Sgr025714 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025714 vs. NCBI nr
Match: KAG6574794.1 (hypothetical protein SDJN03_25433, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 748.0 bits (1930), Expect = 6.0e-212
Identity = 396/672 (58.93%), Postives = 447/672 (66.52%), Query Frame = 0

Query: 61  MEGGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPGASEKL 120
           ME GAKGKV VTGGTGFI SWL+K LLE GYSVTTTVR+DPE +KDY FLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTNLPGASEKL 60

Query: 121 QIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNSK 180
           QIY ADLD+ NSFAPAI GCIGVFH+ATP+D  DKE VEV  RR+I+GTLGILK CL+SK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 181 --------------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 240
                                      CWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 180

Query: 241 QEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILG----------------------- 300
           Q++GLEVVT+LPTYV+GPFICPRLP S+  T +L+ G                       
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 301 -------------------ISL-------------------------------------- 360
                              I+L                                      
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 361 ----------------------------------------RFARWVF------------- 420
                                                   R  RW+              
Sbjct: 301 LDAGFQYKYAPHDCKKRPIICGLLYEMVGAANSEDSKPWKRDGRWLLCHYCHKTQLRNRE 360

Query: 421 --DEAKGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKL 480
             +  KGKVCVTGGTGF+ SWL+K LLEDGYSVTTTVR DPEK KDY FLT+LP ASEKL
Sbjct: 361 MENLEKGKVCVTGGTGFLASWLIKSLLEDGYSVTTTVRADPEKNKDYGFLTSLPNASEKL 420

Query: 481 QIYRADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSK 540
           QIY ADLNDPNSFAPAI GCIGVFH+ATPID +D+E  E + RR I+GTLGILK CL+SK
Sbjct: 421 QIYHADLNDPNSFAPAIAGCIGVFHLATPIDEKDEEDVEVLTRRAIEGTLGILKICLDSK 480

Query: 541 TVRRVVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFS 572
           TVRRVV+ SSV T+ F+++ VD+LDESCWSDIDYIN+ +PFGRSYP SKTLTE+AVL+FS
Sbjct: 481 TVRRVVHASSVVTMDFNHHTVDVLDESCWSDIDYINSASPFGRSYPTSKTLTEKAVLEFS 540

BLAST of Sgr025714 vs. NCBI nr
Match: TKY68150.1 (Vestitone reductase [Spatholobus suberectus])

HSP 1 Score: 636.7 bits (1641), Expect = 1.9e-178
Identity = 339/654 (51.83%), Postives = 424/654 (64.83%), Query Frame = 0

Query: 61  MEGGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDP-EKKKDYSFLTNLPGASEK 120
           ME G KG+V VTGGTGFIGSW+VKRLLEDGYSV TTVR DP E KKD SFLT+LPGAS++
Sbjct: 1   MEHG-KGRVCVTGGTGFIGSWIVKRLLEDGYSVNTTVRPDPVEHKKDVSFLTSLPGASQR 60

Query: 121 LQIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNS 180
           LQI+ ADL +  SF+ AIEGC+GVFHVATPVD E KEP EV  +RSIDGTLGILKACLNS
Sbjct: 61  LQIFSADLSNPESFSAAIEGCVGVFHVATPVDFELKEPEEVVTKRSIDGTLGILKACLNS 120

Query: 181 K-------------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 240
           K                           WSD+D +    P G SY +SKTLTE++VLEF 
Sbjct: 121 KTVKRVVYTSSASALNSNGKGEMVDESSWSDVDDLRASKPHGWSYAVSKTLTEKSVLEFG 180

Query: 241 QEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILG----------------------- 300
           +++GL+VVT+LPT V GPFICP++P S+   +  I                         
Sbjct: 181 EQNGLDVVTLLPTSVFGPFICPQIPISIINILKEIYAGEKSAFGFMLQTPMVHVDDVARA 240

Query: 301 -----------------------------ISLRF------------------------AR 360
                                        +S ++                        + 
Sbjct: 241 HIFLLEHPNPKGRYNCSQCLVTFERISELVSAKYPEFQLETQTEGMKVPDLSSKKLTDSG 300

Query: 361 WVF-------------------------DEAKGKVCVTGGTGFIGSWLVKRLLEDGYSVT 420
           +VF                         +E+KG+VCVTGGTGFIGSW++K+LL+DGYSV 
Sbjct: 301 FVFKYGLEEMLDDAIQCCKEKGLPVSQMEESKGRVCVTGGTGFIGSWIIKKLLQDGYSVN 360

Query: 421 TTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFHVATPIDLED 480
           TTVR DPE KKD SFLT+LPGAS++LQI+ ADL++P SF+ AIEGCIGVFHVATPID E 
Sbjct: 361 TTVRTDPEHKKDVSFLTSLPGASQRLQIFSADLSNPESFSAAIEGCIGVFHVATPIDFEL 420

Query: 481 KEPAEAVIRRTIDGTLGILKACLNSKTVRRVVYTSSVATIQFSYNKVDILDESCWSDIDY 540
           KEP E V +RTI+GTL ILKACLNSKTV+RVVYTSS + + F+  KV+++DES WSD+D 
Sbjct: 421 KEPEEVVTKRTIEGTLSILKACLNSKTVKRVVYTSSASAVNFN-GKVEMMDESYWSDVDC 480

Query: 541 INNIAPFGRSYPISKTLTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKLPSSVRVTMAL 588
           +    PFG SY +SK LTE+AVL+F +++GL+VVT+I P+V GPFICPKLPSSV  T+  
Sbjct: 481 LRASKPFGWSYAVSKALTEKAVLEFGEQNGLDVVTLIPPFVFGPFICPKLPSSVDATLNF 540

BLAST of Sgr025714 vs. NCBI nr
Match: KAG7013375.1 (hypothetical protein SDJN02_23539, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 624.4 bits (1609), Expect = 1.0e-174
Identity = 344/638 (53.92%), Postives = 394/638 (61.76%), Query Frame = 0

Query: 61  MEGGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPGASEKL 120
           ME GAKGKV VTGGTGFI SWL+K LLE GYSVTTTVR+DPE +KDY FLTNLPGASEKL
Sbjct: 1   MENGAKGKVCVTGGTGFIASWLIKSLLEKGYSVTTTVRADPEMRKDYGFLTNLPGASEKL 60

Query: 121 QIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNSK 180
           QIY ADLD+ NSFAPAI GCIGVFH+ATP+D  DKE VEV  RR+I+GTLGILK CL+SK
Sbjct: 61  QIYHADLDEPNSFAPAIAGCIGVFHLATPIDASDKEGVEVLTRRTIEGTLGILKVCLDSK 120

