Sgr025623 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025623
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionserine/threonine-protein kinase BLUS1-like
Locationtig00152936: 1250396 .. 1253918 (+)
RNA-Seq ExpressionSgr025623
SyntenySgr025623
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAGCCCGAGAGTCCGAAATAGGAGGGCATCCATACGCAATTTCCAACGCCACAATCCCAATACCCCATACATCAGATTTGGAGCTGTACTCGCTGTCCGATCGTCCTACTACTTCAGGCGCAACCCAATACAGTAATGAAGAATACTCATCACTAGCCGCCTTTGGCCTATTGAAAACATTCACATCTGAGAGCTTGACCGTACCTTCCGAATCGACAAAAATGTTACTTGGTTTGATGTCTTTATGAACGTAGCCAAGTTGATGAAGACAAGCCAAGCCAGAGAGAAGTTGATGAAGGAGTATGGCCAAGAATTGATCAGGTAAGCCGTTTTGGAAACGAGCCGCCATTAGGGTCTCCAAAGATCCAACATGGATGAAAGGCATCACGATCCAAAGTTGACGATCCATCATGAAAGAACAGTGAGGGCTAAGAATATTAGGATGTTGGATAAGGGAGAGGGAGTTTATAATATTCTTCACATCATCCCGATCCCAATCCAATCCATCCATATCCACAACTTTCACAGCCACCGTGGTCGAGTTTACCGACTCGCAAATCGCCTTGTAAACAATACTATGCTCACTAATACCGATTTGACATAGAATCTTGTAGCAGTTGGCGTCCAATGGAAATTTGGCCATTGTACACAAACTAAGGGATTGGTAATAAGACGATGGAGATGTTGTGTCTTTTCATTATGTTGCAATTAATTATATAACATTTCTATTTTACTGTTTCAATTTGGTCTGGAAACCACTCACTCTTCCCATGCCTATTATGTAGGAAGTGGTTGACTAATACAAAGGAGAGAGAGAGAATTTGTAGGGTTCAAAATCAAATGATCCTTTTTCCTCGAAAAAAGAATTACAAGGTTTCTATGTTACGTTATTTTTTTCATCTTGAACTATTCTTGTGTCTTGAGGTTTCTGTGCAATTCAACAGGACCCACAATTTCACAAAAGCATATTACTCATACCTATTTTAAAAAAAAAAAAAAAAGAAGAAGAAATATTGTCAACGAAAAGAATTTTGTAAATTCAATTTTTGATGATGGGAAATACAAATCTTATTAGTTCTTGGTTGCTCAAGACTATTTTACACAATTTATTTTAGGTTCGTAAAAATATTTATTTTATATATTCGTGAAAATTTTATTACTCTTATTATTAAGGTTTATTAAGATTATTTTTTTCATTTGTAAAAATAAGCCATTATTATAAATGAAATGTTAAATTTATACAAAATGCTCCTAAACTTTGAGGTAGATTTTAATTACGCTCCTATCGTTTCAAAAATATCCTTAAGTTTTAAATTTTTTTAAAAAAATACCATTCAATTAATTTTTCTATTAAAATGAAGACAAAAATATGACGTGATGGCGAGTTGTAAAAATGGAGGTTACACGTGACAGTGAGCTGTAAAAAAAAAGTTTCAATTATGCTCCTGTAGTTTAAAAGTTTTATTTGAACTCCTAACCTTTAAAGTTTCATTTTCGTCTTCATTTTAGTGTAAAAGTTAATGGAATGATAATTTTAAACAATTTTAAAGGTTAAGAATATTTTTGAAACTTTTAGAACGATAAGAGCATAATTAAAACCTACCTCAAAGTTCAAGGACGTTTTGTATAATTTAACCTAAATGAAAAATAATAAATATTTGAAAGGGAGAAACTTTATAAAATTTGCTTTTAAAAGAAAAAAGTTATTTGAAAATTGATAATATTATTGATTTAATTATCTTTTCTCCTGTTAATGATTTCAAAAAGGGAAAAAAGTGCTTAAATTTTGAAAATTTATGATTAAATTATTTTTCTTCTTCTTATTATTGTTTTTGGTTGATTCCAAAAAGAAGAGAGTGTTTTTTATTGAATATCTTTGTGGAGGAAAAAGGATAATTTAGACTCTAAAAATGCTTTTTTTTTTTAATGAGTCAACCACTTCCAACACAATAGGCATATGAAGAGTAGCTAATATTCCAACAGATTGAGCGACCGCTTAGCGTAGGTTAAAAGAGAGAAATGTTATAAATTTGCAACAAAATGAAAAGACAATAATATCAATGCTTGTGTAATGGTAATGGTCTAATGGAGCAGGTTCAAGACCAAGGCAAGGCCGTCGGCGCCGGTCGGGCTGAATTCCCTATGGATGCTGACGGCTACAAGATTCTCTGTCAGATCGGTGTTAGTGAGCACAGTGTGGTTTACAAGGCGATTTGCGAGTCGGTGAACTCGACCACGGTGGCCGTGAAAGTTGTGGATATGGATGGATTGGATTGGGATCATGTGAAGAATATTATCAACTCCCTCTCCCTTATCCAACATCCTAATATTCTTAGCCCTCACTGTTCTTTCATGATGGATCGTCAACTTTGGATCGTGATGCCTTTCATCCATGTTGGATCTTTGGAGACCCTAATGGCGGCTCGTTTCCAAAACGGCTTACCAGATCAATTCTTGGCCATACTCCTTCATCAACTTCTCTCTGGCTTGGCGTGTCTTCACCAACTTGGCTACGTTCATAACGACATCAAACCAAGTAATATTTTCATTGATTCCGAAGGTACGGTTAAGCTCTCAGATGTGAATGTTTTCAAGCCAACGACGTGGGCTAGTGATGAATATTCTTCTTCATTACCATATTGGGTTGCGCCGGAACTAGGAGGATCGGATGGCGAGTACAGTTCCAAATCTGATATATGGGCTATTGGGATTGTGGCGTTGGAAATAACATCGGGATGCCTTCTTGTTTCAAATTTCTTGATTCTTAGATCCCAGTTCTTGAAGATTTCGCGGCGGCTCCCATACCGTTTGAATATGTATAACTTTTTGAGCAAGGACGCCATAGACTGGAAGTTCTCATTATCGGATTCATTCAAAGATTTCGTGAGCTATTGTCTTGTTAAAGATCCGTCGGAGAGGGGTTCGGCAGAGAGTTTATTGGGGCATCCATTCATCATGGAGCAAGAGGAGAGTAGAAACAATTGTTTTGTATCATTGAAGAGTATTGTAAAAGATATGGTTGGAATCGAGGATATGTTTGAAACGAATAAGGATCGACTGGAGTTTTTAATGGCGAATGGGAGAAAAGAGATAGACAACAAAGAAAATGGGCAGATGGTAAAGATAGATCGGTGGGATTTCAACAAGTCCCACTTCCAACTAGAGCCAATAAAGGAAGAAACGTTAGCGGAGAAGGTGGGAACCATCATTATTGCAGAGGAAGGCAAAGAAGAAGGTGGTAGACGAACAGTTGATGAAAAATTAGTGTTGTCTTCATTGAGAGAAAGCTTAAACAAGGTGATAGCTCAGTTTGGTTTGTTTTTTAAAAGAGAGTTGGAAATGACGAGGGAAATAGAGAGGTTGAAGGCCGAGTTGGGGGCTGAGAAGGAGAGAAGCTTTGAGTTGGAGTTAGTAATGAGGAGGGAAATACAGAGGGTGAAGGCCGAGTTGGGGGCCGAGAAGCACAGAAACTTTCACTTTGCCTTGGCTTCCTTGTCACCAACTCCAAAACAATTCTAA

mRNA sequence

ATGAGAGCCCGAGAGTCCGAAATAGGAGGGCATCCATACGCAATTTCCAACGCCACAATCCCAATACCCCATACATCAGATTTGGAGCTGTACTCGCTGTCCGATCGTCCTACTACTTCAGGCGCAACCCAATACACCAAGTTGATGAAGACAAGCCAAGCCAGAGAGAAGTTGATGAAGGAGTATGGCCAAGAATTGATCAGAACAGTGAGGGCTAAGAATATTAGGATGTTGGATAAGGGAGAGGGAGTTTATAATATTCTTCACATCATCCCGATCCCAATCCAATCCATCCATATCCACAACTTTCACAGCCACCGTGGTCGAGTTCAAGACCAAGGCAAGGCCGTCGGCGCCGGTCGGGCTGAATTCCCTATGGATGCTGACGGCTACAAGATTCTCTGTCAGATCGGTGTTAGTGAGCACAGTGTGGTTTACAAGGCGATTTGCGAGTCGGTGAACTCGACCACGGTGGCCGTGAAAGTTGTGGATATGGATGGATTGGATTGGGATCATGTGAAGAATATTATCAACTCCCTCTCCCTTATCCAACATCCTAATATTCTTAGCCCTCACTGTTCTTTCATGATGGATCGTCAACTTTGGATCGTGATGCCTTTCATCCATGTTGGATCTTTGGAGACCCTAATGGCGGCTCGTTTCCAAAACGGCTTACCAGATCAATTCTTGGCCATACTCCTTCATCAACTTCTCTCTGGCTTGGCGTGTCTTCACCAACTTGGCTACGTTCATAACGACATCAAACCAAGTAATATTTTCATTGATTCCGAAGGTACGGTTAAGCTCTCAGATGTGAATGTTTTCAAGCCAACGACGTGGGCTAGTGATGAATATTCTTCTTCATTACCATATTGGGTTGCGCCGGAACTAGGAGGATCGGATGGCGAGTACAGTTCCAAATCTGATATATGGGCTATTGGGATTGTGGCGTTGGAAATAACATCGGGATGCCTTCTTGTTTCAAATTTCTTGATTCTTAGATCCCAGTTCTTGAAGATTTCGCGGCGGCTCCCATACCGTTTGAATATGTATAACTTTTTGAGCAAGGACGCCATAGACTGGAAGTTCTCATTATCGGATTCATTCAAAGATTTCGTGAGCTATTGTCTTGTTAAAGATCCGTCGGAGAGGGGTTCGGCAGAGAGTTTATTGGGGCATCCATTCATCATGGAGCAAGAGGAGAGTAGAAACAATTGTTTTGTATCATTGAAGAGTATTGTAAAAGATATGGTTGGAATCGAGGATATGTTTGAAACGAATAAGGATCGACTGGAGTTTTTAATGGCGAATGGGAGAAAAGAGATAGACAACAAAGAAAATGGGCAGATGGTAAAGATAGATCGGTGGGATTTCAACAAGTCCCACTTCCAACTAGAGCCAATAAAGGAAGAAACGTTAGCGGAGAAGGTGGGAACCATCATTATTGCAGAGGAAGGCAAAGAAGAAGGTGGTAGACGAACAGTTGATGAAAAATTAGTGTTGTCTTCATTGAGAGAAAGCTTAAACAAGGTGATAGCTCAGTTTGGTTTGTTTTTTAAAAGAGAGTTGGAAATGACGAGGGAAATAGAGAGGTTGAAGGCCGAGTTGGGGGCTGAGAAGGAGAGAAGCTTTGAGTTGGAGTTAGTAATGAGGAGGGAAATACAGAGGGTGAAGGCCGAGTTGGGGGCCGAGAAGCACAGAAACTTTCACTTTGCCTTGGCTTCCTTGTCACCAACTCCAAAACAATTCTAA

Coding sequence (CDS)

ATGAGAGCCCGAGAGTCCGAAATAGGAGGGCATCCATACGCAATTTCCAACGCCACAATCCCAATACCCCATACATCAGATTTGGAGCTGTACTCGCTGTCCGATCGTCCTACTACTTCAGGCGCAACCCAATACACCAAGTTGATGAAGACAAGCCAAGCCAGAGAGAAGTTGATGAAGGAGTATGGCCAAGAATTGATCAGAACAGTGAGGGCTAAGAATATTAGGATGTTGGATAAGGGAGAGGGAGTTTATAATATTCTTCACATCATCCCGATCCCAATCCAATCCATCCATATCCACAACTTTCACAGCCACCGTGGTCGAGTTCAAGACCAAGGCAAGGCCGTCGGCGCCGGTCGGGCTGAATTCCCTATGGATGCTGACGGCTACAAGATTCTCTGTCAGATCGGTGTTAGTGAGCACAGTGTGGTTTACAAGGCGATTTGCGAGTCGGTGAACTCGACCACGGTGGCCGTGAAAGTTGTGGATATGGATGGATTGGATTGGGATCATGTGAAGAATATTATCAACTCCCTCTCCCTTATCCAACATCCTAATATTCTTAGCCCTCACTGTTCTTTCATGATGGATCGTCAACTTTGGATCGTGATGCCTTTCATCCATGTTGGATCTTTGGAGACCCTAATGGCGGCTCGTTTCCAAAACGGCTTACCAGATCAATTCTTGGCCATACTCCTTCATCAACTTCTCTCTGGCTTGGCGTGTCTTCACCAACTTGGCTACGTTCATAACGACATCAAACCAAGTAATATTTTCATTGATTCCGAAGGTACGGTTAAGCTCTCAGATGTGAATGTTTTCAAGCCAACGACGTGGGCTAGTGATGAATATTCTTCTTCATTACCATATTGGGTTGCGCCGGAACTAGGAGGATCGGATGGCGAGTACAGTTCCAAATCTGATATATGGGCTATTGGGATTGTGGCGTTGGAAATAACATCGGGATGCCTTCTTGTTTCAAATTTCTTGATTCTTAGATCCCAGTTCTTGAAGATTTCGCGGCGGCTCCCATACCGTTTGAATATGTATAACTTTTTGAGCAAGGACGCCATAGACTGGAAGTTCTCATTATCGGATTCATTCAAAGATTTCGTGAGCTATTGTCTTGTTAAAGATCCGTCGGAGAGGGGTTCGGCAGAGAGTTTATTGGGGCATCCATTCATCATGGAGCAAGAGGAGAGTAGAAACAATTGTTTTGTATCATTGAAGAGTATTGTAAAAGATATGGTTGGAATCGAGGATATGTTTGAAACGAATAAGGATCGACTGGAGTTTTTAATGGCGAATGGGAGAAAAGAGATAGACAACAAAGAAAATGGGCAGATGGTAAAGATAGATCGGTGGGATTTCAACAAGTCCCACTTCCAACTAGAGCCAATAAAGGAAGAAACGTTAGCGGAGAAGGTGGGAACCATCATTATTGCAGAGGAAGGCAAAGAAGAAGGTGGTAGACGAACAGTTGATGAAAAATTAGTGTTGTCTTCATTGAGAGAAAGCTTAAACAAGGTGATAGCTCAGTTTGGTTTGTTTTTTAAAAGAGAGTTGGAAATGACGAGGGAAATAGAGAGGTTGAAGGCCGAGTTGGGGGCTGAGAAGGAGAGAAGCTTTGAGTTGGAGTTAGTAATGAGGAGGGAAATACAGAGGGTGAAGGCCGAGTTGGGGGCCGAGAAGCACAGAAACTTTCACTTTGCCTTGGCTTCCTTGTCACCAACTCCAAAACAATTCTAA

Protein sequence

MRARESEIGGHPYAISNATIPIPHTSDLELYSLSDRPTTSGATQYTKLMKTSQAREKLMKEYGQELIRTVRAKNIRMLDKGEGVYNILHIIPIPIQSIHIHNFHSHRGRVQDQGKAVGAGRAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMDGLDWDHVKNIINSLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVKIDRWDFNKSHFQLEPIKEETLAEKVGTIIIAEEGKEEGGRRTVDEKLVLSSLRESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERSFELELVMRREIQRVKAELGAEKHRNFHFALASLSPTPKQF
Homology
BLAST of Sgr025623 vs. NCBI nr
Match: XP_022138626.1 (serine/threonine-protein kinase BLUS1-like [Momordica charantia])

HSP 1 Score: 414.5 bits (1064), Expect = 1.6e-111
Identity = 260/491 (52.95%), Postives = 309/491 (62.93%), Query Frame = 0

Query: 111 QDQGKAVGAGRA--EFPMDADGYKILCQIGVSEHSVVYKAICESV-NSTTVAVKVVDMDG 170
           QD   A  AGR   +FP  ADGYKIL QIGVS+HSVVYKAICES  NS  VAVKV+D+D 
Sbjct: 8   QDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDVDR 67

Query: 171 LDWDHVKNIINSLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPD 230
              +     +NSLSLI H NILSPHC F +DR+LW VMPFIH G L+ ++A RF NGLPD
Sbjct: 68  HVDEFFVMKMNSLSLIDHANILSPHCCFTVDRRLWTVMPFIHAGPLQAILANRFPNGLPD 127

Query: 231 QFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSS 290
           QFLAILLHQLL  L CLH+LGYVH +IK SNIF+DSEG+VKLSD+NV+       D+  S
Sbjct: 128 QFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVY---VGDDDDDDS 187

Query: 291 SLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYR 350
           S+PYW  P     D EYS KSD+WAIGIVALE+  GC  +S+   +RSQ L ISRR PY 
Sbjct: 188 SVPYWDPP-----DEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYN 247

Query: 351 LNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCF 410
           LN+    SK  ID KF   + FKDFVS CLVKDPS+R SA SLL HPFI + E S     
Sbjct: 248 LNLDKMSSKSVIDRKF--GELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSN---- 307

Query: 411 VSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVKIDRWDFNKSHFQLEP 470
           V  KSI KDM GIED FE NKDRL  LM   R+  DN   G +VK+  WDFN   F+LEP
Sbjct: 308 VLFKSIAKDMAGIEDRFEKNKDRLVELMKKDRRVEDN-NRGNVVKVSGWDFNNFLFELEP 367

Query: 471 IKEETLAEKV----GTII--------IAEEGK---------------------------- 530
             EE+L ++V     TII        + E G+                            
Sbjct: 368 -DEESLGKRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSGRMGSR 427

Query: 531 -EEGGRRTVDEKL--VLSSLRESLNK-------VIAQFGLFFKRELEMTREIERLKAELG 549
            EE  R    E L  VL+SL ES+++       VI+QFG   +R++EMT+EI++LKAEL 
Sbjct: 428 AEEEVRVMDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKAELA 482

BLAST of Sgr025623 vs. NCBI nr
Match: XP_023006000.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita maxima])

HSP 1 Score: 322.4 bits (825), Expect = 8.1e-84
Identity = 203/480 (42.29%), Postives = 293/480 (61.04%), Query Frame = 0

Query: 107 RGRVQDQGKAVGAG-RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNST-TVAVKVVD 166
           R ++QDQ  +   G   +FP  AD Y+I  QIG S+HS VYKAIC+S +ST  VA+K++D
Sbjct: 3   RRQIQDQETSAAKGTHFKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIKIID 62

Query: 167 MD-GLDWDHVKNIINSLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQ 226
            +  +D   + NI  +LS I HP+ILSPHC F+  D  LWIVMPFIH G L+ +++ RF+
Sbjct: 63  SNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQRFR 122

Query: 227 NGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWAS 286
            GLPDQF++IL++QLL  L  LH+ G VH  +K SNIF D+ G V LSD++V K      
Sbjct: 123 TGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVSKD----M 182

Query: 287 DEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISR 346
           ++  SS+PYWV PE      +YSSKSD+W++GI+A+EI  GC  + +  ++++QF KISR
Sbjct: 183 NKDCSSVPYWVPPE-----EDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 242

Query: 347 RLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEES 406
           R PY LN+     K  +  KF   D+ K FV  CL KDP  R SA SLL HPF+ + E S
Sbjct: 243 RFPYDLNLDTRSGKSVVPRKF--GDTLKHFVGSCLAKDPFARVSALSLLDHPFVKKGENS 302

Query: 407 RNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKE---NGQMVKIDRWDFN 466
                   KSI K MVGI +MFE +KDR+  LM N  +  ++ +    G++++I  WD+N
Sbjct: 303 N----FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGGGKVLRITGWDYN 362

Query: 467 KSHFQLEPIKEETLAEKV---GTIIIAEEGKEE-----------GG---RRTVDEKL--- 526
              F+LEP  EE+  ++V   G  + + + +E+           GG   ++ VD+ +   
Sbjct: 363 NFLFELEP-DEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSDEKKEVDQAMDSE 422

Query: 527 ----VLSSL-------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERSFELEL 549
               +LSSL       +E ++ ++   G    ++LEM ++I+RL+ EL AEKE+SF+LEL
Sbjct: 423 TLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Sgr025623 vs. NCBI nr
Match: XP_023548299.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 313.2 bits (801), Expect = 4.9e-81
Identity = 199/485 (41.03%), Postives = 291/485 (60.00%), Query Frame = 0

Query: 107 RGRVQDQ-------GKAVGAGRAEFPMDADGYKILCQIGVSEHSVVYKAICESVNS-TTV 166
           R ++QDQ         A     ++FP  AD Y+I  QIG S+HS VYKAIC+S ++ T V
Sbjct: 3   RRQIQDQETSAASSASAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIV 62

Query: 167 AVKVVDMD-GLDWDHVKNIINSLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETL 226
           A+K++D +  +D   + NI  +LS I HP+IL+PHC F   D +LWIVMPFIH G L+ +
Sbjct: 63  AIKIIDSNVEIDPTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIHAGPLQAI 122

Query: 227 MAARFQNGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFK 286
           ++ RF+ GLPDQF++IL++QLL  L  LH+ G VH  +K SNIF D+ G V LSD++V K
Sbjct: 123 ISQRFRTGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSK 182

Query: 287 PTTWASDEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQ 346
                 ++  SS+PYW  PE      +YS KSD+W++GI+A+EI  GC  + +  ++++Q
Sbjct: 183 ----EMNKDCSSVPYWDPPE-----EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQ 242

Query: 347 FLKISRRLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFI 406
           F KISRR PY LN+ N   K  +  KF   D+ K FV  CL KD   R SA SLL HPF+
Sbjct: 243 FSKISRRFPYDLNLDNRSGKSVVPRKF--GDTLKHFVGSCLAKDRFARASALSLLDHPFV 302

Query: 407 MEQEESRNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKE-NGQMVKIDR 466
            + E S        KSI K MVGI +MFE +KDR+  LM N  +  ++ +  G++++I  
Sbjct: 303 KKGENSN----FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITG 362

Query: 467 WDFNKSHFQLEPIKEETLAEKV---GTIIIAEEGKEE-----------GG----RRTVDE 526
           WD+N   F+LEP  EE+  ++V   G  + + + +E+           GG    ++ VD+
Sbjct: 363 WDYNNFLFELEP-DEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKEVDQ 422

Query: 527 KL-------VLSSL-------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERS 549
            +       +LSSL       +E ++ ++   G    ++LEM ++I+RL+ EL AEKE+S
Sbjct: 423 AMDSETLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKS 471

BLAST of Sgr025623 vs. NCBI nr
Match: XP_022958864.1 (serine/threonine-protein kinase BLUS1-like [Cucurbita moschata])

HSP 1 Score: 312.8 bits (800), Expect = 6.4e-81
Identity = 195/479 (40.71%), Postives = 282/479 (58.87%), Query Frame = 0

Query: 107 RGRVQDQGKAVGAG-RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNS-TTVAVKVVD 166
           R ++QDQ  +   G  ++FP  AD Y+I  QIG S+HS VYKAIC+S ++ T VA+K++D
Sbjct: 3   RRQIQDQETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIID 62

Query: 167 MD-GLDWDHVKNIINSLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQ 226
            +  +D   + NI  +LS I HP+IL+PHC F   D +LWIVMPFI  G L+ +++ RF+
Sbjct: 63  SNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFR 122

Query: 227 NGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWAS 286
            GLPDQF++IL++QLL  L  LH+ G VH  +K SNIF D+ G V LSD++V K      
Sbjct: 123 TGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSK----EM 182

Query: 287 DEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISR 346
            +  SS+PYW  PE      +YS KSD+W++GI+A+EI  GC  + +  ++++QF KISR
Sbjct: 183 KKDCSSVPYWDPPE-----EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 242

Query: 347 RLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEES 406
           R PY LN+ N   K  +  KF   D+ K FV  CL KD   R SA SLL HPF+ + E S
Sbjct: 243 RFPYDLNLDNRSGKSVVPRKF--GDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENS 302

Query: 407 RNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKE-NGQMVKIDRWDFNKS 466
                   KSI K MVGI +MFE +KDR+  LM N  +  ++ +  G++++I  WD+N  
Sbjct: 303 N----FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNF 362

Query: 467 HFQLEP------------------IKEETLAEKVGTIIIAEEGKEEGGRRTVDEKL---- 526
            F+LEP                   KE+    K     +   G  +  ++ VD+ +    
Sbjct: 363 LFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQAMDTET 422

Query: 527 ---VLSSL-------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERSFELEL 549
              +LSSL       +E ++ ++   G    ++LEM ++I+RL+ EL AEKE+SF+LEL
Sbjct: 423 LATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Sgr025623 vs. NCBI nr
Match: KAG6574930.1 (Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.8 bits (800), Expect = 6.4e-81
Identity = 195/479 (40.71%), Postives = 282/479 (58.87%), Query Frame = 0

Query: 107 RGRVQDQGKAVGAG-RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNS-TTVAVKVVD 166
           R ++QDQ  +   G  ++FP  AD Y+I  QIG S+HS VYKAIC+S ++ T VA+K++D
Sbjct: 3   RRQIQDQETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIID 62

Query: 167 MD-GLDWDHVKNIINSLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQ 226
            +  +D   + NI  +LS I HP+IL+PHC F   D +LWIVMPFI  G L+ +++ RF+
Sbjct: 63  SNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFR 122

Query: 227 NGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWAS 286
            GLPDQF++IL++QLL  L  LH+ G VH  +K SNIF D+ G V LSD++V K      
Sbjct: 123 TGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSK----EM 182

Query: 287 DEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISR 346
            +  SS+PYW  PE      +YS KSD+W++GI+A+EI  GC  + +  ++++QF KISR
Sbjct: 183 KKDCSSVPYWDPPE-----EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 242

Query: 347 RLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEES 406
           R PY LN+ N   K  +  KF   D+ K FV  CL KD   R SA SLL HPF+ + E S
Sbjct: 243 RFPYDLNLDNRSGKSVVPRKF--GDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENS 302

Query: 407 RNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKE-NGQMVKIDRWDFNKS 466
                   KSI K MVGI +MFE +KDR+  LM N  +  ++ +  G++++I  WD+N  
Sbjct: 303 N----FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNF 362

Query: 467 HFQLEP------------------IKEETLAEKVGTIIIAEEGKEEGGRRTVDEKL---- 526
            F+LEP                   KE+    K     +   G  +  ++ VD+ +    
Sbjct: 363 LFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQAMDTET 422

Query: 527 ---VLSSL-------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERSFELEL 549
              +LSSL       +E ++ ++   G    ++LEM ++I+RL+ EL AEKE+SF+LEL
Sbjct: 423 LATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Sgr025623 vs. ExPASy Swiss-Prot
Match: O23304 (Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 PE=1 SV=1)

HSP 1 Score: 238.4 bits (607), Expect = 2.0e-61
Identity = 163/487 (33.47%), Postives = 269/487 (55.24%), Query Frame = 0

Query: 121 RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMD--GLDWDHVKNIIN 180
           + EFP+DA+ Y+I+C+IGV   + VYKAIC  +NS  VA+K +D+D    D+D ++    
Sbjct: 5   KLEFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETK 64

Query: 181 SLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLL 240
           ++SL+ HPNIL+ +CSF +DR LW+VMPF+  GSL +++++ F +GLP+  +++ L + L
Sbjct: 65  TMSLLSHPNILNAYCSFTVDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETL 124

Query: 241 SGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSD----VNVFKPTTWASDEYSSSL----- 300
           + ++ LH  G++H DIK  NI +DS+G+VKL+D     ++++P T +S   SSSL     
Sbjct: 125 NAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTDI 184

Query: 301 ---PYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRL-- 360
              PYW+APE+  S   Y  K+DIW+ GI ALE+  G   +S+   L+S  +KI++R   
Sbjct: 185 AGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRFHF 244

Query: 361 -PYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESR 420
             Y +N      K   + KF  S +F++ V  CL +DP++R SAE LL HPF    +  +
Sbjct: 245 SDYEINTSGSSKKG--NKKF--SKAFREMVGLCLEQDPTKRPSAEKLLKHPFF---KNCK 304

Query: 421 NNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVK---IDRWDFNK 480
              FV +K+++  +   E MF  ++  ++ +  +  +E   +E+ ++VK   I  W+F +
Sbjct: 305 GLDFV-VKNVLHSLSNAEQMFMESQILIKSVGDDDEEE--EEEDEEIVKNRRISGWNFRE 364

Query: 481 SHFQLEPI-------------KEETLAE-------------KVGTIIIAEEGK-EEGGRR 540
              QL P+             +EE  ++             ++G  +  EE K +EG   
Sbjct: 365 DDLQLSPVFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAKNQEGEVV 424

Query: 541 TVDEKLVLSS---LRESLNKVIAQFGLFFK----------RELEMTREIERLKAELGAEK 548
             D+ LVL     L++SL    A+  +  +          RE E+   +E+LK EL  EK
Sbjct: 425 GFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELLEMVEKLKIELETEK 481

BLAST of Sgr025623 vs. ExPASy Swiss-Prot
Match: Q551H4 (Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fray2 PE=3 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 2.3e-33
Identity = 90/287 (31.36%), Postives = 137/287 (47.74%), Query Frame = 0

Query: 121 RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMD---GLDWDHVKNII 180
           R  +P  AD Y++   IG     +V +AIC       VA+K++D++    +  + ++  I
Sbjct: 61  RKNYPNSADQYELKETIGKGGSGLVQRAICLPFQE-NVAIKIIDLEHCKNVSLEEIRKEI 120

Query: 181 NSLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQL 240
            ++SL  HPN+++ H SF+ +  LW++M F+  GS   +M   F  G  +  +A +L + 
Sbjct: 121 QAMSLCHHPNVVAYHTSFVYNESLWVIMDFLSAGSCSDIMRFSFPQGFEEHVIATILKEA 180

Query: 241 LSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNV---FKPTTWASDEYSSSLPYWVA 300
           L  +   H+ G +H DIK  NI IDS G ++LSD  V      T   S       P W+A
Sbjct: 181 LKAICYFHKTGRIHRDIKSGNILIDSNGNIQLSDFGVSATLIDTGETSRNTFVGTPCWMA 240

Query: 301 PELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFL 360
           PE+      Y    DIW+ GI ALE+  G          ++ F +        L + N  
Sbjct: 241 PEI-MEQVNYDYAVDIWSFGITALELARG----------KAPFAEYPPMKVLLLTLQNPP 300

Query: 361 SKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEE 402
                D +   S SFKD V  CL KDPS+R     LL H F  + ++
Sbjct: 301 PSLEGDGESKWSHSFKDLVEKCLQKDPSKRPLPSKLLEHRFFKQAKK 335

BLAST of Sgr025623 vs. ExPASy Swiss-Prot
Match: Q54XL6 (Serine/threonine-protein kinase fray1 OS=Dictyostelium discoideum OX=44689 GN=fray1 PE=3 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 3.9e-33
Identity = 93/294 (31.63%), Postives = 149/294 (50.68%), Query Frame = 0

Query: 122 AEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMDGLDWDHVKNIINSLS 181
           +++P  A+ Y ++  IG      V+KA C  +    VA+KVV++D +D   VK++I  + 
Sbjct: 88  SDYPSQANQYNLIEPIGEGTEGRVFKAYCIPLKE-NVAIKVVELDKMDPQFVKDVIKEVK 147

Query: 182 LI---QHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLL 241
           ++    HPN++  H SF+ + QLW+VM ++  GSL  +M  ++ +G+P+     +L  LL
Sbjct: 148 VMNGNNHPNLIHYHTSFLENNQLWLVMDYLGGGSLADIMKFKYPDGIPEVLAVTVLKSLL 207

Query: 242 SGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNV---FKPTTWASDEYSSSLPYWVAP 301
            GL  LH    +H D+K  NI I  +G ++L+D  V   F+  T  S +     P W+AP
Sbjct: 208 KGLEYLHSHQRIHRDLKSDNILIGEDGAIELADFGVSAMFEKNTCCSRKTIVGTPCWMAP 267

Query: 302 ELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLS 361
           E+    G Y+   DIW+ GI A+E+  G             +     ++   L   N  S
Sbjct: 268 EIISERG-YNQGVDIWSFGITAIELIRG---------KPPGYDLPPSKVFMNLLFGNSPS 327

Query: 362 KDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVS 410
               + K   S  +KD V  CL K+PS+R +A  LL H     ++  +NN  VS
Sbjct: 328 LQEEEDKGVCSHLYKDLVDKCLQKEPSKRPNASKLLEHKVF--KQAKKNNYIVS 368

BLAST of Sgr025623 vs. ExPASy Swiss-Prot
Match: Q86IX1 (Serine/threonine-protein kinase dst1 OS=Dictyostelium discoideum OX=44689 GN=dst1 PE=3 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 9.7e-32
Identity = 94/300 (31.33%), Postives = 152/300 (50.67%), Query Frame = 0

Query: 131 YKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMDGLDWDHVKNIINSLSLI---QHPN 190
           Y I  ++G      V+KA+    N   VA+K++ +D  D + +K++   +S++    +PN
Sbjct: 29  YHIQERLGKGSFGQVFKAV-HFANGKVVAIKIISLD--DQEAIKDVRKEISILAECNYPN 88

Query: 191 ILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLLSGLACLHQL 250
           I+    S+  D QLWIVM +   GS+  L+  +  + + +  +A++  + L GL  LH+ 
Sbjct: 89  IVQYFGSYFKDHQLWIVMEYCGGGSVSDLL--QVIDTISEDEIALICREALKGLNYLHEF 148

Query: 251 GYVHNDIKPSNIFIDSEGTVKLSDVNVFKP--TTWASDEYSSSLPYWVAPELGGSDGEYS 310
             +H DIK  NI ++  G VKL+D  V      T++        PYW+APE+   + +Y 
Sbjct: 149 KKIHRDIKGGNILLNDRGEVKLADFGVSAQLFNTFSKRNTFVGTPYWMAPEV-IQENKYD 208

Query: 311 SKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLSKDAIDWKFSL 370
            K+D+W++GI A+E+  G    SN   +R  F+      P            A+  K   
Sbjct: 209 GKADVWSLGITAIEMAEGLPPNSNVHPMRVIFMIPREESP------------ALTDKSIW 268

Query: 371 SDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLKSIVKDMVGIEDMFE 426
           SD F+DF+S CL KDP+ER +A+ LL H FI  Q +   +    L    K ++    MFE
Sbjct: 269 SDKFQDFISKCLTKDPAERPTAKELLNHEFI--QTKKPLSILSDLVENCKTLINNSSMFE 308

BLAST of Sgr025623 vs. ExPASy Swiss-Prot
Match: P08458 (Sporulation-specific protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SPS1 PE=1 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 2.0e-29
Identity = 105/349 (30.09%), Postives = 174/349 (49.86%), Query Frame = 0

Query: 131 YKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMDGLDWD--HVKNIINSLSLIQHPNI 190
           Y I   IG      VYKA+ + V    VA+KVV+++  D D   +   I  L+ ++ P I
Sbjct: 18  YSIQSCIGRGNFGDVYKAV-DRVTQEIVAIKVVNLEHSDEDIELLAQEIFFLAELKSPLI 77

Query: 191 LSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLLSGLACLHQLG 250
            +   + + D  +WIVM +   GS   L+   + NGLP++ ++ ++H++  GL  LH+  
Sbjct: 78  TNYIATMLEDVSMWIVMEYCGGGSCSDLLKRSYVNGLPEEKVSFIIHEVTLGLKYLHEQR 137

Query: 251 YVHNDIKPSNIFIDSEGTVKLSDVNV--FKPTTWASDEYSSSLPYWVAPELGGSDGE-YS 310
            +H DIK +NI ++ EG VKL D  V     +T   D +  + PYW+APE+   + + Y+
Sbjct: 138 KIHRDIKAANILLNEEGMVKLGDFGVSGHIRSTLKRDTFVGT-PYWMAPEVVCCEVDGYN 197

Query: 311 SKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLSKDAIDWKFSL 370
            K+DIW++GI   E+  G   +S +       +K+   LP R           +   F  
Sbjct: 198 EKADIWSLGITTYELLKGLPPLSKY-----DPMKVMTNLPKR-------KPPKLQGPF-- 257

Query: 371 SDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLKSIVKDMVGIEDMFE 430
           SD+ KDFV+ CLVK P++R SA +LL   F+      +N    +LKS V D++  + + E
Sbjct: 258 SDAAKDFVAGCLVKTPADRPSAYNLLSFEFV------KNITITNLKSDV-DLIKQKKVQE 317

Query: 431 TNKDRLEFLMANGRKEIDNKENGQMVKIDRWDFNKSHFQLEPIKEETLA 475
                 ++ + N   +  N   G+    + W+F  +      I +E L+
Sbjct: 318 RYTKVPKYPLQNRLYKNSNTVRGK----EFWNFESTRLSTTQISKEELS 339

BLAST of Sgr025623 vs. ExPASy TrEMBL
Match: A0A6J1CA92 (serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC111009741 PE=4 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 7.5e-112
Identity = 260/491 (52.95%), Postives = 309/491 (62.93%), Query Frame = 0

Query: 111 QDQGKAVGAGRA--EFPMDADGYKILCQIGVSEHSVVYKAICESV-NSTTVAVKVVDMDG 170
           QD   A  AGR   +FP  ADGYKIL QIGVS+HSVVYKAICES  NS  VAVKV+D+D 
Sbjct: 8   QDPENAAAAGRLHFQFPTAADGYKILYQIGVSDHSVVYKAICESANNSAKVAVKVIDVDR 67

Query: 171 LDWDHVKNIINSLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPD 230
              +     +NSLSLI H NILSPHC F +DR+LW VMPFIH G L+ ++A RF NGLPD
Sbjct: 68  HVDEFFVMKMNSLSLIDHANILSPHCCFTVDRRLWTVMPFIHAGPLQAILANRFPNGLPD 127

Query: 231 QFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSS 290
           QFLAILLHQLL  L CLH+LGYVH +IK SNIF+DSEG+VKLSD+NV+       D+  S
Sbjct: 128 QFLAILLHQLLRSLICLHELGYVHRNIKASNIFVDSEGSVKLSDLNVY---VGDDDDDDS 187

Query: 291 SLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYR 350
           S+PYW  P     D EYS KSD+WAIGIVALE+  GC  +S+   +RSQ L ISRR PY 
Sbjct: 188 SVPYWDPP-----DEEYSFKSDVWAIGIVALEVAYGCPPISDSRTIRSQLLNISRRFPYN 247

Query: 351 LNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCF 410
           LN+    SK  ID KF   + FKDFVS CLVKDPS+R SA SLL HPFI + E S     
Sbjct: 248 LNLDKMSSKSVIDRKF--GELFKDFVSSCLVKDPSKRPSAASLLAHPFIKKGETSN---- 307

Query: 411 VSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVKIDRWDFNKSHFQLEP 470
           V  KSI KDM GIED FE NKDRL  LM   R+  DN   G +VK+  WDFN   F+LEP
Sbjct: 308 VLFKSIAKDMAGIEDRFEKNKDRLVELMKKDRRVEDN-NRGNVVKVSGWDFNNFLFELEP 367

Query: 471 IKEETLAEKV----GTII--------IAEEGK---------------------------- 530
             EE+L ++V     TII        + E G+                            
Sbjct: 368 -DEESLGKRVRFRGETIIPLRDDHVSLNEAGEASKFNVPLLLKRMDSLSLSGLSGRMGSR 427

Query: 531 -EEGGRRTVDEKL--VLSSLRESLNK-------VIAQFGLFFKRELEMTREIERLKAELG 549
            EE  R    E L  VL+SL ES+++       VI+QFG   +R++EMT+EI++LKAEL 
Sbjct: 428 AEEEVRVMDSETLAKVLTSLVESIDQQREVIMMVISQFGRLLERDMEMTKEIKKLKAELA 482

BLAST of Sgr025623 vs. ExPASy TrEMBL
Match: A0A6J1KYZ4 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111498878 PE=4 SV=1)

HSP 1 Score: 322.4 bits (825), Expect = 3.9e-84
Identity = 203/480 (42.29%), Postives = 293/480 (61.04%), Query Frame = 0

Query: 107 RGRVQDQGKAVGAG-RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNST-TVAVKVVD 166
           R ++QDQ  +   G   +FP  AD Y+I  QIG S+HS VYKAIC+S +ST  VA+K++D
Sbjct: 3   RRQIQDQETSAAKGTHFKFPKVADEYEIQSQIGASDHSTVYKAICKSADSTMIVAIKIID 62

Query: 167 MD-GLDWDHVKNIINSLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQ 226
            +  +D   + NI  +LS I HP+ILSPHC F+  D  LWIVMPFIH G L+ +++ RF+
Sbjct: 63  SNVEIDPTAITNINTNLSFINHPHILSPHCCFIANDGCLWIVMPFIHAGPLQAIISQRFR 122

Query: 227 NGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWAS 286
            GLPDQF++IL++QLL  L  LH+ G VH  +K SNIF D+ G V LSD++V K      
Sbjct: 123 TGLPDQFMSILINQLLRALVFLHEKGLVHKSVKASNIFFDTHGCVLLSDLDVSKD----M 182

Query: 287 DEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISR 346
           ++  SS+PYWV PE      +YSSKSD+W++GI+A+EI  GC  + +  ++++QF KISR
Sbjct: 183 NKDCSSVPYWVPPE-----EDYSSKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 242

Query: 347 RLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEES 406
           R PY LN+     K  +  KF   D+ K FV  CL KDP  R SA SLL HPF+ + E S
Sbjct: 243 RFPYDLNLDTRSGKSVVPRKF--GDTLKHFVGSCLAKDPFARVSALSLLDHPFVKKGENS 302

Query: 407 RNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKE---NGQMVKIDRWDFN 466
                   KSI K MVGI +MFE +KDR+  LM N  +  ++ +    G++++I  WD+N
Sbjct: 303 N----FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGGGKVLRITGWDYN 362

Query: 467 KSHFQLEPIKEETLAEKV---GTIIIAEEGKEE-----------GG---RRTVDEKL--- 526
              F+LEP  EE+  ++V   G  + + + +E+           GG   ++ VD+ +   
Sbjct: 363 NFLFELEP-DEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSDEKKEVDQAMDSE 422

Query: 527 ----VLSSL-------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERSFELEL 549
               +LSSL       +E ++ ++   G    ++LEM ++I+RL+ EL AEKE+SF+LEL
Sbjct: 423 TLATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Sgr025623 vs. ExPASy TrEMBL
Match: A0A6J1H6C5 (serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC111460018 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 3.1e-81
Identity = 195/479 (40.71%), Postives = 282/479 (58.87%), Query Frame = 0

Query: 107 RGRVQDQGKAVGAG-RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNS-TTVAVKVVD 166
           R ++QDQ  +   G  ++FP  AD Y+I  QIG S+HS VYKAIC+S ++ T VA+K++D
Sbjct: 3   RRQIQDQETSAAKGTHSKFPKVADEYEIQSQIGASDHSTVYKAICKSADTPTIVAIKIID 62

Query: 167 MD-GLDWDHVKNIINSLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQ 226
            +  +D   + NI  +LS I HP+IL+PHC F   D +LWIVMPFI  G L+ +++ RF+
Sbjct: 63  SNVEIDQTAITNINTNLSFINHPHILTPHCCFTAPDGRLWIVMPFIPAGPLQAIISQRFR 122

Query: 227 NGLPDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWAS 286
            GLPDQF++IL++QLL  L  LH+ G VH  +K SNIF D+ G V LSD++V K      
Sbjct: 123 TGLPDQFMSILINQLLRALVFLHEKGLVHKAVKASNIFFDTHGCVLLSDLDVSK----EM 182

Query: 287 DEYSSSLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISR 346
            +  SS+PYW  PE      +YS KSD+W++GI+A+EI  GC  + +  ++++QF KISR
Sbjct: 183 KKDCSSVPYWDPPE-----EDYSFKSDVWSVGILAMEIAYGCPPIYDQKVIKAQFSKISR 242

Query: 347 RLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEES 406
           R PY LN+ N   K  +  KF   D+ K FV  CL KD   R SA SLL HPF+ + E S
Sbjct: 243 RFPYDLNLDNRSGKSVVPRKF--GDTLKHFVGSCLAKDRFARASALSLLDHPFVKKGENS 302

Query: 407 RNNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKE-NGQMVKIDRWDFNKS 466
                   KSI K MVGI +MFE +KDR+  LM N  +  ++ +  G++++I  WD+N  
Sbjct: 303 N----FLFKSIAKGMVGIGEMFEQDKDRIIDLMKNEVQLGESSQGGGKVLRITGWDYNNF 362

Query: 467 HFQLEP------------------IKEETLAEKVGTIIIAEEGKEEGGRRTVDEKL---- 526
            F+LEP                   KE+    K     +   G  +  ++ VD+ +    
Sbjct: 363 LFELEPDEESFGKRVRFRGETVFSYKEQEQCNKFDMKSLMHGGSSDEKKKEVDQAMDTET 422

Query: 527 ---VLSSL-------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERSFELEL 549
              +LSSL       +E ++ ++   G    ++LEM ++I+RL+ EL AEKE+SF+LEL
Sbjct: 423 LATLLSSLLDNIDTQKEMVSMLMNHCGFMHDKDLEMVKQIKRLEDELAAEKEKSFQLEL 466

BLAST of Sgr025623 vs. ExPASy TrEMBL
Match: A0A5D3C8A9 (Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1503G00250 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 9.6e-67
Identity = 185/485 (38.14%), Postives = 270/485 (55.67%), Query Frame = 0

Query: 124 FPMDADGYKILCQIGVSEHSVVYKAICESVNST-----TVAVKVVDMDGLDWDHVKNIIN 183
           FP  +D Y IL QIG S +S VY A C+S +S+     TVA+K+++   + +D   N++N
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFD--TNLLN 62

Query: 184 -SLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQ 243
            SL+LI HPNI+ PHC F   D +LWIVMP++  G L+++++  F  G+PDQF +ILL Q
Sbjct: 63  TSLTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQ 122

Query: 244 LLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSSSLPYWVAPE 303
           +L  L  LH++G+VH  IKPSNIF DS+GTVKLS  ++        D   SS+PYWV P 
Sbjct: 123 VLQALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISADNKIDR-DSSIPYWVPP- 182

Query: 304 LGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLSK 363
               D EYS KSDIW +G++A+E+T G   + +  IL SQ L ISRR PY L       +
Sbjct: 183 ----DDEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETG-RR 242

Query: 364 DAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLKSIVKD 423
           ++ D +    D+ K+FV  CL+KD  ER +A SLL HPF+++ E + N  F   KSI K 
Sbjct: 243 NSGDRRI-FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLF---KSIPKG 302

Query: 424 MVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVKIDRWDFNKSHFQLEPIKEETLAEK 483
           ++ I+  F+  K+R+  +M N   E    ++ ++ KI  W +N   F+LEP   E+L ++
Sbjct: 303 VIEIDRRFQLEKERIIGIMNN--DEGLKNQSEKITKISGWVYNDFLFELEP-DGESLGKR 362

Query: 484 V--GTIIIAEEGKEE----------------------------GG-------------RR 543
           V  G   I E  KEE                            GG             ++
Sbjct: 363 VRFGGETIREFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKK 422

Query: 544 TVDEKLVLSSL----------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERS 549
            VD++ ++  L          RE ++ +I   G   +++ EM+ EI+RL+ EL AEKE+ 
Sbjct: 423 IVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQY 471

BLAST of Sgr025623 vs. ExPASy TrEMBL
Match: A0A1S3CN88 (serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC103502862 PE=4 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 9.6e-67
Identity = 185/485 (38.14%), Postives = 270/485 (55.67%), Query Frame = 0

Query: 124 FPMDADGYKILCQIGVSEHSVVYKAICESVNST-----TVAVKVVDMDGLDWDHVKNIIN 183
           FP  +D Y IL QIG S +S VY A C+S +S+     TVA+K+++   + +D   N++N
Sbjct: 3   FPTTSDQYTILTQIGASPNSAVYLAKCDSQDSSTSSTVTVAIKIIETPQIIFD--TNLLN 62

Query: 184 -SLSLIQHPNILSPHCSFMM-DRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQ 243
            SL+LI HPNI+ PHC F   D +LWIVMP++  G L+++++  F  G+PDQF +ILL Q
Sbjct: 63  TSLTLIDHPNIVRPHCCFFADDNRLWIVMPYMSAGPLQSVLSHSFPRGMPDQFTSILLSQ 122

Query: 244 LLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSSSLPYWVAPE 303
           +L  L  LH++G+VH  IKPSNIF DS+GTVKLS  ++        D   SS+PYWV P 
Sbjct: 123 VLQALVYLHRMGFVHKAIKPSNIFFDSQGTVKLSVFDLMISADNKIDR-DSSIPYWVPP- 182

Query: 304 LGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLSK 363
               D EYS KSDIW +G++A+E+T G   + +  IL SQ L ISRR PY L       +
Sbjct: 183 ----DDEYSFKSDIWEVGVLAMEVTWGGPPIIDPRILESQVLNISRRFPYELKTETG-RR 242

Query: 364 DAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLKSIVKD 423
           ++ D +    D+ K+FV  CL+KD  ER +A SLL HPF+++ E + N  F   KSI K 
Sbjct: 243 NSGDRRI-FGDAVKEFVKTCLLKDRLERPTAASLLDHPFVVKGENANNFLF---KSIPKG 302

Query: 424 MVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVKIDRWDFNKSHFQLEPIKEETLAEK 483
           ++ I+  F+  K+R+  +M N   E    ++ ++ KI  W +N   F+LEP   E+L ++
Sbjct: 303 VIEIDRRFQLEKERIIGIMNN--DEGLKNQSEKITKISGWVYNDFLFELEP-DGESLGKR 362

Query: 484 V--GTIIIAEEGKEE----------------------------GG-------------RR 543
           V  G   I E  KEE                            GG             ++
Sbjct: 363 VRFGGETIREFEKEEEEEEEGESSTKVDDTPSLLEIIEKNLMSGGDGSGESSKKKKKKKK 422

Query: 544 TVDEKLVLSSL----------RESLNKVIAQFGLFFKRELEMTREIERLKAELGAEKERS 549
            VD++ ++  L          RE ++ +I   G   +++ EM+ EI+RL+ EL AEKE+ 
Sbjct: 423 IVDQESLVKMLTSVLGNIDAQREMVSMLINHVGYLSEKQSEMSNEIKRLETELKAEKEQY 471

BLAST of Sgr025623 vs. TAIR 10
Match: AT4G14480.1 (Protein kinase superfamily protein )

HSP 1 Score: 238.4 bits (607), Expect = 1.4e-62
Identity = 163/487 (33.47%), Postives = 269/487 (55.24%), Query Frame = 0

Query: 121 RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMD--GLDWDHVKNIIN 180
           + EFP+DA+ Y+I+C+IGV   + VYKAIC  +NS  VA+K +D+D    D+D ++    
Sbjct: 5   KLEFPLDAEAYEIICKIGVGVSASVYKAICIPMNSMVVAIKAIDLDQSRADFDSLRRETK 64

Query: 181 SLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLL 240
           ++SL+ HPNIL+ +CSF +DR LW+VMPF+  GSL +++++ F +GLP+  +++ L + L
Sbjct: 65  TMSLLSHPNILNAYCSFTVDRCLWVVMPFMSCGSLHSIVSSSFPSGLPENCISVFLKETL 124

Query: 241 SGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSD----VNVFKPTTWASDEYSSSL----- 300
           + ++ LH  G++H DIK  NI +DS+G+VKL+D     ++++P T +S   SSSL     
Sbjct: 125 NAISYLHDQGHLHRDIKAGNILVDSDGSVKLADFGVSASIYEPVTSSSGTTSSSLRLTDI 184

Query: 301 ---PYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRL-- 360
              PYW+APE+  S   Y  K+DIW+ GI ALE+  G   +S+   L+S  +KI++R   
Sbjct: 185 AGTPYWMAPEVVHSHTGYGFKADIWSFGITALELAHGRPPLSHLPPLKSLLMKITKRFHF 244

Query: 361 -PYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESR 420
             Y +N      K   + KF  S +F++ V  CL +DP++R SAE LL HPF    +  +
Sbjct: 245 SDYEINTSGSSKKG--NKKF--SKAFREMVGLCLEQDPTKRPSAEKLLKHPFF---KNCK 304

Query: 421 NNCFVSLKSIVKDMVGIEDMFETNKDRLEFLMANGRKEIDNKENGQMVK---IDRWDFNK 480
              FV +K+++  +   E MF  ++  ++ +  +  +E   +E+ ++VK   I  W+F +
Sbjct: 305 GLDFV-VKNVLHSLSNAEQMFMESQILIKSVGDDDEEE--EEEDEEIVKNRRISGWNFRE 364

Query: 481 SHFQLEPI-------------KEETLAE-------------KVGTIIIAEEGK-EEGGRR 540
              QL P+             +EE  ++             ++G  +  EE K +EG   
Sbjct: 365 DDLQLSPVFPATESDSSESSPREEDQSKDKKEDDNVTITGYELGLGLSNEEAKNQEGEVV 424

Query: 541 TVDEKLVLSS---LRESLNKVIAQFGLFFK----------RELEMTREIERLKAELGAEK 548
             D+ LVL     L++SL    A+  +  +          RE E+   +E+LK EL  EK
Sbjct: 425 GFDKDLVLEKLKVLKKSLEHQRARVSIIIEALSGDKEEKSREEELLEMVEKLKIELETEK 481

BLAST of Sgr025623 vs. TAIR 10
Match: AT5G14720.1 (Protein kinase superfamily protein )

HSP 1 Score: 172.6 bits (436), Expect = 9.6e-43
Identity = 107/348 (30.75%), Postives = 176/348 (50.57%), Query Frame = 0

Query: 123 EFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMD--GLDWDHVKNIINSL 182
           +FP++A  YK+  +IG    + V++A+C  +N   VA+KV+D++    D D ++  + ++
Sbjct: 8   KFPLNAKDYKLYEEIGDGVSATVHRALCIPLN-VVVAIKVLDLEKCNNDLDGIRREVQTM 67

Query: 183 SLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLLSG 242
           SLI HPN+L  HCSF    QLW+VMP++  GS   ++ + + +G  +  +A LL + L  
Sbjct: 68  SLINHPNVLQAHCSFTTGHQLWVVMPYMAGGSCLHIIKSSYPDGFEEPVIATLLRETLKA 127

Query: 243 LACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSS-----SLPYWVAP 302
           L  LH  G++H D+K  NI +DS G VKL+D  V        D   S       P W+AP
Sbjct: 128 LVYLHAHGHIHRDVKAGNILLDSNGAVKLADFGVSACMFDTGDRQRSRNTFVGTPCWMAP 187

Query: 303 ELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNFLS 362
           E+      Y  K+D+W+ GI ALE+  G    S +  ++   + +    P          
Sbjct: 188 EVMQQLHGYDFKADVWSFGITALELAHGHAPFSKYPPMKVLLMTLQNAPP---------- 247

Query: 363 KDAIDWKFS--LSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLKSI 422
              +D++     S +FK+ V  CLVKDP +R ++E LL HPF      +       +K+I
Sbjct: 248 --GLDYERDKRFSKAFKEMVGTCLVKDPKKRPTSEKLLKHPFFKHARPAD----YLVKTI 307

Query: 423 VKDMVGIEDMFETNKDRLEFLMANGRKEIDNKENGQ--MVKIDRWDFN 460
           +  +  + D +   K +   L+   + E +   + Q  +  I  W+FN
Sbjct: 308 LNGLPPLGDRYRQIKSKEADLLMQNKSEYEAHLSQQEYIRGISAWNFN 338

BLAST of Sgr025623 vs. TAIR 10
Match: AT1G79640.1 (Protein kinase superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 5.8e-40
Identity = 104/352 (29.55%), Postives = 183/352 (51.99%), Query Frame = 0

Query: 121 RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDM--DGLDWDHVKNIIN 180
           + ++P+  + Y +   IG    ++V++A+C   +   VA+K++D   D  D +++     
Sbjct: 6   KKKYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNISREAQ 65

Query: 181 SLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLL 240
           ++ L+ HPN+L  HCSF+ D  LW++MP++  GS   ++ A + +G  +  +A +L + L
Sbjct: 66  TMMLVDHPNVLKSHCSFVSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREAL 125

Query: 241 SGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSS-----SLPYWV 300
            GL  LHQ G++H D+K  NI + + G VKL D  V      + D   +       P W+
Sbjct: 126 KGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWM 185

Query: 301 APELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNF 360
           APE+      Y  K+DIW+ GI  LE+  G    S +  ++   + +    P        
Sbjct: 186 APEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPP-------- 245

Query: 361 LSKDAIDWK--FSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLK 420
                +D++     S SFK  ++ CLVKDPS+R SA+ LL H F    +++R++ +++ K
Sbjct: 246 ----GLDYERDKKFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFF---KQARSSDYIARK 305

Query: 421 SI--VKDMVG-IEDMFETNKDRL-EFLMANGRKEIDNKENGQMVKIDRWDFN 460
            +  + D+V  ++ +    +D L +  MA+G KE +  +N     I  W+FN
Sbjct: 306 LLDGLPDLVNRVQAIKRKEEDMLAQEKMADGEKE-ELSQNEYKRGISGWNFN 340

BLAST of Sgr025623 vs. TAIR 10
Match: AT1G79640.2 (Protein kinase superfamily protein )

HSP 1 Score: 163.3 bits (412), Expect = 5.8e-40
Identity = 104/352 (29.55%), Postives = 183/352 (51.99%), Query Frame = 0

Query: 121 RAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDM--DGLDWDHVKNIIN 180
           + ++P+  + Y +   IG    ++V++A+C   +   VA+K++D   D  D +++     
Sbjct: 3   KKKYPIGPEHYTLYEFIGQGVSALVHRALCIPFDE-VVAIKILDFERDNCDLNNISREAQ 62

Query: 181 SLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGLPDQFLAILLHQLL 240
           ++ L+ HPN+L  HCSF+ D  LW++MP++  GS   ++ A + +G  +  +A +L + L
Sbjct: 63  TMMLVDHPNVLKSHCSFVSDHNLWVIMPYMSGGSCLHILKAAYPDGFEEAIIATILREAL 122

Query: 241 SGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEYSS-----SLPYWV 300
            GL  LHQ G++H D+K  NI + + G VKL D  V      + D   +       P W+
Sbjct: 123 KGLDYLHQHGHIHRDVKAGNILLGARGAVKLGDFGVSACLFDSGDRQRTRNTFVGTPCWM 182

Query: 301 APELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKISRRLPYRLNMYNF 360
           APE+      Y  K+DIW+ GI  LE+  G    S +  ++   + +    P        
Sbjct: 183 APEVMEQLHGYDFKADIWSFGITGLELAHGHAPFSKYPPMKVLLMTLQNAPP-------- 242

Query: 361 LSKDAIDWK--FSLSDSFKDFVSYCLVKDPSERGSAESLLGHPFIMEQEESRNNCFVSLK 420
                +D++     S SFK  ++ CLVKDPS+R SA+ LL H F    +++R++ +++ K
Sbjct: 243 ----GLDYERDKKFSRSFKQMIASCLVKDPSKRPSAKKLLKHSFF---KQARSSDYIARK 302

Query: 421 SI--VKDMVG-IEDMFETNKDRL-EFLMANGRKEIDNKENGQMVKIDRWDFN 460
            +  + D+V  ++ +    +D L +  MA+G KE +  +N     I  W+FN
Sbjct: 303 LLDGLPDLVNRVQAIKRKEEDMLAQEKMADGEKE-ELSQNEYKRGISGWNFN 337

BLAST of Sgr025623 vs. TAIR 10
Match: AT4G24100.1 (Protein kinase superfamily protein )

HSP 1 Score: 159.5 bits (402), Expect = 8.4e-39
Identity = 101/293 (34.47%), Postives = 149/293 (50.85%), Query Frame = 0

Query: 108 GRVQDQGKAVGAGRAEFPMDADGYKILCQIGVSEHSVVYKAICESVNSTTVAVKVVDMD- 167
           GR    G      +  F M+   YK++ +IG    +VVY+AI    N   VA+K +D+D 
Sbjct: 10  GRGGGSGSGSSKQQRGFSMNPKDYKLMEEIGHGASAVVYRAIYLPTNE-VVAIKCLDLDR 69

Query: 168 -GLDWDHVKNIINSLSLIQHPNILSPHCSFMMDRQLWIVMPFIHVGSLETLMAARFQNGL 227
              + D ++    ++SLI HPN++   CSF +D  LW+VMPF+  GS   LM   + +G 
Sbjct: 70  CNSNLDDIRRESQTMSLIDHPNVIKSFCSFSVDHSLWVVMPFMAQGSCLHLMKTAYSDGF 129

Query: 228 PDQFLAILLHQLLSGLACLHQLGYVHNDIKPSNIFIDSEGTVKLSDVNVFKPTTWASDEY 287
            +  +  +L + L  L  LH+ G++H D+K  NI +D  G +KL D  V        D  
Sbjct: 130 EESAICCVLKETLKALDYLHRQGHIHRDVKAGNILLDDNGEIKLGDFGVSACLFDNGDRQ 189

Query: 288 SS-----SLPYWVAPELGGSDGEYSSKSDIWAIGIVALEITSGCLLVSNFLILRSQFLKI 347
            +       P W+APE+      Y+SK+DIW+ GI ALE+  G    S +  ++   + I
Sbjct: 190 RARNTFVGTPCWMAPEVLQPGNGYNSKADIWSFGITALELAHGHAPFSKYPPMKVLLMTI 249

Query: 348 SRRLPYRLNMYNFLSKDAIDWKFSLSDSFKDFVSYCLVKDPSERGSAESLLGH 394
               P        L  D  D KF  S SFK+ V+ CLVKD ++R +AE LL H
Sbjct: 250 QNAPP-------GLDYDR-DKKF--SKSFKEMVAMCLVKDQTKRPTAEKLLKH 291

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022138626.11.6e-11152.95serine/threonine-protein kinase BLUS1-like [Momordica charantia][more]
XP_023006000.18.1e-8442.29serine/threonine-protein kinase BLUS1-like [Cucurbita maxima][more]
XP_023548299.14.9e-8141.03serine/threonine-protein kinase BLUS1-like [Cucurbita pepo subsp. pepo][more]
XP_022958864.16.4e-8140.71serine/threonine-protein kinase BLUS1-like [Cucurbita moschata][more]
KAG6574930.16.4e-8140.71Serine/threonine-protein kinase BLUS1, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
O233042.0e-6133.47Serine/threonine-protein kinase BLUS1 OS=Arabidopsis thaliana OX=3702 GN=BLUS1 P... [more]
Q551H42.3e-3331.36Serine/threonine-protein kinase fray2 OS=Dictyostelium discoideum OX=44689 GN=fr... [more]
Q54XL63.9e-3331.63Serine/threonine-protein kinase fray1 OS=Dictyostelium discoideum OX=44689 GN=fr... [more]
Q86IX19.7e-3231.33Serine/threonine-protein kinase dst1 OS=Dictyostelium discoideum OX=44689 GN=dst... [more]
P084582.0e-2930.09Sporulation-specific protein 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 /... [more]
Match NameE-valueIdentityDescription
A0A6J1CA927.5e-11252.95serine/threonine-protein kinase BLUS1-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A6J1KYZ43.9e-8442.29serine/threonine-protein kinase BLUS1-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1H6C53.1e-8140.71serine/threonine-protein kinase BLUS1-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5D3C8A99.6e-6738.14Serine/threonine-protein kinase BLUS1-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3CN889.6e-6738.14serine/threonine-protein kinase BLUS1-like OS=Cucumis melo OX=3656 GN=LOC1035028... [more]
Match NameE-valueIdentityDescription
AT4G14480.11.4e-6233.47Protein kinase superfamily protein [more]
AT5G14720.19.6e-4330.75Protein kinase superfamily protein [more]
AT1G79640.15.8e-4029.55Protein kinase superfamily protein [more]
AT1G79640.25.8e-4029.55Protein kinase superfamily protein [more]
AT4G24100.18.4e-3934.47Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 522..542
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 120..207
e-value: 6.5E-18
score: 66.6
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 211..447
e-value: 7.4E-38
score: 131.9
NoneNo IPR availablePIRSRPIRSR037568-2PIRSR037568-2coord: 184..321
e-value: 2.8E-14
score: 49.4
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 235..322
e-value: 3.2E-6
score: 24.2
NoneNo IPR availablePANTHERPTHR48014:SF7SERINE/THREONINE-PROTEIN KINASE BLUS1coord: 119..569
NoneNo IPR availablePANTHERPTHR48014SERINE/THREONINE-PROTEIN KINASE FRAY2coord: 119..569
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 131..396
e-value: 2.5E-42
score: 156.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 131..396
e-value: 1.3E-38
score: 132.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 131..396
score: 34.915939
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 249..261
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 128..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025623.1Sgr025623.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity