Sgr025667 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025667 vs. NCBI nr
Match: KAE7999221.1 (hypothetical protein FH972_003676 [Carpinus fangiana])

HSP 1 Score: 2023.4 bits (5241), Expect = 0.0e+00
Identity = 1023/1657 (61.74%), Postives = 1220/1657 (73.63%), Query Frame = 0

Query: 14   ATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPNSPSVHFGN 73
            A   PIKTVVVLVQENRSFDH+LGWMKSLN EI+GVT     SNP+ST+EP+S  +++G+
Sbjct: 8    AATYPIKTVVVLVQENRSFDHILGWMKSLNPEINGVTGKE--SNPLSTTEPSSKQIYYGD 67

Query: 74   ASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNAERNSKGMSETVMNGF 133
             S +V PDPGHSIQ IYEQ+FGEPWSE S +K L P M GFAQNAER   G+SETV+NGF
Sbjct: 68   KSVFVVPDPGHSIQAIYEQVFGEPWSEESAAKGLSPNMSGFAQNAERTETGLSETVLNGF 127

Query: 134  KPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQLIGGLPQKT 193
             P+ V VFKELV+EF VCDRWFASVPASTQPNRLY+HSATSHGLS NDTKQLI G+PQKT
Sbjct: 128  LPDNVQVFKELVSEFAVCDRWFASVPASTQPNRLYVHSATSHGLSGNDTKQLIEGMPQKT 187

Query: 194  IFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREGKLPNYVVIE 253
            IFES+ E G +FGIYYQY PATL YRNLRKLKY+ +FH F+++FK+HC EGKLPNYVVIE
Sbjct: 188  IFESVHEAGLSFGIYYQYPPATLYYRNLRKLKYLIHFHDFNLEFKKHCEEGKLPNYVVIE 247

Query: 254  QRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVP 313
            QR+FDL S+P NDDHPSHDVS GQKFIKEVYE LR+SPQWNE+LF+I YDEHGGF+DHVP
Sbjct: 248  QRWFDLLSIPANDDHPSHDVSVGQKFIKEVYETLRASPQWNEMLFIIIYDEHGGFYDHVP 307

Query: 314  TPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVGPQPTSEFEHSS 373
            TP VGVP+PD L+GP PYNFKFDRLGVRVP + +SPWIE GTV+H P GP PTSEFEHSS
Sbjct: 308  TPAVGVPSPDDLIGPAPYNFKFDRLGVRVPAILISPWIERGTVLHGPSGPYPTSEFEHSS 367

Query: 374  IAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLRDVGANDTRRIS 433
            IAATVKKIF LK FLTKRDEWAGTFE VL R SPRTDCPVTL +P KLR+ G  +  ++S
Sbjct: 368  IAATVKKIFNLKDFLTKRDEWAGTFEGVLTRTSPRTDCPVTLGEPAKLRETGPQEEAKLS 427

Query: 434  EFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECEKARENGADE 493
            EFQEELVQLAAVL GD +K++YP KLVENM+V EA  Y + A K F  EC KARE+G DE
Sbjct: 428  EFQEELVQLAAVLNGDHRKDIYPDKLVENMTVGEAAKYVQEAFKKFQDECAKARESGVDE 487

Query: 494  SQIVVCGNQPQPSSKPKSFARSLSKLYFIV-----DFSSFVAFKCTRSSSTMKQ--PPCC 553
             +IVVC       +      R  S L           SSF A     S+S  ++    CC
Sbjct: 488  DEIVVCATTASSLASKSLVHRIFSCLICDAIKRRNSISSFAAEMSDESASGERKQCSCCC 547

Query: 554  ISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGDKVISGDGADNFFKERAISAFPSM 613
            ISHEF R AS +P+KIA IHASGG Q+ +EL        +     D  FKERA S  P +
Sbjct: 548  ISHEFFRAASKNPNKIAVIHASGGAQISKELS-------VDDIDTDKLFKERAKSLSPPV 607

Query: 614  YEGDRCFTYSQLLASVDSLSSRLLPILRDA-ADDHQLITPTAPPRANDGNGEPAKTDRMT 673
            Y+GDRCFTYS +LASVDSLS+RL  IL DA ADD  LI  +        + + AK+   +
Sbjct: 608  YQGDRCFTYSDVLASVDSLSARLRSILLDAVADDPHLIAHSPKGNNTSNHAQMAKSSASS 667

Query: 674  AELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKTRILSVVS 733
                E S E +S  +PKI GIYMPPSVEYI+AVLS+LRCG AFMPLDP+WPK RILS  +
Sbjct: 668  MLRAEQSTEFKSIYVPKIVGIYMPPSVEYIVAVLSVLRCGAAFMPLDPSWPKERILSAAA 727

Query: 734  SSKIDLIIYSGSSF-CEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSANLVFPC 793
            SS +D+II   SSF    GY L D  HWL++ S CS  CF+MEE  + E    ANLV+PC
Sbjct: 728  SSNVDVIIGCASSFGMSSGYQL-DRSHWLLECSSCSVLCFSMEE-CLEECIRPANLVWPC 787

Query: 794  EHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKTSISFID 853
            + G+ RLFCY+MYTSGSTGKPKG+CGTEQGL+NRF WMQ+L+P  G+E+++FKTSISFID
Sbjct: 788  QIGEERLFCYLMYTSGSTGKPKGVCGTEQGLINRFLWMQDLYPLQGEEILMFKTSISFID 847

Query: 854  HIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLPALQRLY 913
            H+QEFL A+LT+  LVIPP  ELK+N++S+V+F+Q Y I++LT+VPSLM+A+LPALQ   
Sbjct: 848  HLQEFLGAILTACPLVIPPFSELKDNMFSVVDFLQVYFINRLTSVPSLMKAILPALQSQS 907

Query: 914  LMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCKRMPMIL 973
                  SL+LL+LSGE+LP+ LW+ L KLLPET+ILN+YGSTEVSGDCTYFDCKR+PMIL
Sbjct: 908  NRGIPTSLKLLVLSGEVLPLALWDKLAKLLPETSILNIYGSTEVSGDCTYFDCKRLPMIL 967

Query: 974  ETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLGGIFSQD 1033
            + + +  VPIG+P+S CDV++V +N   N+GE+ VGG CV  GYYSDST + L       
Sbjct: 968  DMDTLTSVPIGMPLSNCDVLLVGENGTSNQGEIYVGGVCVSCGYYSDSTVMSLDCAKLPQ 1027

Query: 1034 LVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIEDTLREHL 1093
               G S   + SQ+Y  TGDF +RLQSGDLVFLGRKDR++KVNGQRIALEEIED LR H 
Sbjct: 1028 NSVGSSSTEHGSQLYFRTGDFARRLQSGDLVFLGRKDRTVKVNGQRIALEEIEDVLRTHP 1087

Query: 1094 DVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPNSFFFIDS 1153
            DV+ AAVVS +   EL  L AF+VLK+ +  E+FRSSIRSWM++K+   M+PN F F +S
Sbjct: 1088 DVLEAAVVSSKGQWELVALEAFIVLKEERSREIFRSSIRSWMIDKLLSVMLPNHFTFTES 1147

Query: 1154 IPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEVSSDDDFF 1213
            IP+SSSGKVDYELL   T L E   + I    ++D +QV+KKAF+DALMVEEVS DDDFF
Sbjct: 1148 IPVSSSGKVDYELLAGLTSLTEPVQDKIGDMGSSDLLQVVKKAFTDALMVEEVSDDDDFF 1207

Query: 1214 MMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDA--------- 1273
            MMGGNS+ AAH+SH LGVDMR++Y++P+P+KL  ALLEK+G   + + +DA         
Sbjct: 1208 MMGGNSIAAAHLSHNLGVDMRFIYYFPSPSKLYLALLEKRGPGHLHVKKDANWEVNLDEG 1267

Query: 1274 --------------------------------------------DSRKNLKTD------- 1333
                                                        DS  N+ +D       
Sbjct: 1268 KRSTLRSINFEAPDPGIFKPQGSLLRTSVEKNDSNVIVSKRLKVDSNINVTSDSASARDG 1327

Query: 1334 --------------SRCNKVVYEHKYIGNKKCAETLSVKSQRGENGSMKKLWQVHMESCV 1393
                          SRCNKV+YE  Y GNK C  T SVK  R   GSM++ W+VHMESCV
Sbjct: 1328 YVWDSASKLMSCSASRCNKVMYEEGYSGNKICQATWSVKIPRDRKGSMQEFWKVHMESCV 1387

Query: 1394 DASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEGRIECSTAIVGDFSQVVVG 1453
            DASP+VVFK  + YLFIGSHS KF+CV AK+ S+QWE++LEGRIECS AI+GDFSQVVVG
Sbjct: 1388 DASPIVVFKDQDIYLFIGSHSCKFLCVAAKSGSVQWEIKLEGRIECSAAILGDFSQVVVG 1447

Query: 1454 CYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCGSYDHNLYALDYVRHSCVY 1513
            CYKGKIYFL+FS G I WTFQT GEVKSQPVVD    L+WCGS+DHNLYALDY  H CVY
Sbjct: 1448 CYKGKIYFLDFSNGNICWTFQTSGEVKSQPVVDIHNQLVWCGSHDHNLYALDYKNHCCVY 1507

Query: 1514 KLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFSTLWHYDLEAPVFGSLVIDH 1573
             +PCGGS+YGSPAID V + LYVASTSGRMTA+  K+ PF+ LW ++ E PVFGSL I+ 
Sbjct: 1508 MVPCGGSIYGSPAIDEVHNTLYVASTSGRMTAISTKSLPFNILWLHEFEVPVFGSLAINS 1567

Query: 1574 LNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSAIPSQVLICSRNGSIHSFE 1588
            LN NVICCLVDGHV+ALDSSGS+ W+ +TGGPIFAG CIS+A+PSQ LICSR+G I+S E
Sbjct: 1568 LNGNVICCLVDGHVLALDSSGSILWKYRTGGPIFAGPCISAALPSQGLICSRDGGIYSLE 1627

BLAST of Sgr025667 vs. NCBI nr
Match: KAA8520349.1 (hypothetical protein F0562_014605 [Nyssa sinensis])

HSP 1 Score: 1899.8 bits (4920), Expect = 0.0e+00
Identity = 997/1720 (57.97%), Postives = 1197/1720 (69.59%), Query Frame = 0

Query: 1    MAPEITGTGGDGKATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPIS 60
            MA EIT        +P PIKT+VVLVQENRSFDHMLGWMKSLN EI+GVT     SNP+S
Sbjct: 1    MASEIT--------SPYPIKTIVVLVQENRSFDHMLGWMKSLNPEINGVTGTE--SNPLS 60

Query: 61   TSEPNSPSVHFGNASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNAER 120
            TS+ NS  + F + S YVDPDPGHSIQD+YEQIFG PWS+   SK LQP M GFAQNAER
Sbjct: 61   TSDRNSKRIFFRDRSAYVDPDPGHSIQDMYEQIFGMPWSQELSSKKLQPTMEGFAQNAER 120

Query: 121  NSKGMSETVMNGFKPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSN 180
               GMS+TVM GFKP+ V V+KELV+EF VCD+WFA+VPASTQPNRLY+HSATSHG +SN
Sbjct: 121  IEAGMSDTVMKGFKPDDVPVYKELVSEFAVCDQWFAAVPASTQPNRLYVHSATSHGATSN 180

Query: 181  DTKQLIGGLPQKTIFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRH 240
            DT QLI G PQKTIFES+DE G+ FGIYYQY PATL YRNLRKLKY+KNFH FD+DFKRH
Sbjct: 181  DTSQLIEGHPQKTIFESMDEAGYTFGIYYQYPPATLFYRNLRKLKYIKNFHQFDLDFKRH 240

Query: 241  CREGKLPNYVVIEQRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLI 300
            C EGKLPNYVV+EQRYFDL  LPGNDDHPSHDV EGQKF+KEVYEALR+SPQWNE+LF+I
Sbjct: 241  CEEGKLPNYVVLEQRYFDLKVLPGNDDHPSHDVFEGQKFVKEVYEALRASPQWNEMLFII 300

Query: 301  TYDEHGGFFDHVPTPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRP 360
             YDEHGGF+DHVPTPV GVP+PDG++GP PYNF+FDRLGVRVP + +SPWIEPGTV+HRP
Sbjct: 301  IYDEHGGFYDHVPTPVTGVPSPDGIMGPEPYNFQFDRLGVRVPALMISPWIEPGTVLHRP 360

Query: 361  VGPQPTSEFEHSSIAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVK 420
             GP P+SEFEHSS+ ATVKKIF L +FLT+RD WAGTFE+VLNR+SPRTDCPVTL +PVK
Sbjct: 361  SGPYPSSEFEHSSVPATVKKIFNLNEFLTRRDAWAGTFEVVLNRKSPRTDCPVTLPEPVK 420

Query: 421  LRDVGANDTRRISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFL 480
            LR+  A +  ++SEFQEELVQ+AA L GD +K++YP +LVENM+VSEAV Y  NA K FL
Sbjct: 421  LREAEAEENGKLSEFQEELVQMAATLCGDHRKDIYPHRLVENMTVSEAVEYVNNAFKKFL 480

Query: 481  HECEKARENGADESQIVVCGNQPQP-------------------SSKPKSFARSLS---- 540
             ECE AR +GADES I V  +QP+P                   S++PK  A S+     
Sbjct: 481  DECESARASGADESDICVPRDQPKPAESKSVASKLFSCLAEPILSTRPKIPAHSVQLKRR 540

Query: 541  ---------KLYFIVDFSSFVAFKCTRSSSTMK---------------QPPCCISHEFQR 600
                     K  F      F  ++ T S S +                   CCISHEF +
Sbjct: 541  RSNSVSDHLKRKFASGGRIFATYQRTGSPSFLAGKRSSFSLCSLQLRWLAGCCISHEFFK 600

Query: 601  VASAHPDKIAAIHASGGVQL---FRELHGGGGDKV-ISGDGADNFFK--ERAISAFPSMY 660
             AS +P K+A IHA GG  +   FR  H  G D + IS    DNF        S+   +Y
Sbjct: 601  AASKNPSKVAVIHACGGANIAREFRNHHTIGNDNITISEIDYDNFVNGLNTESSSHSPVY 660

Query: 661  EGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEPA----KTDR 720
            EGDRCFT+S++LASVDSLSSRL  IL   +D H +     P   N  + +P         
Sbjct: 661  EGDRCFTFSEILASVDSLSSRLRHILDGGSDPHLI----KPATGNFPSEQPVDVHISESN 720