Query: 181 --------------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 240
                                      CWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS
Sbjct: 121 TVRRVVYTSAAATMQFNHHTVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFS 180

Query: 241 QEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILG----------------------- 300
           Q++GLEVVT+LPTYV+GPFICPRLP S+  T +L+ G                       
Sbjct: 181 QQYGLEVVTVLPTYVVGPFICPRLPDSIRVTTSLMSGNKADYGLILKSNMVHVDDVARAH 240

Query: 301 -------------------------ISLRFARWVFDEAKGKVCVTGGTGFIGSWL-VKRL 360
                                    ++L  A++   +      +    G++ S +  K+L
Sbjct: 241 IFLFEHPNANGRYVCSSHIITLEELVNLFSAKYPEFQIPSSESLKDVKGYLFSDVSSKKL 300

Query: 361 LEDGYSVTTTVR--FD----------------------------------PEKKKDYSFL 420
           L+ G+     +   FD                                   EK KDY FL
Sbjct: 301 LDAGFQYKYGIEEMFDGAIQSCKEKGYLHETNKTIVRNGRSSKFGGFKALEEKNKDYGFL 360

Query: 421 TNLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTL 480
           T+LP ASEKLQIY ADLNDPNSFAPAI GCIGVFH+ATPID +D+E  E + RR I+GTL
Sbjct: 361 TSLPNASEKLQIYHADLNDPNSFAPAIAGCIGVFHLATPIDEKDEEDVEVLTRRAIEGTL 420

Query: 481 GILKACLNSKTVRRVVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKT 540
           GILK CL+SKTVRRVV+ SSV T+ F+++ VD+LDESCWSDIDYIN+ +PFG        
Sbjct: 421 GILKICLDSKTVRRVVHASSVVTMDFNHHTVDVLDESCWSDIDYINSASPFG-------- 480

Query: 541 LTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNM 588
                                                             EYG +LKSNM
Sbjct: 481 --------------------------------------------------EYGLLLKSNM 540

BLAST of Sgr025714 vs. NCBI nr
Match: XP_021279705.1 (vestitone reductase-like [Herrania umbratica])

HSP 1 Score: 621.3 bits (1601), Expect = 8.4e-174
Identity = 302/564 (53.55%), Postives = 399/564 (70.74%), Query Frame = 0

Query: 52  RAGKEREREME-GGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFL 111
           R  ++R R+ + GG KG V VTGGTG+IGSWL+K LLE GY+V TT+R DP  K+D SFL
Sbjct: 17  RVRRKRYRDYKMGGDKGTVCVTGGTGYIGSWLIKVLLEQGYAVHTTIRPDPGNKRDVSFL 76

Query: 112 TNLPGASEKLQIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTL 171
           T LPGA E+L+I+RADL D  +F  AIEGC GV HVA+P+D +D EP  V  +R+IDG L
Sbjct: 77  TTLPGADERLKIFRADLSDPETFGAAIEGCKGVLHVASPMDFQDNEPEAVVTKRAIDGAL 136

Query: 172 GILKACLNSK--------------------------HCWSDIDYINNIAPFGRSYPISKT 231
           GILK CL SK                            W+D+DY+  +  +  SY I+KT
Sbjct: 137 GILKTCLRSKTVKRVVFTSSIASVYFNKKDVDMMDESFWTDVDYVRELKSYVSSYAITKT 196

Query: 232 LTEQAVLEFSQEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILGISLRFA-RWVFDE 291
           LTE+A LEF+ EHG++++T++P  V+GPFICP+L  SV   +A IL  S     R     
Sbjct: 197 LTEKAALEFAAEHGMDLLTVIPPLVVGPFICPKLTGSVRAALAPILESSKEERYRDQMGG 256

Query: 292 AKGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYR 351
            KG VCVTGGTGF+GSWLVK LLE GY+V TT+R DPE K+D SFLT+LPGA E+L I+R
Sbjct: 257 DKGAVCVTGGTGFVGSWLVKVLLEQGYTVHTTIRPDPENKRDVSFLTSLPGADERLNIFR 316

Query: 352 ADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRR 411
           ADL+ P SF  AIEGC GV HVA+P+D ++ EP   V +R  DG LGILK CL SKTV+R
Sbjct: 317 ADLSCPESFGAAIEGCKGVLHVASPMDFQNNEPEAVVTKRAFDGALGILKTCLRSKTVKR 376

Query: 412 VVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHG 471
           VV+TSS+A++ F+   VD++DES W+D+DY+  +  +  SY I+KT+TE+AVL+F+ EHG
Sbjct: 377 VVFTSSIASVYFNNKNVDMMDESFWTDVDYVRELQWYVSSYAITKTMTEKAVLEFAAEHG 436

Query: 472 LEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAHIFLF 531
           +++VTV+ P VVGPFICPKL  SVR  +A +LGN+ +Y  +L   MVH+DD+ARA IFL 
Sbjct: 437 MDLVTVVPPLVVGPFICPKLTGSVRAALAPILGNKDDYSLLLNVAMVHIDDLARAFIFLL 496

Query: 532 EHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYISTNVSSKKLLDAG 588
           EHP A GRY C S T+T +++ + LSA YPE+ +P +E L++++G     +SSKKLLD G
Sbjct: 497 EHPEAKGRYNCCSDTVTAQKIVEILSANYPEYTLPIVECLEEIEGPKMPGLSSKKLLDLG 556

BLAST of Sgr025714 vs. NCBI nr
Match: CBI38845.3 (unnamed protein product, partial [Vitis vinifera])

HSP 1 Score: 610.5 bits (1573), Expect = 1.5e-170
Identity = 314/663 (47.36%), Postives = 414/663 (62.44%), Query Frame = 0

Query: 64  GAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPGASEKLQIY 123
           G KGKV VTGGTGFI SWL+ +LL+ GYSV  T+RS P+ KKD  +LTNLPGASE+LQI+
Sbjct: 3   GEKGKVCVTGGTGFIASWLIMKLLQHGYSVNATIRSHPQIKKDIRYLTNLPGASERLQIF 62

Query: 124 RADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNSK--- 183
           +ADL +  SF  AIEGCIGVFHVA PVD E+ EP E   RRS++GTLGILK CL SK   
Sbjct: 63  KADLSEPQSFDAAIEGCIGVFHVAHPVDFEEGEPQETVIRRSVEGTLGILKGCLKSKTVK 122

Query: 184 -----------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFSQEH 243
                                    WSDID IN+I P G SY ISKT  E+A LEF+++H
Sbjct: 123 RVVYTSSTAAVVYNNKDEDIMDESSWSDIDVINSIKPLGSSYVISKTRIERAALEFAEQH 182

Query: 244 GLEVVTILPTYVIGPFICPRLPSSVCTTMALILG-------------------------- 303
           GL++V+++P++V+GPFICP  P SV   +A+ILG                          
Sbjct: 183 GLDLVSLIPSFVVGPFICPGFPGSVHLILAMILGNQHHYQYLKNTSMVHVDDVASAHIFL 242

Query: 304 -------------------------ISLRFARW--------------------------- 363
                                    +S ++ +                            
Sbjct: 243 LEYPDAKGRYICSSDILTLNEMSELLSAKYPQLPIPTIDSLKEIQGFRIPGASSKKLLDA 302

Query: 364 ----------VFDEA-------------------------KGKVCVTGGTGFIGSWLVKR 423
                     +F+EA                         KG+VCVTGGTGFI SWL+ +
Sbjct: 303 GFKYKYGVDEMFEEAIQCCKEKGFLNFSPDKQREMEGENEKGRVCVTGGTGFIASWLIMK 362

Query: 424 LLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFH 483
           LL+ GYSV  T+R  P  KKD S++TNLPGASEKLQI+ ADL++P+SF PA+EGCIGVFH
Sbjct: 363 LLQHGYSVNATIRSHPGSKKDISYITNLPGASEKLQIFNADLSEPHSFEPALEGCIGVFH 422

Query: 484 VATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRVVYTSSVATIQFSYNKVDILD 543
           VA P+D E +EP E V RR+++GTL ILKACLNSKTV+RVVYTSS + + F+    D+ D
Sbjct: 423 VAHPVDFEAREPEETVTRRSVEGTLAILKACLNSKTVKRVVYTSSASAVVFNDKDEDMKD 482

Query: 544 ESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKLP 588
           ES WSD+++I ++  F   Y ISKT TE+A L+F+++HGL++VT++  +VVGPF+CP LP
Sbjct: 483 ESSWSDVEFIRSLGSFAGPYMISKTETERAALEFAEKHGLDLVTLLPSFVVGPFLCPFLP 542

BLAST of Sgr025714 vs. ExPASy Swiss-Prot
Match: Q40316 (Vestitone reductase OS=Medicago sativa OX=3879 PE=1 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 2.4e-115
Identity = 188/325 (57.85%), Postives = 259/325 (79.69%), Query Frame = 0

Query: 263 EAKGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIY 322
           E KG+VCVTGGTGF+GSW++K LLE+GYSV TT+R DPE+K+D SFLTNLPGASEKL  +
Sbjct: 3   EGKGRVCVTGGTGFLGSWIIKSLLENGYSVNTTIRADPERKRDVSFLTNLPGASEKLHFF 62

Query: 323 RADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVR 382
            ADL++P+SFA AIEGC+G+FH A+PID    EP E V +RT+DG LGILKAC+NSKTV+
Sbjct: 63  NADLSNPDSFAAAIEGCVGIFHTASPIDFAVSEPEEIVTKRTVDGALGILKACVNSKTVK 122

Query: 383 RVVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEH 442
           R +YTSS + + F+    D+LDES WSD+D + ++ PFG +Y +SKTL E+AVL+F +++
Sbjct: 123 RFIYTSSGSAVSFNGKDKDVLDESDWSDVDLLRSVKPFGWNYAVSKTLAEKAVLEFGEQN 182

Query: 443 GLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAHIFL 502
           G++VVT+ILP++VG F+CPKLP S+   + L+LG + + G + + +MVHVDDVARAHI+L
Sbjct: 183 GIDVVTLILPFIVGRFVCPKLPDSIEKALVLVLGKKEQIG-VTRFHMVHVDDVARAHIYL 242

Query: 503 FEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYISTNVSSKKLLDA 562
            E+    GRY CS   + +EE+++ LSAKYPE+QI +++ LK++KG    ++++KKL+DA
Sbjct: 243 LENSVPGGRYNCSPFIVPIEEMSQLLSAKYPEYQILTVDELKEIKGARLPDLNTKKLVDA 302

Query: 563 GFEYKYGIDEMFDGAIQSCKEKGYL 588
           GF++KY I++MFD AIQ CKEKGYL
Sbjct: 303 GFDFKYTIEDMFDDAIQCCKEKGYL 326

BLAST of Sgr025714 vs. ExPASy Swiss-Prot
Match: O22133 (Protein BRI1-5 ENHANCED 1 OS=Arabidopsis thaliana OX=3702 GN=BEN1 PE=2 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 1.0e-89
Identity = 172/328 (52.44%), Postives = 225/328 (68.60%), Query Frame = 0

Query: 266 GKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPE-KKKDYSFLTNLPGASEKLQIYRA 325
           G VCVTGG+GF+ SWL+ RLL+ GYSV  TVR + E  KKD S+LT LP ASE+LQI+ A
Sbjct: 38  GLVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTA 97

Query: 326 DLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRV 385
           DLN+P SF PAIEGC  VFHVA P+D    E  E V +RT+ G +GILK+CL++KTV+R 
Sbjct: 98  DLNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRF 157

Query: 386 VYTSSVATIQFSYNK---VDILDESCWSDIDYINNIAP--FGRSYPISKTLTEQAVLKFS 445
            YTSS  T+ +S         +DES WSD++   N        SY +SK   E A L+F 
Sbjct: 158 FYTSSAVTVFYSGGNGGGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFG 217

Query: 446 QEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAH 505
            ++GLEVVT+++P VVGPFI   LPSSV +++A++ GN  E       NMVH+DDVARA 
Sbjct: 218 GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKYLFDTYNMVHIDDVARAM 277

Query: 506 IFLFEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYISTNVSSKKL 565
           IFL E P A GRYICSS  + ++E+ +FLS K+P+FQ+PS++ L   K      +SSKKL
Sbjct: 278 IFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSID-LNKYKVEKRMGLSSKKL 337

Query: 566 LDAGFEYKYGIDEMFDGAIQSCKEKGYL 588
             AGFE+KYG +E+F GAI+SC+ +G+L
Sbjct: 338 KSAGFEFKYGAEEIFSGAIRSCQARGFL 364

BLAST of Sgr025714 vs. ExPASy Swiss-Prot
Match: Q9XES5 (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Malus domestica OX=3750 GN=DFR PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 2.0e-85
Identity = 153/321 (47.66%), Postives = 216/321 (67.29%), Query Frame = 0

Query: 268 VCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLN 327
           VCVTG +GFIGSWLV RLLE GY+V  TVR DP  +K    L +LP A   L +++ADL 
Sbjct: 8   VCVTGASGFIGSWLVMRLLEHGYTVRATVR-DPTNQKKVKHLLDLPKAETHLTLWKADLA 67

Query: 328 DPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRVVYT 387
           D  SF  AI+GC GVFHVATP+D E K+P   VI+ TI+G L ILKAC  +KTVR++V+T
Sbjct: 68  DEGSFDEAIQGCSGVFHVATPMDFESKDPENEVIKPTINGLLDILKACQKAKTVRKLVFT 127

Query: 388 SSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHGLEVV 447
           SS  T+    ++  + DES WSD+++  ++   G  Y +SKTL EQA  K+++E+ ++ +
Sbjct: 128 SSAGTVNVEEHQKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFI 187

Query: 448 TVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAHIFLFEHPN 507
           T+I   V+GPF+ P +P S+   ++ +L NE+ YG I +   VH+DD+  +HI+L+EHP 
Sbjct: 188 TIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYEHPK 247

Query: 508 ANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYIS-TNVSSKKLLDAGFEY 567
           A GRYICSSH  T+ EL K L  KYPE+ IP+    K +   +   + SSKKL + GFE+
Sbjct: 248 AEGRYICSSHDATIHELVKMLREKYPEYNIPT--KFKGIDDNLEPVHFSSKKLREIGFEF 307

Query: 568 KYGIDEMFDGAIQSCKEKGYL 588
           KY +++MF GA+ +C+ KG +
Sbjct: 308 KYSLEDMFVGAVDACRAKGLI 325

BLAST of Sgr025714 vs. ExPASy Swiss-Prot
Match: Q84KP0 (Bifunctional dihydroflavonol 4-reductase/flavanone 4-reductase OS=Pyrus communis OX=23211 GN=DFR PE=1 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.3e-84
Identity = 151/321 (47.04%), Postives = 216/321 (67.29%), Query Frame = 0

Query: 268 VCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLN 327
           VCVTG +GFIGSWLV RLLE GY+V  TVR DP  +K    L +LP A   L +++ADL 
Sbjct: 8   VCVTGASGFIGSWLVMRLLEHGYTVRATVR-DPTNQKKVKHLLDLPKAETHLTLWKADLA 67

Query: 328 DPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRVVYT 387
           D  SF  AI+GC GVFHVATP+D E ++P   VI+ TI+G L ILKAC  +KTVR++V+T
Sbjct: 68  DEGSFDEAIQGCSGVFHVATPMDFESRDPENEVIKPTINGLLDILKACQKAKTVRKLVFT 127

Query: 388 SSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHGLEVV 447
           SS  T+    ++  + DES WSD+++  ++   G  Y +SKTL EQA  K+++E+ ++ +
Sbjct: 128 SSAGTVNVEEHQKPVYDESNWSDVEFCRSVKMTGWMYFVSKTLAEQAAWKYAKENNIDFI 187

Query: 448 TVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAHIFLFEHPN 507
           T+I   V+GPF+ P +P S+   ++ +L NE+ YG I +   VH+DD+  +HI+L++HP 
Sbjct: 188 TIIPTLVIGPFLMPSMPPSLITGLSPILRNESHYGIIKQGQYVHLDDLCLSHIYLYKHPK 247

Query: 508 ANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYIS-TNVSSKKLLDAGFEY 567
           A GRYICSSH  T+ EL K L  KYPE+ IP+    K +   +   + SSKKL + GFE+
Sbjct: 248 AEGRYICSSHDATIHELVKMLREKYPEYNIPT--KFKGIDDNLEPVHFSSKKLREIGFEF 307

Query: 568 KYGIDEMFDGAIQSCKEKGYL 588
           KY +++MF GA+ +C+ KG +
Sbjct: 308 KYSLEDMFVGAVDACRAKGLI 325

BLAST of Sgr025714 vs. ExPASy Swiss-Prot
Match: I3PLR3 (Noscapine synthase SDR1 OS=Papaver somniferum OX=3469 GN=SDR1 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.1e-83
Identity = 164/343 (47.81%), Postives = 228/343 (66.47%), Query Frame = 0

Query: 252 ISLRFARWVFDEAKGK-VCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLT 311
           IS R+ ++   E  GK VCVTGG G++ SWL+ RLLE GYSV TTVR DP+ ++D S L 
Sbjct: 7   ISERYQKFKEMEGTGKIVCVTGGAGYLASWLIMRLLERGYSVRTTVRSDPKFREDVSHLK 66

Query: 312 NLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLG 371
            LP A+EKLQI+ ADL +P SF  AI GC+GVF VA  ++  ++   E +I+  ++GTL 
Sbjct: 67  ALPEATEKLQIFEADLENPESFDDAINGCVGVFLVAQGMNFAEEYTLEKIIKTCVEGTLR 126

Query: 372 ILKACLNSKTVRRVVYTSS--VATIQFSYNKVDILDESCWSDIDYI----NNIAPFGRSY 431
           IL++CL SKTV++VVYTSS   A +  +   V  +DE+ WS++D        + P   SY
Sbjct: 127 ILQSCLKSKTVKKVVYTSSADAAMMISNLKAVKEIDETIWSEVDNFISKPEQVIPGLPSY 186

Query: 432 PISKTLTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCI 491
            +SK LTE+A LKFS+EHGL+VVT++ P VVGPFI P  P SV + ++++ G+ +    +
Sbjct: 187 VVSKVLTERACLKFSEEHGLDVVTILPPLVVGPFITPHPPPSVSIALSIISGDVSMMLGV 246

Query: 492 LKSNMVHVDDVARAHIFLFEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLK 551
              N VH+DDVA AHIF+FE   A GR+ICSS    + +L KF+S  YPEF +P+ + LK
Sbjct: 247 RLENAVHIDDVALAHIFVFECEKAKGRHICSSVDFPMHDLPKFISENYPEFNVPT-DLLK 306

Query: 552 DVKGYISTNVSSKKLLDAGFEYKYGIDEMFDGAIQSCKEKGYL 588
           D++     ++SS KLL  GF++KY   E+F  AI+  KEKG+L
Sbjct: 307 DIEEQEPVHLSSDKLLSMGFQFKYDFAEIFGDAIRCAKEKGFL 348

BLAST of Sgr025714 vs. ExPASy TrEMBL
Match: A0A6J0ZYD6 (vestitone reductase-like OS=Herrania umbratica OX=108875 GN=LOC110413263 PE=4 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 4.1e-174
Identity = 302/564 (53.55%), Postives = 399/564 (70.74%), Query Frame = 0

Query: 52  RAGKEREREME-GGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFL 111
           R  ++R R+ + GG KG V VTGGTG+IGSWL+K LLE GY+V TT+R DP  K+D SFL
Sbjct: 17  RVRRKRYRDYKMGGDKGTVCVTGGTGYIGSWLIKVLLEQGYAVHTTIRPDPGNKRDVSFL 76

Query: 112 TNLPGASEKLQIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTL 171
           T LPGA E+L+I+RADL D  +F  AIEGC GV HVA+P+D +D EP  V  +R+IDG L
Sbjct: 77  TTLPGADERLKIFRADLSDPETFGAAIEGCKGVLHVASPMDFQDNEPEAVVTKRAIDGAL 136

Query: 172 GILKACLNSK--------------------------HCWSDIDYINNIAPFGRSYPISKT 231
           GILK CL SK                            W+D+DY+  +  +  SY I+KT
Sbjct: 137 GILKTCLRSKTVKRVVFTSSIASVYFNKKDVDMMDESFWTDVDYVRELKSYVSSYAITKT 196

Query: 232 LTEQAVLEFSQEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILGISLRFA-RWVFDE 291
           LTE+A LEF+ EHG++++T++P  V+GPFICP+L  SV   +A IL  S     R     
Sbjct: 197 LTEKAALEFAAEHGMDLLTVIPPLVVGPFICPKLTGSVRAALAPILESSKEERYRDQMGG 256

Query: 292 AKGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYR 351
            KG VCVTGGTGF+GSWLVK LLE GY+V TT+R DPE K+D SFLT+LPGA E+L I+R
Sbjct: 257 DKGAVCVTGGTGFVGSWLVKVLLEQGYTVHTTIRPDPENKRDVSFLTSLPGADERLNIFR 316

Query: 352 ADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRR 411
           ADL+ P SF  AIEGC GV HVA+P+D ++ EP   V +R  DG LGILK CL SKTV+R
Sbjct: 317 ADLSCPESFGAAIEGCKGVLHVASPMDFQNNEPEAVVTKRAFDGALGILKTCLRSKTVKR 376

Query: 412 VVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHG 471
           VV+TSS+A++ F+   VD++DES W+D+DY+  +  +  SY I+KT+TE+AVL+F+ EHG
Sbjct: 377 VVFTSSIASVYFNNKNVDMMDESFWTDVDYVRELQWYVSSYAITKTMTEKAVLEFAAEHG 436

Query: 472 LEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAHIFLF 531
           +++VTV+ P VVGPFICPKL  SVR  +A +LGN+ +Y  +L   MVH+DD+ARA IFL 
Sbjct: 437 MDLVTVVPPLVVGPFICPKLTGSVRAALAPILGNKDDYSLLLNVAMVHIDDLARAFIFLL 496

Query: 532 EHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYISTNVSSKKLLDAG 588
           EHP A GRY C S T+T +++ + LSA YPE+ +P +E L++++G     +SSKKLLD G
Sbjct: 497 EHPEAKGRYNCCSDTVTAQKIVEILSANYPEYTLPIVECLEEIEGPKMPGLSSKKLLDLG 556

BLAST of Sgr025714 vs. ExPASy TrEMBL
Match: A0A371E4K7 (Uncharacterized protein OS=Mucuna pruriens OX=157652 GN=CR513_60885 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 9.4e-171
Identity = 310/645 (48.06%), Postives = 407/645 (63.10%), Query Frame = 0

Query: 66  KGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPGASEKLQIYRA 125
           KG+V VTGGTGF+GSW++KRLLEDGY+V TT+RSDPE K+D  FLTNL GASEKL+I+ A
Sbjct: 5   KGRVCVTGGTGFLGSWIIKRLLEDGYAVNTTIRSDPEGKRDVRFLTNLAGASEKLKIFNA 64

Query: 126 DLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNSK----- 185
           DL +  SF PA+EGC+G+FH ATP+D    E  EV  +R+IDG LGIL+AC+NSK     
Sbjct: 65  DLSEPESFGPAVEGCVGIFHTATPIDFALNEAEEVVTKRTIDGALGILRACVNSKTVKRV 124

Query: 186 ----------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEFSQEHG 245
                                   WSD+D++  + PFG SY +SK L E+A L+F  ++G
Sbjct: 125 VYTSSASTVSFSGAEVNDQVDESVWSDVDFLRRVKPFGWSYAVSKVLAEKAALQFGAQNG 184

Query: 246 LEVVTILPTYVIGPFICPRLPSSVCTTMALILG--------------------------- 305
           L+VVT++P +V+G F+CP+LP SV   + L+ G                           
Sbjct: 185 LDVVTLIPPFVVGRFVCPKLPDSVHRALLLLFGKKEEIGVIRYHMVHVDDVARAHIFLLE 244

Query: 306 -----------------------ISLRFARW----------------------------- 365
                                  +S ++  +                             
Sbjct: 245 HPNPNGRYNCSPFIVPIEEISRLLSAKYPEYQIPSVDEVKEIKGAKLPHLISKKLVDAGF 304

Query: 366 --------VFDEA---------KGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEK 425
                   +F++A         KG+VCVTGGTGFIGSW++KRLLEDGY+V TT+R DP+ 
Sbjct: 305 EFKYSVEDMFEDAIECCKEKEGKGRVCVTGGTGFIGSWIIKRLLEDGYTVNTTIRSDPDG 364

Query: 426 KKDYSFLTNLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIR 485
           K+D SFL NLPGAS KL+I+ ADL+ P SF+ AIEGC+GV H ATPIDLE KE  E V +
Sbjct: 365 KRDVSFLRNLPGASRKLKIFNADLSKPESFSEAIEGCVGVLHTATPIDLEVKEAEETVTK 424

Query: 486 RTIDGTLGILKACLNSKTVRRVVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGR 545
           R+IDG LGILKACLNSKTV+RVVYTSS + + +   + D++DES WSD + + ++ PF  
Sbjct: 425 RSIDGALGILKACLNSKTVKRVVYTSSASAVYWQAKEEDVMDESYWSDDNLLRSLKPFAW 484

Query: 546 SYPISKTLTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYG 588
           SY ISKTL E+AVL F Q HGL+VVT+I  +VVGPFICPKLP SV  ++A + G +  +G
Sbjct: 485 SYSISKTLAERAVLDFGQLHGLDVVTLIPTFVVGPFICPKLPGSVYTSLAFLFGQKNPFG 544

BLAST of Sgr025714 vs. ExPASy TrEMBL
Match: A0A5N5NP35 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_002875 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 2.7e-170
Identity = 325/692 (46.97%), Postives = 428/692 (61.85%), Query Frame = 0

Query: 56  EREREMEGGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPG 115
           EREREME G +G+V VTGGTG++ SWL+ +LLE GY V TTVR  PE K+D SFLT+LPG
Sbjct: 36  EREREME-GERGRVCVTGGTGYLASWLIMKLLEQGYLVNTTVRPHPEHKRDVSFLTSLPG 95

Query: 116 ASEKLQIYRADLDDQNSFAPAIEGCIGVFHVATPV--DLEDKEPVEVARRRSIDGTLGIL 175
            SE+LQI+ ADL D + FA A+ GCIGVFHVATP+  D  + EP EV   R+I+GTLGIL
Sbjct: 96  GSERLQIFYADLSDPSGFAAALTGCIGVFHVATPLPHDFGNGEPEEVVIERAINGTLGIL 155

Query: 176 KACLNSK--------------------------HCWSDIDYINNIAPFGRSYPISKTLTE 235
           KACLNSK                            WS++DY+       R Y ISKTLTE
Sbjct: 156 KACLNSKTVKRVVYTSSASAVVFNDNGEEMMDESYWSNVDYLKASNLSLRPYFISKTLTE 215

Query: 236 QAVLEFSQEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILG---------------- 295
           +  LEF++EHGL++VT++P+YV+GPFICP +P+SV  ++A++LG                
Sbjct: 216 KRALEFAEEHGLDLVTLIPSYVLGPFICPNMPASVLASLAMVLGNREEYGSLIETSMVHI 275

Query: 296 -----------------------------------ISLRFARW----------------- 355
                                              +S +F  +                 
Sbjct: 276 DDVARAHIFLLQNPEAKGRYICSSHMITIEEMSKFLSAKFPEYPIPTLEYLKDVKGSKSS 335

Query: 356 ------------------------------------------------------------ 415
                                                                       
Sbjct: 336 DVSSKKLLDSGFKFSLLIVPTVKGSKSSYVSSKKLLDSGFKFRHGRRLATTILSELILER 395

Query: 416 --VFDEAKGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASE 475
               +  +G+VCVTGGTG++ SWL+ +LLE GY V TTVR  PE K+D SFLT+LPG SE
Sbjct: 396 EREMEGERGRVCVTGGTGYVASWLIMKLLEQGYLVNTTVRPHPEHKRDVSFLTSLPGGSE 455

Query: 476 KLQIYRADLNDPNSFAPAIEGCIGVFHVATPIDLE--DKEPAEAVIRRTIDGTLGILKAC 535
           +LQI+ ADL++PN FA AI+GCIGVFHVATPI L+    EP E VI+R ++GTLGIL+AC
Sbjct: 456 RLQIFYADLSEPNGFAAAIKGCIGVFHVATPIPLDFGKGEPDEVVIQRALNGTLGILRAC 515

Query: 536 LNSKTVRRVVYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAV 588
           LNSKTV+RVVYTSS A + F+ +  +I+DES WS++DY+       R Y ISKTLTE+  
Sbjct: 516 LNSKTVKRVVYTSSAAAVVFNDSGEEIMDESYWSNVDYLKASNLSLRPYFISKTLTEKRA 575

BLAST of Sgr025714 vs. ExPASy TrEMBL
Match: A0A6N2MJF0 (Uncharacterized protein OS=Salix viminalis OX=40686 GN=SVIM_LOCUS378741 PE=3 SV=1)

HSP 1 Score: 598.2 bits (1541), Expect = 3.7e-167
Identity = 310/664 (46.69%), Postives = 420/664 (63.25%), Query Frame = 0

Query: 61  MEGGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPGASEKL 120
           ME G KG V VTGGTGF+ SWL+ RLLE GY+V TT+RS P   KD S+LTNLP A+EKL
Sbjct: 167 MEEG-KGLVCVTGGTGFVASWLIMRLLEHGYTVRTTIRSSPGTSKDISYLTNLPRAAEKL 226

Query: 121 QIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNSK 180
           Q++ ADLDD +SF  AI GC+GVFH+A P+D  ++EP EV  +R++DGTLG+L+AC+N+K
Sbjct: 227 QVFNADLDDPDSFNEAIGGCMGVFHLAFPLDFAEREPEEVITKRAVDGTLGVLRACVNAK 286

Query: 181 ---------------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEF 240
                                        W+ IDY  ++  FG SY ++K  TE+A L+F
Sbjct: 287 TVKRVVCASSQATVIYGGDGDEKVADESSWTSIDYYRSLNRFGTSYLVAKNKTERAALDF 346

Query: 241 SQEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILG---------------------- 300
           ++++GL++V ++P  ++GPFICPR+P S+   ++LI G                      
Sbjct: 347 AEQYGLDLVFLIPPLIVGPFICPRIPESIRWALSLIFGEKRLYHLLVKLNVVHTDDVAMA 406

Query: 301 --------------------ISL------------------------------------- 360
                               IS+                                     
Sbjct: 407 YIFLLESPHAKGRYICSWEEISINEMSDFLSARYPEFQTPTKDSLKDIKGFKMRSLSPKK 466

Query: 361 ------------------RFARWVFDE-----------AKGKVCVTGGTGFIGSWLVKRL 420
                             R A  +  E            +G+VCVTGGTG++ SWL+ +L
Sbjct: 467 LLDCGFKFEHGLVDIHGRRLATTILSELILEREREMEGERGRVCVTGGTGYLASWLIMKL 526

Query: 421 LEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFHV 480
           LE GY V TTVR  PE K+D SFLT+LPG SE+LQI+ ADL+DP  FA AI+GCIGVFHV
Sbjct: 527 LEQGYLVNTTVRPHPEHKRDVSFLTSLPGGSERLQIFYADLSDPTGFAAAIKGCIGVFHV 586

Query: 481 ATPIDLE--DKEPAEAVIRRTIDGTLGILKACLNSKTVRRVVYTSSVATIQFSYNKVDIL 540
           ATP+ L+    EP E VI R I+GTLGIL+ACLNSKTV+RVVYTSS + + F+ +  +++
Sbjct: 587 ATPLPLDFGKGEPEEVVIERAINGTLGILRACLNSKTVKRVVYTSSASAVVFNDSGEEMM 646

Query: 541 DESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKL 588
           DES WS++DY+       R Y ISKTLTE+  L+F++EHGL++VT+I  Y++GPFICP +
Sbjct: 647 DESYWSNVDYLKASNLSLRPYFISKTLTEKRALEFAEEHGLDLVTLIPTYILGPFICPNM 706

BLAST of Sgr025714 vs. ExPASy TrEMBL
Match: A0A5N5NPX3 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_002890 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 8.3e-167
Identity = 311/664 (46.84%), Postives = 420/664 (63.25%), Query Frame = 0

Query: 61  MEGGAKGKVRVTGGTGFIGSWLVKRLLEDGYSVTTTVRSDPEKKKDYSFLTNLPGASEKL 120
           ME G KG V VTGGTGF+ SWL+ RLLE GY+V TT+RS P   KD S+LTNLP A+EKL
Sbjct: 1   MEEG-KGLVCVTGGTGFVASWLIMRLLEHGYTVRTTIRSSPGISKDISYLTNLPRAAEKL 60

Query: 121 QIYRADLDDQNSFAPAIEGCIGVFHVATPVDLEDKEPVEVARRRSIDGTLGILKACLNSK 180
           Q++ ADLDD +SF  AI GC+GVFH+A P+D  ++EP EV  +R++DGTLG+L+AC+N+K
Sbjct: 61  QVFNADLDDPDSFNEAIGGCMGVFHLAFPLDFAEREPEEVITKRAVDGTLGVLRACVNAK 120

Query: 181 ---------------------------HCWSDIDYINNIAPFGRSYPISKTLTEQAVLEF 240
                                        W++IDY  ++  FG SY ++K  TE+A L+F
Sbjct: 121 TVKRVVCASSQATVIYGGDGDEKVVDESSWTNIDYYRSLNRFGTSYLVAKNKTERAALDF 180

Query: 241 SQEHGLEVVTILPTYVIGPFICPRLPSSVCTTMALILG---------------------- 300
           ++++GL++V ++P  ++GPFIC R+P S+   ++LI G                      
Sbjct: 181 AEQYGLDLVFLIPPLIVGPFICHRIPESIRWALSLIFGEKRLYHLLVKLNVVHTDDVARA 240

Query: 301 --------------------ISL------------------------------------- 360
                               IS+                                     
Sbjct: 241 YIFLLEFPHAKGRYICSREEISINEMSEFLSARYPEFQTPTKDSLKDIKGFKMRSLSPKK 300

Query: 361 ------------------RFARWVFDE-----------AKGKVCVTGGTGFIGSWLVKRL 420
                             R A  +  E            +G+VCVTGGTG++ SWL+ +L
Sbjct: 301 LLDCGFKFEHGLVDIHGRRLATTILSELILEREREMEGERGRVCVTGGTGYLASWLIMKL 360

Query: 421 LEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLNDPNSFAPAIEGCIGVFHV 480
           LE GY V TTVR  PE K+D SFLT+LPG SE+LQI+ ADL+DP+ FA A++GCIGVFHV
Sbjct: 361 LEQGYLVNTTVRPHPEHKRDVSFLTSLPGGSERLQIFYADLSDPSGFAAALKGCIGVFHV 420

Query: 481 ATPI--DLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRVVYTSSVATIQFSYNKVDIL 540
           ATP+  D  + EP E VI R I+GTLGILKACLNSKTV+RVVYTSS + + F+ +  +++
Sbjct: 421 ATPLPHDFGNGEPEEVVIERAINGTLGILKACLNSKTVKRVVYTSSASAVVFNDSGEEMM 480

Query: 541 DESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHGLEVVTVILPYVVGPFICPKL 588
           DES WS++DY+       R Y ISKTLTE+  L+F++EHGL++VT+I  YV+GPFICP +
Sbjct: 481 DESYWSNVDYLKASNLSLRPYFISKTLTEKRALEFAEEHGLDLVTLIPSYVLGPFICPNM 540

BLAST of Sgr025714 vs. TAIR 10
Match: AT2G45400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 332.4 bits (851), Expect = 7.3e-91
Identity = 172/328 (52.44%), Postives = 225/328 (68.60%), Query Frame = 0

Query: 266 GKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPE-KKKDYSFLTNLPGASEKLQIYRA 325
           G VCVTGG+GF+ SWL+ RLL+ GYSV  TVR + E  KKD S+LT LP ASE+LQI+ A
Sbjct: 38  GLVCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTA 97

Query: 326 DLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRV 385
           DLN+P SF PAIEGC  VFHVA P+D    E  E V +RT+ G +GILK+CL++KTV+R 
Sbjct: 98  DLNEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRF 157

Query: 386 VYTSSVATIQFSYNK---VDILDESCWSDIDYINNIAP--FGRSYPISKTLTEQAVLKFS 445
            YTSS  T+ +S         +DES WSD++   N        SY +SK   E A L+F 
Sbjct: 158 FYTSSAVTVFYSGGNGGGGGEVDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFG 217

Query: 446 QEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAH 505
            ++GLEVVT+++P VVGPFI   LPSSV +++A++ GN  E       NMVH+DDVARA 
Sbjct: 218 GKNGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEKYLFDTYNMVHIDDVARAM 277

Query: 506 IFLFEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYISTNVSSKKL 565
           IFL E P A GRYICSS  + ++E+ +FLS K+P+FQ+PS++ L   K      +SSKKL
Sbjct: 278 IFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSID-LNKYKVEKRMGLSSKKL 337

Query: 566 LDAGFEYKYGIDEMFDGAIQSCKEKGYL 588
             AGFE+KYG +E+F GAI+SC+ +G+L
Sbjct: 338 KSAGFEFKYGAEEIFSGAIRSCQARGFL 364

BLAST of Sgr025714 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 307.4 bits (786), Expect = 2.5e-83
Identity = 154/328 (46.95%), Postives = 215/328 (65.55%), Query Frame = 0

Query: 265 KGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRA 324
           K  VCVTG +GFIGSWLV RLLE GY V  TVR DP   K    L +LP A   L +++A
Sbjct: 5   KETVCVTGASGFIGSWLVMRLLERGYFVRATVR-DPGNLKKVQHLLDLPNAKTLLTLWKA 64

Query: 325 DLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRV 384
           DL++  S+  AI GC GVFHVATP+D E K+P   VI+ T++G LGI+KAC+ +KTVRR 
Sbjct: 65  DLSEEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRF 124

Query: 385 VYTSSVATIQFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEHGL 444
           V+TSS  T+    ++ ++ DE+ WSD+++I +    G  Y +SKTL E+A   F++E GL
Sbjct: 125 VFTSSAGTVNVEEHQKNVYDENDWSDLEFIMSKKMTGWMYFVSKTLAEKAAWDFAEEKGL 184

Query: 445 EVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAHIFLFE 504
           + +++I   VVGPFI   +P S+   ++ +  NEA Y  I +   VH+DD+  AHIFL+E
Sbjct: 185 DFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSIIRQGQYVHLDDLCNAHIFLYE 244

Query: 505 HPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSL-----ESLKDVKGYISTNVSSKKL 564
              A GRYICSSH  T+  ++KFL  KYPE+ +PS      E+LK ++       SSKKL
Sbjct: 245 QAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDENLKSIE------FSSKKL 304

Query: 565 LDAGFEYKYGIDEMFDGAIQSCKEKGYL 588
            D GF +KY ++EMF  +I++C++KG+L
Sbjct: 305 TDMGFNFKYSLEEMFIESIETCRQKGFL 325

BLAST of Sgr025714 vs. TAIR 10
Match: AT4G35420.1 (dihydroflavonol 4-reductase-like1 )

HSP 1 Score: 265.8 bits (678), Expect = 8.4e-71
Identity = 144/329 (43.77%), Postives = 205/329 (62.31%), Query Frame = 0

Query: 262 DEAKGKVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQI 321
           D+AKGKVCVTG +GF+ SWLVKRLL +GY V  TVR DP  +K  + L  L GA E+L++
Sbjct: 2   DQAKGKVCVTGASGFLASWLVKRLLLEGYEVIGTVR-DPGNEKKLAHLWKLEGAKERLRL 61

Query: 322 YRADLNDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTV 381
            +ADL +  SF  AI GC GVFH A+P+      P E ++R  I+GTL +L++C  + ++
Sbjct: 62  VKADLMEEGSFDNAIMGCQGVFHTASPVLKPTSNPEEEILRPAIEGTLNVLRSCRKNPSL 121

Query: 382 RRVVYTSSVATIQF--SYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFS 441
           +RVV TSS +T++    ++    LDES W+ ++       F   Y +SKTL EQA  KFS
Sbjct: 122 KRVVLTSSSSTVRIRDDFDPKIPLDESIWTSVELCKR---FQVWYALSKTLAEQAAWKFS 181

Query: 442 QEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSNMVHVDDVARAH 501
           +E+G+++VTV+  ++VGP + P L S+    + L+ G   ++    +   VH+DDVAR H
Sbjct: 182 EENGIDLVTVLPSFLVGPSLPPDLCSTASDVLGLLKGETEKFQWHGQMGYVHIDDVARTH 241

Query: 502 IFLFEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPS-LESLKDVKGYISTNVSSKK 561
           I +FEH  A GRYICSS+ I+LEEL  FLSA+YP   IP   E L      +  +  + K
Sbjct: 242 IVVFEHEAAQGRYICSSNVISLEELVSFLSARYPSLPIPKRFEKL----NRLHYDFDTSK 301

Query: 562 LLDAGFEYKYGIDEMFDGAIQSCKEKGYL 588
           +   G ++K  ++EMFD  I S  E+GYL
Sbjct: 302 IQSLGLKFK-SLEEMFDDCIASLVEQGYL 321

BLAST of Sgr025714 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 245.4 bits (625), Expect = 1.2e-64
Identity = 127/332 (38.25%), Postives = 204/332 (61.45%), Query Frame = 0

Query: 267 KVCVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADL 326
           K CV GGTG + S L+K LL+ GY V TTVR DPE +K  + L  L    + L+I++ADL
Sbjct: 12  KACVIGGTGNLASILIKHLLQSGYKVNTTVR-DPENEKKIAHLRKLQELGD-LKIFKADL 71

Query: 327 NDPNSFAPAIEGCIGVFHVATPIDLEDKEPAEAVIRRTIDGTLGILKACLNSKTVRRVVY 386
            D +SF  +  GC  +FHVATPI+ + ++P + +I+  I G + +LK+CL SK+V+RV+Y
Sbjct: 72  TDEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVIY 131

Query: 387 TSSVATIQFSYNKVD----ILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLKFSQEH 446
           TSS A +  S N +     +++E  W+D++++    PF   YPISK L E+   +F++E+
Sbjct: 132 TSSAAAV--SINNLSGTGIVMNEENWTDVEFLTEEKPFNWGYPISKVLAEKTAWEFAKEN 191

Query: 447 GLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKS-------NMVHVDDV 506
            + +VTVI   + G  +    PSS+ ++M+ + G E     + +        + VHVDD+
Sbjct: 192 KINLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFVHVDDL 251

Query: 507 ARAHIFLFEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYISTNVS 566
           ARAH+FL E   A+GRYIC ++  ++ E+A FL  +YP++ +  L   ++        +S
Sbjct: 252 ARAHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNV--LSEFEEGLSIPKLTLS 311

Query: 567 SKKLLDAGFEYKYGIDEMFDGAIQSCKEKGYL 588
           S+KL++ GF ++YGI+EM+D  I+  + KG +
Sbjct: 312 SQKLINEGFRFEYGINEMYDQMIEYFESKGLI 337

BLAST of Sgr025714 vs. TAIR 10
Match: AT4G27250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 213.4 bits (542), Expect = 4.9e-55
Identity = 116/330 (35.15%), Postives = 192/330 (58.18%), Query Frame = 0

Query: 269 CVTGGTGFIGSWLVKRLLEDGYSVTTTVRFDPEKKKDYSFLTNLPGASEKLQIYRADLND 328
           CVTG +G+IGSWLVK LL+ GY+V  T+R D  K +   +  +    +E+L+++RADL D
Sbjct: 14  CVTGASGYIGSWLVKSLLQRGYTVHATLR-DLAKSE---YFQSKWKENERLRLFRADLRD 73

Query: 329 PNSFAPAIEGCIGVFHVATPIDLE--------DKEPAEAVIRRTIDGTLGILKACLNSKT 388
             SF  A++GC GVFHVA  ++ +        +      VI   + G   +L +CL SK+
Sbjct: 74  DGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVLSSCLKSKS 133

Query: 389 VRRVVYTSSVATI---QFSYNKVDILDESCWSDIDYINNIAPFGRSYPISKTLTEQAVLK 448
           V+RVV+TSS++T+     +      +DE+C + +D++      G  Y +SK ++E+   +
Sbjct: 134 VKRVVFTSSISTLTAKDENERMRSFVDETCKAHVDHVLKTQASGWIYVLSKLVSEEEAFR 193

Query: 449 FSQEHGLEVVTVILPYVVGPFICPKLPSSVRVTMALMLGNEAEYGCILKSN-------MV 508
           +++E G+++V+VI   V GPF+ P +PSSV+V ++ + G+   +  +   N       +V
Sbjct: 194 YAKERGMDLVSVITTTVSGPFLTPFVPSSVQVLLSPITGDSKLFAILSAVNKRMGSIALV 253

Query: 509 HVDDVARAHIFLFEHPNANGRYICSSHTITLEELAKFLSAKYPEFQIPSLESLKDVKGYI 568
           H++D+ RAH+FL E P A G+YIC    I + EL     +K    ++  +   ++ +  +
Sbjct: 254 HIEDICRAHLFLMEQPKAKGQYICCVDNIDMHELMLHHFSKDYLCKVQKVNEDEEERECM 313

Query: 569 STNVSSKKLLDAGFEYKYGIDEMFDGAIQS 581
              +SSKKL + GFEYKYGI+E+ D  I +
Sbjct: 314 KPIISSKKLRELGFEYKYGIEEIVDQTIDA 339