAGLYGSLI 147
Query: 151 VSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGFTWVGEPDVRKLLPFPLF 210
V + EG+ EPF YD + +++L+DW+HK S E L++ N W+GEP
Sbjct: 148 VEVGEGEKEPFHYDGEFNLLLSDWWHKGSHEQEVDLSS--NPLRWIGEPQT--------- 207
Query: 211 IPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSLTSGLCNSSSPEC------- 270
+LL G +G+ SL + P+C
Sbjct: 208 ---------LLLNG-------------RGQYNC------SLAARFSKPPLPQCKLRGGEQ 267
Query: 271 -VPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQVKNLFIYSGE 330
P L V P K YRLRV+S T+L +LS I GH M VVEADG+YV+PF V+++ IYSGE
Sbjct: 268 YAPQILRVRPNKIYRLRVASTTALGSLSLAIGGHKMVVVEADGNYVQPFSVQDMDIYSGE 327
Query: 331 TYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEPPAGPAWDDV 390
+YSVL K DQDP++NYW + +V R+P TP GL + NY PN + P PP P W+D
Sbjct: 328 SYSVLFKTDQDPTKNYWISINVRGREPKTPQGLTLLNYLPNSASKFPTLPPPIAPLWNDY 387
Query: 391 KPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFNMPHTPYLIS 450
+ S+ I A G PP+ ++I+ LNTQN ++G+ +W++NNVS +P YL S
Sbjct: 388 NHSKSFSNKIFALMGSPKPPPQNHRRIIL-LNTQNKIDGYTKWAINNVSLVLPTQLYLGS 447
Query: 451 LKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDIILQNANMMS 510
++ ++ F PP + K+YD+ K APN +T + +Y L FN+T+DIILQNAN ++
Sbjct: 448 IRYG-INAFDTKPPPDNFP-KDYDVLKQAPNSNSTYGNGVYMLKFNTTIDIILQNANALA 507
Query: 511 PNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPYGWTALRIVA 570
+ SE HPWHLHGHDFWVLGYGEGKF +D K+NL+NP ++NT + P+GWTALR V
Sbjct: 508 KDVSEIHPWHLHGHDFWVLGYGEGKFS-EKDVKKFNLKNPPLRNTAVIFPFGWTALRFVT 563
Query: 571 DNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIM 602
DNPGVWAFHCHI+ H MGMGV+F EG+ V K+P +
Sbjct: 568 DNPGVWAFHCHIEPHLHMGMGVIFAEGVHLVKKIPKEAL 563
BLAST of Sgr026112 vs. ExPASy TrEMBL
Match:
A0A6J1BSL9 (L-ascorbate oxidase OS=Momordica charantia OX=3673 GN=LOC111005395 PE=3 SV=1)
HSP 1 Score: 1041.2 bits (2691), Expect = 2.8e-300
Identity = 500/604 (82.78%), Postives = 525/604 (86.92%), Query Frame = 0
Query: 1 MASLLQS--RRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTIN 60
MAS+LQS R ILL LLCV V +FL ESP AEART YYKWEV+ EFKSPDCYKKLAMTIN
Sbjct: 34 MASVLQSRGRGILLILLCVLV-IFLLESPAAEARTLYYKWEVRSEFKSPDCYKKLAMTIN 93
Query: 61 GRTPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGET 120
GRTPGPTIYA QGDT++V+LKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGET
Sbjct: 94 GRTPGPTIYAHQGDTIIVDLKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGET 153
Query: 121 FKYQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKS 180
FKYQFVVDRPGTYLYHAHYGMQRE+GLYGSI+V LPEGQSEPFSYDYDR+MI TDWYH S
Sbjct: 154 FKYQFVVDRPGTYLYHAHYGMQREEGLYGSIIVWLPEGQSEPFSYDYDRNMIFTDWYHNS 213
Query: 181 SQEHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRK 240
S EHAAGLA PGNGFTWVGEPD +L+ G +
Sbjct: 214 SHEHAAGLAAPGNGFTWVGEPDS------------------LLIQG-------------R 273
Query: 241 GEIQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIM 300
G +G SG CNSSSPEC PHAL VVPGKTYRLRVSSLTSLSALSF+IEGH M
Sbjct: 274 GRFNCSQLGSSPSESGRCNSSSPECFPHALAVVPGKTYRLRVSSLTSLSALSFEIEGHNM 333
Query: 301 TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIF 360
TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPAT PGLAIF
Sbjct: 334 TVVEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATAPGLAIF 393
Query: 361 NYYPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNT 420
NYYPNHQRR PPT P AGPAWDDVKPRLAQSHAIKAR+GFIHAPPKT D+VIVFLNTQNT
Sbjct: 394 NYYPNHQRRPPPTTPXAGPAWDDVKPRLAQSHAIKARRGFIHAPPKTTDRVIVFLNTQNT 453
Query: 421 VNGHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATV 480
V G RRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFK YDIF PAPNPEATV
Sbjct: 454 VKGRRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKGYDIFNPAPNPEATV 513
Query: 481 SDAIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYN 540
SDAIYRLDFN+TVD+ILQNAN M+ NNSETHPWHLHGHDFWVLGYGEGKFD+Y+DPS YN
Sbjct: 514 SDAIYRLDFNATVDVILQNANTMNSNNSETHPWHLHGHDFWVLGYGEGKFDVYKDPSGYN 573
Query: 541 LENPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPS 600
LENPIMKNTV +HP+GWTALR VADNPGVWAFHCHI+AHFFMGMGVVFEEGI KVG+LPS
Sbjct: 574 LENPIMKNTVAVHPFGWTALRFVADNPGVWAFHCHIEAHFFMGMGVVFEEGIHKVGRLPS 605
Query: 601 SIMG 603
SIMG
Sbjct: 634 SIMG 605
BLAST of Sgr026112 vs. ExPASy TrEMBL
Match:
A0A5A7SYW4 (L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G002620 PE=3 SV=1)
HSP 1 Score: 999.2 bits (2582), Expect = 1.2e-287
Identity = 485/602 (80.56%), Postives = 514/602 (85.38%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MASLLQ ++ L LL F+FL +S TAE+RT YKWEVK E KSPDC+KKL +TINGR
Sbjct: 1 MASLLQRKQYFLLLLS--SFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGR 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+
Sbjct: 61 SPGPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFR 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV +PEGQ+EPFSYD+DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSN 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHAAGLATPG+GFTWVGEPD +L+ G F SV S
Sbjct: 181 EHAAGLATPGSGFTWVGEPDS------------------LLIQGRGRFNCSVLGSS---- 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
SG CNSSS ECV + LTVVPGKTYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 241 ------------SGTCNSSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHSMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYW T+VVSR TPPGLAIFNY
Sbjct: 301 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQ + PPT PPA PAW+DV PRLAQS AIKARQGFIHAPPK ADKVIV LNTQNTVN
Sbjct: 361 YPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVN 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQ KPPTGYDFKNYDIF PAPNPEATVSD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY DPSKYNLE
Sbjct: 481 AIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLE 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI++VGKLP+SI
Sbjct: 541 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 566
Query: 601 MG 603
MG
Sbjct: 601 MG 566
BLAST of Sgr026112 vs. ExPASy TrEMBL
Match:
A0A1S3B151 (L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1)
HSP 1 Score: 999.2 bits (2582), Expect = 1.2e-287
Identity = 485/602 (80.56%), Postives = 514/602 (85.38%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MASLLQ ++ L LL F+FL +S TAE+RT YKWEVK E KSPDC+KKL +TINGR
Sbjct: 1 MASLLQRKQYFLLLLS--SFLFLLQSQTAESRTLNYKWEVKSELKSPDCFKKLTITINGR 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+
Sbjct: 61 SPGPTIYAHQGDTVIVELKNSLHTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFR 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV +PEGQ+EPFSYD+DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDHDRNLILTDWYHKSSN 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHAAGLATPG+GFTWVGEPD +L+ G F SV S
Sbjct: 181 EHAAGLATPGSGFTWVGEPDS------------------LLIQGRGRFNCSVLGSS---- 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
SG CNSSS ECV + LTVVPGKTYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 241 ------------SGTCNSSSTECVLYPLTVVPGKTYRLRVSSLTSLSALSFQIEGHSMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYW T+VVSR TPPGLAIFNY
Sbjct: 301 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQ + PPT PPA PAW+DV PRLAQS AIKARQGFIHAPPK ADKVIV LNTQNTVN
Sbjct: 361 YPNHQSKRPPTTPPAPPAWNDVPPRLAQSQAIKARQGFIHAPPKAADKVIVLLNTQNTVN 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQ KPPTGYDFKNYDIF PAPNPEATVSD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AI+RLDFNSTVD+ILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIY DPSKYNLE
Sbjct: 481 AIFRLDFNSTVDLILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYNDPSKYNLE 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI++VGKLP+SI
Sbjct: 541 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 566
Query: 601 MG 603
MG
Sbjct: 601 MG 566
BLAST of Sgr026112 vs. ExPASy TrEMBL
Match:
A0A0A0KK62 (L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1)
HSP 1 Score: 998.0 bits (2579), Expect = 2.7e-287
Identity = 487/602 (80.90%), Postives = 512/602 (85.05%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MAS LQS R LL LL FVF FL +S TAE+RT YKWEVK E KSPDC+KK+ +TINGR
Sbjct: 1 MASPLQSTRYLLILLS-FVF-FLLQSQTAESRTLNYKWEVKSELKSPDCFKKVTLTINGR 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGDTV+VELKNSL TENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETF+
Sbjct: 61 SPGPTIYAHQGDTVIVELKNSLFTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFR 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDRPGTYLYHAHYGMQREDGLYGSIVV +PEGQ+EPFSYD DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVWVPEGQTEPFSYDLDRNLILTDWYHKSSH 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHA GLATPG+GF WVGEPD +L+ G F S+ S
Sbjct: 181 EHATGLATPGSGFNWVGEPDS------------------LLIQGRGRFNCSILGSS---- 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
SG CNSSS ECV H LTVVPGKTYRLRVSSLTSLSALSFQIEGH MTV
Sbjct: 241 ------------SGTCNSSSTECVLHPLTVVPGKTYRLRVSSLTSLSALSFQIEGHTMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYW T+VVSR TPPGLAIFNY
Sbjct: 301 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWITTNVVSRSRTTPPGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNHQ R PPT PPA PAW+DV PRLAQS AIKARQGFIH PPK ADKVIV LNTQNTVN
