Sgr025677 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr025677
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	PMEI domain-containing protein
Location	tig00152936: 1799637 .. 1800221 (+)
RNA-Seq Expression	Sgr025677
Synteny	Sgr025677

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCCTCTCTCTCTCTCCTCTTTCTCTCTCTACATCCTTCCCTTCTTCTTCTTCTTCCTCTCCACTTTCCCCATCACAGAAGCTTCCAACACATCCTCCCTCATCTACAAAACCTGCAAAACCAGCGCAGACCAGGACCCCAACATCTCCTTCAACTTCTGCTCAACATCTCTACAGGCCGCCTCCAACCACAGCCGCCGCGGAGCCGATCTCCGCAGCCTCGGCCTCCTCTCCATCCGGCTGATCCGCCGCAACCTGACCAGCACCCGCCACCACATCAAGAAGCTACGCAGAAGAAGCAAGACCCTCGACCCGTTCGTTAAGTCGTGTTTGGCCGACTGTTTGGAGCTTTATTCCGACGCGATTCCGACCGTGAAGCAAGCCCGAAGAGATTACAGGGCCGGGCGATATTCCGACGCCGGCACCGAGATCAGTTCGGTGATGGACGACTCGTCGACCTGCGAAGATGGGTTCAAGGAAGAAGAAGGGGTGGTTTCGCCATTAACGAGCAGAAATCATAATGCCTTTGAATTGTCGGCTATTGCACTGTCCATTATTAATATGATTTGCTTGAGAAATTAG

mRNA sequence

ATGTCTCCTCTCTCTCTCTCCTCTTTCTCTCTCTACATCCTTCCCTTCTTCTTCTTCTTCCTCTCCACTTTCCCCATCACAGAAGCTTCCAACACATCCTCCCTCATCTACAAAACCTGCAAAACCAGCGCAGACCAGGACCCCAACATCTCCTTCAACTTCTGCTCAACATCTCTACAGGCCGCCTCCAACCACAGCCGCCGCGGAGCCGATCTCCGCAGCCTCGGCCTCCTCTCCATCCGGCTGATCCGCCGCAACCTGACCAGCACCCGCCACCACATCAAGAAGCTACGCAGAAGAAGCAAGACCCTCGACCCGTTCGTTAAGTCGTGTTTGGCCGACTGTTTGGAGCTTTATTCCGACGCGATTCCGACCGTGAAGCAAGCCCGAAGAGATTACAGGGCCGGGCGATATTCCGACGCCGGCACCGAGATCAGTTCGGTGATGGACGACTCGTCGACCTGCGAAGATGGGTTCAAGGAAGAAGAAGGGGTGGTTTCGCCATTAACGAGCAGAAATCATAATGCCTTTGAATTGTCGGCTATTGCACTGTCCATTATTAATATGATTTGCTTGAGAAATTAG

Coding sequence (CDS)

ATGTCTCCTCTCTCTCTCTCCTCTTTCTCTCTCTACATCCTTCCCTTCTTCTTCTTCTTCCTCTCCACTTTCCCCATCACAGAAGCTTCCAACACATCCTCCCTCATCTACAAAACCTGCAAAACCAGCGCAGACCAGGACCCCAACATCTCCTTCAACTTCTGCTCAACATCTCTACAGGCCGCCTCCAACCACAGCCGCCGCGGAGCCGATCTCCGCAGCCTCGGCCTCCTCTCCATCCGGCTGATCCGCCGCAACCTGACCAGCACCCGCCACCACATCAAGAAGCTACGCAGAAGAAGCAAGACCCTCGACCCGTTCGTTAAGTCGTGTTTGGCCGACTGTTTGGAGCTTTATTCCGACGCGATTCCGACCGTGAAGCAAGCCCGAAGAGATTACAGGGCCGGGCGATATTCCGACGCCGGCACCGAGATCAGTTCGGTGATGGACGACTCGTCGACCTGCGAAGATGGGTTCAAGGAAGAAGAAGGGGTGGTTTCGCCATTAACGAGCAGAAATCATAATGCCTTTGAATTGTCGGCTATTGCACTGTCCATTATTAATATGATTTGCTTGAGAAATTAG

Protein sequence

MSPLSLSSFSLYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSIINMICLRN

Homology

[Close]

BLAST of Sgr025677 vs. NCBI nr
Match: XP_004145025.1 (putative invertase inhibitor [Cucumis sativus])

HSP 1 Score: 245.4 bits (625), Expect = 4.2e-61
Identity = 133/193 (68.91%), Postives = 157/193 (81.35%), Query Frame = 0


Query: 6   LSSFSLYILP--FFFFFLSTFPITEAS---NTSSLIYKTCKTSADQDPNISFNFCSTSLQ 65
           +SS S  ILP  F F F S FPIT++S   NTSSLIYKTCK S++QDPNISFNFC TSL+
Sbjct: 1   MSSLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLK 60

Query: 66  -AASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSK-TLDPFVKSCLADCLEL 125
            AA+ H      LR LGL++I LIR N+++TRHHIKK   ++K   DPFVK CL DCLEL
Sbjct: 61  PAATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLEL 120

Query: 126 YSDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFE 185
           YSDAIPTVKQAR+DY+AGRY+DA  +ISSVMDD STCE+GFKE++GV+SPLT+RNHNAFE
Sbjct: 121 YSDAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFE 180

Query: 186 LSAIALSIINMIC 192
           LSAIALSIINM+C
Sbjct: 181 LSAIALSIINMLC 193

[Close]

BLAST of Sgr025677 vs. NCBI nr
Match: XP_023514422.1 (putative invertase inhibitor [Cucurbita pepo subsp. pepo])

HSP 1 Score: 244.2 bits (622), Expect = 9.3e-61
Identity = 135/191 (70.68%), Postives = 155/191 (81.15%), Query Frame = 0


Query: 6   LSSFSLYILPFFFFFLSTFPITEA--SNTSSLIYKTCKTSADQDPNISFNFCSTSLQ--- 65
           +SS SL ILP  FF  S F IT++  SNTSSLIY TCKTS+DQDPNISFNFC TSL+   
Sbjct: 1   MSSLSLSILPLLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLRHGV 60

Query: 66  -AASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELY 125
            AA+ H R   DLR LGL+S+RL+RRN++STRHHIKKL RR+K L+  VKSCLADCLELY
Sbjct: 61  VAAAKHGRSALDLRRLGLISLRLVRRNVSSTRHHIKKL-RRNKHLNTLVKSCLADCLELY 120

Query: 126 SDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFEL 185
           SDAIP +K ARRDY+  RY +   +ISSVMDD STCEDGF EEEGV+SPLT+RNHNAFEL
Sbjct: 121 SDAIPILKDARRDYKGRRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRNHNAFEL 180

Query: 186 SAIALSIINMI 191
           SAIALSIINM+
Sbjct: 181 SAIALSIINML 189

[Close]

BLAST of Sgr025677 vs. NCBI nr
Match: KAG6593721.1 (hypothetical protein SDJN03_13197, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 244.2 bits (622), Expect = 9.3e-61
Identity = 132/187 (70.59%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 6   LSSFSLYILPFFFFFLSTFPITEA--SNTSSLIYKTCKTSADQDPNISFNFCSTSLQAAS 65
           +SS S+ ILPF FF  S F IT++  SNTSSLIY TCKTS+DQDPNISFNFC TSLQ  +
Sbjct: 41  MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 100

Query: 66  NHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAI 125
            H R   DLR LGL+S+RL+RRN++STRH+IKKL RR+K L+  VKSCL DCLELYSDAI
Sbjct: 101 EHGRGALDLRRLGLVSLRLVRRNVSSTRHYIKKL-RRNKHLNTLVKSCLTDCLELYSDAI 160

Query: 126 PTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIA 185
           PT+K ARRDY+  RY +   +ISSVMDD STCEDGF EEEGV+SPLT+RN+NAFELSAIA
Sbjct: 161 PTLKDARRDYKGKRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRNYNAFELSAIA 220

Query: 186 LSIINMI 191
           LSIINM+
Sbjct: 221 LSIINML 225

[Close]

BLAST of Sgr025677 vs. NCBI nr
Match: XP_022999944.1 (putative invertase inhibitor [Cucurbita maxima])

HSP 1 Score: 243.8 bits (621), Expect = 1.2e-60
Identity = 135/191 (70.68%), Postives = 156/191 (81.68%), Query Frame = 0