Query: 721  MTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKTRILSV 780
             ++   E S E +    PKI GIYM PSVEY+IAVLS+LRCG AFMPLDP WPK RILSV
Sbjct: 721  SSSPGVEQSTEYQHMYTPKILGIYMVPSVEYVIAVLSVLRCGEAFMPLDPLWPKERILSV 780

Query: 781  VSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSANLVFP 840
            VSSS +DLII   SSF     H  D  HWLV  S C     +M+ N ++E   S+ LV+P
Sbjct: 781  VSSSNVDLIIGCQSSFDGSWCHELDKSHWLVDCSSCPVLFISMKAN-LQEKFGSSYLVWP 840

Query: 841  CEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKTSISFI 900
            CE G+ R FCY+MYTSGSTGKPKG+CGTE GLLNRF WMQEL P  G+E++ FKTSISFI
Sbjct: 841  CEKGRLRSFCYLMYTSGSTGKPKGVCGTEPGLLNRFMWMQELHPLLGEEILFFKTSISFI 900

Query: 901  DHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLPALQRL 960
            DH+QEF+ A+LT+  LVIPP  EL+ENL+ +++F+QAYSIS+L AVPSLMRAVLPALQ+ 
Sbjct: 901  DHLQEFVGALLTTCTLVIPPFNELRENLFYMIDFLQAYSISRLIAVPSLMRAVLPALQKP 960

Query: 961  YLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCKRMPMI 1020
            Y  + + SL+LL+LSGE+LP+ LW+ L+KLLP+TTILNLYGSTEVSGDCTYFDCKR+PMI
Sbjct: 961  YNTRIQSSLKLLVLSGEVLPLSLWDMLYKLLPKTTILNLYGSTEVSGDCTYFDCKRLPMI 1020

Query: 1021 LETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLGGI-FS 1080
            LE+E ++ VPIG+PIS CDVV+V + ++ N+GE+ VGG CV +GY  D   +    +   
Sbjct: 1021 LESEDLSSVPIGMPISNCDVVLVGE-ESPNQGEIYVGGICVAAGYLCDPYIMQQDFVKLP 1080

Query: 1081 QDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIEDTLRE 1140
            QD     S + +  Q Y +TGDF +RLQSGD VF+GRKDR++KVNGQRIALEEIE TLR 
Sbjct: 1081 QDFCCDCSISEHGRQNYFNTGDFARRLQSGDFVFIGRKDRTVKVNGQRIALEEIESTLRG 1140

Query: 1141 HLDVVNAAVVSGRSDRELEYLVAFLVLKDNKK-SEVFRSSIRSWMVEKVPLAMIPNSFFF 1200
            H DVV+AAVVS + + E+  + A+LV+K   +  E+ RSSIR                  
Sbjct: 1141 HPDVVDAAVVSHKDEGEVMLVDAYLVIKQKDECGEILRSSIR------------------ 1200

Query: 1201 IDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEVSSDD 1260
                     GKVDY LL   T         ID   +  F+Q IKKAF DALMVE VS+DD
Sbjct: 1201 ---------GKVDYSLLASLTFSMTHVQNEIDEIQSTSFLQDIKKAFCDALMVEMVSNDD 1260

Query: 1261 DFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIID--ISRDADSRK 1320
            DFF MGGNS++AAHVSH LG+DMR LY +P+P  L  ALL+K G   +D  +  DA+   
Sbjct: 1261 DFFAMGGNSISAAHVSHNLGIDMRLLYIFPSPLMLQLALLQKIGLCNVDVRVRTDANWGV 1320

Query: 1321 NLKTD------------------------------------------------------- 1380
            NLKT                                                        
Sbjct: 1321 NLKTHVDSTLLSFDSKTPNLYSSKSRGRFSSTLHEKNDNYPVKCLKVDSKLHLNSKGIGH 1380

Query: 1381 -----------------SRCNKVVYEHKYIGNKKCAETLSVKSQRGENGSMKKLWQVHME 1440
                             SRCNKV+YE +  GN     T S +  R + G+M +LW+VH+ 
Sbjct: 1381 GDGYPWYSNSIHMACSFSRCNKVIYEGENAGNSLFQATWSAEIPRDKKGAMLELWKVHLG 1440

Query: 1441 SCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEGRIECSTAIVGDFSQV 1500
            SCVDASP+VVFK  +T+LFIGSHS KF+C++AK+  +QWE++LEGRIECS AI+GDFSQV
Sbjct: 1441 SCVDASPMVVFKDQDTFLFIGSHSHKFLCINAKSGFVQWEIKLEGRIECSAAILGDFSQV 1500

Query: 1501 VVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCGSYDHNLYALDYVRHS 1560
            VVGCY+G IYFL+F  G I WTFQTCGEVKSQP+VD  R+L+WCGSYDHNLYALDY  + 
Sbjct: 1501 VVGCYQGNIYFLDFLDGKIHWTFQTCGEVKSQPLVDKCRSLVWCGSYDHNLYALDYKNYC 1560

Query: 1561 CVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFSTLWHYDLEAPVFGSLV 1588
            CVYKLPCGGS+YGSPAID V   LYVASTSGR+TA+ IKA PFS LW  +LE PVFGSL 
Sbjct: 1561 CVYKLPCGGSIYGSPAIDEVHDTLYVASTSGRITAIYIKALPFSKLWLRELETPVFGSLS 1620

BLAST of Sgr025667 vs. NCBI nr
Match: XP_022155733.1 (putative acyl-activating enzyme 19 isoform X2 [Momordica charantia])

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 900/1135 (79.30%), Postives = 947/1135 (83.44%), Query Frame = 0

Query: 540  MKQPPCCISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGD-KVISGDGADNFFKER 599
            MKQPPCCI HEFQRV+SAHP KIA IHASGGVQLFR+LHGGGGD  +ISGDGADNFFKER
Sbjct: 1    MKQPPCCIFHEFQRVSSAHPHKIAVIHASGGVQLFRQLHGGGGDSNIISGDGADNFFKER 60

Query: 600  AISAFPSMYEGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEP 659
            AISAFPSMYEGDR FTYS LLASVDSLSSRLL                   RANDGNG  
Sbjct: 61   AISAFPSMYEGDRFFTYSHLLASVDSLSSRLL------------------IRANDGNGFS 120

Query: 660  AKTDRMTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKT 719
                        +S  LE +NIPKIFGIYMPPSVEYI+AVLS+LRCGGAFMPLDPAWPK+
Sbjct: 121  ----------EGSSTGLEGANIPKIFGIYMPPSVEYIVAVLSVLRCGGAFMPLDPAWPKS 180

Query: 720  RILSVVSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSA 779
            RILSVVSSSK++LIIYSGSSFCEDGYHLSDGLHWL+QSSGC TFCF MEE+ I+EHNSS 
Sbjct: 181  RILSVVSSSKVELIIYSGSSFCEDGYHLSDGLHWLLQSSGCPTFCFNMEESFIQEHNSSV 240

Query: 780  NLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKT 839
            +LVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFP SG+EL+LFKT
Sbjct: 241  DLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPCSGEELLLFKT 300

Query: 840  SISFIDHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLP 899
             ISFIDHIQEFLSA+LTSSALVIPPMKELKE L S+VNFIQAYSISKLTAVPSLMRAVLP
Sbjct: 301  PISFIDHIQEFLSAILTSSALVIPPMKELKETLCSVVNFIQAYSISKLTAVPSLMRAVLP 360

Query: 900  ALQRLYLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCK 959
            A QRLY+MQNRCSLRLLILSGEIL IQLW AL KLLPETTILNLYGSTEVSGDCTYFDCK
Sbjct: 361  AFQRLYVMQNRCSLRLLILSGEILSIQLWKALLKLLPETTILNLYGSTEVSGDCTYFDCK 420

Query: 960  RMPMILETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLG 1019
            RMP ILETEAIN VPIGVPIS CDVVVV +NDA N+GELCVGGPCVCSGYYSDSTFLPL 
Sbjct: 421  RMPRILETEAINTVPIGVPISHCDVVVVGENDAPNQGELCVGGPCVCSGYYSDSTFLPLD 480

Query: 1020 GI-FSQDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 1079
            G   SQ LV+GGS N NC +IYI TGDFV+RLQSGDLVFLGRKDRSIKVNGQRIALEEIE
Sbjct: 481  GTKLSQGLVNGGSLNENC-KIYIRTGDFVRRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 540

Query: 1080 DTLREHLDVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPN 1139
            D L EH DVVNAA VS RSDRELEYLVAFLVLKDNKKSEVF+ S+RSWMV+KVPLAMIPN
Sbjct: 541  DALMEHPDVVNAAAVSSRSDRELEYLVAFLVLKDNKKSEVFK-SVRSWMVDKVPLAMIPN 600

Query: 1140 SFFFIDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEV 1199
             F  +DSIPMSSSGKVDYEL+MHS PLWE  HEN D T  NDFMQVIKK FSD LMVEEV
Sbjct: 601  RFICVDSIPMSSSGKVDYELVMHSYPLWEHVHENFDETQENDFMQVIKKVFSDVLMVEEV 660

Query: 1200 SSDDDFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDADS 1259
            SS+DDFFMMGGNS+TAAHVSHKLGVD+RWLYHYP+PAKLLTALLEKKGSDIIDISRD DS
Sbjct: 661  SSNDDFFMMGGNSITAAHVSHKLGVDIRWLYHYPSPAKLLTALLEKKGSDIIDISRDVDS 720

Query: 1260 RKNLKTD----------------------------------------------------- 1319
            RKNL+TD                                                     
Sbjct: 721  RKNLRTDKWNKFSFEGSEILNPFDLKEGGNFGKRKQVQSNETLSRVAIPRNDNSSISKHY 780

Query: 1320 --------------------------------SRCNKVVYEHKYIGNKKCAETLSVKSQR 1379
                                            SRCNKVVYEHKYIGN +CAETLSVKSQR
Sbjct: 781  KAVSDFSVNLEHISQVGGHLWNSLLTSMSCAFSRCNKVVYEHKYIGNNECAETLSVKSQR 840

Query: 1380 GENGSMKKLWQVHMESCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEG 1439
            GE GSMKK WQVHMESCVDASPL+VFKHP  YLFIGSHSQKFVCVDAK ASLQWE+RLEG
Sbjct: 841  GEYGSMKKFWQVHMESCVDASPLLVFKHPCIYLFIGSHSQKFVCVDAKTASLQWEIRLEG 900

Query: 1440 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCG 1499
            RIECSTAIVGDFSQVVVGCY+GKIYFLEFSTGII WTFQTCGEVKSQPVVDS RNLIWCG
Sbjct: 901  RIECSTAIVGDFSQVVVGCYEGKIYFLEFSTGIIHWTFQTCGEVKSQPVVDSQRNLIWCG 960

Query: 1500 SYDHNLYALDYVRHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFST 1559
            SYDHNLYALDYVRH+CVYKLPCGGS+YGSPAIDGVQHRLYVASTSGR++ALLIKA PF T
Sbjct: 961  SYDHNLYALDYVRHTCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRISALLIKAFPFGT 1020

Query: 1560 LWHYDLEAPVFGSLVIDHLNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSA 1588
             WHYDLEAPVFGSLVID LNRNVICCLV+GHVVALDSSGSV WRCKTGGPIFAGACISSA
Sbjct: 1021 FWHYDLEAPVFGSLVIDPLNRNVICCLVNGHVVALDSSGSVLWRCKTGGPIFAGACISSA 1080

BLAST of Sgr025667 vs. NCBI nr
Match: XP_022155736.1 (putative acyl-activating enzyme 19 isoform X4 [Momordica charantia])

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 900/1135 (79.30%), Postives = 947/1135 (83.44%), Query Frame = 0

Query: 540  MKQPPCCISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGD-KVISGDGADNFFKER 599
            MKQPPCCI HEFQRV+SAHP KIA IHASGGVQLFR+LHGGGGD  +ISGDGADNFFKER
Sbjct: 1    MKQPPCCIFHEFQRVSSAHPHKIAVIHASGGVQLFRQLHGGGGDSNIISGDGADNFFKER 60

Query: 600  AISAFPSMYEGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEP 659
            AISAFPSMYEGDR FTYS LLASVDSLSSRLL                   RANDGNG  
Sbjct: 61   AISAFPSMYEGDRFFTYSHLLASVDSLSSRLL------------------IRANDGNGFS 120

Query: 660  AKTDRMTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKT 719
                        +S  LE +NIPKIFGIYMPPSVEYI+AVLS+LRCGGAFMPLDPAWPK+
Sbjct: 121  ----------EGSSTGLEGANIPKIFGIYMPPSVEYIVAVLSVLRCGGAFMPLDPAWPKS 180

Query: 720  RILSVVSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSA 779
            RILSVVSSSK++LIIYSGSSFCEDGYHLSDGLHWL+QSSGC TFCF MEE+ I+EHNSS 
Sbjct: 181  RILSVVSSSKVELIIYSGSSFCEDGYHLSDGLHWLLQSSGCPTFCFNMEESFIQEHNSSV 240

Query: 780  NLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKT 839
            +LVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFP SG+EL+LFKT
Sbjct: 241  DLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPCSGEELLLFKT 300

Query: 840  SISFIDHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLP 899
             ISFIDHIQEFLSA+LTSSALVIPPMKELKE L S+VNFIQAYSISKLTAVPSLMRAVLP
Sbjct: 301  PISFIDHIQEFLSAILTSSALVIPPMKELKETLCSVVNFIQAYSISKLTAVPSLMRAVLP 360

Query: 900  ALQRLYLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCK 959
            A QRLY+MQNRCSLRLLILSGEIL IQLW AL KLLPETTILNLYGSTEVSGDCTYFDCK
Sbjct: 361  AFQRLYVMQNRCSLRLLILSGEILSIQLWKALLKLLPETTILNLYGSTEVSGDCTYFDCK 420

Query: 960  RMPMILETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLG 1019
            RMP ILETEAIN VPIGVPIS CDVVVV +NDA N+GELCVGGPCVCSGYYSDSTFLPL 
Sbjct: 421  RMPRILETEAINTVPIGVPISHCDVVVVGENDAPNQGELCVGGPCVCSGYYSDSTFLPLD 480

Query: 1020 GI-FSQDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 1079
            G   SQ LV+GGS N NC +IYI TGDFV+RLQSGDLVFLGRKDRSIKVNGQRIALEEIE
Sbjct: 481  GTKLSQGLVNGGSLNENC-KIYIRTGDFVRRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 540

Query: 1080 DTLREHLDVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPN 1139
            D L EH DVVNAA VS RSDRELEYLVAFLVLKDNKKSEVF+ S+RSWMV+KVPLAMIPN
Sbjct: 541  DALMEHPDVVNAAAVSSRSDRELEYLVAFLVLKDNKKSEVFK-SVRSWMVDKVPLAMIPN 600

Query: 1140 SFFFIDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEV 1199
             F  +DSIPMSSSGKVDYEL+MHS PLWE  HEN D T  NDFMQVIKK FSD LMVEEV
Sbjct: 601  RFICVDSIPMSSSGKVDYELVMHSYPLWEHVHENFDETQENDFMQVIKKVFSDVLMVEEV 660

Query: 1200 SSDDDFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDADS 1259
            SS+DDFFMMGGNS+TAAHVSHKLGVD+RWLYHYP+PAKLLTALLEKKGSDIIDISRD DS
Sbjct: 661  SSNDDFFMMGGNSITAAHVSHKLGVDIRWLYHYPSPAKLLTALLEKKGSDIIDISRDVDS 720

Query: 1260 RKNLKTD----------------------------------------------------- 1319
            RKNL+TD                                                     
Sbjct: 721  RKNLRTDKWNKFSFEGSEILNPFDLKEGGNFGKRKQVQSNETLSRVAIPRNDNSSISKHY 780

Query: 1320 --------------------------------SRCNKVVYEHKYIGNKKCAETLSVKSQR 1379
                                            SRCNKVVYEHKYIGN +CAETLSVKSQR
Sbjct: 781  KAVSDFSVNLEHISQVGGHLWNSLLTSMSCAFSRCNKVVYEHKYIGNNECAETLSVKSQR 840

Query: 1380 GENGSMKKLWQVHMESCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEG 1439
            GE GSMKK WQVHMESCVDASPL+VFKHP  YLFIGSHSQKFVCVDAK ASLQWE+RLEG
Sbjct: 841  GEYGSMKKFWQVHMESCVDASPLLVFKHPCIYLFIGSHSQKFVCVDAKTASLQWEIRLEG 900

Query: 1440 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCG 1499
            RIECSTAIVGDFSQVVVGCY+GKIYFLEFSTGII WTFQTCGEVKSQPVVDS RNLIWCG
Sbjct: 901  RIECSTAIVGDFSQVVVGCYEGKIYFLEFSTGIIHWTFQTCGEVKSQPVVDSQRNLIWCG 960

Query: 1500 SYDHNLYALDYVRHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFST 1559
            SYDHNLYALDYVRH+CVYKLPCGGS+YGSPAIDGVQHRLYVASTSGR++ALLIKA PF T
Sbjct: 961  SYDHNLYALDYVRHTCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRISALLIKAFPFGT 1020

Query: 1560 LWHYDLEAPVFGSLVIDHLNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSA 1588
             WHYDLEAPVFGSLVID LNRNVICCLV+GHVVALDSSGSV WRCKTGGPIFAGACISSA
Sbjct: 1021 FWHYDLEAPVFGSLVIDPLNRNVICCLVNGHVVALDSSGSVLWRCKTGGPIFAGACISSA 1080

BLAST of Sgr025667 vs. NCBI nr
Match: XP_022155734.1 (putative acyl-activating enzyme 19 isoform X3 [Momordica charantia] >XP_022155735.1 putative acyl-activating enzyme 19 isoform X3 [Momordica charantia])

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 900/1135 (79.30%), Postives = 947/1135 (83.44%), Query Frame = 0

Query: 540  MKQPPCCISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGD-KVISGDGADNFFKER 599
            MKQPPCCI HEFQRV+SAHP KIA IHASGGVQLFR+LHGGGGD  +ISGDGADNFFKER
Sbjct: 1    MKQPPCCIFHEFQRVSSAHPHKIAVIHASGGVQLFRQLHGGGGDSNIISGDGADNFFKER 60

Query: 600  AISAFPSMYEGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEP 659
            AISAFPSMYEGDR FTYS LLASVDSLSSRLL                   RANDGNG  
Sbjct: 61   AISAFPSMYEGDRFFTYSHLLASVDSLSSRLL------------------IRANDGNGFS 120

Query: 660  AKTDRMTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKT 719
                        +S  LE +NIPKIFGIYMPPSVEYI+AVLS+LRCGGAFMPLDPAWPK+
Sbjct: 121  ----------EGSSTGLEGANIPKIFGIYMPPSVEYIVAVLSVLRCGGAFMPLDPAWPKS 180

Query: 720  RILSVVSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSA 779
            RILSVVSSSK++LIIYSGSSFCEDGYHLSDGLHWL+QSSGC TFCF MEE+ I+EHNSS 
Sbjct: 181  RILSVVSSSKVELIIYSGSSFCEDGYHLSDGLHWLLQSSGCPTFCFNMEESFIQEHNSSV 240

Query: 780  NLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKT 839
            +LVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFP SG+EL+LFKT
Sbjct: 241  DLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPCSGEELLLFKT 300

Query: 840  SISFIDHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLP 899
             ISFIDHIQEFLSA+LTSSALVIPPMKELKE L S+VNFIQAYSISKLTAVPSLMRAVLP
Sbjct: 301  PISFIDHIQEFLSAILTSSALVIPPMKELKETLCSVVNFIQAYSISKLTAVPSLMRAVLP 360

Query: 900  ALQRLYLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCK 959
            A QRLY+MQNRCSLRLLILSGEIL IQLW AL KLLPETTILNLYGSTEVSGDCTYFDCK
Sbjct: 361  AFQRLYVMQNRCSLRLLILSGEILSIQLWKALLKLLPETTILNLYGSTEVSGDCTYFDCK 420

Query: 960  RMPMILETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLG 1019
            RMP ILETEAIN VPIGVPIS CDVVVV +NDA N+GELCVGGPCVCSGYYSDSTFLPL 
Sbjct: 421  RMPRILETEAINTVPIGVPISHCDVVVVGENDAPNQGELCVGGPCVCSGYYSDSTFLPLD 480

Query: 1020 GI-FSQDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 1079
            G   SQ LV+GGS N NC +IYI TGDFV+RLQSGDLVFLGRKDRSIKVNGQRIALEEIE
Sbjct: 481  GTKLSQGLVNGGSLNENC-KIYIRTGDFVRRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 540

Query: 1080 DTLREHLDVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPN 1139
            D L EH DVVNAA VS RSDRELEYLVAFLVLKDNKKSEVF+ S+RSWMV+KVPLAMIPN
Sbjct: 541  DALMEHPDVVNAAAVSSRSDRELEYLVAFLVLKDNKKSEVFK-SVRSWMVDKVPLAMIPN 600

Query: 1140 SFFFIDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEV 1199
             F  +DSIPMSSSGKVDYEL+MHS PLWE  HEN D T  NDFMQVIKK FSD LMVEEV
Sbjct: 601  RFICVDSIPMSSSGKVDYELVMHSYPLWEHVHENFDETQENDFMQVIKKVFSDVLMVEEV 660

Query: 1200 SSDDDFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDADS 1259
            SS+DDFFMMGGNS+TAAHVSHKLGVD+RWLYHYP+PAKLLTALLEKKGSDIIDISRD DS
Sbjct: 661  SSNDDFFMMGGNSITAAHVSHKLGVDIRWLYHYPSPAKLLTALLEKKGSDIIDISRDVDS 720

Query: 1260 RKNLKTD----------------------------------------------------- 1319
            RKNL+TD                                                     
Sbjct: 721  RKNLRTDKWNKFSFEGSEILNPFDLKEGGNFGKRKQVQSNETLSRVAIPRNDNSSISKHY 780

Query: 1320 --------------------------------SRCNKVVYEHKYIGNKKCAETLSVKSQR 1379
                                            SRCNKVVYEHKYIGN +CAETLSVKSQR
Sbjct: 781  KAVSDFSVNLEHISQVGGHLWNSLLTSMSCAFSRCNKVVYEHKYIGNNECAETLSVKSQR 840

Query: 1380 GENGSMKKLWQVHMESCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEG 1439
            GE GSMKK WQVHMESCVDASPL+VFKHP  YLFIGSHSQKFVCVDAK ASLQWE+RLEG
Sbjct: 841  GEYGSMKKFWQVHMESCVDASPLLVFKHPCIYLFIGSHSQKFVCVDAKTASLQWEIRLEG 900

Query: 1440 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCG 1499
            RIECSTAIVGDFSQVVVGCY+GKIYFLEFSTGII WTFQTCGEVKSQPVVDS RNLIWCG
Sbjct: 901  RIECSTAIVGDFSQVVVGCYEGKIYFLEFSTGIIHWTFQTCGEVKSQPVVDSQRNLIWCG 960

Query: 1500 SYDHNLYALDYVRHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFST 1559
            SYDHNLYALDYVRH+CVYKLPCGGS+YGSPAIDGVQHRLYVASTSGR++ALLIKA PF T
Sbjct: 961  SYDHNLYALDYVRHTCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRISALLIKAFPFGT 1020

Query: 1560 LWHYDLEAPVFGSLVIDHLNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSA 1588
             WHYDLEAPVFGSLVID LNRNVICCLV+GHVVALDSSGSV WRCKTGGPIFAGACISSA
Sbjct: 1021 FWHYDLEAPVFGSLVIDPLNRNVICCLVNGHVVALDSSGSVLWRCKTGGPIFAGACISSA 1080

BLAST of Sgr025667 vs. ExPASy Swiss-Prot
Match: F4K1G2 (Putative acyl-activating enzyme 19 OS=Arabidopsis thaliana OX=3702 GN=At5g35930 PE=2 SV=1)

HSP 1 Score: 958.7 bits (2477), Expect = 7.9e-278
Identity = 497/984 (50.51%), Postives = 644/984 (65.45%), Query Frame = 0

Query: 680  IPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKTRILSVVSSSKIDLIIYSGSSF 739
            +PK+  +YMPPSVEY+I+V S+LRCG AF+PLDP+WP+ R+LS++SSS I L+I  G S 
Sbjct: 1    MPKVVALYMPPSVEYVISVFSVLRCGEAFLPLDPSWPRERVLSLISSSNISLVIACGLSS 60

Query: 740  CEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSANLVFPCEHGKGRLFCYVMYTS 799
             E         HWLV+ + C    F+M+E  +      ++ V+PC+  + R FCY+MYTS
Sbjct: 61   VES--------HWLVERNVCPVLLFSMDEK-LSVETGCSSFVWPCKKERQRKFCYLMYTS 120

Query: 800  GSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKTSISFIDHIQEFLSAMLTSSAL 859
            GSTGKPKG+CGTEQGLLNRF WMQEL+P  G++   FKTS+ FIDHIQEFL A+L+S+AL
Sbjct: 121  GSTGKPKGVCGTEQGLLNRFLWMQELYPVVGEQRFAFKTSVGFIDHIQEFLGAILSSTAL 180

Query: 860  VIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLPALQ-RLYLMQNRCSLRLLILS 919
            VIPP   LKEN+ SI++F++ YSIS+L AVPS++RA+LP LQ R +  + +  L+L++LS
Sbjct: 181  VIPPFTLLKENMISIIDFLEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQSCLKLVVLS 240

Query: 920  GEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCKRMPMILETEAINIVPIGVPI 979
            GE  P+ LW++L  LLPET  LNLYGSTEVSGDCTYFDC  +P +L+TE I  VPIG  I
Sbjct: 241  GEPFPVSLWDSLHSLLPETCFLNLYGSTEVSGDCTYFDCSELPRLLKTEEIGSVPIGKSI 300

Query: 980  SQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLGGIFSQDLV--HGGSF----- 1039
            S C VV++ D D   EGE+CV G C+  GY   S       I S+  V  H  S      
Sbjct: 301  SNCKVVLLGDEDKPYEGEICVSGLCLSQGYMHSS-------IESEGYVKLHNNSLCNHLT 360

Query: 1040 NANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIEDTLREHLDVVNAAV 1099
            N   SQ+Y  TGD+ ++L SGDL+F+GR+DR++K+NG+R+ALEEIE TL  + D+  A V
Sbjct: 361  NDCGSQLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVV 420

Query: 1100 VSGRSDRELEYLVAFLVL-KDNKKSEVFRSSIRSWMVEKVPLAMIPNSFFFIDSIPMSSS 1159
            +  R + EL  L AF+VL K++  S+    SIR+WM  K+P  MIPN F  ++ +P++SS
Sbjct: 421  LLSRDETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSS 480

Query: 1160 GKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEVSSDDDFFMMGGNS 1219
            GKVDYE L           + +     N  +Q IKKA  DAL+V+EVS DDDFF +GG+S
Sbjct: 481  GKVDYEALARLKCPTTGAQDMMQSNGTNSLLQNIKKAVCDALLVKEVSDDDDFFAIGGDS 540

Query: 1220 LTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDA--------------- 1279
            L AAH+SH LG+DMR +Y + +P++LL  L EK+G    D+  +                
Sbjct: 541  LAAAHLSHSLGIDMRLIYQFRSPSRLLIYLSEKEGKLREDMQHNTTQKLDHKIESQNGNG 600

Query: 1280 --------------------------DSRKNLKTD------------------------- 1339
                                      +S K LK D                         
Sbjct: 601  LVSRTVPLHSGVTSGPTPSKLQCEKNNSPKRLKIDYEKFSPKRMKENKLWDSGFSQIQCA 660

Query: 1340 -SRCNKVVYEHKYIGNKKCAETLSVKSQRGENGSMKKLWQVHMESCVDASPLVVFKHPNT 1399
             SRCNKV         +   E  S++  R +  SM+++W+VHMESCVDASPLVV K   T
Sbjct: 661  FSRCNKVHSPESCSNEEANREYWSLEIPRNQMVSMQEIWKVHMESCVDASPLVVLKDSKT 720

Query: 1400 YLFIGSHSQKFVCVDAKNASLQWEMRLEGRIECSTAIVGDFSQVVVGCYKGKIYFLEFST 1459
            YLFIGSHS+KF C+DAK+ S+ WE  LEGRIE S  +VGDFSQVV+GCYKGK+YFL+FST
Sbjct: 721  YLFIGSHSRKFSCIDAKSGSMYWETILEGRIEGSAMVVGDFSQVVIGCYKGKLYFLDFST 780

Query: 1460 GIIQWTFQTCGEVKSQPVVDSDRNLIWCGSYDHNLYALDYVRHSCVYKLPCGGSLYGSPA 1519
            G + W FQ CGE+K QPVVD+   LIWCGS+DH LYALDY    CVYKL CGGS++ SPA
Sbjct: 781  GSLCWKFQACGEIKCQPVVDTSSQLIWCGSHDHTLYALDYRSQCCVYKLQCGGSIFASPA 840