Sbjct: 361 YPNHQSRLPPTTPPAPPAWNDVTPRLAQSQAIKARQGFIHTPPKAADKVIVLLNTQNTVN 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQ KPPTGYDFKNYDIF PAPNPEATVSD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQEKPPTGYDFKNYDIFNPAPNPEATVSD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFD+Y+DPSKYNLE
Sbjct: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDLYKDPSKYNLE 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTVPLHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI++VGKLP+SI
Sbjct: 541 NPIMKNTVPLHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIERVGKLPTSI 566
Query: 601 MG 603
MG
Sbjct: 601 MG 566
BLAST of Sgr026112 vs. ExPASy TrEMBL
Match:
A0A6J1GE53 (L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1)
HSP 1 Score: 982.6 bits (2539), Expect = 1.2e-282
Identity = 471/602 (78.24%), Postives = 506/602 (84.05%), Query Frame = 0
Query: 1 MASLLQSRRILLSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGR 60
MA LLQS + LL L C FVFL S AE+RT +YKWEVKYE +SPDC+KKL +TING
Sbjct: 1 MAPLLQSTQYLLILFC--FFVFLSASLVAESRTLFYKWEVKYEIRSPDCFKKLTITINGG 60
Query: 61 TPGPTIYAQQGDTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFK 120
+PGPTIYA QGD V+VELKNSL +ENVA+HWHGIRQIGTPWSDGTEGVTQCP+LPGETFK
Sbjct: 61 SPGPTIYAHQGDNVIVELKNSLASENVAVHWHGIRQIGTPWSDGTEGVTQCPVLPGETFK 120
Query: 121 YQFVVDRPGTYLYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQ 180
YQFVVDR GTYLYHAHYGMQR+DGLYGSIVV LPEGQSEPFSYD+DR++ILTDWYHKSS
Sbjct: 121 YQFVVDRAGTYLYHAHYGMQRQDGLYGSIVVWLPEGQSEPFSYDHDRNLILTDWYHKSSH 180
Query: 181 EHAAGLATPGNGFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGE 240
EHA GLATP GF WVGEPD +L+ G +G
Sbjct: 181 EHATGLATPDPGFQWVGEPDS------------------LLIQG-------------RGR 240
Query: 241 IQLLSVGFKSLTSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTV 300
+ +G L S CNSSSP+CV + LTVVPGKTYRLRVSSLTSLSALSFQIE H MTV
Sbjct: 241 FKCSLLGSSGLASATCNSSSPQCVAYPLTVVPGKTYRLRVSSLTSLSALSFQIESHTMTV 300
Query: 301 VEADGHYVEPFQVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNY 360
VEADGHYVEPFQV NLFIYSGETYSVLIKADQDPSRNYWA T+VVSR TPPGLAIFNY
Sbjct: 301 VEADGHYVEPFQVNNLFIYSGETYSVLIKADQDPSRNYWATTNVVSRPRTTPPGLAIFNY 360
Query: 361 YPNHQRRSPPTEPPAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVN 420
YPNH+R+ PPT PPAGPAW+DV PRLAQSHAIKARQGFIH PPK ADKVIV LNTQNTV
Sbjct: 361 YPNHRRKLPPTTPPAGPAWNDVAPRLAQSHAIKARQGFIHPPPKIADKVIVLLNTQNTVK 420
Query: 421 GHRRWSVNNVSFNMPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSD 480
G RRWS+NNVSFNMPHTPYLI+LK NLLHTFSQAKPPTGYDFKNYDIF PAPN EAT SD
Sbjct: 421 GRRRWSLNNVSFNMPHTPYLIALKHNLLHTFSQAKPPTGYDFKNYDIFNPAPNQEATESD 480
Query: 481 AIYRLDFNSTVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLE 540
AI+RL+F STVDIILQNANMMSPN+S+THPWHLHGHDFWVLGYGEGKFDIY+DPSKYNLE
Sbjct: 481 AIFRLEFKSTVDIILQNANMMSPNSSDTHPWHLHGHDFWVLGYGEGKFDIYRDPSKYNLE 540
Query: 541 NPIMKNTVPLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSI 600
NPIMKNTV LHPYGWTALR VADNPGVWAFHCHIDAHFFMGMGVVFEEGI +VG LP+SI
Sbjct: 541 NPIMKNTVALHPYGWTALRFVADNPGVWAFHCHIDAHFFMGMGVVFEEGIDQVGNLPTSI 569
Query: 601 MG 603
MG
Sbjct: 601 MG 569
BLAST of Sgr026112 vs. TAIR 10
Match:
AT4G39830.1 (Cupredoxin superfamily protein )
HSP 1 Score: 772.3 bits (1993), Expect = 4.6e-223
Identity = 378/594 (63.64%), Postives = 440/594 (74.07%), Query Frame = 0
Query: 12 LSLLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQG 71
L +LC F+ +F F S + + R +KWEVKYEFKSPDC++KL +TING+ PGPTI AQQG
Sbjct: 16 LMVLC-FIALF-FSSVLCQGKIRRFKWEVKYEFKSPDCFEKLVITINGKFPGPTIKAQQG 75
Query: 72 DTVVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTY 131
DT+VVELKNS TENVA+HWHGIRQIGTPW DG EGVTQCPILPGE F YQFVVDRPGTY
Sbjct: 76 DTIVVELKNSFMTENVAVHWHGIRQIGTPWFDGVEGVTQCPILPGEVFIYQFVVDRPGTY 135
Query: 132 LYHAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGN 191
+YH+HYGMQRE GL G I VS P + EPF+YDYDR+ +LTDWYHKS E A GLA+
Sbjct: 136 MYHSHYGMQRESGLIGMIQVSPPATEPEPFTYDYDRNFLLTDWYHKSMSEKATGLAS--I 195
Query: 192 GFTWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSL 251
F WVGEP +++ G F S + SL
Sbjct: 196 PFKWVGEPQS------------------LMIQGRGRFNCSNNLTTP-----------PSL 255
Query: 252 TSGLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPF 311
SG+CN S+ +C LTV+PGKTYRLR+ SLT+LSALSFQIEGH +TVVEADGHYVEPF
Sbjct: 256 VSGVCNVSNADCSRFILTVIPGKTYRLRIGSLTALSALSFQIEGHNLTVVEADGHYVEPF 315
Query: 312 QVKNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPT 371
VKNLF+YSGETYSVL+KADQ+P RNYW + +VSR TPP A+ NYYPNH RR PPT
Sbjct: 316 TVKNLFVYSGETYSVLLKADQNPRRNYWITSSIVSRPATTPPATAVLNYYPNHPRRRPPT 375
Query: 372 EPPAG--PAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNN 431
+ P W+D + RLAQS AIKAR+GFIHA P+ +DKVIV LNTQN VNG+RRWSVNN
Sbjct: 376 SESSNIVPEWNDTRSRLAQSLAIKARRGFIHALPENSDKVIVLLNTQNEVNGYRRWSVNN 435
Query: 432 VSFNMPHTPYLISLKKNLLHTFS-QAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFN 491
VS++ P TPYLI+LK+NL + F + P YD +NYDIF N AT SD IYRL FN
Sbjct: 436 VSYHHPKTPYLIALKQNLTNAFDWRFTAPENYDSRNYDIFAKPLNANATTSDGIYRLRFN 495
Query: 492 STVDIILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTV 551
STVD+ILQNAN M+ NNSETHPWHLHGHDFWVLGYGEGKF+ +DP +YN +PI KNTV
Sbjct: 496 STVDVILQNANTMNANNSETHPWHLHGHDFWVLGYGEGKFNESEDPKRYNRVDPIKKNTV 555
Query: 552 PLHPYGWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIMG 603
+ P+GWTALR ADNPGVW+FHCHI++HFFMGMG+VFE GI KV LPSSIMG
Sbjct: 556 AVQPFGWTALRFRADNPGVWSFHCHIESHFFMGMGIVFESGIDKVSSLPSSIMG 576
BLAST of Sgr026112 vs. TAIR 10
Match:
AT5G21105.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 548.9 bits (1413), Expect = 8.3e-156
Identity = 273/586 (46.59%), Postives = 360/586 (61.43%), Query Frame = 0
Query: 19 VFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDTVVVEL 78
V V + TA A R Y WEV+Y++ SPDC + MT+NG PGPTI A GDT+VV L
Sbjct: 23 VLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNL 82
Query: 79 KNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYG 138
N L TE + IHWHGIRQ G+PW+DG GVTQC I PGETF Y F V++PGT+ YH HYG
Sbjct: 83 TNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYG 142
Query: 139 MQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGFTWVGE 198
MQR GLYGS++V + +G+SE YD + +++L+DW+H++ GL++ W+GE
Sbjct: 143 MQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGE 202
Query: 199 PDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSLTS-GLCN 258
+L+ G F S L+ F + TS +C
Sbjct: 203 AQS------------------ILINGRGQFNCS------------LAAQFSNNTSLPMCT 262
Query: 259 -SSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQVKNL 318
+C P L V P KTYR+R+SS T+L++L+ ++GH + VVEADG+Y+ PF ++
Sbjct: 263 FKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDI 322
Query: 319 FIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEPPAG 378
IYSGE+YSVL+ DQDPS+NY+ + V RKP T L I NY + P + PP
Sbjct: 323 DIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVT 382
Query: 379 PAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFNMPH 438
P WDD + S I + G +PPK K ++ LNTQN ++G+ +W++NNVS P
Sbjct: 383 PRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPA 442
Query: 439 TPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDIILQ 498
TPYL S+K NL F++ PP Y +YDI P P P T + IY FN TVD+I+Q
Sbjct: 443 TPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQ 502
Query: 499 NANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPYGWT 558
NAN++ SE HPWHLHGHDFWVLGYG+GKF D YNL+NP ++NT L+PYGWT
Sbjct: 503 NANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWT 562
Query: 559 ALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIMG 603
A+R V DNPGVW FHCHI+ H MGMGVVF EG+ ++GK+P +G
Sbjct: 563 AIRFVTDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALG 574
BLAST of Sgr026112 vs. TAIR 10
Match:
AT5G21100.1 (Plant L-ascorbate oxidase )
HSP 1 Score: 524.2 bits (1349), Expect = 2.2e-148
Identity = 268/591 (45.35%), Postives = 354/591 (59.90%), Query Frame = 0
Query: 14 LLCVFVFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDT 73
LL V V F +A A WEV+Y++ PDC + + M ING+ PGPTI A GDT
Sbjct: 8 LLTVVVVAF----HSASAAVVESTWEVEYKYWWPDCKEGIVMAINGQFPGPTIDAVAGDT 67
Query: 74 VVVELKNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLY 133
V++ + N L TE V IHWHGIRQ GTPW+DG GVTQCPI PGETF Y+F+VD+ GT+ Y
Sbjct: 68 VIIHVVNKLSTEGVVIHWHGIRQKGTPWADGAAGVTQCPINPGETFTYKFIVDKAGTHFY 127
Query: 134 HAHYGMQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGF 193
H HYGMQR GLYG ++V P+ E YD + +++L+DW+H+S HA LA
Sbjct: 128 HGHYGMQRSSGLYGMLIVRSPK---ERLIYDGEFNLLLSDWWHQSI--HAQELALSSRPM 187
Query: 194 TWVGEPDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSLTS 253
W+GEP +L+ G F S KG G K + +
Sbjct: 188 RWIGEPQS------------------LLINGRGQFNCSQAAYFNKG-------GEKDVCT 247
Query: 254 GLCNSSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQV 313
+ +C P L V P + YRLR++S T+L++L+ ++GH + VVEADG+YV PF V
Sbjct: 248 ---FKENDQCAPQTLRVEPNRVYRLRIASTTALASLNLAVQGHQLVVVEADGNYVAPFTV 307
Query: 314 KNLFIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEP 373
++ +YSGETYSVL+K + PS+ YW + V R+P TP L + NY + R P P
Sbjct: 308 NDIDVYSGETYSVLLKTNALPSKKYWISVGVRGREPKTPQALTVINYVDATESR-PSHPP 367
Query: 374 PAGPAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFN 433
P P W+D + S I A +G+ P K+ D++I+ LNTQN + +WS+NNVS +
Sbjct: 368 PVTPIWNDTDRSKSFSKKIFAAKGYPKPPEKSHDQLIL-LNTQNLYEDYTKWSINNVSLS 427
Query: 434 MPHTPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDI 493
+P TPYL S++ L + P NYDI KP PNP T IY F VD+
Sbjct: 428 VPVTPYLGSIRYGLKSAYDLKSPAKKLIMDNYDIMKPPPNPNTTKGSGIYNFAFGIVVDV 487
Query: 494 ILQNANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPY 553
ILQNAN++ SE HPWH+HGHDFWVLGYGEGKF D +NL+NP ++NTV L+P+
Sbjct: 488 ILQNANVLKGVISEIHPWHIHGHDFWVLGYGEGKFKPGIDEKTFNLKNPPLRNTVVLYPF 547
Query: 554 GWTALRIVADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGK--LPSSIMG 603
GWTA+R V DNPGVW FHCHI+ H MGMGVVF EG+ ++GK +P +G
Sbjct: 548 GWTAIRFVTDNPGVWFFHCHIEPHLHMGMGVVFVEGVDRIGKMEIPDEALG 559
BLAST of Sgr026112 vs. TAIR 10
Match:
AT5G21105.3 (Plant L-ascorbate oxidase )
HSP 1 Score: 496.5 bits (1277), Expect = 4.9e-140
Identity = 252/550 (45.82%), Postives = 333/550 (60.55%), Query Frame = 0
Query: 19 VFVFLFESPTAEARTRYYKWEVKYEFKSPDCYKKLAMTINGRTPGPTIYAQQGDTVVVEL 78
V V + TA A R Y WEV+Y++ SPDC + MT+NG PGPTI A GDT+VV L
Sbjct: 23 VLVVAVLTHTASAAVREYHWEVEYKYWSPDCKEGAVMTVNGEFPGPTIKAFAGDTIVVNL 82
Query: 79 KNSLGTENVAIHWHGIRQIGTPWSDGTEGVTQCPILPGETFKYQFVVDRPGTYLYHAHYG 138
N L TE + IHWHGIRQ G+PW+DG GVTQC I PGETF Y F V++PGT+ YH HYG
Sbjct: 83 TNKLTTEGLVIHWHGIRQFGSPWADGAAGVTQCAINPGETFTYNFTVEKPGTHFYHGHYG 142
Query: 139 MQREDGLYGSIVVSLPEGQSEPFSYDYDRSMILTDWYHKSSQEHAAGLATPGNGFTWVGE 198
MQR GLYGS++V + +G+SE YD + +++L+DW+H++ GL++ W+GE
Sbjct: 143 MQRSAGLYGSLIVDVAKGKSERLRYDGEFNLLLSDWWHEAIPSQELGLSS--KPMRWIGE 202
Query: 199 PDVRKLLPFPLFIPAIKHMDWVLLMGFFLFILSVTFDSRKGEIQLLSVGFKSLTS-GLCN 258
+L+ G F S L+ F + TS +C
Sbjct: 203 AQS------------------ILINGRGQFNCS------------LAAQFSNNTSLPMCT 262
Query: 259 -SSSPECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQVKNL 318
+C P L V P KTYR+R+SS T+L++L+ ++GH + VVEADG+Y+ PF ++
Sbjct: 263 FKEGDQCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDI 322
Query: 319 FIYSGETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEPPAG 378
IYSGE+YSVL+ DQDPS+NY+ + V RKP T L I NY + P + PP
Sbjct: 323 DIYSGESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVT 382
Query: 379 PAWDDVKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFNMPH 438
P WDD + S I + G +PPK K ++ LNTQN ++G+ +W++NNVS P
Sbjct: 383 PRWDDFERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPA 442
Query: 439 TPYLISLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDIILQ 498
TPYL S+K NL F++ PP Y +YDI P P P T + IY FN TVD+I+Q
Sbjct: 443 TPYLGSVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQ 502
Query: 499 NANMMSPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPYGWT 558
NAN++ SE HPWHLHGHDFWVLGYG+GKF D YNL+NP ++NT L+PYGWT
Sbjct: 503 NANVLKGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWT 538
Query: 559 ALRIVADNPG 567
A+R V DNPG
Sbjct: 563 AIRFVTDNPG 538
BLAST of Sgr026112 vs. TAIR 10
Match:
AT5G21105.2 (Plant L-ascorbate oxidase )
HSP 1 Score: 362.1 bits (928), Expect = 1.4e-99
Identity = 171/341 (50.15%), Postives = 224/341 (65.69%), Query Frame = 0
Query: 262 ECVPHALTVVPGKTYRLRVSSLTSLSALSFQIEGHIMTVVEADGHYVEPFQVKNLFIYSG 321
+C P L V P KTYR+R+SS T+L++L+ ++GH + VVEADG+Y+ PF ++ IYSG
Sbjct: 45 QCAPQILHVEPNKTYRIRLSSTTALASLNLAVQGHKLVVVEADGNYITPFTTDDIDIYSG 104
Query: 322 ETYSVLIKADQDPSRNYWAATHVVSRKPATPPGLAIFNYYPNHQRRSPPTEPPAGPAWDD 381
E+YSVL+ DQDPS+NY+ + V RKP T L I NY + P + PP P WDD
Sbjct: 105 ESYSVLLTTDQDPSQNYYISVGVRGRKPNTTQALTILNYVTAPASKLPSSPPPVTPRWDD 164
Query: 382 VKPRLAQSHAIKARQGFIHAPPKTADKVIVFLNTQNTVNGHRRWSVNNVSFNMPHTPYLI 441
+ S I + G +PPK K ++ LNTQN ++G+ +W++NNVS P TPYL
Sbjct: 165 FERSKNFSKKIFSAMG-SPSPPKKYRKRLILLNTQNLIDGYTKWAINNVSLVTPATPYLG 224
Query: 442 SLKKNLLHTFSQAKPPTGYDFKNYDIFKPAPNPEATVSDAIYRLDFNSTVDIILQNANMM 501
S+K NL F++ PP Y +YDI P P P T + IY FN TVD+I+QNAN++
Sbjct: 225 SVKYNLKLGFNRKSPPRSYRM-DYDIMNPPPFPNTTTGNGIYVFPFNVTVDVIIQNANVL 284
Query: 502 SPNNSETHPWHLHGHDFWVLGYGEGKFDIYQDPSKYNLENPIMKNTVPLHPYGWTALRIV 561
SE HPWHLHGHDFWVLGYG+GKF D YNL+NP ++NT L+PYGWTA+R V
Sbjct: 285 KGIVSEIHPWHLHGHDFWVLGYGDGKFKPGIDEKTYNLKNPPLRNTAILYPYGWTAIRFV 344
Query: 562 ADNPGVWAFHCHIDAHFFMGMGVVFEEGIQKVGKLPSSIMG 603
DNPGVW FHCHI+ H MGMGVVF EG+ ++GK+P +G
Sbjct: 345 TDNPGVWFFHCHIEPHLHMGMGVVFAEGLNRIGKVPDEALG 383
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022132566.1 | 5.8e-300 | 82.78 | L-ascorbate oxidase [Momordica charantia] | [more] |
| XP_038881120.1 | 9.2e-290 | 80.90 | L-ascorbate oxidase [Benincasa hispida] | [more] |
| XP_008440636.1 | 2.5e-287 | 80.56 | PREDICTED: L-ascorbate oxidase [Cucumis melo] >KAA0036280.1 L-ascorbate oxidase ... | [more] |
| XP_004143482.1 | 5.6e-287 | 80.90 | L-ascorbate oxidase [Cucumis sativus] >KGN48772.1 hypothetical protein Csa_00307... | [more] |
| KAG6594863.1 | 1.1e-282 | 78.74 | L-ascorbate oxidase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7026826... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| P14133 | 4.8e-164 | 47.92 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 PE=1 SV=1 | [more] |
| P24792 | 2.0e-162 | 46.93 | L-ascorbate oxidase OS=Cucurbita maxima OX=3661 GN=AAO PE=1 SV=2 | [more] |
| P37064 | 3.4e-162 | 48.34 | L-ascorbate oxidase OS=Cucurbita pepo var. melopepo OX=3665 PE=1 SV=1 | [more] |
| M4DUF2 | 1.8e-158 | 47.40 | L-ascorbate oxidase OS=Brassica rapa subsp. pekinensis OX=51351 GN=AO PE=2 SV=1 | [more] |
| Q40588 | 3.9e-158 | 47.67 | L-ascorbate oxidase OS=Nicotiana tabacum OX=4097 GN=AAO PE=2 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1BSL9 | 2.8e-300 | 82.78 | L-ascorbate oxidase OS=Momordica charantia OX=3673 GN=LOC111005395 PE=3 SV=1 | [more] |
| A0A5A7SYW4 | 1.2e-287 | 80.56 | L-ascorbate oxidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G... | [more] |
| A0A1S3B151 | 1.2e-287 | 80.56 | L-ascorbate oxidase OS=Cucumis melo OX=3656 GN=LOC103484994 PE=3 SV=1 | [more] |
| A0A0A0KK62 | 2.7e-287 | 80.90 | L-ascorbate oxidase OS=Cucumis sativus OX=3659 GN=Csa_6G500640 PE=3 SV=1 | [more] |
| A0A6J1GE53 | 1.2e-282 | 78.24 | L-ascorbate oxidase OS=Cucurbita moschata OX=3662 GN=LOC111453168 PE=3 SV=1 | [more] |
