Sgr025889 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025889 vs. NCBI nr
Match: XP_022141422.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 1478.8 bits (3827), Expect = 0.0e+00
Identity = 747/783 (95.40%), Postives = 762/783 (97.32%), Query Frame = 0

Query: 204 NVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARRR 263
           +VSTFRP VAFRLGSFPR W LFNRNC+ R CPK CISVTNVVYAEDVMVPG  KSARRR
Sbjct: 9   DVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPGTAKSARRR 68

Query: 264 EQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR 323
           EQIELE DSIS+LNER+RRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR
Sbjct: 69  EQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR 128

Query: 324 KESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAKL 383
            ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVF+EYADGMAKL
Sbjct: 129 NESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIEYADGMAKL 188

Query: 384 PVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK 443
           PVKQASRMLYRYNLPNETKRPR LSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK
Sbjct: 189 PVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK 248

Query: 444 QRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTEV 503
           QRRPPYPKC AM+DFAAQFPYEPTVDQK+AFSDV+KDLTERETPMDRLICGDVGFGKTEV
Sbjct: 249 QRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGDVGFGKTEV 308

Query: 504 ALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL 563
           ALRAIFCVVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL
Sbjct: 309 ALRAIFCVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL 368

Query: 564 DMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA 623
           DMIK GQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA
Sbjct: 369 DMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA 428

Query: 624 TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYVL 683
           TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKV SAIKYELERGGQVFYVL
Sbjct: 429 TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQVFYVL 488

Query: 684 PRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD 743
           PRIKGLEEVKDFLE SFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD
Sbjct: 489 PRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD 548

Query: 744 IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC 803
           IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC
Sbjct: 549 IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC 608

Query: 804 RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVPY 863
           RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEH VVSVPY
Sbjct: 609 RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVPY 668

Query: 864 QSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYSM 923
           QSVKI +DI PHLPSEYINYLENPMKIINGAEKAAETDIW LMQFTENLRRQHGKEPYSM
Sbjct: 669 QSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQHGKEPYSM 728

Query: 924 EILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEEH 983
           EILLKKLYVRRMAADLGIS+IY SGKTVCMET+MN+KVFKLITDSMTSEVH+NCLSFEEH
Sbjct: 729 EILLKKLYVRRMAADLGISRIYASGKTVCMETKMNRKVFKLITDSMTSEVHQNCLSFEEH 788

Query: 984 QIK 987
            IK
Sbjct: 789 HIK 791

BLAST of Sgr025889 vs. NCBI nr
Match: XP_038907244.1 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 1431.4 bits (3704), Expect = 0.0e+00
Identity = 722/783 (92.21%), Postives = 755/783 (96.42%), Query Frame = 0

Query: 204 NVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARRR 263
           +VS+ RP VAF+LGSFP L GLFNRN +FRH PK  +S+TNV+YAEDV+VPG  KSARRR
Sbjct: 10  DVSSSRPLVAFKLGSFPSLRGLFNRNSTFRHFPKRYVSITNVIYAEDVIVPGIAKSARRR 69

Query: 264 EQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR 323
           EQIELE DSIS+LNERIRR+HGKRESSRT MD+EEADRYIQMVKEQQQRGLQKLKGDRQR
Sbjct: 70  EQIELERDSISILNERIRRFHGKRESSRTVMDSEEADRYIQMVKEQQQRGLQKLKGDRQR 129

Query: 324 KESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAKL 383
           K+SDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVF+EYADGMAKL
Sbjct: 130 KDSDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIEYADGMAKL 189

Query: 384 PVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK 443
           PVKQASRMLYRYNLPNE KRPR LSKLNDT+TWEKRKTKGKVAIQKMVVDLMELYLHRLK
Sbjct: 190 PVKQASRMLYRYNLPNENKRPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLMELYLHRLK 249

Query: 444 QRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTEV 503
           QRR PYPKC AM++F+AQFPYEPTVDQK+AF DV++DLT+RETPMDRLICGDVGFGKTEV
Sbjct: 250 QRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTQRETPMDRLICGDVGFGKTEV 309

Query: 504 ALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL 563
           ALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFS FPDVQIGLLSRFQTKAEKEKHL
Sbjct: 310 ALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSLFPDVQIGLLSRFQTKAEKEKHL 369

Query: 564 DMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA 623
           +MIK GQL+IIVGTHSLLGDRVVY+ LGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA
Sbjct: 370 EMIKEGQLNIIVGTHSLLGDRVVYSKLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA 429

Query: 624 TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYVL 683
           TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKV SAI+YELERGGQVFYVL
Sbjct: 430 TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIRYELERGGQVFYVL 489

Query: 684 PRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD 743
           PRIKGLEEVK+FLE SFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD
Sbjct: 490 PRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD 549

Query: 744 IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC 803
           IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLS++ALERLAALEEC
Sbjct: 550 IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSEEALERLAALEEC 609

Query: 804 RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVPY 863
           RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLSKVDEH VVSVPY
Sbjct: 610 RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLSKVDEHRVVSVPY 669

Query: 864 QSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYSM 923
           QSVKI IDINPHLPSEYINYLENPMKI+NGAE AAETDIWTLMQFTENLRRQHGKEPYSM
Sbjct: 670 QSVKIDIDINPHLPSEYINYLENPMKILNGAESAAETDIWTLMQFTENLRRQHGKEPYSM 729

Query: 924 EILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEEH 983
           EILLKKLYVRRMAADLGIS+IY SGK VCMET MNKKVFKLI+DSMTSEVHRNCLSFEEH
Sbjct: 730 EILLKKLYVRRMAADLGISRIYASGKAVCMETNMNKKVFKLISDSMTSEVHRNCLSFEEH 789

Query: 984 QIK 987
           QIK
Sbjct: 790 QIK 792

BLAST of Sgr025889 vs. NCBI nr
Match: KAA0066106.1 (ATP-dependent DNA helicase [Cucumis melo var. makuwa] >TYK15145.1 ATP-dependent DNA helicase [Cucumis melo var. makuwa])

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 719/785 (91.59%), Postives = 754/785 (96.05%), Query Frame = 0

Query: 204 NVSTFRPPVAFRLGSFPRL--WGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSAR 263
           +VS+ RP +AF+L SFP L  WGLFNRNC+FRH P  C+SVTNV YAEDV+VPG  KSAR
Sbjct: 10  DVSSSRPLLAFKLRSFPTLWGWGLFNRNCTFRHFPNRCVSVTNVAYAEDVIVPGTAKSAR 69

Query: 264 RREQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDR 323
           RR+QIELE DSISVLNERI R+HGKR+SSRT MD+EEADRYIQMVKEQQQRGLQKLKGD+
Sbjct: 70  RRDQIELERDSISVLNERILRFHGKRDSSRTTMDSEEADRYIQMVKEQQQRGLQKLKGDK 129

Query: 324 QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMA 383
           QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFV IKFDVQKGSTEP EYVF+EYADGMA
Sbjct: 130 QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVRIKFDVQKGSTEPTEYVFIEYADGMA 189

Query: 384 KLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHR 443
           KLPVKQASRMLYRYNLPNE K+PR LSKLNDT+TWEKRKTKGKVAIQKMVVDLMELYLHR
Sbjct: 190 KLPVKQASRMLYRYNLPNENKKPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLMELYLHR 249

Query: 444 LKQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKT 503
           LKQRR PYPKC AM++F+AQFPYEPTVDQK+AF DV++DLTERETPMDRLICGDVGFGKT
Sbjct: 250 LKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTERETPMDRLICGDVGFGKT 309

Query: 504 EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK 563
           EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK
Sbjct: 310 EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK 369

Query: 564 HLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTL 623
           HL+MIK GQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS+DVLTL
Sbjct: 370 HLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSVDVLTL 429

Query: 624 SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFY 683
           SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKV SAIKYELERGGQ+FY
Sbjct: 430 SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQIFY 489

Query: 684 VLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG 743
           VLPRIKGLEEVK+FLE SFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG
Sbjct: 490 VLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG 549

Query: 744 LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALE 803
           LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD+ALERLAALE
Sbjct: 550 LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDEALERLAALE 609

Query: 804 ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSV 863
           ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEH VVSV
Sbjct: 610 ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSV 669

Query: 864 PYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPY 923
           PYQSVKI IDINPHLPSEYINYLENPMKI+NGAE+AAETDIWTLMQFTENLRR HGKEPY
Sbjct: 670 PYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRHHGKEPY 729

Query: 924 SMEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFE 983
           SMEILLKKLYVRRMAADLGIS+IY SGK VCMET MNKKVF+LI+DSMTSEVHRNCLSFE
Sbjct: 730 SMEILLKKLYVRRMAADLGISRIYASGKVVCMETNMNKKVFQLISDSMTSEVHRNCLSFE 789

