Sgr025836 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025836 vs. NCBI nr
Match: XP_022152158.1 (polyribonucleotide nucleotidyltransferase 2, mitochondrial [Momordica charantia])

HSP 1 Score: 1559.7 bits (4037), Expect = 0.0e+00
Identity = 827/943 (87.70%), Postives = 854/943 (90.56%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDPDTAVAG-TKVLETF 108
           MASMA+KANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS Q+LDPDT +A  TKVLETF
Sbjct: 1   MASMASKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQSQQQLDPDTTLASRTKVLETF 60

Query: 109 KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQEK 168
            EEFEIGSRLITLETGKIARFANGAAVLGME+TKVLSTVAS+KGD+VRDFLPLTVDYQEK
Sbjct: 61  TEEFEIGSRLITLETGKIARFANGAAVLGMEDTKVLSTVASSKGDSVRDFLPLTVDYQEK 120

Query: 169 QFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDP 228
           QFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDP
Sbjct: 121 QFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDP 180

Query: 229 DVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKT 288
           DVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNP M+ELNLSDLNLVYACTREKT
Sbjct: 181 DVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPTMNELNLSDLNLVYACTREKT 240

Query: 289 LMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKV 348
           LMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQ+RLA KAGKLKKEYKLSMVSDSTMEKV
Sbjct: 241 LMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQVRLAAKAGKLKKEYKLSMVSDSTMEKV 300

Query: 349 TKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKV 408
           TKLAEAPIEAVFTDPSYGKFERGEAL+ ITQDVKR FEEE DEE LKVLPKAVDYVRKKV
Sbjct: 301 TKLAEAPIEAVFTDPSYGKFERGEALENITQDVKRTFEEECDEEGLKVLPKAVDYVRKKV 360

Query: 409 VRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQR 468
           VRRRIISEGLRVDGRRLDEVRPLYCES YLP+LHGS+IF+RGDTQVLCTVTLGAPGDAQR
Sbjct: 361 VRRRIISEGLRVDGRRLDEVRPLYCESSYLPVLHGSSIFSRGDTQVLCTVTLGAPGDAQR 420

Query: 469 LESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYT 528
           LESLVGPPTKR                             TLAEKALLAVLPPENEFPYT
Sbjct: 421 LESLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPPENEFPYT 480

Query: 529 VRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRI 588
           VR+TSEVMASDGSTSMATVCGGSMALMDAGIPLN+HVAGVSVGLVSE DPSTGAIKDYRI
Sbjct: 481 VRITSEVMASDGSTSMATVCGGSMALMDAGIPLNQHVAGVSVGLVSETDPSTGAIKDYRI 540

Query: 589 LTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHM 648
           LTDILGLEDHLGDMDFKIAGTR+GITAIQLDIKPAGIPL+IICESLEPAR GRLQILEHM
Sbjct: 541 LTDILGLEDHLGDMDFKIAGTREGITAIQLDIKPAGIPLDIICESLEPARKGRLQILEHM 600

Query: 649 EREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTIL 708
           EREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTIL
Sbjct: 601 EREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTIL 660

Query: 709 AKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHD 768
           AKNQSVMEKVQEKVDFTLGREIEIGG+YKGVV SVKEYGAFVEFNGGQQGLLHISELSHD
Sbjct: 661 AKNQSVMEKVQEKVDFTLGREIEIGGVYKGVVASVKEYGAFVEFNGGQQGLLHISELSHD 720

Query: 769 SVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVGE 828
            VSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEG NGN        PPV E
Sbjct: 721 PVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGRNGN------LFPPVSE 780

Query: 829 AFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKDA 888
             K R ETLN  +NTSPVD  V SE N +SSIPSILIRSAEDCDEEEK+S GLNLKLK+ 
Sbjct: 781 VSKMRHETLN--ANTSPVDKGVGSEVNPTSSIPSILIRSAEDCDEEEKRSAGLNLKLKNT 840

Query: 889 QKPNANSRSERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLDLG 948
           +K NA S+S      FTQN VN NEVE   PITA+NLKLGMKVRAKVYQ+R  GLVLDLG
Sbjct: 841 RKLNAMSKS------FTQNDVNMNEVE-SLPITARNLKLGMKVRAKVYQVRVGGLVLDLG 900

Query: 949 EGLRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           EGLRGMYRFE DNRSNFEVGDELHVQCSSF+ KGIP  S V +
Sbjct: 901 EGLRGMYRFEGDNRSNFEVGDELHVQCSSFTVKGIPVMSLVEN 928

BLAST of Sgr025836 vs. NCBI nr
Match: XP_023531117.1 (polyribonucleotide nucleotidyltransferase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1543.9 bits (3996), Expect = 0.0e+00
Identity = 815/945 (86.24%), Postives = 858/945 (90.79%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDP-DTAVAGTKVLETF 108
           MASMA+KA+PLFS IPHFLTWRSLGFRT+CCGRMGFASQS Q+LDP DT VA TKVLE+F
Sbjct: 1   MASMASKAHPLFSAIPHFLTWRSLGFRTVCCGRMGFASQSQQQLDPTDTTVARTKVLESF 60

Query: 109 KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAV-RDFLPLTVDYQE 168
           KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTV SAKGDAV  DFLPLTVDYQE
Sbjct: 61  KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVTSAKGDAVSSDFLPLTVDYQE 120

Query: 169 KQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 228
           KQFAQGVIP+TFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD
Sbjct: 121 KQFAQGVIPSTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 180

Query: 229 PDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREK 288
           P VMAANATSAALMLSDIPWGGPIGVIRIGR++GQFVVNP MDELNLSDLNLVYACTREK
Sbjct: 181 PGVMAANATSAALMLSDIPWGGPIGVIRIGRVDGQFVVNPTMDELNLSDLNLVYACTREK 240

Query: 289 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEK 348
           TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSM+S+STMEK
Sbjct: 241 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMLSESTMEK 300

Query: 349 VTKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKK 408
           VTKLAEAPIEAVFTD SYGKFERGEALDKIT+DVK+ FEEE DEE LKVLPKAVD VRKK
Sbjct: 301 VTKLAEAPIEAVFTDHSYGKFERGEALDKITKDVKKVFEEECDEEGLKVLPKAVDSVRKK 360

Query: 409 VVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQ 468
           VVRRRII+EGLRVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPG+AQ
Sbjct: 361 VVRRRIIAEGLRVDGRRLDEVRPLYCESRYLPILHGSSIFSRGDTQVLCTVTLGAPGEAQ 420

Query: 469 RLESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPY 528
           RL+SLVGPPTKR                             TLAEKALLAVLP E+EFPY
Sbjct: 421 RLDSLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPSEDEFPY 480

Query: 529 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYR 588
           TVRVTSEVMASDGSTSMATVCGGSMALMDAGIP+N HVAGVSVGL+SE DP+TGAI DYR
Sbjct: 481 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPINAHVAGVSVGLISETDPTTGAIIDYR 540

Query: 589 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEH 648
           ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GRLQILEH
Sbjct: 541 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRLQILEH 600

Query: 649 MEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTI 708
           MEREINAPRIKDDKNSPRL  LKYTNDALRRLIGPLGA KKKIEEETGARISVGDGTLTI
Sbjct: 601 MEREINAPRIKDDKNSPRLVILKYTNDALRRLIGPLGASKKKIEEETGARISVGDGTLTI 660

Query: 709 LAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 768
           LAKNQ+VMEKVQEKVDFTLGREIEIGG+YKGVVTSVKEYGAFVEFNGGQQGLLHISELSH
Sbjct: 661 LAKNQAVMEKVQEKVDFTLGREIEIGGMYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 720

Query: 769 DSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVG 828
           D VSRVSDVVSVGQ+ISL+CIGQDVRGNIKLSLKAT+P PKAE  NGNSVPS +SV  VG
Sbjct: 721 DPVSRVSDVVSVGQRISLVCIGQDVRGNIKLSLKATMPRPKAEAQNGNSVPSLDSVSRVG 780

Query: 829 EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKD 888
           E  KT  ET NSTSNTSPVD  V SE N +SS+PSILIRSAEDCDEEEKKS  +N+KLKD
Sbjct: 781 EVSKTLHETPNSTSNTSPVDISVGSEVNSTSSVPSILIRSAEDCDEEEKKSADMNVKLKD 840

Query: 889 AQKPNANSRSERKSKPFTQN-GVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLD 948
            +K NANSRSERK KP  QN G + NEV+V+SPITA+NLKLG++VRAKVYQIRARGLVLD
Sbjct: 841 TRKSNANSRSERKLKPLAQNGGPSNNEVDVKSPITARNLKLGVRVRAKVYQIRARGLVLD 900

Query: 949 LGEGLRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           LGEGLRGMY+FE DNRSNFEVG+ELHVQCSSFS+KGIP  S V +
Sbjct: 901 LGEGLRGMYKFEGDNRSNFEVGEELHVQCSSFSTKGIPVMSLVDN 945

BLAST of Sgr025836 vs. NCBI nr
Match: XP_022928433.1 (polyribonucleotide nucleotidyltransferase 2, mitochondrial [Cucurbita moschata] >XP_022928434.1 polyribonucleotide nucleotidyltransferase 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1543.5 bits (3995), Expect = 0.0e+00
Identity = 815/945 (86.24%), Postives = 857/945 (90.69%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDP-DTAVAGTKVLETF 108
           MASMA+KA+PLFS IPHFLTWRSLGFRT+CCGRMGFASQS Q+LDP DT VA +KVLE+F
Sbjct: 1   MASMASKAHPLFSAIPHFLTWRSLGFRTVCCGRMGFASQSQQQLDPSDTTVARSKVLESF 60

Query: 109 KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAV-RDFLPLTVDYQE 168
           KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTV SAKGDAV  DFLPLTVDYQE
Sbjct: 61  KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVTSAKGDAVSSDFLPLTVDYQE 120

Query: 169 KQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 228
           KQFAQGVIP+TFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD
Sbjct: 121 KQFAQGVIPSTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 180

Query: 229 PDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREK 288
           P VMAANATSAALMLSDIPWGGPIGVIRIGR++GQFVVNP MDELNLSDLNLVYACTREK
Sbjct: 181 PGVMAANATSAALMLSDIPWGGPIGVIRIGRVDGQFVVNPTMDELNLSDLNLVYACTREK 240

Query: 289 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEK 348
           TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSM+S+STMEK
Sbjct: 241 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMLSESTMEK 300

Query: 349 VTKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKK 408
           VTKLAEAPIEAVFTD SYGKFERGEALD IT+DVK+ FEEE DEE LKVLPKAVD VRKK
Sbjct: 301 VTKLAEAPIEAVFTDHSYGKFERGEALDNITKDVKKVFEEECDEEGLKVLPKAVDSVRKK 360

Query: 409 VVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQ 468
           VVRRRII+EGLRVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPG+AQ
Sbjct: 361 VVRRRIIAEGLRVDGRRLDEVRPLYCESRYLPILHGSSIFSRGDTQVLCTVTLGAPGEAQ 420