Query: 6   LSSFSLYILPFFFFFLSTFPITEA--SNTSSLIYKTCKTSADQDPNISFNFCSTSLQ--- 65
           +SS SL ILPF FF  S FPIT++  SNTSSLIYKTCKTS+DQDPNISFNFC TSLQ   
Sbjct: 1   MSSPSLSILPFLFFLFSNFPITQSSISNTSSLIYKTCKTSSDQDPNISFNFCLTSLQHGV 60

Query: 66  -AASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELY 125
            A + H R   DLR LGL+S+RL+RRN++STRHHIKKL +R+K L+  VKSCLADCLELY
Sbjct: 61  AATAKHGRTALDLRRLGLISLRLVRRNVSSTRHHIKKL-QRNKHLNTLVKSCLADCLELY 120

Query: 126 SDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFEL 185
           SDAIP +K ARRDY+  RY +   +ISSVMDD STCEDGF EEEGV+SPLT+RN+N FEL
Sbjct: 121 SDAIPILKDARRDYKDRRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRNYNVFEL 180

Query: 186 SAIALSIINMI 191
           SAIALSIINM+
Sbjct: 181 SAIALSIINML 189

[Close]

BLAST of Sgr025677 vs. NCBI nr
Match: XP_022155774.1 (putative invertase inhibitor [Momordica charantia])

HSP 1 Score: 243.4 bits (620), Expect = 1.6e-60
Identity = 139/198 (70.20%), Postives = 159/198 (80.30%), Query Frame = 0


Query: 1   MSPLSLSSFSLYILPFFFFFLSTF-----PITEASNTSSLIYKTCKTSADQDPNISFNFC 60
           MSPLS        + FFF FLST      P   +++ SSLIYKTCK S+ QDPNISFNFC
Sbjct: 1   MSPLS------PFIIFFFVFLSTLPTLTQPYNNSNSISSLIYKTCKISSLQDPNISFNFC 60

Query: 61  STSLQAASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKT---LDPFVKSCL 120
            TSLQ+A+ H+ + ADLR LGLLS+RL+RRN+T+TR HIKKL RR      LDPF+KSCL
Sbjct: 61  LTSLQSAA-HAHQ-ADLRHLGLLSLRLLRRNVTTTRRHIKKLLRRKSNKIRLDPFIKSCL 120

Query: 121 ADCLELYSDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSR 180
            DCLELYSD IPT+K+ARRDYRAGRY++A +EISSVMDDS TCEDGFKEEEGVVSPLTSR
Sbjct: 121 DDCLELYSDDIPTLKRARRDYRAGRYAEANSEISSVMDDSVTCEDGFKEEEGVVSPLTSR 180

Query: 181 NHNAFELSAIALSIINMI 191
           NHNAFELSAIALSIINM+
Sbjct: 181 NHNAFELSAIALSIINML 190

[Close]

BLAST of Sgr025677 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 109.0 bits (271), Expect = 6.1e-23
Identity = 65/183 (35.52%), Postives = 114/183 (62.30%), Query Frame = 0


Query: 8   SFSLYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSR 67
           SFSL I     FF +   + +A  ++ ++  TCK  A + PN++++FC  SL A  +   
Sbjct: 4   SFSLCI-----FFFNLLLLLQAVISADIVQGTCKKVAQRSPNVNYDFCVKSLGA--DPKS 63

Query: 68  RGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVK 127
             ADL+ LG++S  L  ++ +  +  I ++ +    +DP +K  L DC+ LY+DA  +V+
Sbjct: 64  HTADLQGLGVISANLAIQHGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSVQ 123

Query: 128 QARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSII 187
           +A  D+++  Y+ A  ++S+ +DDS TCEDGFKE++G+VSP+T  N +  +L+AI+L+I 
Sbjct: 124 EAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENKDYVQLTAISLAIT 177

Query: 188 NMI 191
            ++
Sbjct: 184 KLL 177

[Close]

BLAST of Sgr025677 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 102.8 bits (255), Expect = 4.4e-21
Identity = 66/184 (35.87%), Postives = 111/184 (60.33%), Query Frame = 0


Query: 8   SFSLYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQA-ASNHS 67
           SFSL I    FF +S          + ++  TCK  A + PN++++FC  SL A   +HS
Sbjct: 4   SFSLCI----FFLIS----------ADIVQGTCKKVAQRSPNVNYDFCVKSLGADPKSHS 63