Query: 984 EHQIK 987
           EHQIK
Sbjct: 790 EHQIK 794

BLAST of Sgr025889 vs. NCBI nr
Match: XP_023529599.1 (ATP-dependent DNA helicase At3g02060, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1425.2 bits (3688), Expect = 0.0e+00
Identity = 718/782 (91.82%), Postives = 752/782 (96.16%), Query Frame = 0

Query: 205 VSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARRRE 264
           VS+ RP VAFRLGSFP+LWGLFNRNC+ RHC K C+S+TNVVYAEDVMVPGA KSARRRE
Sbjct: 10  VSSSRPLVAFRLGSFPKLWGLFNRNCNLRHCSKRCVSITNVVYAEDVMVPGAAKSARRRE 69

Query: 265 QIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQRK 324
           QIELE DSI+VLNERIRR+H KRESS+TAM+AEEADRYIQMVKEQQQRGLQKLKG+RQ+K
Sbjct: 70  QIELEHDSITVLNERIRRFHVKRESSKTAMEAEEADRYIQMVKEQQQRGLQKLKGNRQKK 129

Query: 325 ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAKLP 384
           ESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVF+EYADGMAKL 
Sbjct: 130 ESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIEYADGMAKLS 189

Query: 385 VKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLKQ 444
           VKQASRMLYRY+LPNE KRPR LSKLNDT+TWEKRKTKGK+AIQKMVVDLMELYLHRLKQ
Sbjct: 190 VKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLMELYLHRLKQ 249

Query: 445 RRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTEVA 504
           RRPPYPKC AM++FAAQF Y PTVDQK+AF DV+KDLTERETPMDRLICGDVGFGKTEVA
Sbjct: 250 RRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGDVGFGKTEVA 309

Query: 505 LRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHLD 564
           LRAIF VVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTK+EK+KHL+
Sbjct: 310 LRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKSEKDKHLE 369

Query: 565 MIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSAT 624
           MIK GQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSAT
Sbjct: 370 MIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSAT 429

Query: 625 PIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYVLP 684
           PIPRTLYLALTGFRDASLITTPPPERVPIKTHLS FSKEKV SAIKYELERGGQVFYV P
Sbjct: 430 PIPRTLYLALTGFRDASLITTPPPERVPIKTHLSAFSKEKVKSAIKYELERGGQVFYVFP 489

Query: 685 RIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLDI 744
           RIKGLEEVKDFLE SFPDIEI LAHGKQYSKQLEETMENFALG IKILICTNIVESGLDI
Sbjct: 490 RIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFALGAIKILICTNIVESGLDI 549

Query: 745 QNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEECR 804
           QNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALERLAALEECR
Sbjct: 550 QNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALERLAALEECR 609

Query: 805 ELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVPYQ 864
           ELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDEH VVSVPYQ
Sbjct: 610 ELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVPYQ 669

Query: 865 SVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYSME 924
           SVKI IDINPHLPSEYINYLENPMKIINGAEKAAETDIW LMQFTENLRRQHGKEPYSME
Sbjct: 670 SVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQHGKEPYSME 729

Query: 925 ILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEEHQ 984
           ILLKKLYVRRMAADLGI +IY SGKTVCMET+MNKKVFKLI++SM SEVHRNCL+FEEHQ
Sbjct: 730 ILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHRNCLTFEEHQ 789

Query: 985 IK 987
           IK
Sbjct: 790 IK 791

BLAST of Sgr025889 vs. NCBI nr
Match: XP_022957218.1 (ATP-dependent DNA helicase At3g02060, chloroplastic [Cucurbita moschata] >XP_022957227.1 ATP-dependent DNA helicase At3g02060, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 718/784 (91.58%), Postives = 754/784 (96.17%), Query Frame = 0

Query: 203 SNVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARR 262
           ++VS+ RP VAFRLGSFP+LWGLFNRNC+ RH  K C+S+TNVVYAEDVMVPGA KSARR
Sbjct: 8   ADVSSSRPLVAFRLGSFPKLWGLFNRNCNLRHFSKRCVSITNVVYAEDVMVPGAAKSARR 67

Query: 263 REQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQ 322
           REQIELE DSI+VLNERIRR+H KRESS+TAMDAEEADRYIQMVKEQQQRGLQKLKG+RQ
Sbjct: 68  REQIELEHDSITVLNERIRRFHVKRESSKTAMDAEEADRYIQMVKEQQQRGLQKLKGNRQ 127

Query: 323 RKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAK 382
           +KESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVF+EYADGMAK
Sbjct: 128 KKESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIEYADGMAK 187

Query: 383 LPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRL 442
           L VKQASRMLYRY+LPNE KRPR LSKLNDT+TWEKRKTKGK+AIQKMVVDLMELYLHRL
Sbjct: 188 LSVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLMELYLHRL 247

Query: 443 KQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTE 502
           KQRRPPYPKC AM++FAAQF Y PTVDQK+AF DV+KDLTERETPMDRLICGDVGFGKTE
Sbjct: 248 KQRRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGDVGFGKTE 307

Query: 503 VALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKH 562
           VALRAIF VVSAGKQAMVLAPTIVLAKQHFEVIT RFSSFPDVQIGLLSRFQTK+EK+KH
Sbjct: 308 VALRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITHRFSSFPDVQIGLLSRFQTKSEKDKH 367

Query: 563 LDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS 622
           L+MIK GQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS
Sbjct: 368 LEMIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS 427

Query: 623 ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYV 682
           ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKVNSAIKYELERGGQVFYV
Sbjct: 428 ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVNSAIKYELERGGQVFYV 487

Query: 683 LPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGL 742
            PRIKGLEEVKDFLE SFPDIEI LAHGKQYSKQLEETMENFALG IKILICTNIVESGL
Sbjct: 488 FPRIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFALGAIKILICTNIVESGL 547

Query: 743 DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEE 802
           DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALERLAALEE
Sbjct: 548 DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALERLAALEE 607

Query: 803 CRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVP 862
           CRELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDEH VVSVP
Sbjct: 608 CRELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVP 667

Query: 863 YQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYS 922
           YQSVKI IDINPHLPSEYINYLENPMKIINGAEKAAETDIW LMQFTENLRRQHGKEPYS
Sbjct: 668 YQSVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQHGKEPYS 727

Query: 923 MEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEE 982
           MEILLKKLYVRRMAADLGI +IY SGKTVCMET+MNKKVFKLI++SM SEVHRNCL+FEE
Sbjct: 728 MEILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHRNCLTFEE 787

Query: 983 HQIK 987
           HQIK
Sbjct: 788 HQIK 791

BLAST of Sgr025889 vs. ExPASy Swiss-Prot
Match: F4JFJ3 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g02060 PE=2 SV=1)

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 616/788 (78.17%), Postives = 691/788 (87.69%), Query Frame = 0

Query: 202 LSNVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSAR 261
           L N      P+  +L SFP    LF  +   R   +   S+  +V A   +   A K  R
Sbjct: 5   LPNPDPITVPLVLKLCSFPPPRRLF--SLRLRRFTRKSSSLLPLV-AVSSLSATAAKPTR 64

Query: 262 RREQIEL-ESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGD 321
            RE+ EL ESDSIS+LNERIRR  GKRE++R AMD+EEA++YI MVKEQQ+RGLQKLKG 
Sbjct: 65  WREKPELAESDSISLLNERIRRDLGKRETARPAMDSEEAEKYIHMVKEQQERGLQKLKGI 124

Query: 322 RQRKESDG---FNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYA 381
           RQ  ++ G   F+YKVDPY+L SGDYVVHKKVGIGRFVGIKFDV K S+EP+EYVF+EYA
Sbjct: 125 RQGTKAAGDGAFSYKVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEYVFIEYA 184

Query: 382 DGMAKLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMEL 441
           DGMAKLP+KQASR+LYRYNLPNETKRPR LS+L+DTS WE+RKTKGKVAIQKMVVDLMEL
Sbjct: 185 DGMAKLPLKQASRLLYRYNLPNETKRPRTLSRLSDTSVWERRKTKGKVAIQKMVVDLMEL 244

Query: 442 YLHRLKQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVG 501
           YLHRL+Q+R PYPK P M DFAAQFPY  T DQK+AF DV+KDLTERETPMDRLICGDVG
Sbjct: 245 YLHRLRQKRYPYPKNPIMADFAAQFPYNATPDQKQAFLDVEKDLTERETPMDRLICGDVG 304

Query: 502 FGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKA 561
           FGKTEVALRAIFCVVS GKQAMVLAPTIVLAKQH++VI++RFS +P +++GLLSRFQTKA
Sbjct: 305 FGKTEVALRAIFCVVSTGKQAMVLAPTIVLAKQHYDVISERFSLYPHIKVGLLSRFQTKA 364

Query: 562 EKEKHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSID 621
           EKE++L+MIK G L+IIVGTHSLLG RVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS+D
Sbjct: 365 EKEEYLEMIKTGHLNIIVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSVD 424

Query: 622 VLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGG 681
           VLTLSATPIPRTLYLALTGFRDASLI+TPPPER+PIKTHLS+F KEKV  AIK EL+RGG
Sbjct: 425 VLTLSATPIPRTLYLALTGFRDASLISTPPPERIPIKTHLSSFRKEKVIEAIKNELDRGG 484

Query: 682 QVFYVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNI 741
           QVFYVLPRIKGLEEV DFLE +FPDI+IA+AHGKQYSKQLEETME FA G IKILICTNI
Sbjct: 485 QVFYVLPRIKGLEEVMDFLEEAFPDIDIAMAHGKQYSKQLEETMERFAQGKIKILICTNI 544

Query: 742 VESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL 801
           VESGLDIQNANTII+QDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL
Sbjct: 545 VESGLDIQNANTIIIQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL 604

Query: 802 AALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHW 861
           +ALEECRELGQGFQLAE+DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLSKV+E  
Sbjct: 605 SALEECRELGQGFQLAEKDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLSKVEELR 664

Query: 862 VVSVPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHG 921
           + SVPY  VKI I+INP LPSEY+NYLENPM+II+ AEKAAE D+W+LMQFTENLRRQ+G
Sbjct: 665 IFSVPYDLVKIDININPRLPSEYVNYLENPMEIIHEAEKAAEKDMWSLMQFTENLRRQYG 724

Query: 922 KEPYSMEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNC 981
           KEPYSMEI+LKKLYVRRMAADLG+++IY SGK V M+T M+KKVFKLITDSMT +V+R+ 
Sbjct: 725 KEPYSMEIILKKLYVRRMAADLGVNRIYASGKMVVMKTNMSKKVFKLITDSMTCDVYRSS 784

Query: 982 LSFEEHQI 986
           L +E  QI
Sbjct: 785 LIYEGDQI 789

BLAST of Sgr025889 vs. ExPASy Swiss-Prot
Match: Q55750 (Transcription-repair-coupling factor OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=mfd PE=3 SV=1)

HSP 1 Score: 533.9 bits (1374), Expect = 4.7e-150
Identity = 298/654 (45.57%), Postives = 421/654 (64.37%), Query Frame = 0

Query: 323  RKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAK 382
            RK     + +VD   L  GDYVVHK  GIG+F  +K D         EY+ ++YADG+ +
Sbjct: 512  RKRRRATSKQVDINKLSPGDYVVHKSHGIGKF--LKLDALANR----EYLMIQYADGILR 571

Query: 383  LPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRL 442
            +P       L R+   +   RP  L K+     WE  K K + A++K+ VDL+ LY  R 
Sbjct: 572  VPADSLD-SLSRFR--HTGTRPPELHKMGG-KVWEATKNKVRKAVKKLAVDLLNLYAKRA 631

Query: 443  KQRRPPY-PKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKT 502
            KQ    Y P  P  ++    FPY+PT DQ KA  DV++DL E + PMDRL+CGDVGFGKT
Sbjct: 632  KQVGYAYPPDSPWQQELEDSFPYQPTPDQLKAVQDVKRDL-EGDRPMDRLVCGDVGFGKT 691

Query: 503  EVALRAIFCVVSAG-KQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKE 562
            EVA+RAIF  V++G KQ  +LAPT VL +QH+  + +RF+ +P + IGLL+RF+T +EK+
Sbjct: 692  EVAVRAIFKAVTSGNKQVALLAPTTVLTQQHYHTLKERFAPYP-ITIGLLNRFRTASEKK 751

Query: 563  KHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLT 622
            + L  +K G+LDI+VGT  +LG  V + +LGLLV+DEEQRFGV QKEKI + KT +DVLT
Sbjct: 752  EILAKLKSGELDIVVGTQQVLGTSVKFKDLGLLVIDEEQRFGVNQKEKIKTLKTEVDVLT 811

Query: 623  LSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVF 682
            L+ATPIPRTLY++L+G R+ SLITTPPP R PIKTHLS ++ E + +AI+ EL+RGGQVF
Sbjct: 812  LTATPIPRTLYMSLSGIREMSLITTPPPSRRPIKTHLSPYNPEVIRTAIRNELDRGGQVF 871

Query: 683  YVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVES 742
            YV+PRI+G+EE+   L    P   IA+ HG+    +LE TM  F  G+  IL+CT I+E+
Sbjct: 872  YVVPRIEGIEELGGQLRQMVPSARIAIGHGQMEESELESTMLAFNDGEADILVCTTIIEA 931

Query: 743  GLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAAL 802
            GLDI   NTIIV+D Q+FGLAQLYQLRGRVGR+  +AHA+L YP++  L+++A  RL AL
Sbjct: 932  GLDIPRVNTIIVEDAQKFGLAQLYQLRGRVGRSGIQAHAWLLYPNQKQLTEKARLRLRAL 991

Query: 803  EECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVS 862
            +E  +LG G+QLA RDM IRG G + G +Q+G +  +G + + EML D++ ++    +  
Sbjct: 992  QEFSQLGSGYQLATRDMEIRGVGNLLGAEQSGQMEAIGYEFYMEMLQDAIKEIQGQEIPK 1051

Query: 863  VPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEP 922
            V  +  +I + +   +PS+YI  LE  M           TD   L +   +   ++G  P
Sbjct: 1052 V--EDTQIDLPLTAFIPSDYIPDLEEKMAAYRRITSIESTD--ELPKIALDWGDRYGMLP 1111

Query: 923  YSMEILLKKLYVRRMAADLGISQIYTSGK-TVCMETEMNKKVFKLITDSMTSEV 974
              +E L K + ++ +A  LG S+I   GK  + +ET M +  +KL+ +++ + +
Sbjct: 1112 SPVEELFKVVKLKHLAKSLGFSRIKVEGKQNLVLETPMEEPAWKLLAENLPNHL 1149

BLAST of Sgr025889 vs. ExPASy Swiss-Prot
Match: Q49V12 (Transcription-repair-coupling factor OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=mfd PE=3 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 6.4e-123
Identity = 257/656 (39.18%), Postives = 406/656 (61.89%), Query Frame = 0

Query: 302  YIQMVKEQQQRGLQKLKGDRQRKESDGFN--YKVDPY-TLRSGDYVVHKKVGIGRFVGIK 361
            Y+Q+V    +R L K K  ++ K+        K+  Y  L+ GDYVVH   G+GR++G++
Sbjct: 459  YMQLV-VVTERELFKSKQKKKPKQHKTLTNAEKIKSYQDLKVGDYVVHVHHGVGRYLGVE 518

Query: 362  FDVQKGSTEPIEYVFMEY-ADGMAKLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWE 421
              ++ G     +Y+ ++Y       +PV Q  ++  +  + +E K P+ L+KL  T  W+
Sbjct: 519  -TLEVGGVHK-DYIKLQYKGTDQLFVPVDQMDQV--QKYVASEDKSPK-LNKLGGTE-WK 578

Query: 422  KRKTKGKVAIQKMVVDLMELYLHRLKQRRPPY-PKCPAMKDFAAQFPYEPTVDQKKAFSD 481
            K K K + +++ M  +L+ELY  R       + P      DF   FPYE T DQ K+  +
Sbjct: 579  KTKAKVQQSVEDMADELIELYKAREMSVGYKFGPDTAEQNDFEIDFPYELTPDQSKSIEE 638

Query: 482  VQKDLTERETPMDRLICGDVGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVIT 541
            +++D+ E E PMDRL+CGDVG+GKTEVA+RA F  V  GKQ   L PT +LA+QH+E + 
Sbjct: 639  IKQDM-EIERPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHYETLI 698

Query: 542  QRFSSFPDVQIGLLSRFQTKAEKEKHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVD 601
            +R   FP +++ L+SRF+T  E ++  + +K G +DI+VGTH LLG  + Y +LGLL+VD
Sbjct: 699  ERMQDFP-IEVQLISRFRTTKEVKETKEGLKSGFVDIVVGTHKLLGKDIQYKDLGLLIVD 758

Query: 602  EEQRFGVKQKEKIASFKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTH 661
            EEQRFGV+ KE+I S K ++DVLTL+ATPIPRTL++++ G RD S+I TPP  R P++T+
Sbjct: 759  EEQRFGVRHKERIKSLKNNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTY 818