Query: 469 RLESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPY 528
           RL+SLVGPPTKR                             TLAEKALLAVLP E+EFPY
Sbjct: 421 RLDSLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPSEDEFPY 480

Query: 529 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYR 588
           TVRVTSEVMASDGSTSMATVCGGSMALMDAGIP+N HVAGVSVGLVSE DP+TGAI DYR
Sbjct: 481 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPINAHVAGVSVGLVSETDPTTGAIIDYR 540

Query: 589 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEH 648
           ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GRLQILEH
Sbjct: 541 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRLQILEH 600

Query: 649 MEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTI 708
           MEREINAPRIKDD+NSPRL  LKYTNDALRRLIGPLGA KKKIEEETGARISVGDGTLTI
Sbjct: 601 MEREINAPRIKDDENSPRLVILKYTNDALRRLIGPLGASKKKIEEETGARISVGDGTLTI 660

Query: 709 LAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 768
           LAKNQ+VMEKVQEKVDFTLGREIEIGG+YKGVVTSVKEYGAFVEFNGGQQGLLHISELSH
Sbjct: 661 LAKNQAVMEKVQEKVDFTLGREIEIGGMYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 720

Query: 769 DSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVG 828
           D VSRVSDVVSVGQ+ISL+CIGQDVRGNIKLSLKAT+P PKAE  NGNSVPS + V PVG
Sbjct: 721 DPVSRVSDVVSVGQRISLVCIGQDVRGNIKLSLKATMPRPKAEAQNGNSVPSLDPVSPVG 780

Query: 829 EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKD 888
           E  KT  ET NSTSNTSPVD  V SE N +SS+PSILIRSAEDCDEEEKKS  +N+KLKD
Sbjct: 781 EVSKTLHETPNSTSNTSPVDISVGSEVNSTSSVPSILIRSAEDCDEEEKKSADMNVKLKD 840

Query: 889 AQKPNANSRSERKSKPFTQN-GVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLD 948
            +K NANSRSERK KP  QN G + NEVEV+SPITA+NLKLG++VRAKVYQIRARGLVLD
Sbjct: 841 TRKSNANSRSERKLKPLAQNGGPSNNEVEVKSPITARNLKLGVRVRAKVYQIRARGLVLD 900

Query: 949 LGEGLRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           LGEGLRGMY+FE DNRSNFEVGDELHVQCSSFS+KGIP  S V +
Sbjct: 901 LGEGLRGMYKFEGDNRSNFEVGDELHVQCSSFSTKGIPVMSLVDN 945

BLAST of Sgr025836 vs. NCBI nr
Match: KAG6588949.1 (Polyribonucleotide nucleotidyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1538.5 bits (3982), Expect = 0.0e+00
Identity = 812/945 (85.93%), Postives = 856/945 (90.58%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDP-DTAVAGTKVLETF 108
           MASMA+KA+PLFS IPHFLTWRSLGFRT+CCGRMGFASQS Q+LDP DT VA +KVLE+F
Sbjct: 1   MASMASKAHPLFSAIPHFLTWRSLGFRTVCCGRMGFASQSQQQLDPSDTTVARSKVLESF 60

Query: 109 KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAV-RDFLPLTVDYQE 168
           KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTV +AKGDAV  DFLPLTVDYQE
Sbjct: 61  KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVTAAKGDAVSSDFLPLTVDYQE 120

Query: 169 KQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 228
           KQFAQGVIP+TFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD
Sbjct: 121 KQFAQGVIPSTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 180

Query: 229 PDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREK 288
           P VMAANATSAALMLSDIPWGGPIGVIRIGR++GQFVVNP MDELNLSDLNLVYACTREK
Sbjct: 181 PGVMAANATSAALMLSDIPWGGPIGVIRIGRVDGQFVVNPTMDELNLSDLNLVYACTREK 240

Query: 289 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEK 348
           TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSM+S+STMEK
Sbjct: 241 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMLSESTMEK 300

Query: 349 VTKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKK 408
           VTKLAEAPIEAVFTD SYGKFERGEALD IT+DVK+ FEEE DEE LKVLPKAVD VRKK
Sbjct: 301 VTKLAEAPIEAVFTDHSYGKFERGEALDNITKDVKKVFEEECDEEGLKVLPKAVDSVRKK 360

Query: 409 VVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQ 468
           VVRRRII+EGLRVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPG+AQ
Sbjct: 361 VVRRRIIAEGLRVDGRRLDEVRPLYCESRYLPILHGSSIFSRGDTQVLCTVTLGAPGEAQ 420

Query: 469 RLESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPY 528
           RL+SLVGPPTKR                             TLAEKALLAVLP E+EFPY
Sbjct: 421 RLDSLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPSEDEFPY 480

Query: 529 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYR 588
           TVRVTSEVMASDGSTSMATVCGGSMALMDAGIP+N HVAGVSVGLVSE DP+TGAI DYR
Sbjct: 481 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPINAHVAGVSVGLVSETDPTTGAIIDYR 540

Query: 589 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEH 648
           ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GRLQILEH
Sbjct: 541 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRLQILEH 600

Query: 649 MEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTI 708
           MEREINAPRIKDD+NSPRL  LKYTNDALRRLIGPLGA KKKIEEETGARISVGDGTLTI
Sbjct: 601 MEREINAPRIKDDENSPRLVILKYTNDALRRLIGPLGASKKKIEEETGARISVGDGTLTI 660

Query: 709 LAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 768
           LAKNQ+VMEKVQEKVDFTLGREIEIGG+YKGVVTSVKEYGAFVEFNGGQQGLLHISELSH
Sbjct: 661 LAKNQAVMEKVQEKVDFTLGREIEIGGMYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 720

Query: 769 DSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVG 828
           D VSRVSDVVSVGQ+ISL+CIGQDVRGNIKLSLKAT+P PKAE  NGNSVPS + V PVG
Sbjct: 721 DPVSRVSDVVSVGQRISLVCIGQDVRGNIKLSLKATMPRPKAEAQNGNSVPSLDPVSPVG 780

Query: 829 EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKD 888
           E  KT  ET NSTSNTSPV   V SE N +SS+PSILIRSAEDCDEEEKKS  +N+KLKD
Sbjct: 781 EVSKTLHETPNSTSNTSPVGISVGSEVNSTSSVPSILIRSAEDCDEEEKKSADMNVKLKD 840

Query: 889 AQKPNANSRSERKSKPFTQN-GVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLD 948
            +K NANSRSERK KP  QN G + NEVEV+SPITA+NLKLG++VRAKVYQIRARGLVLD
Sbjct: 841 TRKSNANSRSERKLKPLAQNGGPSNNEVEVKSPITARNLKLGVRVRAKVYQIRARGLVLD 900

Query: 949 LGEGLRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           LGEGLRGMY+FE DNRSNFEVGDELHVQCSSFS++GIP  S V +
Sbjct: 901 LGEGLRGMYKFEGDNRSNFEVGDELHVQCSSFSTRGIPVMSLVDN 945

BLAST of Sgr025836 vs. NCBI nr
Match: XP_022989223.1 (polyribonucleotide nucleotidyltransferase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1536.9 bits (3978), Expect = 0.0e+00
Identity = 809/941 (85.97%), Postives = 853/941 (90.65%), Query Frame = 0

Query: 52  MATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDPDTAVAG-TKVLETFKEE 111
           MA+KA+PLFS IPHFLTWRSL FRT+CCGRMGFASQS Q+ DP  A++G TKVLE+FKEE
Sbjct: 1   MASKAHPLFSAIPHFLTWRSLSFRTVCCGRMGFASQSQQQRDPTDAISGRTKVLESFKEE 60

Query: 112 FEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAV-RDFLPLTVDYQEKQF 171
           FEIGSRLITLETGKIARFANGAAVLGMEETKVLSTV SAKGDAV  DFLPLTVDYQEKQF
Sbjct: 61  FEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVTSAKGDAVCSDFLPLTVDYQEKQF 120

Query: 172 AQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPDV 231
           AQGVIP+TFMRREGAPKERELLCGRIIDRPIRPLFP+GFYHEVQVMASVLSSDGKQDP V
Sbjct: 121 AQGVIPSTFMRREGAPKERELLCGRIIDRPIRPLFPSGFYHEVQVMASVLSSDGKQDPGV 180

Query: 232 MAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTLM 291
           MAANATSAALMLSDIPWGGPIGVIRIGR++GQFVVNP MDELNLSDLNLVYACTREKTLM
Sbjct: 181 MAANATSAALMLSDIPWGGPIGVIRIGRVDGQFVVNPTMDELNLSDLNLVYACTREKTLM 240

Query: 292 IDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVTK 351
           IDVQA EITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSM+SDSTMEKVTK
Sbjct: 241 IDVQACEITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMLSDSTMEKVTK 300

Query: 352 LAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKVVR 411
           LAEAPIEAVFTD SYGKFERGEALDKIT+DV++ FEEE DEE LKVLPKAVD VRKKVVR
Sbjct: 301 LAEAPIEAVFTDHSYGKFERGEALDKITKDVEKVFEEECDEEGLKVLPKAVDRVRKKVVR 360

Query: 412 RRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRLE 471
           RRII+EGLRVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPG+AQRL+
Sbjct: 361 RRIIAEGLRVDGRRLDEVRPLYCESRYLPILHGSSIFSRGDTQVLCTVTLGAPGEAQRLD 420

Query: 472 SLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYTVR 531
           SLVGPPTKR                             TLAEKALLAVLP E+EFPYTVR
Sbjct: 421 SLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPSEDEFPYTVR 480

Query: 532 VTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILT 591
           VTSEVMASDGSTSMATVCGGSMALMDAGIP+N HVAGVSVGLVSE DP+TGAI DYRILT
Sbjct: 481 VTSEVMASDGSTSMATVCGGSMALMDAGIPINAHVAGVSVGLVSETDPTTGAIIDYRILT 540

Query: 592 DILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHMER 651
           DILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GRLQILEHME 
Sbjct: 541 DILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRLQILEHMES 600

Query: 652 EINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTILAK 711
           EINAPRIKDD NSPRL  LKYTNDALRRLIGPLGA KKKIEEETGARISVGDGTLTILAK
Sbjct: 601 EINAPRIKDDANSPRLVILKYTNDALRRLIGPLGASKKKIEEETGARISVGDGTLTILAK 660

Query: 712 NQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDSV 771
           NQ+VMEKVQEKVDFTLGREIEIGG+YKGVVTSVKEYGAFVEFNGGQQGLLHISELSHD V
Sbjct: 661 NQAVMEKVQEKVDFTLGREIEIGGMYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDPV 720

Query: 772 SRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVGEAF 831
           SRVSDVVSVGQ+ISL+CIGQDVRGNIKLSLKAT+P PKAE  NGNSVPS + V PVGE  
Sbjct: 721 SRVSDVVSVGQRISLVCIGQDVRGNIKLSLKATMPRPKAEAQNGNSVPSLDPVSPVGEVP 780

Query: 832 KTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKDAQK 891
           KT  ET NSTSNTSPVDT V SE NR+SSIPSILIRSAEDCDEE+KKS  +N+KLKD +K
Sbjct: 781 KTWHETPNSTSNTSPVDTSVGSEVNRTSSIPSILIRSAEDCDEEDKKSADMNVKLKDTRK 840

Query: 892 PNANSRSERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLDLGEG 951
            NANSRSERKSKP  QNG + NEV+V+SPI A+NLKLG++VRAKVYQIRARGLVLDLGEG
Sbjct: 841 SNANSRSERKSKPLAQNGPSNNEVDVESPINARNLKLGVRVRAKVYQIRARGLVLDLGEG 900

Query: 952 LRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           LRGMY+FE DNRSNFEVGDELHVQCSSFSSKGIP  S V++
Sbjct: 901 LRGMYKFEGDNRSNFEVGDELHVQCSSFSSKGIPVMSLVAN 941

BLAST of Sgr025836 vs. ExPASy Swiss-Prot
Match: Q9S7G6 (Polyribonucleotide nucleotidyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=PNP2 PE=2 SV=1)

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 616/999 (61.66%), Postives = 749/999 (74.97%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQSQRLDPDTAV-AGTKVLETFK 108
           M+S+  +A+   +++P+FL WR+LGFRTIC GR+GFA       PD+ V AGTK+LE+FK
Sbjct: 1   MSSIVNRASS--ASLPNFLAWRALGFRTICSGRLGFAPSV----PDSPVSAGTKILESFK 60

Query: 109 EEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQEKQ 168
           EEFE+GSR+++ ETGKIARFANG+ VLGM+ETKVLSTV  AK D+ RDFLPLTVDYQEKQ
Sbjct: 61  EEFEVGSRVVSFETGKIARFANGSVVLGMDETKVLSTVTCAKTDSPRDFLPLTVDYQEKQ 120

Query: 169 FAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPD 228
           +AQG+IPNT+MRREGAPKERELLCGR+IDRPIRPLFP GFYHEVQ+MASVLSSDGKQDPD
Sbjct: 121 YAQGLIPNTYMRREGAPKERELLCGRLIDRPIRPLFPTGFYHEVQIMASVLSSDGKQDPD 180

Query: 229 VMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTL 288
           ++AANA+SAALMLSD+PWGGPIGVIRIGRI GQFVVNP MDEL+ SDLNL+YACTR+KT+
Sbjct: 181 ILAANASSAALMLSDVPWGGPIGVIRIGRICGQFVVNPTMDELSSSDLNLIYACTRDKTM 240

Query: 289 MIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVT 348
           MIDVQ+REI+EKDL A LRLAHPEAVK+L+PQIRLA+KAGK KKEYKLSM+SD T+EKV 
Sbjct: 241 MIDVQSREISEKDLAAALRLAHPEAVKYLDPQIRLAEKAGKQKKEYKLSMLSDKTLEKVA 300

Query: 349 KLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKVV 408
            LA   IE+VFTDPSYGKFERGEALD I +DV++ FEEE D+ESL +LPKAVD VRKKVV
Sbjct: 301 DLAATRIESVFTDPSYGKFERGEALDNIGKDVRKVFEEEGDQESLSILPKAVDTVRKKVV 360

Query: 409 RRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRL 468
           R R+IS+G RVDGR +DEVRP+YCES YLP LHGSA+F+RGDTQVLCTVTLGAP +AQ L
Sbjct: 361 RSRMISDGFRVDGRHVDEVRPIYCESHYLPALHGSALFSRGDTQVLCTVTLGAPAEAQSL 420

Query: 469 ESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYTV 528
           +SLVGPP KR                             TLAEKALLAVLPPE  FPYT+
Sbjct: 421 DSLVGPPKKRFMLHYSFPPYCTNEVGKRGGLNRREVGHGTLAEKALLAVLPPEEAFPYTI 480

Query: 529 RVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRIL 588
           R+ SEVM+SDGSTSMA+VCGGSMALMDAGIPL  HVAGVSVGL++++DPS+G IKDYRI+
Sbjct: 481 RINSEVMSSDGSTSMASVCGGSMALMDAGIPLRAHVAGVSVGLITDVDPSSGEIKDYRIV 540

Query: 589 TDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHME 648
           TDILGLEDHLGDMDFKIAGTR G+TAIQLDIKPAGIPL+I+CESLE AR  RLQIL+HME
Sbjct: 541 TDILGLEDHLGDMDFKIAGTRDGVTAIQLDIKPAGIPLDIVCESLENAREARLQILDHME 600

Query: 649 REINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTILA 708
           R IN+PR +D   SPRLATLKY+ND+LR LIGP+G LK+KIE ETGAR+S+ +GTLTI+A
Sbjct: 601 RNINSPRGQDGAYSPRLATLKYSNDSLRTLIGPMGVLKRKIEVETGARLSIDNGTLTIVA 660

Query: 709 KNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDS 768
           KNQ VMEK QE+VDF +GRE+ +GG+YKG V+S+KEYGAFVEF GGQQGLLH+SELSH+ 
Sbjct: 661 KNQDVMEKAQEQVDFIIGRELVVGGVYKGTVSSIKEYGAFVEFPGGQQGLLHMSELSHEP 720

Query: 769 VSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSES--VPPVG 828
           VS+VSDV+ +GQ I+ MCI  DVRGNIKLS KA LP PK +       P+S++   P + 
Sbjct: 721 VSKVSDVLDIGQCITTMCIETDVRGNIKLSRKALLPKPKRK-------PASDAGKDPVMK 780

Query: 829 EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEK----------- 888
           E+     E  +     + + +IV        S+P+++IR+A +C+E EK           
Sbjct: 781 ESSTVYIENSSVGEIVASMPSIVTPLQKSRLSVPAVVIRTAVECNEAEKSSPVNDNDKPR 840

Query: 889 -----------KSTGLNL------------------------------KLKDAQKPNANS 948
                      KST   L                              KLK     N ++
Sbjct: 841 RAATSKPDRKPKSTASKLIATQKEEEALESIAPEETSAECGEILKQDGKLKSVSPKNNST 900

Query: 949 RS-----ERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLDLGEG 959
            S      +  K   +  ++ N+ E  + ++ + LK+G ++ A V  +RA GLVLDLG  
Sbjct: 901 ASNLVSFSKAKKSTMKENLSENKAEESASVSTRKLKIGTEMTATVDHVRALGLVLDLGGE 960

BLAST of Sgr025836 vs. ExPASy Swiss-Prot
Match: Q6KAI0 (Polyribonucleotide nucleotidyltransferase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=PNP2 PE=2 SV=1)

HSP 1 Score: 1084.7 bits (2804), Expect = 0.0e+00
Identity = 592/944 (62.71%), Postives = 707/944 (74.89%), Query Frame = 0

Query: 83  GFASQSQRLD-PDTAVAGTKVLETFKEEFEIGSRLITLETGKIARFANGAAVLGMEETKV 142
           G A +  + D P     G KVLE+F+EEFEIG R+I+ ETGK+ARFANG+ V+ M++T V
Sbjct: 37  GAAEEVAQADAPPPPPPGRKVLESFREEFEIGGRVISFETGKMARFANGSVVISMDDTHV 96

Query: 143 LSTVASAK-GDAVRDFLPLTVDYQEKQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIR 202
           LSTVA+AK  + VRDFLPLTVDYQEKQ+AQGVIP T+MRREGAPKERELLCGRIIDRPIR
Sbjct: 97  LSTVAAAKSSEPVRDFLPLTVDYQEKQYAQGVIPTTYMRREGAPKERELLCGRIIDRPIR 156

Query: 203 PLFPAGFYHEVQV------MASVLSSDGKQDPDVMAANATSAALMLSDIPWGGPIGVIRI 262
           PLFP GFYHEVQV      M +V+SSDGKQDPDVMAANA+SAALMLSDIPW GPIGVIR+
Sbjct: 157 PLFPPGFYHEVQVMNATIIMVNVISSDGKQDPDVMAANASSAALMLSDIPWNGPIGVIRV 216

Query: 263 GRINGQFVVNPAMDELNLSDLNLVYACTREKTLMIDVQAREITEKDLEAGLRLAHPEAVK 322
           GRI+G FV+NP +DEL LSDLNLVYAC+R+KTLMIDVQAREITE+DL+AG++LAH EAVK
Sbjct: 217 GRIDGNFVLNPTVDELGLSDLNLVYACSRDKTLMIDVQAREITERDLQAGMKLAHAEAVK 276

Query: 323 FLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVTKLAEAPIEAVFTDPSYGKFERGEALDK 382
            + PQ+RLA +AGK KKEYK+S++SD + EK+  L+EAPIE VFTD +YGKFERGEAL+ 
Sbjct: 277 CINPQLRLAKRAGKKKKEYKISLISDKSYEKIRTLSEAPIEEVFTDSTYGKFERGEALEN 336

Query: 383 ITQDVKRAFEEESDEESLKVLPKAVDYVRKKVVRRRIISEGLRVDGRRLDEVRPLYCESC 442
           ITQ VK   EEE DE+SLK L KAVD VRK+V+R+RII +GLRVDGR+LDEVRPLYCES 
Sbjct: 337 ITQSVKAKLEEECDEDSLKFLHKAVDTVRKQVIRKRIIEKGLRVDGRQLDEVRPLYCESS 396

Query: 443 YLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRLESLVGPPTKR----------------- 502
             PILHGSA+F+RGDTQVLCTVTLGAPGDAQRL+S+VGPPTKR                 
Sbjct: 397 TYPILHGSALFSRGDTQVLCTVTLGAPGDAQRLDSIVGPPTKRFMLHYSFPPFSINEVAK 456

Query: 503 ------------TLAEKALLAVLPPENEFPYTVRVTSEVMASDGSTSMATVCGGSMALMD 562
                       TLAEKALLAVLPPE EFPYTVRV SEVMASDGSTSMA+VCGGSMALMD
Sbjct: 457 RGGLNRREVGHGTLAEKALLAVLPPEGEFPYTVRVNSEVMASDGSTSMASVCGGSMALMD 516

Query: 563 AGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILTDILGLEDHLGDMDFKIAGTRKGITAI 622
           AGIP+ EHVAGVSVGLVSE+D +TG I  YRILTDILGLEDHLGDMDFKIAGTR+GITAI
Sbjct: 517 AGIPVREHVAGVSVGLVSEVDQTTGDISSYRILTDILGLEDHLGDMDFKIAGTRRGITAI 576

Query: 623 QLDIKPAGIPLEIICESLEPARNGRLQILEHMEREINAPRIKDDKNSPRLATLKYTNDAL 682
           QLDIKPAGIPL+IICESLEPAR  R QIL+ M++EI++ R  +D +SPRLATL +++D+L
Sbjct: 577 QLDIKPAGIPLDIICESLEPARKARNQILDRMDQEISSARAFNDGSSPRLATLSFSSDSL 636

Query: 683 RRLIGPLGALKKKIEEETGARISVGDGTLTILAKNQSVMEKVQEKVDFTLGREIEIGGIY 742
           R+L+      +KKIE+ETGAR+SV DGT+TI+AK Q +M+K  EKV+F +GREIE+G  Y
Sbjct: 637 RKLL----FHRKKIEQETGARVSVSDGTVTIVAKTQPIMDKAIEKVEFLVGREIEVGRTY 696

Query: 743 KGVVTSVKEYGAFVEFNGGQQGLLHISELSHDSVSRVSDVVSVGQKISLMCIGQDVRGNI 802
           KGVV+S+KEYGAFVEFNGGQQGLLHISELSHD VS+VSDVVSVGQ +SL CIGQD+RGNI
Sbjct: 697 KGVVSSIKEYGAFVEFNGGQQGLLHISELSHDKVSKVSDVVSVGQVLSLTCIGQDLRGNI 756

Query: 803 KLSLKATLP-GPKAEGWNGNSVPSSESVPPVG-EAFKTRPETLNSTSNTSPVDTIVASEA 862
           KLSLKATLP   + +    N      S   VG  A +  P    +   +   D     E 
Sbjct: 757 KLSLKATLPHAHEKKDLASNHTDPLPSQEVVGWTAVENMPSKDANAEPSISKDEDNMIEE 816

Query: 863 NRSSSIPSILIRSAEDCDEEE----------------------------------KKSTG 922
               S P+++IRSA +CD ++                                  KK++G
Sbjct: 817 TPGCSTPAVIIRSAAECDAQDVTNDPKKKRPKVAKSSPKLSKPASERQEVKRTSAKKTSG 876

Query: 923 LNLKLKDAQKPNANSRSERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRA 954
            +   K  +K  A+S ++       QN  N       S   + ++KLG  V AKVYQIRA
Sbjct: 877 ASTTAKKNKKEKADSSNDVLDAIPEQNKSNIMNYSSPSNFRSGSMKLGDVVTAKVYQIRA 936

BLAST of Sgr025836 vs. ExPASy Swiss-Prot
Match: B3DZ86 (Polyribonucleotide nucleotidyltransferase OS=Methylacidiphilum infernorum (isolate V4) OX=481448 GN=pnp PE=3 SV=2)

HSP 1 Score: 527.7 bits (1358), Expect = 2.8e-148
Identity = 295/705 (41.84%), Postives = 439/705 (62.27%), Query Frame = 0

Query: 104 ETFKEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASA---KGDAVRDFLPLT 163
           E  K +F  G + I  ETGK+A+FA+G+  +   ET VL T  S    K D  +DFLPL 
Sbjct: 3   EYIKRKF--GDQTIVFETGKLAKFADGSVTVSYGETAVLVTAVSVTELKED--QDFLPLQ 62

Query: 164 VDYQEKQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSS 223
           V+Y+EK  A G  P  + R+EG P ++E+L  R+IDRP+RPLFP G+++E Q++ ++LS+
Sbjct: 63  VEYREKAAAAGRFPGGYFRKEGRPTDKEILTSRMIDRPLRPLFPKGYFYETQILGTLLSA 122

Query: 224 DGKQDPDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYA 283
           DG+ DPDVMA N  SAALM+SDIP+ GP+G +RIG+I+G++++NP   E  +SD++LVY 
Sbjct: 123 DGQNDPDVMAINGASAALMVSDIPFYGPVGAVRIGQIDGRWIINPTHREREISDIDLVYV 182

Query: 284 CTREKTLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSD 343
            +    LMI+  ARE  E++    L  AH    + +  Q  LA+K GK K+   L   + 
Sbjct: 183 GSEHYPLMIEGSAREFPEEEFVKALEFAHQHVQEVIAAQKELAEKVGKPKRSCVLFEENL 242

Query: 344 STMEKVTKLAEAPI-EAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAV 403
           +   ++ +L  + + +A++T     K ER + L K+ +++     EE    + + +  + 
Sbjct: 243 AITNRLAELYSSQLQDALYTS---SKTERQKKLAKLKEELTAKILEEFPTATPREITSSY 302

Query: 404 DYVRKKVVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLG 463
           D ++K + R  ++    R DGR  +++RP+  E+  +P  HGS++F RG+TQ LC  TL 
Sbjct: 303 DSLQKTIFRTNVLETKRRCDGRGPEDIRPIAIETSVVPRSHGSSLFCRGETQALCMATLA 362

Query: 464 APGDAQRLESLVG-------------PP------------TKR-----TLAEKALLAVLP 523
           +  +AQ L++  G             PP            ++R      LAE++LL V+P
Sbjct: 363 SLNEAQELDAYGGGEQSKRFLLHYFFPPFSVGEVGRITGQSRREIGHGALAERSLLPVIP 422

Query: 524 PENEFPYTVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPST 583
            E +FPY +RV+SE++ S+GSTSMATVCGGS+ALMDAG+PL   VAG+SVGLV   +   
Sbjct: 423 SETDFPYAIRVSSEILESNGSTSMATVCGGSLALMDAGVPLKTSVAGISVGLVKGKEEDF 482

Query: 584 GAIKDYRILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNG 643
              + Y +LTDILGLEDH GDMDFKIAGTRKGIT  QLD+K  GIPL+++ +++  ++  
Sbjct: 483 DWSR-YCLLTDILGLEDHYGDMDFKIAGTRKGITGFQLDLKLRGIPLDVMAKAIFQSQKA 542

Query: 644 RLQILEHMEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISV 703
           R+ IL+ M++ INAPR +  K++PR+  +K   D +  LIGP G   KKI  E+GA I++
Sbjct: 543 RMVILDCMDKVINAPRAEISKHAPRIEKIKIHPDKIGLLIGPGGKTIKKISAESGAEITI 602

Query: 704 -GDGTLTILAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGL 763
             DGT+ I + +   +E  +E ++  +G E+ +GGIY+  V SVK++G F+E  G  +GL
Sbjct: 603 EDDGTVMIYSSSADSLEAAREMIEDMVG-EVTVGGIYRSKVVSVKDFGCFIEIKGKGEGL 662

Query: 764 LHISELSHDSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATL 774
           +HISELS   V RV  VV VG++I + CIG D +G  K S KA +
Sbjct: 663 VHISELSDTPVRRVDQVVRVGEEIWVKCIGVDEKGRYKFSRKAAM 698

BLAST of Sgr025836 vs. ExPASy Swiss-Prot
Match: C5D9D5 (Polyribonucleotide nucleotidyltransferase OS=Geobacillus sp. (strain WCH70) OX=471223 GN=pnp PE=3 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 1.1e-141
Identity = 287/708 (40.54%), Postives = 432/708 (61.02%), Query Frame = 0

Query: 103 LETFKEEFEI--GSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLT 162
           +E  K  F I    R + +E G++A+ ANGA ++   +T VL+T  ++K     DF PLT
Sbjct: 1   MEQEKRIFSIDWAGRPLVVEIGQLAKQANGAVLVRYGDTVVLNTATASKEAKNVDFFPLT 60

Query: 163 VDYQEKQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSS 222
           V+Y+E+ +A G IP  F++REG P E+ +L  R+IDRPIRPLF  GF +EVQ+++ V+S 
Sbjct: 61  VNYEERLYAVGKIPGGFIKREGRPSEKAILASRLIDRPIRPLFAEGFRNEVQIVSMVMSV 120

Query: 223 DGKQDPDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYA 282
           D    P++ A    S AL +SDIP+ GPI  + +GR++G+FV+NP +++   SD++LV A
Sbjct: 121 DQDCSPEMAALFGASLALTISDIPFEGPIAGVTVGRVDGEFVINPTVEQSEKSDIHLVVA 180

Query: 283 CTREKTLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSD 342
            T++   M++  A E+ E+ +   +   H E  + +  Q  +A + GK K E  L  +  
Sbjct: 181 GTKDAINMVEAGADEVPEEVILEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYELDP 240

Query: 343 STMEKVTKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEE-ESDEESLKVLPKAV 402
               ++ +LAE  I+     P   K  R  A++++   V   +EE E+DEE+LK + + +
Sbjct: 241 QLEAEIRQLAEEDIKRAVQVPE--KLARDAAIEEVKASVIAKYEEQEADEETLKQVNEIL 300

Query: 403 DYVRKKVVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLG 462
             + K+ VRR I  E +R DGR++DE+RPL  E   LP  HGS +F RG TQVL   TLG
Sbjct: 301 HKLVKEEVRRLITEEKIRPDGRKIDEIRPLSSEVGVLPRTHGSGLFTRGQTQVLSVCTLG 360

Query: 463 APGDAQRLESLVGPPTKR-----------------------------TLAEKALLAVLPP 522
           A GD Q L+ L    TKR                              L E+AL  V+P 
Sbjct: 361 ALGDVQILDGLGIEETKRFMHHYNFPPFSVGETGPMRGPGRREIGHGALGERALEPVVPS 420

Query: 523 ENEFPYTVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTG 582
           E EFPYT+R+ SEV+ S+GSTS A++C  ++A+MDAG+P+   VAG+++GLV   D    
Sbjct: 421 EKEFPYTIRLVSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNDD---- 480

Query: 583 AIKDYRILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGR 642
              +Y ILTDI G+EDHLGDMDFK+AGT KG+TA+Q+DIK  G+  EI+ E+L+ A+ GR
Sbjct: 481 ---NYTILTDIQGIEDHLGDMDFKVAGTAKGVTALQMDIKIKGLSREILEEALQQAKKGR 540

Query: 643 LQILEHMEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISV- 702
           ++ILEHM + I  PR +  K +P++ T++   + +R +IGP G    KI +ETG +I + 
Sbjct: 541 MEILEHMMQTIREPRKELSKYAPKILTMQINPEKIREVIGPSGKQINKIIDETGVKIDIE 600

Query: 703 GDGTLTILAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLL 762
            DGT+ I + N+++ +K ++ ++  + RE+E+G IY G V  ++++GAFVE   G+ GL+
Sbjct: 601 QDGTIFISSVNEAMNQKAKQIIE-DIVREVEVGQIYLGKVKRIEKFGAFVELFNGKDGLV 660

Query: 763 HISELSHDSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPK 778
           HISEL+ + V RV DVVS+G +I +  +  D +G + LS KA L   K
Sbjct: 661 HISELAEERVGRVEDVVSIGDEILVKVMEIDKQGRVNLSRKAVLRDKK 698

BLAST of Sgr025836 vs. ExPASy Swiss-Prot
Match: A4IME3 (Polyribonucleotide nucleotidyltransferase OS=Geobacillus thermodenitrificans (strain NG80-2) OX=420246 GN=pnp PE=3 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 3.3e-141
Identity = 284/701 (40.51%), Postives = 429/701 (61.20%), Query Frame = 0

Query: 111 EIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQEKQFAQ 170
           ++  R + +ETG++A+ ANGAA++   +T VLST  +++     DF PLTV+Y+E+ +A 
Sbjct: 11  DVAGRPLVIETGELAKQANGAALVRYGDTVVLSTATASREAKDVDFFPLTVNYEERLYAV 70

Query: 171 GVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPDVMA 230
           G IP  F++REG P E+ +L  R+IDRPIRPLF  GF +EVQV++ V+S D    P+V A
Sbjct: 71  GKIPGGFIKREGRPSEKAILASRLIDRPIRPLFAEGFRNEVQVVSMVMSVDQDCSPEVAA 130

Query: 231 ANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTLMID 290
              +S AL +SDIP+ GPI  + +GRI+GQFV+NP ++++  SD++L+ A T++   M++
Sbjct: 131 LIGSSVALTISDIPFEGPIAGVIVGRIDGQFVINPTVEQMEKSDMHLIVAGTKDAINMVE 190

Query: 291 VQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVTKLA 350
             A E+ E+ +   +   H E  + +  Q  +A + GK K E  L         ++ +LA
Sbjct: 191 AGADEVPEEVMLEAIMFGHEEVKRLIAFQEEIAAQVGKEKMEVVLYEPDPELEAEIRQLA 250

Query: 351 EAPIEAVFTDPSYGKFERGEALDKITQDVKRAFE-EESDEESLKVLPKAVDYVRKKVVRR 410
           EA I+     P   K  R  A++ +   V   +E EE+DEE LK + + +  + K+ VRR
Sbjct: 251 EADIKKAVQVPE--KLARDAAIEDVKAGVIAKYEAEEADEEKLKQVQEILHKLVKEEVRR 310

Query: 411 RIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRLES 470
            I  E +R DGR++DE+RPL      LP  HGS +F RG TQVL   TLGA GD Q L+ 
Sbjct: 311 LITVEKIRPDGRKVDEIRPLSSAVGILPRTHGSGLFTRGQTQVLSVCTLGALGDVQILDG 370

Query: 471 L---------------------VGP---PTKR-----TLAEKALLAVLPPENEFPYTVRV 530
           L                      GP   P +R      L E+AL  V+P E EFPYT+R+
Sbjct: 371 LDLEESKRFMHHYNFPPFSVGETGPMRGPGRREIGHGALGERALEPVVPSEREFPYTIRL 430

Query: 531 TSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILTD 590
            SEV+ S+GSTS A++C  ++A+MDAG+P+   VAG+++GLV   D        Y ILTD
Sbjct: 431 VSEVLESNGSTSQASICASTLAMMDAGVPIKAPVAGIAMGLVKNED-------HYTILTD 490

Query: 591 ILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHMERE 650
           I G+EDHLGDMDFK+AGTRKG+TA+Q+DIK  G+  +I+ E+L+ AR GRL+IL+HM + 
Sbjct: 491 IQGIEDHLGDMDFKVAGTRKGVTALQMDIKIKGLTRDILEEALQQARKGRLEILDHMMQT 550

Query: 651 INAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISV-GDGTLTILAK 710
           ++ PR +  K +P++  +    D +R +IGP G    KI +ETG +I +  DGT+ I + 
Sbjct: 551 LSEPRKELSKYAPKILIMHINPDKIREVIGPSGKQINKIIDETGVKIDIEQDGTIFISSV 610

Query: 711 NQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDSV 770
           ++   +K ++ ++  + RE+E+G +Y G V  ++++GAFVE   G+ GL+HISEL+ + +
Sbjct: 611 DEEANQKAKQIIE-DIVREVEVGQVYLGKVKRIEKFGAFVELFNGKDGLVHISELAEERI 670

Query: 771 SRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEG 781
            +V D+VS+G +I +     D +G + LS KA L   +  G
Sbjct: 671 GKVEDIVSIGDEILVKVTEIDKQGRVNLSRKAVLREQRGAG 701

BLAST of Sgr025836 vs. ExPASy TrEMBL
Match: A0A6J1DE40 (Polyribonucleotide nucleotidyltransferase OS=Momordica charantia OX=3673 GN=LOC111019948 PE=3 SV=1)

HSP 1 Score: 1559.7 bits (4037), Expect = 0.0e+00
Identity = 827/943 (87.70%), Postives = 854/943 (90.56%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDPDTAVAG-TKVLETF 108
           MASMA+KANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS Q+LDPDT +A  TKVLETF
Sbjct: 1   MASMASKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQSQQQLDPDTTLASRTKVLETF 60

Query: 109 KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQEK 168
            EEFEIGSRLITLETGKIARFANGAAVLGME+TKVLSTVAS+KGD+VRDFLPLTVDYQEK
Sbjct: 61  TEEFEIGSRLITLETGKIARFANGAAVLGMEDTKVLSTVASSKGDSVRDFLPLTVDYQEK 120

Query: 169 QFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDP 228
           QFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDP
Sbjct: 121 QFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDP 180

Query: 229 DVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKT 288
           DVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNP M+ELNLSDLNLVYACTREKT
Sbjct: 181 DVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPTMNELNLSDLNLVYACTREKT 240

Query: 289 LMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKV 348
           LMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQ+RLA KAGKLKKEYKLSMVSDSTMEKV
Sbjct: 241 LMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQVRLAAKAGKLKKEYKLSMVSDSTMEKV 300

Query: 349 TKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKV 408
           TKLAEAPIEAVFTDPSYGKFERGEAL+ ITQDVKR FEEE DEE LKVLPKAVDYVRKKV
Sbjct: 301 TKLAEAPIEAVFTDPSYGKFERGEALENITQDVKRTFEEECDEEGLKVLPKAVDYVRKKV 360

Query: 409 VRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQR 468
           VRRRIISEGLRVDGRRLDEVRPLYCES YLP+LHGS+IF+RGDTQVLCTVTLGAPGDAQR
Sbjct: 361 VRRRIISEGLRVDGRRLDEVRPLYCESSYLPVLHGSSIFSRGDTQVLCTVTLGAPGDAQR 420

Query: 469 LESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYT 528
           LESLVGPPTKR                             TLAEKALLAVLPPENEFPYT
Sbjct: 421 LESLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPPENEFPYT 480

Query: 529 VRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRI 588
           VR+TSEVMASDGSTSMATVCGGSMALMDAGIPLN+HVAGVSVGLVSE DPSTGAIKDYRI
Sbjct: 481 VRITSEVMASDGSTSMATVCGGSMALMDAGIPLNQHVAGVSVGLVSETDPSTGAIKDYRI 540

Query: 589 LTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHM 648
           LTDILGLEDHLGDMDFKIAGTR+GITAIQLDIKPAGIPL+IICESLEPAR GRLQILEHM
Sbjct: 541 LTDILGLEDHLGDMDFKIAGTREGITAIQLDIKPAGIPLDIICESLEPARKGRLQILEHM 600

Query: 649 EREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTIL 708
           EREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTIL
Sbjct: 601 EREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTIL 660

Query: 709 AKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHD 768
           AKNQSVMEKVQEKVDFTLGREIEIGG+YKGVV SVKEYGAFVEFNGGQQGLLHISELSHD
Sbjct: 661 AKNQSVMEKVQEKVDFTLGREIEIGGVYKGVVASVKEYGAFVEFNGGQQGLLHISELSHD 720

Query: 769 SVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVGE 828
            VSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEG NGN        PPV E
Sbjct: 721 PVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGRNGN------LFPPVSE 780

Query: 829 AFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKDA 888
             K R ETLN  +NTSPVD  V SE N +SSIPSILIRSAEDCDEEEK+S GLNLKLK+ 
Sbjct: 781 VSKMRHETLN--ANTSPVDKGVGSEVNPTSSIPSILIRSAEDCDEEEKRSAGLNLKLKNT 840

Query: 889 QKPNANSRSERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLDLG 948
           +K NA S+S      FTQN VN NEVE   PITA+NLKLGMKVRAKVYQ+R  GLVLDLG
Sbjct: 841 RKLNAMSKS------FTQNDVNMNEVE-SLPITARNLKLGMKVRAKVYQVRVGGLVLDLG 900

Query: 949 EGLRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           EGLRGMYRFE DNRSNFEVGDELHVQCSSF+ KGIP  S V +
Sbjct: 901 EGLRGMYRFEGDNRSNFEVGDELHVQCSSFTVKGIPVMSLVEN 928

BLAST of Sgr025836 vs. ExPASy TrEMBL
Match: A0A6J1EKU5 (Polyribonucleotide nucleotidyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111435245 PE=3 SV=1)

HSP 1 Score: 1543.5 bits (3995), Expect = 0.0e+00
Identity = 815/945 (86.24%), Postives = 857/945 (90.69%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDP-DTAVAGTKVLETF 108
           MASMA+KA+PLFS IPHFLTWRSLGFRT+CCGRMGFASQS Q+LDP DT VA +KVLE+F
Sbjct: 1   MASMASKAHPLFSAIPHFLTWRSLGFRTVCCGRMGFASQSQQQLDPSDTTVARSKVLESF 60

Query: 109 KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAV-RDFLPLTVDYQE 168
           KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTV SAKGDAV  DFLPLTVDYQE
Sbjct: 61  KEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVTSAKGDAVSSDFLPLTVDYQE 120

Query: 169 KQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 228
           KQFAQGVIP+TFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD
Sbjct: 121 KQFAQGVIPSTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 180

Query: 229 PDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREK 288
           P VMAANATSAALMLSDIPWGGPIGVIRIGR++GQFVVNP MDELNLSDLNLVYACTREK
Sbjct: 181 PGVMAANATSAALMLSDIPWGGPIGVIRIGRVDGQFVVNPTMDELNLSDLNLVYACTREK 240

Query: 289 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEK 348
           TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSM+S+STMEK
Sbjct: 241 TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMLSESTMEK 300

Query: 349 VTKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKK 408
           VTKLAEAPIEAVFTD SYGKFERGEALD IT+DVK+ FEEE DEE LKVLPKAVD VRKK
Sbjct: 301 VTKLAEAPIEAVFTDHSYGKFERGEALDNITKDVKKVFEEECDEEGLKVLPKAVDSVRKK 360

Query: 409 VVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQ 468
           VVRRRII+EGLRVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPG+AQ
Sbjct: 361 VVRRRIIAEGLRVDGRRLDEVRPLYCESRYLPILHGSSIFSRGDTQVLCTVTLGAPGEAQ 420

Query: 469 RLESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPY 528
           RL+SLVGPPTKR                             TLAEKALLAVLP E+EFPY
Sbjct: 421 RLDSLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPSEDEFPY 480

Query: 529 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYR 588
           TVRVTSEVMASDGSTSMATVCGGSMALMDAGIP+N HVAGVSVGLVSE DP+TGAI DYR
Sbjct: 481 TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPINAHVAGVSVGLVSETDPTTGAIIDYR 540

Query: 589 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEH 648
           ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GRLQILEH
Sbjct: 541 ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRLQILEH 600

Query: 649 MEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTI 708
           MEREINAPRIKDD+NSPRL  LKYTNDALRRLIGPLGA KKKIEEETGARISVGDGTLTI
Sbjct: 601 MEREINAPRIKDDENSPRLVILKYTNDALRRLIGPLGASKKKIEEETGARISVGDGTLTI 660

Query: 709 LAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 768
           LAKNQ+VMEKVQEKVDFTLGREIEIGG+YKGVVTSVKEYGAFVEFNGGQQGLLHISELSH
Sbjct: 661 LAKNQAVMEKVQEKVDFTLGREIEIGGMYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 720

Query: 769 DSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVG 828
           D VSRVSDVVSVGQ+ISL+CIGQDVRGNIKLSLKAT+P PKAE  NGNSVPS + V PVG
Sbjct: 721 DPVSRVSDVVSVGQRISLVCIGQDVRGNIKLSLKATMPRPKAEAQNGNSVPSLDPVSPVG 780

Query: 829 EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKD 888
           E  KT  ET NSTSNTSPVD  V SE N +SS+PSILIRSAEDCDEEEKKS  +N+KLKD
Sbjct: 781 EVSKTLHETPNSTSNTSPVDISVGSEVNSTSSVPSILIRSAEDCDEEEKKSADMNVKLKD 840

Query: 889 AQKPNANSRSERKSKPFTQN-GVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLD 948
            +K NANSRSERK KP  QN G + NEVEV+SPITA+NLKLG++VRAKVYQIRARGLVLD
Sbjct: 841 TRKSNANSRSERKLKPLAQNGGPSNNEVEVKSPITARNLKLGVRVRAKVYQIRARGLVLD 900

Query: 949 LGEGLRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           LGEGLRGMY+FE DNRSNFEVGDELHVQCSSFS+KGIP  S V +
Sbjct: 901 LGEGLRGMYKFEGDNRSNFEVGDELHVQCSSFSTKGIPVMSLVDN 945

BLAST of Sgr025836 vs. ExPASy TrEMBL
Match: A0A6J1JPN1 (Polyribonucleotide nucleotidyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486364 PE=3 SV=1)

HSP 1 Score: 1536.9 bits (3978), Expect = 0.0e+00
Identity = 809/941 (85.97%), Postives = 853/941 (90.65%), Query Frame = 0

Query: 52  MATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDPDTAVAG-TKVLETFKEE 111
           MA+KA+PLFS IPHFLTWRSL FRT+CCGRMGFASQS Q+ DP  A++G TKVLE+FKEE
Sbjct: 1   MASKAHPLFSAIPHFLTWRSLSFRTVCCGRMGFASQSQQQRDPTDAISGRTKVLESFKEE 60

Query: 112 FEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAV-RDFLPLTVDYQEKQF 171
           FEIGSRLITLETGKIARFANGAAVLGMEETKVLSTV SAKGDAV  DFLPLTVDYQEKQF
Sbjct: 61  FEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVTSAKGDAVCSDFLPLTVDYQEKQF 120

Query: 172 AQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPDV 231
           AQGVIP+TFMRREGAPKERELLCGRIIDRPIRPLFP+GFYHEVQVMASVLSSDGKQDP V
Sbjct: 121 AQGVIPSTFMRREGAPKERELLCGRIIDRPIRPLFPSGFYHEVQVMASVLSSDGKQDPGV 180

Query: 232 MAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTLM 291
           MAANATSAALMLSDIPWGGPIGVIRIGR++GQFVVNP MDELNLSDLNLVYACTREKTLM
Sbjct: 181 MAANATSAALMLSDIPWGGPIGVIRIGRVDGQFVVNPTMDELNLSDLNLVYACTREKTLM 240

Query: 292 IDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVTK 351
           IDVQA EITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSM+SDSTMEKVTK
Sbjct: 241 IDVQACEITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMLSDSTMEKVTK 300

Query: 352 LAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKVVR 411
           LAEAPIEAVFTD SYGKFERGEALDKIT+DV++ FEEE DEE LKVLPKAVD VRKKVVR
Sbjct: 301 LAEAPIEAVFTDHSYGKFERGEALDKITKDVEKVFEEECDEEGLKVLPKAVDRVRKKVVR 360

Query: 412 RRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRLE 471
           RRII+EGLRVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPG+AQRL+
Sbjct: 361 RRIIAEGLRVDGRRLDEVRPLYCESRYLPILHGSSIFSRGDTQVLCTVTLGAPGEAQRLD 420

Query: 472 SLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYTVR 531
           SLVGPPTKR                             TLAEKALLAVLP E+EFPYTVR
Sbjct: 421 SLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPSEDEFPYTVR 480

Query: 532 VTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILT 591
           VTSEVMASDGSTSMATVCGGSMALMDAGIP+N HVAGVSVGLVSE DP+TGAI DYRILT
Sbjct: 481 VTSEVMASDGSTSMATVCGGSMALMDAGIPINAHVAGVSVGLVSETDPTTGAIIDYRILT 540

Query: 592 DILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHMER 651
           DILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GRLQILEHME 
Sbjct: 541 DILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRLQILEHMES 600

Query: 652 EINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTILAK 711
           EINAPRIKDD NSPRL  LKYTNDALRRLIGPLGA KKKIEEETGARISVGDGTLTILAK
Sbjct: 601 EINAPRIKDDANSPRLVILKYTNDALRRLIGPLGASKKKIEEETGARISVGDGTLTILAK 660

Query: 712 NQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDSV 771
           NQ+VMEKVQEKVDFTLGREIEIGG+YKGVVTSVKEYGAFVEFNGGQQGLLHISELSHD V
Sbjct: 661 NQAVMEKVQEKVDFTLGREIEIGGMYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDPV 720

Query: 772 SRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVGEAF 831
           SRVSDVVSVGQ+ISL+CIGQDVRGNIKLSLKAT+P PKAE  NGNSVPS + V PVGE  
Sbjct: 721 SRVSDVVSVGQRISLVCIGQDVRGNIKLSLKATMPRPKAEAQNGNSVPSLDPVSPVGEVP 780

Query: 832 KTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKDAQK 891
           KT  ET NSTSNTSPVDT V SE NR+SSIPSILIRSAEDCDEE+KKS  +N+KLKD +K
Sbjct: 781 KTWHETPNSTSNTSPVDTSVGSEVNRTSSIPSILIRSAEDCDEEDKKSADMNVKLKDTRK 840

Query: 892 PNANSRSERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLDLGEG 951
            NANSRSERKSKP  QNG + NEV+V+SPI A+NLKLG++VRAKVYQIRARGLVLDLGEG
Sbjct: 841 SNANSRSERKSKPLAQNGPSNNEVDVESPINARNLKLGVRVRAKVYQIRARGLVLDLGEG 900

Query: 952 LRGMYRFEEDNRSNFEVGDELHVQCSSFSSKGIPEQSFVSS 961
           LRGMY+FE DNRSNFEVGDELHVQCSSFSSKGIP  S V++
Sbjct: 901 LRGMYKFEGDNRSNFEVGDELHVQCSSFSSKGIPVMSLVAN 941

BLAST of Sgr025836 vs. ExPASy TrEMBL
Match: A0A0A0LFJ2 (Polyribonucleotide nucleotidyltransferase OS=Cucumis sativus OX=3659 GN=Csa_3G838680 PE=3 SV=1)

HSP 1 Score: 1466.8 bits (3796), Expect = 0.0e+00
Identity = 780/1032 (75.58%), Postives = 839/1032 (81.30%), Query Frame = 0

Query: 49   MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS-QRLDPDTAVAGTKVLETFK 108
            MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGF+SQS Q++DP+T +  TKVLETF+
Sbjct: 1    MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFSSQSQQQVDPNTTLGRTKVLETFE 60

Query: 109  EEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQEKQ 168
            E FEIGSRL+ LETGKIARFANGAAVLG+EETKVLSTVASAKGDA RDFLPLTVDYQEKQ
Sbjct: 61   EVFEIGSRLVKLETGKIARFANGAAVLGIEETKVLSTVASAKGDAARDFLPLTVDYQEKQ 120

Query: 169  FAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPD 228
            FAQGVIP TF RREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPD
Sbjct: 121  FAQGVIPGTFTRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPD 180

Query: 229  VMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTL 288
            VMAANATSAALMLSDIPWGGPIGVIRIGRI+GQFVVNP MDELNLSDLNL+YACTREKTL
Sbjct: 181  VMAANATSAALMLSDIPWGGPIGVIRIGRIDGQFVVNPTMDELNLSDLNLIYACTREKTL 240

Query: 289  MIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVT 348
            MIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSMVSDST+EKV 
Sbjct: 241  MIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMVSDSTLEKVA 300

Query: 349  KLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKVV 408
            KLAE+PIEAVFTDPSYGKFERGEAL+KIT+DVK+ FEEE  EE LKVLPKAVDYVRKKVV
Sbjct: 301  KLAESPIEAVFTDPSYGKFERGEALEKITEDVKKVFEEECYEEGLKVLPKAVDYVRKKVV 360

Query: 409  RRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRL 468
            RRRII+EG R+DGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAP DAQ L
Sbjct: 361  RRRIIAEGRRLDGRRLDEVRPLYCESSYLPILHGSSIFSRGDTQVLCTVTLGAPADAQHL 420

Query: 469  ESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYTV 528
            +SLVGPPTKR                             TLAEKALLAVLPPE+EFPY V
Sbjct: 421  DSLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPPESEFPYAV 480

Query: 529  RVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRIL 588
            R+ SEVMASDGSTSMATVCGGSMALMDAG+PLNEHVAGVSVGLVSE DPSTGAIKDYRIL
Sbjct: 481  RINSEVMASDGSTSMATVCGGSMALMDAGVPLNEHVAGVSVGLVSETDPSTGAIKDYRIL 540

Query: 589  TDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHME 648
            TDILGLEDHLGDMDFKIAGTRKG+TAIQLDIKPAGIPL+IICESLE AR GR QIL+HME
Sbjct: 541  TDILGLEDHLGDMDFKIAGTRKGVTAIQLDIKPAGIPLDIICESLEHARKGRWQILDHME 600

Query: 649  REINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTILA 708
            REINAPR+KDD+N PRL TLKYTN+ALRRLIGPLG +KKKIE ETGARISVGDGTLTILA
Sbjct: 601  REINAPRMKDDENCPRLVTLKYTNEALRRLIGPLGIVKKKIEAETGARISVGDGTLTILA 660

Query: 709  KNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDS 768
            KNQ+VME VQ++VDFTLGREIEIGG YKGVV+SVKEYGAF+EFNGGQQGLLHISELSHD 
Sbjct: 661  KNQAVMENVQDRVDFTLGREIEIGGTYKGVVSSVKEYGAFIEFNGGQQGLLHISELSHDP 720

Query: 769  VSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVGEA 828
            VSRVSD+VSVGQKISL CIGQDV GNIKLSLKA LP PKA+G NGNSV S ES+P VGE 
Sbjct: 721  VSRVSDIVSVGQKISLRCIGQDVHGNIKLSLKALLPVPKAKGPNGNSVSSLESLPSVGEV 780

Query: 829  FKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCD----------------- 888
            +KT+P+T NSTSN   VD  V SE N +SS+PS+LIRSAEDCD                 
Sbjct: 781  YKTQPKTQNSTSNIPAVDKGVGSEVNPTSSVPSVLIRSAEDCDVEEKKSAVKNLKLKNTQ 840

Query: 889  ------------------------------------------------------------ 948
                                                                        
Sbjct: 841  KLNNASTSDEDCDEEEKQSAVNNRKQKNTRKLKTTSKSDENCDEEEKKSAVKKPKRKTTQ 900

Query: 949  -------------EEEKKSTGLNLKLKDAQKPNANSRSERKSKPFTQNGVNTNEVEVQSP 961
                         EEEKKS   NLKLK  +K NA SRS+ +SKP TQN VN NEVEVQ P
Sbjct: 901  KQNTASTSAEDCNEEEKKSAVGNLKLKSTKKLNATSRSKGQSKPLTQNDVNNNEVEVQDP 960

BLAST of Sgr025836 vs. ExPASy TrEMBL
Match: A0A5A7UEM0 (Polyribonucleotide nucleotidyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1545G00220 PE=3 SV=1)

HSP 1 Score: 1448.3 bits (3748), Expect = 0.0e+00
Identity = 775/1034 (74.95%), Postives = 830/1034 (80.27%), Query Frame = 0

Query: 49   MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQS---QRLDPDTAVAGTKVLET 108
            MASMA+KANPLFSTIPHFLTWRS GFRTICCGRMGFASQS   Q+LDPDT + GTKVLET
Sbjct: 1    MASMASKANPLFSTIPHFLTWRSHGFRTICCGRMGFASQSQSQQQLDPDTTLGGTKVLET 60

Query: 109  FKEEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQE 168
            F+E FEIGSRL+ LETGKIARFANGAAVLG+EETKVLSTVASAKG AVRDFLPLTVDYQE
Sbjct: 61   FEEVFEIGSRLVKLETGKIARFANGAAVLGIEETKVLSTVASAKGGAVRDFLPLTVDYQE 120

Query: 169  KQFAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQD 228
            KQFAQGVIP TF RREGAPKERELLCGRIIDRPIRPLFP GFYHEVQVMASVLSSDGKQD
Sbjct: 121  KQFAQGVIPGTFTRREGAPKERELLCGRIIDRPIRPLFPTGFYHEVQVMASVLSSDGKQD 180

Query: 229  PDVMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREK 288
            PDVMAANATSAALMLSDIPWGGPIGVIRIGRI+GQFVVNP MDELNLSDLNL+YACTREK
Sbjct: 181  PDVMAANATSAALMLSDIPWGGPIGVIRIGRIDGQFVVNPTMDELNLSDLNLIYACTREK 240

Query: 289  TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEK 348
            TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLA KAGKLKKEYKLSMVSDST+EK
Sbjct: 241  TLMIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLAAKAGKLKKEYKLSMVSDSTLEK 300

Query: 349  VTKLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKK 408
            VTKLAE PIEAVFTDPSYGKFERGEAL+KIT+DVK+ FEEE DEE LKVLPKAVDYVRKK
Sbjct: 301  VTKLAEFPIEAVFTDPSYGKFERGEALEKITEDVKKVFEEECDEEGLKVLPKAVDYVRKK 360

Query: 409  VVRRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQ 468
            VVRRRII+EG RVDGRRLDEVRPLYCES YLPILHGS+IF+RGDTQVLCTVTLGAPGDAQ
Sbjct: 361  VVRRRIIAEGRRVDGRRLDEVRPLYCESSYLPILHGSSIFSRGDTQVLCTVTLGAPGDAQ 420

Query: 469  RLESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPY 528
             L+SLVGPPTKR                             TLAEKALLAVLPPE+EFPY
Sbjct: 421  HLDSLVGPPTKRFMLHYSFPPFSINEVGKRGGLNRREVGHGTLAEKALLAVLPPESEFPY 480

Query: 529  TVRVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYR 588
             VR+ SEVMASDGSTSMATVCGGSMALMDAG+PLNEHVAGVSVGLVSE DPSTG IKDYR
Sbjct: 481  AVRINSEVMASDGSTSMATVCGGSMALMDAGVPLNEHVAGVSVGLVSETDPSTGEIKDYR 540

Query: 589  ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEH 648
            ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPL+IICESLE AR GR QIL+H
Sbjct: 541  ILTDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLDIICESLEHARKGRWQILDH 600

Query: 649  MEREINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTI 708
            MEREINAPR+KDD+N PRL TLKYTNDALRRLIGPLG LK+KIE ETGARIS+GDGTLTI
Sbjct: 601  MEREINAPRMKDDENCPRLVTLKYTNDALRRLIGPLGILKRKIEAETGARISIGDGTLTI 660

Query: 709  LAKNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSH 768
            LAKNQ+VME VQ++VDFTLGR+IEIGG YKGVVTSVKEYGAF+EFNGGQQGLLHISELSH
Sbjct: 661  LAKNQAVMENVQDRVDFTLGRQIEIGGTYKGVVTSVKEYGAFIEFNGGQQGLLHISELSH 720

Query: 769  DSVSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVG 828
            D VSR+SDVVSVGQ+I+L CIGQDV GNIKLSLKA LP PKA+G NGNSV SSE +P   
Sbjct: 721  DPVSRISDVVSVGQRITLRCIGQDVHGNIKLSLKALLPVPKAKGPNGNSVSSSEPLP--- 780

Query: 829  EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEKKSTGLNLKLKD 888
                 + +T NSTSN  PV+  V SE   +SS+PS+LIRSAE+CD EEK+S   NLKLK+
Sbjct: 781  -----QQKTQNSTSNIPPVEKGVGSEVKPTSSVPSVLIRSAEECDVEEKQSAVTNLKLKN 840

Query: 889  AQKP-------------------------------------------------------- 948
             QKP                                                        
Sbjct: 841  TQKPNNASTSAEDSDEKEKQSAVSNRKQKNTRKLKTTSKNAENCDEEEKKSADKKPKRKT 900

Query: 949  ----------------------------------NANSRSERKSKPFTQNGVNTNEVEVQ 961
                                              N  SRS+ +SKP TQN VN NEVEV+
Sbjct: 901  TQKLNAASISAEDCNEEEKKSDIGNLELKTTKKLNTPSRSKGQSKPLTQNDVNNNEVEVK 960

BLAST of Sgr025836 vs. TAIR 10
Match: AT5G14580.1 (polyribonucleotide nucleotidyltransferase, putative )

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 616/999 (61.66%), Postives = 749/999 (74.97%), Query Frame = 0

Query: 49  MASMATKANPLFSTIPHFLTWRSLGFRTICCGRMGFASQSQRLDPDTAV-AGTKVLETFK 108
           M+S+  +A+   +++P+FL WR+LGFRTIC GR+GFA       PD+ V AGTK+LE+FK
Sbjct: 1   MSSIVNRASS--ASLPNFLAWRALGFRTICSGRLGFAPSV----PDSPVSAGTKILESFK 60

Query: 109 EEFEIGSRLITLETGKIARFANGAAVLGMEETKVLSTVASAKGDAVRDFLPLTVDYQEKQ 168
           EEFE+GSR+++ ETGKIARFANG+ VLGM+ETKVLSTV  AK D+ RDFLPLTVDYQEKQ
Sbjct: 61  EEFEVGSRVVSFETGKIARFANGSVVLGMDETKVLSTVTCAKTDSPRDFLPLTVDYQEKQ 120

Query: 169 FAQGVIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPD 228
           +AQG+IPNT+MRREGAPKERELLCGR+IDRPIRPLFP GFYHEVQ+MASVLSSDGKQDPD
Sbjct: 121 YAQGLIPNTYMRREGAPKERELLCGRLIDRPIRPLFPTGFYHEVQIMASVLSSDGKQDPD 180

Query: 229 VMAANATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTL 288
           ++AANA+SAALMLSD+PWGGPIGVIRIGRI GQFVVNP MDEL+ SDLNL+YACTR+KT+
Sbjct: 181 ILAANASSAALMLSDVPWGGPIGVIRIGRICGQFVVNPTMDELSSSDLNLIYACTRDKTM 240

Query: 289 MIDVQAREITEKDLEAGLRLAHPEAVKFLEPQIRLADKAGKLKKEYKLSMVSDSTMEKVT 348
           MIDVQ+REI+EKDL A LRLAHPEAVK+L+PQIRLA+KAGK KKEYKLSM+SD T+EKV 
Sbjct: 241 MIDVQSREISEKDLAAALRLAHPEAVKYLDPQIRLAEKAGKQKKEYKLSMLSDKTLEKVA 300

Query: 349 KLAEAPIEAVFTDPSYGKFERGEALDKITQDVKRAFEEESDEESLKVLPKAVDYVRKKVV 408
            LA   IE+VFTDPSYGKFERGEALD I +DV++ FEEE D+ESL +LPKAVD VRKKVV
Sbjct: 301 DLAATRIESVFTDPSYGKFERGEALDNIGKDVRKVFEEEGDQESLSILPKAVDTVRKKVV 360

Query: 409 RRRIISEGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRL 468
           R R+IS+G RVDGR +DEVRP+YCES YLP LHGSA+F+RGDTQVLCTVTLGAP +AQ L
Sbjct: 361 RSRMISDGFRVDGRHVDEVRPIYCESHYLPALHGSALFSRGDTQVLCTVTLGAPAEAQSL 420

Query: 469 ESLVGPPTKR-----------------------------TLAEKALLAVLPPENEFPYTV 528
           +SLVGPP KR                             TLAEKALLAVLPPE  FPYT+
Sbjct: 421 DSLVGPPKKRFMLHYSFPPYCTNEVGKRGGLNRREVGHGTLAEKALLAVLPPEEAFPYTI 480

Query: 529 RVTSEVMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRIL 588
           R+ SEVM+SDGSTSMA+VCGGSMALMDAGIPL  HVAGVSVGL++++DPS+G IKDYRI+
Sbjct: 481 RINSEVMSSDGSTSMASVCGGSMALMDAGIPLRAHVAGVSVGLITDVDPSSGEIKDYRIV 540

Query: 589 TDILGLEDHLGDMDFKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHME 648
           TDILGLEDHLGDMDFKIAGTR G+TAIQLDIKPAGIPL+I+CESLE AR  RLQIL+HME
Sbjct: 541 TDILGLEDHLGDMDFKIAGTRDGVTAIQLDIKPAGIPLDIVCESLENAREARLQILDHME 600

Query: 649 REINAPRIKDDKNSPRLATLKYTNDALRRLIGPLGALKKKIEEETGARISVGDGTLTILA 708
           R IN+PR +D   SPRLATLKY+ND+LR LIGP+G LK+KIE ETGAR+S+ +GTLTI+A
Sbjct: 601 RNINSPRGQDGAYSPRLATLKYSNDSLRTLIGPMGVLKRKIEVETGARLSIDNGTLTIVA 660

Query: 709 KNQSVMEKVQEKVDFTLGREIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDS 768
           KNQ VMEK QE+VDF +GRE+ +GG+YKG V+S+KEYGAFVEF GGQQGLLH+SELSH+ 
Sbjct: 661 KNQDVMEKAQEQVDFIIGRELVVGGVYKGTVSSIKEYGAFVEFPGGQQGLLHMSELSHEP 720

Query: 769 VSRVSDVVSVGQKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSES--VPPVG 828
           VS+VSDV+ +GQ I+ MCI  DVRGNIKLS KA LP PK +       P+S++   P + 
Sbjct: 721 VSKVSDVLDIGQCITTMCIETDVRGNIKLSRKALLPKPKRK-------PASDAGKDPVMK 780

Query: 829 EAFKTRPETLNSTSNTSPVDTIVASEANRSSSIPSILIRSAEDCDEEEK----------- 888
           E+     E  +     + + +IV        S+P+++IR+A +C+E EK           
Sbjct: 781 ESSTVYIENSSVGEIVASMPSIVTPLQKSRLSVPAVVIRTAVECNEAEKSSPVNDNDKPR 840

Query: 889 -----------KSTGLNL------------------------------KLKDAQKPNANS 948
                      KST   L                              KLK     N ++
Sbjct: 841 RAATSKPDRKPKSTASKLIATQKEEEALESIAPEETSAECGEILKQDGKLKSVSPKNNST 900

Query: 949 RS-----ERKSKPFTQNGVNTNEVEVQSPITAKNLKLGMKVRAKVYQIRARGLVLDLGEG 959
            S      +  K   +  ++ N+ E  + ++ + LK+G ++ A V  +RA GLVLDLG  
Sbjct: 901 ASNLVSFSKAKKSTMKENLSENKAEESASVSTRKLKIGTEMTATVDHVRALGLVLDLGGE 960

BLAST of Sgr025836 vs. TAIR 10
Match: AT3G03710.1 (polyribonucleotide nucleotidyltransferase, putative )

HSP 1 Score: 369.4 bits (947), Expect = 9.0e-102
Identity = 276/804 (34.33%), Postives = 410/804 (51.00%), Query Frame = 0

Query: 113 GSRLITLETGKIARFANGAAVLGMEETKVLSTVASAK-GDAVRDFLPLTVDYQEKQFAQG 172
           G+R I +ETG + R A+ A  +   ET V ++V  A       DFLPL V YQE+  A G
Sbjct: 95  GNREILVETGLMGRQASSAVTVTDGETIVYTSVCLADVPSEPSDFLPLYVHYQERFSAVG 154

Query: 173 VIPNTFMRREGAPKERELLCGRIIDRPIRPLFPAGFYHEVQVMASVLSSDGKQDPDVMAA 232
                F +REG  K+ E+L  R+IDRP+RP  P GFY+E Q+++ VLS DG   PD +A 
Sbjct: 155 RTSGGFFKREGRTKDHEVLICRLIDRPLRPTMPKGFYNETQILSWVLSYDGLHAPDALAV 214

Query: 233 NATSAALMLSDIPWGGPIGVIRIGRINGQFVVNPAMDELNLSDLNLVYACTREKTLMID- 292
            +   A+ LS++P    I  +R+G I G+F+VNP + E+  S L+L  A T    L I+ 
Sbjct: 215 TSAGIAVALSEVPNAKAIAGVRVGLIGGEFIVNPTVKEMEESQLDLFLAGTDTAILTIEG 274

Query: 293 ----------VQAREITEKDLEA-------------------GLRLAHPEAVKFL-EPQI 352
                     +QA ++ +  ++A                    +RL  PE  K + E   
Sbjct: 275 YSNFLPEEMLLQAVKVGQDAVQATCIAIEVLAKKYGKPKMLDAIRLPPPELYKHVKELAG 334

Query: 353 RLADKAGKLKKEYKLSMVSDSTMEKV-TKLAEAPIEAVFTDPSYGKFE-RGEALDKITQD 412
               KA ++K +        S  EKV T L E     V  + ++G  E + + L+   +D
Sbjct: 335 EELTKALQIKSKISRRKAISSLEEKVLTILTEKGY--VIDEVAFGTIEAQPDLLEDEDED 394

Query: 413 VKRAFEEESDEESLKVLP----------KAVDY------VRKKVVRRRIISEGLRVDGRR 472
            +   E E D+  + + P            VD       V  K++RRRI+  G R DGR 
Sbjct: 395 EEVVPEGEVDQGDVHIRPIPRKPIPLLFSEVDVKLVFKEVSSKLLRRRIVEGGKRSDGRT 454

Query: 473 LDEVRPLYCESCYLPILHGSAIFNRGDTQVLCTVTLGAPGDAQRLESL------------ 532
           LDE+RP+      LP  HGS +F RG+TQ L  VTLG    AQR+++L            
Sbjct: 455 LDEIRPINSRCGLLPRAHGSTLFTRGETQALAVVTLGDKQMAQRIDNLEGSDEYKRFYLQ 514

Query: 533 -------------VGPPTKR-----TLAEKALLAVLPPENEFPYTVRVTSEVMASDGSTS 592
                        +G P++R     TLAE+AL  +LP +++FPYT+RV S V+ S+GS+S
Sbjct: 515 YTFPPSSVGEVGRIGAPSRREIGHGTLAERALETILPSDDDFPYTIRVESTVIESNGSSS 574

Query: 593 MATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILTDILGLEDHLGDMD 652
           MA+VCGG +AL DAG+P+   VAG+++G+V + +   G      IL+DI G ED  GDMD
Sbjct: 575 MASVCGGCLALQDAGVPVKCSVAGIAMGMVWDTE-EFGGDGSPLILSDITGAEDASGDMD 634

Query: 653 FKIAGTRKGITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHMEREINAPRIKDDKNS 712
           FK+AG   G+TA Q+DIK  GI LEI+ ++L  A+ GR  IL  M +    P +   K +
Sbjct: 635 FKVAGNEDGVTAFQMDIKVGGITLEIMEKALIQAKAGRRHILAEMAKCSPPPTLSLSKYA 694

Query: 713 PRLATLKYTNDALRRLIGPLGALKKKIEEETGARI--SVGDGTLTILAKNQSVMEKVQEK 772
           P +  +K     +  LIG  G   K I EE+G        DGT+ I+A + + +E+ +  
Sbjct: 695 PLILIMKVHPSKVYSLIGSGGKKVKSIIEESGVEAIDMQDDGTVKIMAIDVASLERAKAI 754

Query: 773 VDFTLGREIEIGGIYKGV-VTSVKEYGAFVEFNGGQQGLLHISELSHDSVSRVSDVVSVG 832
           +   L     +G IY+   + S+  YGAFVE   G++GL HISELS + +++  D   VG
Sbjct: 755 IS-GLTMVPSVGDIYRNCEIKSMAPYGAFVEIAPGREGLCHISELSAEWLAKPEDAYKVG 814

Query: 833 QKISLMCIGQDVRGNIKLSLKATLPGPKAEGWNGNSVPSSESVPPVGEAFKTRPETLNST 834
            +I +  I  + +G ++LS++A LP  + +         S+   P G++ K +  +    
Sbjct: 815 DRIDVKLIEVNEKGQLRLSVRALLPESETD-------KDSQKQQPAGDSTKDK-SSQRKY 874

BLAST of Sgr025836 vs. TAIR 10
Match: AT5G30510.1 (ribosomal protein S1 )

HSP 1 Score: 65.9 bits (159), Expect = 2.1e-10
Identity = 34/80 (42.50%), Postives = 50/80 (62.50%), Query Frame = 0

Query: 698 EIEIGGIYKGVVTSVKEYGAFVEFNGGQQGLLHISELSHDSVSRVSDVVSVGQKISLMCI 757
           ++ IG +  GVV S+K YGAF++  GG  GLLH+S++SHD VS ++ V+  G  + +M +
Sbjct: 260 QLGIGSVVLGVVQSLKPYGAFIDI-GGINGLLHVSQISHDRVSDIATVLQPGDTLKVMIL 319

Query: 758 GQD-VRGNIKLSLKATLPGP 777
             D  RG + LS K   P P
Sbjct: 320 SHDRDRGRVSLSTKKLEPTP 338

BLAST of Sgr025836 vs. TAIR 10
Match: AT3G61620.1 (3'-5'-exoribonuclease family protein )

HSP 1 Score: 57.8 bits (138), Expect = 5.8e-08
Identity = 61/240 (25.42%), Postives = 100/240 (41.67%), Query Frame = 0

Query: 414 EGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQ---------------------- 473
           EGLR+DGRR +E+R +  E   +    GSA+F  G+T+                      
Sbjct: 7   EGLRLDGRRFNEMRQIVAEVGVVSKADGSAVFEMGNTKVIAAVYGPREIQNKSQQKKNDH 66

Query: 474 --VLCTVTLG--APGDAQRLE----SLVGPPTKRTLAEKALLAVLPPENEFPYTVRVTSE 533
             VLC  ++   + GD +R +    S       R   E  +L  L P ++    + +  +
Sbjct: 67  AVVLCEYSMAQFSTGDRRRQKFDRRSTELSLVIRQTMEACILTELMPHSQ----IDIFLQ 126

Query: 534 VMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILTDILG 593
           V+ +DG T  A +   ++AL DAGIP+ +     S G ++              L D+  
Sbjct: 127 VLQADGGTRSACINAATLALADAGIPMRDLAVSCSAGYLNSTP-----------LLDLNY 186

Query: 594 LEDHLGDMDFKIAGTRK--GITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHMEREI 622
           +ED  G  D  +    K   ++ +Q+D K   +P+E        A  G   I E + RE+
Sbjct: 187 VEDSAGGADVTVGILPKLDKVSLLQMDAK---LPMETFETVFALASEGCKAIAERI-REV 227

BLAST of Sgr025836 vs. TAIR 10
Match: AT3G61620.2 (3'-5'-exoribonuclease family protein )

HSP 1 Score: 57.8 bits (138), Expect = 5.8e-08
Identity = 61/240 (25.42%), Postives = 100/240 (41.67%), Query Frame = 0

Query: 414 EGLRVDGRRLDEVRPLYCESCYLPILHGSAIFNRGDTQ---------------------- 473
           EGLR+DGRR +E+R +  E   +    GSA+F  G+T+                      
Sbjct: 7   EGLRLDGRRFNEMRQIVAEVGVVSKADGSAVFEMGNTKVIAAVYGPREIQNKSQQKKNDH 66

Query: 474 --VLCTVTLG--APGDAQRLE----SLVGPPTKRTLAEKALLAVLPPENEFPYTVRVTSE 533
             VLC  ++   + GD +R +    S       R   E  +L  L P ++    + +  +
Sbjct: 67  AVVLCEYSMAQFSTGDRRRQKFDRRSTELSLVIRQTMEACILTELMPHSQ----IDIFLQ 126

Query: 534 VMASDGSTSMATVCGGSMALMDAGIPLNEHVAGVSVGLVSELDPSTGAIKDYRILTDILG 593
           V+ +DG T  A +   ++AL DAGIP+ +     S G ++              L D+  
Sbjct: 127 VLQADGGTRSACINAATLALADAGIPMRDLAVSCSAGYLNSTP-----------LLDLNY 186

Query: 594 LEDHLGDMDFKIAGTRK--GITAIQLDIKPAGIPLEIICESLEPARNGRLQILEHMEREI 622
           +ED  G  D  +    K   ++ +Q+D K   +P+E        A  G   I E + RE+
Sbjct: 187 VEDSAGGADVTVGILPKLDKVSLLQMDAK---LPMETFETVFALASEGCKAIAERI-REV 227