Query: 1520 IDGVQHRLYVASTSGRMTALLIKARPFSTLWHYDLEAPVFGSLVIDHLNRNVICCLVDGH 1579
            ID     LYVASTSGR+ A+ IK  PF TLW ++LEAP+FGSL I    +NVICCLVDG 
Sbjct: 841  IDEGHSSLYVASTSGRVIAVSIKDSPFHTLWLFELEAPIFGSLCITPSTQNVICCLVDGQ 900

Query: 1580 VVALDSSGSVSWRCKTGGPIFAGACISSAIPSQVLICSRNGSIHSFELETGNLVWEYNIG 1588
            V+A+  SG++ WR +TGGPIFAG C+S  +PSQVL+C RNG ++S E E+G LVWE NIG
Sbjct: 901  VIAMSPSGTIIWRYRTGGPIFAGPCMSHVLPSQVLVCCRNGCVYSLEPESGCLVWEDNIG 960

BLAST of Sgr025667 vs. ExPASy Swiss-Prot
Match: Q9SRQ7 (Non-specific phospholipase C4 OS=Arabidopsis thaliana OX=3702 GN=NPC4 PE=1 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 8.7e-200
Identity = 336/495 (67.88%), Postives = 388/495 (78.38%), Query Frame = 0

Query: 6   TGTGGDGKATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPN 65
           T  GG G     PIKT+VVLVQENRSFDH LGW K LN EIDGVT  +  SN +S+S+ N
Sbjct: 4   TTKGGSGS---YPIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTN 63

Query: 66  SPSVHFGNASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNL-QPAMRGFAQNAERNSKG 125
           S  V FG+ S YV+PDPGHSIQDIYEQ+FG+PW       N   P M GFAQNAERN KG
Sbjct: 64  SLRVVFGDQSQYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNAERNKKG 123

Query: 126 MSETVMNGFKPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQ 185
           MS  VMNGFKP A+ V+KELV  F +CDRWFASVPASTQPNRLY+HSATSHG +SND K 
Sbjct: 124 MSSAVMNGFKPNALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKL 183

Query: 186 LIGGLPQKTIFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREG 245
           L+ G PQKTIFESLDE GF+FGIYYQ+ P+TL YRNLRKLKY+ +FH + I FK+ C+EG
Sbjct: 184 LLEGFPQKTIFESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEG 243

Query: 246 KLPNYVVIEQRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDE 305
           KLPNYVV+EQR+FDL S P NDDHPSHDVSEGQK +KEVYEALRSSPQWNEILF+ITYDE
Sbjct: 244 KLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDE 303

Query: 306 HGGFFDHVPTPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVGPQ 365
           HGGF+DHVPTPV GVPNPDG++GPPPYNF+F+RLGVRVPT F+SPWIEPGTV+H P GP 
Sbjct: 304 HGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPY 363

Query: 366 PTSEFEHSSIAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLRDV 425
           P S++EHSSI ATVK IF+LK FL+KRD WAGTFE V+ R SPR DCP TL+ P+KLR  
Sbjct: 364 PRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTFESVITRDSPRQDCPETLSTPIKLRGT 423

Query: 426 GANDTRRISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECE 485
            A +  ++SEFQE+LV +AA LKGD K E    KL +   V++A  Y  NA + FL E  
Sbjct: 424 MAKENAQLSEFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESR 483

Query: 486 KARENGADESQIVVC 500
           KAR+ G DE+ IV C
Sbjct: 484 KARDRGCDENDIVYC 495

BLAST of Sgr025667 vs. ExPASy Swiss-Prot
Match: Q9SRQ6 (Non-specific phospholipase C3 OS=Arabidopsis thaliana OX=3702 GN=NPC3 PE=1 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 2.3e-192
Identity = 326/517 (63.06%), Postives = 389/517 (75.24%), Query Frame = 0

Query: 4   EITGTGGDGKATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSE 63
           E T +GG   A  +PIKT+VVLVQENRSFDHMLGW K LN EIDGV+     SNP+STS+
Sbjct: 3   EETSSGGGSSA--SPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSD 62

Query: 64  PNSPSVHFGNASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNAERNSK 123
           PNS  + FG  S  +DPDPGHS Q IYEQ+FG+P+S+  +S    P M GF QNAE  +K
Sbjct: 63  PNSAQIFFGKESQNIDPDPGHSFQAIYEQVFGKPFSD--ESPYPDPKMNGFVQNAEAITK 122

Query: 124 GMSE-TVMNGFKPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDT 183
           GMSE  VM GF PE + VFKELV EF VCDRWF+S+P+STQPNRLY+H+ATS+G  SNDT
Sbjct: 123 GMSEKVVMQGFPPEKLPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDT 182

Query: 184 KQLIGGLPQKTIFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCR 243
             L+ G PQ+T+FESL+E GF FGIYYQ  P  L YRN+RKLKYV NFH + + FKRHC+
Sbjct: 183 NTLVRGFPQRTVFESLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCK 242

Query: 244 EGKLPNYVVIEQRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITY 303
           EGKLPNYVVIE RYF + S P NDDHP +DV EGQ  +KE+YEALR+SPQWNEILF++ Y
Sbjct: 243 EGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVY 302

Query: 304 DEHGGFFDHVPTPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVG 363
           DEHGG++DHVPTPV+GVPNPDGLVGP PYNFKFDRLGVRVP + +SPWIEPGTV+H P G
Sbjct: 303 DEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNG 362

Query: 364 PQPTSEFEHSSIAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLR 423
           P+PTS+FEHSSI AT+KKIF LK FLTKRDEWAGT + V+NR SPRTDCPVTL +  + R
Sbjct: 363 PEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTLDAVINRTSPRTDCPVTLPELPRAR 422

Query: 424 DVG---ANDTRRISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSF 483
           D+      +   +++FQ EL+Q AAVLKGD  K++YP KL + M V +A  Y E A   F
Sbjct: 423 DIDIGTQEEDEDLTDFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRF 482

Query: 484 LHECEKARENGADESQIVVCGNQPQPSSKPKSFARSL 517
             E +KA+E G DE +IV         S PKSF + L
Sbjct: 483 HGESKKAKEEGRDEHEIVDLSKGSTRHSTPKSFVQKL 515

BLAST of Sgr025667 vs. ExPASy Swiss-Prot
Match: Q9S816 (Non-specific phospholipase C5 OS=Arabidopsis thaliana OX=3702 GN=NPC5 PE=1 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 5.6e-191
Identity = 326/505 (64.55%), Postives = 382/505 (75.64%), Query Frame = 0

Query: 18  PIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPNSPSVHFGNASGY 77
           PIKT+VVLVQENRSFDH LGW K LN EIDGV   +Q  NP  +S+ NS +V FG+ S Y
Sbjct: 12  PIKTIVVLVQENRSFDHTLGWFKELNREIDGVMKSDQKFNPGFSSDLNSHNVVFGDQSQY 71

Query: 78  VDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPA-MRGFAQNAERNSKGMSETVMNGFKPE 137
           VDP+PGHSI+DIYEQ+FG+PW       N  PA M GFAQNAER  KGMS  VMNGFKP+
Sbjct: 72  VDPNPGHSIRDIYEQVFGKPWDSGHPDPNPGPATMSGFAQNAERKMKGMSSAVMNGFKPD 131

Query: 138 AVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQLIGGLPQKTIFE 197
           A+ V+KELV  F +CDRWFASVP +TQPNRL++HSATSHG ++N+ K LI G PQKTIFE
Sbjct: 132 ALPVYKELVQNFAICDRWFASVPGATQPNRLFIHSATSHGTTNNERKLLIEGFPQKTIFE 191

Query: 198 SLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREGKLPNYVVIEQRY 257
           SLDE GF FGIYYQ  P TL YRNLRKLKY+  FH + + FK+ C+EG LPNYVV+EQR+
Sbjct: 192 SLDEAGFTFGIYYQCFPTTLFYRNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVVVEQRW 251

Query: 258 FDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPTPV 317
           +DL   P NDDHPSHDVSEGQK +KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVPTP+
Sbjct: 252 YDLLLNPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPL 311

Query: 318 VGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVGPQPTSEFEHSSIAA 377
            GVPNPDG++GPPPYNF+F+RLGVRVPT F+SPWIEPGTV+H   GP   S++EHSSI A
Sbjct: 312 DGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEHSSIPA 371

Query: 378 TVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLRDVGANDTRRISEFQ 437
           TVKKIF+LK FLTKRD WAGTFE V+ R SPR DCP TL++PVK+R   A +   +S+FQ
Sbjct: 372 TVKKIFKLKDFLTKRDSWAGTFESVITRNSPRQDCPETLSNPVKMRGTVAKENAELSDFQ 431

Query: 438 EELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECEKARENGADESQI 497
           EELV +AA LKGD K E    KL +   VS+A  Y   A   F+ E +KARE G DE+ I
Sbjct: 432 EELVIVAAGLKGDYKNEELLYKLCKKTCVSDASKYVTKAFDKFVEESKKARERGGDENDI 491

Query: 498 VVCGN--------QPQPSSKPKSFA 514
           V C +        +P PS    S A
Sbjct: 492 VFCVDDDDDHNVVKPPPSQSEPSHA 516

BLAST of Sgr025667 vs. ExPASy Swiss-Prot
Match: O81020 (Non-specific phospholipase C2 OS=Arabidopsis thaliana OX=3702 GN=NPC2 PE=2 SV=1)

HSP 1 Score: 579.3 bits (1492), Expect = 1.3e-163
Identity = 291/486 (59.88%), Postives = 362/486 (74.49%), Query Frame = 0

Query: 17  NPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPNSPSVHFGNASG 76
           +PIKT+VV+V ENRSFDHMLGWMK LN EI+GV  D   SNP+S S+P+S  + FG+ S 
Sbjct: 25  SPIKTIVVVVMENRSFDHMLGWMKKLNPEINGV--DGSESNPVSVSDPSSRKIKFGSGSH 84

Query: 77  YVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNA--ERNSKGMSETVMNGFK 136
           YVDPDPGHS Q I EQ+FG     ++ +    P M GF Q A  E  S  MS +VMNGF+
Sbjct: 85  YVDPDPGHSFQAIREQVFG-----SNDTSMDPPPMNGFVQQAYSEDPSGNMSASVMNGFE 144

Query: 137 PEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQLIGGLPQKTI 196
           P+ V V+K LV+EF V DRWFASVP+STQPNR+++HS TS G +SN+   L  G PQ+TI
Sbjct: 145 PDKVPVYKSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQRTI 204

Query: 197 FESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREGKLPNYVVIEQ 256
           F++LD+E F+FGIYYQ +PA L Y++LRKLKYV  FH +   FK H ++GKLP Y VIEQ
Sbjct: 205 FDNLDDEEFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQ 264

Query: 257 RYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPT 316
           RY D    P +DDHPSHDV +GQKFIKEVYE LR+SPQWNE L +ITYDEHGG+FDHVPT
Sbjct: 265 RYMDTLLEPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPT 324

Query: 317 PVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVG-PQPTSEFEHSS 376
           PV  VP+PDG+VGP P+ F+F+RLG+RVPT+ VSPWIE GTVVH P G P P+SE+EHSS
Sbjct: 325 PVRNVPSPDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSS 384

Query: 377 IAATVKKIFRLKQ-FLTKRDEWAGTFEIVLN-RQSPRTDCPVTLNDPVKLRDVGANDTRR 436
           I ATVKK+F L   FLTKRDEWAGTFE +L  R+ PRTDCP TL +PVK+R   AN+   
Sbjct: 385 IPATVKKLFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEKAL 444

Query: 437 ISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECEKARENGA 496
           ++EFQ+ELVQLAAVLKGD+    +P+++ + M+V E   Y E+A+K FL     A   GA
Sbjct: 445 LTEFQQELVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGA 503

Query: 497 DESQIV 498
           ++ ++V
Sbjct: 505 NKEELV 503

BLAST of Sgr025667 vs. ExPASy TrEMBL
Match: A0A5N6QL55 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_003676 PE=4 SV=1)

HSP 1 Score: 2023.4 bits (5241), Expect = 0.0e+00
Identity = 1023/1657 (61.74%), Postives = 1220/1657 (73.63%), Query Frame = 0

Query: 14   ATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPNSPSVHFGN 73
            A   PIKTVVVLVQENRSFDH+LGWMKSLN EI+GVT     SNP+ST+EP+S  +++G+
Sbjct: 8    AATYPIKTVVVLVQENRSFDHILGWMKSLNPEINGVTGKE--SNPLSTTEPSSKQIYYGD 67

Query: 74   ASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNAERNSKGMSETVMNGF 133
             S +V PDPGHSIQ IYEQ+FGEPWSE S +K L P M GFAQNAER   G+SETV+NGF
Sbjct: 68   KSVFVVPDPGHSIQAIYEQVFGEPWSEESAAKGLSPNMSGFAQNAERTETGLSETVLNGF 127

Query: 134  KPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQLIGGLPQKT 193
             P+ V VFKELV+EF VCDRWFASVPASTQPNRLY+HSATSHGLS NDTKQLI G+PQKT
Sbjct: 128  LPDNVQVFKELVSEFAVCDRWFASVPASTQPNRLYVHSATSHGLSGNDTKQLIEGMPQKT 187

Query: 194  IFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREGKLPNYVVIE 253
            IFES+ E G +FGIYYQY PATL YRNLRKLKY+ +FH F+++FK+HC EGKLPNYVVIE
Sbjct: 188  IFESVHEAGLSFGIYYQYPPATLYYRNLRKLKYLIHFHDFNLEFKKHCEEGKLPNYVVIE 247

Query: 254  QRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVP 313
            QR+FDL S+P NDDHPSHDVS GQKFIKEVYE LR+SPQWNE+LF+I YDEHGGF+DHVP
Sbjct: 248  QRWFDLLSIPANDDHPSHDVSVGQKFIKEVYETLRASPQWNEMLFIIIYDEHGGFYDHVP 307

Query: 314  TPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVGPQPTSEFEHSS 373
            TP VGVP+PD L+GP PYNFKFDRLGVRVP + +SPWIE GTV+H P GP PTSEFEHSS
Sbjct: 308  TPAVGVPSPDDLIGPAPYNFKFDRLGVRVPAILISPWIERGTVLHGPSGPYPTSEFEHSS 367

Query: 374  IAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLRDVGANDTRRIS 433
            IAATVKKIF LK FLTKRDEWAGTFE VL R SPRTDCPVTL +P KLR+ G  +  ++S
Sbjct: 368  IAATVKKIFNLKDFLTKRDEWAGTFEGVLTRTSPRTDCPVTLGEPAKLRETGPQEEAKLS 427

Query: 434  EFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECEKARENGADE 493
            EFQEELVQLAAVL GD +K++YP KLVENM+V EA  Y + A K F  EC KARE+G DE
Sbjct: 428  EFQEELVQLAAVLNGDHRKDIYPDKLVENMTVGEAAKYVQEAFKKFQDECAKARESGVDE 487

Query: 494  SQIVVCGNQPQPSSKPKSFARSLSKLYFIV-----DFSSFVAFKCTRSSSTMKQ--PPCC 553
             +IVVC       +      R  S L           SSF A     S+S  ++    CC
Sbjct: 488  DEIVVCATTASSLASKSLVHRIFSCLICDAIKRRNSISSFAAEMSDESASGERKQCSCCC 547

Query: 554  ISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGDKVISGDGADNFFKERAISAFPSM 613
            ISHEF R AS +P+KIA IHASGG Q+ +EL        +     D  FKERA S  P +
Sbjct: 548  ISHEFFRAASKNPNKIAVIHASGGAQISKELS-------VDDIDTDKLFKERAKSLSPPV 607

Query: 614  YEGDRCFTYSQLLASVDSLSSRLLPILRDA-ADDHQLITPTAPPRANDGNGEPAKTDRMT 673
            Y+GDRCFTYS +LASVDSLS+RL  IL DA ADD  LI  +        + + AK+   +
Sbjct: 608  YQGDRCFTYSDVLASVDSLSARLRSILLDAVADDPHLIAHSPKGNNTSNHAQMAKSSASS 667

Query: 674  AELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKTRILSVVS 733
                E S E +S  +PKI GIYMPPSVEYI+AVLS+LRCG AFMPLDP+WPK RILS  +
Sbjct: 668  MLRAEQSTEFKSIYVPKIVGIYMPPSVEYIVAVLSVLRCGAAFMPLDPSWPKERILSAAA 727

Query: 734  SSKIDLIIYSGSSF-CEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSANLVFPC 793
            SS +D+II   SSF    GY L D  HWL++ S CS  CF+MEE  + E    ANLV+PC
Sbjct: 728  SSNVDVIIGCASSFGMSSGYQL-DRSHWLLECSSCSVLCFSMEE-CLEECIRPANLVWPC 787

Query: 794  EHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKTSISFID 853
            + G+ RLFCY+MYTSGSTGKPKG+CGTEQGL+NRF WMQ+L+P  G+E+++FKTSISFID
Sbjct: 788  QIGEERLFCYLMYTSGSTGKPKGVCGTEQGLINRFLWMQDLYPLQGEEILMFKTSISFID 847

Query: 854  HIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLPALQRLY 913
            H+QEFL A+LT+  LVIPP  ELK+N++S+V+F+Q Y I++LT+VPSLM+A+LPALQ   
Sbjct: 848  HLQEFLGAILTACPLVIPPFSELKDNMFSVVDFLQVYFINRLTSVPSLMKAILPALQSQS 907

Query: 914  LMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCKRMPMIL 973
                  SL+LL+LSGE+LP+ LW+ L KLLPET+ILN+YGSTEVSGDCTYFDCKR+PMIL
Sbjct: 908  NRGIPTSLKLLVLSGEVLPLALWDKLAKLLPETSILNIYGSTEVSGDCTYFDCKRLPMIL 967

Query: 974  ETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLGGIFSQD 1033
            + + +  VPIG+P+S CDV++V +N   N+GE+ VGG CV  GYYSDST + L       
Sbjct: 968  DMDTLTSVPIGMPLSNCDVLLVGENGTSNQGEIYVGGVCVSCGYYSDSTVMSLDCAKLPQ 1027

Query: 1034 LVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIEDTLREHL 1093
               G S   + SQ+Y  TGDF +RLQSGDLVFLGRKDR++KVNGQRIALEEIED LR H 
Sbjct: 1028 NSVGSSSTEHGSQLYFRTGDFARRLQSGDLVFLGRKDRTVKVNGQRIALEEIEDVLRTHP 1087

Query: 1094 DVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPNSFFFIDS 1153
            DV+ AAVVS +   EL  L AF+VLK+ +  E+FRSSIRSWM++K+   M+PN F F +S
Sbjct: 1088 DVLEAAVVSSKGQWELVALEAFIVLKEERSREIFRSSIRSWMIDKLLSVMLPNHFTFTES 1147

Query: 1154 IPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEVSSDDDFF 1213
            IP+SSSGKVDYELL   T L E   + I    ++D +QV+KKAF+DALMVEEVS DDDFF
Sbjct: 1148 IPVSSSGKVDYELLAGLTSLTEPVQDKIGDMGSSDLLQVVKKAFTDALMVEEVSDDDDFF 1207

Query: 1214 MMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDA--------- 1273
            MMGGNS+ AAH+SH LGVDMR++Y++P+P+KL  ALLEK+G   + + +DA         
Sbjct: 1208 MMGGNSIAAAHLSHNLGVDMRFIYYFPSPSKLYLALLEKRGPGHLHVKKDANWEVNLDEG 1267

Query: 1274 --------------------------------------------DSRKNLKTD------- 1333
                                                        DS  N+ +D       
Sbjct: 1268 KRSTLRSINFEAPDPGIFKPQGSLLRTSVEKNDSNVIVSKRLKVDSNINVTSDSASARDG 1327

Query: 1334 --------------SRCNKVVYEHKYIGNKKCAETLSVKSQRGENGSMKKLWQVHMESCV 1393
                          SRCNKV+YE  Y GNK C  T SVK  R   GSM++ W+VHMESCV
Sbjct: 1328 YVWDSASKLMSCSASRCNKVMYEEGYSGNKICQATWSVKIPRDRKGSMQEFWKVHMESCV 1387

Query: 1394 DASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEGRIECSTAIVGDFSQVVVG 1453
            DASP+VVFK  + YLFIGSHS KF+CV AK+ S+QWE++LEGRIECS AI+GDFSQVVVG
Sbjct: 1388 DASPIVVFKDQDIYLFIGSHSCKFLCVAAKSGSVQWEIKLEGRIECSAAILGDFSQVVVG 1447

Query: 1454 CYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCGSYDHNLYALDYVRHSCVY 1513
            CYKGKIYFL+FS G I WTFQT GEVKSQPVVD    L+WCGS+DHNLYALDY  H CVY
Sbjct: 1448 CYKGKIYFLDFSNGNICWTFQTSGEVKSQPVVDIHNQLVWCGSHDHNLYALDYKNHCCVY 1507

Query: 1514 KLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFSTLWHYDLEAPVFGSLVIDH 1573
             +PCGGS+YGSPAID V + LYVASTSGRMTA+  K+ PF+ LW ++ E PVFGSL I+ 
Sbjct: 1508 MVPCGGSIYGSPAIDEVHNTLYVASTSGRMTAISTKSLPFNILWLHEFEVPVFGSLAINS 1567

Query: 1574 LNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSAIPSQVLICSRNGSIHSFE 1588
            LN NVICCLVDGHV+ALDSSGS+ W+ +TGGPIFAG CIS+A+PSQ LICSR+G I+S E
Sbjct: 1568 LNGNVICCLVDGHVLALDSSGSILWKYRTGGPIFAGPCISAALPSQGLICSRDGGIYSLE 1627

BLAST of Sgr025667 vs. ExPASy TrEMBL
Match: A0A5J4ZQQ3 (4-coumarate--CoA ligase OS=Nyssa sinensis OX=561372 GN=F0562_014605 PE=4 SV=1)

HSP 1 Score: 1899.8 bits (4920), Expect = 0.0e+00
Identity = 997/1720 (57.97%), Postives = 1197/1720 (69.59%), Query Frame = 0

Query: 1    MAPEITGTGGDGKATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPIS 60
            MA EIT        +P PIKT+VVLVQENRSFDHMLGWMKSLN EI+GVT     SNP+S
Sbjct: 1    MASEIT--------SPYPIKTIVVLVQENRSFDHMLGWMKSLNPEINGVTGTE--SNPLS 60

Query: 61   TSEPNSPSVHFGNASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNAER 120
            TS+ NS  + F + S YVDPDPGHSIQD+YEQIFG PWS+   SK LQP M GFAQNAER
Sbjct: 61   TSDRNSKRIFFRDRSAYVDPDPGHSIQDMYEQIFGMPWSQELSSKKLQPTMEGFAQNAER 120

Query: 121  NSKGMSETVMNGFKPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSN 180
               GMS+TVM GFKP+ V V+KELV+EF VCD+WFA+VPASTQPNRLY+HSATSHG +SN
Sbjct: 121  IEAGMSDTVMKGFKPDDVPVYKELVSEFAVCDQWFAAVPASTQPNRLYVHSATSHGATSN 180

Query: 181  DTKQLIGGLPQKTIFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRH 240
            DT QLI G PQKTIFES+DE G+ FGIYYQY PATL YRNLRKLKY+KNFH FD+DFKRH
Sbjct: 181  DTSQLIEGHPQKTIFESMDEAGYTFGIYYQYPPATLFYRNLRKLKYIKNFHQFDLDFKRH 240

Query: 241  CREGKLPNYVVIEQRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLI 300
            C EGKLPNYVV+EQRYFDL  LPGNDDHPSHDV EGQKF+KEVYEALR+SPQWNE+LF+I
Sbjct: 241  CEEGKLPNYVVLEQRYFDLKVLPGNDDHPSHDVFEGQKFVKEVYEALRASPQWNEMLFII 300

Query: 301  TYDEHGGFFDHVPTPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRP 360
             YDEHGGF+DHVPTPV GVP+PDG++GP PYNF+FDRLGVRVP + +SPWIEPGTV+HRP
Sbjct: 301  IYDEHGGFYDHVPTPVTGVPSPDGIMGPEPYNFQFDRLGVRVPALMISPWIEPGTVLHRP 360

Query: 361  VGPQPTSEFEHSSIAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVK 420
             GP P+SEFEHSS+ ATVKKIF L +FLT+RD WAGTFE+VLNR+SPRTDCPVTL +PVK
Sbjct: 361  SGPYPSSEFEHSSVPATVKKIFNLNEFLTRRDAWAGTFEVVLNRKSPRTDCPVTLPEPVK 420

Query: 421  LRDVGANDTRRISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFL 480
            LR+  A +  ++SEFQEELVQ+AA L GD +K++YP +LVENM+VSEAV Y  NA K FL
Sbjct: 421  LREAEAEENGKLSEFQEELVQMAATLCGDHRKDIYPHRLVENMTVSEAVEYVNNAFKKFL 480

Query: 481  HECEKARENGADESQIVVCGNQPQP-------------------SSKPKSFARSLS---- 540
             ECE AR +GADES I V  +QP+P                   S++PK  A S+     
Sbjct: 481  DECESARASGADESDICVPRDQPKPAESKSVASKLFSCLAEPILSTRPKIPAHSVQLKRR 540

Query: 541  ---------KLYFIVDFSSFVAFKCTRSSSTMK---------------QPPCCISHEFQR 600
                     K  F      F  ++ T S S +                   CCISHEF +
Sbjct: 541  RSNSVSDHLKRKFASGGRIFATYQRTGSPSFLAGKRSSFSLCSLQLRWLAGCCISHEFFK 600

Query: 601  VASAHPDKIAAIHASGGVQL---FRELHGGGGDKV-ISGDGADNFFK--ERAISAFPSMY 660
             AS +P K+A IHA GG  +   FR  H  G D + IS    DNF        S+   +Y
Sbjct: 601  AASKNPSKVAVIHACGGANIAREFRNHHTIGNDNITISEIDYDNFVNGLNTESSSHSPVY 660

Query: 661  EGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEPA----KTDR 720
            EGDRCFT+S++LASVDSLSSRL  IL   +D H +     P   N  + +P         
Sbjct: 661  EGDRCFTFSEILASVDSLSSRLRHILDGGSDPHLI----KPATGNFPSEQPVDVHISESN 720

Query: 721  MTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKTRILSV 780
             ++   E S E +    PKI GIYM PSVEY+IAVLS+LRCG AFMPLDP WPK RILSV
Sbjct: 721  SSSPGVEQSTEYQHMYTPKILGIYMVPSVEYVIAVLSVLRCGEAFMPLDPLWPKERILSV 780

Query: 781  VSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSANLVFP 840
            VSSS +DLII   SSF     H  D  HWLV  S C     +M+ N ++E   S+ LV+P
Sbjct: 781  VSSSNVDLIIGCQSSFDGSWCHELDKSHWLVDCSSCPVLFISMKAN-LQEKFGSSYLVWP 840

Query: 841  CEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKTSISFI 900
            CE G+ R FCY+MYTSGSTGKPKG+CGTE GLLNRF WMQEL P  G+E++ FKTSISFI
Sbjct: 841  CEKGRLRSFCYLMYTSGSTGKPKGVCGTEPGLLNRFMWMQELHPLLGEEILFFKTSISFI 900

Query: 901  DHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLPALQRL 960
            DH+QEF+ A+LT+  LVIPP  EL+ENL+ +++F+QAYSIS+L AVPSLMRAVLPALQ+ 
Sbjct: 901  DHLQEFVGALLTTCTLVIPPFNELRENLFYMIDFLQAYSISRLIAVPSLMRAVLPALQKP 960

Query: 961  YLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCKRMPMI 1020
            Y  + + SL+LL+LSGE+LP+ LW+ L+KLLP+TTILNLYGSTEVSGDCTYFDCKR+PMI
Sbjct: 961  YNTRIQSSLKLLVLSGEVLPLSLWDMLYKLLPKTTILNLYGSTEVSGDCTYFDCKRLPMI 1020

Query: 1021 LETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLGGI-FS 1080
            LE+E ++ VPIG+PIS CDVV+V + ++ N+GE+ VGG CV +GY  D   +    +   
Sbjct: 1021 LESEDLSSVPIGMPISNCDVVLVGE-ESPNQGEIYVGGICVAAGYLCDPYIMQQDFVKLP 1080

Query: 1081 QDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIEDTLRE 1140
            QD     S + +  Q Y +TGDF +RLQSGD VF+GRKDR++KVNGQRIALEEIE TLR 
Sbjct: 1081 QDFCCDCSISEHGRQNYFNTGDFARRLQSGDFVFIGRKDRTVKVNGQRIALEEIESTLRG 1140

Query: 1141 HLDVVNAAVVSGRSDRELEYLVAFLVLKDNKK-SEVFRSSIRSWMVEKVPLAMIPNSFFF 1200
            H DVV+AAVVS + + E+  + A+LV+K   +  E+ RSSIR                  
Sbjct: 1141 HPDVVDAAVVSHKDEGEVMLVDAYLVIKQKDECGEILRSSIR------------------ 1200

Query: 1201 IDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEVSSDD 1260
                     GKVDY LL   T         ID   +  F+Q IKKAF DALMVE VS+DD
Sbjct: 1201 ---------GKVDYSLLASLTFSMTHVQNEIDEIQSTSFLQDIKKAFCDALMVEMVSNDD 1260

Query: 1261 DFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIID--ISRDADSRK 1320
            DFF MGGNS++AAHVSH LG+DMR LY +P+P  L  ALL+K G   +D  +  DA+   
Sbjct: 1261 DFFAMGGNSISAAHVSHNLGIDMRLLYIFPSPLMLQLALLQKIGLCNVDVRVRTDANWGV 1320

Query: 1321 NLKTD------------------------------------------------------- 1380
            NLKT                                                        
Sbjct: 1321 NLKTHVDSTLLSFDSKTPNLYSSKSRGRFSSTLHEKNDNYPVKCLKVDSKLHLNSKGIGH 1380

Query: 1381 -----------------SRCNKVVYEHKYIGNKKCAETLSVKSQRGENGSMKKLWQVHME 1440
                             SRCNKV+YE +  GN     T S +  R + G+M +LW+VH+ 
Sbjct: 1381 GDGYPWYSNSIHMACSFSRCNKVIYEGENAGNSLFQATWSAEIPRDKKGAMLELWKVHLG 1440

Query: 1441 SCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEGRIECSTAIVGDFSQV 1500
            SCVDASP+VVFK  +T+LFIGSHS KF+C++AK+  +QWE++LEGRIECS AI+GDFSQV
Sbjct: 1441 SCVDASPMVVFKDQDTFLFIGSHSHKFLCINAKSGFVQWEIKLEGRIECSAAILGDFSQV 1500

Query: 1501 VVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCGSYDHNLYALDYVRHS 1560
            VVGCY+G IYFL+F  G I WTFQTCGEVKSQP+VD  R+L+WCGSYDHNLYALDY  + 
Sbjct: 1501 VVGCYQGNIYFLDFLDGKIHWTFQTCGEVKSQPLVDKCRSLVWCGSYDHNLYALDYKNYC 1560

Query: 1561 CVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFSTLWHYDLEAPVFGSLV 1588
            CVYKLPCGGS+YGSPAID V   LYVASTSGR+TA+ IKA PFS LW  +LE PVFGSL 
Sbjct: 1561 CVYKLPCGGSIYGSPAIDEVHDTLYVASTSGRITAIYIKALPFSKLWLRELETPVFGSLS 1620

BLAST of Sgr025667 vs. ExPASy TrEMBL
Match: A0A6J1DN92 (putative acyl-activating enzyme 19 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111022786 PE=4 SV=1)

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 900/1135 (79.30%), Postives = 947/1135 (83.44%), Query Frame = 0

Query: 540  MKQPPCCISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGD-KVISGDGADNFFKER 599
            MKQPPCCI HEFQRV+SAHP KIA IHASGGVQLFR+LHGGGGD  +ISGDGADNFFKER
Sbjct: 1    MKQPPCCIFHEFQRVSSAHPHKIAVIHASGGVQLFRQLHGGGGDSNIISGDGADNFFKER 60

Query: 600  AISAFPSMYEGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEP 659
            AISAFPSMYEGDR FTYS LLASVDSLSSRLL                   RANDGNG  
Sbjct: 61   AISAFPSMYEGDRFFTYSHLLASVDSLSSRLL------------------IRANDGNGFS 120

Query: 660  AKTDRMTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKT 719
                        +S  LE +NIPKIFGIYMPPSVEYI+AVLS+LRCGGAFMPLDPAWPK+
Sbjct: 121  ----------EGSSTGLEGANIPKIFGIYMPPSVEYIVAVLSVLRCGGAFMPLDPAWPKS 180

Query: 720  RILSVVSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSA 779
            RILSVVSSSK++LIIYSGSSFCEDGYHLSDGLHWL+QSSGC TFCF MEE+ I+EHNSS 
Sbjct: 181  RILSVVSSSKVELIIYSGSSFCEDGYHLSDGLHWLLQSSGCPTFCFNMEESFIQEHNSSV 240

Query: 780  NLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKT 839
            +LVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFP SG+EL+LFKT
Sbjct: 241  DLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPCSGEELLLFKT 300

Query: 840  SISFIDHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLP 899
             ISFIDHIQEFLSA+LTSSALVIPPMKELKE L S+VNFIQAYSISKLTAVPSLMRAVLP
Sbjct: 301  PISFIDHIQEFLSAILTSSALVIPPMKELKETLCSVVNFIQAYSISKLTAVPSLMRAVLP 360

Query: 900  ALQRLYLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCK 959
            A QRLY+MQNRCSLRLLILSGEIL IQLW AL KLLPETTILNLYGSTEVSGDCTYFDCK
Sbjct: 361  AFQRLYVMQNRCSLRLLILSGEILSIQLWKALLKLLPETTILNLYGSTEVSGDCTYFDCK 420

Query: 960  RMPMILETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLG 1019
            RMP ILETEAIN VPIGVPIS CDVVVV +NDA N+GELCVGGPCVCSGYYSDSTFLPL 
Sbjct: 421  RMPRILETEAINTVPIGVPISHCDVVVVGENDAPNQGELCVGGPCVCSGYYSDSTFLPLD 480

Query: 1020 GI-FSQDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 1079
            G   SQ LV+GGS N NC +IYI TGDFV+RLQSGDLVFLGRKDRSIKVNGQRIALEEIE
Sbjct: 481  GTKLSQGLVNGGSLNENC-KIYIRTGDFVRRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 540

Query: 1080 DTLREHLDVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPN 1139
            D L EH DVVNAA VS RSDRELEYLVAFLVLKDNKKSEVF+ S+RSWMV+KVPLAMIPN
Sbjct: 541  DALMEHPDVVNAAAVSSRSDRELEYLVAFLVLKDNKKSEVFK-SVRSWMVDKVPLAMIPN 600

Query: 1140 SFFFIDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEV 1199
             F  +DSIPMSSSGKVDYEL+MHS PLWE  HEN D T  NDFMQVIKK FSD LMVEEV
Sbjct: 601  RFICVDSIPMSSSGKVDYELVMHSYPLWEHVHENFDETQENDFMQVIKKVFSDVLMVEEV 660

Query: 1200 SSDDDFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDADS 1259
            SS+DDFFMMGGNS+TAAHVSHKLGVD+RWLYHYP+PAKLLTALLEKKGSDIIDISRD DS
Sbjct: 661  SSNDDFFMMGGNSITAAHVSHKLGVDIRWLYHYPSPAKLLTALLEKKGSDIIDISRDVDS 720

Query: 1260 RKNLKTD----------------------------------------------------- 1319
            RKNL+TD                                                     
Sbjct: 721  RKNLRTDKWNKFSFEGSEILNPFDLKEGGNFGKRKQVQSNETLSRVAIPRNDNSSISKHY 780

Query: 1320 --------------------------------SRCNKVVYEHKYIGNKKCAETLSVKSQR 1379
                                            SRCNKVVYEHKYIGN +CAETLSVKSQR
Sbjct: 781  KAVSDFSVNLEHISQVGGHLWNSLLTSMSCAFSRCNKVVYEHKYIGNNECAETLSVKSQR 840

Query: 1380 GENGSMKKLWQVHMESCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEG 1439
            GE GSMKK WQVHMESCVDASPL+VFKHP  YLFIGSHSQKFVCVDAK ASLQWE+RLEG
Sbjct: 841  GEYGSMKKFWQVHMESCVDASPLLVFKHPCIYLFIGSHSQKFVCVDAKTASLQWEIRLEG 900

Query: 1440 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCG 1499
            RIECSTAIVGDFSQVVVGCY+GKIYFLEFSTGII WTFQTCGEVKSQPVVDS RNLIWCG
Sbjct: 901  RIECSTAIVGDFSQVVVGCYEGKIYFLEFSTGIIHWTFQTCGEVKSQPVVDSQRNLIWCG 960

Query: 1500 SYDHNLYALDYVRHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFST 1559
            SYDHNLYALDYVRH+CVYKLPCGGS+YGSPAIDGVQHRLYVASTSGR++ALLIKA PF T
Sbjct: 961  SYDHNLYALDYVRHTCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRISALLIKAFPFGT 1020

Query: 1560 LWHYDLEAPVFGSLVIDHLNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSA 1588
             WHYDLEAPVFGSLVID LNRNVICCLV+GHVVALDSSGSV WRCKTGGPIFAGACISSA
Sbjct: 1021 FWHYDLEAPVFGSLVIDPLNRNVICCLVNGHVVALDSSGSVLWRCKTGGPIFAGACISSA 1080

BLAST of Sgr025667 vs. ExPASy TrEMBL
Match: A0A6J1DNQ7 (putative acyl-activating enzyme 19 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022786 PE=4 SV=1)

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 900/1135 (79.30%), Postives = 947/1135 (83.44%), Query Frame = 0

Query: 540  MKQPPCCISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGD-KVISGDGADNFFKER 599
            MKQPPCCI HEFQRV+SAHP KIA IHASGGVQLFR+LHGGGGD  +ISGDGADNFFKER
Sbjct: 1    MKQPPCCIFHEFQRVSSAHPHKIAVIHASGGVQLFRQLHGGGGDSNIISGDGADNFFKER 60

Query: 600  AISAFPSMYEGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEP 659
            AISAFPSMYEGDR FTYS LLASVDSLSSRLL                   RANDGNG  
Sbjct: 61   AISAFPSMYEGDRFFTYSHLLASVDSLSSRLL------------------IRANDGNGFS 120

Query: 660  AKTDRMTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKT 719
                        +S  LE +NIPKIFGIYMPPSVEYI+AVLS+LRCGGAFMPLDPAWPK+
Sbjct: 121  ----------EGSSTGLEGANIPKIFGIYMPPSVEYIVAVLSVLRCGGAFMPLDPAWPKS 180

Query: 720  RILSVVSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSA 779
            RILSVVSSSK++LIIYSGSSFCEDGYHLSDGLHWL+QSSGC TFCF MEE+ I+EHNSS 
Sbjct: 181  RILSVVSSSKVELIIYSGSSFCEDGYHLSDGLHWLLQSSGCPTFCFNMEESFIQEHNSSV 240

Query: 780  NLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKT 839
            +LVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFP SG+EL+LFKT
Sbjct: 241  DLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPCSGEELLLFKT 300

Query: 840  SISFIDHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLP 899
             ISFIDHIQEFLSA+LTSSALVIPPMKELKE L S+VNFIQAYSISKLTAVPSLMRAVLP
Sbjct: 301  PISFIDHIQEFLSAILTSSALVIPPMKELKETLCSVVNFIQAYSISKLTAVPSLMRAVLP 360

Query: 900  ALQRLYLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCK 959
            A QRLY+MQNRCSLRLLILSGEIL IQLW AL KLLPETTILNLYGSTEVSGDCTYFDCK
Sbjct: 361  AFQRLYVMQNRCSLRLLILSGEILSIQLWKALLKLLPETTILNLYGSTEVSGDCTYFDCK 420

Query: 960  RMPMILETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLG 1019
            RMP ILETEAIN VPIGVPIS CDVVVV +NDA N+GELCVGGPCVCSGYYSDSTFLPL 
Sbjct: 421  RMPRILETEAINTVPIGVPISHCDVVVVGENDAPNQGELCVGGPCVCSGYYSDSTFLPLD 480

Query: 1020 GI-FSQDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 1079
            G   SQ LV+GGS N NC +IYI TGDFV+RLQSGDLVFLGRKDRSIKVNGQRIALEEIE
Sbjct: 481  GTKLSQGLVNGGSLNENC-KIYIRTGDFVRRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 540

Query: 1080 DTLREHLDVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPN 1139
            D L EH DVVNAA VS RSDRELEYLVAFLVLKDNKKSEVF+ S+RSWMV+KVPLAMIPN
Sbjct: 541  DALMEHPDVVNAAAVSSRSDRELEYLVAFLVLKDNKKSEVFK-SVRSWMVDKVPLAMIPN 600

Query: 1140 SFFFIDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEV 1199
             F  +DSIPMSSSGKVDYEL+MHS PLWE  HEN D T  NDFMQVIKK FSD LMVEEV
Sbjct: 601  RFICVDSIPMSSSGKVDYELVMHSYPLWEHVHENFDETQENDFMQVIKKVFSDVLMVEEV 660

Query: 1200 SSDDDFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDADS 1259
            SS+DDFFMMGGNS+TAAHVSHKLGVD+RWLYHYP+PAKLLTALLEKKGSDIIDISRD DS
Sbjct: 661  SSNDDFFMMGGNSITAAHVSHKLGVDIRWLYHYPSPAKLLTALLEKKGSDIIDISRDVDS 720

Query: 1260 RKNLKTD----------------------------------------------------- 1319
            RKNL+TD                                                     
Sbjct: 721  RKNLRTDKWNKFSFEGSEILNPFDLKEGGNFGKRKQVQSNETLSRVAIPRNDNSSISKHY 780

Query: 1320 --------------------------------SRCNKVVYEHKYIGNKKCAETLSVKSQR 1379
                                            SRCNKVVYEHKYIGN +CAETLSVKSQR
Sbjct: 781  KAVSDFSVNLEHISQVGGHLWNSLLTSMSCAFSRCNKVVYEHKYIGNNECAETLSVKSQR 840

Query: 1380 GENGSMKKLWQVHMESCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEG 1439
            GE GSMKK WQVHMESCVDASPL+VFKHP  YLFIGSHSQKFVCVDAK ASLQWE+RLEG
Sbjct: 841  GEYGSMKKFWQVHMESCVDASPLLVFKHPCIYLFIGSHSQKFVCVDAKTASLQWEIRLEG 900

Query: 1440 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCG 1499
            RIECSTAIVGDFSQVVVGCY+GKIYFLEFSTGII WTFQTCGEVKSQPVVDS RNLIWCG
Sbjct: 901  RIECSTAIVGDFSQVVVGCYEGKIYFLEFSTGIIHWTFQTCGEVKSQPVVDSQRNLIWCG 960

Query: 1500 SYDHNLYALDYVRHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFST 1559
            SYDHNLYALDYVRH+CVYKLPCGGS+YGSPAIDGVQHRLYVASTSGR++ALLIKA PF T
Sbjct: 961  SYDHNLYALDYVRHTCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRISALLIKAFPFGT 1020

Query: 1560 LWHYDLEAPVFGSLVIDHLNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSA 1588
             WHYDLEAPVFGSLVID LNRNVICCLV+GHVVALDSSGSV WRCKTGGPIFAGACISSA
Sbjct: 1021 FWHYDLEAPVFGSLVIDPLNRNVICCLVNGHVVALDSSGSVLWRCKTGGPIFAGACISSA 1080

BLAST of Sgr025667 vs. ExPASy TrEMBL
Match: A0A6J1DQ55 (putative acyl-activating enzyme 19 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022786 PE=4 SV=1)

HSP 1 Score: 1772.3 bits (4589), Expect = 0.0e+00
Identity = 900/1135 (79.30%), Postives = 947/1135 (83.44%), Query Frame = 0

Query: 540  MKQPPCCISHEFQRVASAHPDKIAAIHASGGVQLFRELHGGGGD-KVISGDGADNFFKER 599
            MKQPPCCI HEFQRV+SAHP KIA IHASGGVQLFR+LHGGGGD  +ISGDGADNFFKER
Sbjct: 1    MKQPPCCIFHEFQRVSSAHPHKIAVIHASGGVQLFRQLHGGGGDSNIISGDGADNFFKER 60

Query: 600  AISAFPSMYEGDRCFTYSQLLASVDSLSSRLLPILRDAADDHQLITPTAPPRANDGNGEP 659
            AISAFPSMYEGDR FTYS LLASVDSLSSRLL                   RANDGNG  
Sbjct: 61   AISAFPSMYEGDRFFTYSHLLASVDSLSSRLL------------------IRANDGNGFS 120

Query: 660  AKTDRMTAELTEASIELESSNIPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKT 719
                        +S  LE +NIPKIFGIYMPPSVEYI+AVLS+LRCGGAFMPLDPAWPK+
Sbjct: 121  ----------EGSSTGLEGANIPKIFGIYMPPSVEYIVAVLSVLRCGGAFMPLDPAWPKS 180

Query: 720  RILSVVSSSKIDLIIYSGSSFCEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSA 779
            RILSVVSSSK++LIIYSGSSFCEDGYHLSDGLHWL+QSSGC TFCF MEE+ I+EHNSS 
Sbjct: 181  RILSVVSSSKVELIIYSGSSFCEDGYHLSDGLHWLLQSSGCPTFCFNMEESFIQEHNSSV 240

Query: 780  NLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKT 839
            +LVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFP SG+EL+LFKT
Sbjct: 241  DLVFPCEHGKGRLFCYVMYTSGSTGKPKGICGTEQGLLNRFQWMQELFPCSGEELLLFKT 300

Query: 840  SISFIDHIQEFLSAMLTSSALVIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLP 899
             ISFIDHIQEFLSA+LTSSALVIPPMKELKE L S+VNFIQAYSISKLTAVPSLMRAVLP
Sbjct: 301  PISFIDHIQEFLSAILTSSALVIPPMKELKETLCSVVNFIQAYSISKLTAVPSLMRAVLP 360

Query: 900  ALQRLYLMQNRCSLRLLILSGEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCK 959
            A QRLY+MQNRCSLRLLILSGEIL IQLW AL KLLPETTILNLYGSTEVSGDCTYFDCK
Sbjct: 361  AFQRLYVMQNRCSLRLLILSGEILSIQLWKALLKLLPETTILNLYGSTEVSGDCTYFDCK 420

Query: 960  RMPMILETEAINIVPIGVPISQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLG 1019
            RMP ILETEAIN VPIGVPIS CDVVVV +NDA N+GELCVGGPCVCSGYYSDSTFLPL 
Sbjct: 421  RMPRILETEAINTVPIGVPISHCDVVVVGENDAPNQGELCVGGPCVCSGYYSDSTFLPLD 480

Query: 1020 GI-FSQDLVHGGSFNANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 1079
            G   SQ LV+GGS N NC +IYI TGDFV+RLQSGDLVFLGRKDRSIKVNGQRIALEEIE
Sbjct: 481  GTKLSQGLVNGGSLNENC-KIYIRTGDFVRRLQSGDLVFLGRKDRSIKVNGQRIALEEIE 540

Query: 1080 DTLREHLDVVNAAVVSGRSDRELEYLVAFLVLKDNKKSEVFRSSIRSWMVEKVPLAMIPN 1139
            D L EH DVVNAA VS RSDRELEYLVAFLVLKDNKKSEVF+ S+RSWMV+KVPLAMIPN
Sbjct: 541  DALMEHPDVVNAAAVSSRSDRELEYLVAFLVLKDNKKSEVFK-SVRSWMVDKVPLAMIPN 600

Query: 1140 SFFFIDSIPMSSSGKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEV 1199
             F  +DSIPMSSSGKVDYEL+MHS PLWE  HEN D T  NDFMQVIKK FSD LMVEEV
Sbjct: 601  RFICVDSIPMSSSGKVDYELVMHSYPLWEHVHENFDETQENDFMQVIKKVFSDVLMVEEV 660

Query: 1200 SSDDDFFMMGGNSLTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDADS 1259
            SS+DDFFMMGGNS+TAAHVSHKLGVD+RWLYHYP+PAKLLTALLEKKGSDIIDISRD DS
Sbjct: 661  SSNDDFFMMGGNSITAAHVSHKLGVDIRWLYHYPSPAKLLTALLEKKGSDIIDISRDVDS 720

Query: 1260 RKNLKTD----------------------------------------------------- 1319
            RKNL+TD                                                     
Sbjct: 721  RKNLRTDKWNKFSFEGSEILNPFDLKEGGNFGKRKQVQSNETLSRVAIPRNDNSSISKHY 780

Query: 1320 --------------------------------SRCNKVVYEHKYIGNKKCAETLSVKSQR 1379
                                            SRCNKVVYEHKYIGN +CAETLSVKSQR
Sbjct: 781  KAVSDFSVNLEHISQVGGHLWNSLLTSMSCAFSRCNKVVYEHKYIGNNECAETLSVKSQR 840

Query: 1380 GENGSMKKLWQVHMESCVDASPLVVFKHPNTYLFIGSHSQKFVCVDAKNASLQWEMRLEG 1439
            GE GSMKK WQVHMESCVDASPL+VFKHP  YLFIGSHSQKFVCVDAK ASLQWE+RLEG
Sbjct: 841  GEYGSMKKFWQVHMESCVDASPLLVFKHPCIYLFIGSHSQKFVCVDAKTASLQWEIRLEG 900

Query: 1440 RIECSTAIVGDFSQVVVGCYKGKIYFLEFSTGIIQWTFQTCGEVKSQPVVDSDRNLIWCG 1499
            RIECSTAIVGDFSQVVVGCY+GKIYFLEFSTGII WTFQTCGEVKSQPVVDS RNLIWCG
Sbjct: 901  RIECSTAIVGDFSQVVVGCYEGKIYFLEFSTGIIHWTFQTCGEVKSQPVVDSQRNLIWCG 960

Query: 1500 SYDHNLYALDYVRHSCVYKLPCGGSLYGSPAIDGVQHRLYVASTSGRMTALLIKARPFST 1559
            SYDHNLYALDYVRH+CVYKLPCGGS+YGSPAIDGVQHRLYVASTSGR++ALLIKA PF T
Sbjct: 961  SYDHNLYALDYVRHTCVYKLPCGGSIYGSPAIDGVQHRLYVASTSGRISALLIKAFPFGT 1020

Query: 1560 LWHYDLEAPVFGSLVIDHLNRNVICCLVDGHVVALDSSGSVSWRCKTGGPIFAGACISSA 1588
             WHYDLEAPVFGSLVID LNRNVICCLV+GHVVALDSSGSV WRCKTGGPIFAGACISSA
Sbjct: 1021 FWHYDLEAPVFGSLVIDPLNRNVICCLVNGHVVALDSSGSVLWRCKTGGPIFAGACISSA 1080

BLAST of Sgr025667 vs. TAIR 10
Match: AT5G35930.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 958.7 bits (2477), Expect = 5.6e-279
Identity = 497/984 (50.51%), Postives = 644/984 (65.45%), Query Frame = 0

Query: 680  IPKIFGIYMPPSVEYIIAVLSILRCGGAFMPLDPAWPKTRILSVVSSSKIDLIIYSGSSF 739
            +PK+  +YMPPSVEY+I+V S+LRCG AF+PLDP+WP+ R+LS++SSS I L+I  G S 
Sbjct: 1    MPKVVALYMPPSVEYVISVFSVLRCGEAFLPLDPSWPRERVLSLISSSNISLVIACGLSS 60

Query: 740  CEDGYHLSDGLHWLVQSSGCSTFCFTMEENPIREHNSSANLVFPCEHGKGRLFCYVMYTS 799
             E         HWLV+ + C    F+M+E  +      ++ V+PC+  + R FCY+MYTS
Sbjct: 61   VES--------HWLVERNVCPVLLFSMDEK-LSVETGCSSFVWPCKKERQRKFCYLMYTS 120

Query: 800  GSTGKPKGICGTEQGLLNRFQWMQELFPSSGDELILFKTSISFIDHIQEFLSAMLTSSAL 859
            GSTGKPKG+CGTEQGLLNRF WMQEL+P  G++   FKTS+ FIDHIQEFL A+L+S+AL
Sbjct: 121  GSTGKPKGVCGTEQGLLNRFLWMQELYPVVGEQRFAFKTSVGFIDHIQEFLGAILSSTAL 180

Query: 860  VIPPMKELKENLYSIVNFIQAYSISKLTAVPSLMRAVLPALQ-RLYLMQNRCSLRLLILS 919
            VIPP   LKEN+ SI++F++ YSIS+L AVPS++RA+LP LQ R +  + +  L+L++LS
Sbjct: 181  VIPPFTLLKENMISIIDFLEEYSISRLLAVPSMIRAILPTLQHRGHNNKLQSCLKLVVLS 240

Query: 920  GEILPIQLWNALFKLLPETTILNLYGSTEVSGDCTYFDCKRMPMILETEAINIVPIGVPI 979
            GE  P+ LW++L  LLPET  LNLYGSTEVSGDCTYFDC  +P +L+TE I  VPIG  I
Sbjct: 241  GEPFPVSLWDSLHSLLPETCFLNLYGSTEVSGDCTYFDCSELPRLLKTEEIGSVPIGKSI 300

Query: 980  SQCDVVVVDDNDALNEGELCVGGPCVCSGYYSDSTFLPLGGIFSQDLV--HGGSF----- 1039
            S C VV++ D D   EGE+CV G C+  GY   S       I S+  V  H  S      
Sbjct: 301  SNCKVVLLGDEDKPYEGEICVSGLCLSQGYMHSS-------IESEGYVKLHNNSLCNHLT 360

Query: 1040 NANCSQIYISTGDFVQRLQSGDLVFLGRKDRSIKVNGQRIALEEIEDTLREHLDVVNAAV 1099
            N   SQ+Y  TGD+ ++L SGDL+F+GR+DR++K+NG+R+ALEEIE TL  + D+  A V
Sbjct: 361  NDCGSQLYYRTGDYGRQLSSGDLIFIGRRDRTVKLNGKRMALEEIETTLELNPDIAEAVV 420

Query: 1100 VSGRSDRELEYLVAFLVL-KDNKKSEVFRSSIRSWMVEKVPLAMIPNSFFFIDSIPMSSS 1159
            +  R + EL  L AF+VL K++  S+    SIR+WM  K+P  MIPN F  ++ +P++SS
Sbjct: 421  LLSRDETELASLKAFVVLNKESNSSDGIIFSIRNWMGGKLPPVMIPNHFVLVEKLPLTSS 480

Query: 1160 GKVDYELLMHSTPLWERTHENIDGTWANDFMQVIKKAFSDALMVEEVSSDDDFFMMGGNS 1219
            GKVDYE L           + +     N  +Q IKKA  DAL+V+EVS DDDFF +GG+S
Sbjct: 481  GKVDYEALARLKCPTTGAQDMMQSNGTNSLLQNIKKAVCDALLVKEVSDDDDFFAIGGDS 540

Query: 1220 LTAAHVSHKLGVDMRWLYHYPTPAKLLTALLEKKGSDIIDISRDA--------------- 1279
            L AAH+SH LG+DMR +Y + +P++LL  L EK+G    D+  +                
Sbjct: 541  LAAAHLSHSLGIDMRLIYQFRSPSRLLIYLSEKEGKLREDMQHNTTQKLDHKIESQNGNG 600

Query: 1280 --------------------------DSRKNLKTD------------------------- 1339
                                      +S K LK D                         
Sbjct: 601  LVSRTVPLHSGVTSGPTPSKLQCEKNNSPKRLKIDYEKFSPKRMKENKLWDSGFSQIQCA 660

Query: 1340 -SRCNKVVYEHKYIGNKKCAETLSVKSQRGENGSMKKLWQVHMESCVDASPLVVFKHPNT 1399
             SRCNKV         +   E  S++  R +  SM+++W+VHMESCVDASPLVV K   T
Sbjct: 661  FSRCNKVHSPESCSNEEANREYWSLEIPRNQMVSMQEIWKVHMESCVDASPLVVLKDSKT 720

Query: 1400 YLFIGSHSQKFVCVDAKNASLQWEMRLEGRIECSTAIVGDFSQVVVGCYKGKIYFLEFST 1459
            YLFIGSHS+KF C+DAK+ S+ WE  LEGRIE S  +VGDFSQVV+GCYKGK+YFL+FST
Sbjct: 721  YLFIGSHSRKFSCIDAKSGSMYWETILEGRIEGSAMVVGDFSQVVIGCYKGKLYFLDFST 780

Query: 1460 GIIQWTFQTCGEVKSQPVVDSDRNLIWCGSYDHNLYALDYVRHSCVYKLPCGGSLYGSPA 1519
            G + W FQ CGE+K QPVVD+   LIWCGS+DH LYALDY    CVYKL CGGS++ SPA
Sbjct: 781  GSLCWKFQACGEIKCQPVVDTSSQLIWCGSHDHTLYALDYRSQCCVYKLQCGGSIFASPA 840

Query: 1520 IDGVQHRLYVASTSGRMTALLIKARPFSTLWHYDLEAPVFGSLVIDHLNRNVICCLVDGH 1579
            ID     LYVASTSGR+ A+ IK  PF TLW ++LEAP+FGSL I    +NVICCLVDG 
Sbjct: 841  IDEGHSSLYVASTSGRVIAVSIKDSPFHTLWLFELEAPIFGSLCITPSTQNVICCLVDGQ 900

Query: 1580 VVALDSSGSVSWRCKTGGPIFAGACISSAIPSQVLICSRNGSIHSFELETGNLVWEYNIG 1588
            V+A+  SG++ WR +TGGPIFAG C+S  +PSQVL+C RNG ++S E E+G LVWE NIG
Sbjct: 901  VIAMSPSGTIIWRYRTGGPIFAGPCMSHVLPSQVLVCCRNGCVYSLEPESGCLVWEDNIG 960

BLAST of Sgr025667 vs. TAIR 10
Match: AT3G03530.1 (non-specific phospholipase C4 )

HSP 1 Score: 699.5 bits (1804), Expect = 6.1e-201
Identity = 336/495 (67.88%), Postives = 388/495 (78.38%), Query Frame = 0

Query: 6   TGTGGDGKATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPN 65
           T  GG G     PIKT+VVLVQENRSFDH LGW K LN EIDGVT  +  SN +S+S+ N
Sbjct: 4   TTKGGSGS---YPIKTIVVLVQENRSFDHTLGWFKELNREIDGVTKSDPKSNTVSSSDTN 63

Query: 66  SPSVHFGNASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNL-QPAMRGFAQNAERNSKG 125
           S  V FG+ S YV+PDPGHSIQDIYEQ+FG+PW       N   P M GFAQNAERN KG
Sbjct: 64  SLRVVFGDQSQYVNPDPGHSIQDIYEQVFGKPWDSGKPDPNPGHPNMSGFAQNAERNKKG 123

Query: 126 MSETVMNGFKPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQ 185
           MS  VMNGFKP A+ V+KELV  F +CDRWFASVPASTQPNRLY+HSATSHG +SND K 
Sbjct: 124 MSSAVMNGFKPNALPVYKELVQNFAICDRWFASVPASTQPNRLYVHSATSHGATSNDKKL 183

Query: 186 LIGGLPQKTIFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREG 245
           L+ G PQKTIFESLDE GF+FGIYYQ+ P+TL YRNLRKLKY+ +FH + I FK+ C+EG
Sbjct: 184 LLEGFPQKTIFESLDEAGFSFGIYYQFPPSTLFYRNLRKLKYLTHFHQYGIQFKKDCKEG 243

Query: 246 KLPNYVVIEQRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDE 305
           KLPNYVV+EQR+FDL S P NDDHPSHDVSEGQK +KEVYEALRSSPQWNEILF+ITYDE
Sbjct: 244 KLPNYVVVEQRWFDLLSTPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDE 303

Query: 306 HGGFFDHVPTPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVGPQ 365
           HGGF+DHVPTPV GVPNPDG++GPPPYNF+F+RLGVRVPT F+SPWIEPGTV+H P GP 
Sbjct: 304 HGGFYDHVPTPVDGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVIHGPNGPY 363

Query: 366 PTSEFEHSSIAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLRDV 425
           P S++EHSSI ATVK IF+LK FL+KRD WAGTFE V+ R SPR DCP TL+ P+KLR  
Sbjct: 364 PRSQYEHSSIPATVKTIFKLKDFLSKRDSWAGTFESVITRDSPRQDCPETLSTPIKLRGT 423

Query: 426 GANDTRRISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECE 485
            A +  ++SEFQE+LV +AA LKGD K E    KL +   V++A  Y  NA + FL E  
Sbjct: 424 MAKENAQLSEFQEDLVIMAAGLKGDYKNEELIHKLCKETCVADASKYVTNAFEKFLEESR 483

Query: 486 KARENGADESQIVVC 500
           KAR+ G DE+ IV C
Sbjct: 484 KARDRGCDENDIVYC 495

BLAST of Sgr025667 vs. TAIR 10
Match: AT3G03520.1 (non-specific phospholipase C3 )

HSP 1 Score: 674.9 bits (1740), Expect = 1.6e-193
Identity = 326/517 (63.06%), Postives = 389/517 (75.24%), Query Frame = 0

Query: 4   EITGTGGDGKATPNPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSE 63
           E T +GG   A  +PIKT+VVLVQENRSFDHMLGW K LN EIDGV+     SNP+STS+
Sbjct: 3   EETSSGGGSSA--SPIKTIVVLVQENRSFDHMLGWFKELNPEIDGVSESEPRSNPLSTSD 62

Query: 64  PNSPSVHFGNASGYVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNAERNSK 123
           PNS  + FG  S  +DPDPGHS Q IYEQ+FG+P+S+  +S    P M GF QNAE  +K
Sbjct: 63  PNSAQIFFGKESQNIDPDPGHSFQAIYEQVFGKPFSD--ESPYPDPKMNGFVQNAEAITK 122

Query: 124 GMSE-TVMNGFKPEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDT 183
           GMSE  VM GF PE + VFKELV EF VCDRWF+S+P+STQPNRLY+H+ATS+G  SNDT
Sbjct: 123 GMSEKVVMQGFPPEKLPVFKELVQEFAVCDRWFSSLPSSTQPNRLYVHAATSNGAFSNDT 182

Query: 184 KQLIGGLPQKTIFESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCR 243
             L+ G PQ+T+FESL+E GF FGIYYQ  P  L YRN+RKLKYV NFH + + FKRHC+
Sbjct: 183 NTLVRGFPQRTVFESLEESGFTFGIYYQSFPNCLFYRNMRKLKYVDNFHQYHLSFKRHCK 242

Query: 244 EGKLPNYVVIEQRYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITY 303
           EGKLPNYVVIE RYF + S P NDDHP +DV EGQ  +KE+YEALR+SPQWNEILF++ Y
Sbjct: 243 EGKLPNYVVIEPRYFKILSAPANDDHPKNDVVEGQNLVKEIYEALRASPQWNEILFVVVY 302

Query: 304 DEHGGFFDHVPTPVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVG 363
           DEHGG++DHVPTPV+GVPNPDGLVGP PYNFKFDRLGVRVP + +SPWIEPGTV+H P G
Sbjct: 303 DEHGGYYDHVPTPVIGVPNPDGLVGPEPYNFKFDRLGVRVPALLISPWIEPGTVLHEPNG 362

Query: 364 PQPTSEFEHSSIAATVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLR 423
           P+PTS+FEHSSI AT+KKIF LK FLTKRDEWAGT + V+NR SPRTDCPVTL +  + R
Sbjct: 363 PEPTSQFEHSSIPATLKKIFNLKSFLTKRDEWAGTLDAVINRTSPRTDCPVTLPELPRAR 422

Query: 424 DVG---ANDTRRISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSF 483
           D+      +   +++FQ EL+Q AAVLKGD  K++YP KL + M V +A  Y E A   F
Sbjct: 423 DIDIGTQEEDEDLTDFQIELIQAAAVLKGDHIKDIYPFKLADKMKVLDAARYVEEAFTRF 482

Query: 484 LHECEKARENGADESQIVVCGNQPQPSSKPKSFARSL 517
             E +KA+E G DE +IV         S PKSF + L
Sbjct: 483 HGESKKAKEEGRDEHEIVDLSKGSTRHSTPKSFVQKL 515

BLAST of Sgr025667 vs. TAIR 10
Match: AT3G03540.1 (non-specific phospholipase C5 )

HSP 1 Score: 670.2 bits (1728), Expect = 4.0e-192
Identity = 326/505 (64.55%), Postives = 382/505 (75.64%), Query Frame = 0

Query: 18  PIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPNSPSVHFGNASGY 77
           PIKT+VVLVQENRSFDH LGW K LN EIDGV   +Q  NP  +S+ NS +V FG+ S Y
Sbjct: 12  PIKTIVVLVQENRSFDHTLGWFKELNREIDGVMKSDQKFNPGFSSDLNSHNVVFGDQSQY 71

Query: 78  VDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPA-MRGFAQNAERNSKGMSETVMNGFKPE 137
           VDP+PGHSI+DIYEQ+FG+PW       N  PA M GFAQNAER  KGMS  VMNGFKP+
Sbjct: 72  VDPNPGHSIRDIYEQVFGKPWDSGHPDPNPGPATMSGFAQNAERKMKGMSSAVMNGFKPD 131

Query: 138 AVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQLIGGLPQKTIFE 197
           A+ V+KELV  F +CDRWFASVP +TQPNRL++HSATSHG ++N+ K LI G PQKTIFE
Sbjct: 132 ALPVYKELVQNFAICDRWFASVPGATQPNRLFIHSATSHGTTNNERKLLIEGFPQKTIFE 191

Query: 198 SLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREGKLPNYVVIEQRY 257
           SLDE GF FGIYYQ  P TL YRNLRKLKY+  FH + + FK+ C+EG LPNYVV+EQR+
Sbjct: 192 SLDEAGFTFGIYYQCFPTTLFYRNLRKLKYLTRFHDYGLQFKKDCKEGNLPNYVVVEQRW 251

Query: 258 FDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPTPV 317
           +DL   P NDDHPSHDVSEGQK +KEVYEALRSSPQWNEILF+ITYDEHGGF+DHVPTP+
Sbjct: 252 YDLLLNPANDDHPSHDVSEGQKLVKEVYEALRSSPQWNEILFIITYDEHGGFYDHVPTPL 311

Query: 318 VGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVGPQPTSEFEHSSIAA 377
            GVPNPDG++GPPPYNF+F+RLGVRVPT F+SPWIEPGTV+H   GP   S++EHSSI A
Sbjct: 312 DGVPNPDGILGPPPYNFEFNRLGVRVPTFFISPWIEPGTVLHGSNGPYLMSQYEHSSIPA 371

Query: 378 TVKKIFRLKQFLTKRDEWAGTFEIVLNRQSPRTDCPVTLNDPVKLRDVGANDTRRISEFQ 437
           TVKKIF+LK FLTKRD WAGTFE V+ R SPR DCP TL++PVK+R   A +   +S+FQ
Sbjct: 372 TVKKIFKLKDFLTKRDSWAGTFESVITRNSPRQDCPETLSNPVKMRGTVAKENAELSDFQ 431

Query: 438 EELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECEKARENGADESQI 497
           EELV +AA LKGD K E    KL +   VS+A  Y   A   F+ E +KARE G DE+ I
Sbjct: 432 EELVIVAAGLKGDYKNEELLYKLCKKTCVSDASKYVTKAFDKFVEESKKARERGGDENDI 491

Query: 498 VVCGN--------QPQPSSKPKSFA 514
           V C +        +P PS    S A
Sbjct: 492 VFCVDDDDDHNVVKPPPSQSEPSHA 516

BLAST of Sgr025667 vs. TAIR 10
Match: AT2G26870.1 (non-specific phospholipase C2 )

HSP 1 Score: 579.3 bits (1492), Expect = 9.3e-165
Identity = 291/486 (59.88%), Postives = 362/486 (74.49%), Query Frame = 0

Query: 17  NPIKTVVVLVQENRSFDHMLGWMKSLNSEIDGVTNDNQFSNPISTSEPNSPSVHFGNASG 76
           +PIKT+VV+V ENRSFDHMLGWMK LN EI+GV  D   SNP+S S+P+S  + FG+ S 
Sbjct: 25  SPIKTIVVVVMENRSFDHMLGWMKKLNPEINGV--DGSESNPVSVSDPSSRKIKFGSGSH 84

Query: 77  YVDPDPGHSIQDIYEQIFGEPWSEASQSKNLQPAMRGFAQNA--ERNSKGMSETVMNGFK 136
           YVDPDPGHS Q I EQ+FG     ++ +    P M GF Q A  E  S  MS +VMNGF+
Sbjct: 85  YVDPDPGHSFQAIREQVFG-----SNDTSMDPPPMNGFVQQAYSEDPSGNMSASVMNGFE 144

Query: 137 PEAVAVFKELVTEFGVCDRWFASVPASTQPNRLYLHSATSHGLSSNDTKQLIGGLPQKTI 196
           P+ V V+K LV+EF V DRWFASVP+STQPNR+++HS TS G +SN+   L  G PQ+TI
Sbjct: 145 PDKVPVYKSLVSEFAVFDRWFASVPSSTQPNRMFVHSGTSAGATSNNPISLAKGYPQRTI 204

Query: 197 FESLDEEGFNFGIYYQYLPATLLYRNLRKLKYVKNFHPFDIDFKRHCREGKLPNYVVIEQ 256
           F++LD+E F+FGIYYQ +PA L Y++LRKLKYV  FH +   FK H ++GKLP Y VIEQ
Sbjct: 205 FDNLDDEEFSFGIYYQNIPAVLFYQSLRKLKYVFKFHSYGNSFKDHAKQGKLPAYTVIEQ 264

Query: 257 RYFDLSSLPGNDDHPSHDVSEGQKFIKEVYEALRSSPQWNEILFLITYDEHGGFFDHVPT 316
           RY D    P +DDHPSHDV +GQKFIKEVYE LR+SPQWNE L +ITYDEHGG+FDHVPT
Sbjct: 265 RYMDTLLEPASDDHPSHDVYQGQKFIKEVYETLRASPQWNETLLIITYDEHGGYFDHVPT 324

Query: 317 PVVGVPNPDGLVGPPPYNFKFDRLGVRVPTVFVSPWIEPGTVVHRPVG-PQPTSEFEHSS 376
           PV  VP+PDG+VGP P+ F+F+RLG+RVPT+ VSPWIE GTVVH P G P P+SE+EHSS
Sbjct: 325 PVRNVPSPDGIVGPDPFLFQFNRLGIRVPTIAVSPWIEKGTVVHGPNGSPFPSSEYEHSS 384

Query: 377 IAATVKKIFRLKQ-FLTKRDEWAGTFEIVLN-RQSPRTDCPVTLNDPVKLRDVGANDTRR 436
           I ATVKK+F L   FLTKRDEWAGTFE +L  R+ PRTDCP TL +PVK+R   AN+   
Sbjct: 385 IPATVKKLFNLSSPFLTKRDEWAGTFENILQIRKEPRTDCPETLPEPVKIRMGEANEKAL 444

Query: 437 ISEFQEELVQLAAVLKGDDKKEVYPQKLVENMSVSEAVSYCENALKSFLHECEKARENGA 496
           ++EFQ+ELVQLAAVLKGD+    +P+++ + M+V E   Y E+A+K FL     A   GA
Sbjct: 445 LTEFQQELVQLAAVLKGDNMLTTFPKEISKGMTVIEGKRYMEDAMKRFLEAGRMALSMGA 503

Query: 497 DESQIV 498
           ++ ++V
Sbjct: 505 NKEELV 503