Query: 662  LSTFSKEKVNSAIKYELERGGQVFYVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQ 721
            +   +   +  A++ EL R GQVFY+  +++ + E ++ L+   PD  I +AHG+   + 
Sbjct: 819  VLEQNTNFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANIGVAHGQMNERD 878

Query: 722  LEETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKE 781
            LEETM +F   +  I++ T I+E+G+D+ NANT+I++D  +FGL+QLYQLRGRVGR+ + 
Sbjct: 879  LEETMLSFINHEYDIIVTTTIIETGVDVPNANTLIIEDADRFGLSQLYQLRGRVGRSSRI 938

Query: 782  AHAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGN 841
             +AY  +P   +LS+ A +RL A++E  ELG GF++A RD+ IRG G + G+QQ G + +
Sbjct: 939  GYAYFLHPTNKVLSETAEDRLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDS 998

Query: 842  VGIDLFFEMLFDSLSKVDEHWVVSVPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEK 901
            VG DL+ +ML +++++             ++I ++I+ +LP+EYI   ++ ++I     K
Sbjct: 999  VGFDLYSQMLEEAVNEKRGIKAEKQDAPEIEIELNIDAYLPAEYIPNEQSKIEIYKKLRK 1058

Query: 902  -AAETDIWTLMQFTENLRRQHGKEPYSMEILLKKLYVRRMAADLGISQIYTSGKTV 952
              +ET    LM   + L  +    P  +E LL+ + ++  A   G++ I   GK V
Sbjct: 1059 IESETQ---LMDVKDELIDRFNDYPIEVERLLEMMEIKVHALHAGVTLIKDVGKQV 1102

BLAST of Sgr025889 vs. ExPASy Swiss-Prot
Match: Q4L3G0 (Transcription-repair-coupling factor OS=Staphylococcus haemolyticus (strain JCSC1435) OX=279808 GN=mfd PE=3 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 1.9e-122
Identity = 262/676 (38.76%), Postives = 413/676 (61.09%), Query Frame = 0

Query: 302  YIQMVKEQQQRGLQKLKGDRQRKESDGFN--YKVDPY-TLRSGDYVVHKKVGIGRFVGIK 361
            Y+Q+V    +R L K K  +QRK +   +   K+  Y  L  GDYVVH   G+GR++G++
Sbjct: 458  YMQLV-VITERELFKTKQKKQRKRTKTLSNAEKIKSYQDLNVGDYVVHVHHGVGRYLGVE 517

Query: 362  FDVQKGSTEPIEYVFMEY-ADGMAKLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWE 421
              ++ G     +Y+ ++Y       +PV Q  ++  +  + +E K P+ L+KL   S W+
Sbjct: 518  -TLEVGDVHR-DYIKLQYKGTDQLFVPVDQMDQV--QKYVASEDKSPK-LNKLGG-SEWK 577

Query: 422  KRKTKGKVAIQKMVVDLMELYLHRLKQRRPPYPKCPAMKD-FAAQFPYEPTVDQKKAFSD 481
            K K K + +++ +  +L+ LY  R       Y +  A +  F   FPYE T DQ K+  +
Sbjct: 578  KTKAKVQQSVEDIADELIALYKEREMSVGYQYGEDTAEQSAFEMDFPYELTPDQAKSIDE 637

Query: 482  VQKDLTERETPMDRLICGDVGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVIT 541
            ++ D+ ERE PMDRL+CGDVG+GKTEVA+RA F  V  GKQ   L PT +LA+QH+E + 
Sbjct: 638  IKGDM-ERERPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQHYETLI 697

Query: 542  QRFSSFPDVQIGLLSRFQTKAEKEKHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVD 601
            +R   FP VQI L+SRF++  E ++  + +K G +DI+VGTH LLG  + Y +LGLL+VD
Sbjct: 698  ERMQDFP-VQIELISRFRSTKEVKETKEGLKSGYVDIVVGTHKLLGKDIHYKDLGLLIVD 757

Query: 602  EEQRFGVKQKEKIASFKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTH 661
            EEQRFGV+ KE+I + KT++DVLTL+ATPIPRTL++++ G RD S+I TPP  R P++T+
Sbjct: 758  EEQRFGVRHKERIKTMKTNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTY 817

Query: 662  LSTFSKEKVNSAIKYELERGGQVFYVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQ 721
            +   +   +  A++ EL R GQVFY+  R++ + E ++ L+   PD  IA+AHG+   + 
Sbjct: 818  VLEQNSNFIKEALERELSRDGQVFYLYNRVQSIYEKREQLQMLMPDANIAVAHGQMTERD 877

Query: 722  LEETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKE 781
            LEETM +F  G+  IL+ T I+E+G+D+ NANT+I+++  +FGL+QLYQLRGRVGR+ + 
Sbjct: 878  LEETMLSFINGEFDILVTTTIIETGVDVPNANTLIIEEADRFGLSQLYQLRGRVGRSSRI 937

Query: 782  AHAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGN 841
             +AY  +    +L++ A ERL A++E  ELG GF++A RD+ IRG G + G+QQ G + +
Sbjct: 938  GYAYFLHSANKVLTETAEERLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDS 997

Query: 842  VGIDLFFEMLFDSLSKVDEHWVVSVPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEK 901
            VG DL+ +ML +++++             V++ ++++ +LP+EYI   +  ++I     K
Sbjct: 998  VGFDLYSQMLEEAVNEKRGIKEEEPDAPEVEMELNLDAYLPAEYIQNEQAKIEIYKKLRK 1057

Query: 902  AAETDIWTLMQFTENLRRQHGKEPYSMEILLKKLYVRRMAADLGISQIYTSGKTVCMETE 961
                +   L    + L  +    P  +E LL+ + ++  A   G++ I   GK +  E  
Sbjct: 1058 VETEE--QLFDIKDELIDRFNDYPVEVERLLEMVEIKIHALHAGVTLIKDKGKQI--EVS 1116

Query: 962  MNKKVFKLITDSMTSE 973
            ++ K     T+ M  E
Sbjct: 1118 LSTKA----TEQMNGE 1116

BLAST of Sgr025889 vs. ExPASy Swiss-Prot
Match: Q5HRQ2 (Transcription-repair-coupling factor OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) OX=176279 GN=mfd PE=3 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.7e-121
Identity = 254/655 (38.78%), Postives = 403/655 (61.53%), Query Frame = 0

Query: 302  YIQMVKEQQQRGLQKLKGDRQRKESDGFN--YKVDPY-TLRSGDYVVHKKVGIGRFVGIK 361
            Y+Q+V    +R L K +  +QRK +   +   K+  Y  L  GDY+VH   G+GR++G++
Sbjct: 458  YMQLV-VITERELFKTRQKKQRKRTKTISNAEKIKSYQDLNVGDYIVHVHHGVGRYLGVE 517

Query: 362  FDVQKGSTEPIEYVFMEY-ADGMAKLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWE 421
              ++ G T   +Y+ ++Y       +PV Q  ++  +  + +E K PR L+KL  T  W+
Sbjct: 518  -TLEVGDTHR-DYIKLQYKGTDQLFVPVDQMDQV--QKYVASEDKSPR-LNKLGGTE-WK 577

Query: 422  KRKTKGKVAIQKMVVDLMELYLHRLKQRRPPYPKCPAMKD-FAAQFPYEPTVDQKKAFSD 481
            K K K + +++ +  +L++LY  R       Y +  A +  F   FPYE T DQ K+  +
Sbjct: 578  KTKAKVQQSVEDIADELIDLYKEREMSVGYQYGQDTAEQSAFEHDFPYELTPDQSKSIDE 637

Query: 482  VQKDLTERETPMDRLICGDVGFGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVIT 541
            ++ D+ ER  PMDRL+CGDVG+GKTEVA+RA F  V  GKQ   L PT +LA+QH+E + 
Sbjct: 638  IKGDM-ERARPMDRLLCGDVGYGKTEVAVRAAFKAVMDGKQVAFLVPTTILAQQHYETLL 697

Query: 542  QRFSSFPDVQIGLLSRFQTKAEKEKHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVD 601
            +R   FP V+I L+SRF+T  E  +  + +K G +DI+VGTH LLG  + Y +LGLL+VD
Sbjct: 698  ERMQDFP-VEIQLVSRFRTAKEIRETKEGLKSGYVDIVVGTHKLLGKDIQYKDLGLLIVD 757

Query: 602  EEQRFGVKQKEKIASFKTSIDVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTH 661
            EEQRFGV+ KE+I + K ++DVLTL+ATPIPRTL++++ G RD S+I TPP  R P++T+
Sbjct: 758  EEQRFGVRHKERIKTLKKNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPPENRFPVQTY 817

Query: 662  LSTFSKEKVNSAIKYELERGGQVFYVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQ 721
            +   +   +  A++ EL R GQVFY+  +++ + E ++ L+   PD  IA+AHG+   + 
Sbjct: 818  VLEQNTNFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQRLMPDANIAVAHGQMTERD 877

Query: 722  LEETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKE 781
            LEETM +F   +  IL+ T I+E+G+D+ NANT+I+++  +FGL+QLYQLRGRVGR+ + 
Sbjct: 878  LEETMLSFINHEYDILVTTTIIETGVDVPNANTLIIEEADRFGLSQLYQLRGRVGRSSRI 937

Query: 782  AHAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGN 841
             +AY  +P   +L++ A ERL A++E  ELG GF++A RD+ IRG G + G+QQ G + +
Sbjct: 938  GYAYFLHPANKVLNETAEERLQAIKEFTELGSGFKIAMRDLNIRGAGNLLGKQQHGFIDS 997

Query: 842  VGIDLFFEMLFDSLSKVDEHWVVSVPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEK 901
            VG DL+ +ML +++++       S     +++ + ++ +LP+EYI   +  ++I     K
Sbjct: 998  VGFDLYSQMLEEAVNEKRGIKEESPDAPDIEVELHLDAYLPAEYIQSEQAKIEIYKKLRK 1057

Query: 902  AAETDIWTLMQFTENLRRQHGKEPYSMEILLKKLYVRRMAADLGISQIYTSGKTV 952
                +   L    + L  +    P  +E LL  + ++  A   G+  I   GK++
Sbjct: 1058 VETEE--QLFDVKDELIDRFNDYPIEVERLLDIVEIKVHALHAGVELIKDKGKSI 1101

BLAST of Sgr025889 vs. ExPASy TrEMBL
Match: A0A6J1CJT5 (ATP-dependent DNA helicase At3g02060, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011831 PE=4 SV=1)

HSP 1 Score: 1478.8 bits (3827), Expect = 0.0e+00
Identity = 747/783 (95.40%), Postives = 762/783 (97.32%), Query Frame = 0

Query: 204 NVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARRR 263
           +VSTFRP VAFRLGSFPR W LFNRNC+ R CPK CISVTNVVYAEDVMVPG  KSARRR
Sbjct: 9   DVSTFRPLVAFRLGSFPRQWRLFNRNCTLRRCPKRCISVTNVVYAEDVMVPGTAKSARRR 68

Query: 264 EQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR 323
           EQIELE DSIS+LNER+RRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR
Sbjct: 69  EQIELERDSISILNERVRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQR 128

Query: 324 KESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAKL 383
            ESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVF+EYADGMAKL
Sbjct: 129 NESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFIEYADGMAKL 188

Query: 384 PVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK 443
           PVKQASRMLYRYNLPNETKRPR LSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK
Sbjct: 189 PVKQASRMLYRYNLPNETKRPRTLSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRLK 248

Query: 444 QRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTEV 503
           QRRPPYPKC AM+DFAAQFPYEPTVDQK+AFSDV+KDLTERETPMDRLICGDVGFGKTEV
Sbjct: 249 QRRPPYPKCSAMEDFAAQFPYEPTVDQKEAFSDVEKDLTERETPMDRLICGDVGFGKTEV 308

Query: 504 ALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL 563
           ALRAIFCVVS+GKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL
Sbjct: 309 ALRAIFCVVSSGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKHL 368

Query: 564 DMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA 623
           DMIK GQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA
Sbjct: 369 DMIKEGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLSA 428

Query: 624 TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYVL 683
           TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKV SAIKYELERGGQVFYVL
Sbjct: 429 TPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQVFYVL 488

Query: 684 PRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD 743
           PRIKGLEEVKDFLE SFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD
Sbjct: 489 PRIKGLEEVKDFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGLD 548

Query: 744 IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC 803
           IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC
Sbjct: 549 IQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEEC 608

Query: 804 RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVPY 863
           RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEH VVSVPY
Sbjct: 609 RELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVPY 668

Query: 864 QSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYSM 923
           QSVKI +DI PHLPSEYINYLENPMKIINGAEKAAETDIW LMQFTENLRRQHGKEPYSM
Sbjct: 669 QSVKIDVDIKPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQHGKEPYSM 728

Query: 924 EILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEEH 983
           EILLKKLYVRRMAADLGIS+IY SGKTVCMET+MN+KVFKLITDSMTSEVH+NCLSFEEH
Sbjct: 729 EILLKKLYVRRMAADLGISRIYASGKTVCMETKMNRKVFKLITDSMTSEVHQNCLSFEEH 788

Query: 984 QIK 987
            IK
Sbjct: 789 HIK 791

BLAST of Sgr025889 vs. ExPASy TrEMBL
Match: A0A5A7VIC8 (ATP-dependent DNA helicase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold790G00090 PE=4 SV=1)

HSP 1 Score: 1426.8 bits (3692), Expect = 0.0e+00
Identity = 719/785 (91.59%), Postives = 754/785 (96.05%), Query Frame = 0

Query: 204 NVSTFRPPVAFRLGSFPRL--WGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSAR 263
           +VS+ RP +AF+L SFP L  WGLFNRNC+FRH P  C+SVTNV YAEDV+VPG  KSAR
Sbjct: 10  DVSSSRPLLAFKLRSFPTLWGWGLFNRNCTFRHFPNRCVSVTNVAYAEDVIVPGTAKSAR 69

Query: 264 RREQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDR 323
           RR+QIELE DSISVLNERI R+HGKR+SSRT MD+EEADRYIQMVKEQQQRGLQKLKGD+
Sbjct: 70  RRDQIELERDSISVLNERILRFHGKRDSSRTTMDSEEADRYIQMVKEQQQRGLQKLKGDK 129

Query: 324 QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMA 383
           QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFV IKFDVQKGSTEP EYVF+EYADGMA
Sbjct: 130 QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVRIKFDVQKGSTEPTEYVFIEYADGMA 189

Query: 384 KLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHR 443
           KLPVKQASRMLYRYNLPNE K+PR LSKLNDT+TWEKRKTKGKVAIQKMVVDLMELYLHR
Sbjct: 190 KLPVKQASRMLYRYNLPNENKKPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLMELYLHR 249

Query: 444 LKQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKT 503
           LKQRR PYPKC AM++F+AQFPYEPTVDQK+AF DV++DLTERETPMDRLICGDVGFGKT
Sbjct: 250 LKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTERETPMDRLICGDVGFGKT 309

Query: 504 EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK 563
           EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK
Sbjct: 310 EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK 369

Query: 564 HLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTL 623
           HL+MIK GQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS+DVLTL
Sbjct: 370 HLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSVDVLTL 429

Query: 624 SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFY 683
           SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKV SAIKYELERGGQ+FY
Sbjct: 430 SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQIFY 489

Query: 684 VLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG 743
           VLPRIKGLEEVK+FLE SFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG
Sbjct: 490 VLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG 549

Query: 744 LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALE 803
           LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD+ALERLAALE
Sbjct: 550 LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDEALERLAALE 609

Query: 804 ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSV 863
           ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEH VVSV
Sbjct: 610 ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSV 669

Query: 864 PYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPY 923
           PYQSVKI IDINPHLPSEYINYLENPMKI+NGAE+AAETDIWTLMQFTENLRR HGKEPY
Sbjct: 670 PYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRHHGKEPY 729

Query: 924 SMEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFE 983
           SMEILLKKLYVRRMAADLGIS+IY SGK VCMET MNKKVF+LI+DSMTSEVHRNCLSFE
Sbjct: 730 SMEILLKKLYVRRMAADLGISRIYASGKVVCMETNMNKKVFQLISDSMTSEVHRNCLSFE 789

Query: 984 EHQIK 987
           EHQIK
Sbjct: 790 EHQIK 794

BLAST of Sgr025889 vs. ExPASy TrEMBL
Match: A0A6J1GZL7 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111458673 PE=4 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 718/784 (91.58%), Postives = 754/784 (96.17%), Query Frame = 0

Query: 203 SNVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARR 262
           ++VS+ RP VAFRLGSFP+LWGLFNRNC+ RH  K C+S+TNVVYAEDVMVPGA KSARR
Sbjct: 8   ADVSSSRPLVAFRLGSFPKLWGLFNRNCNLRHFSKRCVSITNVVYAEDVMVPGAAKSARR 67

Query: 263 REQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQ 322
           REQIELE DSI+VLNERIRR+H KRESS+TAMDAEEADRYIQMVKEQQQRGLQKLKG+RQ
Sbjct: 68  REQIELEHDSITVLNERIRRFHVKRESSKTAMDAEEADRYIQMVKEQQQRGLQKLKGNRQ 127

Query: 323 RKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAK 382
           +KESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVF+EYADGMAK
Sbjct: 128 KKESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIEYADGMAK 187

Query: 383 LPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRL 442
           L VKQASRMLYRY+LPNE KRPR LSKLNDT+TWEKRKTKGK+AIQKMVVDLMELYLHRL
Sbjct: 188 LSVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLMELYLHRL 247

Query: 443 KQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTE 502
           KQRRPPYPKC AM++FAAQF Y PTVDQK+AF DV+KDLTERETPMDRLICGDVGFGKTE
Sbjct: 248 KQRRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGDVGFGKTE 307

Query: 503 VALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKH 562
           VALRAIF VVSAGKQAMVLAPTIVLAKQHFEVIT RFSSFPDVQIGLLSRFQTK+EK+KH
Sbjct: 308 VALRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITHRFSSFPDVQIGLLSRFQTKSEKDKH 367

Query: 563 LDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS 622
           L+MIK GQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS
Sbjct: 368 LEMIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS 427

Query: 623 ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYV 682
           ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKVNSAIKYELERGGQVFYV
Sbjct: 428 ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVNSAIKYELERGGQVFYV 487

Query: 683 LPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGL 742
            PRIKGLEEVKDFLE SFPDIEI LAHGKQYSKQLEETMENFALG IKILICTNIVESGL
Sbjct: 488 FPRIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFALGAIKILICTNIVESGL 547

Query: 743 DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEE 802
           DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALERLAALEE
Sbjct: 548 DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALERLAALEE 607

Query: 803 CRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVP 862
           CRELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDEH VVSVP
Sbjct: 608 CRELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVP 667

Query: 863 YQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYS 922
           YQSVKI IDINPHLPSEYINYLENPMKIINGAEKAAETDIW LMQFTENLRRQHGKEPYS
Sbjct: 668 YQSVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRQHGKEPYS 727

Query: 923 MEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEE 982
           MEILLKKLYVRRMAADLGI +IY SGKTVCMET+MNKKVFKLI++SM SEVHRNCL+FEE
Sbjct: 728 MEILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHRNCLTFEE 787

Query: 983 HQIK 987
           HQIK
Sbjct: 788 HQIK 791

BLAST of Sgr025889 vs. ExPASy TrEMBL
Match: A0A1S3CNQ6 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503000 PE=4 SV=1)

HSP 1 Score: 1424.5 bits (3686), Expect = 0.0e+00
Identity = 717/785 (91.34%), Postives = 754/785 (96.05%), Query Frame = 0

Query: 204 NVSTFRPPVAFRLGSFPRL--WGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSAR 263
           +VS+ RP +AF+L SFP L  WGLFNRNC+FRH P  C+SVTNV YAEDV+VPG  KSAR
Sbjct: 10  DVSSSRPLLAFKLRSFPTLWGWGLFNRNCTFRHFPNRCVSVTNVAYAEDVIVPGTAKSAR 69

Query: 264 RREQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDR 323
           RR+QIELE DSISVLNERI R+HGKR+SSRT MD+EEADRYIQMVKEQQQRGLQKLKGD+
Sbjct: 70  RRDQIELERDSISVLNERILRFHGKRDSSRTTMDSEEADRYIQMVKEQQQRGLQKLKGDK 129

Query: 324 QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMA 383
           QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFV IKFDVQKGSTEP EYVF+EYADGMA
Sbjct: 130 QRKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVRIKFDVQKGSTEPTEYVFIEYADGMA 189

Query: 384 KLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHR 443
           KLPVKQASRMLYRYNLPNE ++PR LSKLNDT+TWEKRKTKGKVAIQKMVVDLMELYLHR
Sbjct: 190 KLPVKQASRMLYRYNLPNENRKPRTLSKLNDTTTWEKRKTKGKVAIQKMVVDLMELYLHR 249

Query: 444 LKQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKT 503
           LKQRR PYPKC AM++F+AQFPYEPTVDQK+AF DV++DLTERETPMDRLICGDVGFGKT
Sbjct: 250 LKQRRSPYPKCSAMEEFSAQFPYEPTVDQKEAFRDVERDLTERETPMDRLICGDVGFGKT 309

Query: 504 EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK 563
           EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK
Sbjct: 310 EVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEK 369

Query: 564 HLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTL 623
           HL+MIK GQL+IIVGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS+DVLTL
Sbjct: 370 HLEMIKEGQLNIIVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSVDVLTL 429

Query: 624 SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFY 683
           SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS+FSKEKV SAIKYELERGGQ+FY
Sbjct: 430 SATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSSFSKEKVKSAIKYELERGGQIFY 489

Query: 684 VLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG 743
           VLPRIKGLEEVK+FLE SFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG
Sbjct: 490 VLPRIKGLEEVKEFLEASFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESG 549

Query: 744 LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALE 803
           LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEA+AYLFYPDKSLLSD+ALERLAALE
Sbjct: 550 LDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAYAYLFYPDKSLLSDEALERLAALE 609

Query: 804 ECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSV 863
           +CRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEH VVSV
Sbjct: 610 DCRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHRVVSV 669

Query: 864 PYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPY 923
           PYQSVKI IDINPHLPSEYINYLENPMKI+NGAE+AAETDIWTLMQFTENLRR HGKEPY
Sbjct: 670 PYQSVKIDIDINPHLPSEYINYLENPMKILNGAERAAETDIWTLMQFTENLRRHHGKEPY 729

Query: 924 SMEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFE 983
           SMEILLKKLYVRRMAADLGIS+IY SGK VCMET MNKKVF+LI+DSMTSEVHRNCLSFE
Sbjct: 730 SMEILLKKLYVRRMAADLGISRIYASGKVVCMETNMNKKVFQLISDSMTSEVHRNCLSFE 789

Query: 984 EHQIK 987
           EHQIK
Sbjct: 790 EHQIK 794

BLAST of Sgr025889 vs. ExPASy TrEMBL
Match: A0A6J1JT22 (ATP-dependent DNA helicase At3g02060, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111487597 PE=4 SV=1)

HSP 1 Score: 1411.7 bits (3653), Expect = 0.0e+00
Identity = 712/784 (90.82%), Postives = 749/784 (95.54%), Query Frame = 0

Query: 203 SNVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSARR 262
           ++VS+ RP VAFRLGSFP+LWGLFNRNC+ RHC K  +S+TNVVYAEDVMV G  KSARR
Sbjct: 8   ADVSSSRPLVAFRLGSFPKLWGLFNRNCNLRHCSKRFVSITNVVYAEDVMVSGTAKSARR 67

Query: 263 REQIELESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGDRQ 322
           REQIELE DSI+VLNERIRR+H KRESS+TAMDA EADRYIQMVKEQQQRGLQKLKG+R+
Sbjct: 68  REQIELERDSITVLNERIRRFHVKRESSKTAMDAVEADRYIQMVKEQQQRGLQKLKGNRK 127

Query: 323 RKESDGFNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYADGMAK 382
           +KESDG++YKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEP EYVF+EYADGMAK
Sbjct: 128 KKESDGYDYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPTEYVFIEYADGMAK 187

Query: 383 LPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMELYLHRL 442
           L VKQASRMLYRY+LPNE KRPR LSKLNDT+TWEKRKTKGK+AIQKMVVDLMELYLHRL
Sbjct: 188 LSVKQASRMLYRYSLPNENKRPRTLSKLNDTTTWEKRKTKGKIAIQKMVVDLMELYLHRL 247

Query: 443 KQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTE 502
           KQRRPPYPKC AM++FAAQF Y PTVDQK+AF DV+KDLTERETPMDRLICGDVGFGKTE
Sbjct: 248 KQRRPPYPKCSAMEEFAAQFSYTPTVDQKEAFRDVEKDLTERETPMDRLICGDVGFGKTE 307

Query: 503 VALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKAEKEKH 562
           VALRAIF VVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTK+EK+KH
Sbjct: 308 VALRAIFYVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKSEKDKH 367

Query: 563 LDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS 622
           L+MIK GQL+I+VGTHSLLGDRVVY+NLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS
Sbjct: 368 LEMIKEGQLNIVVGTHSLLGDRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSIDVLTLS 427

Query: 623 ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGGQVFYV 682
           ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLS FSKEKV SAIKYELERGGQVFYV
Sbjct: 428 ATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSAFSKEKVKSAIKYELERGGQVFYV 487

Query: 683 LPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNIVESGL 742
            PRIKGLEEVKDFLE SFPDIEI LAHGKQYSKQLEETMENF+LG IKILICTNIVESGL
Sbjct: 488 FPRIKGLEEVKDFLEASFPDIEIGLAHGKQYSKQLEETMENFSLGAIKILICTNIVESGL 547

Query: 743 DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERLAALEE 802
           DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDK+LLSDQALERLAALEE
Sbjct: 548 DIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKALLSDQALERLAALEE 607

Query: 803 CRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHWVVSVP 862
           CRELGQGFQLAERDMGIRGFGTIFGEQQ+GDVGNVGIDLFFEMLFDSLSKVDEH VVSVP
Sbjct: 608 CRELGQGFQLAERDMGIRGFGTIFGEQQSGDVGNVGIDLFFEMLFDSLSKVDEHRVVSVP 667

Query: 863 YQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHGKEPYS 922
           YQSVKI IDINPHLPSEYINYLENPMKIINGAEKAAETDIW LMQFTENLRR HGKEPYS
Sbjct: 668 YQSVKIDIDINPHLPSEYINYLENPMKIINGAEKAAETDIWALMQFTENLRRHHGKEPYS 727

Query: 923 MEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNCLSFEE 982
           MEILLKKLYVRRMAADLGI +IY SGKTVCMET+MNKKVFKLI++SM SEVHRNCL+FEE
Sbjct: 728 MEILLKKLYVRRMAADLGIYRIYASGKTVCMETKMNKKVFKLISESMVSEVHRNCLTFEE 787

Query: 983 HQIK 987
           HQIK
Sbjct: 788 HQIK 791

BLAST of Sgr025889 vs. TAIR 10
Match: AT3G02060.1 (DEAD/DEAH box helicase, putative )

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 616/788 (78.17%), Postives = 691/788 (87.69%), Query Frame = 0

Query: 202 LSNVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSAR 261
           L N      P+  +L SFP    LF  +   R   +   S+  +V A   +   A K  R
Sbjct: 5   LPNPDPITVPLVLKLCSFPPPRRLF--SLRLRRFTRKSSSLLPLV-AVSSLSATAAKPTR 64

Query: 262 RREQIEL-ESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGD 321
            RE+ EL ESDSIS+LNERIRR  GKRE++R AMD+EEA++YI MVKEQQ+RGLQKLKG 
Sbjct: 65  WREKPELAESDSISLLNERIRRDLGKRETARPAMDSEEAEKYIHMVKEQQERGLQKLKGI 124

Query: 322 RQRKESDG---FNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYA 381
           RQ  ++ G   F+YKVDPY+L SGDYVVHKKVGIGRFVGIKFDV K S+EP+EYVF+EYA
Sbjct: 125 RQGTKAAGDGAFSYKVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEYVFIEYA 184

Query: 382 DGMAKLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMEL 441
           DGMAKLP+KQASR+LYRYNLPNETKRPR LS+L+DTS WE+RKTKGKVAIQKMVVDLMEL
Sbjct: 185 DGMAKLPLKQASRLLYRYNLPNETKRPRTLSRLSDTSVWERRKTKGKVAIQKMVVDLMEL 244

Query: 442 YLHRLKQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVG 501
           YLHRL+Q+R PYPK P M DFAAQFPY  T DQK+AF DV+KDLTERETPMDRLICGDVG
Sbjct: 245 YLHRLRQKRYPYPKNPIMADFAAQFPYNATPDQKQAFLDVEKDLTERETPMDRLICGDVG 304

Query: 502 FGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKA 561
           FGKTEVALRAIFCVVS GKQAMVLAPTIVLAKQH++VI++RFS +P +++GLLSRFQTKA
Sbjct: 305 FGKTEVALRAIFCVVSTGKQAMVLAPTIVLAKQHYDVISERFSLYPHIKVGLLSRFQTKA 364

Query: 562 EKEKHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSID 621
           EKE++L+MIK G L+IIVGTHSLLG RVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS+D
Sbjct: 365 EKEEYLEMIKTGHLNIIVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSVD 424

Query: 622 VLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGG 681
           VLTLSATPIPRTLYLALTGFRDASLI+TPPPER+PIKTHLS+F KEKV  AIK EL+RGG
Sbjct: 425 VLTLSATPIPRTLYLALTGFRDASLISTPPPERIPIKTHLSSFRKEKVIEAIKNELDRGG 484

Query: 682 QVFYVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNI 741
           QVFYVLPRIKGLEEV DFLE +FPDI+IA+AHGKQYSKQLEETME FA G IKILICTNI
Sbjct: 485 QVFYVLPRIKGLEEVMDFLEEAFPDIDIAMAHGKQYSKQLEETMERFAQGKIKILICTNI 544

Query: 742 VESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL 801
           VESGLDIQNANTII+QDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL
Sbjct: 545 VESGLDIQNANTIIIQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL 604

Query: 802 AALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHW 861
           +ALEECRELGQGFQLAE+DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLSKV+E  
Sbjct: 605 SALEECRELGQGFQLAEKDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLSKVEELR 664

Query: 862 VVSVPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHG 921
           + SVPY  VKI I+INP LPSEY+NYLENPM+II+ AEKAAE D+W+LMQFTENLRRQ+G
Sbjct: 665 IFSVPYDLVKIDININPRLPSEYVNYLENPMEIIHEAEKAAEKDMWSLMQFTENLRRQYG 724

Query: 922 KEPYSMEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNC 981
           KEPYSMEI+LKKLYVRRMAADLG+++IY SGK V M+T M+KKVFKLITDSMT +V+R+ 
Sbjct: 725 KEPYSMEIILKKLYVRRMAADLGVNRIYASGKMVVMKTNMSKKVFKLITDSMTCDVYRSS 784

Query: 982 LSFEEHQI 986
           L +E  QI
Sbjct: 785 LIYEGDQI 789

BLAST of Sgr025889 vs. TAIR 10
Match: AT3G02060.2 (DEAD/DEAH box helicase, putative )

HSP 1 Score: 1185.2 bits (3065), Expect = 0.0e+00
Identity = 616/788 (78.17%), Postives = 691/788 (87.69%), Query Frame = 0

Query: 202 LSNVSTFRPPVAFRLGSFPRLWGLFNRNCSFRHCPKPCISVTNVVYAEDVMVPGAVKSAR 261
           L N      P+  +L SFP    LF  +   R   +   S+  +V A   +   A K  R
Sbjct: 4   LPNPDPITVPLVLKLCSFPPPRRLF--SLRLRRFTRKSSSLLPLV-AVSSLSATAAKPTR 63

Query: 262 RREQIEL-ESDSISVLNERIRRYHGKRESSRTAMDAEEADRYIQMVKEQQQRGLQKLKGD 321
            RE+ EL ESDSIS+LNERIRR  GKRE++R AMD+EEA++YI MVKEQQ+RGLQKLKG 
Sbjct: 64  WREKPELAESDSISLLNERIRRDLGKRETARPAMDSEEAEKYIHMVKEQQERGLQKLKGI 123

Query: 322 RQRKESDG---FNYKVDPYTLRSGDYVVHKKVGIGRFVGIKFDVQKGSTEPIEYVFMEYA 381
           RQ  ++ G   F+YKVDPY+L SGDYVVHKKVGIGRFVGIKFDV K S+EP+EYVF+EYA
Sbjct: 124 RQGTKAAGDGAFSYKVDPYSLLSGDYVVHKKVGIGRFVGIKFDVPKDSSEPLEYVFIEYA 183

Query: 382 DGMAKLPVKQASRMLYRYNLPNETKRPRALSKLNDTSTWEKRKTKGKVAIQKMVVDLMEL 441
           DGMAKLP+KQASR+LYRYNLPNETKRPR LS+L+DTS WE+RKTKGKVAIQKMVVDLMEL
Sbjct: 184 DGMAKLPLKQASRLLYRYNLPNETKRPRTLSRLSDTSVWERRKTKGKVAIQKMVVDLMEL 243

Query: 442 YLHRLKQRRPPYPKCPAMKDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVG 501
           YLHRL+Q+R PYPK P M DFAAQFPY  T DQK+AF DV+KDLTERETPMDRLICGDVG
Sbjct: 244 YLHRLRQKRYPYPKNPIMADFAAQFPYNATPDQKQAFLDVEKDLTERETPMDRLICGDVG 303

Query: 502 FGKTEVALRAIFCVVSAGKQAMVLAPTIVLAKQHFEVITQRFSSFPDVQIGLLSRFQTKA 561
           FGKTEVALRAIFCVVS GKQAMVLAPTIVLAKQH++VI++RFS +P +++GLLSRFQTKA
Sbjct: 304 FGKTEVALRAIFCVVSTGKQAMVLAPTIVLAKQHYDVISERFSLYPHIKVGLLSRFQTKA 363

Query: 562 EKEKHLDMIKRGQLDIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIASFKTSID 621
           EKE++L+MIK G L+IIVGTHSLLG RVVY+NLGLLVVDEEQRFGVKQKEKIASFKTS+D
Sbjct: 364 EKEEYLEMIKTGHLNIIVGTHSLLGSRVVYSNLGLLVVDEEQRFGVKQKEKIASFKTSVD 423

Query: 622 VLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSKEKVNSAIKYELERGG 681
           VLTLSATPIPRTLYLALTGFRDASLI+TPPPER+PIKTHLS+F KEKV  AIK EL+RGG
Sbjct: 424 VLTLSATPIPRTLYLALTGFRDASLISTPPPERIPIKTHLSSFRKEKVIEAIKNELDRGG 483

Query: 682 QVFYVLPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQLEETMENFALGDIKILICTNI 741
           QVFYVLPRIKGLEEV DFLE +FPDI+IA+AHGKQYSKQLEETME FA G IKILICTNI
Sbjct: 484 QVFYVLPRIKGLEEVMDFLEEAFPDIDIAMAHGKQYSKQLEETMERFAQGKIKILICTNI 543

Query: 742 VESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL 801
           VESGLDIQNANTII+QDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL
Sbjct: 544 VESGLDIQNANTIIIQDVQQFGLAQLYQLRGRVGRADKEAHAYLFYPDKSLLSDQALERL 603

Query: 802 AALEECRELGQGFQLAERDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFDSLSKVDEHW 861
           +ALEECRELGQGFQLAE+DMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLF+SLSKV+E  
Sbjct: 604 SALEECRELGQGFQLAEKDMGIRGFGTIFGEQQTGDVGNVGIDLFFEMLFESLSKVEELR 663

Query: 862 VVSVPYQSVKIGIDINPHLPSEYINYLENPMKIINGAEKAAETDIWTLMQFTENLRRQHG 921
           + SVPY  VKI I+INP LPSEY+NYLENPM+II+ AEKAAE D+W+LMQFTENLRRQ+G
Sbjct: 664 IFSVPYDLVKIDININPRLPSEYVNYLENPMEIIHEAEKAAEKDMWSLMQFTENLRRQYG 723

Query: 922 KEPYSMEILLKKLYVRRMAADLGISQIYTSGKTVCMETEMNKKVFKLITDSMTSEVHRNC 981
           KEPYSMEI+LKKLYVRRMAADLG+++IY SGK V M+T M+KKVFKLITDSMT +V+R+ 
Sbjct: 724 KEPYSMEIILKKLYVRRMAADLGVNRIYASGKMVVMKTNMSKKVFKLITDSMTCDVYRSS 783

Query: 982 LSFEEHQI 986
           L +E  QI
Sbjct: 784 LIYEGDQI 788

BLAST of Sgr025889 vs. TAIR 10
Match: AT2G01440.1 (DEAD/DEAH box RNA helicase family protein )

HSP 1 Score: 214.5 bits (545), Expect = 4.5e-55
Identity = 142/416 (34.13%), Postives = 219/416 (52.64%), Query Frame = 0

Query: 456 KDFAAQFPYEPTVDQKKAFSDVQKDLTERETPMDRLICGDVGFGKTEVALRAIFCVVSAG 515
           K F    PY  T  Q  A S++  DL +R  PM+RL+ GDVG GKT VA  A   V+ +G
Sbjct: 512 KSFLKALPYSLTPSQLSAVSEIIWDL-KRPVPMNRLLQGDVGCGKTVVAFLACMEVIGSG 571

Query: 516 KQAMVLAPTIVLAKQHFEVITQRFSSFPDVQ----IGLLSRFQTKAEKEKHLDMIKRGQL 575
            QA  +APT +LA QH+E       +   V     IGLL+      +       ++ G +
Sbjct: 572 YQAAFMAPTELLAIQHYEQCRDLLENMEGVSSKPTIGLLTGSTPAKQSRMIRQDLQSGAI 631

Query: 576 DIIVGTHSLLGDRVVYNNLGLLVVDEEQRFGVKQKEKIAS--FKTSI------------- 635
             I+GTHSL+ +++ Y+ L + VVDE+QRFGV Q+ K  S  + TS+             
Sbjct: 632 SFIIGTHSLIAEKIEYSALRIAVVDEQQRFGVIQRGKFNSKLYGTSMISKSGSSDSDDTS 691

Query: 636 --------DVLTLSATPIPRTLYLALTGFRDASLITTPPPERVPIKTHLSTFSK---EKV 695
                    VL +SATPIPR+L LAL G    + IT  P  R+P++TH+   ++   ++V
Sbjct: 692 KADLSMAPHVLAMSATPIPRSLALALYGDISLTQITGMPLGRIPVETHIFEGNETGIKEV 751

Query: 696 NSAIKYELERGGQVFYV---------LPRIKGLEEVKDFLETSFPDIEIALAHGKQYSKQ 755
            S +  +L+ GG+V+ V         LP+++      + +   FP     L HG+  S  
Sbjct: 752 YSMMLEDLKSGGRVYVVYPVIDQSEQLPQLRAASAELEIVTKKFPKYNCGLLHGRMKSDD 811

Query: 756 LEETMENFALGDIKILICTNIVESGLDIQNANTIIVQDVQQFGLAQLYQLRGRVGRADKE 815
            EE +  F  G+ +IL+ T ++E G+D+ +A+ ++V + ++FG+AQL+QLRGRVGR  ++
Sbjct: 812 KEEALNKFRSGETQILLSTQVIEIGVDVPDASMMVVMNAERFGIAQLHQLRGRVGRGTRK 871

Query: 816 AHAYLFYPDKSLLSDQALERLAALEECRELGQGFQLAERDMGIRGFGTIFGEQQTG 833
           +        K LL   +   L  L    +   GF LA  D+ +RG G + G++Q+G
Sbjct: 872 S--------KCLLVGSSTNSLKRLNMLGKSSDGFYLANIDLLLRGPGDLLGKKQSG 918

BLAST of Sgr025889 vs. TAIR 10
Match: AT5G13910.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 167.5 bits (423), Expect = 6.3e-41
Identity = 107/213 (50.23%), Postives = 127/213 (59.62%), Query Frame = 0

Query: 1   MSPSPSKPKRKQPQPPETTSHRFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAAL 60
           M+ + SK K+KQ     T   RFLGVRRRPWGRYAAEIRDP+TKERHWLGTFDTAEEAAL
Sbjct: 1   MNTTSSKSKKKQDDQVGT---RFLGVRRRPWGRYAAEIRDPTTKERHWLGTFDTAEEAAL 60

Query: 61  AYDRAARSMRGSLARTNFLYADSQPASAVTSIISPDQPLLPPPSLFLPLVPPDPGP---- 120
           AYDRAARSMRG+ ARTNF+Y+D  P+S+VTSI+SPD P  PPP    P  PP   P    
Sbjct: 61  AYDRAARSMRGTRARTNFVYSDMPPSSSVTSIVSPDDPPPPPP----PPAPPSNDPVDYM 120

Query: 121 -FFAAGAGDDSSELPPFPPAI-------SSEPENYCYGLMETQNVGFDQSSIGIG----- 180
             F   +  DS  L P    +       S    +YCY    +  +    S +        
Sbjct: 121 MMFNQYSSTDSPMLQPHCDQVDSYMFGGSQSSNSYCYSNDSSNELPPLPSDLSNSCYSQP 180

Query: 181 --TYFGFDSSSEYVHTPLFGTMPAVSDAVAGDF 195
             T+ G D SSEYVH+P+F  MP VSD+    F
Sbjct: 181 QWTWTGDDYSSEYVHSPMFSRMPPVSDSFPQGF 206

BLAST of Sgr025889 vs. TAIR 10
Match: AT1G28160.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 124.4 bits (311), Expect = 6.1e-28
Identity = 66/105 (62.86%), Postives = 81/105 (77.14%), Query Frame = 0

Query: 7   KPKRKQPQPPETTSH-RFLGVRRRPWGRYAAEIRDPSTKERHWLGTFDTAEEAALAYDRA 66
           K ++ QPQP +     +++GVRRRPWGRYAAEIR+P+TKER+WLGTFDTAEEAALAYDRA
Sbjct: 22  KQQQPQPQPQQHIEEIKYVGVRRRPWGRYAAEIRNPTTKERYWLGTFDTAEEAALAYDRA 81

Query: 67  ARSMRGSLARTNFLYADSQPASAVTSIISPDQ------PLLPPPS 105
           ARS+RG  ARTNF+Y+D    S+VTS +SPD+       L  PPS
Sbjct: 82  ARSIRGLTARTNFVYSDMPRGSSVTSFVSPDESQRFISELFNPPS 126