Sgr025541 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025541
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionprotein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic
Locationtig00007724: 1356303 .. 1359438 (-)
RNA-Seq ExpressionSgr025541
SyntenySgr025541
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCGGGAACGAGAGGAAGAAGGGAAGGGTATGATTGTCATTATCAGGCATTTTGCCTTATATTTTAATTTTTGGGTATTTTAACAAATTTGAAGTAGCATAAGGGCATTTAGCCCAATTACCCAATTGCGACTGGGAGGGATTGGAAACCGGACATCGATGGAGGATAGGGTGTGCTCTGCTTACTGACAACTGCTGTTCACAAGTGGTGGAGTTGGAAAGCTCCGACATGATGGATTGAATTTAGACTCTAGGGTTGTTTCTTTCGAGTTCCCAGGGAGGATGAAGAAGCTAAGATGGACAATGGACGGGCAAGGTTTTTGGGACCTGGATGTTTCAACACCTAGAACACTGGATGGGTCGGCCTCCCCGGTTCCTGATGACTTGCTTCCCCTGGGATTGTCCAGAGGCACCAGACTTTCCAGGGCCAAACAGATCGATTTCATGCAGCGCTTCATGGCTGCACCTTTTGTCCCCTCTTATGCTCCCTCCCATGGCTTTGCTCTACATCGCGTGTTTACGATCCCCTTTTGGGACTCCGGGTAACTGCATCTGCATCCCACTCCCTGTTTACTCTCTATTGTCCTTGGCTTTTTCCGCTTTCCCCTCCTCTCTTTTCTTTTATTCTATCTCGCTCCTACGCCCACACACACACTTGACTCTAGTATTGATTTTCCATCAAATCTGGCTTAAAAGTACTGCAGGTCTGCTACTTTTTTAGGTCAGTTCAATTTGCAGAAGTTCTTGGCCTCTTTTAAGAGATCTGGAGAGATGAATCAATCGGCGTCCTCATTGCTGCAAGGCATTGGAAGGCACCTCTGCCACCGATCTTTGTATGCCCTTGGTTTCTCTTCTGATTTCTTGTTAACTCCGGATGATACGCTGCTGATCAGCTTCGACGGATACGGCGACAATGAAATACTTCGTACAAAAGCAGTACTCCACCACAAGGCATCTATCTCCACTCTCTTCCTAGCCTATATGCCCGGCCTCTAATAGAAATTGTTGAATCTGTCTAGCATCTTGTATTTCTTCTGCATGCTTTTTTACGGCATCTCTTGATGTAGATTTTTATTTACTCGTTGCAGTTTCTACATCATGATCTAACAATGGAGGCACTTTCTCCAGGACTTTTTGTGGACAAATGTGGTAACTACTGGGATGTGCCTTCTTCATTAGTCATTGATCTAGGTTCTGCTGCTTCTGACTCAGGTCCAAGTTATCACTTGTCTATGCACCACAATACCGGGTCTCCCTCACAAGCTGGAAGTGAACAGACCGGTGCGGTTCCTTTCTGTCTACTTCCTGGTCTATCAGTCAAGGCTGCTTTTTCCTATAAGAAGGACTTCGAAATCTGGAGAAGCAACGCCAAGAAGTTAAAGATGGTGCAACCATATGACATTTTCCTATCAAATCCTCATGTTTCATTGTCAGGAATCATTGGTATGAATCCATACTGATTTTCTTTCGTTCATACTCCTGATTATATATAGCCCCTAAAACTTTTACTCTTTTTCAATTCCATCCCTAAAGTTTTTAAAGTTTTAATTTTATCCTCTAGATATCTAAAATTTAAAATGAAGTTCGATTGGAATTAAATAAAATTAAATAAAGGAATGGCAACGCTGTATTTGAAAATATTATTTTCTCAAGGTTGTCATCCTTTGAAGGTTAAATATTCACCTCACTCTATTTGATAAAAAAATCTAACCAGCCCTCCATGATAAAAAATTTCAACCTGGACCTAATTGAATAATTTTTTACGTAAGTAGGGTCGGTGTTTATAGAAAATAATATTTTTAAATGTAACGTGATTATATATTTATTCAATTTCAACCATGTTAACGGGACTTTTAAAATTTAACCTTATTTTTATTTGCAGTCTTTTGCGCATATGGCATTTGCGGATGCAATTGCCAACTTGGCTAATGAAATGCAAGGATTTAACAAACTCAGATACCGTCATGATTCATCCGAAACTTTTTTTAAAATTCGAAGTTTTCTTGTGCTGTAACGTATTAGACATGTGAACTTTTGTCTTGCATCAGCAAAAAGAAGAAAATAGCTTAAAATACTTTTGTTTATTATATAGTTTTGCAGATGGTATGAAGTTGCAAGCAATTCCTTGGAAATTTAATATTTTGCAATTTTTATTAATTTTTGCTTACAATCTCCGTTGATCTTAAAAGGTGCTGTGGCTACTACCTACTTTGGAGACAATTCCGTTAGATCAGCGGCACAAGACAGTCTTCAGGAATTTAAAGGACTTCATATGCAGACTTCCAGTATAAGATCTACTGTTTTAGCGGATGTATTTGCTTCCATTTCTTTTTCAGCTCAGTATGGGATGTTTCAAAGGCTATTTCTGGATCTCACCCGATTTTCTGCACGTATGGATTTCCATTCTGGCTCCAAGTTCATTTCTGGAGCCATGCTTTTGATAGAAGATCTTTCCAATTCCCGACAGCCAAGAACTGAATCAGTGAAAGCCACTTTGCCTAATGCTAGATTTTCTCTTCAGCAGCAGGTATGTTTGCCCTTCATTCAGCTGCTGAGTGTGTCGTCTAAATCAATTATAATAATATAAAAAGAAAATAAAACAAATCTTGCTATAAGAACATATCTTCTTCCTCCTATGATAATCCGTTCTGCTTTTTGGATCAGTGAAGTTCTGATTCATGCGAGTTTTGGAAATTCTTTGTTAAAATGTCTTCAAGTTGAACCCATAATCAATAATTTAAACTCTGAACACTAGAACTTTTCCATAGTTGGAGAAGTTTGAAAGAAAACTGATGATATATGTCTCAATTGAAGTGGGTGTGAGGCATCAACTCATGGAAAAACCTTCTTTATAACTTTGTATCAAGGCCATTGACTTGGAATTATGCATATTATGACTTGTTGTTTGGGACGGTTTCGTGAAACATTGGCCGCGCAGATCGCTGGACCCGTCAGCTTTAGAGCAGATTCAGGAGTTACAATAGATTTGAATAAACCAGCGTGGGGTATACAAGTGGAGGAGCCTACATTTGCCTTGGAATATGCGTTGCAGGTCCTTGGTTCGGCTAAAGCCGTCGCTTGGTACTCACCCAAGCACAGAGAATTTATGGTAGAGCTCCGTTTCTATGAGAACTGA

mRNA sequence

ATGCGGGAACGAGAGGAAGAAGGGAAGGACTCTAGGGTTGTTTCTTTCGAGTTCCCAGGGAGGATGAAGAAGCTAAGATGGACAATGGACGGGCAAGGTTTTTGGGACCTGGATGTTTCAACACCTAGAACACTGGATGGGTCGGCCTCCCCGGTTCCTGATGACTTGCTTCCCCTGGGATTGTCCAGAGGCACCAGACTTTCCAGGGCCAAACAGATCGATTTCATGCAGCGCTTCATGGCTGCACCTTTTGTCCCCTCTTATGCTCCCTCCCATGGCTTTGCTCTACATCGCGTGTTTACGATCCCCTTTTGGGACTCCGGGTCTGCTACTTTTTTAGGTCAGTTCAATTTGCAGAAGTTCTTGGCCTCTTTTAAGAGATCTGGAGAGATGAATCAATCGGCGTCCTCATTGCTGCAAGGCATTGGAAGGCACCTCTGCCACCGATCTTTGTATGCCCTTGGTTTCTCTTCTGATTTCTTGTTAACTCCGGATGATACGCTGCTGATCAGCTTCGACGGATACGGCGACAATGAAATACTTCGTACAAAAGCAGTACTCCACCACAAGTTTCTACATCATGATCTAACAATGGAGGCACTTTCTCCAGGACTTTTTGTGGACAAATGTGGTAACTACTGGGATGTGCCTTCTTCATTAGTCATTGATCTAGGTTCTGCTGCTTCTGACTCAGGTCCAAGTTATCACTTGTCTATGCACCACAATACCGGGTCTCCCTCACAAGCTGGAAGTGAACAGACCGGTGCGGTTCCTTTCTGTCTACTTCCTGGTCTATCAGTCAAGGCTGCTTTTTCCTATAAGAAGGACTTCGAAATCTGGAGAAGCAACGCCAAGAAGTTAAAGATGGTGCAACCATATGACATTTTCCTATCAAATCCTCATGTTTCATTGTCAGGAATCATTGGTGCTGTGGCTACTACCTACTTTGGAGACAATTCCGTTAGATCAGCGGCACAAGACAGTCTTCAGGAATTTAAAGGACTTCATATGCAGACTTCCAGTATAAGATCTACTGTTTTAGCGGATGTATTTGCTTCCATTTCTTTTTCAGCTCAGTATGGGATGTTTCAAAGGCTATTTCTGGATCTCACCCGATTTTCTGCACGTATGGATTTCCATTCTGGCTCCAAGTTCATTTCTGGAGCCATGCTTTTGATAGAAGATCTTTCCAATTCCCGACAGCCAAGAACTGAATCAGTGAAAGCCACTTTGCCTAATGCTAGATTTTCTCTTCAGCAGCAGATCGCTGGACCCGTCAGCTTTAGAGCAGATTCAGGAGTTACAATAGATTTGAATAAACCAGCGTGGGGTATACAAGTGGAGGAGCCTACATTTGCCTTGGAATATGCGTTGCAGGTCCTTGGTTCGGCTAAAGCCGTCGCTTGGTACTCACCCAAGCACAGAGAATTTATGGTAGAGCTCCGTTTCTATGAGAACTGA

Coding sequence (CDS)

ATGCGGGAACGAGAGGAAGAAGGGAAGGACTCTAGGGTTGTTTCTTTCGAGTTCCCAGGGAGGATGAAGAAGCTAAGATGGACAATGGACGGGCAAGGTTTTTGGGACCTGGATGTTTCAACACCTAGAACACTGGATGGGTCGGCCTCCCCGGTTCCTGATGACTTGCTTCCCCTGGGATTGTCCAGAGGCACCAGACTTTCCAGGGCCAAACAGATCGATTTCATGCAGCGCTTCATGGCTGCACCTTTTGTCCCCTCTTATGCTCCCTCCCATGGCTTTGCTCTACATCGCGTGTTTACGATCCCCTTTTGGGACTCCGGGTCTGCTACTTTTTTAGGTCAGTTCAATTTGCAGAAGTTCTTGGCCTCTTTTAAGAGATCTGGAGAGATGAATCAATCGGCGTCCTCATTGCTGCAAGGCATTGGAAGGCACCTCTGCCACCGATCTTTGTATGCCCTTGGTTTCTCTTCTGATTTCTTGTTAACTCCGGATGATACGCTGCTGATCAGCTTCGACGGATACGGCGACAATGAAATACTTCGTACAAAAGCAGTACTCCACCACAAGTTTCTACATCATGATCTAACAATGGAGGCACTTTCTCCAGGACTTTTTGTGGACAAATGTGGTAACTACTGGGATGTGCCTTCTTCATTAGTCATTGATCTAGGTTCTGCTGCTTCTGACTCAGGTCCAAGTTATCACTTGTCTATGCACCACAATACCGGGTCTCCCTCACAAGCTGGAAGTGAACAGACCGGTGCGGTTCCTTTCTGTCTACTTCCTGGTCTATCAGTCAAGGCTGCTTTTTCCTATAAGAAGGACTTCGAAATCTGGAGAAGCAACGCCAAGAAGTTAAAGATGGTGCAACCATATGACATTTTCCTATCAAATCCTCATGTTTCATTGTCAGGAATCATTGGTGCTGTGGCTACTACCTACTTTGGAGACAATTCCGTTAGATCAGCGGCACAAGACAGTCTTCAGGAATTTAAAGGACTTCATATGCAGACTTCCAGTATAAGATCTACTGTTTTAGCGGATGTATTTGCTTCCATTTCTTTTTCAGCTCAGTATGGGATGTTTCAAAGGCTATTTCTGGATCTCACCCGATTTTCTGCACGTATGGATTTCCATTCTGGCTCCAAGTTCATTTCTGGAGCCATGCTTTTGATAGAAGATCTTTCCAATTCCCGACAGCCAAGAACTGAATCAGTGAAAGCCACTTTGCCTAATGCTAGATTTTCTCTTCAGCAGCAGATCGCTGGACCCGTCAGCTTTAGAGCAGATTCAGGAGTTACAATAGATTTGAATAAACCAGCGTGGGGTATACAAGTGGAGGAGCCTACATTTGCCTTGGAATATGCGTTGCAGGTCCTTGGTTCGGCTAAAGCCGTCGCTTGGTACTCACCCAAGCACAGAGAATTTATGGTAGAGCTCCGTTTCTATGAGAACTGA

Protein sequence

MREREEEGKDSRVVSFEFPGRMKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDDLLPLGLSRGTRLSRAKQIDFMQRFMAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRSGEMNQSASSLLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLTMEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAVPFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFGDNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARMDFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTIDLNKPAWGIQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN
Homology
BLAST of Sgr025541 vs. NCBI nr
Match: XP_022158716.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Momordica charantia])

HSP 1 Score: 790.4 bits (2040), Expect = 8.7e-225
Identity = 400/469 (85.29%), Postives = 426/469 (90.83%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPD--DLLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTMDGQGFW+LDVSTP TLDG+ASPVP    LLPLGLSRG RLSRAKQIDFMQRF
Sbjct: 1   MKKLRWTMDGQGFWELDVSTPTTLDGAASPVPAHLHLLPLGLSRGARLSRAKQIDFMQRF 60

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRSGEMNQSASSLL 141
           MAAPFVPSYAPSHGF+L RVF  PF   GS T LGQFNLQKF++SF RSG M+ S SS L
Sbjct: 61  MAAPFVPSYAPSHGFSLQRVFPFPF--PGSPTLLGQFNLQKFISSFTRSGVMHHSPSSFL 120

Query: 142 QGIGRHLCHRSLYALGFSSDFLLTPD--DTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           Q IGRHLCH S YALG SSD  L PD  D+LLISFDGYG+N +LRTKA+LHHKFLHHDLT
Sbjct: 121 QAIGRHLCHPSFYALGVSSDISLNPDDSDSLLISFDGYGENGMLRTKALLHHKFLHHDLT 180

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVD+ GNYWDVPSSL+IDLGSAA DSGPSYHLSMHHN GSPSQ+G+E+TGAV
Sbjct: 181 MEALSPGLFVDESGNYWDVPSSLLIDLGSAAFDSGPSYHLSMHHNAGSPSQSGTEKTGAV 240

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS+KAAFS+K +F+IWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG
Sbjct: 241 PFCLLPGLSLKAAFSFKHNFQIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 300

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           DNSVRSAAQDSLQEFKGL+MQTS IRSTVLADVFASISFSAQYGMFQR FLDLTRFSA M
Sbjct: 301 DNSVRSAAQDSLQEFKGLNMQTSKIRSTVLADVFASISFSAQYGMFQRGFLDLTRFSASM 360

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKFISGA LLIEDLSNS +PRTE+VKA LP+ARFSLQQQIAGP+SFRADS VTID
Sbjct: 361 DFHSGSKFISGAKLLIEDLSNSGKPRTETVKAILPDARFSLQQQIAGPISFRADSRVTID 420

Query: 442 LNKPAWGIQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           LNK  WG+QVEEPTFALEYALQVLGSAKA+AWYSPK REFMVELRFYEN
Sbjct: 421 LNKAGWGMQVEEPTFALEYALQVLGSAKAIAWYSPKPREFMVELRFYEN 467

BLAST of Sgr025541 vs. NCBI nr
Match: XP_038875869.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Benincasa hispida] >XP_038875870.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 787.7 bits (2033), Expect = 5.6e-224
Identity = 395/469 (84.22%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPD--DLLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRW MDGQGFWDLDVSTPRTLDGSASPVP    LLPLGLSRG RLSRAKQIDFMQ F
Sbjct: 2   MKKLRWAMDGQGFWDLDVSTPRTLDGSASPVPSHLHLLPLGLSRGVRLSRAKQIDFMQSF 61

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS--GEMNQSASS 141
           MAAPFVPSY+PSHGF+L RVF+IPF DSGS T LGQFNLQKF++S K+S  G+M QS SS
Sbjct: 62  MAAPFVPSYSPSHGFSLQRVFSIPFSDSGSVTLLGQFNLQKFISSLKKSGVGDMGQSLSS 121

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
            LQ IGRHLCHRSLYALG SSD LL PDD+L+ISFDGYGDNEI+RTKAV HHKFLHHDLT
Sbjct: 122 FLQCIGRHLCHRSLYALGISSDILLPPDDSLMISFDGYGDNEIVRTKAVFHHKFLHHDLT 181

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEA SPGLFVDK G YWDVPS+LV+DLGSAAS+SG SYHLSMH NTGSPSQ+GSEQ  + 
Sbjct: 182 MEAFSPGLFVDKSGKYWDVPSALVVDLGSAASESGLSYHLSMHQNTGSPSQSGSEQARSS 241

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           P CLLPGLS KAAF++KK+ EIWRSNAKKLKMVQPYDIFLS PHVSLSGIIGAVATTYFG
Sbjct: 242 PLCLLPGLSAKAAFAFKKNLEIWRSNAKKLKMVQPYDIFLSTPHVSLSGIIGAVATTYFG 301

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           DNS+RSAAQDSL EFKGL++QTS IRSTV ADVFASISFSAQYGMFQR +LDLT FSARM
Sbjct: 302 DNSIRSAAQDSLPEFKGLYLQTSRIRSTVFADVFASISFSAQYGMFQRKYLDLTCFSARM 361

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SGAMLLI+DLSNSR PRTESV+ATLP+ARFSLQQQIAGPVSFRADSGV ID
Sbjct: 362 DFHSGSKFLSGAMLLIDDLSNSRHPRTESVRATLPSARFSLQQQIAGPVSFRADSGVAID 421

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYE 486
           LNK  WG + V+EPTFALEYALQVLGSAKA+AWYSPKHREFMVELRFYE
Sbjct: 422 LNKAGWGLLGVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYE 470

BLAST of Sgr025541 vs. NCBI nr
Match: XP_023548884.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548885.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548886.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023548887.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 784.6 bits (2025), Expect = 4.8e-223
Identity = 399/470 (84.89%), Postives = 422/470 (89.79%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDD--LLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTM+GQ FWDLDVSTPRTLDGSASPVP D  LLPLGLSRG RLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS--GEMNQSASS 141
           MAAPFVPSY PSHGF+L RVF+IPF DSGSAT LGQFN+QKF++S K+S  GEM QS SS
Sbjct: 62  MAAPFVPSYTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSISS 121

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           LLQGIGRHL  RSLYA G SSD LLTPDD LLISFDGYGD++ILRTKAVLHHKFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDILRTKAVLHHKFLHHDLT 181

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVDK G YWDVPSSLVIDLGSAA+DSG SYHLSMHHN GSPSQ+GSEQT   
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSAATDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS KAAF+ KK+ EIWRSNAKKLK VQPYDIFLSNPHVSLSGIIGAVAT+YFG
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLSNPHVSLSGIIGAVATSYFG 301

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D S  SAA+ SLQEFKGL+MQTS IRSTV ADVFASISFSAQYGMFQR FLDLTRFS R 
Sbjct: 302 DISAGSAAEGSLQEFKGLYMQTSRIRSTVFADVFASISFSAQYGMFQRNFLDLTRFSGRF 361

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SGAMLLIEDLSNS+ PRTESVKATLPNARFS QQQIAGPVSFRAD+GV ID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADTGVAID 421

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           L+K  WG +QVEEPTFALEYAL VLGSAKA+AWYSPK REFMVELRFYEN
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALHVLGSAKAIAWYSPKQREFMVELRFYEN 471

BLAST of Sgr025541 vs. NCBI nr
Match: XP_023006570.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita maxima] >XP_023006571.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita maxima] >XP_023006572.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 778.5 bits (2009), Expect = 3.4e-221
Identity = 396/470 (84.26%), Postives = 419/470 (89.15%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDD--LLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTM+GQ FWDLDVSTPRTLDGSASPVP D  LLPLGLSRG RLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS--GEMNQSASS 141
           MAAPFVPSY PSHGF+L RVF+IPF DSGSAT LGQFN+QKF++S K+S  GEM QS SS
Sbjct: 62  MAAPFVPSYTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSLSS 121

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           LLQGIGRHL  RSLYA G SSD LLTPDD LLISFDGYGD+++LRTKAVLHHKFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDVLRTKAVLHHKFLHHDLT 181

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVDK G YWDVPSSLVIDLGSAA+DSG SYHLSMHHN GSPSQ+GSEQT   
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSAATDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS KAAF+ KK+ EIWRSNAKKLK VQPYDIFLSNPHVSLSGIIGAVAT+YF 
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLSNPHVSLSGIIGAVATSYFR 301

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D SV SAA+ SLQEFKGLHMQTS IRSTV ADVFASISFSAQYGMFQ  FLDLTRFS R 
Sbjct: 302 DISVGSAAEGSLQEFKGLHMQTSRIRSTVFADVFASISFSAQYGMFQSNFLDLTRFSGRF 361

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SGAMLLIEDLSNS+ PRTESVKATLPNARFS QQQIAGPVSFRAD+GV ID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADTGVAID 421

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           L+K  WG +QVEEPTFALEYAL  LGSAKA+AWYSPK  EFMVELRFYEN
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALYALGSAKAIAWYSPKQTEFMVELRFYEN 471

BLAST of Sgr025541 vs. NCBI nr
Match: XP_022959177.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita moschata] >XP_022959178.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita moschata] >XP_022959179.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita moschata] >XP_022959180.1 protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 777.7 bits (2007), Expect = 5.8e-221
Identity = 395/470 (84.04%), Postives = 420/470 (89.36%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDD--LLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTM+GQ FWDLDVSTPRTLDGSASPVP D  LLPLGLSRG RLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS--GEMNQSASS 141
           MAAPFVPS+ PSHGF+L RVF+IPF DSGSAT LGQFN+QKF++S K+S  GEM QS SS
Sbjct: 62  MAAPFVPSFTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSISS 121

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           LLQGIGRHL  RSLYA G SSD LLTPDD LLISFDGYGD++ILRTKAVLHHKFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDILRTKAVLHHKFLHHDLT 181

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVDK G YWDVPSSLVIDLGSA +DSG SYHLSMHHN GSPSQ+GSEQT   
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSADTDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS KAAF+ KK+ EIWRSNAKKLK VQPYDIFL+NPHVSLSGIIGAVAT+YFG
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLANPHVSLSGIIGAVATSYFG 301

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D S  SAA+ SLQEF+GL+MQTS IRSTV ADVFASISFSAQYGMFQR FLDLTRFS R 
Sbjct: 302 DISAGSAAEGSLQEFRGLYMQTSRIRSTVFADVFASISFSAQYGMFQRNFLDLTRFSGRF 361

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SGAMLLIEDLSNS+ PRTESVKATLPNARFS QQQIAGPVSFRADSGV ID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADSGVAID 421

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           L+K  WG +QVEEPTFALEYAL  LGSAKA+AWYSPK REFMVELRFYEN
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALYALGSAKAIAWYSPKQREFMVELRFYEN 471

BLAST of Sgr025541 vs. ExPASy Swiss-Prot
Match: Q9M903 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TGD4 PE=1 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 8.1e-133
Identity = 244/479 (50.94%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDDLLPLGLSRGTRLSRAKQIDFMQRFMA 81
           M ++RW  +G   WDLD+STP TL+G+A  VPDD LPLGLSRGTRLSR KQ++F  RFMA
Sbjct: 1   MNRMRWVGEGD-IWDLDMSTPVTLEGTARAVPDDPLPLGLSRGTRLSRPKQVEFFHRFMA 60

Query: 82  APFVPSYAP---------SHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRSGEMN 141
           +P +PS++P           GF+L RV T+PF ++   + LGQF++Q+F+    ++    
Sbjct: 61  SPLIPSFSPIRPNTGDGGGGGFSLQRVLTLPFSNNWLVSLLGQFDVQRFVTEIDKTKAFG 120

Query: 142 QSASSL----LQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGY-GD-NEILRTKAV 201
           + +SS     L  IG+HL  +SLYALGF S+FLL+PDDTLL+S+D Y GD ++  R KA+
Sbjct: 121 RGSSSTVASRLNTIGKHLKDKSLYALGFCSEFLLSPDDTLLLSYDAYKGDLDKNPRAKAI 180

Query: 202 LHHKFLHHDLTMEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSP 261
            +H+F  H+LT EA+ PGLFVDK G YWDVP S+ IDL S  ++SGPSYHL +HHN+GSP
Sbjct: 181 FNHEFPLHNLTAEAVWPGLFVDKHGEYWDVPLSMAIDLASLPAESGPSYHLCLHHNSGSP 240

Query: 262 SQAGSEQTGAVPFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSG 321
            +  S+     P  LLPGLS+K+A SY+ + ++WR    KL+  +PYD+FLS+PHV++SG
Sbjct: 241 KKLHSDTMEVPPPSLLPGLSLKSAVSYRTNMDLWRGTTPKLETCKPYDVFLSSPHVAVSG 300

Query: 322 IIGAVATTYFGDNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRL 381
           IIG+V T  FG+NS+RS  ++  +   G  +   S+ S  +AD     S +AQYG FQ+ 
Sbjct: 301 IIGSVMTAAFGENSIRSKFENDSEGVGGFSLHFPSVNSGFMADALGRASLTAQYGNFQKF 360

Query: 382 FLDLTRFSARMDFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPV 441
           F DLTRF AR+DF  G +F++GA  + +DL NSRQP  E+ +   P    SLQQQI GP 
Sbjct: 361 FFDLTRFHARLDFPHGLRFLTGATSVAQDLLNSRQPSLEAFQKICPEVLVSLQQQIVGPF 420

Query: 442 SFRADSGVTIDLNKPAWGIQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYE 486
           SF+ +SG+ IDL   A  + V++  FA+EYALQVL SAKAV  YSPK  EFMVELRF+E
Sbjct: 421 SFKVESGIEIDLRNGANPVTVDKTVFAIEYALQVLLSAKAVVSYSPKQNEFMVELRFFE 478

BLAST of Sgr025541 vs. ExPASy TrEMBL
Match: A0A6J1E1S3 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111025174 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 4.2e-225
Identity = 400/469 (85.29%), Postives = 426/469 (90.83%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPD--DLLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTMDGQGFW+LDVSTP TLDG+ASPVP    LLPLGLSRG RLSRAKQIDFMQRF
Sbjct: 1   MKKLRWTMDGQGFWELDVSTPTTLDGAASPVPAHLHLLPLGLSRGARLSRAKQIDFMQRF 60

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRSGEMNQSASSLL 141
           MAAPFVPSYAPSHGF+L RVF  PF   GS T LGQFNLQKF++SF RSG M+ S SS L
Sbjct: 61  MAAPFVPSYAPSHGFSLQRVFPFPF--PGSPTLLGQFNLQKFISSFTRSGVMHHSPSSFL 120

Query: 142 QGIGRHLCHRSLYALGFSSDFLLTPD--DTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           Q IGRHLCH S YALG SSD  L PD  D+LLISFDGYG+N +LRTKA+LHHKFLHHDLT
Sbjct: 121 QAIGRHLCHPSFYALGVSSDISLNPDDSDSLLISFDGYGENGMLRTKALLHHKFLHHDLT 180

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVD+ GNYWDVPSSL+IDLGSAA DSGPSYHLSMHHN GSPSQ+G+E+TGAV
Sbjct: 181 MEALSPGLFVDESGNYWDVPSSLLIDLGSAAFDSGPSYHLSMHHNAGSPSQSGTEKTGAV 240

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS+KAAFS+K +F+IWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG
Sbjct: 241 PFCLLPGLSLKAAFSFKHNFQIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 300

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           DNSVRSAAQDSLQEFKGL+MQTS IRSTVLADVFASISFSAQYGMFQR FLDLTRFSA M
Sbjct: 301 DNSVRSAAQDSLQEFKGLNMQTSKIRSTVLADVFASISFSAQYGMFQRGFLDLTRFSASM 360

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKFISGA LLIEDLSNS +PRTE+VKA LP+ARFSLQQQIAGP+SFRADS VTID
Sbjct: 361 DFHSGSKFISGAKLLIEDLSNSGKPRTETVKAILPDARFSLQQQIAGPISFRADSRVTID 420

Query: 442 LNKPAWGIQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           LNK  WG+QVEEPTFALEYALQVLGSAKA+AWYSPK REFMVELRFYEN
Sbjct: 421 LNKAGWGMQVEEPTFALEYALQVLGSAKAIAWYSPKPREFMVELRFYEN 467

BLAST of Sgr025541 vs. ExPASy TrEMBL
Match: A0A6J1KW75 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111499258 PE=4 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.7e-221
Identity = 396/470 (84.26%), Postives = 419/470 (89.15%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDD--LLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTM+GQ FWDLDVSTPRTLDGSASPVP D  LLPLGLSRG RLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS--GEMNQSASS 141
           MAAPFVPSY PSHGF+L RVF+IPF DSGSAT LGQFN+QKF++S K+S  GEM QS SS
Sbjct: 62  MAAPFVPSYTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSLSS 121

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           LLQGIGRHL  RSLYA G SSD LLTPDD LLISFDGYGD+++LRTKAVLHHKFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDVLRTKAVLHHKFLHHDLT 181

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVDK G YWDVPSSLVIDLGSAA+DSG SYHLSMHHN GSPSQ+GSEQT   
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSAATDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS KAAF+ KK+ EIWRSNAKKLK VQPYDIFLSNPHVSLSGIIGAVAT+YF 
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLSNPHVSLSGIIGAVATSYFR 301

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D SV SAA+ SLQEFKGLHMQTS IRSTV ADVFASISFSAQYGMFQ  FLDLTRFS R 
Sbjct: 302 DISVGSAAEGSLQEFKGLHMQTSRIRSTVFADVFASISFSAQYGMFQSNFLDLTRFSGRF 361

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SGAMLLIEDLSNS+ PRTESVKATLPNARFS QQQIAGPVSFRAD+GV ID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADTGVAID 421

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           L+K  WG +QVEEPTFALEYAL  LGSAKA+AWYSPK  EFMVELRFYEN
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALYALGSAKAIAWYSPKQTEFMVELRFYEN 471

BLAST of Sgr025541 vs. ExPASy TrEMBL
Match: A0A6J1H3U0 (protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111460245 PE=4 SV=1)

HSP 1 Score: 777.7 bits (2007), Expect = 2.8e-221
Identity = 395/470 (84.04%), Postives = 420/470 (89.36%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDD--LLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRWTM+GQ FWDLDVSTPRTLDGSASPVP D  LLPLGLSRG RLSRAKQIDFMQ+F
Sbjct: 2   MKKLRWTMEGQSFWDLDVSTPRTLDGSASPVPTDLQLLPLGLSRGVRLSRAKQIDFMQQF 61

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS--GEMNQSASS 141
           MAAPFVPS+ PSHGF+L RVF+IPF DSGSAT LGQFN+QKF++S K+S  GEM QS SS
Sbjct: 62  MAAPFVPSFTPSHGFSLQRVFSIPFSDSGSATLLGQFNVQKFVSSLKKSGFGEMGQSISS 121

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           LLQGIGRHL  RSLYA G SSD LLTPDD LLISFDGYGD++ILRTKAVLHHKFLHHDLT
Sbjct: 122 LLQGIGRHLRDRSLYAFGISSDILLTPDDALLISFDGYGDSDILRTKAVLHHKFLHHDLT 181

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLFVDK G YWDVPSSLVIDLGSA +DSG SYHLSMHHN GSPSQ+GSEQT   
Sbjct: 182 MEALSPGLFVDKSGKYWDVPSSLVIDLGSADTDSGLSYHLSMHHNAGSPSQSGSEQTCMA 241

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS KAAF+ KK+ EIWRSNAKKLK VQPYDIFL+NPHVSLSGIIGAVAT+YFG
Sbjct: 242 PFCLLPGLSAKAAFALKKNLEIWRSNAKKLKRVQPYDIFLANPHVSLSGIIGAVATSYFG 301

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D S  SAA+ SLQEF+GL+MQTS IRSTV ADVFASISFSAQYGMFQR FLDLTRFS R 
Sbjct: 302 DISAGSAAEGSLQEFRGLYMQTSRIRSTVFADVFASISFSAQYGMFQRNFLDLTRFSGRF 361

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SGAMLLIEDLSNS+ PRTESVKATLPNARFS QQQIAGPVSFRADSGV ID
Sbjct: 362 DFHSGSKFLSGAMLLIEDLSNSQHPRTESVKATLPNARFSFQQQIAGPVSFRADSGVAID 421

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYEN 487
           L+K  WG +QVEEPTFALEYAL  LGSAKA+AWYSPK REFMVELRFYEN
Sbjct: 422 LSKAGWGSLQVEEPTFALEYALYALGSAKAIAWYSPKQREFMVELRFYEN 471

BLAST of Sgr025541 vs. ExPASy TrEMBL
Match: A0A0A0K824 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G001740 PE=4 SV=1)

HSP 1 Score: 751.1 bits (1938), Expect = 2.8e-213
Identity = 375/469 (79.96%), Postives = 416/469 (88.70%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPD--DLLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRW MDGQGFWDLDVST RTLDGSASPVP    LLPLGLSRG RLSRAKQIDFMQRF
Sbjct: 1   MKKLRWAMDGQGFWDLDVSTSRTLDGSASPVPSPLHLLPLGLSRGVRLSRAKQIDFMQRF 60

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKR--SGEMNQSASS 141
           MAAPFVPSY+PSHGF+L RVF++PF DSGS T LGQFNLQKF++S  +  SGEM QS SS
Sbjct: 61  MAAPFVPSYSPSHGFSLQRVFSVPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMCQSYSS 120

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
           LLQ IGRHL  RSLYA+G S+D LL PDD+L+ISFDGYGD++I+RTKAV H KFLHHDLT
Sbjct: 121 LLQYIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 180

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           +EALSPGLF++KCG YWDVPSSLV+DLGS ASDSG SYHLSMH N G PSQ GSE T + 
Sbjct: 181 VEALSPGLFMEKCGRYWDVPSSLVVDLGSVASDSGLSYHLSMHQNAGFPSQLGSEPTHSA 240

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCLLPGLS KAAF++KK+FEIWRSNAKKLKMVQPYDIFLS PHVSLS IIGAVAT+YFG
Sbjct: 241 PFCLLPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 300

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D+  RSAAQDSL++FKG +M++S IRSTV AD+F SISFSAQYGMFQ+ +LDLTRFSA M
Sbjct: 301 DDLARSAAQDSLEKFKGFYMKSSRIRSTVFADLFTSISFSAQYGMFQKKYLDLTRFSACM 360

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SG+MLLI+DLSNSR P+TESVKATLPNARFS+QQQIAGPVSFRAD+GV ID
Sbjct: 361 DFHSGSKFLSGSMLLIDDLSNSRHPKTESVKATLPNARFSIQQQIAGPVSFRADTGVAID 420

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYE 486
           LNK  W  ++VEEPTFALEYAL VLGSAKA+AWYSPKHREFMVELRFYE
Sbjct: 421 LNKAGWDLLRVEEPTFALEYALHVLGSAKAIAWYSPKHREFMVELRFYE 469

BLAST of Sgr025541 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 738.8 bits (1906), Expect = 1.5e-209
Identity = 373/469 (79.53%), Postives = 410/469 (87.42%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPD--DLLPLGLSRGTRLSRAKQIDFMQRF 81
           MKKLRW MD  GFWDLDVST RTLDGSASPVP    LLPLGLSRG RLSRAKQIDFMQ F
Sbjct: 332 MKKLRWAMD--GFWDLDVSTSRTLDGSASPVPSPFHLLPLGLSRGVRLSRAKQIDFMQSF 391

Query: 82  MAAPFVPSYAPSHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKR--SGEMNQSASS 141
           M APFVPSY+PSHGF+L RVF+IPF DSGS T LGQFNLQKF++S  +  SGEM QS SS
Sbjct: 392 MVAPFVPSYSPSHGFSLQRVFSIPFSDSGSITLLGQFNLQKFMSSLMKTGSGEMGQSFSS 451

Query: 142 LLQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLT 201
            +Q IGRHL  RSLYA+G S+D LL PDD+L+ISFDGYGD++I+RTKAV H KFLHHDLT
Sbjct: 452 FIQCIGRHLYQRSLYAVGISADILLPPDDSLMISFDGYGDSDIVRTKAVFHRKFLHHDLT 511

Query: 202 MEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSPSQAGSEQTGAV 261
           MEALSPGLF+DK G YWDVPSSLV+DLGSAASDSG SYHLSMH NTG PS  GSE T + 
Sbjct: 512 MEALSPGLFMDKSGRYWDVPSSLVVDLGSAASDSGLSYHLSMHQNTGFPSPLGSEPTHSA 571

Query: 262 PFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 321
           PFCL PGLS KAAF++KK+FEIWRSNAKKLKMVQPYDIFLS PHVSLS IIGAVAT+YFG
Sbjct: 572 PFCLFPGLSAKAAFAFKKNFEIWRSNAKKLKMVQPYDIFLSTPHVSLSAIIGAVATSYFG 631

Query: 322 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 381
           D+ VRSAAQ SL EFKG +MQTS IRST+ AD+F SISFSAQYGMFQ+ +LDLTRFSA M
Sbjct: 632 DDLVRSAAQGSLAEFKGFYMQTSRIRSTIFADLFTSISFSAQYGMFQKKYLDLTRFSACM 691

Query: 382 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 441
           DFHSGSKF+SG+MLLI+DLSNSR P+TE+VKATLPNARFS+QQQIAGPVSFRADSGV ID
Sbjct: 692 DFHSGSKFLSGSMLLIDDLSNSRHPKTEAVKATLPNARFSIQQQIAGPVSFRADSGVAID 751

Query: 442 LNKPAWG-IQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYE 486
           LNK  W  ++V+EPTFALEYALQVLGSAKA+AWYSPKHREFMVELRFYE
Sbjct: 752 LNKAGWDLLRVDEPTFALEYALQVLGSAKAIAWYSPKHREFMVELRFYE 798

BLAST of Sgr025541 vs. TAIR 10
Match: AT3G06960.1 (pigment defective 320 )

HSP 1 Score: 475.3 bits (1222), Expect = 5.8e-134
Identity = 244/479 (50.94%), Postives = 328/479 (68.48%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDDLLPLGLSRGTRLSRAKQIDFMQRFMA 81
           M ++RW  +G   WDLD+STP TL+G+A  VPDD LPLGLSRGTRLSR KQ++F  RFMA
Sbjct: 1   MNRMRWVGEGD-IWDLDMSTPVTLEGTARAVPDDPLPLGLSRGTRLSRPKQVEFFHRFMA 60

Query: 82  APFVPSYAP---------SHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRSGEMN 141
           +P +PS++P           GF+L RV T+PF ++   + LGQF++Q+F+    ++    
Sbjct: 61  SPLIPSFSPIRPNTGDGGGGGFSLQRVLTLPFSNNWLVSLLGQFDVQRFVTEIDKTKAFG 120

Query: 142 QSASSL----LQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGY-GD-NEILRTKAV 201
           + +SS     L  IG+HL  +SLYALGF S+FLL+PDDTLL+S+D Y GD ++  R KA+
Sbjct: 121 RGSSSTVASRLNTIGKHLKDKSLYALGFCSEFLLSPDDTLLLSYDAYKGDLDKNPRAKAI 180

Query: 202 LHHKFLHHDLTMEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSP 261
            +H+F  H+LT EA+ PGLFVDK G YWDVP S+ IDL S  ++SGPSYHL +HHN+GSP
Sbjct: 181 FNHEFPLHNLTAEAVWPGLFVDKHGEYWDVPLSMAIDLASLPAESGPSYHLCLHHNSGSP 240

Query: 262 SQAGSEQTGAVPFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSG 321
            +  S+     P  LLPGLS+K+A SY+ + ++WR    KL+  +PYD+FLS+PHV++SG
Sbjct: 241 KKLHSDTMEVPPPSLLPGLSLKSAVSYRTNMDLWRGTTPKLETCKPYDVFLSSPHVAVSG 300

Query: 322 IIGAVATTYFGDNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRL 381
           IIG+V T  FG+NS+RS  ++  +   G  +   S+ S  +AD     S +AQYG FQ+ 
Sbjct: 301 IIGSVMTAAFGENSIRSKFENDSEGVGGFSLHFPSVNSGFMADALGRASLTAQYGNFQKF 360

Query: 382 FLDLTRFSARMDFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPV 441
           F DLTRF AR+DF  G +F++GA  + +DL NSRQP  E+ +   P    SLQQQI GP 
Sbjct: 361 FFDLTRFHARLDFPHGLRFLTGATSVAQDLLNSRQPSLEAFQKICPEVLVSLQQQIVGPF 420

Query: 442 SFRADSGVTIDLNKPAWGIQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYE 486
           SF+ +SG+ IDL   A  + V++  FA+EYALQVL SAKAV  YSPK  EFMVELRF+E
Sbjct: 421 SFKVESGIEIDLRNGANPVTVDKTVFAIEYALQVLLSAKAVVSYSPKQNEFMVELRFFE 478

BLAST of Sgr025541 vs. TAIR 10
Match: AT3G06960.2 (pigment defective 320 )

HSP 1 Score: 312.8 bits (800), Expect = 5.0e-85
Identity = 157/306 (51.31%), Postives = 216/306 (70.59%), Query Frame = 0

Query: 22  MKKLRWTMDGQGFWDLDVSTPRTLDGSASPVPDDLLPLGLSRGTRLSRAKQIDFMQRFMA 81
           M ++RW  +G   WDLD+STP TL+G+A  VPDD LPLGLSRGTRLSR KQ++F  RFMA
Sbjct: 1   MNRMRWVGEGD-IWDLDMSTPVTLEGTARAVPDDPLPLGLSRGTRLSRPKQVEFFHRFMA 60

Query: 82  APFVPSYAP---------SHGFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRSGEMN 141
           +P +PS++P           GF+L RV T+PF ++   + LGQF++Q+F+    ++    
Sbjct: 61  SPLIPSFSPIRPNTGDGGGGGFSLQRVLTLPFSNNWLVSLLGQFDVQRFVTEIDKTKAFG 120

Query: 142 QSASSL----LQGIGRHLCHRSLYALGFSSDFLLTPDDTLLISFDGY-GD-NEILRTKAV 201
           + +SS     L  IG+HL  +SLYALGF S+FLL+PDDTLL+S+D Y GD ++  R KA+
Sbjct: 121 RGSSSTVASRLNTIGKHLKDKSLYALGFCSEFLLSPDDTLLLSYDAYKGDLDKNPRAKAI 180

Query: 202 LHHKFLHHDLTMEALSPGLFVDKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSP 261
            +H+F  H+LT EA+ PGLFVDK G YWDVP S+ IDL S  ++SGPSYHL +HHN+GSP
Sbjct: 181 FNHEFPLHNLTAEAVWPGLFVDKHGEYWDVPLSMAIDLASLPAESGPSYHLCLHHNSGSP 240

Query: 262 SQAGSEQTGAVPFCLLPGLSVKAAFSYKKDFEIWRSNAKKLKMVQPYDIFLSNPHVSLSG 313
            +  S+     P  LLPGLS+K+A SY+ + ++WR    KL+  +PYD+FLS+PHV++SG
Sbjct: 241 KKLHSDTMEVPPPSLLPGLSLKSAVSYRTNMDLWRGTTPKLETCKPYDVFLSSPHVAVSG 300

BLAST of Sgr025541 vs. TAIR 10
Match: AT2G44640.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion, chloroplast, plasma membrane, plastid, chloroplast envelope; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3769 (InterPro:IPR022244); BEST Arabidopsis thaliana protein match is: pigment defective 320 (TAIR:AT3G06960.1); Has 49 Blast hits to 48 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 48; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 244.6 bits (623), Expect = 1.7e-64
Identity = 151/468 (32.26%), Postives = 237/468 (50.64%), Query Frame = 0

Query: 34  FWDLDVSTPRTLDGSASPVPDDLLPLGLSRGTRLSRAKQIDFMQRFMAAPFVPSYAPSH- 93
           FWD +VS+P+TL+G+A  VP +  PL  +R +R  R +Q+  ++       +PS AP+  
Sbjct: 12  FWDQNVSSPQTLEGTARSVPGEPFPLDGARASRSHRIQQLSLLREGFPLGIIPSLAPASD 71

Query: 94  ----GFALHRVFTIPFWDSGSATFLGQFNLQKFLASFKRS-GEMNQSASSLLQGIGRHLC 153
                F+L+ +   P  ++     +GQF  +K  A  K       +    +++   +H+ 
Sbjct: 72  KRLGSFSLNSLLLSPSSNNWWLGLVGQFKPKKLFADIKADISNAEEWDLQVVKDTAKHIV 131

Query: 154 HRSLYALGFSSDFLLTPDDTLLISFDGYGDNEILRTKAVLHHKFLHHDLTMEALSPGLFV 213
            +SLY++G  +   L    +LL+S +  GD   LR K +L H    HDLT+EA  P LF+
Sbjct: 132 DKSLYSIGLWTQIALGTSSSLLLSTERLGDKNGLRNKLMLVHPLEKHDLTVEAAWPDLFL 191

Query: 214 DKCGNYWDVPSSLVIDLGSAASDSGPSYHLSMHHNTGSP---SQAGSEQTGAVPFCLLPG 273
           D  G +WDVP SL +D+ S   +SG  Y   +H + G+P   + AG E     P  L+PG
Sbjct: 192 DNKGRFWDVPESLNVDVSSLVPESGVRYRFGLHKSRGNPQPVNAAGVESGSDAPTSLMPG 251

Query: 274 LSVKAAFSYKKDFEIWRSNAKK-------LKMVQPYDIFLSNPHVSLSGIIGAVATTYFG 333
           L  KAA SYK + ++WR   K+         +  PYD+ L  PH ++SGI+G+    +  
Sbjct: 252 LCAKAAVSYKVNRDLWRPQEKEGNTEEEDKPVFLPYDLRLKEPHAAISGIVGSSLAAWIT 311

Query: 334 DNSVRSAAQDSLQEFKGLHMQTSSIRSTVLADVFASISFSAQYGMFQRLFLDLTRFSARM 393
              +               +     RS + ADVF S  ++ Q G F +L+ DLTR  AR+
Sbjct: 312 GRGM---------------LVNGKKRSPISADVFGSACYTFQKGRFSKLYGDLTRVDARV 371

Query: 394 DFHSGSKFISGAMLLIEDLSNSRQPRTESVKATLPNARFSLQQQIAGPVSFRADSGVTID 453
           D  S       A  L + L ++    ++    + P      QQQ+AGP+ F+ DS   + 
Sbjct: 372 DLPS-------AFALAKKLFHASSNNSDDTLWS-PRLNLIFQQQVAGPIVFKVDSQFQVG 431

Query: 454 LNKPAWGIQVEEPTFALEYALQVLGSAKAVAWYSPKHREFMVELRFYE 486
                   ++E+  ++L Y+L++L S K VAWYSPK +E M+ELR +E
Sbjct: 432 ------AARMEDVIYSLNYSLRLLESGKIVAWYSPKRKEGMIELRVFE 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022158716.18.7e-22585.29protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Momordica charantia][more]
XP_038875869.15.6e-22484.22protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Benincasa hispida] >XP_038... [more]
XP_023548884.14.8e-22384.89protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita pepo subsp. pepo... [more]
XP_023006570.13.4e-22184.26protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita maxima] >XP_0230... [more]
XP_022959177.15.8e-22184.04protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic [Cucurbita moschata] >XP_02... [more]
Match NameE-valueIdentityDescription
Q9M9038.1e-13350.94Protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Match NameE-valueIdentityDescription
A0A6J1E1S34.2e-22585.29protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Momordica charantia OX=3... [more]
A0A6J1KW751.7e-22184.26protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita maxima OX=3661... [more]
A0A6J1H3U02.8e-22184.04protein TRIGALACTOSYLDIACYLGLYCEROL 4, chloroplastic OS=Cucurbita moschata OX=36... [more]
A0A0A0K8242.8e-21379.96Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G001740 PE=4 SV=1[more]
A0A5D3BY401.5e-20979.53Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=... [more]
Match NameE-valueIdentityDescription
AT3G06960.15.8e-13450.94pigment defective 320 [more]
AT3G06960.25.0e-8551.31pigment defective 320 [more]
AT2G44640.11.7e-6432.26FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePANTHERPTHR34954:SF4PROTEIN TRIGALACTOSYLDIACYLGLYCEROL 4, CHLOROPLASTICcoord: 22..485
IPR044160Protein TRIGALACTOSYLDIACYLGLYCEROL 4-likePANTHERPTHR34954EXPRESSED PROTEINcoord: 22..485

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025541.1Sgr025541.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034196 acylglycerol transport
biological_process GO:1990052 ER to chloroplast lipid transport
biological_process GO:0001522 pseudouridine synthesis
biological_process GO:0008033 tRNA processing
cellular_component GO:0009941 chloroplast envelope
molecular_function GO:0070300 phosphatidic acid binding
molecular_function GO:0009982 pseudouridine synthase activity
molecular_function GO:0003723 RNA binding