Query: 68  RRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTV 127
              ADL+ LG++S  L  +  +  +  I ++ +    +DP +K  L DC+ LY+DA  +V
Sbjct: 64  ---ADLQGLGVISANLAIQQGSKIQTFIGRILK--SKVDPALKKYLNDCVGLYADAKSSV 123

Query: 128 KQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSI 187
           ++A  D+++  Y+ A  ++S+ +DDS TCEDGFKE++G+ SP+T  N +  +L+AI+L+I
Sbjct: 124 QEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENKDYVQLTAISLAI 168

Query: 188 INMI 191
             ++
Sbjct: 184 TKLL 168

[Close]

BLAST of Sgr025677 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.6e-07
Identity = 51/180 (28.33%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 11  LYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSRRGA 70
           L  L  F  FL+ F     +   +LI  +CK +  +DP +S++FC  SL      S+   
Sbjct: 4   LVSLVIFSLFLNGF-----ATAQTLIQDSCKKAFAKDPQLSYDFCVNSL-TQDPQSKAAT 63

Query: 71  DLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQAR 130
            L SL L S +     +T+ +  + +  +  +  D  +   L  CL  Y+DA   +  A 
Sbjct: 64  TLESLVLASTKTAAAKITNLKGIVAQDLKDQRYQD--IVEDLKLCLGFYNDANDDLTTAL 123

Query: 131 RDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSIINMI 190
            + ++  Y  A   +S+ +D    CED FKE +   SP+T+ N   F+   I L+  NM+
Sbjct: 124 ANIKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of Sgr025677 vs. ExPASy TrEMBL
Match: A0A0A0KBR8 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080370 PE=4 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 2.0e-61
Identity = 133/193 (68.91%), Postives = 157/193 (81.35%), Query Frame = 0


Query: 6   LSSFSLYILP--FFFFFLSTFPITEAS---NTSSLIYKTCKTSADQDPNISFNFCSTSLQ 65
           +SS S  ILP  F F F S FPIT++S   NTSSLIYKTCK S++QDPNISFNFC TSL+
Sbjct: 1   MSSLSHSILPCLFLFMFFSNFPITQSSNNNNTSSLIYKTCKASSEQDPNISFNFCVTSLK 60

Query: 66  -AASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSK-TLDPFVKSCLADCLEL 125
            AA+ H      LR LGL++I LIR N+++TRHHIKK   ++K   DPFVK CL DCLEL
Sbjct: 61  PAATKHRHGDTSLRRLGLITIYLIRHNMSNTRHHIKKYLHKNKGPRDPFVKLCLTDCLEL 120

Query: 126 YSDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFE 185
           YSDAIPTVKQAR+DY+AGRY+DA  +ISSVMDD STCE+GFKE++GV+SPLT+RNHNAFE
Sbjct: 121 YSDAIPTVKQARKDYKAGRYADANLKISSVMDDCSTCEEGFKEKDGVISPLTNRNHNAFE 180

Query: 186 LSAIALSIINMIC 192
           LSAIALSIINM+C
Sbjct: 181 LSAIALSIINMLC 193

[Close]

BLAST of Sgr025677 vs. ExPASy TrEMBL
Match: A0A6J1KIJ0 (putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494270 PE=4 SV=1)

HSP 1 Score: 243.8 bits (621), Expect = 5.9e-61
Identity = 135/191 (70.68%), Postives = 156/191 (81.68%), Query Frame = 0


Query: 6   LSSFSLYILPFFFFFLSTFPITEA--SNTSSLIYKTCKTSADQDPNISFNFCSTSLQ--- 65
           +SS SL ILPF FF  S FPIT++  SNTSSLIYKTCKTS+DQDPNISFNFC TSLQ   
Sbjct: 1   MSSPSLSILPFLFFLFSNFPITQSSISNTSSLIYKTCKTSSDQDPNISFNFCLTSLQHGV 60

Query: 66  -AASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELY 125
            A + H R   DLR LGL+S+RL+RRN++STRHHIKKL +R+K L+  VKSCLADCLELY
Sbjct: 61  AATAKHGRTALDLRRLGLISLRLVRRNVSSTRHHIKKL-QRNKHLNTLVKSCLADCLELY 120

Query: 126 SDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFEL 185
           SDAIP +K ARRDY+  RY +   +ISSVMDD STCEDGF EEEGV+SPLT+RN+N FEL
Sbjct: 121 SDAIPILKDARRDYKDRRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRNYNVFEL 180

Query: 186 SAIALSIINMI 191
           SAIALSIINM+
Sbjct: 181 SAIALSIINML 189

[Close]

BLAST of Sgr025677 vs. ExPASy TrEMBL
Match: A0A6J1DR96 (putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111022813 PE=4 SV=1)

HSP 1 Score: 243.4 bits (620), Expect = 7.7e-61
Identity = 139/198 (70.20%), Postives = 159/198 (80.30%), Query Frame = 0


Query: 1   MSPLSLSSFSLYILPFFFFFLSTF-----PITEASNTSSLIYKTCKTSADQDPNISFNFC 60
           MSPLS        + FFF FLST      P   +++ SSLIYKTCK S+ QDPNISFNFC
Sbjct: 1   MSPLS------PFIIFFFVFLSTLPTLTQPYNNSNSISSLIYKTCKISSLQDPNISFNFC 60

Query: 61  STSLQAASNHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKT---LDPFVKSCL 120
            TSLQ+A+ H+ + ADLR LGLLS+RL+RRN+T+TR HIKKL RR      LDPF+KSCL
Sbjct: 61  LTSLQSAA-HAHQ-ADLRHLGLLSLRLLRRNVTTTRRHIKKLLRRKSNKIRLDPFIKSCL 120

Query: 121 ADCLELYSDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSR 180
            DCLELYSD IPT+K+ARRDYRAGRY++A +EISSVMDDS TCEDGFKEEEGVVSPLTSR
Sbjct: 121 DDCLELYSDDIPTLKRARRDYRAGRYAEANSEISSVMDDSVTCEDGFKEEEGVVSPLTSR 180

Query: 181 NHNAFELSAIALSIINMI 191
           NHNAFELSAIALSIINM+
Sbjct: 181 NHNAFELSAIALSIINML 190

[Close]

BLAST of Sgr025677 vs. ExPASy TrEMBL
Match: A0A6J1HKN9 (putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464419 PE=4 SV=1)

HSP 1 Score: 242.7 bits (618), Expect = 1.3e-60
Identity = 131/187 (70.05%), Postives = 154/187 (82.35%), Query Frame = 0


Query: 6   LSSFSLYILPFFFFFLSTFPITEA--SNTSSLIYKTCKTSADQDPNISFNFCSTSLQAAS 65
           +SS S+ ILPF FF  S F IT++  SNTSSLIY TCKTS+DQDPNISFNFC TSLQ  +
Sbjct: 1   MSSLSVSILPFLFFLFSNFAITQSSISNTSSLIYNTCKTSSDQDPNISFNFCLTSLQHGA 60

Query: 66  NHSRRGADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAI 125
            H R   DLR LGL+S+RL+RRN++STRH+IKKL RR+K L+  VKSCL DCLELYSDAI
Sbjct: 61  EHGRGALDLRRLGLISLRLVRRNVSSTRHYIKKL-RRNKHLNTLVKSCLTDCLELYSDAI 120

Query: 126 PTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIA 185
           PT+K ARRDY+  RY +   +ISSVMDD STCEDGF EEEGV+SPLT+R++NAFELSAIA
Sbjct: 121 PTLKDARRDYKGRRYFETNLKISSVMDDCSTCEDGF-EEEGVISPLTNRSYNAFELSAIA 180

Query: 186 LSIINMI 191
           LSIINM+
Sbjct: 181 LSIINML 185

[Close]

BLAST of Sgr025677 vs. ExPASy TrEMBL
Match: A0A5A7TEK0 (Invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G00910 PE=4 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.5e-59
Identity = 124/174 (71.26%), Postives = 147/174 (84.48%), Query Frame = 0


Query: 22  STFPITEAS----NTSSLIYKTCKTSADQDPNISFNFCSTSLQ-AASNHSRRGADLRSLG 81
           S FPIT++S    NTSSLIYKTCK S++QDPNISF+FC TSL+ AA+ H      LR LG
Sbjct: 5   SNFPITQSSNNNNNTSSLIYKTCKASSEQDPNISFDFCITSLKPAATKHRHGDTSLRRLG 64

Query: 82  LLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQARRDYRAG 141
           L++I LIR N++STRHHIKKL+R+    DPFVK CL DCLELYSDAIP VKQAR+DY+AG
Sbjct: 65  LMTIYLIRHNMSSTRHHIKKLQRKKGPGDPFVKLCLTDCLELYSDAIPMVKQARKDYKAG 124

Query: 142 RYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSIINMI 191
           RY+DA ++ISSVMDD STCE+GF+E+EGVVSPLT+RNHNAFELSAIALSIINM+
Sbjct: 125 RYADANSKISSVMDDCSTCEEGFEEKEGVVSPLTNRNHNAFELSAIALSIINML 178

[Close]

BLAST of Sgr025677 vs. TAIR 10
Match: AT5G46940.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 8.2e-15
Identity = 55/182 (30.22%), Postives = 91/182 (50.00%), Query Frame = 0


Query: 9   FSLYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSRR 68
           F LY++ FF            +N  +LI  +CK +    P   +N C TSL+  +  ++ 
Sbjct: 3   FLLYLVTFFVL------SNGLANGQTLIRNSCKKATATSPKFKYNLCVTSLE-TNPQAKT 62

Query: 69  GADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQ 128
             DL  L + S +      T+ +  + K+  + K ++      L DCL+LY+DAI ++ +
Sbjct: 63  AKDLAGLVMASTKNAVTKATTLKGTVDKI-IKGKKVNKMTAMPLRDCLQLYTDAIGSLNE 122

Query: 129 ARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSIIN 188
           A    ++  Y    T +S+ MD  STCE GFKE +   SP+T  N N +++  I L+  N
Sbjct: 123 ALAGVKSRNYPTVKTVLSAAMDTPSTCETGFKERK-APSPVTKENDNLYQMILIPLAFTN 175

Query: 189 MI 191
           M+
Sbjct: 183 ML 175

[Close]

BLAST of Sgr025677 vs. TAIR 10
Match: AT5G46970.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 3.4e-13
Identity = 56/168 (33.33%), Postives = 87/168 (51.79%), Query Frame = 0


Query: 11  LYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSRRGA 70
           L+    FF  L+ F I +     SLI   CK +AD++P I +NFC  SL+  +  S+   
Sbjct: 4   LFYFAMFFLLLNAFTIAQ-----SLIQDFCKKAADKNPKIHYNFCVKSLE-ENPQSKTAR 63

Query: 71  DLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQAR 130
            L  L +LS +      TS +  + K+ + ++  + + +  L DCLELYSDA  ++K+A 
Sbjct: 64  SLDRLVMLSTKNAVSKTTSMKGIVDKILKENR-FEMYSEKPLRDCLELYSDATNSLKEAL 123

Query: 131 RDYRAGRYSDAGTEISSVMDDSSTCEDGFKE-EEGVVSPLTSRNHNAF 178
              ++  Y  A   IS+ M    +CE GFKE ++ + SP T  N   F
Sbjct: 124 TIIKSRDYKTANVVISAAMGAPPSCEIGFKEGKKPLKSPFTKDNDVLF 164

[Close]

BLAST of Sgr025677 vs. TAIR 10
Match: AT5G46930.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 67.0 bits (162), Expect = 1.9e-11
Identity = 55/186 (29.57%), Postives = 94/186 (50.54%), Query Frame = 0


Query: 9   FSLYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSRR 68
           FSLY++  FF  L+ F     +   SLI  +CK +  +DP + ++FC  SL+  +  S+ 
Sbjct: 3   FSLYLV-MFFLLLNCF-----ATAQSLIRDSCKKATTKDPKLKYDFCVKSLE-ENPQSKT 62

Query: 69  GADLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQ 128
              L  L  +S +      TS +  + K+ +  K     V+  L DCLELY++AI ++ Q
Sbjct: 63  AKSLEGLVFVSTKNAVSKTTSLKGMVDKILKEDKY---EVERPLLDCLELYTEAIDSLNQ 122

Query: 129 ARRDYRAGRYSDAGTEISSVMDDSSTCEDGF-KEEEGVVSPLTSRNHNAFELSAIALSII 188
           +    ++  Y  A   +S+ MD   +CE  F K ++ V SP T  N   F +  I ++  
Sbjct: 123 SLDTVKSRDYKTATMLMSAAMDAPGSCETKFTKRKKAVKSPFTKENDVLFYMVLIPIAFT 178

Query: 189 NMICLR 194
           +M+ ++
Sbjct: 183 SMLDMK 178

[Close]

BLAST of Sgr025677 vs. TAIR 10
Match: AT5G46960.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-08
Identity = 51/180 (28.33%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 11  LYILPFFFFFLSTFPITEASNTSSLIYKTCKTSADQDPNISFNFCSTSLQAASNHSRRGA 70
           L  L  F  FL+ F     +   +LI  +CK +  +DP +S++FC  SL      S+   
Sbjct: 4   LVSLVIFSLFLNGF-----ATAQTLIQDSCKKAFAKDPQLSYDFCVNSL-TQDPQSKAAT 63

Query: 71  DLRSLGLLSIRLIRRNLTSTRHHIKKLRRRSKTLDPFVKSCLADCLELYSDAIPTVKQAR 130
            L SL L S +     +T+ +  + +  +  +  D  +   L  CL  Y+DA   +  A 
Sbjct: 64  TLESLVLASTKTAAAKITNLKGIVAQDLKDQRYQD--IVEDLKLCLGFYNDANDDLTTAL 123

Query: 131 RDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSRNHNAFELSAIALSIINMI 190
            + ++  Y  A   +S+ +D    CED FKE +   SP+T+ N   F+   I L+  NM+
Sbjct: 124 ANIKSRDYQGANINLSAALDVPGNCEDDFKEAK-KTSPITNENSILFKTILIPLAFTNML 174

[Close]

BLAST of Sgr025677 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 45.4 bits (106), Expect = 5.9e-05
Identity = 26/78 (33.33%), Postives = 41/78 (52.56%), Query Frame = 0


Query: 113 ADCLELYSDAIPTVKQARRDYRAGRYSDAGTEISSVMDDSSTCEDGFKEEEGVVSPLTSR 172
           +DC ELY DA+  ++ A    ++   +   T +S+ M D STC DGF EE G  +PL   
Sbjct: 187 SDCQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGF-EESGEPNPLAYV 246

Query: 173 NHNAFELSAIALSIINMI 191
                ++ +  L+I  +I
Sbjct: 247 ADKLTKMVSNCLAISTLI 263

The following BLAST results are available for this feature:

BLAST of Sgr025677 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004145025.1	4.2e-61	68.91	putative invertase inhibitor [Cucumis sativus]	[more]
XP_023514422.1	9.3e-61	70.68	putative invertase inhibitor [Cucurbita pepo subsp. pepo]	[more]
KAG6593721.1	9.3e-61	70.59	hypothetical protein SDJN03_13197, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022999944.1	1.2e-60	70.68	putative invertase inhibitor [Cucurbita maxima]	[more]
XP_022155774.1	1.6e-60	70.20	putative invertase inhibitor [Momordica charantia]	[more]

BLAST of Sgr025677 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	6.1e-23	35.52	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	4.4e-21	35.87	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	1.6e-07	28.33	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]

BLAST of Sgr025677 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KBR8	2.0e-61	68.91	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G080370 PE=4 S...	[more]
A0A6J1KIJ0	5.9e-61	70.68	putative invertase inhibitor OS=Cucurbita maxima OX=3661 GN=LOC111494270 PE=4 SV...	[more]
A0A6J1DR96	7.7e-61	70.20	putative invertase inhibitor OS=Momordica charantia OX=3673 GN=LOC111022813 PE=4...	[more]
A0A6J1HKN9	1.3e-60	70.05	putative invertase inhibitor OS=Cucurbita moschata OX=3662 GN=LOC111464419 PE=4 ...	[more]
A0A5A7TEK0	2.5e-59	71.26	Invertase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G...	[more]

BLAST of Sgr025677 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G46940.1	8.2e-15	30.22	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46970.1	3.4e-13	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46930.1	1.9e-11	29.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G46960.1	1.1e-08	28.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	5.9e-05	33.33	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 31..186 e-value: 1.9E-28 score: 110.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 9..190 e-value: 2.2E-39 score: 132.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 35..186 e-value: 4.1E-26 score: 92.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 32..191 e-value: 6.8E-36 score: 125.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 33..190
None	No IPR available	PANTHER	PTHR31707:SF314	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR	coord: 6..189
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 6..189
IPR034088	Pla a 1-like	CDD	cd15795	PMEI-Pla_a_1_like	coord: 40..189 e-value: 6.35556E-43 score: 139.037

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr025677.1	Sgr025677.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity