Sgr025486 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025486
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionS5A_REDUCTASE domain-containing protein
Locationtig00006406: 1836345 .. 1837713 (+)
RNA-Seq ExpressionSgr025486
SyntenySgr025486
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATAGGGAGCAATCAGCTGTGGAAAGCGTTGGCATATGCGAACCTGCGGACCAAGATTTTGACAGGAGACGAAAGTTGGATCCATGGGTGGCGGCAGAAGTGACGGTAATCTGAAAAATGCCCTCATAGCATTACTAGTTCCGCTTCCCTCCGTTCTCTTTTACCTCTCTTTCCTCAGCCACAGCAGTAGTGGAAATCTTTCTTTTCTCTGGAAATGGTGCTATCACCATCCTCTTTTATTGGCCAATGCTCTCTTCTTCCTCAATGTCAACCTTGTCTTCTGGCTCATCAGTCATGTCCAGTCCAGCCACTGGGTGAGCCTTCTCCACTTGTCGTCTGTCGATTCCTAGAATCCTTCTCAATTTCTCAGCTTAACCAGCACGGTTGATTTTTAAAAATAAAAAAAAAAAATTGGTTAAAAGTTCCGCTGGTTTTTAAAATTTTTTTGGTGCAGATGATTGATTTGTACTGGACATTGATACCCGTGATGCTCGTTCACTATTTTGCTTCTCACCCTTTAGCTCAGTATAATCAATTGAGGTCATGGATCGCAATTGCTTTGACTTGGATCTGGAGCATCAGGCTTTCCCACAACTACTTCAGGCGGGAAACGTGGCAGTGGGGTGCGCGAGAGGACTGGAGGTTCACGAATATGCGCCGACAGTACGGCAAGAACTGGTGGTGGGTTTCTTTCTTCGCCGTTTACCTTTCACAGCAGGTAATAAATAACTAATAACTTGTTCCCAATCCAGTCATTCAATTCTCCTTCATGCCCAGGCTTTAATTACTTATAACCTGCTCGATGTTTAGCCGCAAGCCAGGTGGTTGATTCATGTTTGTTTCAACTTTCAACCTTAATAAATAGGTATTTCTGATAGGCGTATGCCTCCCTCTGTACGTTGTCCACTCAGTCGATGAGGCATTGAAGATGTGGGATTTCGTAGCCATCTTTGTGTGTGCTTCTGGCATTGTCATCGCGTACTTTGCTGACACACAACTCCATGAATTCGTGAGTAGAAACAGCAAGCTCAAAATGAGTGGAAAACCAATGGTGGCCAATCTTGAGGAGGGACTGTGGCGTTACTCTCGACACCCAAACTACTTTGGCGAGCAGTTGTGGTGGTGGGGACTTGTAATATTTGGGTGGAGTTTAGGTGAGGGATGGACCCTTGTTGGATCACTCATCAACAGTATGTGCTTGGCTTATGTAACCAAGCTGGTTGAAGAGCGAATGGTGAAGCAAGACTACAGAGCAGAAGCTTACAGGCAATATCAGAAGACTACATCAGTGTGGATACCTTGGTTCAAGGCATCAGTAACCGATGACGACGATGATGATGCTGATGAAAAGGAGAAGAACATGTGA

mRNA sequence

ATGGATAGGGAGCAATCAGCTGTGGAAAGCGTTGGCATATGCGAACCTGCGGACCAAGATTTTGACAGGAGACGAAAGTTGGATCCATGGGTGGCGGCAGAAGTGACGATGATTGATTTGTACTGGACATTGATACCCGTGATGCTCGTTCACTATTTTGCTTCTCACCCTTTAGCTCAGTATAATCAATTGAGGTCATGGATCGCAATTGCTTTGACTTGGATCTGGAGCATCAGGCTTTCCCACAACTACTTCAGGCGGGAAACGTGGCAGTGGGGTGCGCGAGAGGACTGGAGGTTCACGAATATGCGCCGACAGTACGGCAAGAACTGGTGGTGGGTTTCTTTCTTCGCCGTTTACCTTTCACAGCAGGTATTTCTGATAGGCGTATGCCTCCCTCTGTACGTTGTCCACTCAGTCGATGAGGCATTGAAGATGTGGGATTTCGTAGCCATCTTTGTGTGTGCTTCTGGCATTGTCATCGCGTACTTTGCTGACACACAACTCCATGAATTCGTGAGTAGAAACAGCAAGCTCAAAATGAGTGGAAAACCAATGGTGGCCAATCTTGAGGAGGGACTGTGGCGTTACTCTCGACACCCAAACTACTTTGGCGAGCAGTTGTGGTGGTGGGGACTTGTAATATTTGGGTGGAGTTTAGGTGAGGGATGGACCCTTGTTGGATCACTCATCAACAGTATGTGCTTGGCTTATGTAACCAAGCTGGTTGAAGAGCGAATGGTGAAGCAAGACTACAGAGCAGAAGCTTACAGGCAATATCAGAAGACTACATCAGTGTGGATACCTTGGTTCAAGGCATCAGTAACCGATGACGACGATGATGATGCTGATGAAAAGGAGAAGAACATGTGA

Coding sequence (CDS)

ATGGATAGGGAGCAATCAGCTGTGGAAAGCGTTGGCATATGCGAACCTGCGGACCAAGATTTTGACAGGAGACGAAAGTTGGATCCATGGGTGGCGGCAGAAGTGACGATGATTGATTTGTACTGGACATTGATACCCGTGATGCTCGTTCACTATTTTGCTTCTCACCCTTTAGCTCAGTATAATCAATTGAGGTCATGGATCGCAATTGCTTTGACTTGGATCTGGAGCATCAGGCTTTCCCACAACTACTTCAGGCGGGAAACGTGGCAGTGGGGTGCGCGAGAGGACTGGAGGTTCACGAATATGCGCCGACAGTACGGCAAGAACTGGTGGTGGGTTTCTTTCTTCGCCGTTTACCTTTCACAGCAGGTATTTCTGATAGGCGTATGCCTCCCTCTGTACGTTGTCCACTCAGTCGATGAGGCATTGAAGATGTGGGATTTCGTAGCCATCTTTGTGTGTGCTTCTGGCATTGTCATCGCGTACTTTGCTGACACACAACTCCATGAATTCGTGAGTAGAAACAGCAAGCTCAAAATGAGTGGAAAACCAATGGTGGCCAATCTTGAGGAGGGACTGTGGCGTTACTCTCGACACCCAAACTACTTTGGCGAGCAGTTGTGGTGGTGGGGACTTGTAATATTTGGGTGGAGTTTAGGTGAGGGATGGACCCTTGTTGGATCACTCATCAACAGTATGTGCTTGGCTTATGTAACCAAGCTGGTTGAAGAGCGAATGGTGAAGCAAGACTACAGAGCAGAAGCTTACAGGCAATATCAGAAGACTACATCAGTGTGGATACCTTGGTTCAAGGCATCAGTAACCGATGACGACGATGATGATGCTGATGAAAAGGAGAAGAACATGTGA

Protein sequence

MDREQSAVESVGICEPADQDFDRRRKLDPWVAAEVTMIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGAREDWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCASGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIFGWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVTDDDDDDADEKEKNM
Homology
[Close]
BLAST of Sgr025486 vs. NCBI nr
Match: XP_022146917.1 (uncharacterized protein C594.04c [Momordica charantia])

HSP 1 Score: 487.3 bits (1253), Expect = 9.4e-134
Identity = 229/254 (90.16%), Postives = 240/254 (94.49%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLAQYN+LRSWIAIALTWIWSIRLSHNYFRRE WQWGA+E
Sbjct: 79  MIDLYWTLIPVMLVHYFASHPLAQYNELRSWIAIALTWIWSIRLSHNYFRRERWQWGAKE 138

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT++RRQYG+NWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWD VAIFVC 
Sbjct: 139 DWRFTDLRRQYGENWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDLVAIFVCV 198

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFVSRNSKLK SGKPMVANLEEGLW YSRHPNYFGEQLWWWGLVIF
Sbjct: 199 SGIVIAYFADTQLHEFVSRNSKLKXSGKPMVANLEEGLWGYSRHPNYFGEQLWWWGLVIF 258

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            WSLG+GW+ VGSLINS+CLAYVTKLVEERMVKQ+YRAEAYR+YQKTTSVWIPWFK S+T
Sbjct: 259 AWSLGKGWSFVGSLINSLCLAYVTKLVEERMVKQEYRAEAYRKYQKTTSVWIPWFKTSLT 318

Query: 277 DDDDDDADEKEKNM 291
                 ADEKEKNM
Sbjct: 319 ------ADEKEKNM 326

[Close]
BLAST of Sgr025486 vs. NCBI nr
Match: XP_023521961.1 (uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo] >XP_023538958.1 uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 219/254 (86.22%), Postives = 232/254 (91.34%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA YN+LRSWIAIALTWIWSIRL+HNY RRE WQ G RE
Sbjct: 80  MIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVRE 139

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVYLSQQ+FLIGVCLPLYVVHSVDEAL MWD VAIFVC 
Sbjct: 140 DWRFTDMRQQYGKNWWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCV 199

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFV+RNS+LKMSGK MV NLEEGLWRYSRHPNYFGEQLWWWGLVIF
Sbjct: 200 SGIVIAYFADTQLHEFVTRNSELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIF 259

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            W LGE W+LVGS++NS+CLAYVTKLVEERMVKQ+YRAEAYRQYQKTTSVWIPWF  SV 
Sbjct: 260 AWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVK 319

Query: 277 DDDDDDADEKEKNM 291
           D      DEK+KNM
Sbjct: 320 D------DEKDKNM 327

[Close]
BLAST of Sgr025486 vs. NCBI nr
Match: XP_023521436.1 (uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 219/254 (86.22%), Postives = 232/254 (91.34%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA YN+LRSWIAIALTWIWSIRL+HNY RRE WQ G RE
Sbjct: 80  MIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVRE 139

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVYLSQQ+FLIGVCLPLYVVHSVDEAL MWD VAIFVC 
Sbjct: 140 DWRFTDMRQQYGKNWWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCV 199

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFV+RNS+LKMSGK MV NLEEGLWRYSRHPNYFGEQLWWWGLVIF
Sbjct: 200 SGIVIAYFADTQLHEFVTRNSELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIF 259

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            W LGE W+LVGS++NS+CLAYVTKLVEERMVKQ+YRAEAYRQYQKTTSVWIPWF  SV 
Sbjct: 260 AWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVK 319

Query: 277 DDDDDDADEKEKNM 291
           D      DEK+KNM
Sbjct: 320 D------DEKDKNM 327

[Close]
BLAST of Sgr025486 vs. NCBI nr
Match: XP_023522567.1 (uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 466.8 bits (1200), Expect = 1.3e-127
Identity = 219/254 (86.22%), Postives = 232/254 (91.34%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA YN+LRSWIAIALTWIWSIRL+HNY RRE WQ G RE
Sbjct: 1   MIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVRE 60

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVYLSQQ+FLIGVCLPLYVVHSVDEAL MWD VAIFVC 
Sbjct: 61  DWRFTDMRQQYGKNWWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCM 120

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFV+RNS+LKMSGK MV NLEEGLWRYSRHPNYFGEQLWWWGLVIF
Sbjct: 121 SGIVIAYFADTQLHEFVTRNSELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIF 180

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            W LGE W+LVGS++NS+CLAYVTKLVEERMVKQ+YRAEAYRQYQKTTSVWIPWF  SV 
Sbjct: 181 AWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVK 240

Query: 277 DDDDDDADEKEKNM 291
           D      DEK+KNM
Sbjct: 241 D------DEKDKNM 248

[Close]
BLAST of Sgr025486 vs. NCBI nr
Match: KAG7028469.1 (Transcription factor 25 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 466.1 bits (1198), Expect = 2.2e-127
Identity = 218/254 (85.83%), Postives = 232/254 (91.34%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA YN+LRSWIAIALTWIWSIRL+HNY RRE WQ G RE
Sbjct: 730 MIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVRE 789

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVYLSQQ+FLIGVCLPLYVVHSVDEAL MWD VAIFVC 
Sbjct: 790 DWRFTDMRQQYGKNWWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCV 849

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFV+RN++LKMSGK MV NLEEGLWRYSRHPNYFGEQLWWWGLVIF
Sbjct: 850 SGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIF 909

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            W LGE W+LVGS++NS+CLAYVTKLVEERMVKQ+YRAEAYRQYQKTTSVWIPWF  SV 
Sbjct: 910 AWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVK 969

Query: 277 DDDDDDADEKEKNM 291
           D      DEK+KNM
Sbjct: 970 D------DEKDKNM 977

[Close]
BLAST of Sgr025486 vs. ExPASy Swiss-Prot
Match: O74507 (Uncharacterized protein C594.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPCC594.04c PE=3 SV=2)

HSP 1 Score: 73.9 bits (180), Expect = 3.3e-12
Identity = 74/277 (26.71%), Postives = 120/277 (43.32%), Query Frame = 0 

Query: 36  TMIDLYWTLIPVMLVHYFASHPLAQYN--QLRSWIAIALTWIWSIRLSHNYFRRETWQWG 95
           + +D  W ++P     +F  + L  YN    R  I   L  +WS RL++NY+R+  +  G
Sbjct: 78  SQVDRLWPILPTAFSLHFLFYGLG-YNIASRRLMIMAFLQTLWSARLTYNYYRKGGYNRG 137

Query: 96  AREDWRFTNMRRQYGKNWWWV----SFFAVYLSQQVFLIGVCLPLYV--VHSVDEALKMW 155
           A ED+R+  +R+   K   W+     +F +++ Q + L  +  P Y+  +   + A   W
Sbjct: 138 A-EDYRWVRVRQIMPK---WIYPLFHYFYIHIFQVLHLYLLASPTYIAMLAGNERAFGAW 197

Query: 156 DFVAIFVCASGIVIAYFADTQLHEFVSRNSKLKMS--------------GKPMVANLEEG 215
           D++A+ +     V+   AD Q  ++    +   +               G+   A    G
Sbjct: 198 DWIALELFMFMFVLEMLADQQQWDYYEARNHYNVDKTVPPRFKYDLLSLGRGFNAT---G 257

Query: 216 LWRYSRHPNYFGEQLWWWGLVIFGWSLGE---GWTLVGSLINSMCLAYVTKLVEERMVKQ 275
           L+R+SRHPN+  EQL W    +FG    E    WT+   L         T+L E+   + 
Sbjct: 258 LFRWSRHPNFLAEQLIWLSFYLFGAIASESLLNWTIFAWLGLVGVFQGSTRLTEKMSCE- 317

Query: 276 DYRAEAYRQYQKTTSVWIPWFKASVTDDDDDDADEKE 288
             +   YR YQ     + P    S  D  DDDA  KE
Sbjct: 318 --KYPLYRVYQDKVGRFFPRLDGSHWDIVDDDASLKE 343

[Close]
BLAST of Sgr025486 vs. ExPASy TrEMBL
Match: A0A6J1D0X1 (uncharacterized protein C594.04c OS=Momordica charantia OX=3673 GN=LOC111015999 PE=4 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.6e-134
Identity = 229/254 (90.16%), Postives = 240/254 (94.49%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLAQYN+LRSWIAIALTWIWSIRLSHNYFRRE WQWGA+E
Sbjct: 79  MIDLYWTLIPVMLVHYFASHPLAQYNELRSWIAIALTWIWSIRLSHNYFRRERWQWGAKE 138

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT++RRQYG+NWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWD VAIFVC 
Sbjct: 139 DWRFTDLRRQYGENWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDLVAIFVCV 198

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFVSRNSKLK SGKPMVANLEEGLW YSRHPNYFGEQLWWWGLVIF
Sbjct: 199 SGIVIAYFADTQLHEFVSRNSKLKXSGKPMVANLEEGLWGYSRHPNYFGEQLWWWGLVIF 258

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            WSLG+GW+ VGSLINS+CLAYVTKLVEERMVKQ+YRAEAYR+YQKTTSVWIPWFK S+T
Sbjct: 259 AWSLGKGWSFVGSLINSLCLAYVTKLVEERMVKQEYRAEAYRKYQKTTSVWIPWFKTSLT 318

Query: 277 DDDDDDADEKEKNM 291
                 ADEKEKNM
Sbjct: 319 ------ADEKEKNM 326

[Close]
BLAST of Sgr025486 vs. ExPASy TrEMBL
Match: A0A6J1I9U8 (uncharacterized protein C594.04c OS=Cucurbita maxima OX=3661 GN=LOC111472914 PE=4 SV=1)

HSP 1 Score: 463.0 bits (1190), Expect = 9.2e-127
Identity = 215/254 (84.65%), Postives = 231/254 (90.94%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA YN+LRSWIAIALTWIWSIRL+HNY RRE WQ G RE
Sbjct: 80  MIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAIALTWIWSIRLTHNYLRREGWQLGVRE 139

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVY SQQ+FL+GVCLPLYVVHSVDEAL +WD VAIFVC 
Sbjct: 140 DWRFTDMRQQYGKNWWWVSFFAVYFSQQIFLVGVCLPLYVVHSVDEALNLWDLVAIFVCV 199

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFV+RN++LKMSGK MV NLEEGLWRYSRHPNYFGEQLWWWGLVIF
Sbjct: 200 SGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIF 259

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            W LGE W+LVGS++NS+CLAYVTKLVEERMVKQ+YRAEAYRQYQKTTSVWIPWF  SV 
Sbjct: 260 AWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVK 319

Query: 277 DDDDDDADEKEKNM 291
           D      DEK+KNM
Sbjct: 320 D------DEKDKNM 327

[Close]
BLAST of Sgr025486 vs. ExPASy TrEMBL
Match: A0A6J1F9X4 (uncharacterized protein C594.04c OS=Cucurbita moschata OX=3662 GN=LOC111442154 PE=4 SV=1)

HSP 1 Score: 458.4 bits (1178), Expect = 2.3e-125
Identity = 211/242 (87.19%), Postives = 225/242 (92.98%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA YN+LRSWIAI+LTWIWSIRL+HNY RRE WQ G RE
Sbjct: 80  MIDLYWTLIPVMLVHYFASHPLAHYNELRSWIAISLTWIWSIRLTHNYLRREGWQLGVRE 139

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVYLSQQ+FLIGVCLPLYVVHSVDEAL MWD VAIFVC 
Sbjct: 140 DWRFTDMRQQYGKNWWWVSFFAVYLSQQIFLIGVCLPLYVVHSVDEALNMWDLVAIFVCV 199

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGIVIAYFADTQLHEFV+RN++LKMSGK MV NLEEGLWRYSRHPNYFGEQLWWWGLVIF
Sbjct: 200 SGIVIAYFADTQLHEFVTRNNELKMSGKSMVPNLEEGLWRYSRHPNYFGEQLWWWGLVIF 259

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
            W LGE W+LVGS++NS+CLAYVTKLVEERMVKQ+YRAEAYRQYQKTTSVWIPWF  SV 
Sbjct: 260 AWGLGEEWSLVGSMVNSLCLAYVTKLVEERMVKQEYRAEAYRQYQKTTSVWIPWFNTSVK 319

Query: 277 DD 279
            D
Sbjct: 320 KD 321

[Close]
BLAST of Sgr025486 vs. ExPASy TrEMBL
Match: A0A0A0L2V4 (S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G116670 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.7e-120
Identity = 205/254 (80.71%), Postives = 223/254 (87.80%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPVMLVHYFASHPLA+YN+LRSW+ +ALTWIWSIRLSHNY RRE WQ G RE
Sbjct: 80  MIDLYWTLIPVMLVHYFASHPLAEYNELRSWVVVALTWIWSIRLSHNYLRREGWQLGTRE 139

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYGKNWWWVSFFAVYLSQQVFL+GVC+PLYVVHSV E LK+WD VAIF+C 
Sbjct: 140 DWRFTDMRQQYGKNWWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFICV 199

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGI +AYFADTQLHEFVSRN KLKMSGK MV NLEEGLWRY RHPNYFGEQLWWWG+ I 
Sbjct: 200 SGIGMAYFADTQLHEFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGIL 259

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
           GW LG GW+LVGSL+N+MCLAYVTKLVE RMVKQ YRAEAYRQYQKTTSVWIPWF+ S+ 
Sbjct: 260 GWGLGVGWSLVGSLLNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSL- 319

Query: 277 DDDDDDADEKEKNM 291
                    K+KNM
Sbjct: 320 --------YKQKNM 324

[Close]
BLAST of Sgr025486 vs. ExPASy TrEMBL
Match: A0A5D3DAX2 (S5A_REDUCTASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00100 PE=4 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.5e-118
Identity = 202/254 (79.53%), Postives = 222/254 (87.40%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MIDLYWTLIPV+LVHYFASHPLA+YN+LRSW+ +ALTWIWS RLSHNY RRE WQ GARE
Sbjct: 81  MIDLYWTLIPVVLVHYFASHPLAEYNELRSWVVVALTWIWSTRLSHNYLRREGWQLGARE 140

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRFT+MR+QYG+NWWWVSFFAVYLSQQVFL+GVC+PLYVVHSV E LK+WD VAIF+C 
Sbjct: 141 DWRFTDMRQQYGRNWWWVSFFAVYLSQQVFLMGVCVPLYVVHSVKEELKLWDLVAIFMCV 200

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           SGI +AYFADTQLHEFVSRN KLKMSGK MV NLEEGLWRY RHPNYFGEQLWWWG+ I 
Sbjct: 201 SGIGMAYFADTQLHEFVSRNRKLKMSGKAMVPNLEEGLWRYCRHPNYFGEQLWWWGVGIL 260

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFKASVT 276
           GW LG GW+ VGSL+N+MCLAYVTKLVE RMVKQ YRAEAYRQYQKTTSVWIPWF+ S+ 
Sbjct: 261 GWGLGVGWSPVGSLLNTMCLAYVTKLVEARMVKQHYRAEAYRQYQKTTSVWIPWFRKSL- 320

Query: 277 DDDDDDADEKEKNM 291
                    K+KNM
Sbjct: 321 --------YKQKNM 325

[Close]
BLAST of Sgr025486 vs. TAIR 10
Match: AT2G46890.1 (Protein of unknown function (DUF1295) )

HSP 1 Score: 417.5 bits (1072), Expect = 8.5e-117
Identity = 182/244 (74.59%), Postives = 216/244 (88.52%), Query Frame = 0 

Query: 37  MIDLYWTLIPVMLVHYFASHPLAQYNQLRSWIAIALTWIWSIRLSHNYFRRETWQWGARE 96
           MID+YWT+IPVMLVHYFASHPL+QYN+LRS I I LTWIWSIRL+HNYFRRE W+WGARE
Sbjct: 76  MIDVYWTVIPVMLVHYFASHPLSQYNKLRSMIVITLTWIWSIRLTHNYFRRENWEWGARE 135

Query: 97  DWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVDEALKMWDFVAIFVCA 156
           DWRF ++R+QYGK+WWW+SFF+VY+SQQ+FLIG+CLPLYV+HS+DE L +WDF++  +C 
Sbjct: 136 DWRFNDLRKQYGKHWWWLSFFSVYVSQQIFLIGICLPLYVIHSIDEPLNIWDFISSAICL 195

Query: 157 SGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIF 216
           +GIV+AYFADTQLHEFV+ N KLK  GKP + NL+ GLWRYSRHPNY GEQLWWWGLVIF
Sbjct: 196 TGIVMAYFADTQLHEFVTGNQKLKEQGKPKIPNLDSGLWRYSRHPNYLGEQLWWWGLVIF 255

Query: 217 GWSLGEGWTLVGSLINSMCLAYVTKLVEERMVKQDYRAEAYRQYQKTTSVWIPWFK--AS 276
            W+LG+GWTL+G+L+N++CL YVT LVE RMVKQ YRAEAYR YQKTTSVWIPWFK  A+
Sbjct: 256 AWNLGQGWTLIGALVNTLCLVYVTILVERRMVKQQYRAEAYRAYQKTTSVWIPWFKSHAA 315

Query: 277 VTDD 279
            T D
Sbjct: 316 ATKD 319

[Close]
BLAST of Sgr025486 vs. TAIR 10
Match: AT1G18180.1 (Protein of unknown function (DUF1295) )

HSP 1 Score: 75.9 bits (185), Expect = 6.1e-14
Identity = 65/212 (30.66%), Postives = 100/212 (47.17%), Query Frame = 0 

Query: 65  RSWIAIALTWIWSIRLSHNYFRRETWQWGAREDWRFTNMRRQYGKNWWWVSFFAVYLSQQ 124
           R  +   L  +W +RL   +      QWG  ED RF +MR   GK          ++ Q 
Sbjct: 62  RQIVLTVLVVVWGLRLG-LFLLMRILQWG--EDRRFDDMRENIGK------LVVFWIFQA 121

Query: 125 VFLIGVCLPLYVVHSVD--EALKMWDFVAIFVCASGIVIAYFADTQLHEFVSRNSKLKMS 184
           V++  V LP+  V++ +     +  D +   +  +G +I   AD Q           K+S
Sbjct: 122 VWVWTVSLPVTFVNASNGGRLFQPADVIGWTMWVAGFLIEATADQQ-----------KLS 181

Query: 185 GKPMVAN----LEEGLWRYSRHPNYFGEQLWWWGLVIFGWSLGEG---WTLVGSLINSMC 244
            K    N     + G+W+YSRHPNYFGE L WWGL +    + +G     ++G +  ++ 
Sbjct: 182 FKKCPENKGKWCDVGVWKYSRHPNYFGEMLLWWGLYVASLPVLKGLEYLVIIGPVFLTLL 241

Query: 245 LAYVT--KLVEERMVKQDYRAEAYRQYQKTTS 266
           L +V+   L+EE   K+     AYR Y+KTTS
Sbjct: 242 LLFVSGIPLLEESADKKYGNLGAYRHYKKTTS 253

[Close]
BLAST of Sgr025486 vs. TAIR 10
Match: AT1G73650.2 (Protein of unknown function (DUF1295) )

HSP 1 Score: 73.9 bits (180), Expect = 2.3e-13
Identity = 66/208 (31.73%), Postives = 96/208 (46.15%), Query Frame = 0 

Query: 65  RSWIAIALTWIWSIRLSHNYFRRETWQWGAREDWRFTNMRRQYGKNWWWVSFFAVYLSQQ 124
           R  +   L  +W +RL   +      QWG  ED RF   R         V     +  Q 
Sbjct: 62  RQIVLTLLVVVWGLRLG-IFLLMRILQWG--EDRRFDEQRGNI------VRLIIFWTLQA 121

Query: 125 VFLIGVCLPLYVVHSVD--EALKMWDFVAIFVCASGIVIAYFADTQLHEFVSRNSKLKMS 184
           V++  V LPL +V++ D   +LK  D +   +   G +I   AD Q   F       K S
Sbjct: 122 VWVWTVSLPLTLVNASDGGGSLKPADVIGWTMWVFGFLIEAAADQQKLSF-------KNS 181

Query: 185 GKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIFGWSLGEG---WTLVGSLINSMCLAYV 244
            +      + G+W+YSRHPNYFGE L WWG+ +    + EG     + G L  ++ L +V
Sbjct: 182 PENRGKWCDVGVWKYSRHPNYFGEMLLWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFV 241

Query: 245 T--KLVEERMVKQDYRAEAYRQYQKTTS 266
           +   L+E    K+   + AYR Y+KTTS
Sbjct: 242 SGIPLLEASADKKHGNSGAYRSYKKTTS 253

[Close]
BLAST of Sgr025486 vs. TAIR 10
Match: AT1G73650.3 (Protein of unknown function (DUF1295) )

HSP 1 Score: 73.9 bits (180), Expect = 2.3e-13
Identity = 66/208 (31.73%), Postives = 96/208 (46.15%), Query Frame = 0 

Query: 65  RSWIAIALTWIWSIRLSHNYFRRETWQWGAREDWRFTNMRRQYGKNWWWVSFFAVYLSQQ 124
           R  +   L  +W +RL   +      QWG  ED RF   R         V     +  Q 
Sbjct: 62  RQIVLTLLVVVWGLRLG-IFLLMRILQWG--EDRRFDEQRGNI------VRLIIFWTLQA 121

Query: 125 VFLIGVCLPLYVVHSVD--EALKMWDFVAIFVCASGIVIAYFADTQLHEFVSRNSKLKMS 184
           V++  V LPL +V++ D   +LK  D +   +   G +I   AD Q   F       K S
Sbjct: 122 VWVWTVSLPLTLVNASDGGGSLKPADVIGWTMWVFGFLIEAAADQQKLSF-------KNS 181

Query: 185 GKPMVANLEEGLWRYSRHPNYFGEQLWWWGLVIFGWSLGEG---WTLVGSLINSMCLAYV 244
            +      + G+W+YSRHPNYFGE L WWG+ +    + EG     + G L  ++ L +V
Sbjct: 182 PENRGKWCDVGVWKYSRHPNYFGEMLLWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFV 241

Query: 245 T--KLVEERMVKQDYRAEAYRQYQKTTS 266
           +   L+E    K+   + AYR Y+KTTS
Sbjct: 242 SGIPLLEASADKKHGNSGAYRSYKKTTS 253

[Close]
BLAST of Sgr025486 vs. TAIR 10
Match: AT1G73650.1 (Protein of unknown function (DUF1295) )

HSP 1 Score: 70.9 bits (172), Expect = 2.0e-12
Identity = 61/182 (33.52%), Postives = 87/182 (47.80%), Query Frame = 0 

Query: 91  QWGAREDWRFTNMRRQYGKNWWWVSFFAVYLSQQVFLIGVCLPLYVVHSVD--EALKMWD 150
           QWG  ED RF   R         V     +  Q V++  V LPL +V++ D   +LK  D
Sbjct: 5   QWG--EDRRFDEQRGNI------VRLIIFWTLQAVWVWTVSLPLTLVNASDGGGSLKPAD 64

Query: 151 FVAIFVCASGIVIAYFADTQLHEFVSRNSKLKMSGKPMVANLEEGLWRYSRHPNYFGEQL 210
            +   +   G +I   AD Q   F       K S +      + G+W+YSRHPNYFGE L
Sbjct: 65  VIGWTMWVFGFLIEAAADQQKLSF-------KNSPENRGKWCDVGVWKYSRHPNYFGEML 124

Query: 211 WWWGLVIFGWSLGEG---WTLVGSLINSMCLAYVT--KLVEERMVKQDYRAEAYRQYQKT 266
            WWG+ +    + EG     + G L  ++ L +V+   L+E    K+   + AYR Y+KT
Sbjct: 125 LWWGIFVAASPVLEGAEYLVIFGPLFLTLLLLFVSGIPLLEASADKKHGNSGAYRSYKKT 171

The following BLAST results are available for this feature:
BLAST of Sgr025486 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022146917.19.4e-13490.16uncharacterized protein C594.04c [Momordica charantia][more]
XP_023521961.11.0e-12786.22uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo] >XP_023538958... [more]
XP_023521436.11.0e-12786.22uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo][more]
XP_023522567.11.3e-12786.22uncharacterized protein C594.04c-like [Cucurbita pepo subsp. pepo][more]
KAG7028469.12.2e-12785.83Transcription factor 25 [Cucurbita argyrosperma subsp. argyrosperma][more]
BLAST of Sgr025486 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O745073.3e-1226.71Uncharacterized protein C594.04c OS=Schizosaccharomyces pombe (strain 972 / ATCC... [more]
BLAST of Sgr025486 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1D0X14.6e-13490.16uncharacterized protein C594.04c OS=Momordica charantia OX=3673 GN=LOC111015999 ... [more]
A0A6J1I9U89.2e-12784.65uncharacterized protein C594.04c OS=Cucurbita maxima OX=3661 GN=LOC111472914 PE=... [more]
A0A6J1F9X42.3e-12587.19uncharacterized protein C594.04c OS=Cucurbita moschata OX=3662 GN=LOC111442154 P... [more]
A0A0A0L2V43.7e-12080.71S5A_REDUCTASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G1166... [more]
A0A5D3DAX23.5e-11879.53S5A_REDUCTASE domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 G... [more]
BLAST of Sgr025486 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G46890.18.5e-11774.59Protein of unknown function (DUF1295) [more]
AT1G18180.16.1e-1430.66Protein of unknown function (DUF1295) [more]
AT1G73650.22.3e-1331.73Protein of unknown function (DUF1295) [more]
AT1G73650.32.3e-1331.73Protein of unknown function (DUF1295) [more]
AT1G73650.12.0e-1233.52Protein of unknown function (DUF1295) [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010721Protein of unknown function DUF1295PFAMPF06966DUF1295coord: 34..264
e-value: 4.3E-66
score: 222.7
IPR010721Protein of unknown function DUF1295PANTHERPTHR322513-OXO-5-ALPHA-STEROID 4-DEHYDROGENASEcoord: 35..273
NoneNo IPR availableGENE3D1.20.120.1630coord: 66..271
e-value: 1.1E-23
score: 86.0
NoneNo IPR availablePANTHERPTHR32251:SF233-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE (DUF1295)coord: 35..273
IPR0011043-oxo-5-alpha-steroid 4-dehydrogenase, C-terminalPROSITEPS50244S5A_REDUCTASEcoord: 151..220
score: 9.989579

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025486.1Sgr025486.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0016627 oxidoreductase activity, acting on the CH-CH group of donors