Homology
BLAST of Sgr025536 vs. NCBI nr
Match:
XP_022138447.1 (kinesin-like protein KIN-12C isoform X3 [Momordica charantia])
HSP 1 Score: 3563.9 bits (9240), Expect = 0.0e+00
Identity = 1917/2329 (82.31%), Postives = 2049/2329 (87.98%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKL 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHKMKL
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMKL 600
Query: 601 MEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
MEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL
Sbjct: 601 MEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
Query: 661 LGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGE 720
LGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+LGE
Sbjct: 661 LGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYDLGE 720
Query: 721 REALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDA 780
REALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 REALLTEVAELRNELLVALGKNSTVSERDKYQ---------------------------- 780
Query: 781 KKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIG 840
DETMSIKSYSRDDT SYIT N E LENE G
Sbjct: 781 ------------------------------DETMSIKSYSRDDTLSYITENAENLENETG 840
Query: 841 QGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTK 900
QGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ NVTK
Sbjct: 841 QGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFNVTK 900
Query: 901 CQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQ 960
QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHVRQQ
Sbjct: 901 RQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHVRQQ 960
Query: 961 VEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMI 1020
VEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWETAMI
Sbjct: 961 VEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWETAMI 1020
Query: 1021 ELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLE 1080
ELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHKSLE
Sbjct: 1021 ELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHKSLE 1080
Query: 1081 DARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLN 1140
DARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDKR
Sbjct: 1081 DARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDKRFK 1140
Query: 1141 VKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDDVMA 1200
+KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD MA
Sbjct: 1141 FQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDDAMA 1200
Query: 1201 NTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDM 1260
TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LVQ+M
Sbjct: 1201 KTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELVQEM 1260
Query: 1261 VREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNI 1320
V+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNRLN+
Sbjct: 1261 VKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNRLNV 1320
Query: 1321 LEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSL 1380
LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY-------- 1380
Query: 1381 EGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGG 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++IGG
Sbjct: 1381 ---------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHDIGG 1440
Query: 1441 DSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIM 1500
D LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEAD+M
Sbjct: 1441 DPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEADLM 1500
Query: 1501 LNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFN 1560
LNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQVWF+
Sbjct: 1501 LNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQVWFS 1560
Query: 1561 LEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIG 1620
LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LEI G
Sbjct: 1561 LEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLEITG 1620
Query: 1621 REFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISK 1680
REFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L SK
Sbjct: 1621 REFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMILTSK 1680
Query: 1681 IDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTK 1740
ID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+KTK
Sbjct: 1681 IDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTAKTK 1740
Query: 1741 DMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKE 1800
DMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+KAKE
Sbjct: 1741 DMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEKAKE 1800
Query: 1801 TMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKLLLS 1860
TM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKLLLS
Sbjct: 1801 TMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKLLLS 1860
Query: 1861 VEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQK 1920
VEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYAEQK
Sbjct: 1861 VEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYAEQK 1920
Query: 1921 EEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLT 1980
EEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV NLT
Sbjct: 1921 EEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVGNLT 1980
Query: 1981 EVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVL 2040
E VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI ELVL
Sbjct: 1981 EAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILELVL 2040
Query: 2041 HADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCISNLV 2100
HAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCISNLV
Sbjct: 2041 HADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCISNLV 2100
Query: 2101 HQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
HQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL
Sbjct: 2101 HQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
Query: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAE 2220
TKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKKEAE
Sbjct: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKKEAE 2220
Query: 2221 ALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESRHAF 2280
AL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++ HAF
Sbjct: 2221 ALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNHHAF 2228
Query: 2281 LTK-QDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
LTK QDDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 LTKVQDDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2228
BLAST of Sgr025536 vs. NCBI nr
Match:
XP_022138448.1 (kinesin-like protein KIN-12C isoform X4 [Momordica charantia])
HSP 1 Score: 3561.9 bits (9235), Expect = 0.0e+00
Identity = 1916/2328 (82.30%), Postives = 2048/2328 (87.97%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKL 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHKMKL
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMKL 600
Query: 601 MEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
MEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL
Sbjct: 601 MEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
Query: 661 LGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGE 720
LGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+LGE
Sbjct: 661 LGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYDLGE 720
Query: 721 REALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDA 780
REALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 REALLTEVAELRNELLVALGKNSTVSERDKYQ---------------------------- 780
Query: 781 KKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIG 840
DETMSIKSYSRDDT SYIT N E LENE G
Sbjct: 781 ------------------------------DETMSIKSYSRDDTLSYITENAENLENETG 840
Query: 841 QGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTK 900
QGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ NVTK
Sbjct: 841 QGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFNVTK 900
Query: 901 CQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQ 960
QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHVRQQ
Sbjct: 901 RQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHVRQQ 960
Query: 961 VEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMI 1020
VEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWETAMI
Sbjct: 961 VEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWETAMI 1020
Query: 1021 ELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLE 1080
ELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHKSLE
Sbjct: 1021 ELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHKSLE 1080
Query: 1081 DARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLN 1140
DARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDKR
Sbjct: 1081 DARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDKRFK 1140
Query: 1141 VKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDDVMA 1200
+KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD MA
Sbjct: 1141 FQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDDAMA 1200
Query: 1201 NTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDM 1260
TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LVQ+M
Sbjct: 1201 KTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELVQEM 1260
Query: 1261 VREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNI 1320
V+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNRLN+
Sbjct: 1261 VKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNRLNV 1320
Query: 1321 LEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSL 1380
LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY-------- 1380
Query: 1381 EGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGG 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++IGG
Sbjct: 1381 ---------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHDIGG 1440
Query: 1441 DSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIM 1500
D LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEAD+M
Sbjct: 1441 DPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEADLM 1500
Query: 1501 LNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFN 1560
LNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQVWF+
Sbjct: 1501 LNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQVWFS 1560
Query: 1561 LEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIG 1620
LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LEI G
Sbjct: 1561 LEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLEITG 1620
Query: 1621 REFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISK 1680
REFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L SK
Sbjct: 1621 REFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMILTSK 1680
Query: 1681 IDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTK 1740
ID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+KTK
Sbjct: 1681 IDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTAKTK 1740
Query: 1741 DMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKE 1800
DMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+KAKE
Sbjct: 1741 DMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEKAKE 1800
Query: 1801 TMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKLLLS 1860
TM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKLLLS
Sbjct: 1801 TMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKLLLS 1860
Query: 1861 VEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQK 1920
VEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYAEQK
Sbjct: 1861 VEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYAEQK 1920
Query: 1921 EEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLT 1980
EEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV NLT
Sbjct: 1921 EEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVGNLT 1980
Query: 1981 EVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVL 2040
E VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI ELVL
Sbjct: 1981 EAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILELVL 2040
Query: 2041 HADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCISNLV 2100
HAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCISNLV
Sbjct: 2041 HADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCISNLV 2100
Query: 2101 HQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
HQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL
Sbjct: 2101 HQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
Query: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAE 2220
TKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKKEAE
Sbjct: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKKEAE 2220
Query: 2221 ALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESRHAF 2280
AL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++ HAF
Sbjct: 2221 ALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNHHAF 2226
Query: 2281 LTKQDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
LTK DDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 LTK-DDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2226
BLAST of Sgr025536 vs. NCBI nr
Match:
XP_022138445.1 (kinesin-like protein KIN-12C isoform X1 [Momordica charantia])
HSP 1 Score: 3558.5 bits (9226), Expect = 0.0e+00
Identity = 1917/2332 (82.20%), Postives = 2049/2332 (87.86%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHK--- 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHK
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMQQ 600
Query: 601 MKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
MKLMEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL
Sbjct: 601 MKLMEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
Query: 661 ELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYE 720
ELLLGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+
Sbjct: 661 ELLLGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYD 720
Query: 721 LGEREALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQ 780
LGEREALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 LGEREALLTEVAELRNELLVALGKNSTVSERDKYQ------------------------- 780
Query: 781 TDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLEN 840
DETMSIKSYSRDDT SYIT N E LEN
Sbjct: 781 ---------------------------------DETMSIKSYSRDDTLSYITENAENLEN 840
Query: 841 EIGQGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLN 900
E GQGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ N
Sbjct: 841 ETGQGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFN 900
Query: 901 VTKCQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHV 960
VTK QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHV
Sbjct: 901 VTKRQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHV 960
Query: 961 RQQVEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWET 1020
RQQVEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWET
Sbjct: 961 RQQVEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWET 1020
Query: 1021 AMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHK 1080
AMIELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHK
Sbjct: 1021 AMIELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHK 1080
Query: 1081 SLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDK 1140
SLEDARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDK
Sbjct: 1081 SLEDARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDK 1140
Query: 1141 RLNVKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDD 1200
R +KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD
Sbjct: 1141 RFKFQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDD 1200
Query: 1201 VMANTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLV 1260
MA TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LV
Sbjct: 1201 AMAKTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELV 1260
Query: 1261 QDMVREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNR 1320
Q+MV+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNR
Sbjct: 1261 QEMVKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNR 1320
Query: 1321 LNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHF 1380
LN+LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LNVLEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY----- 1380
Query: 1381 NSLEGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYN 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++
Sbjct: 1381 ------------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHD 1440
Query: 1441 IGGDSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEA 1500
IGGD LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEA
Sbjct: 1441 IGGDPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEA 1500
Query: 1501 DIMLNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQV 1560
D+MLNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQV
Sbjct: 1501 DLMLNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQV 1560
Query: 1561 WFNLEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLE 1620
WF+LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LE
Sbjct: 1561 WFSLEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLE 1620
Query: 1621 IIGREFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMML 1680
I GREFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L
Sbjct: 1621 ITGREFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMIL 1680
Query: 1681 ISKIDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTS 1740
SKID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+
Sbjct: 1681 TSKIDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTA 1740
Query: 1741 KTKDMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDK 1800
KTKDMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+K
Sbjct: 1741 KTKDMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEK 1800
Query: 1801 AKETMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKL 1860
AKETM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKL
Sbjct: 1801 AKETMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKL 1860
Query: 1861 LLSVEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYA 1920
LLSVEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYA
Sbjct: 1861 LLSVEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYA 1920
Query: 1921 EQKEEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVE 1980
EQKEEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV
Sbjct: 1921 EQKEEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVG 1980
Query: 1981 NLTEVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISE 2040
NLTE VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI E
Sbjct: 1981 NLTEAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILE 2040
Query: 2041 LVLHADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCIS 2100
LVLHAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCIS
Sbjct: 2041 LVLHADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCIS 2100
Query: 2101 NLVHQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
NLVHQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK
Sbjct: 2101 NLVHQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
Query: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKK 2220
LDLTKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKK
Sbjct: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKK 2220
Query: 2221 EAEALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESR 2280
EAEAL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++
Sbjct: 2221 EAEALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNH 2231
Query: 2281 HAFLTK-QDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
HAFLTK QDDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 HAFLTKVQDDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2231
BLAST of Sgr025536 vs. NCBI nr
Match:
XP_022138446.1 (kinesin-like protein KIN-12C isoform X2 [Momordica charantia])
HSP 1 Score: 3556.5 bits (9221), Expect = 0.0e+00
Identity = 1916/2331 (82.20%), Postives = 2048/2331 (87.86%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHK--- 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHK
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMQQ 600
Query: 601 MKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
MKLMEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL
Sbjct: 601 MKLMEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
Query: 661 ELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYE 720
ELLLGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+
Sbjct: 661 ELLLGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYD 720
Query: 721 LGEREALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQ 780
LGEREALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 LGEREALLTEVAELRNELLVALGKNSTVSERDKYQ------------------------- 780
Query: 781 TDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLEN 840
DETMSIKSYSRDDT SYIT N E LEN
Sbjct: 781 ---------------------------------DETMSIKSYSRDDTLSYITENAENLEN 840
Query: 841 EIGQGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLN 900
E GQGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ N
Sbjct: 841 ETGQGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFN 900
Query: 901 VTKCQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHV 960
VTK QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHV
Sbjct: 901 VTKRQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHV 960
Query: 961 RQQVEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWET 1020
RQQVEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWET
Sbjct: 961 RQQVEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWET 1020
Query: 1021 AMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHK 1080
AMIELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHK
Sbjct: 1021 AMIELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHK 1080
Query: 1081 SLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDK 1140
SLEDARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDK
Sbjct: 1081 SLEDARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDK 1140
Query: 1141 RLNVKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDD 1200
R +KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD
Sbjct: 1141 RFKFQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDD 1200
Query: 1201 VMANTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLV 1260
MA TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LV
Sbjct: 1201 AMAKTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELV 1260
Query: 1261 QDMVREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNR 1320
Q+MV+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNR
Sbjct: 1261 QEMVKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNR 1320
Query: 1321 LNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHF 1380
LN+LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LNVLEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY----- 1380
Query: 1381 NSLEGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYN 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++
Sbjct: 1381 ------------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHD 1440
Query: 1441 IGGDSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEA 1500
IGGD LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEA
Sbjct: 1441 IGGDPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEA 1500
Query: 1501 DIMLNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQV 1560
D+MLNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQV
Sbjct: 1501 DLMLNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQV 1560
Query: 1561 WFNLEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLE 1620
WF+LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LE
Sbjct: 1561 WFSLEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLE 1620
Query: 1621 IIGREFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMML 1680
I GREFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L
Sbjct: 1621 ITGREFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMIL 1680
Query: 1681 ISKIDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTS 1740
SKID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+
Sbjct: 1681 TSKIDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTA 1740
Query: 1741 KTKDMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDK 1800
KTKDMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+K
Sbjct: 1741 KTKDMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEK 1800
Query: 1801 AKETMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKL 1860
AKETM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKL
Sbjct: 1801 AKETMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKL 1860
Query: 1861 LLSVEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYA 1920
LLSVEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYA
Sbjct: 1861 LLSVEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYA 1920
Query: 1921 EQKEEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVE 1980
EQKEEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV
Sbjct: 1921 EQKEEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVG 1980
Query: 1981 NLTEVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISE 2040
NLTE VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI E
Sbjct: 1981 NLTEAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILE 2040
Query: 2041 LVLHADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCIS 2100
LVLHAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCIS
Sbjct: 2041 LVLHADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCIS 2100
Query: 2101 NLVHQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
NLVHQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK
Sbjct: 2101 NLVHQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
Query: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKK 2220
LDLTKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKK
Sbjct: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKK 2220
Query: 2221 EAEALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESR 2280
EAEAL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++
Sbjct: 2221 EAEALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNH 2229
Query: 2281 HAFLTKQDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
HAFLTK DDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 HAFLTK-DDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2229
BLAST of Sgr025536 vs. NCBI nr
Match:
QWT43323.1 (kinesin-like protein KIN12B [Citrullus lanatus subsp. vulgaris])
HSP 1 Score: 3494.5 bits (9060), Expect = 0.0e+00
Identity = 1879/2324 (80.85%), Postives = 2019/2324 (86.88%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSK +SAS S +QPESNEN G S S LHFPPPRTPFNIIADPAQ+HKE
Sbjct: 1 MSKHVSASKS----------SQPESNENELGISASGLHFPPPRTPFNIIADPAQFHKEFH 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGFDSNFKLQS KA WS DR+SEVSLK NGNAC+NNGTP+F AQGR +SEPSST STP
Sbjct: 61 DSGFDSNFKLQSTKAGWSPDRKSEVSLKINGNACSNNGTPRFLAQGRRASSEPSSTQSTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRISIPNTELPVEVPHFDLAEDPSF 180
A+SSSRVSFGGAIVATGSKAPQLADGRAGSS R RRISI NTELPV+VPHFDL EDPSF
Sbjct: 121 AKSSSRVSFGGAIVATGSKAPQLADGRAGSSSRFFRRISILNTELPVDVPHFDLEEDPSF 180
Query: 181 WNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQE 240
W DHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVW+GHPETRFTFDHIAC+TISQE
Sbjct: 181 WKDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWLGHPETRFTFDHIACETISQE 240
Query: 241 NLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEH 300
NLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEH
Sbjct: 241 NLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEH 300
Query: 301 LFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLT 360
LFTRIGMEEKSK+D KLKY CKCSFLEIYNEQI DLLEPSSTNLQLREDLKKGVYVENLT
Sbjct: 301 LFTRIGMEEKSKQDVKLKYSCKCSFLEIYNEQITDLLEPSSTNLQLREDLKKGVYVENLT 360
Query: 361 EHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARL 420
EH+VTT+NDVVKLLLQGAANRKMAAT+MNSESSRSHSVFTCIIESHWEKDSRTHFRFARL
Sbjct: 361 EHSVTTINDVVKLLLQGAANRKMAATYMNSESSRSHSVFTCIIESHWEKDSRTHFRFARL 420
Query: 421 NLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLT 480
NLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSRLT
Sbjct: 421 NLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSRLT 480
Query: 481 FLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQ 540
FLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETALQ
Sbjct: 481 FLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETALQ 540
Query: 541 RQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKLME 600
RQILHLKGQLSFLLKHSNFPRSI+++V R EESGVS LF+ YD LGGRMQT N KMKLME
Sbjct: 541 RQILHLKGQLSFLLKHSNFPRSILNSVPRLEESGVSALFEGYDALGGRMQTENRKMKLME 600
Query: 601 ATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLG 660
A+LIGALRREEVAN I+KLEFEIEHMK LAFQQEED QRTKMLLKFREEKIRQLEL LG
Sbjct: 601 ASLIGALRREEVANATIKKLEFEIEHMKCLAFQQEEDGQRTKMLLKFREEKIRQLELFLG 660
Query: 661 GMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGERE 720
GMLSAD+YLLEENKAL VEIKMLQAKINR+PELTRVS ENS+L EQLQV+QNFYELGERE
Sbjct: 661 GMLSADQYLLEENKALAVEIKMLQAKINRNPELTRVSFENSKLTEQLQVYQNFYELGERE 720
Query: 721 ALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDAKK 780
LLTEV+ELRNE
Sbjct: 721 VLLTEVSELRNE------------------------------------------------ 780
Query: 781 YLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIGQG 840
DETMSIKSY++DDT SYITG+DE EN IGQG
Sbjct: 781 ----------------------------DETMSIKSYTQDDTLSYITGSDENFENTIGQG 840
Query: 841 SDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTKCQ 900
SDN+ KPICSQ+DLID MLA+++DS+N MQ E+H CKQ KH M ENF+ K NVTKCQ
Sbjct: 841 SDNELGAKPICSQKDLIDAKMLAQSMDSDNTMQAENHGCKQFKHCMVENFLNKSNVTKCQ 900
Query: 901 NKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQVE 960
N+GNV++QHEDVDNK LQVKLENLT+ELEEVRLSNIQYQENQ +QQNQ E VRQQVE
Sbjct: 901 NEGNVMNQHEDVDNKTLQVKLENLTRELEEVRLSNIQYQENQ----NQQNQSEDVRQQVE 960
Query: 961 METANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMIEL 1020
METANTILQLQEEVETLQLELNDRLHGLAQENTRLKD LSAK+EEMRMLC+DWETAM+EL
Sbjct: 961 METANTILQLQEEVETLQLELNDRLHGLAQENTRLKDLLSAKHEEMRMLCIDWETAMVEL 1020
Query: 1021 TSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLEDA 1080
T+FLLD S+SIRDAHGQIE I+NLFPKVNVGISEQVQ+AI+VC+EKEETILLLHKSLEDA
Sbjct: 1021 TNFLLDGSKSIRDAHGQIESIANLFPKVNVGISEQVQQAIKVCIEKEETILLLHKSLEDA 1080
Query: 1081 RLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLNVK 1140
RLMV+EMELKLDSLKEATLALNESQQM DN AA AK LS +TD+NIMVEFL KRL VK
Sbjct: 1081 RLMVKEMELKLDSLKEATLALNESQQMDDNISAAGAKPLSTQMTDENIMVEFLGKRLGVK 1140
Query: 1141 KDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDDVMANT 1200
DQL EA S +W S QELACC +I REMPISKLDVS Q SSHI DDVMAN
Sbjct: 1141 NDQLSEAEKSADAAVIAVEWSSQPQELACCYNIEREMPISKLDVSSQRSSHIFDDVMANV 1200
Query: 1201 NALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDMVR 1260
N LLLEE DTES++I LG+ ELKNITS HYADMEMHISAL+IYI +LYSEY LVQDMV
Sbjct: 1201 NELLLEESDTESNMIWLGLAELKNITSGHYADMEMHISALHIYIQNLYSEYQDLVQDMVS 1260
Query: 1261 EIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNILE 1320
EIH LRLKAKT+NENCK LQFFKDK AHKYWN+ENQNS+LDQIKAKIYEAKNRLNILE
Sbjct: 1261 EIHVLRLKAKTSNENCKSLQFFKDKDQPAHKYWNVENQNSILDQIKAKIYEAKNRLNILE 1320
Query: 1321 DSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSLEG 1380
DSIDRNI GCG QY+ QYPV EDGWSSDCSTSSSDISTESVTSRG
Sbjct: 1321 DSIDRNIVGCGEQYLDQYPVKEDGWSSDCSTSSSDISTESVTSRG--------------- 1380
Query: 1381 DSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGGDS 1440
KLLD MNGD GTTTCLR+EL MTYN IHKL +QIDA M++ GGDS
Sbjct: 1381 --------------KLLDYMNGDKGTTTCLRKELYMTYNVIHKLCLQIDALLMHDTGGDS 1440
Query: 1441 LSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIMLN 1500
L+EEMDQ KT+ KLRM AEAGCNN S+V S++E K+DGGFL R EEAQ AI+EADIMLN
Sbjct: 1441 LAEEMDQGKTSFKLRMEKAEAGCNNTSQVTSVEETKEDGGFLDRLEEAQEAIREADIMLN 1500
Query: 1501 ALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFNLE 1560
ALLRANENAKQ TGIFKQAGEQLQIER+NLI+EVGQLKSSMHLKDA+NKLL EQV FNLE
Sbjct: 1501 ALLRANENAKQQTGIFKQAGEQLQIERENLIDEVGQLKSSMHLKDAENKLLHEQVCFNLE 1560
Query: 1561 EVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIGRE 1620
EVA+ VSSLEGCISQTQR+VDEKFGI+SCD+ISFR+EMLKS+ NWKSL+ED+ LE++GRE
Sbjct: 1561 EVASSVSSLEGCISQTQRDVDEKFGIISCDIISFRDEMLKSVGNWKSLLEDVFLEVMGRE 1620
Query: 1621 FASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISKID 1680
F SFV+H+C V E+C QFA+FKAEP+F LK QRC+ES N S STC+ DK+D+MLISKID
Sbjct: 1621 FMSFVVHQC-VNEICWQFAQFKAEPNFLPLKWQRCLESTNISGSTCLTDKEDIMLISKID 1680
Query: 1681 RGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTKDM 1740
RGR+EL+TGLEEAD GFSYDD+LYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTKDM
Sbjct: 1681 RGRTELITGLEEADGGFSYDDMLYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTKDM 1740
Query: 1741 KDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETM 1800
K++ DYSLSQL+HELEIKA+QL++VLVQQRKLEGLLTDTEKALF SNSKLDKAKETM
Sbjct: 1741 KNER---DYSLSQLQHELEIKANQLDSVLVQQRKLEGLLTDTEKALFSSNSKLDKAKETM 1800
Query: 1801 TIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSVEKKLLLSVEDI 1860
T ISEHNAQLKKQVEDL+LKKSEA KQLA+QQDV+N+LENEI+H T S+EKK +LSVEDI
Sbjct: 1801 TSISEHNAQLKKQVEDLYLKKSEAGKQLAEQQDVINKLENEIIHLT-SLEKKSVLSVEDI 1860
Query: 1861 ENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQKEEEV 1920
ENEL RVISERDQL EQVCFLTDKL IAYALADEKEAVA EARQESEASKLYAEQKEEEV
Sbjct: 1861 ENELSRVISERDQLHEQVCFLTDKLGIAYALADEKEAVATEARQESEASKLYAEQKEEEV 1920
Query: 1921 KILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLTEVVD 1980
KILEHSVEELESTINMLETKVHEMDEEVEKN TLRESLELE QIFR+RLLAV++L+E VD
Sbjct: 1921 KILEHSVEELESTINMLETKVHEMDEEVEKNRTLRESLELEKQIFRQRLLAVQSLSEDVD 1980
Query: 1981 SGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVLHADV 2040
GDEIVEHAQEQPRQ G I+LELL+ RSRIKLLEEER EQDKE+KR KEYISELVLHAD
Sbjct: 1981 CGDEIVEHAQEQPRQAGIIVLELLETRSRIKLLEEERVEQDKEVKRLKEYISELVLHADA 2040
Query: 2041 QAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCISNLVHQMN 2100
QAMKYQQKYTNLE MVRDASKD SNP+TA T+DK++K+S RPRGSSSPFRCISNLVHQMN
Sbjct: 2041 QAMKYQQKYTNLEVMVRDASKDHSNPMTAPTLDKVEKNSARPRGSSSPFRCISNLVHQMN 2100
Query: 2101 LEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYA 2160
LEK+HELSTARLRIEELEGL+TSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYA
Sbjct: 2101 LEKEHELSTARLRIEELEGLSTSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYA 2160
Query: 2161 NLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAEALST 2220
N I+QYQVQ+MV+EAHLQSQQFREKEQE+H LRTQI+DL EERECYKSVLSKKEAEAL+T
Sbjct: 2161 NFIEQYQVQQMVMEAHLQSQQFREKEQEVHNLRTQINDLQEERECYKSVLSKKEAEALAT 2199
Query: 2221 QIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESRHAFLTKQ 2280
QIACEKLRERDHLLSVQNGILKMENKNLKR+IVELD KANTLHQTRIS QE+RH FLTK
Sbjct: 2221 QIACEKLRERDHLLSVQNGILKMENKNLKRKIVELDGKANTLHQTRISQQENRHTFLTK- 2199
Query: 2281 DDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
+DELTKRLA+SKM+LSRVNDEIARY IPSGSSSHHRSGGSGNEI
Sbjct: 2281 NDELTKRLANSKMLLSRVNDEIARYRIPSGSSSHHRSGGSGNEI 2199
BLAST of Sgr025536 vs. ExPASy Swiss-Prot
Match:
Q27IK7 (Kinesin-like protein KIN-12C OS=Arabidopsis thaliana OX=3702 GN=KIN12C PE=1 SV=1)
HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 999/2312 (43.21%), Postives = 1365/2312 (59.04%), Query Frame = 0
Query: 13 LDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQDSGFDSNFKLQS 72
+ R+ PES EN F S F P R P N I DP+Q K FD KL+
Sbjct: 1 MSRNVPRIEMPESEENEFA---SLSLFSPSRPPLNSIPDPSQIQKANHLPHFDLVQKLEG 60
Query: 73 IKAD----WSSDRRSEVSLKNGNACTNNGTPKFSAQGRWVNSEPSSTHSTPARSSSRVSF 132
+A +++ EV L+ +++ PK + SEP+S STP R+ +RVS
Sbjct: 61 TRAQHQRTLGPEKKFEV-LEGRAGNSSDSNPKIVNRNGKSRSEPNSAQSTPTRNGARVSL 120
Query: 133 GGAIVATGSKAPQLADGRAGSSFRLSRRISIPNTELPVE-VPHFDLAEDPSFWNDHNVQV 192
GG ATG++ Q GR R+ R +SI + E PHF+L ED SFW DHNVQV
Sbjct: 121 GGG-CATGARFLQSFGGRG----RIPRGVSIAESVSFAETTPHFELNEDHSFWKDHNVQV 180
Query: 193 MIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQENLFKVAGQ 252
+IR+RPL T ER +QGYG+CL+QE+ +TLVW+GHPE RFTFDH+A +TISQE LF+VAG
Sbjct: 181 LIRLRPLGTMERANQGYGKCLKQESPQTLVWLGHPEARFTFDHVASETISQEKLFRVAGL 240
Query: 253 PMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEHLFTRIGME 312
PMVENCLSGYNSC+FAYGQTGSGKTYTMMG I E EG L EDCG+T RIFE+LF+RI ME
Sbjct: 241 PMVENCLSGYNSCVFAYGQTGSGKTYTMMGEISEAEGSLGEDCGVTARIFEYLFSRIKME 300
Query: 313 EKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLTEHNVTTVN 372
E+ +RD LK+ CKCSFLEIYNEQI DLLEPSSTNLQLREDL KGVYVENL EHNV TV+
Sbjct: 301 EEERRDENLKFSCKCSFLEIYNEQITDLLEPSSTNLQLREDLGKGVYVENLVEHNVRTVS 360
Query: 373 DVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARLNLVDLAGS 432
DV+KLLLQGA NRK+AAT MNSESSRSHSVFTC IES WEKDS T RFARLNLVDLAGS
Sbjct: 361 DVLKLLLQGATNRKIAATRMNSESSRSHSVFTCTIESLWEKDSLTRSRFARLNLVDLAGS 420
Query: 433 ERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLG 492
ERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRH+PYRDSRLTFLLQDSLG
Sbjct: 421 ERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHVPYRDSRLTFLLQDSLG 480
Query: 493 GNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQRQILHLKG 552
GNSKT +IANVSPS CS NETLSTLKFAQRAK IQNNAKVNE AS D TALQ++I LK
Sbjct: 481 GNSKTMIIANVSPSLCSTNETLSTLKFAQRAKLIQNNAKVNEDASGDVTALQQEIRKLKV 540
Query: 553 QLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKLMEATLIGALR 612
QL+ LLK+ + ++ + EES S K R + ++K M +IGALR
Sbjct: 541 QLTSLLKNHDSCGALSDCISSLEESRYSGTCKVAGET--RQDKCHCQVKNMNDNMIGALR 600
Query: 613 REEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLGGMLSADKY 672
RE++A +A+QK E EIE + L EEDA+R K++L REEK+ ++E G L +
Sbjct: 601 REKIAESALQKSEAEIERIDCLVRDMEEDAKRIKIMLNLREEKVGEMEFCTSGSLMTKEC 660
Query: 673 LLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGEREALLTEVAE 732
L+EENK L EIK+L+ I+++PELTR +LEN++L EQLQ +Q FYE GEREALL EV
Sbjct: 661 LIEENKTLKGEIKLLRDSIDKNPELTRSALENTKLREQLQRYQKFYEHGEREALLAEVTG 720
Query: 733 LRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDAKKYLKCSQGY 792
LR++LL + +D+ H K NE++ K++ C
Sbjct: 721 LRDQLL------DVLEAKDESFSKHVMK--------------ENEME---KEFEDCRNMN 780
Query: 793 ASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIGQGSDNDFSVK 852
+S+ Q G ++ D+ QS + + R G++
Sbjct: 781 SSLIRELDEIQAGLGRYLNFDQ-----------IQSNVVASSTR-------GAEQ----- 840
Query: 853 PICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTKCQNKGNVVDQ 912
AET+ + + +Q E ++ +K ++G +V
Sbjct: 841 --------------AETMPTISEIQEE----------------VAISHSKNYDRGALVKT 900
Query: 913 HEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQVEMETANTIL 972
E +D LQ KL L K+LEE R N +Y+++ QLSQQ IE VR+QVE ETA TIL
Sbjct: 901 DEGIDRSILQFKLGKLMKDLEEARTLNCKYEKDHKSQLSQQEDIEVVREQVETETARTIL 960
Query: 973 QLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMIELTSFLLDSS 1032
+LQEEV LQ E R+ L +EN +KD ++A+ E+R L DWE A +ELT+F++ S
Sbjct: 961 ELQEEVIALQSEFQRRICNLTEENQSIKDTITARESEIRALNQDWEKATLELTNFIVAGS 1020
Query: 1033 RSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLEDARLMVQEME 1092
+SI++A QIE I FP+VN I + V++A + C++KEETILLL KSLEDAR++V EM
Sbjct: 1021 KSIKNASTQIESIICSFPQVNAWIGDYVEKAAKNCIKKEETILLLQKSLEDARILVAEMN 1080
Query: 1093 LKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLNVKKDQLVEAS 1152
LKL+SLK AT+ALNE Q AA ++ +L D + M + +D
Sbjct: 1081 LKLNSLKGATIALNEFQL---GGNAATTEEAFNLNNDVDRMSDEVD-------------- 1140
Query: 1153 LSQWLSYSQELACCNSIGREMPISKLDVSFQSSHIIDDVMANTNALLLEELDTESDLICL 1212
L+ +F+ AN ++L E E+ L
Sbjct: 1141 ------------------------TLESNFK---------ANQYSILKTERHAEAAL--- 1200
Query: 1213 GIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDMVREIHELRLKAKTTNENCK 1272
A+ ++ D ++ M+ ++ + +K T
Sbjct: 1201 ---------------------AVTKWLSDSRDQH-----QMMEKVQDQSVKEFGT----- 1260
Query: 1273 RLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNILEDSIDRNIAGCGYQYIGQ 1332
L I A + E + D +++ G+
Sbjct: 1261 ------------------------LSSISASL--------SAEGNADISLSRDGHLSDAT 1320
Query: 1333 YPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSLEGDSVICELCKKVESVKLL 1392
YP + + STSSSD S LN + C +S E D+ E K+ S L+
Sbjct: 1321 YPKGD-----ELSTSSSDFSNCRWQHDCALN-VKCQGVSSSESDAQ--ESNNKITSAALI 1380
Query: 1393 DSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGGDSLSEEMDQAKTTPKLRMG 1452
+ NG + + C + S++ M + E + K + L G
Sbjct: 1381 -AKNGSAHSVYC----------GEGRQSVEKPLTIM-------MGREETEYKCSKPLSSG 1440
Query: 1453 NAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIMLNALLRANENAKQLTGIFK 1512
+ V + F RFEE A +KEAD+ + L++ANE + +T ++
Sbjct: 1441 VYMGLMQRMDPVRT---------FFDRFEEVNATMKEADLTICELVKANEKSNSVTEMWL 1500
Query: 1513 QAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFNLEEVANLVSSLEGCISQTQ 1572
Q E+L + NL++++ Q+KS + + + ++L Q L ++ N VS LE + +
Sbjct: 1501 QTHEELISKEKNLMDDLEQVKSILSACEEEKQVLLNQTHTTLADMENSVSLLEEYFQEMK 1560
Query: 1573 REVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIGREFASFVLHECYVKEVCCQ 1632
R V+E + E+L+ ISN + +E I E + REF + ++C++ ++ Q
Sbjct: 1561 RGVEETVEALFSHARLAGKELLQLISNSRPSLEQIASEFMEREFTMYATYQCHIGKLIDQ 1620
Query: 1633 FARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISKIDRGRSELVTGLEEADAGF 1692
+ + L Q E+N + + I + + K + R E+VTGLE +
Sbjct: 1621 ILDQRKQVITPNLSGQ---ETNQSVKINAIGYNAEDEVTKK--QSREEIVTGLENDEVVQ 1680
Query: 1693 SYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTKDMKDQTEKLDYSLSQLEHE 1752
S++ +LYENL LKKEL+RKE L EGLLFDFRLLQES S +D+K++ ++L +L +++ E
Sbjct: 1681 SHESLLYENLYLKKELERKEALFEGLLFDFRLLQESASNKRDIKNEMDELFDALCKVQLE 1740
Query: 1753 LEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMTIISEHNAQLKKQVEDL 1812
LE+KASQ+ + V LE D + ALF S S L++AK+ + I++E N +L+ V DL
Sbjct: 1741 LELKASQVHELFVHNENLENCSIDLKTALFTSQSDLEQAKQRIQILAEQNDELRALVSDL 1800
Query: 1813 HLKKSEAKKQLADQQDVVNRLENEILHRTSSVEKKLLLSVEDIENELRRVISERDQLREQ 1872
+K+ A++ L +Q+D+VNRLE EILH T++ EK+LL +V+ I+ L++ E+DQ+ ++
Sbjct: 1801 CKEKAAAEEGLDEQRDLVNRLEKEILHLTTTAEKQLLSAVKSIKENLKKTSDEKDQIVDE 1860
Query: 1873 VCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQKEEEVKILEHSVEELESTINML 1932
+C L +KL++AYA+ADEKEA+A+EA QESEASK+YAEQKEEEVKILE SVEELE TIN+L
Sbjct: 1861 ICSLNNKLELAYAIADEKEAIAVEAHQESEASKIYAEQKEEEVKILEISVEELERTINIL 1920
Query: 1933 ETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLT-EVVDSGDEIVEHAQEQPRQQ 1992
E +V++MDEEV+++ T ++SLE E Q R+RL EN T +V + + E+ R
Sbjct: 1921 ERRVYDMDEEVKRHRTTQDSLETELQALRQRLFRFENFTGTMVTTNESTEEYKSHISRST 1980
Query: 1993 GSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVLHADVQAMKYQQKYTNLEAMV 2052
G L A S+I++L++E AEQ KEIK+ KEYISE++LH++ Q+ YQ+KY LE M+
Sbjct: 1981 G-----LQGAHSQIQVLQKEVAEQTKEIKQLKEYISEILLHSEAQSSAYQEKYKTLEVMI 2040
Query: 2053 RDASKDQSNPITATTID-KIDKSSVRPRGSSSPFRCISNLVHQMNLEKDHELSTARLRIE 2112
RD + S+ A TI K +KSS R RGSSSPFRCI LV QM LEKD EL+ AR+R+E
Sbjct: 2041 RDFKLEDSSSSAAETISHKTEKSSTRSRGSSSPFRCIVGLVQQMKLEKDQELTMARVRVE 2061
Query: 2113 ELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYANLIDQYQVQKMVVEA 2172
ELE L +QKEIC LN R+AAA+SMTHDVIRDLLGVK+D+T YA LIDQ+QVQ++V +A
Sbjct: 2101 ELESLLAVKQKEICTLNTRIAAADSMTHDVIRDLLGVKMDITSYAELIDQHQVQRVVEKA 2061
Query: 2173 HLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAEALSTQIACEKLRERDHLLS 2232
+++ KEQE+ L+ I L ++RE S L+KK+ + L+TQI+ ++L+ER LLS
Sbjct: 2161 QQHAEEILSKEQEVMNLKRHIDYLFKDRESCMSELNKKDTDVLATQISLDQLQERVQLLS 2061
Query: 2233 VQNGILKMENKNLKRRIVELDEKANTLHQTRIS----PQESRH-AFLTKQDDELTKRLAH 2292
+QN +LK + NL R++ ELD T+H + S PQ ++ A D + TKRL +
Sbjct: 2221 MQNEMLKNDKSNLLRKLAELD---RTVHNAQASNHRVPQTTKDTASFKLADTDYTKRLEN 2061
Query: 2293 SKMVLSRVNDEIARYHIPSGSSSHHRSGGSGN 2313
++ +LS N+E+A+Y S + R+ G +
Sbjct: 2281 AQKLLSHANNELAKYRKTSNNHPSTRTQGQSS 2061
BLAST of Sgr025536 vs. ExPASy Swiss-Prot
Match:
B9FUF9 (Kinesin-like protein KIN-12E OS=Oryza sativa subsp. japonica OX=39947 GN=KIN12E PE=2 SV=2)
HSP 1 Score: 1094.0 bits (2828), Expect = 0.0e+00
Identity = 799/2214 (36.09%), Postives = 1189/2214 (53.70%), Query Frame = 0
Query: 129 GGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDPSFWNDHNVQ 188
GG +T A + + + L+ + + E+P EVPHF+L EDP+FW D NVQ
Sbjct: 7 GGRRASTSRAAARRVEAETNENDDLAAAAARDLAAAEVPAEVPHFELDEDPAFWKDRNVQ 66
Query: 189 VMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQENLFKVAG 248
V+IRIRP++ E + G RCL Q+++KTL W GHP+T FTFDH+AC+TISQE LF V G
Sbjct: 67 VLIRIRPINAAESTANGQRRCLVQDSSKTLSWTGHPDTMFTFDHVACETISQEKLFGVVG 126
Query: 249 QPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEHLFTRIGM 308
PMVENC+SGYN C+FAYGQTGSGKTYTMMG + +++ +L++D GLT RIFE+LF RI
Sbjct: 127 LPMVENCMSGYNGCLFAYGQTGSGKTYTMMGELSKLDNELSKDSGLTPRIFEYLFARIKE 186
Query: 309 EEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLTEHNVTTV 368
EE+ +R+ KLKY CKCSFLEIYNEQI DLLEPSSTNLQ+RED+KKGVYVENL E V++V
Sbjct: 187 EEERRREDKLKYICKCSFLEIYNEQITDLLEPSSTNLQIREDIKKGVYVENLMECYVSSV 246
Query: 369 NDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARLNLVDLAG 428
DV+ LLLQG ANRKMAAT+MNSESSRSHSVFTC+IES WE+DS TH RF RLNLVDLAG
Sbjct: 247 KDVMMLLLQGVANRKMAATNMNSESSRSHSVFTCVIESRWERDSMTHLRFGRLNLVDLAG 306
Query: 429 SERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSL 488
SERQKSSGAEG+RLKEAANIN+SLST+GLVIM+LVD+A+GK+RH+PYRDSRLTFLLQDSL
Sbjct: 307 SERQKSSGAEGERLKEAANINRSLSTLGLVIMTLVDVANGKNRHVPYRDSRLTFLLQDSL 366
Query: 489 GGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQRQILHLK 548
GGNSKTT++ANVSPS CS++ETLSTLKFAQRAK IQNNAKVNE AS D +LQRQI LK
Sbjct: 367 GGNSKTTIVANVSPSICSSSETLSTLKFAQRAKLIQNNAKVNEDASGDVMSLQRQIEDLK 426
Query: 549 GQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDY----DPLGGRMQTANHKMKLMEATL 608
QL+ L K N P S + + SG + F D ++ K+ +E L
Sbjct: 427 DQLTCLKKQQNMPGSPSFKLLK---SGYGNEFNSLHGVDDQSACDLELLKQKVIHLEDVL 486
Query: 609 IGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLGGML 668
+G+LRRE+ A T I+KLE EI+ + RL E D + + +K R+EKIR+LELL +
Sbjct: 487 VGSLRREKSAETEIRKLECEIKSLNRLVNLMESDTRHLRTTVKLRDEKIRRLELLADNQI 546
Query: 669 SADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGEREALL 728
S+D YL++EN A+ EI++LQ +IN + +LT+ +LEN LIEQ+++ + F + GERE LL
Sbjct: 547 SSDGYLMDENAAMFQEIQLLQEQINDNSQLTQFALENKRLIEQVRMLEKFSKQGEREMLL 606
Query: 729 TEVAELRNELLVAL-------GKNSTISERDKYQDFHTTKGLIN--LENPDTRQIGGNEL 788
TE++ LRN L L KN ++ T + ++ LEN N+L
Sbjct: 607 TEISLLRNHFLHILEQKYARPPKNMEAQGDVTIKELETCRKELDACLENNVLLAREVNKL 666
Query: 789 QTDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDT---QSYITGND- 848
+ + K+Y KC G + + + I D SY+ D
Sbjct: 667 RCELKQYQKCGTGQVAPEV------------VESSVIPGINQKQHDQAGWCGSYLASIDV 726
Query: 849 ERLENEIGQGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENF 908
ER ++G +D S++ E + +E DS + + D +K+ +
Sbjct: 727 ERQFVDVGITTDITESLELTPPSE------IYSENQDSPSRLHFSDPEICDLKNSTK--- 786
Query: 909 IKKLNVTKCQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQN 968
V + + N++D+ L +LEN E L+++Q E + + + +
Sbjct: 787 -----VLEYNSSRNLLDK-----GIILSGQLEN------ECGLNSVQNDEISLVKENAEK 846
Query: 969 QIEHVRQQVEMETANTILQ-----LQEE---VETLQLELNDRLHGLAQENTRLKDALSAK 1028
H ++ + N IL LQ+E +++L L ++L +A+E+T+L + + AK
Sbjct: 847 MYGH--DEISVYRQNEILHSSEQLLQDELTHIKSLNEGLKEKLIIMAEESTKLSEIIVAK 906
Query: 1029 NEEMRMLCVDWETAMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEV 1088
+ E+ L +WE+A+++LTSFL D S+ DA+ I+ + + FP N +SE V++A++V
Sbjct: 907 DVEIATLSEEWESAIVDLTSFLTDGCSSLDDAYQNIDNMISSFPYNNHSVSEHVEKAMKV 966
Query: 1089 CVEKEETILLLHKSLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHL 1148
+EKE+ I L L+ A+ M +E++ KL L+ ATLA+ E+ Q+ DN + +A +L L
Sbjct: 967 SIEKEKIISRLQIELQAAQRMGREVKEKLHILRGATLAITEA-QLLDNDESQEALKLLDL 1026
Query: 1149 LTDKNIMVEFLDKRLNVKKDQLVEASLSQWLSYSQELACCNSIGREMPISKLDVSFQSSH 1208
+ K+ V+ L+ NVK+
Sbjct: 1027 MRQKDCTVQELND--NVKQK---------------------------------------- 1086
Query: 1209 IIDDVMANTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEY 1268
S L+ + YS +
Sbjct: 1087 ----------------------------------------------SCLFAEATEGYSRH 1146
Query: 1269 HGLVQDMVREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYE 1328
+ D V + E+ +H
Sbjct: 1147 ECHLPDNVGTVAEI-----------------------SH--------------------- 1206
Query: 1329 AKNRLNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLL 1388
N DG S+++ + + KL +L
Sbjct: 1207 -----------------------------NRDG---------SEVNQANTHYQAKLEDVL 1266
Query: 1389 CSHFNSLEGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAF 1448
Sbjct: 1267 ------------------------------------------------------------ 1326
Query: 1449 QMYNIGGDSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAA 1508
L E+ +KV+++ + FEEAQ
Sbjct: 1327 --------HLVEDKS--------------------NKVLAL---------FSNFEEAQET 1386
Query: 1509 IKEADIMLNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLL 1568
++EA+ ML++LL+ANE K +QA E L ER +LI ++ +L++S L
Sbjct: 1387 MEEAETMLSSLLKANEELKLEKDSCRQAVELLFAERTSLINDLQELEASNSFTAQRYDKL 1446
Query: 1569 QEQVWFNLEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMED 1628
EQV + E+ NL + ++ Q QR + +VISF ++ K I +S + +
Sbjct: 1447 HEQVNGCVAEMTNLATIIKESFHQVQRVTTVELFAFCSEVISFGQDLRKWIYESRSYLVN 1506
Query: 1629 I--CLEIIGREFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIAD 1688
+ LE G +A E + +A + +Q M + + + D
Sbjct: 1507 MGALLEEQGNSYA-----EQNRRTNSSTYAGVSQQVE-SCSRQLGGMNGDIFPGTYMVVD 1566
Query: 1689 KDDMMLISKIDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRL 1748
+ + + G + LE+ + ++D + +L++E RK + EGL FD +L
Sbjct: 1567 GKEKASVHVVPFGSN---AELEDTNVERTFD---MDYASLRREFDRKSDVAEGLSFDLKL 1626
Query: 1749 LQESTSKTKDMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLS 1808
LQESTS+ KDMKD+ +++ +L ++ ELE K S +E++L QQ+ LE L + AL +
Sbjct: 1627 LQESTSQAKDMKDKADEISDALVSVQRELEKKTSAMESILKQQKVLEEELAENGAALLIL 1686
Query: 1809 NSKLDKAKETMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV 1868
S+L+ ++ + + + N LK +E+ + SE K L D+ V+ LE+EIL SS
Sbjct: 1687 RSELEHSESLSSELFKENNNLKVMLEEEAMMISETKAMLEDKSKVIEGLEHEILLLNSSE 1746
Query: 1869 EKKLLLSVEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEAS 1928
E +L+ ++++ + L+ + ++ L E++ LTDKL++A ALA+E EA +IEARQ +E S
Sbjct: 1747 EGRLMSQIKELNDNLKIISIDKGNLEEEILKLTDKLEMAVALAEENEAASIEARQAAEIS 1806
Query: 1929 KLYAEQKEEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRL 1988
K+YAE+KEEEV+ILE SVEELESTI +LE +V + EEV ++S + Q
Sbjct: 1807 KVYAEEKEEEVRILERSVEELESTITVLEEEVCNLKEEVRSYQIYKKSEAEQAQ-----E 1866
Query: 1989 LAVENLTEVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKE 2048
+ + + T D+ +++ + ++ + I+ DAR +I+ L E + +D+E++++KE
Sbjct: 1867 MFIVDSTSKCDATEQLCPGRCQLEKRLKAEIIAHQDARRKIECLTMEASCKDEEVRQYKE 1889
Query: 2049 YISELVLHADVQAMKYQQKYTNLEAMV--RDASKDQSNPITATTIDKIDKSSVRPRGSSS 2108
+I+ELVLH++ Q++ +Q+KY +E M+ + +SN T T K +K S R RGS S
Sbjct: 1927 HIAELVLHSEAQSLLFQEKYQEMEHMISKQKFGLHESNSDTGHT--KFEKPSGRTRGSGS 1889
Query: 2109 PFRCISNLVHQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIR 2168
PFRCIS++V QMN EKD E+S AR RIEELEGL ++QKEIC+L +RLAA +SMTHD+IR
Sbjct: 1987 PFRCISSIVQQMNSEKDQEISVARQRIEELEGLVCNKQKEICMLTSRLAAVDSMTHDIIR 1889
Query: 2169 DLLGVKLDLTKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYK 2228
+LLGVKLD+T YAN++DQ ++QK+++ + Q +Q + K+ E+ L+ Q L +ER+
Sbjct: 2047 ELLGVKLDMTNYANMLDQEELQKLLMASQQQIEQSKAKDVELDMLKEQFDHLIQERDSLF 1889
Query: 2229 SVLSKKEAEALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRI 2288
+ +++A+ L +Q+ E+L +R+ +L QNGIL+ME NL++RI+E+DE+ L +
Sbjct: 2107 DDMDQRKADLLESQLLIEQLEQREQMLEAQNGILQMEKDNLQQRIMEMDEEIQLLVGSNQ 1889
Query: 2289 SPQESRHAFLTKQ---DDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSG 2309
+ E+ + + E ++RLA S M+LS E +R + + SS R G
Sbjct: 2167 AIAETTFQMGSNHRSANSEFSRRLAQSDMLLSHARHEHSR--LQAAKSSRTRRG 1889
BLAST of Sgr025536 vs. ExPASy Swiss-Prot
Match:
B9GE13 (Kinesin-like protein KIN-12F OS=Oryza sativa subsp. japonica OX=39947 GN=KIN12F PE=3 SV=1)
HSP 1 Score: 1043.9 bits (2698), Expect = 2.7e-303
Identity = 861/2581 (33.36%), Postives = 1303/2581 (50.48%), Query Frame = 0
Query: 114 STHSTPARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRISIPNTEL---PV---- 173
ST STP +S ++ ++ + + P ++ G+ G+ L ++ T + PV
Sbjct: 134 STQSTPTKSVTKPAYS---IGMSASRPPMSSGQRGAGLGLGFSMAARGTPMSFAPVTVVN 193
Query: 174 --EVPHFDLAEDPSFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPET 233
EVPHF+L EDPSFW ++NVQV+IR+RPL+ TER+ Y RCL+QE+A+++ WIG PE+
Sbjct: 194 TAEVPHFELREDPSFWMENNVQVVIRVRPLNNTERNLHNYNRCLKQESAQSITWIGQPES 253
Query: 234 RFTFDHIACDTISQENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG 293
RFTFDH+AC+ ++QE LF+VAG PMVENC++GYNSC+FAYGQTGSGKTYTM+G I E+E
Sbjct: 254 RFTFDHVACEAVNQEVLFRVAGLPMVENCMAGYNSCIFAYGQTGSGKTYTMLGEISELEV 313
Query: 294 KLNEDCGLTLRIFEHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQ 353
+ ++D G+T RIFE LF RI EE+S+RD KLKY CKCSFLEIYNEQI DLL+PSSTNL
Sbjct: 314 RPSQDRGMTPRIFEFLFARIRAEEESRRDEKLKYNCKCSFLEIYNEQITDLLDPSSTNLP 373
Query: 354 LREDLKKGVYVENLTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIES 413
LRED++ GVYVENLTE V V+D++KLL+QG+ANRK+AAT+MN ESSRSHSVFTCIIES
Sbjct: 374 LREDIRNGVYVENLTELEVGCVSDIIKLLMQGSANRKVAATNMNRESSRSHSVFTCIIES 433
Query: 414 HWEKDSRTHFRFARLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLA 473
WEKDS ++ RFARLNLVDLAGSERQ++SGA G+RLKEAANINKSLST+GLVIMSLVD A
Sbjct: 434 RWEKDSASNLRFARLNLVDLAGSERQRTSGAAGERLKEAANINKSLSTLGLVIMSLVDQA 493
Query: 474 HGKHRHIPYRDSRLTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNN 533
HGK RH+PYRDSRLTFLLQDSLGGNSKT +IANVSPS CSA+ETLSTLKFAQRA+ IQNN
Sbjct: 494 HGKQRHVPYRDSRLTFLLQDSLGGNSKTMIIANVSPSVCSASETLSTLKFAQRARLIQNN 553
Query: 534 AKVNECASSDETALQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVS--------- 593
A VNE AS D ALQ QI LK +L+ +LK PRS+ FE SGV
Sbjct: 554 AVVNEDASGDVLALQHQIRLLKEELA-VLKRQRVPRSLSFTSDIFERSGVDVDDGTESMN 613
Query: 594 -DLFKDYDPLGGR----MQTANHKMKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLA 653
D D D R ++ +N +++L+E TL GA RRE VA +++LE EIE + R+
Sbjct: 614 MDEENDNDAHDRRSLQDLRISNKQLRLLEETLAGAFRRESVAEATVKQLEAEIEQLNRMV 673
Query: 654 FQQEEDAQRTKMLLKFREEKIRQLELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSP 713
+++E D + KM LKFRE+KI Q+E L+ L A+ YLLEEN L+ EI +L+AKI+++P
Sbjct: 674 YERENDTRSAKMTLKFREDKIHQMEALVRDKLPAESYLLEENNTLLKEIDLLRAKIDKNP 733
Query: 714 ELTRVSLENSELIEQLQVFQNFYELGEREALLTEVAELRNELLVALGKNSTISERDKY-- 773
E+TR +LEN L +L+ + F GERE LL EV+ LRN++L L + + + + +
Sbjct: 734 EVTRFALENIRLSNKLKSYNQFCNEGEREHLLNEVSILRNQVLQILERRAEAEQPNNFPT 793
Query: 774 --------QDFHTTKG----LINLENPDTRQIGG--NELQ-----------TDAKKYLKC 833
Q+ T +G + R+I NEL +K+
Sbjct: 794 NFELKRTSQELETCRGELQACLEANKKLAREIADLQNELSNIHSSNREGHPNVVEKFSSA 853
Query: 834 SQGYASVSLSKHREQFGRRDGISMDETMSI-------------KSYSRDDTQSYITGNDE 893
Y S + K + F I+ D+ +++ + +R + + IT D+
Sbjct: 854 LNQYDSHAPEKKDQCFQEGFMINTDDILNLQLELDIIKTILAEERTTRAEVEKRITCLDD 913
Query: 894 RLENEIGQGSDNDFSVKPIC-----SQEDLIDLGMLAETVDSENNMQ-GEDHRCKQVKHD 953
L+ + + C Q +L D + E ++S+ M E K+
Sbjct: 914 ELKAA-------NIHILQTCRQSETMQRELSDARSVIEALESQQIMLINELDELKESNQQ 973
Query: 954 MEENFIKK-LNVTKCQNKGNVVDQHE---------------DVDNKALQVKLENLTKELE 1013
E+ K+ L +++ N+ +V + E + D+ LQ KL+ + LE
Sbjct: 974 SLEHLKKRDLEISRLNNELDVHRRQEFLAMEEPKVQLLKCFENDDSPLQTKLKRMQASLE 1033
Query: 1014 EVRLSNIQYQENQIFQLSQQNQIEHVRQQVEMETANTILQLQEEVETLQL---------- 1073
+ R N +YQ +Q S Q +++ V +QVE+ETA I+ LQEE+ +LQ
Sbjct: 1034 KARKLNTRYQRDQASHSSAQQEMDEVSRQVEVETAEVIMCLQEELISLQQQLDASSKNEL 1093
Query: 1074 --------------ELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMIELTSFLL 1133
+LNDRL + +EN + K++++ ML DW+ ++ +FLL
Sbjct: 1094 LANQRIDEARLEREQLNDRLLEVMKENECFSALIEEKDKKIGMLTNDWDKLASDIGNFLL 1153
Query: 1134 DSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLEDARLMVQ 1193
D + ++ +A Q+ IS + I +QVQ+ +++E + L L++A +
Sbjct: 1154 DGNAALDEASDQVAFISESISQ-RKWIEDQVQKMCRGISQRDELLKELQSRLKEADDIRC 1213
Query: 1194 EMELKLDSLKEATLALN--------------------ESQQMYDNKCAADAKQLSHLLTD 1253
+++LKL SL+ A A+N ES + Y N+ Q LL D
Sbjct: 1214 DLDLKLRSLRGAMQAINDTHQQEKNDQENAMSVLRSQESNERYVNQQQLQELQRIQLLLD 1273
Query: 1254 KNI----------------------MVEFLDKRLNVKKDQLVE----------------- 1313
++I ++ L+ +L+ K L +
Sbjct: 1274 ESIESFVQKEVIEQSYISLQRAMEEVIHHLESQLDQSKRDLTQLLSETQDKEQAFERLKN 1333
Query: 1314 ----------------------------ASLSQWLSYSQELACCNS-------------- 1373
A S + +E+ C NS
Sbjct: 1334 EENGVLLTVLSDVLKAKGVIHEFETGFNAIQSSFSVDPEEVVCQNSDLNLEDRVGCDPTG 1393
Query: 1374 ---------------IGREMPI--------------------SKLDVSFQSSHIIDDVMA 1433
+ +EM + + FQS I DV A
Sbjct: 1394 AFEAGEKHNGDVLCKLSKEMECVVYTMQMLQSQMVKLLQEKENAKEYHFQSQRTIKDVSA 1453
Query: 1434 NTNALLLEELDTESDL------ICLGIMELKNITSAHYADMEMHISALYIYIHDL----- 1493
L E +D E + + + E + T+ + AL + + +
Sbjct: 1454 KVLQLKSEIIDKEKGYEARLKELEIKMQEKEKDTAESFISWNKEREALELEVSEAKSLAI 1513
Query: 1494 ------------YSEYHGLVQD---MVREIHELRLKAKTTNENCKRLQ--FFKDK----- 1553
+ E + D V + E KAK +N K + F +K
Sbjct: 1514 QKSFEASTLISKFEEAQATISDADTTVNALVEANEKAKLQIQNFKENEALFLSEKERLLT 1573
Query: 1554 -VPSAHKYWNIENQ--NSMLDQIKAKIYEAKNRLNILEDSID--RNIAGCGYQYIGQ--- 1613
+ S ++++Q M + A + EA + LED I +N+ +++
Sbjct: 1574 EISSLKMLLDVKDQTYEVMEKKFAASLLEANDLALELEDGIRHLQNLLLEKLEFVSSDVE 1633
Query: 1614 -------------YPVNEDGW----SSDCSTS-----SSDISTESVTSRGKLNGLLCSHF 1673
E+ W DC+ S I E +T NG L
Sbjct: 1634 WMKSKLQQFAELARTWLEENWLEIIGKDCAVSVLHLCHMGILLERITGLNAENGFL--QR 1693
Query: 1674 NSLEGDSVI-------------CELCKKVESVKLLDSMNGDS------GTTTCLRRELCM 1733
E +S+I E+C ++ LLD + S T L L
Sbjct: 1694 GLCESNSLISKLREHNDRAKNELEMCSVLKGKLLLDINHNFSRIAKKEQEATELNSRLDA 1753
Query: 1734 TYNAIHKLSMQIDAF-----QMYN--------IGGDSLSEEMDQAKTTPKLRMGNAEA-G 1793
I L Q +A MYN I + S ++K +LR EA
Sbjct: 1754 FEKKILHLQAQEEAMLARSNSMYNELSILVEEIDATNKSALATESKEKEELRHQLDEALL 1813
Query: 1794 CNNISKVISIKEI--KQDGGFLTRFEEAQAAIKEADIMLNALLRANENAKQ-----LTGI 1853
CN + K I +++ Q ++ E + + AD N L+ N AK L+
Sbjct: 1814 CNAMLKDIIQEDVDLPQVNNYMKGCSEFELCNRLADYH-NELVTTNIIAKDIESFVLSSE 1873
Query: 1854 FKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFNLEEVANLVSSLEGCISQ 1913
Q QLQ + I+ + L + L D K L V F+L + +N + + Q
Sbjct: 1874 LVQQKAQLQKQELMFIDALDGLTTEATLSRVD-KDLGSAVIFSLLDDSNKIMIDFDNLKQ 1933
Query: 1914 TQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIGREFASFVLHECYVKEVC 1973
+ E+ E ++S + ++ R+ + + +S +E + E+ G+ A L Y
Sbjct: 1934 NKDELMENLHVLSEENLNLRS----VVGSLESSIESLQTELDGKTKALMELQ--YSHTTI 1993
Query: 1974 CQFARFKAEPSFQLLKQQRCMESNNN---SRSTCIADKDDMM--LISKID--------RG 2033
+ + K++ + + ++ + S NN I K+ MM L++ +D +G
Sbjct: 1994 LEEFKLKSKATELGVSRENDLRSENNLLKHEYLDIVRKEQMMAELVANLDSEKLFVTIQG 2053
Query: 2034 RSELVTG----------------LEEAD-AGFSYDDILYENLALKKELKRKEVLLEGLLF 2093
R E V E D S + ++ N L+ EL RK+ L +GL F
Sbjct: 2054 RLEQVADQVQMYTSDQLNMVTKVSNEIDFIQMSIEGLITHNGFLQSELIRKDELAKGLSF 2113
Query: 2094 DFRLLQESTSKTKDMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEG-LLTDTEK 2153
D LLQES S KD D+ +L ++ LE EL+ K+++L + + ++ LE +L +K
Sbjct: 2114 DLSLLQESASVAKDQADELIQLTEAIESLEPELDSKSNELVDAVSGRQLLEAQILKSNQK 2173
Query: 2154 ALFLSN---SKLDKAKETMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENE 2213
L SK+++ KE +S +L ++ + + +LAD+ + RLE E
Sbjct: 2174 VSALEEQLASKINELKE----VSVEKDELTSKLNHIEGISYTMEDELADKSKAIERLEEE 2233
Query: 2214 ILHRTSSVEKKLLLSVEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIE 2273
++ S ++ + +++++N+ +++ E+ QV L +KL++A ALA+E EA+A E
Sbjct: 2234 LIELRSLLDARTCF-LQNLQNDFSKLLDEKKYCETQVLILNEKLEMAQALAEESEAIATE 2293
Query: 2274 ARQESEASKLYAEQKEEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELE 2307
A+Q +E K +AE+K+EEVK+LE S+EELE+T+ LE KV + EE E+ RE +ELE
Sbjct: 2294 AKQMAEERKTHAEEKDEEVKLLERSIEELETTVCALENKVDIIKEEAERQRMHREEIELE 2353
BLAST of Sgr025536 vs. ExPASy Swiss-Prot
Match:
Q27IK6 (Kinesin-like protein KIN-12D OS=Arabidopsis thaliana OX=3702 GN=KIN12D PE=2 SV=1)
HSP 1 Score: 979.2 bits (2530), Expect = 8.2e-284
Identity = 856/2597 (32.96%), Postives = 1279/2597 (49.25%), Query Frame = 0
Query: 91 NACTNNGTPKFSAQGRWVNSEPSSTHSTPARSSSR---VSFGGAIVATGSKAPQLADGRA 150
NA N TPK SE +ST +TP +S S+ + G + TG+ RA
Sbjct: 99 NAALLNTTPKTGRVVGRAYSETNSTQNTPTKSVSKPPGSCYRGKLDGTGTV-------RA 158
Query: 151 GSSFRLSRRISIPNTEL-----PVEVPHFDLAEDPSFWNDHNVQVMIRIRPLSTTERDSQ 210
G L + +S + ++ VEVPHF L EDPSFW DHNVQ++IR+RPL++ ER
Sbjct: 159 GGYASLYKGLSSSSGQVSTVVNSVEVPHFSLKEDPSFWMDHNVQILIRVRPLNSMERSIN 218
Query: 211 GYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQENLFKVAGQPMVENCLSGYNSCMF 270
GY RCL+QE+++ + WIG PETRF FDH+AC+TI QE LF+VAG PMVENCLSGYNSC+F
Sbjct: 219 GYNRCLKQESSQCVAWIGPPETRFQFDHVACETIDQETLFRVAGLPMVENCLSGYNSCIF 278
Query: 271 AYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEHLFTRIGMEEKSKRDAKLKYCCKC 330
AYGQTGSGKTYTM+G + ++E K + + G+ RIFE LF RI EE+S+RD +LKY CKC
Sbjct: 279 AYGQTGSGKTYTMLGEVGDLEFKPSPNRGMMPRIFEFLFARIQAEEESRRDERLKYNCKC 338
Query: 331 SFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLTEHNVTTVNDVVKLLLQGAANRKM 390
SFLEIYNEQI DLLEPSSTNLQLRED+K GVYVENLTE V +V D++ L+ QG+ NR++
Sbjct: 339 SFLEIYNEQITDLLEPSSTNLQLREDIKSGVYVENLTECEVQSVQDILGLITQGSLNRRV 398
Query: 391 AATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARLNLVDLAGSERQKSSGAEGDRLKE 450
AT+MN ESSRSHSVFTC+IES WEKDS + RFARLNLVDLAGSERQK+SGAEGDRLKE
Sbjct: 399 GATNMNRESSRSHSVFTCVIESRWEKDSTANMRFARLNLVDLAGSERQKTSGAEGDRLKE 458
Query: 451 AANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLGGNSKTTVIANVSPSF 510
AA+INKSLST+G VIM LVD+A+GK RHIPYRDSRLTFLLQDSLGGNSKT +IAN SPS
Sbjct: 459 AASINKSLSTLGHVIMVLVDVANGKPRHIPYRDSRLTFLLQDSLGGNSKTMIIANASPSV 518
Query: 511 CSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQRQILHLKGQLSFLLKHSNFPRSI 570
A ETL+TLKFAQRAK IQNNA VNE ++ D L+RQI LK +LS LLK N R++
Sbjct: 519 SCAAETLNTLKFAQRAKLIQNNAVVNEDSNEDVLELRRQIRLLKEELS-LLKRQNISRAL 578
Query: 571 M--SNVQRFEESGV---SDLFKD-----------YDPLGGRMQTANHKMKLMEATLIGAL 630
S F ES V S + + Y+ GG ++ + ++K +E TL G+L
Sbjct: 579 SFGSATANFAESQVDSPSSVMHETGQQQAGNLLVYES-GGCVRMSRKQLKSLEITLAGSL 638
Query: 631 RREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLGGMLSADK 690
RRE VA+ +I+KLE EIEH+ RL Q+EED + TKM+L+FRE+KI++LE LLG +SAD
Sbjct: 639 RREHVADASIKKLEAEIEHLNRLVRQREEDTRSTKMMLRFREDKIQRLESLLGNHISADS 698
Query: 691 YLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGEREALLTEVA 750
+LLEEN L EI++LQAKI+++PELTR +LEN L++QL+ FQ FYE GERE LL EV+
Sbjct: 699 FLLEENNVLSEEIQLLQAKIDKNPELTRFALENIRLLDQLRRFQEFYEEGEREILLGEVS 758
Query: 751 ELRNELLVALGKNS-----------------------TISERDKYQDFHTTKGLINL--- 810
LRN+L L +NS ++ E K + K NL
Sbjct: 759 NLRNQLFQFLDENSDWQKHVDDGIEPQGASRMSKENCSLQEELKKTCYELEKCRSNLGSC 818
Query: 811 --ENPD-TRQIGGNELQTDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMS----- 870
EN +R+I N+LQ C+ S S++K + G ++ ET++
Sbjct: 819 LEENAKLSREI--NDLQAMVSDIRACTPDEHS-SVNKQKALLGTQN-FEPHETLACEQAN 878
Query: 871 -----IKSYSRDDTQSYITGNDERLENEI-GQGSDNDFSVKPICSQEDLID------LGM 930
IK D Q I + L + Q F ++ + Q LI
Sbjct: 879 YVEEIIKLQLDLDVQKIILDEERTLRGDTEAQAVRLKFDIEVLKDQLLLISKQQKNVYSE 938
Query: 931 LAET------VDSEN-NMQGEDHRCKQVKHDMEENFIKKLNVTKCQNKGNVVDQHED--V 990
L ET ++S+N + E +++K + E +KK + K D+ +D
Sbjct: 939 LGETKSAVAALESQNIILIQEAVELRRIKENYFE-LLKKQELDIPAMKSKQCDEFKDNPA 998
Query: 991 DNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQVEMETANTILQLQE 1050
++ + K + + LE+ + N+ Y+ + + +++ V +Q E TA I+ LQ
Sbjct: 999 EDSEIDTKFKKMQASLEKAKRLNMLYKSDIASKACGDEEMDEVCKQAEAATAEVIVCLQN 1058
Query: 1051 EVETLQLELN------------------------DRLHGLAQENTRLKDALSAKNEEMRM 1110
E+E LQ E+N D+L +N +L++ L K+ E+ +
Sbjct: 1059 ELEVLQKEVNDFQSKENVTEKQVEILETQMEELQDKLRDTTMDNEQLQEQLRGKDMELLI 1118
Query: 1111 LCVDWETAMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEE 1170
+ + E EL LL+ + + DA Q + IS P + ISEQV I E+E
Sbjct: 1119 ISNEMELLTSELEEILLNGNEGLTDACYQADLISGSLPDKRIWISEQVGGLIRTLSEREL 1178
Query: 1171 TILLLHKSLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNI 1230
I L LEDA ++E L SLK A + +NE+ Q + D L L K
Sbjct: 1179 MIEDLESCLEDANKKRCDIESMLKSLKGAAIVMNEAHQREFEEKETDVLLLKSQLCTKTE 1238
Query: 1231 MVEFLDKRLNVKKDQLVEASLSQWLS------YSQ------------ELACCNSIGR--- 1290
+ L ++L + + + EAS S YS+ + S G
Sbjct: 1239 TILRLQEKLKMAERLIYEASDCATASLIIVNRYSEVTESHTFELKQKDFQVAESTGTILS 1298
Query: 1291 -EMPISKLDVS---FQSSHIIDDVMANTNALLLEEL-DTESDLICLGIMELKNITSAHYA 1350
+ + L+ + F+S + ++ A+ LEE+ +T + + ELK S
Sbjct: 1299 LKQQVQDLEATCKEFRSKLLEEEKNASAMEQKLEEIEETSISAMKEKLSELKGGVS---- 1358
Query: 1351 DMEMHISALYIYIHDLYSEYHGLV---------QDMVREI---------------HELRL 1410
++ HD Y+E + Q+ R + +RL
Sbjct: 1359 --DLRSCITMCQEHDKYTEAENSLSSPAHCSEGQEPGRNVVVSSCIEKTPNNNHTESMRL 1418
Query: 1411 KAKTTNENCKRLQFFKDKVPSA---HKYWNIENQN----------------SMLDQIKAK 1470
+K ++E K + K ++ SA K +E N S L+ + A+
Sbjct: 1419 SSKVSSERGKVIILLKQEMESALASLKEVQVEMANLKGEKEELKASEKRSLSNLNDLAAQ 1478
Query: 1471 I-------------YEAK-----NRLNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCS 1530
I YE K ++L LE I + +Y+ + + + +
Sbjct: 1479 ICNLNTVMSNMEEQYEHKMEVTDHKLKTLEHEIAKMKIEADQEYVENLCILKKFEEAQGT 1538
Query: 1531 TSSSDIS-TESVTSRGKLNGLLCSHFNSLEGDSVICELCKKVESVKLLDSMNGDSGTTTC 1590
+DI+ E V + K+ L G S++ E VE ++ L+S+N
Sbjct: 1539 IREADITVNELVIANEKMRFDL--EKQKKRGISLVGEKKALVEKLQELESINVKENEKLA 1598
Query: 1591 LRRELCMTY-----NAIHKLSMQIDAFQ-MYNIGGDSLSEEMDQAKT----TPKLRMGNA 1650
+L + N + +L+ + Q ++ +++++ + K+ T R+
Sbjct: 1599 YLEKLFESSLMGIGNLVEELATVVRKLQDESSVALTGMAKDLSELKSWVSETNSARLFLE 1658
Query: 1651 EAGCNNISK---------------VISIKEIKQDGGFLTR----FEEAQAAIKEADIMLN 1710
+ I K + ++ I + G L R + A +++ ++ L
Sbjct: 1659 DIWSEIIMKDCAISVLHLCHMGILLETVTGINTENGLLQRGLCVSNSSIAGLRDNNLRLR 1718
Query: 1711 ALLR--ANENAKQLTGI-----------------------FKQAGEQLQIERDNLIEEVG 1770
L AN K LT I F Q LQ + D +++
Sbjct: 1719 RELEMFANLKGKLLTDIKNGFERISRNEEATNLLTTKLSSFDQKISGLQYQEDLMLQRSN 1778
Query: 1771 QLKSSMH--LKDAD-------NKLLQEQVWFN-----------------------LEEVA 1830
+ S + LK+ D LL+++ N L +A
Sbjct: 1779 SMGSQLDILLKEIDLSNGDLAETLLEQERHLNQKNDFFDTEVQLYLMDLCSKDVELLVLA 1838
Query: 1831 NLVSSLEGCISQTQREVDEKFGIM------------------------SCDVISFRNEML 1890
C++ RE+ + I+ + +S + E+
Sbjct: 1839 QTAKEYSSCLAVVDRELLDHHVIVEDLKEKLIVSQVEGELKDQCLVDNKLETVSVKEELT 1898
Query: 1891 KSISNWKSLMEDIC--------LEIIGREFASFVL------------------------- 1950
++ S K L D+ ++ + ++F V+
Sbjct: 1899 EAQSKIKVLSSDLDRSVQKIAEIDEVNKDFGERVIFLESSITGLQQELAMKASELYSLEH 1958
Query: 1951 ------HECYVKEVCCQ-------------------FARFKAEPSFQLLKQQR-----CM 2010
E +KE Q F F E F+ L R C
Sbjct: 1959 SRSVTAEELDIKERDVQVYADIVSSLKKENVSLKNKFIHF-GEDQFKALDVTRLSIAKCS 2018
Query: 2011 ESNNNSR--------STCIADKDDMMLISKIDRGR--SELVTGLEEADAGFSYDDILYEN 2070
+S+ I+DK ++ +D+ ++ V L+ ++L EN
Sbjct: 2019 HLTEDSKKLEKLTRDGMAISDKMLQLICENVDKASVFADTVQSLQ-----IDVQELLSEN 2078
Query: 2071 LALKKELKRKEVLLEGLLFDFRLLQESTSKTKDMKDQTEKLDYSLSQLEHELEIKASQLE 2130
L L EL RK+ +L+GL FD LLQES S ++D KD+T+++ + LE L +K +LE
Sbjct: 2079 LNLHDELLRKDDVLKGLSFDLSLLQESASNSRDKKDETKEIMVHVEALEKTLALKTFELE 2138
Query: 2131 NVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMTIISEHNAQLKKQVEDLHLKKSEAKK 2190
+ + + LE L ++++ +KA++ +S N ++ + EDL +K ++
Sbjct: 2139 DAVSHAQMLEVRLQESKEITRNLEVDTEKARKCQEKLSAENKDIRAEAEDLLAEKCSLEE 2198
Query: 2191 QLADQQDVVNRLENEILHRTSSVEKKLLLSVEDIENELRRVISERDQLREQVCFLTDKLD 2250
++ + V +E E+ + +++ +L +V + +L I ERD L+++V L ++
Sbjct: 2199 EMIQTKKVSESMEMELFNLRNAL-GQLNDTVAFTQRKLNDAIDERDNLQDEVLNLKEEFG 2258
Query: 2251 IAYALADEKEAVAIEARQESEASKLYAEQKEEEVKILEHSVEELESTINMLETKVHEMDE 2305
+ A E EA IEA+Q +E+ K YA+++EEEVK+LE SVEELE TIN+LE KV+ + +
Sbjct: 2259 KMKSEAKEMEARYIEAQQIAESRKTYADEREEEVKLLEGSVEELEYTINVLENKVNVVKD 2318
BLAST of Sgr025536 vs. ExPASy Swiss-Prot
Match:
Q75LL2 (Kinesin-like protein KIN-12G OS=Oryza sativa subsp. japonica OX=39947 GN=KIN12G PE=3 SV=1)
HSP 1 Score: 589.3 bits (1518), Expect = 1.8e-166
Identity = 308/571 (53.94%), Postives = 416/571 (72.85%), Query Frame = 0
Query: 171 FDLAEDPSFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDH 230
F+L EDPSFW D+NVQV+IR+RPLS+ E QG RC+RQ++ +++ W GHPE+RF FD
Sbjct: 19 FELQEDPSFWKDNNVQVVIRVRPLSSGEISVQGQKRCVRQDSCQSITWTGHPESRFKFDL 78
Query: 231 IACDTISQENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDC 290
+A + ++QENLFKVAG PMV+NC++GYNSCMFAYGQTGSGKT+TM+G I + N +C
Sbjct: 79 VADEYVTQENLFKVAGVPMVDNCMAGYNSCMFAYGQTGSGKTHTMLGDIENGTRRNNVNC 138
Query: 291 GLTLRIFEHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLK 350
G+T R+FEHLF RI E++ +++ KL++ CKCSFLEIYNEQI DLL P+S NLQ+RED K
Sbjct: 139 GMTPRVFEHLFLRIQKEKEIRKEEKLRFTCKCSFLEIYNEQILDLLNPNSVNLQIREDAK 198
Query: 351 KGVYVENLTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDS 410
KGV+VENLTEH V+ + ++ L++GAANRK+AAT+MN SSRSHSVFTC+IES WE
Sbjct: 199 KGVHVENLTEHEVSNAREAMQQLVEGAANRKVAATNMNRASSRSHSVFTCLIESKWESQG 258
Query: 411 RTHFRFARLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRH 470
H RF+RLNLVDLAGSERQKSSGAEG+RLKEA NINKSLST+GLVI +L+ +++ K H
Sbjct: 259 INHHRFSRLNLVDLAGSERQKSSGAEGERLKEATNINKSLSTLGLVITNLIAVSNKKSHH 318
Query: 471 IPYRDSRLTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNEC 530
+PYRDS+LTFLLQDSLGGNSKTT+IAN+SPS C A ETLSTLKFAQRAK I+NNA +NE
Sbjct: 319 VPYRDSKLTFLLQDSLGGNSKTTIIANISPSSCCAAETLSTLKFAQRAKYIRNNAIINED 378
Query: 531 ASSDETALQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDL-----FKDYDPLG 590
AS D +++ QI HLK ++S L N ++ ++ F S L + PL
Sbjct: 379 ASGDVLSMRLQIQHLKKEVSRLQGLVNSDKAECTSSSGFICESPSTLKWNQGQGSFSPL- 438
Query: 591 GRMQTANHKMKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLK 650
+ K +A L+ A RRE+ ++ + ++LA Q+ E+ + KM L+
Sbjct: 439 -MFDKRAMQRKDYDAALVAAFRREQETEAKLKAMIAAKLVAEQLATQRAEEVRSFKMRLR 498
Query: 651 FREEKIRQLELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQ 710
FRE++I++LE + G LSA+ +LL+EN+ LV E+ L+ ++R+PE+TR ++EN +L E
Sbjct: 499 FREDRIKRLEQVTSGKLSAESHLLQENEDLVKEVDALRGLLDRNPEVTRFAMENLQLKED 558
Query: 711 LQVFQNFYELGEREALLTEVAELRNELLVAL 737
++ Q F + GERE + ++ L+++LL AL
Sbjct: 559 IRRLQTFVDEGEREMMHEQIIVLQDKLLEAL 587
BLAST of Sgr025536 vs. ExPASy TrEMBL
Match:
A0A6J1CB46 (kinesin-like protein KIN-12C isoform X3 OS=Momordica charantia OX=3673 GN=LOC111009617 PE=3 SV=1)
HSP 1 Score: 3563.9 bits (9240), Expect = 0.0e+00
Identity = 1917/2329 (82.31%), Postives = 2049/2329 (87.98%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKL 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHKMKL
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMKL 600
Query: 601 MEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
MEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL
Sbjct: 601 MEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
Query: 661 LGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGE 720
LGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+LGE
Sbjct: 661 LGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYDLGE 720
Query: 721 REALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDA 780
REALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 REALLTEVAELRNELLVALGKNSTVSERDKYQ---------------------------- 780
Query: 781 KKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIG 840
DETMSIKSYSRDDT SYIT N E LENE G
Sbjct: 781 ------------------------------DETMSIKSYSRDDTLSYITENAENLENETG 840
Query: 841 QGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTK 900
QGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ NVTK
Sbjct: 841 QGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFNVTK 900
Query: 901 CQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQ 960
QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHVRQQ
Sbjct: 901 RQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHVRQQ 960
Query: 961 VEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMI 1020
VEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWETAMI
Sbjct: 961 VEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWETAMI 1020
Query: 1021 ELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLE 1080
ELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHKSLE
Sbjct: 1021 ELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHKSLE 1080
Query: 1081 DARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLN 1140
DARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDKR
Sbjct: 1081 DARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDKRFK 1140
Query: 1141 VKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDDVMA 1200
+KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD MA
Sbjct: 1141 FQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDDAMA 1200
Query: 1201 NTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDM 1260
TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LVQ+M
Sbjct: 1201 KTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELVQEM 1260
Query: 1261 VREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNI 1320
V+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNRLN+
Sbjct: 1261 VKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNRLNV 1320
Query: 1321 LEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSL 1380
LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY-------- 1380
Query: 1381 EGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGG 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++IGG
Sbjct: 1381 ---------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHDIGG 1440
Query: 1441 DSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIM 1500
D LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEAD+M
Sbjct: 1441 DPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEADLM 1500
Query: 1501 LNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFN 1560
LNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQVWF+
Sbjct: 1501 LNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQVWFS 1560
Query: 1561 LEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIG 1620
LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LEI G
Sbjct: 1561 LEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLEITG 1620
Query: 1621 REFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISK 1680
REFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L SK
Sbjct: 1621 REFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMILTSK 1680
Query: 1681 IDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTK 1740
ID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+KTK
Sbjct: 1681 IDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTAKTK 1740
Query: 1741 DMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKE 1800
DMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+KAKE
Sbjct: 1741 DMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEKAKE 1800
Query: 1801 TMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKLLLS 1860
TM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKLLLS
Sbjct: 1801 TMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKLLLS 1860
Query: 1861 VEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQK 1920
VEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYAEQK
Sbjct: 1861 VEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYAEQK 1920
Query: 1921 EEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLT 1980
EEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV NLT
Sbjct: 1921 EEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVGNLT 1980
Query: 1981 EVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVL 2040
E VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI ELVL
Sbjct: 1981 EAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILELVL 2040
Query: 2041 HADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCISNLV 2100
HAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCISNLV
Sbjct: 2041 HADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCISNLV 2100
Query: 2101 HQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
HQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL
Sbjct: 2101 HQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
Query: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAE 2220
TKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKKEAE
Sbjct: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKKEAE 2220
Query: 2221 ALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESRHAF 2280
AL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++ HAF
Sbjct: 2221 ALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNHHAF 2228
Query: 2281 LTK-QDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
LTK QDDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 LTKVQDDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2228
BLAST of Sgr025536 vs. ExPASy TrEMBL
Match:
A0A6J1CD04 (kinesin-like protein KIN-12C isoform X4 OS=Momordica charantia OX=3673 GN=LOC111009617 PE=3 SV=1)
HSP 1 Score: 3561.9 bits (9235), Expect = 0.0e+00
Identity = 1916/2328 (82.30%), Postives = 2048/2328 (87.97%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKL 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHKMKL
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMKL 600
Query: 601 MEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
MEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL
Sbjct: 601 MEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELL 660
Query: 661 LGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGE 720
LGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+LGE
Sbjct: 661 LGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYDLGE 720
Query: 721 REALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDA 780
REALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 REALLTEVAELRNELLVALGKNSTVSERDKYQ---------------------------- 780
Query: 781 KKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIG 840
DETMSIKSYSRDDT SYIT N E LENE G
Sbjct: 781 ------------------------------DETMSIKSYSRDDTLSYITENAENLENETG 840
Query: 841 QGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTK 900
QGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ NVTK
Sbjct: 841 QGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFNVTK 900
Query: 901 CQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQ 960
QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHVRQQ
Sbjct: 901 RQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHVRQQ 960
Query: 961 VEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMI 1020
VEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWETAMI
Sbjct: 961 VEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWETAMI 1020
Query: 1021 ELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLE 1080
ELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHKSLE
Sbjct: 1021 ELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHKSLE 1080
Query: 1081 DARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLN 1140
DARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDKR
Sbjct: 1081 DARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDKRFK 1140
Query: 1141 VKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDDVMA 1200
+KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD MA
Sbjct: 1141 FQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDDAMA 1200
Query: 1201 NTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDM 1260
TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LVQ+M
Sbjct: 1201 KTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELVQEM 1260
Query: 1261 VREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNI 1320
V+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNRLN+
Sbjct: 1261 VKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNRLNV 1320
Query: 1321 LEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSL 1380
LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY-------- 1380
Query: 1381 EGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGG 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++IGG
Sbjct: 1381 ---------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHDIGG 1440
Query: 1441 DSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIM 1500
D LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEAD+M
Sbjct: 1441 DPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEADLM 1500
Query: 1501 LNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFN 1560
LNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQVWF+
Sbjct: 1501 LNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQVWFS 1560
Query: 1561 LEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIG 1620
LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LEI G
Sbjct: 1561 LEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLEITG 1620
Query: 1621 REFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISK 1680
REFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L SK
Sbjct: 1621 REFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMILTSK 1680
Query: 1681 IDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTK 1740
ID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+KTK
Sbjct: 1681 IDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTAKTK 1740
Query: 1741 DMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKE 1800
DMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+KAKE
Sbjct: 1741 DMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEKAKE 1800
Query: 1801 TMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKLLLS 1860
TM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKLLLS
Sbjct: 1801 TMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKLLLS 1860
Query: 1861 VEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQK 1920
VEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYAEQK
Sbjct: 1861 VEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYAEQK 1920
Query: 1921 EEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLT 1980
EEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV NLT
Sbjct: 1921 EEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVGNLT 1980
Query: 1981 EVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVL 2040
E VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI ELVL
Sbjct: 1981 EAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILELVL 2040
Query: 2041 HADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCISNLV 2100
HAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCISNLV
Sbjct: 2041 HADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCISNLV 2100
Query: 2101 HQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
HQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL
Sbjct: 2101 HQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDL 2160
Query: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAE 2220
TKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKKEAE
Sbjct: 2161 TKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKKEAE 2220
Query: 2221 ALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESRHAF 2280
AL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++ HAF
Sbjct: 2221 ALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNHHAF 2226
Query: 2281 LTKQDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
LTK DDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 LTK-DDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2226
BLAST of Sgr025536 vs. ExPASy TrEMBL
Match:
A0A6J1C9G8 (kinesin-like protein KIN-12C isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009617 PE=3 SV=1)
HSP 1 Score: 3558.5 bits (9226), Expect = 0.0e+00
Identity = 1917/2332 (82.20%), Postives = 2049/2332 (87.86%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHK--- 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHK
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMQQ 600
Query: 601 MKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
MKLMEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL
Sbjct: 601 MKLMEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
Query: 661 ELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYE 720
ELLLGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+
Sbjct: 661 ELLLGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYD 720
Query: 721 LGEREALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQ 780
LGEREALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 LGEREALLTEVAELRNELLVALGKNSTVSERDKYQ------------------------- 780
Query: 781 TDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLEN 840
DETMSIKSYSRDDT SYIT N E LEN
Sbjct: 781 ---------------------------------DETMSIKSYSRDDTLSYITENAENLEN 840
Query: 841 EIGQGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLN 900
E GQGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ N
Sbjct: 841 ETGQGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFN 900
Query: 901 VTKCQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHV 960
VTK QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHV
Sbjct: 901 VTKRQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHV 960
Query: 961 RQQVEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWET 1020
RQQVEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWET
Sbjct: 961 RQQVEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWET 1020
Query: 1021 AMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHK 1080
AMIELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHK
Sbjct: 1021 AMIELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHK 1080
Query: 1081 SLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDK 1140
SLEDARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDK
Sbjct: 1081 SLEDARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDK 1140
Query: 1141 RLNVKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDD 1200
R +KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD
Sbjct: 1141 RFKFQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDD 1200
Query: 1201 VMANTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLV 1260
MA TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LV
Sbjct: 1201 AMAKTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELV 1260
Query: 1261 QDMVREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNR 1320
Q+MV+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNR
Sbjct: 1261 QEMVKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNR 1320
Query: 1321 LNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHF 1380
LN+LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LNVLEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY----- 1380
Query: 1381 NSLEGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYN 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++
Sbjct: 1381 ------------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHD 1440
Query: 1441 IGGDSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEA 1500
IGGD LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEA
Sbjct: 1441 IGGDPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEA 1500
Query: 1501 DIMLNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQV 1560
D+MLNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQV
Sbjct: 1501 DLMLNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQV 1560
Query: 1561 WFNLEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLE 1620
WF+LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LE
Sbjct: 1561 WFSLEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLE 1620
Query: 1621 IIGREFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMML 1680
I GREFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L
Sbjct: 1621 ITGREFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMIL 1680
Query: 1681 ISKIDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTS 1740
SKID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+
Sbjct: 1681 TSKIDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTA 1740
Query: 1741 KTKDMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDK 1800
KTKDMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+K
Sbjct: 1741 KTKDMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEK 1800
Query: 1801 AKETMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKL 1860
AKETM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKL
Sbjct: 1801 AKETMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKL 1860
Query: 1861 LLSVEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYA 1920
LLSVEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYA
Sbjct: 1861 LLSVEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYA 1920
Query: 1921 EQKEEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVE 1980
EQKEEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV
Sbjct: 1921 EQKEEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVG 1980
Query: 1981 NLTEVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISE 2040
NLTE VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI E
Sbjct: 1981 NLTEAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILE 2040
Query: 2041 LVLHADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCIS 2100
LVLHAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCIS
Sbjct: 2041 LVLHADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCIS 2100
Query: 2101 NLVHQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
NLVHQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK
Sbjct: 2101 NLVHQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
Query: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKK 2220
LDLTKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKK
Sbjct: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKK 2220
Query: 2221 EAEALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESR 2280
EAEAL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++
Sbjct: 2221 EAEALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNH 2231
Query: 2281 HAFLTK-QDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
HAFLTK QDDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 HAFLTKVQDDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2231
BLAST of Sgr025536 vs. ExPASy TrEMBL
Match:
A0A6J1C9Q8 (kinesin-like protein KIN-12C isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009617 PE=3 SV=1)
HSP 1 Score: 3556.5 bits (9221), Expect = 0.0e+00
Identity = 1916/2331 (82.20%), Postives = 2048/2331 (87.86%), Query Frame = 0
Query: 1 MSKQISASLSTYLDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQ 60
MSKQ+S SLS QPE NEN FGTSPSALHFPPPRTPFNIIADPAQY KE Q
Sbjct: 1 MSKQVSTSLS-----------QPEPNENEFGTSPSALHFPPPRTPFNIIADPAQYQKEFQ 60
Query: 61 DSGFDSNFKLQSIKADWSSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTP 120
DSGF SN+KLQSIK DWS D +SEVSLK +G+AC NN TP+ SAQGR V+SEPSS +TP
Sbjct: 61 DSGFASNYKLQSIKPDWSCDTKSEVSLKFSGSACINNATPRLSAQGRRVSSEPSSRQTTP 120
Query: 121 ARSSSRVSFGGAIVATGSKAPQLADGRAGSSFRLSRRIS--IPNTELPVEVPHFDLAEDP 180
A+SSSRV FGGAIVA SKA QLADGR GSSFRLSRR+S IPNTELPVEVPHFDL EDP
Sbjct: 121 AKSSSRVPFGGAIVA--SKAHQLADGRGGSSFRLSRRMSMPIPNTELPVEVPHFDLVEDP 180
Query: 181 SFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTIS 240
SFW DHNVQVMIRIRPLSTTERDSQGYGRCLRQ+TAKTLVW+GHPETRFTFDHIAC+TIS
Sbjct: 181 SFWKDHNVQVMIRIRPLSTTERDSQGYGRCLRQDTAKTLVWLGHPETRFTFDHIACETIS 240
Query: 241 QENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIF 300
QE+LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEG+LNEDCGLTLRIF
Sbjct: 241 QESLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGRLNEDCGLTLRIF 300
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
EHLFTRIGMEEKSKRD KLKY CKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN
Sbjct: 301 EHLFTRIGMEEKSKRDVKLKYSCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFA 420
LTEHNVTT+NDVVKLLLQGAANRKMAATHMNS SSRSHSVFTCIIESHWEKDSRTHFRFA
Sbjct: 361 LTEHNVTTINDVVKLLLQGAANRKMAATHMNSXSSRSHSVFTCIIESHWEKDSRTHFRFA 420
Query: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSR 480
RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSR
Sbjct: 421 RLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSR 480
Query: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETA 540
LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETA
Sbjct: 481 LTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETA 540
Query: 541 LQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHK--- 600
LQRQILHLKGQLSFLLKHSNFPRSI+SNV FEESGVSDLF YD LGGRM+TANHK
Sbjct: 541 LQRQILHLKGQLSFLLKHSNFPRSILSNVPIFEESGVSDLFVGYDSLGGRMETANHKMQQ 600
Query: 601 MKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
MKLMEAT IGALRREEVANTAIQKLE EIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL
Sbjct: 601 MKLMEATFIGALRREEVANTAIQKLECEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQL 660
Query: 661 ELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYE 720
ELLLGG+LS DKYLLEENKALVVEI MLQAKINR+PELT++S ENS LIEQLQVFQNFY+
Sbjct: 661 ELLLGGVLSTDKYLLEENKALVVEINMLQAKINRNPELTKISSENSRLIEQLQVFQNFYD 720
Query: 721 LGEREALLTEVAELRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQ 780
LGEREALLTEVAELRNELLVALGKNST+SERDKYQ
Sbjct: 721 LGEREALLTEVAELRNELLVALGKNSTVSERDKYQ------------------------- 780
Query: 781 TDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLEN 840
DETMSIKSYSRDDT SYIT N E LEN
Sbjct: 781 ---------------------------------DETMSIKSYSRDDTLSYITENAENLEN 840
Query: 841 EIGQGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLN 900
E GQGSDN+F+VK ICSQ+DLID LAET+DSENNMQGE++RCKQVKH M F ++ N
Sbjct: 841 ETGQGSDNEFNVKLICSQKDLIDTRTLAETMDSENNMQGENYRCKQVKHGMMGEFFQQFN 900
Query: 901 VTKCQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHV 960
VTK QN+GN+VDQ+EDVDNKALQ+KLENLT+ELEEVR+SNIQYQENQIFQLSQQNQ+EHV
Sbjct: 901 VTKRQNEGNMVDQNEDVDNKALQIKLENLTRELEEVRISNIQYQENQIFQLSQQNQVEHV 960
Query: 961 RQQVEMETANTILQLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWET 1020
RQQVEMETA+TILQLQEEVE LQLELNDRLHGLAQENTRLKDALS K+EEMR+LC+DWET
Sbjct: 961 RQQVEMETASTILQLQEEVEGLQLELNDRLHGLAQENTRLKDALSVKSEEMRVLCIDWET 1020
Query: 1021 AMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHK 1080
AMIELTSFL+DSSRSIRDAHGQIEGI+NLFPKVNVGI EQVQRAIEVC+EKEETILLLHK
Sbjct: 1021 AMIELTSFLVDSSRSIRDAHGQIEGIANLFPKVNVGIKEQVQRAIEVCIEKEETILLLHK 1080
Query: 1081 SLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDK 1140
SLEDARLMVQEMELKL+SLKEATL LNESQQM+DN AADAKQL L TDKN MVEFLDK
Sbjct: 1081 SLEDARLMVQEMELKLNSLKEATLTLNESQQMHDNANAADAKQLRTLTTDKNSMVEFLDK 1140
Query: 1141 RLNVKKDQLVEASLS--------QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDD 1200
R +KD+LV+ S +WLS SQELACCN+IGREMPIS+LDVS Q SH+IDD
Sbjct: 1141 RFKFQKDRLVKTEKSADAAFIAVEWLSNSQELACCNNIGREMPISELDVSSQGGSHVIDD 1200
Query: 1201 VMANTNALLLEELDTESDLICLGIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLV 1260
MA TN LLLEELDTES+LI LGIMEL+NITSAHYADME HISAL I IHDLYSEY LV
Sbjct: 1201 AMAKTNTLLLEELDTESNLIWLGIMELRNITSAHYADMETHISALRIDIHDLYSEYQELV 1260
Query: 1261 QDMVREIHELRLKAKTTNENCKRLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNR 1320
Q+MV+EIHELRLKAKTTNENCK LQFFKDKVPSAHKYWNIENQ+++LDQIKAKIYEAKNR
Sbjct: 1261 QEMVKEIHELRLKAKTTNENCKNLQFFKDKVPSAHKYWNIENQDNILDQIKAKIYEAKNR 1320
Query: 1321 LNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHF 1380
LN+LEDSIDRNIAGC Y+YI Q+ V EDGWSSDCSTSSSDIS+ESV+SRGK+ G
Sbjct: 1321 LNVLEDSIDRNIAGCEYRYISQHSVKEDGWSSDCSTSSSDISSESVSSRGKVLGY----- 1380
Query: 1381 NSLEGDSVICELCKKVESVKLLDSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYN 1440
MN D GTTTCLR+ELCM+Y AIHKL MQIDAF +++
Sbjct: 1381 ------------------------MNDDRGTTTCLRKELCMSYRAIHKLCMQIDAFLVHD 1440
Query: 1441 IGGDSLSEEMDQAKTTPKLRMGNAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEA 1500
IGGD LSEEMDQAKT KLRM AEA C+N SKVI ++EIKQDGGFLTRFEEAQ AIKEA
Sbjct: 1441 IGGDPLSEEMDQAKTPSKLRMEKAEAVCDNTSKVICVEEIKQDGGFLTRFEEAQEAIKEA 1500
Query: 1501 DIMLNALLRANENAKQLTGIFKQAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQV 1560
D+MLNALLRANENA QLTGI+KQAGEQLQIERDNLIEEVGQLKSSM LKD +NKLLQEQV
Sbjct: 1501 DLMLNALLRANENATQLTGIYKQAGEQLQIERDNLIEEVGQLKSSMQLKDDENKLLQEQV 1560
Query: 1561 WFNLEEVANLVSSLEGCISQTQREVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLE 1620
WF+LE+VAN +S LEGC+SQTQREVDEKFG++SCDVISFR+EMLK ISNWKSLME+ LE
Sbjct: 1561 WFSLEDVANSLSFLEGCLSQTQREVDEKFGMISCDVISFRDEMLKPISNWKSLMEEFFLE 1620
Query: 1621 IIGREFASFVLHECYVKEVCCQFARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMML 1680
I GREFASFV+H+CYV+E+C QFA FK EPSF LLKQQRCM+SN S S C+ DKDDM+L
Sbjct: 1621 ITGREFASFVVHQCYVEEMCRQFACFKGEPSFPLLKQQRCMKSNKTSGSICLTDKDDMIL 1680
Query: 1681 ISKIDRGRSELVTGLEEADAGFSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTS 1740
SKID GR+ELVT EEAD GFS DDILYENLALKKELKRKEVLLEGLLFDFRLLQEST+
Sbjct: 1681 TSKIDGGRTELVTDSEEADGGFSDDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTA 1740
Query: 1741 KTKDMKDQTEKLDYSLSQLEHELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDK 1800
KTKDMKD+TEKLD S+SQL+HELEIKASQ +NVLV QRKLE LLTDTEKAL LSNSKL+K
Sbjct: 1741 KTKDMKDETEKLDCSMSQLQHELEIKASQFDNVLVHQRKLEELLTDTEKALLLSNSKLEK 1800
Query: 1801 AKETMTIISEHNAQLKKQVEDLHLKKSEAKKQLADQQDVVNRLENEILHRTSSV--EKKL 1860
AKETM I EHN QLKKQ EDL+LKKSEA+++LA++QD+VNRLENEILH T V EKKL
Sbjct: 1801 AKETMNTIYEHNVQLKKQAEDLYLKKSEAERKLAEEQDIVNRLENEILHLTEHVISEKKL 1860
Query: 1861 LLSVEDIENELRRVISERDQLREQVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYA 1920
LLSVEDIEN+LRRVISERDQL EQVC LTDK+D+AYALADEKEAVA EA QESEASKLYA
Sbjct: 1861 LLSVEDIENKLRRVISERDQLHEQVCILTDKIDMAYALADEKEAVATEAHQESEASKLYA 1920
Query: 1921 EQKEEEVKILEHSVEELESTINMLETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVE 1980
EQKEEEVKILEHSVEELESTINMLETKV +MD EVEKN TLRESLELETQIFRKRLLAV
Sbjct: 1921 EQKEEEVKILEHSVEELESTINMLETKVQDMDAEVEKNRTLRESLELETQIFRKRLLAVG 1980
Query: 1981 NLTEVVDSGDEIVEHAQEQPRQQGSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISE 2040
NLTE VD GDE+VEHAQEQ RQQGSI+LELL+ARSRIKLLE+ERAEQDKEIKRHKEYI E
Sbjct: 1981 NLTEAVDFGDELVEHAQEQSRQQGSILLELLEARSRIKLLEDERAEQDKEIKRHKEYILE 2040
Query: 2041 LVLHADVQAMKYQQKYTNLEAMVRDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCIS 2100
LVLHAD QAMKYQQKYTNLEAMVRDASKD+SN + A +DKIDKSS RPRGSSSPFRCIS
Sbjct: 2041 LVLHADAQAMKYQQKYTNLEAMVRDASKDKSN-LMAAPLDKIDKSSARPRGSSSPFRCIS 2100
Query: 2101 NLVHQMNLEKDHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
NLVHQMNLEK+HELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK
Sbjct: 2101 NLVHQMNLEKEHELSTARLRIEELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVK 2160
Query: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKK 2220
LDLTKYANLIDQYQVQKMVVEAHLQSQQFREK QE+H LRTQI+DLNEERECYKSVLSKK
Sbjct: 2161 LDLTKYANLIDQYQVQKMVVEAHLQSQQFREKVQEVHDLRTQINDLNEERECYKSVLSKK 2220
Query: 2221 EAEALSTQIACEKLRERDHLLSVQNGILKMENKNLKRRIVELDEKANTLHQTRISPQESR 2280
EAEAL+TQIA EKLRERDHLLSVQNGILKMENK LKR+I+ELDEK +TLH+TR SPQ++
Sbjct: 2221 EAEALATQIAHEKLRERDHLLSVQNGILKMENKKLKRKIIELDEKTSTLHRTRSSPQKNH 2229
Query: 2281 HAFLTKQDDELTKRLAHSKMVLSRVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
HAFLTK DDELTKRLAHSKM+LSRVND+IA Y IPSGSSSHHRSGG GNEI
Sbjct: 2281 HAFLTK-DDELTKRLAHSKMLLSRVNDDIAHYRIPSGSSSHHRSGGPGNEI 2229
BLAST of Sgr025536 vs. ExPASy TrEMBL
Match:
A0A1S3C724 (phragmoplast orienting kinesin-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497757 PE=3 SV=1)
HSP 1 Score: 3431.0 bits (8895), Expect = 0.0e+00
Identity = 1841/2308 (79.77%), Postives = 2009/2308 (87.05%), Query Frame = 0
Query: 18 SNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQDSGFDSNFKLQSIKADW 77
S ++QPE NEN G SPS LHFPPPRTPFNIIADPAQ+ KE DSGFDSN KLQS KAD
Sbjct: 8 SKNSQPEYNENELGVSPSGLHFPPPRTPFNIIADPAQFQKEFHDSGFDSNLKLQSTKADL 67
Query: 78 SSDRRSEVSLK-NGNACTNNGTPKFSAQGRWVNSEPSSTHSTPARSSSRVSFGGAIVATG 137
SDR+SEVSLK NGNACT+NGTP+FSAQGR VNSEPSSTHSTPA+SSSRVS GGAIVATG
Sbjct: 68 FSDRKSEVSLKINGNACTSNGTPRFSAQGRRVNSEPSSTHSTPAKSSSRVSLGGAIVATG 127
Query: 138 SKAPQLADGRAGSSFRLSRRISIPNTELPVEVPHFDLAEDPSFWNDHNVQVMIRIRPLST 197
SKAPQLADGRAGSS+R SRRIS+PNTE PV+V H DL EDPSFW DHNVQVMIRIRPLST
Sbjct: 128 SKAPQLADGRAGSSYRFSRRISMPNTECPVDVSHIDLEEDPSFWKDHNVQVMIRIRPLST 187
Query: 198 TERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQENLFKVAGQPMVENCLSG 257
ERDSQGYGRCLRQE+AKTLVW+GHPETRFTFDHIAC+ ISQENLFKVAGQPMVENCLSG
Sbjct: 188 MERDSQGYGRCLRQESAKTLVWLGHPETRFTFDHIACEKISQENLFKVAGQPMVENCLSG 247
Query: 258 YNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEHLFTRIGMEEKSKRDAKL 317
YNSCMFAYGQTGSGKTYTMMGGIYE+EGKLNEDCGLTLRIFEHLFTRIGMEEKSKRD KL
Sbjct: 248 YNSCMFAYGQTGSGKTYTMMGGIYELEGKLNEDCGLTLRIFEHLFTRIGMEEKSKRDVKL 307
Query: 318 KYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLTEHNVTTVNDVVKLLLQG 377
KY CKCSFLEIYNEQI DLLEPSSTNLQLRED KKGVYVENLTEH+V+T+NDVVKLLLQG
Sbjct: 308 KYSCKCSFLEIYNEQITDLLEPSSTNLQLREDSKKGVYVENLTEHSVSTINDVVKLLLQG 367
Query: 378 AANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARLNLVDLAGSERQKSSGAE 437
AANRKMAAT+MNSESSRSHSVFTCIIESHWEKDSRTH RFARLNLVDLAGSERQKSSGAE
Sbjct: 368 AANRKMAATYMNSESSRSHSVFTCIIESHWEKDSRTHLRFARLNLVDLAGSERQKSSGAE 427
Query: 438 GDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLGGNSKTTVIA 497
GDRLKEAANINKSLST+GLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLGGNSKTTVIA
Sbjct: 428 GDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLGGNSKTTVIA 487
Query: 498 NVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQRQILHLKGQLSFLLKHS 557
NVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECAS DETALQRQILHLKGQLSFLLKHS
Sbjct: 488 NVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASGDETALQRQILHLKGQLSFLLKHS 547
Query: 558 NFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKLMEATLIGALRREEVANTAI 617
NFPRSI+S+V R EE GVS F DY LG RMQT NHKMKLMEA+LIGA RREEVANT I
Sbjct: 548 NFPRSILSSVPRLEEFGVSAPFDDYGALGNRMQTENHKMKLMEASLIGASRREEVANTTI 607
Query: 618 QKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLGGMLSADKYLLEENKALV 677
+KLEFEIEHMKRLAFQQEED QRTKMLLKFREEKIRQLEL LGGM+SAD+YLL+ENKAL
Sbjct: 608 KKLEFEIEHMKRLAFQQEEDGQRTKMLLKFREEKIRQLELFLGGMVSADQYLLDENKALA 667
Query: 678 VEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGEREALLTEVAELRNELLVAL 737
VEIKMLQAKI+R+PELTRVSLENS+L EQLQV+ NFYELGEREALLTEVAELRNELLVAL
Sbjct: 668 VEIKMLQAKIDRNPELTRVSLENSKLTEQLQVYHNFYELGEREALLTEVAELRNELLVAL 727
Query: 738 GKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDAKKYLKCSQGYASVSLSKHR 797
GKNSTISERDKYQ
Sbjct: 728 GKNSTISERDKYQ----------------------------------------------- 787
Query: 798 EQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIGQGSDNDFSVKPICSQEDLI 857
+ETMSIKSY +DDT SYI G++E EN +GQGSD++ KPI S++DL
Sbjct: 788 -----------NETMSIKSYIQDDTLSYIAGSEENFENTLGQGSDDELGAKPIFSRKDLT 847
Query: 858 DLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTKCQNKGNVVDQHEDVDNKAL 917
D MLAE++DS+N+MQ E+H CKQ K M ENFIK+ + TKCQN GN+++QHEDVDNK L
Sbjct: 848 DAKMLAESMDSDNHMQAENHGCKQFKCCMVENFIKQSDGTKCQNDGNLMNQHEDVDNKTL 907
Query: 918 QVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQVEMETANTILQLQEEVETL 977
QVKLENLT+ELEEVRLSNI YQENQ +QQNQIE VRQQVEMETA+TILQLQEEVETL
Sbjct: 908 QVKLENLTRELEEVRLSNIHYQENQ----NQQNQIEDVRQQVEMETASTILQLQEEVETL 967
Query: 978 QLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMIELTSFLLDSSRSIRDAHGQ 1037
QLELNDRLHGLAQENT LKD LSAKNEEMRMLC+DWETAM+ELTSFLLDSSRSIRDAHGQ
Sbjct: 968 QLELNDRLHGLAQENTLLKDLLSAKNEEMRMLCIDWETAMVELTSFLLDSSRSIRDAHGQ 1027
Query: 1038 IEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLEDARLMVQEMELKLDSLKEA 1097
IEGI+NLFP+VNVGISEQVQ+AI+VC+EKEETIL LHK+LEDARLMV+EMELKLDSLKEA
Sbjct: 1028 IEGIANLFPEVNVGISEQVQQAIKVCIEKEETILFLHKNLEDARLMVKEMELKLDSLKEA 1087
Query: 1098 TLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLNVKKDQLVEASLS------- 1157
TLA NES+QM+DN AA AK LS +TD+NIM EFLDKRL VK L+EA S
Sbjct: 1088 TLAFNESEQMHDNISAAGAKPLSPQMTDENIMGEFLDKRLGVKNSPLIEAEKSADAAVTA 1147
Query: 1158 -QWLSYSQELACCNSIGREMPISKLDVSFQ-SSHIIDDVMANTNALLLEELDTESDLICL 1217
+WLS QEL CCNSI R+MPISKLDVS Q SSHI D++MANTN LLLEE DT S++I L
Sbjct: 1148 VEWLSQPQELGCCNSIERQMPISKLDVSSQRSSHIFDNLMANTNELLLEESDTVSNMIWL 1207
Query: 1218 GIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDMVREIHELRLKAKTTNENCK 1277
G+ ELKNIT HYADMEMHISAL+IYI DLYSEY L+QDM REIHELRLKA+T+NE+ K
Sbjct: 1208 GLTELKNITIGHYADMEMHISALHIYIQDLYSEYQELIQDMAREIHELRLKAETSNESYK 1267
Query: 1278 RLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNILEDSIDRNIAGCGYQYIGQ 1337
LQFFKDK SA KYWNIENQNS+LDQIKAKIYEAKNRLNILEDSIDRN AGCG +Y+ Q
Sbjct: 1268 SLQFFKDKDQSAQKYWNIENQNSILDQIKAKIYEAKNRLNILEDSIDRNTAGCGERYLDQ 1327
Query: 1338 YPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSLEGDSVICELCKKVESVKLL 1397
YPV EDGWSSDCSTSSS+ISTES TSRG K L
Sbjct: 1328 YPVKEDGWSSDCSTSSSEISTESDTSRG-----------------------------KFL 1387
Query: 1398 DSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGGDSLSEEMDQAKTTPKLRMG 1457
D MNG GTT LR+EL MTYNAI KL MQID M++IGG+SLSEEMDQ KT KLRM
Sbjct: 1388 DYMNGGEGTTISLRKELYMTYNAIRKLCMQIDTVLMHDIGGNSLSEEMDQGKTLFKLRME 1447
Query: 1458 NAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIMLNALLRANENAKQLTGIFK 1517
AEAGC+N SKVISI+EIKQDGGFLTRFEEAQ AIKEADIMLNALLRAN NAKQLT IFK
Sbjct: 1448 KAEAGCSNTSKVISIEEIKQDGGFLTRFEEAQEAIKEADIMLNALLRANANAKQLTDIFK 1507
Query: 1518 QAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFNLEEVANLVSSLEGCISQTQ 1577
QAGEQLQIERDNL++EVGQLKSSMHLKDA+NKLL +QV FNLEEVAN VSSLEGCISQTQ
Sbjct: 1508 QAGEQLQIERDNLVDEVGQLKSSMHLKDAENKLLHDQVCFNLEEVANSVSSLEGCISQTQ 1567
Query: 1578 REVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIGREFASFVLHECYVKEVCCQ 1637
R+VDEKFGI+SCD+IS R+EMLKS+SNWKSL+EDI LEI+GREFASFV+H+CY KE+C Q
Sbjct: 1568 RDVDEKFGIISCDIISLRDEMLKSVSNWKSLLEDIFLEIMGREFASFVIHQCYSKEICWQ 1627
Query: 1638 F-ARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISKIDRGRSELVTGLEEADAG 1697
F A+FKA+P+F LK QRC+ES N S STC+ DK+D+MLI+KI++GR+EL+TGLEE D G
Sbjct: 1628 FAAQFKADPNFLPLKWQRCLESTNASGSTCLTDKEDIMLINKIEKGRTELITGLEEVDGG 1687
Query: 1698 FSYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTKDMKDQTEKLDYSLSQLEH 1757
FSYDDILYE LALKKEL+RKEVLLEGLLFDFRLLQESTSKTKDMKD+T DYSLSQL+H
Sbjct: 1688 FSYDDILYEKLALKKELRRKEVLLEGLLFDFRLLQESTSKTKDMKDET---DYSLSQLQH 1747
Query: 1758 ELEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMTIISEHNAQLKKQVED 1817
ELEIKA+QL++VLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMT ISEHNAQLKKQVED
Sbjct: 1748 ELEIKANQLDSVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMTSISEHNAQLKKQVED 1807
Query: 1818 LHLKKSEAKKQLADQQDVVNRLENEILHRTSSVEKKLLLSVEDIENELRRVISERDQLRE 1877
L+L+K EA+KQLA+QQDV+N+LENEILH T S+EK+ +LSVEDIEN+L RVI+ERDQL E
Sbjct: 1808 LYLQKFEAEKQLAEQQDVINKLENEILHLT-SLEKRSVLSVEDIENDLSRVINERDQLHE 1867
Query: 1878 QVCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQKEEEVKILEHSVEELESTINM 1937
QVCFLTDKLDIAYA+ADEKEAVA EARQESEASKLYAE+KEEEVKILEHSVEELESTINM
Sbjct: 1868 QVCFLTDKLDIAYAMADEKEAVATEARQESEASKLYAEEKEEEVKILEHSVEELESTINM 1927
Query: 1938 LETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLTEVVDSGDEIVEHAQEQPRQQ 1997
LETKVHEMDEEVEKN TLRESLELE +I R+RLLAVENL+E +D G EIVEHAQ+QPRQ
Sbjct: 1928 LETKVHEMDEEVEKNRTLRESLELEKKILRQRLLAVENLSEDMDCGAEIVEHAQDQPRQP 1987
Query: 1998 GSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVLHADVQAMKYQQKYTNLEAMV 2057
G+I+LELL+ +SRIKLLE+ER EQDKE+KR KEYISELVLHAD QAMKYQQKYT+LE MV
Sbjct: 1988 GNILLELLETKSRIKLLEQERVEQDKEVKRLKEYISELVLHADAQAMKYQQKYTSLEVMV 2047
Query: 2058 RDASKDQSNPITATTIDKIDKSSVRPRGSSSPFRCISNLVHQMNLEKDHELSTARLRIEE 2117
RDASKD SNP+TA +DK++K+S RPRGSSSPFRCISNLVHQMNLEK+HELSTARLRIEE
Sbjct: 2048 RDASKDHSNPMTAPALDKVEKNSARPRGSSSPFRCISNLVHQMNLEKEHELSTARLRIEE 2107
Query: 2118 LEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYANLIDQYQVQKMVVEAH 2177
LE LATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYAN IDQYQVQKMV EAH
Sbjct: 2108 LELLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYANFIDQYQVQKMVTEAH 2167
Query: 2178 LQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAEALSTQIACEKLRERDHLLSV 2237
LQSQQF+EKE+E+ LRTQI+DL+EERECYKSVLSKKEAEAL QIACEKLRERDHLLS
Sbjct: 2168 LQSQQFQEKEREVQDLRTQINDLHEERECYKSVLSKKEAEALHMQIACEKLRERDHLLSS 2219
Query: 2238 QNGILKMENKNLKRRIVELDEKANTLHQTRISPQESRHAFLTKQDDELTKRLAHSKMVLS 2297
QNGILK ENKNLK++IVELD++ NTLHQT+ S ++ RH+FLTK +DELTKRLA+SKM+LS
Sbjct: 2228 QNGILKTENKNLKKKIVELDDRGNTLHQTQSSQRDLRHSFLTK-NDELTKRLANSKMLLS 2219
Query: 2298 RVNDEIARYHIPSGSSSHHRSGGSGNEI 2315
RVNDE+ARY IP GSSSHHRSGGSG EI
Sbjct: 2288 RVNDEMARYRIPRGSSSHHRSGGSGKEI 2219
BLAST of Sgr025536 vs. TAIR 10
Match:
AT3G17360.1 (phragmoplast orienting kinesin 1 )
HSP 1 Score: 1486.1 bits (3846), Expect = 0.0e+00
Identity = 999/2312 (43.21%), Postives = 1365/2312 (59.04%), Query Frame = 0
Query: 13 LDRDSSNDAQPESNENGFGTSPSALHFPPPRTPFNIIADPAQYHKELQDSGFDSNFKLQS 72
+ R+ PES EN F S F P R P N I DP+Q K FD KL+
Sbjct: 1 MSRNVPRIEMPESEENEFA---SLSLFSPSRPPLNSIPDPSQIQKANHLPHFDLVQKLEG 60
Query: 73 IKAD----WSSDRRSEVSLKNGNACTNNGTPKFSAQGRWVNSEPSSTHSTPARSSSRVSF 132
+A +++ EV L+ +++ PK + SEP+S STP R+ +RVS
Sbjct: 61 TRAQHQRTLGPEKKFEV-LEGRAGNSSDSNPKIVNRNGKSRSEPNSAQSTPTRNGARVSL 120
Query: 133 GGAIVATGSKAPQLADGRAGSSFRLSRRISIPNTELPVE-VPHFDLAEDPSFWNDHNVQV 192
GG ATG++ Q GR R+ R +SI + E PHF+L ED SFW DHNVQV
Sbjct: 121 GGG-CATGARFLQSFGGRG----RIPRGVSIAESVSFAETTPHFELNEDHSFWKDHNVQV 180
Query: 193 MIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQENLFKVAGQ 252
+IR+RPL T ER +QGYG+CL+QE+ +TLVW+GHPE RFTFDH+A +TISQE LF+VAG
Sbjct: 181 LIRLRPLGTMERANQGYGKCLKQESPQTLVWLGHPEARFTFDHVASETISQEKLFRVAGL 240
Query: 253 PMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEHLFTRIGME 312
PMVENCLSGYNSC+FAYGQTGSGKTYTMMG I E EG L EDCG+T RIFE+LF+RI ME
Sbjct: 241 PMVENCLSGYNSCVFAYGQTGSGKTYTMMGEISEAEGSLGEDCGVTARIFEYLFSRIKME 300
Query: 313 EKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLTEHNVTTVN 372
E+ +RD LK+ CKCSFLEIYNEQI DLLEPSSTNLQLREDL KGVYVENL EHNV TV+
Sbjct: 301 EEERRDENLKFSCKCSFLEIYNEQITDLLEPSSTNLQLREDLGKGVYVENLVEHNVRTVS 360
Query: 373 DVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARLNLVDLAGS 432
DV+KLLLQGA NRK+AAT MNSESSRSHSVFTC IES WEKDS T RFARLNLVDLAGS
Sbjct: 361 DVLKLLLQGATNRKIAATRMNSESSRSHSVFTCTIESLWEKDSLTRSRFARLNLVDLAGS 420
Query: 433 ERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLG 492
ERQKSSGAEGDRLKEAANINKSLST+GLVIMSLVDLAHGKHRH+PYRDSRLTFLLQDSLG
Sbjct: 421 ERQKSSGAEGDRLKEAANINKSLSTLGLVIMSLVDLAHGKHRHVPYRDSRLTFLLQDSLG 480
Query: 493 GNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQRQILHLKG 552
GNSKT +IANVSPS CS NETLSTLKFAQRAK IQNNAKVNE AS D TALQ++I LK
Sbjct: 481 GNSKTMIIANVSPSLCSTNETLSTLKFAQRAKLIQNNAKVNEDASGDVTALQQEIRKLKV 540
Query: 553 QLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKDYDPLGGRMQTANHKMKLMEATLIGALR 612
QL+ LLK+ + ++ + EES S K R + ++K M +IGALR
Sbjct: 541 QLTSLLKNHDSCGALSDCISSLEESRYSGTCKVAGET--RQDKCHCQVKNMNDNMIGALR 600
Query: 613 REEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLGGMLSADKY 672
RE++A +A+QK E EIE + L EEDA+R K++L REEK+ ++E G L +
Sbjct: 601 REKIAESALQKSEAEIERIDCLVRDMEEDAKRIKIMLNLREEKVGEMEFCTSGSLMTKEC 660
Query: 673 LLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGEREALLTEVAE 732
L+EENK L EIK+L+ I+++PELTR +LEN++L EQLQ +Q FYE GEREALL EV
Sbjct: 661 LIEENKTLKGEIKLLRDSIDKNPELTRSALENTKLREQLQRYQKFYEHGEREALLAEVTG 720
Query: 733 LRNELLVALGKNSTISERDKYQDFHTTKGLINLENPDTRQIGGNELQTDAKKYLKCSQGY 792
LR++LL + +D+ H K NE++ K++ C
Sbjct: 721 LRDQLL------DVLEAKDESFSKHVMK--------------ENEME---KEFEDCRNMN 780
Query: 793 ASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQSYITGNDERLENEIGQGSDNDFSVK 852
+S+ Q G ++ D+ QS + + R G++
Sbjct: 781 SSLIRELDEIQAGLGRYLNFDQ-----------IQSNVVASSTR-------GAEQ----- 840
Query: 853 PICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVKHDMEENFIKKLNVTKCQNKGNVVDQ 912
AET+ + + +Q E ++ +K ++G +V
Sbjct: 841 --------------AETMPTISEIQEE----------------VAISHSKNYDRGALVKT 900
Query: 913 HEDVDNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQVEMETANTIL 972
E +D LQ KL L K+LEE R N +Y+++ QLSQQ IE VR+QVE ETA TIL
Sbjct: 901 DEGIDRSILQFKLGKLMKDLEEARTLNCKYEKDHKSQLSQQEDIEVVREQVETETARTIL 960
Query: 973 QLQEEVETLQLELNDRLHGLAQENTRLKDALSAKNEEMRMLCVDWETAMIELTSFLLDSS 1032
+LQEEV LQ E R+ L +EN +KD ++A+ E+R L DWE A +ELT+F++ S
Sbjct: 961 ELQEEVIALQSEFQRRICNLTEENQSIKDTITARESEIRALNQDWEKATLELTNFIVAGS 1020
Query: 1033 RSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEETILLLHKSLEDARLMVQEME 1092
+SI++A QIE I FP+VN I + V++A + C++KEETILLL KSLEDAR++V EM
Sbjct: 1021 KSIKNASTQIESIICSFPQVNAWIGDYVEKAAKNCIKKEETILLLQKSLEDARILVAEMN 1080
Query: 1093 LKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNIMVEFLDKRLNVKKDQLVEAS 1152
LKL+SLK AT+ALNE Q AA ++ +L D + M + +D
Sbjct: 1081 LKLNSLKGATIALNEFQL---GGNAATTEEAFNLNNDVDRMSDEVD-------------- 1140
Query: 1153 LSQWLSYSQELACCNSIGREMPISKLDVSFQSSHIIDDVMANTNALLLEELDTESDLICL 1212
L+ +F+ AN ++L E E+ L
Sbjct: 1141 ------------------------TLESNFK---------ANQYSILKTERHAEAAL--- 1200
Query: 1213 GIMELKNITSAHYADMEMHISALYIYIHDLYSEYHGLVQDMVREIHELRLKAKTTNENCK 1272
A+ ++ D ++ M+ ++ + +K T
Sbjct: 1201 ---------------------AVTKWLSDSRDQH-----QMMEKVQDQSVKEFGT----- 1260
Query: 1273 RLQFFKDKVPSAHKYWNIENQNSMLDQIKAKIYEAKNRLNILEDSIDRNIAGCGYQYIGQ 1332
L I A + E + D +++ G+
Sbjct: 1261 ------------------------LSSISASL--------SAEGNADISLSRDGHLSDAT 1320
Query: 1333 YPVNEDGWSSDCSTSSSDISTESVTSRGKLNGLLCSHFNSLEGDSVICELCKKVESVKLL 1392
YP + + STSSSD S LN + C +S E D+ E K+ S L+
Sbjct: 1321 YPKGD-----ELSTSSSDFSNCRWQHDCALN-VKCQGVSSSESDAQ--ESNNKITSAALI 1380
Query: 1393 DSMNGDSGTTTCLRRELCMTYNAIHKLSMQIDAFQMYNIGGDSLSEEMDQAKTTPKLRMG 1452
+ NG + + C + S++ M + E + K + L G
Sbjct: 1381 -AKNGSAHSVYC----------GEGRQSVEKPLTIM-------MGREETEYKCSKPLSSG 1440
Query: 1453 NAEAGCNNISKVISIKEIKQDGGFLTRFEEAQAAIKEADIMLNALLRANENAKQLTGIFK 1512
+ V + F RFEE A +KEAD+ + L++ANE + +T ++
Sbjct: 1441 VYMGLMQRMDPVRT---------FFDRFEEVNATMKEADLTICELVKANEKSNSVTEMWL 1500
Query: 1513 QAGEQLQIERDNLIEEVGQLKSSMHLKDADNKLLQEQVWFNLEEVANLVSSLEGCISQTQ 1572
Q E+L + NL++++ Q+KS + + + ++L Q L ++ N VS LE + +
Sbjct: 1501 QTHEELISKEKNLMDDLEQVKSILSACEEEKQVLLNQTHTTLADMENSVSLLEEYFQEMK 1560
Query: 1573 REVDEKFGIMSCDVISFRNEMLKSISNWKSLMEDICLEIIGREFASFVLHECYVKEVCCQ 1632
R V+E + E+L+ ISN + +E I E + REF + ++C++ ++ Q
Sbjct: 1561 RGVEETVEALFSHARLAGKELLQLISNSRPSLEQIASEFMEREFTMYATYQCHIGKLIDQ 1620
Query: 1633 FARFKAEPSFQLLKQQRCMESNNNSRSTCIADKDDMMLISKIDRGRSELVTGLEEADAGF 1692
+ + L Q E+N + + I + + K + R E+VTGLE +
Sbjct: 1621 ILDQRKQVITPNLSGQ---ETNQSVKINAIGYNAEDEVTKK--QSREEIVTGLENDEVVQ 1680
Query: 1693 SYDDILYENLALKKELKRKEVLLEGLLFDFRLLQESTSKTKDMKDQTEKLDYSLSQLEHE 1752
S++ +LYENL LKKEL+RKE L EGLLFDFRLLQES S +D+K++ ++L +L +++ E
Sbjct: 1681 SHESLLYENLYLKKELERKEALFEGLLFDFRLLQESASNKRDIKNEMDELFDALCKVQLE 1740
Query: 1753 LEIKASQLENVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMTIISEHNAQLKKQVEDL 1812
LE+KASQ+ + V LE D + ALF S S L++AK+ + I++E N +L+ V DL
Sbjct: 1741 LELKASQVHELFVHNENLENCSIDLKTALFTSQSDLEQAKQRIQILAEQNDELRALVSDL 1800
Query: 1813 HLKKSEAKKQLADQQDVVNRLENEILHRTSSVEKKLLLSVEDIENELRRVISERDQLREQ 1872
+K+ A++ L +Q+D+VNRLE EILH T++ EK+LL +V+ I+ L++ E+DQ+ ++
Sbjct: 1801 CKEKAAAEEGLDEQRDLVNRLEKEILHLTTTAEKQLLSAVKSIKENLKKTSDEKDQIVDE 1860
Query: 1873 VCFLTDKLDIAYALADEKEAVAIEARQESEASKLYAEQKEEEVKILEHSVEELESTINML 1932
+C L +KL++AYA+ADEKEA+A+EA QESEASK+YAEQKEEEVKILE SVEELE TIN+L
Sbjct: 1861 ICSLNNKLELAYAIADEKEAIAVEAHQESEASKIYAEQKEEEVKILEISVEELERTINIL 1920
Query: 1933 ETKVHEMDEEVEKNCTLRESLELETQIFRKRLLAVENLT-EVVDSGDEIVEHAQEQPRQQ 1992
E +V++MDEEV+++ T ++SLE E Q R+RL EN T +V + + E+ R
Sbjct: 1921 ERRVYDMDEEVKRHRTTQDSLETELQALRQRLFRFENFTGTMVTTNESTEEYKSHISRST 1980
Query: 1993 GSIILELLDARSRIKLLEEERAEQDKEIKRHKEYISELVLHADVQAMKYQQKYTNLEAMV 2052
G L A S+I++L++E AEQ KEIK+ KEYISE++LH++ Q+ YQ+KY LE M+
Sbjct: 1981 G-----LQGAHSQIQVLQKEVAEQTKEIKQLKEYISEILLHSEAQSSAYQEKYKTLEVMI 2040
Query: 2053 RDASKDQSNPITATTID-KIDKSSVRPRGSSSPFRCISNLVHQMNLEKDHELSTARLRIE 2112
RD + S+ A TI K +KSS R RGSSSPFRCI LV QM LEKD EL+ AR+R+E
Sbjct: 2041 RDFKLEDSSSSAAETISHKTEKSSTRSRGSSSPFRCIVGLVQQMKLEKDQELTMARVRVE 2061
Query: 2113 ELEGLATSRQKEICILNARLAAAESMTHDVIRDLLGVKLDLTKYANLIDQYQVQKMVVEA 2172
ELE L +QKEIC LN R+AAA+SMTHDVIRDLLGVK+D+T YA LIDQ+QVQ++V +A
Sbjct: 2101 ELESLLAVKQKEICTLNTRIAAADSMTHDVIRDLLGVKMDITSYAELIDQHQVQRVVEKA 2061
Query: 2173 HLQSQQFREKEQEIHYLRTQISDLNEERECYKSVLSKKEAEALSTQIACEKLRERDHLLS 2232
+++ KEQE+ L+ I L ++RE S L+KK+ + L+TQI+ ++L+ER LLS
Sbjct: 2161 QQHAEEILSKEQEVMNLKRHIDYLFKDRESCMSELNKKDTDVLATQISLDQLQERVQLLS 2061
Query: 2233 VQNGILKMENKNLKRRIVELDEKANTLHQTRIS----PQESRH-AFLTKQDDELTKRLAH 2292
+QN +LK + NL R++ ELD T+H + S PQ ++ A D + TKRL +
Sbjct: 2221 MQNEMLKNDKSNLLRKLAELD---RTVHNAQASNHRVPQTTKDTASFKLADTDYTKRLEN 2061
Query: 2293 SKMVLSRVNDEIARYHIPSGSSSHHRSGGSGN 2313
++ +LS N+E+A+Y S + R+ G +
Sbjct: 2281 AQKLLSHANNELAKYRKTSNNHPSTRTQGQSS 2061
BLAST of Sgr025536 vs. TAIR 10
Match:
AT3G19050.1 (phragmoplast orienting kinesin 2 )
HSP 1 Score: 979.2 bits (2530), Expect = 5.9e-285
Identity = 856/2597 (32.96%), Postives = 1279/2597 (49.25%), Query Frame = 0
Query: 91 NACTNNGTPKFSAQGRWVNSEPSSTHSTPARSSSR---VSFGGAIVATGSKAPQLADGRA 150
NA N TPK SE +ST +TP +S S+ + G + TG+ RA
Sbjct: 99 NAALLNTTPKTGRVVGRAYSETNSTQNTPTKSVSKPPGSCYRGKLDGTGTV-------RA 158
Query: 151 GSSFRLSRRISIPNTEL-----PVEVPHFDLAEDPSFWNDHNVQVMIRIRPLSTTERDSQ 210
G L + +S + ++ VEVPHF L EDPSFW DHNVQ++IR+RPL++ ER
Sbjct: 159 GGYASLYKGLSSSSGQVSTVVNSVEVPHFSLKEDPSFWMDHNVQILIRVRPLNSMERSIN 218
Query: 211 GYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQENLFKVAGQPMVENCLSGYNSCMF 270
GY RCL+QE+++ + WIG PETRF FDH+AC+TI QE LF+VAG PMVENCLSGYNSC+F
Sbjct: 219 GYNRCLKQESSQCVAWIGPPETRFQFDHVACETIDQETLFRVAGLPMVENCLSGYNSCIF 278
Query: 271 AYGQTGSGKTYTMMGGIYEVEGKLNEDCGLTLRIFEHLFTRIGMEEKSKRDAKLKYCCKC 330
AYGQTGSGKTYTM+G + ++E K + + G+ RIFE LF RI EE+S+RD +LKY CKC
Sbjct: 279 AYGQTGSGKTYTMLGEVGDLEFKPSPNRGMMPRIFEFLFARIQAEEESRRDERLKYNCKC 338
Query: 331 SFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVENLTEHNVTTVNDVVKLLLQGAANRKM 390
SFLEIYNEQI DLLEPSSTNLQLRED+K GVYVENLTE V +V D++ L+ QG+ NR++
Sbjct: 339 SFLEIYNEQITDLLEPSSTNLQLREDIKSGVYVENLTECEVQSVQDILGLITQGSLNRRV 398
Query: 391 AATHMNSESSRSHSVFTCIIESHWEKDSRTHFRFARLNLVDLAGSERQKSSGAEGDRLKE 450
AT+MN ESSRSHSVFTC+IES WEKDS + RFARLNLVDLAGSERQK+SGAEGDRLKE
Sbjct: 399 GATNMNRESSRSHSVFTCVIESRWEKDSTANMRFARLNLVDLAGSERQKTSGAEGDRLKE 458
Query: 451 AANINKSLSTIGLVIMSLVDLAHGKHRHIPYRDSRLTFLLQDSLGGNSKTTVIANVSPSF 510
AA+INKSLST+G VIM LVD+A+GK RHIPYRDSRLTFLLQDSLGGNSKT +IAN SPS
Sbjct: 459 AASINKSLSTLGHVIMVLVDVANGKPRHIPYRDSRLTFLLQDSLGGNSKTMIIANASPSV 518
Query: 511 CSANETLSTLKFAQRAKQIQNNAKVNECASSDETALQRQILHLKGQLSFLLKHSNFPRSI 570
A ETL+TLKFAQRAK IQNNA VNE ++ D L+RQI LK +LS LLK N R++
Sbjct: 519 SCAAETLNTLKFAQRAKLIQNNAVVNEDSNEDVLELRRQIRLLKEELS-LLKRQNISRAL 578
Query: 571 M--SNVQRFEESGV---SDLFKD-----------YDPLGGRMQTANHKMKLMEATLIGAL 630
S F ES V S + + Y+ GG ++ + ++K +E TL G+L
Sbjct: 579 SFGSATANFAESQVDSPSSVMHETGQQQAGNLLVYES-GGCVRMSRKQLKSLEITLAGSL 638
Query: 631 RREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLELLLGGMLSADK 690
RRE VA+ +I+KLE EIEH+ RL Q+EED + TKM+L+FRE+KI++LE LLG +SAD
Sbjct: 639 RREHVADASIKKLEAEIEHLNRLVRQREEDTRSTKMMLRFREDKIQRLESLLGNHISADS 698
Query: 691 YLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFYELGEREALLTEVA 750
+LLEEN L EI++LQAKI+++PELTR +LEN L++QL+ FQ FYE GERE LL EV+
Sbjct: 699 FLLEENNVLSEEIQLLQAKIDKNPELTRFALENIRLLDQLRRFQEFYEEGEREILLGEVS 758
Query: 751 ELRNELLVALGKNS-----------------------TISERDKYQDFHTTKGLINL--- 810
LRN+L L +NS ++ E K + K NL
Sbjct: 759 NLRNQLFQFLDENSDWQKHVDDGIEPQGASRMSKENCSLQEELKKTCYELEKCRSNLGSC 818
Query: 811 --ENPD-TRQIGGNELQTDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMS----- 870
EN +R+I N+LQ C+ S S++K + G ++ ET++
Sbjct: 819 LEENAKLSREI--NDLQAMVSDIRACTPDEHS-SVNKQKALLGTQN-FEPHETLACEQAN 878
Query: 871 -----IKSYSRDDTQSYITGNDERLENEI-GQGSDNDFSVKPICSQEDLID------LGM 930
IK D Q I + L + Q F ++ + Q LI
Sbjct: 879 YVEEIIKLQLDLDVQKIILDEERTLRGDTEAQAVRLKFDIEVLKDQLLLISKQQKNVYSE 938
Query: 931 LAET------VDSEN-NMQGEDHRCKQVKHDMEENFIKKLNVTKCQNKGNVVDQHED--V 990
L ET ++S+N + E +++K + E +KK + K D+ +D
Sbjct: 939 LGETKSAVAALESQNIILIQEAVELRRIKENYFE-LLKKQELDIPAMKSKQCDEFKDNPA 998
Query: 991 DNKALQVKLENLTKELEEVRLSNIQYQENQIFQLSQQNQIEHVRQQVEMETANTILQLQE 1050
++ + K + + LE+ + N+ Y+ + + +++ V +Q E TA I+ LQ
Sbjct: 999 EDSEIDTKFKKMQASLEKAKRLNMLYKSDIASKACGDEEMDEVCKQAEAATAEVIVCLQN 1058
Query: 1051 EVETLQLELN------------------------DRLHGLAQENTRLKDALSAKNEEMRM 1110
E+E LQ E+N D+L +N +L++ L K+ E+ +
Sbjct: 1059 ELEVLQKEVNDFQSKENVTEKQVEILETQMEELQDKLRDTTMDNEQLQEQLRGKDMELLI 1118
Query: 1111 LCVDWETAMIELTSFLLDSSRSIRDAHGQIEGISNLFPKVNVGISEQVQRAIEVCVEKEE 1170
+ + E EL LL+ + + DA Q + IS P + ISEQV I E+E
Sbjct: 1119 ISNEMELLTSELEEILLNGNEGLTDACYQADLISGSLPDKRIWISEQVGGLIRTLSEREL 1178
Query: 1171 TILLLHKSLEDARLMVQEMELKLDSLKEATLALNESQQMYDNKCAADAKQLSHLLTDKNI 1230
I L LEDA ++E L SLK A + +NE+ Q + D L L K
Sbjct: 1179 MIEDLESCLEDANKKRCDIESMLKSLKGAAIVMNEAHQREFEEKETDVLLLKSQLCTKTE 1238
Query: 1231 MVEFLDKRLNVKKDQLVEASLSQWLS------YSQ------------ELACCNSIGR--- 1290
+ L ++L + + + EAS S YS+ + S G
Sbjct: 1239 TILRLQEKLKMAERLIYEASDCATASLIIVNRYSEVTESHTFELKQKDFQVAESTGTILS 1298
Query: 1291 -EMPISKLDVS---FQSSHIIDDVMANTNALLLEEL-DTESDLICLGIMELKNITSAHYA 1350
+ + L+ + F+S + ++ A+ LEE+ +T + + ELK S
Sbjct: 1299 LKQQVQDLEATCKEFRSKLLEEEKNASAMEQKLEEIEETSISAMKEKLSELKGGVS---- 1358
Query: 1351 DMEMHISALYIYIHDLYSEYHGLV---------QDMVREI---------------HELRL 1410
++ HD Y+E + Q+ R + +RL
Sbjct: 1359 --DLRSCITMCQEHDKYTEAENSLSSPAHCSEGQEPGRNVVVSSCIEKTPNNNHTESMRL 1418
Query: 1411 KAKTTNENCKRLQFFKDKVPSA---HKYWNIENQN----------------SMLDQIKAK 1470
+K ++E K + K ++ SA K +E N S L+ + A+
Sbjct: 1419 SSKVSSERGKVIILLKQEMESALASLKEVQVEMANLKGEKEELKASEKRSLSNLNDLAAQ 1478
Query: 1471 I-------------YEAK-----NRLNILEDSIDRNIAGCGYQYIGQYPVNEDGWSSDCS 1530
I YE K ++L LE I + +Y+ + + + +
Sbjct: 1479 ICNLNTVMSNMEEQYEHKMEVTDHKLKTLEHEIAKMKIEADQEYVENLCILKKFEEAQGT 1538
Query: 1531 TSSSDIS-TESVTSRGKLNGLLCSHFNSLEGDSVICELCKKVESVKLLDSMNGDSGTTTC 1590
+DI+ E V + K+ L G S++ E VE ++ L+S+N
Sbjct: 1539 IREADITVNELVIANEKMRFDL--EKQKKRGISLVGEKKALVEKLQELESINVKENEKLA 1598
Query: 1591 LRRELCMTY-----NAIHKLSMQIDAFQ-MYNIGGDSLSEEMDQAKT----TPKLRMGNA 1650
+L + N + +L+ + Q ++ +++++ + K+ T R+
Sbjct: 1599 YLEKLFESSLMGIGNLVEELATVVRKLQDESSVALTGMAKDLSELKSWVSETNSARLFLE 1658
Query: 1651 EAGCNNISK---------------VISIKEIKQDGGFLTR----FEEAQAAIKEADIMLN 1710
+ I K + ++ I + G L R + A +++ ++ L
Sbjct: 1659 DIWSEIIMKDCAISVLHLCHMGILLETVTGINTENGLLQRGLCVSNSSIAGLRDNNLRLR 1718
Query: 1711 ALLR--ANENAKQLTGI-----------------------FKQAGEQLQIERDNLIEEVG 1770
L AN K LT I F Q LQ + D +++
Sbjct: 1719 RELEMFANLKGKLLTDIKNGFERISRNEEATNLLTTKLSSFDQKISGLQYQEDLMLQRSN 1778
Query: 1771 QLKSSMH--LKDAD-------NKLLQEQVWFN-----------------------LEEVA 1830
+ S + LK+ D LL+++ N L +A
Sbjct: 1779 SMGSQLDILLKEIDLSNGDLAETLLEQERHLNQKNDFFDTEVQLYLMDLCSKDVELLVLA 1838
Query: 1831 NLVSSLEGCISQTQREVDEKFGIM------------------------SCDVISFRNEML 1890
C++ RE+ + I+ + +S + E+
Sbjct: 1839 QTAKEYSSCLAVVDRELLDHHVIVEDLKEKLIVSQVEGELKDQCLVDNKLETVSVKEELT 1898
Query: 1891 KSISNWKSLMEDIC--------LEIIGREFASFVL------------------------- 1950
++ S K L D+ ++ + ++F V+
Sbjct: 1899 EAQSKIKVLSSDLDRSVQKIAEIDEVNKDFGERVIFLESSITGLQQELAMKASELYSLEH 1958
Query: 1951 ------HECYVKEVCCQ-------------------FARFKAEPSFQLLKQQR-----CM 2010
E +KE Q F F E F+ L R C
Sbjct: 1959 SRSVTAEELDIKERDVQVYADIVSSLKKENVSLKNKFIHF-GEDQFKALDVTRLSIAKCS 2018
Query: 2011 ESNNNSR--------STCIADKDDMMLISKIDRGR--SELVTGLEEADAGFSYDDILYEN 2070
+S+ I+DK ++ +D+ ++ V L+ ++L EN
Sbjct: 2019 HLTEDSKKLEKLTRDGMAISDKMLQLICENVDKASVFADTVQSLQ-----IDVQELLSEN 2078
Query: 2071 LALKKELKRKEVLLEGLLFDFRLLQESTSKTKDMKDQTEKLDYSLSQLEHELEIKASQLE 2130
L L EL RK+ +L+GL FD LLQES S ++D KD+T+++ + LE L +K +LE
Sbjct: 2079 LNLHDELLRKDDVLKGLSFDLSLLQESASNSRDKKDETKEIMVHVEALEKTLALKTFELE 2138
Query: 2131 NVLVQQRKLEGLLTDTEKALFLSNSKLDKAKETMTIISEHNAQLKKQVEDLHLKKSEAKK 2190
+ + + LE L ++++ +KA++ +S N ++ + EDL +K ++
Sbjct: 2139 DAVSHAQMLEVRLQESKEITRNLEVDTEKARKCQEKLSAENKDIRAEAEDLLAEKCSLEE 2198
Query: 2191 QLADQQDVVNRLENEILHRTSSVEKKLLLSVEDIENELRRVISERDQLREQVCFLTDKLD 2250
++ + V +E E+ + +++ +L +V + +L I ERD L+++V L ++
Sbjct: 2199 EMIQTKKVSESMEMELFNLRNAL-GQLNDTVAFTQRKLNDAIDERDNLQDEVLNLKEEFG 2258
Query: 2251 IAYALADEKEAVAIEARQESEASKLYAEQKEEEVKILEHSVEELESTINMLETKVHEMDE 2305
+ A E EA IEA+Q +E+ K YA+++EEEVK+LE SVEELE TIN+LE KV+ + +
Sbjct: 2259 KMKSEAKEMEARYIEAQQIAESRKTYADEREEEVKLLEGSVEELEYTINVLENKVNVVKD 2318
BLAST of Sgr025536 vs. TAIR 10
Match:
AT3G44050.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )
HSP 1 Score: 551.2 bits (1419), Expect = 3.9e-156
Identity = 370/971 (38.11%), Postives = 560/971 (57.67%), Query Frame = 0
Query: 171 FDLAEDPSFWNDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDH 230
F+ EDP+FW DHNVQV+IR RPLS++E QG +C+RQ+ + + WIG+PE+RFTFD
Sbjct: 80 FEFNEDPAFWKDHNVQVIIRTRPLSSSEISVQGNNKCVRQDNGQAITWIGNPESRFTFDL 139
Query: 231 IACDTISQENLFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMGGIYEVEGKLNEDC 290
+A + +SQE +FKVAG PMVEN ++GYNSCMFAYGQTGSGKT+TM+G I + + +C
Sbjct: 140 VADENVSQEQMFKVAGVPMVENVVAGYNSCMFAYGQTGSGKTHTMLGDIEGGTRRHSVNC 199
Query: 291 GLTLRIFEHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLK 350
G+T R+FE+LF+RI E++ +++ KL + C+CSFLEIYNEQI DLL+PSS NLQLRED K
Sbjct: 200 GMTPRVFEYLFSRIQKEKEVRKEEKLHFTCRCSFLEIYNEQILDLLDPSSYNLQLREDHK 259
Query: 351 KGVYVENLTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEKDS 410
KG++VENL E V++ DV++ L+QGAANRK+AAT+MN SSRSHSVFTCIIES W
Sbjct: 260 KGIHVENLKEIEVSSARDVIQQLMQGAANRKVAATNMNRASSRSHSVFTCIIESKWVSQG 319
Query: 411 RTHFRFARLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAHGKHRH 470
TH RFARLNLVDLAGSERQKSSGAEG+RLKEA NINKSLST+GLVIM+LV +++GK H
Sbjct: 320 VTHHRFARLNLVDLAGSERQKSSGAEGERLKEATNINKSLSTLGLVIMNLVSVSNGKSVH 379
Query: 471 IPYRDSRLTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNEC 530
+PYRDS+LTFLLQDSLGGNSKT +IAN+SPS + ETLSTLKFAQRAK I+NNA VNE
Sbjct: 380 VPYRDSKLTFLLQDSLGGNSKTIIIANISPSSSCSLETLSTLKFAQRAKLIKNNAIVNED 439
Query: 531 ASSDETALQRQILHLKGQLSFLLKHSNFPRSIMSNVQRFEESGVSDLFKD-----YDPLG 590
AS D A++ QI LK +++ L M + + L D + PL
Sbjct: 440 ASGDVIAMRLQIQQLKKEVTRLRGMGGVDNQDMDTISMGCPASPMSLKWDGFNGSFTPLT 499
Query: 591 GRMQTANHKMKLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLK 650
+ + K+K E L+GA RRE + A+Q L E E +L ++E++ + KM+LK
Sbjct: 500 THKRMS--KVKDYEVALVGAFRREREKDVALQALTAENEASMKLEKKREDEIRGLKMMLK 559
Query: 651 FREEKIRQLELLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQ 710
R+ I+ L+ + G + + +L +E L+ EI++L+A+++R+ E+T+ + EN L E+
Sbjct: 560 LRDSAIKSLQGVTSGKIPVEAHLQKEKGDLMKEIEVLRAQVDRNQEVTKFATENLRLKEE 619
Query: 711 LQVFQNFYELGEREALLTEVAELRNELLVALGKNSTISERDK---YQDFHTTKGLINLEN 770
++ ++ E GER+ L ++ L+ +LL AL + E D +D + + + +N
Sbjct: 620 IRRLKSQGEEGERDILNQQIQALQAKLLEALDW-KLMHESDSSMVKEDGNISNMFCSNQN 679
Query: 771 PDTRQIGGNELQTDAKKYLKCSQGYASVSLSKHREQFGRRDGISMDETMSIKSYSRDDTQ 830
+++++ + D ++L+ ++ E + S+DE ++ Q
Sbjct: 680 QESKKLSSIQ---DENEFLRMQ----AIQNRAEMESLQKSLSFSLDE--------KERLQ 739
Query: 831 SYITGNDERLENEIGQGSDNDFSVKPICSQEDLIDLGMLAETVDSENNMQGEDHRCKQVK 890
+ LE +I + +D +++ + + + + + E H +K
Sbjct: 740 KLVDNLSNELEGKIRSSG--------MVGDDDQMEVKTMVQAIACVSQREAEAHE-TAIK 799
Query: 891 HDMEENFIKKLNVTKCQNKGNVVDQHEDVDNKALQVKLENLTKELEEVRLSNIQYQENQI 950
E + +++ ++ +++ +E QV EN ++ ++ ++
Sbjct: 800 LSKENDDLRQKIKVLIEDNNKLIELYE-------QVAEENSSRAWGKIE------TDSSS 859
Query: 951 FQLSQQNQIEHVRQQVEMETANTILQLQEEVETLQLELNDRLHGLAQENTRL----KDAL 1010
QN E + +VE A EE + + L ++L + EN +L ++A+
Sbjct: 860 NNADAQNSAE-IALEVEKSAA-------EEQKKMIGNLENQLTEMHDENEKLMSLYENAM 919
Query: 1011 SAKNEEMRMLCVDWETAMIELTSFL------LDSSRSIRDAHGQIEGISNLFPKVNVGIS 1070
K+E R+L + IE S + S +S D + + K+++
Sbjct: 920 KEKDELKRLLSSPDQKKPIEANSDTEMELCNISSEKSTEDLNSAKLKLELAQEKLSIS-- 979
Query: 1071 EQVQRAIEVCVEKEETIL---LLHKSLEDARLMVQEMELKLDSLKEATLALNESQQMYDN 1121
+ I V EE IL L K ++ V+E + +L S+K + N +++ +
Sbjct: 980 ---AKTIGVFSSLEENILDIIKLSKESKETEEKVKEHQSELGSIKTVSDQTNARKEVAEK 997
BLAST of Sgr025536 vs. TAIR 10
Match:
AT3G23670.1 (phragmoplast-associated kinesin-related protein, putative )
HSP 1 Score: 361.7 bits (927), Expect = 4.4e-99
Identity = 198/375 (52.80%), Postives = 260/375 (69.33%), Query Frame = 0
Query: 181 NDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQEN 240
+D V+V++R++P S E + + ++ + + I E FTFD IA +Q+
Sbjct: 93 SDSGVKVIVRMKPPSKGEEEEM-----IVKKISNDALTIN--EQTFTFDSIADPESTQDE 152
Query: 241 LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMG---GIYEVEGKLNEDCGLTLRIF 300
+F++ G P+VENCL+G+NS +FAYGQTGSGKTYTM G G+ E E + GLT R+F
Sbjct: 153 IFQLVGAPLVENCLAGFNSSVFAYGQTGSGKTYTMWGPANGLLE-EHLSGDQRGLTPRVF 212
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
E LF R+ E+ + +LKY C+CSFLEIYNEQI DLL+PS NL +RED+K GVYVEN
Sbjct: 213 ELLFARLSEEQAKHAERQLKYQCRCSFLEIYNEQITDLLDPSLKNLMIREDVKSGVYVEN 272
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEK--DSRTHFR 420
LTE V + D+ KLL++G ANR+ AT +N+ESSRSH VFTC++ESH + D + F+
Sbjct: 273 LTEEYVKNLKDLSKLLVKGLANRRTGATSVNAESSRSHCVFTCVVESHCKSVADGLSSFK 332
Query: 421 FARLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAH-GKHRHIPYR 480
+R+NLVDLAGSERQK +GA GDRLKEA NIN+SLS +G +I L +++ GK RHIPYR
Sbjct: 333 TSRINLVDLAGSERQKLTGAAGDRLKEAGNINRSLSQLGNLINILAEISQTGKQRHIPYR 392
Query: 481 DSRLTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSD 540
DSRLTFLLQ+SLGGN+K ++ VSPS +ET STL+FAQRAK IQN A VNE D
Sbjct: 393 DSRLTFLLQESLGGNAKLAMVCAVSPSQSCRSETFSTLRFAQRAKAIQNKAIVNEVMQDD 452
Query: 541 ETALQRQILHLKGQL 550
L+ I L+ +L
Sbjct: 453 VNFLREVIRQLRDEL 459
HSP 2 Score: 75.1 bits (183), Expect = 8.3e-13
Identity = 51/151 (33.77%), Postives = 85/151 (56.29%), Query Frame = 0
Query: 596 KLMEATLIGALRREEVANTAIQKLEFEIEHMKRLAFQQEEDAQRTKMLLKFREEKIRQLE 655
K +E L G++RRE K EI + RL Q + + + ++ + RE+KI +LE
Sbjct: 860 KAVEKVLAGSIRREMAMEEFCTKQASEISQLNRLVQQYKHERECNAIIGQTREDKIVRLE 919
Query: 656 LLLGGMLSADKYLLEENKALVVEIKMLQAKINRSPELTRVSLENSELIEQLQVFQNFY-E 715
L+ G+LS D +L EE +L+ E K+L+ PE+ + +E + E+L+ F+NFY +
Sbjct: 920 SLMDGVLSKDDFLDEEFASLMHEHKLLKDMYENHPEVLQTRIELKRVQEELESFKNFYGD 979
Query: 716 LGEREALLTEVAELRNELLVALGKNSTISER 746
+GERE LL E+ +L+ +L + T + R
Sbjct: 980 MGEREVLLEEIHDLKAQLQCYTDSSLTSARR 1010
BLAST of Sgr025536 vs. TAIR 10
Match:
AT3G23670.2 (phragmoplast-associated kinesin-related protein, putative )
HSP 1 Score: 361.7 bits (927), Expect = 4.4e-99
Identity = 198/375 (52.80%), Postives = 260/375 (69.33%), Query Frame = 0
Query: 181 NDHNVQVMIRIRPLSTTERDSQGYGRCLRQETAKTLVWIGHPETRFTFDHIACDTISQEN 240
+D V+V++R++P S E + + ++ + + I E FTFD IA +Q+
Sbjct: 93 SDSGVKVIVRMKPPSKGEEEEM-----IVKKISNDALTIN--EQTFTFDSIADPESTQDE 152
Query: 241 LFKVAGQPMVENCLSGYNSCMFAYGQTGSGKTYTMMG---GIYEVEGKLNEDCGLTLRIF 300
+F++ G P+VENCL+G+NS +FAYGQTGSGKTYTM G G+ E E + GLT R+F
Sbjct: 153 IFQLVGAPLVENCLAGFNSSVFAYGQTGSGKTYTMWGPANGLLE-EHLSGDQRGLTPRVF 212
Query: 301 EHLFTRIGMEEKSKRDAKLKYCCKCSFLEIYNEQIADLLEPSSTNLQLREDLKKGVYVEN 360
E LF R+ E+ + +LKY C+CSFLEIYNEQI DLL+PS NL +RED+K GVYVEN
Sbjct: 213 ELLFARLSEEQAKHAERQLKYQCRCSFLEIYNEQITDLLDPSLKNLMIREDVKSGVYVEN 272
Query: 361 LTEHNVTTVNDVVKLLLQGAANRKMAATHMNSESSRSHSVFTCIIESHWEK--DSRTHFR 420
LTE V + D+ KLL++G ANR+ AT +N+ESSRSH VFTC++ESH + D + F+
Sbjct: 273 LTEEYVKNLKDLSKLLVKGLANRRTGATSVNAESSRSHCVFTCVVESHCKSVADGLSSFK 332
Query: 421 FARLNLVDLAGSERQKSSGAEGDRLKEAANINKSLSTIGLVIMSLVDLAH-GKHRHIPYR 480
+R+NLVDLAGSERQK +GA GDRLKEA NIN+SLS +G +I L +++ GK RHIPYR
Sbjct: 333 TSRINLVDLAGSERQKLTGAAGDRLKEAGNINRSLSQLGNLINILAEISQTGKQRHIPYR 392
Query: 481 DSRLTFLLQDSLGGNSKTTVIANVSPSFCSANETLSTLKFAQRAKQIQNNAKVNECASSD 540
DSRLTFLLQ+SLGGN+K ++ VSPS +ET STL+FAQRAK IQN A VNE D
Sbjct: 393 DSRLTFLLQESLGGNAKLAMVCAVSPSQSCRSETFSTLRFAQRAKAIQNKAIVNEVMQDD 452
Query: 541 ETALQRQILHLKGQL 550
L+ I L+ +L
Sbjct: 453 VNFLREVIRQLRDEL 459
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022138447.1 | 0.0e+00 | 82.31 | kinesin-like protein KIN-12C isoform X3 [Momordica charantia] | [more] |
XP_022138448.1 | 0.0e+00 | 82.30 | kinesin-like protein KIN-12C isoform X4 [Momordica charantia] | [more] |
XP_022138445.1 | 0.0e+00 | 82.20 | kinesin-like protein KIN-12C isoform X1 [Momordica charantia] | [more] |
XP_022138446.1 | 0.0e+00 | 82.20 | kinesin-like protein KIN-12C isoform X2 [Momordica charantia] | [more] |
QWT43323.1 | 0.0e+00 | 80.85 | kinesin-like protein KIN12B [Citrullus lanatus subsp. vulgaris] | [more] |
Match Name | E-value | Identity | Description | |
Q27IK7 | 0.0e+00 | 43.21 | Kinesin-like protein KIN-12C OS=Arabidopsis thaliana OX=3702 GN=KIN12C PE=1 SV=1 | [more] |
B9FUF9 | 0.0e+00 | 36.09 | Kinesin-like protein KIN-12E OS=Oryza sativa subsp. japonica OX=39947 GN=KIN12E ... | [more] |
B9GE13 | 2.7e-303 | 33.36 | Kinesin-like protein KIN-12F OS=Oryza sativa subsp. japonica OX=39947 GN=KIN12F ... | [more] |
Q27IK6 | 8.2e-284 | 32.96 | Kinesin-like protein KIN-12D OS=Arabidopsis thaliana OX=3702 GN=KIN12D PE=2 SV=1 | [more] |
Q75LL2 | 1.8e-166 | 53.94 | Kinesin-like protein KIN-12G OS=Oryza sativa subsp. japonica OX=39947 GN=KIN12G ... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1CB46 | 0.0e+00 | 82.31 | kinesin-like protein KIN-12C isoform X3 OS=Momordica charantia OX=3673 GN=LOC111... | [more] |
A0A6J1CD04 | 0.0e+00 | 82.30 | kinesin-like protein KIN-12C isoform X4 OS=Momordica charantia OX=3673 GN=LOC111... | [more] |
A0A6J1C9G8 | 0.0e+00 | 82.20 | kinesin-like protein KIN-12C isoform X1 OS=Momordica charantia OX=3673 GN=LOC111... | [more] |
A0A6J1C9Q8 | 0.0e+00 | 82.20 | kinesin-like protein KIN-12C isoform X2 OS=Momordica charantia OX=3673 GN=LOC111... | [more] |
A0A1S3C724 | 0.0e+00 | 79.77 | phragmoplast orienting kinesin-1 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103497... | [more] |
Match Name | E-value | Identity | Description | |
AT3G17360.1 | 0.0e+00 | 43.21 | phragmoplast orienting kinesin 1 | [more] |
AT3G19050.1 | 5.9e-285 | 32.96 | phragmoplast orienting kinesin 2 | [more] |
AT3G44050.1 | 3.9e-156 | 38.11 | P-loop containing nucleoside triphosphate hydrolases superfamily protein | [more] |
AT3G23670.1 | 4.4e-99 | 52.80 | phragmoplast-associated kinesin-related protein, putative | [more] |
AT3G23670.2 | 4.4e-99 | 52.80 | phragmoplast-associated kinesin-related protein, putative | [more] |