Sgr025443 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr025443
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	cytochrome b5-like
Location	tig00006406: 1371766 .. 1374684 (+)
RNA-Seq Expression	Sgr025443
Synteny	Sgr025443

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGGAAAGGCGATGAGAAGAAATCGTGCCCACAAGGATCGTGTTTTCAACTTCCTTCTTCCAGGTATCCACTGTAAGAAACTTGGCTTCTTCCCCATTTATTCAGTCTTTTCATCGTCTGATTATTCTATCGAATAACTTCCTGCTGGATTAATCGTTTGCTTTCACTAGGGATTGAGATTGAGTTTCGTTGGCAATTGGATTGTGGCAGAAAATGGCTTCAGATCCTAAGGTTTTCGTCTTCGACGAAGTGGCAAAACACAATCACCTGCGGGATTGCTGGATTATAATCTCTGGAAAGGTCTCTCTTCTGTTCATCTCTCTCGTTTTCTTTCTTTCATCTTCGTTTGGGTATTTTCGGCTACTGTTTTGGGGTATTTTGTAATGTAAACTCAATTTGGTAAATCATTTATTCGTTCAGAACTCAAATCACCAAAAACAAACTATTTTGCGGAAGTAAATTTTCAAATCTTCATTTCTTTTTCTTCCCACACTACCTTCCTCACATATTCTCTAACTCTCTTTCTCCAACATACCTCTCTTTCTAACTTTGATGTCTCCCCTCTAAATTCTCTCTTTTTCCTATTTTTTAGCTTATACGTTAAATCCTAACACTTTCAGTTATGTCTATTTAGTTTATGTTTTTAAAAATTTTTAATAAACTCCAAAACTTTTATGGTGTGTTTACTTATTTTTAATAGTATAAAAAAATTCTAATAACTTTCTAAACTTTTAATTTTATATCTAATAAATACATGTCATTACCTTCATTAGTTTAACACTTATGTGGCATGTTGATTCTTTAAGTATTACGTCCAAAATTAGCCCGTTACATGATTTTTAAAAAATATAATGTTATTTTGTCATTTAAGTGATAAGTGAATTAAGTTCAGGAATCTATTAGACACAAAAATGAAAATTCATTCAAAAATCTATTATAAACTTTAAGAGTATGAAGACTAAATAAATACAACCCTAAAAATTTAAAGACCAAACTTGTAATTTAATCTATTTTTTCTCTCTTACTTTTCTTACTTTTCTTATCTACAATTTATCTTTCTCCCTAGTTTTTATCCTTTACAAAACTTTGCTTTCTCTCTCCAACTTATCTTCCTTGCTCTCACATATACTTCTCTCTCTAAAAATCTCTTTCTCTAAAATCTCCATCTCCATCTCCATCTCTATACCTTTTCTTTCTCTTTTCTCCAACTTATTTCTTTTTCTCCAACTTATTTCTTTTTCTCAAACATATCTTACTTTTCTCTCTCTAAAACTTTTATCCCTCCATAAATTTTCTCTCTCTAACTTATCTTTCTTTCACTAGCATCTCTCCCTCTTTTGTTAACTTTTCTCTCTCTACACGTTTCCCTCTAAAACTTTTATCTTTGGCTCTCTAATGTATGTTTCTTTTTAACATAACTCTTGCTTTCTTACTTTTATATCTATGTCGTCTGTTTCCCAAGATTTTGAGGGATTCCATTGTTTGTTATCCTTTAAAATAGAAGATTTTACTATATTTTACAAATTTTAAATTAAATTACTAATTTCACCTTATCATATTTTACATCAAGAGTGGAAGGTTATATTAGTAAACCTTTTATTTTATTTTTTTATTCTAGCAACATTCAAATAAAAAATTTTAATTACATTATTTAAAATTTTTCTTATTCCAAACAAGATAAAGAATTTAAAATCATTTTCAAAAACCCAAATCACAAGATTTAAAATCATTTTTTTATACTTTCTTGATTTCAAGCGGAGGGCAAAATTTTCTACTCTTTCTAAAATTTCTCTTTCTGTCTCTAACTTTTCACTCTCTCTAACAAAAGAAAATTGAATGGGTTGTCGAACAACCCTACTTTTCAAAAGTGAAAAACCAAAATAAAAATGAAATAATTATCAAACCACGCTAGAAAAACCAAAATAGTTATCAAGCAGACCCTTTGCTTTTCGAGTGAAACAGTAGAGACTTATATGAGAGCAAGAGAGATGGGAGATGAGAAATGTTTATTTCTTGATCTTCCTCGGAACAATCATTAGAAATTGAAGACAGTGTCCTGTGTTGTTATAATGACCTCCAAAATAGTGTCCTGTCTCTCGATCTTCCTTCGAATAATCTTTTCTTCCTCTTCTTCAAAGGCCAAATACTTGAACTGGGGCAAATTGTTTGTGATAGATAGGAAACTGAATGCTCAGAGAAAGCTCTACTGCAACTCTAGATTGCTGTTAGTTAGGACTAGAGACATTGTAGCCATGGCAACATATAATAATAGCACTAGAGTCAAATGGAGTTGCAGATCTAAGTTTTATCAGGGCTCTTTCGGTGTTTCTAGAAGGATCCTGAAAATATTTTTGCTGTTGTTATATCAGTACTCATCGCCAGATATACCAATAATCAGATCCTTACTACTTCGGTGACAGAATTTATACCTTTGTTTCATTGCTTTGCCCCATAGTAATTTGCACATTACCCATGTTAGGTATATGATGTCACCCCATTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTGCTATTAGCTGCTGGTAAGTTTCAGCTCATCATTTTTTTTCCATCTTGATAAATATCCAACAAAGATGATTGCACCAAGTTATGAATGATTGTAAAAACAATCTTGTCTTTATGCAGAAAAAGATGCAACTGATGATTTTGAAAATGTGGGTCACAGTCTAGCTGCAATAGAGCAAATGGAAAAATATTATGTTGGCAAGGTTGACATGTCTACCGTTCCAAAGCCGATCGATTATCGACCACCTGCATCCCATCCAGCAACCACCCCATCAGCTCAATCTTCAGGATCTTTGATCAGATTGCTGCAATTTCTGATTCCCCTGCTGATTTTGGGTGTTGCATTTTATCTGCAATTCTATGGCAAAAAACAGCAATCCAATTAA

mRNA sequence

ATGGTTGGAAAGGCGATGAGAAGAAATCGTGCCCACAAGGATCGTGTTTTCAACTTCCTTCTTCCAGATCCTAAGGTTTTCGTCTTCGACGAAGTGGCAAAACACAATCACCTGCGGGATTGCTGGATTATAATCTCTGGAAAGGTATATGATGTCACCCCATTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTGCTATTAGCTGCTGAAAAAGATGCAACTGATGATTTTGAAAATGTGGGTCACAGTCTAGCTGCAATAGAGCAAATGGAAAAATATTATGTTGGCAAGGTTGACATGTCTACCGTTCCAAAGCCGATCGATTATCGACCACCTGCATCCCATCCAGCAACCACCCCATCAGCTCAATCTTCAGGATCTTTGATCAGATTGCTGCAATTTCTGATTCCCCTGCTGATTTTGGGTGTTGCATTTTATCTGCAATTCTATGGCAAAAAACAGCAATCCAATTAA

Coding sequence (CDS)

ATGGTTGGAAAGGCGATGAGAAGAAATCGTGCCCACAAGGATCGTGTTTTCAACTTCCTTCTTCCAGATCCTAAGGTTTTCGTCTTCGACGAAGTGGCAAAACACAATCACCTGCGGGATTGCTGGATTATAATCTCTGGAAAGGTATATGATGTCACCCCATTTCTGGAAGAACATCCTGGAGGTGATGAAGTCTTGCTATTAGCTGCTGAAAAAGATGCAACTGATGATTTTGAAAATGTGGGTCACAGTCTAGCTGCAATAGAGCAAATGGAAAAATATTATGTTGGCAAGGTTGACATGTCTACCGTTCCAAAGCCGATCGATTATCGACCACCTGCATCCCATCCAGCAACCACCCCATCAGCTCAATCTTCAGGATCTTTGATCAGATTGCTGCAATTTCTGATTCCCCTGCTGATTTTGGGTGTTGCATTTTATCTGCAATTCTATGGCAAAAAACAGCAATCCAATTAA

Protein sequence

MVGKAMRRNRAHKDRVFNFLLPDPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLILGVAFYLQFYGKKQQSN

Homology

[Close]

BLAST of Sgr025443 vs. NCBI nr
Match: XP_022147466.1 (cytochrome b5-like [Momordica charantia])

HSP 1 Score: 241.5 bits (615), Expect = 4.9e-60
Identity = 115/134 (85.82%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVF FDEVAKHNH RDCW+IISGKVYDVTPFLEEHPGGDEVL+LA+EKDATDDFENVG
Sbjct: 4   DPKVFAFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLILASEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATT-PSAQSSGSLIRLLQFLIPLLI 142
           HSL+AI+QME+YYVG VDMSTVPKPIDYRPPAS  ATT  SAQSSGS ++LLQFLIPLL+
Sbjct: 64  HSLSAIDQMEQYYVGNVDMSTVPKPIDYRPPASQSATTLASAQSSGSSLKLLQFLIPLLL 123

Query: 143 LGVAFYLQFYGKKQ 156
           +GVAFY+QFYGKKQ
Sbjct: 124 IGVAFYMQFYGKKQ 137

[Close]

BLAST of Sgr025443 vs. NCBI nr
Match: XP_023540248.1 (cytochrome b5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 238.8 bits (608), Expect = 3.2e-59
Identity = 117/135 (86.67%), Postives = 124/135 (91.85%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASH--PATTPSAQSSGSLIRLLQFLIPLL 142
           HSL+AIEQMEKYY+G VDMSTVPKPIDYR  AS    ATT SA+SSGSL+ LLQ L+PLL
Sbjct: 64  HSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLL 123

Query: 143 ILGVAFYLQFYGKKQ 156
           I+GVAFYLQFYGKKQ
Sbjct: 124 IMGVAFYLQFYGKKQ 138

[Close]

BLAST of Sgr025443 vs. NCBI nr
Match: XP_022931217.1 (cytochrome B5-like [Cucurbita moschata] >XP_022974834.1 cytochrome B5-like [Cucurbita maxima] >XP_022974835.1 cytochrome B5-like [Cucurbita maxima])

HSP 1 Score: 235.7 bits (600), Expect = 2.7e-58
Identity = 116/135 (85.93%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVT FLEEHPGGDEVLLLAAEKDATDDFENVG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASH--PATTPSAQSSGSLIRLLQFLIPLL 142
           HSL+AIEQMEKYY+G VDMSTVPKPIDYR  AS    ATT SA+SSGSL+ LLQ L+PLL
Sbjct: 64  HSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLL 123

Query: 143 ILGVAFYLQFYGKKQ 156
           I+GVAFYLQFYGKKQ
Sbjct: 124 IMGVAFYLQFYGKKQ 138

[Close]

BLAST of Sgr025443 vs. NCBI nr
Match: XP_038901262.1 (cytochrome b5-like [Benincasa hispida])

HSP 1 Score: 235.7 bits (600), Expect = 2.7e-58
Identity = 115/141 (81.56%), Postives = 124/141 (87.94%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVTPFLEEHPGGDEVLLLA EKDATDDFE VG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLLLATEKDATDDFETVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPA--------TTPSAQSSGSLIRLLQ 142
           HSL+A E+MEKYYVG VDMSTVPKP+DYRP AS  A        TT SAQSSGSLI++LQ
Sbjct: 64  HSLSATEEMEKYYVGNVDMSTVPKPVDYRPAASQSAKASATATITTSSAQSSGSLIKVLQ 123

Query: 143 FLIPLLILGVAFYLQFYGKKQ 156
           +LIPLLI+GVAFYLQ+YGKKQ
Sbjct: 124 YLIPLLIIGVAFYLQYYGKKQ 144

[Close]

BLAST of Sgr025443 vs. NCBI nr
Match: XP_022975276.1 (cytochrome B5-like [Cucurbita maxima])

HSP 1 Score: 235.3 bits (599), Expect = 3.5e-58
Identity = 115/135 (85.19%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVT FLEEHPGGDEVLLLAAEKDATDDFENVG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASH--PATTPSAQSSGSLIRLLQFLIPLL 142
           HSL+AIEQMEKYY+G VDMST+PKPIDYR  AS    ATT SA+SSGSL+ LLQ L+PLL
Sbjct: 64  HSLSAIEQMEKYYIGNVDMSTIPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLL 123

Query: 143 ILGVAFYLQFYGKKQ 156
           I+GVAFYLQFYGKKQ
Sbjct: 124 IMGVAFYLQFYGKKQ 138

[Close]

BLAST of Sgr025443 vs. ExPASy Swiss-Prot
Match: Q9ZWT2 (Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 3.5e-37
Identity = 72/131 (54.96%), Postives = 96/131 (73.28%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           D KVF   EV++H+  +DCWI+I GKVYDVT FL++HPGGDEV+L +  KDATDDFE+VG
Sbjct: 4   DGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLIL 142
           HS  A   +++YYVG +D +TVP    + PP S  A     +SS  +I+LLQFL+PLLIL
Sbjct: 64  HSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPLLIL 123

Query: 143 GVAFYLQFYGK 154
           G+AF +++Y K
Sbjct: 124 GLAFGIRYYTK 134

[Close]

BLAST of Sgr025443 vs. ExPASy Swiss-Prot
Match: P49098 (Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 1.3e-36
Identity = 72/135 (53.33%), Postives = 101/135 (74.81%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           + KVF   EV++HN+ +DCW++ISGKVYDVT FL++HPGGDEVLL A  KDATDDFE+VG
Sbjct: 4   ETKVFTLAEVSQHNNAKDCWLVISGKVYDVTKFLDDHPGGDEVLLSATGKDATDDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLIL 142
           HS +A   +++YYVG +D +T+P    Y PP + P      ++S  +++LLQFL+PL+IL
Sbjct: 64  HSSSARAMLDEYYVGDIDSATIPTKTKYTPP-NQPHYNQD-KTSEFVVKLLQFLVPLIIL 123

Query: 143 GVAFYLQFYGKKQQS 158
           GVAF ++FY K+  +
Sbjct: 124 GVAFGIRFYTKQSSA 136

[Close]

BLAST of Sgr025443 vs. ExPASy Swiss-Prot
Match: O48845 (Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.9e-35
Identity = 70/131 (53.44%), Postives = 96/131 (73.28%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           + K+F   EV++HN   DCWI+I+GKVY+VT FLE+HPGGD+VLL +  KDATDDFE+VG
Sbjct: 4   EAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLIL 142
           HS +A E ME+YYVG++D +T+PK + Y PP          ++S  +I+LLQFL+PL IL
Sbjct: 64  HSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN--QDKTSEFIIKLLQFLVPLAIL 123

Query: 143 GVAFYLQFYGK 154
           G+A  ++ Y K
Sbjct: 124 GLAVGIRIYTK 132

[Close]

BLAST of Sgr025443 vs. ExPASy Swiss-Prot
Match: P49099 (Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 2.5e-35
Identity = 70/130 (53.85%), Postives = 95/130 (73.08%), Query Frame = 0


Query: 25  KVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHS 84
           KVF   EV+ HN+ +DCW+IISGKVY+VT FLE+HPGG EVLL A  KDATDDFE++GHS
Sbjct: 6   KVFTLAEVSNHNNAKDCWLIISGKVYNVTKFLEDHPGGGEVLLSATGKDATDDFEDIGHS 65

Query: 85  LAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLILGV 144
            +A   +++YYVG +D ST+P  + Y PP          +++  +++LLQFL+PL+ILGV
Sbjct: 66  SSARAMLDEYYVGDIDSSTIPTKVKYTPPKQPHYN--QDKTTEFIVKLLQFLVPLIILGV 125

Query: 145 AFYLQFYGKK 155
           AF + FY K+
Sbjct: 126 AFGVHFYTKQ 133

[Close]

BLAST of Sgr025443 vs. ExPASy Swiss-Prot
Match: P40934 (Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 3.3e-35
Identity = 72/133 (54.14%), Postives = 99/133 (74.44%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           + KV  F+EV++HN  +DCW+IISGKVYDVTPF+++HPGGDEVLL +  KDAT+DFE+VG
Sbjct: 4   EKKVLGFEEVSQHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLIL 142
           HS  A + MEKYY+G++D STVP    Y  P   PA     ++   +I++LQFL+P+LIL
Sbjct: 64  HSDTARDMMEKYYIGEIDSSTVPATRTYVAPV-QPAYNQD-KTPEFMIKILQFLVPILIL 123

Query: 143 GVAFYLQFYGKKQ 156
           G+A  ++ Y KK+
Sbjct: 124 GLALVVRQYTKKE 134

[Close]

BLAST of Sgr025443 vs. ExPASy TrEMBL
Match: A0A6J1D2G0 (cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111016385 PE=3 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 2.4e-60
Identity = 115/134 (85.82%), Postives = 126/134 (94.03%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVF FDEVAKHNH RDCW+IISGKVYDVTPFLEEHPGGDEVL+LA+EKDATDDFENVG
Sbjct: 4   DPKVFAFDEVAKHNHQRDCWLIISGKVYDVTPFLEEHPGGDEVLILASEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATT-PSAQSSGSLIRLLQFLIPLLI 142
           HSL+AI+QME+YYVG VDMSTVPKPIDYRPPAS  ATT  SAQSSGS ++LLQFLIPLL+
Sbjct: 64  HSLSAIDQMEQYYVGNVDMSTVPKPIDYRPPASQSATTLASAQSSGSSLKLLQFLIPLLL 123

Query: 143 LGVAFYLQFYGKKQ 156
           +GVAFY+QFYGKKQ
Sbjct: 124 IGVAFYMQFYGKKQ 137

[Close]

BLAST of Sgr025443 vs. ExPASy TrEMBL
Match: A0A6J1IEZ4 (cytochrome B5-like OS=Cucurbita maxima OX=3661 GN=LOC111473609 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.3e-58
Identity = 116/135 (85.93%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVT FLEEHPGGDEVLLLAAEKDATDDFENVG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASH--PATTPSAQSSGSLIRLLQFLIPLL 142
           HSL+AIEQMEKYY+G VDMSTVPKPIDYR  AS    ATT SA+SSGSL+ LLQ L+PLL
Sbjct: 64  HSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLL 123

Query: 143 ILGVAFYLQFYGKKQ 156
           I+GVAFYLQFYGKKQ
Sbjct: 124 IMGVAFYLQFYGKKQ 138

[Close]

BLAST of Sgr025443 vs. ExPASy TrEMBL
Match: A0A6J1ET11 (cytochrome B5-like OS=Cucurbita moschata OX=3662 GN=LOC111437191 PE=3 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.3e-58
Identity = 116/135 (85.93%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVT FLEEHPGGDEVLLLAAEKDATDDFENVG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASH--PATTPSAQSSGSLIRLLQFLIPLL 142
           HSL+AIEQMEKYY+G VDMSTVPKPIDYR  AS    ATT SA+SSGSL+ LLQ L+PLL
Sbjct: 64  HSLSAIEQMEKYYIGNVDMSTVPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLL 123

Query: 143 ILGVAFYLQFYGKKQ 156
           I+GVAFYLQFYGKKQ
Sbjct: 124 IMGVAFYLQFYGKKQ 138

[Close]

BLAST of Sgr025443 vs. ExPASy TrEMBL
Match: A0A6J1IDQ5 (cytochrome B5-like OS=Cucurbita maxima OX=3661 GN=LOC111474411 PE=3 SV=1)

HSP 1 Score: 235.3 bits (599), Expect = 1.7e-58
Identity = 115/135 (85.19%), Postives = 123/135 (91.11%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPKVFVFDEVAKHNH RDCW+IISGKVYDVT FLEEHPGGDEVLLLAAEKDATDDFENVG
Sbjct: 4   DPKVFVFDEVAKHNHQRDCWLIISGKVYDVTSFLEEHPGGDEVLLLAAEKDATDDFENVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASH--PATTPSAQSSGSLIRLLQFLIPLL 142
           HSL+AIEQMEKYY+G VDMST+PKPIDYR  AS    ATT SA+SSGSL+ LLQ L+PLL
Sbjct: 64  HSLSAIEQMEKYYIGNVDMSTIPKPIDYRLAASQSVKATTSSAESSGSLLTLLQILVPLL 123

Query: 143 ILGVAFYLQFYGKKQ 156
           I+GVAFYLQFYGKKQ
Sbjct: 124 IMGVAFYLQFYGKKQ 138

[Close]

BLAST of Sgr025443 vs. ExPASy TrEMBL
Match: A0A0A0L720 (Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119260 PE=3 SV=1)

HSP 1 Score: 218.8 bits (556), Expect = 1.6e-53
Identity = 105/142 (73.94%), Postives = 119/142 (83.80%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           DPK+FVFDEVAKHNH  DCW+IISGKVYDVTPFLE+HPGGDEVLLLA EKDAT+DFE V 
Sbjct: 4   DPKLFVFDEVAKHNHQADCWLIISGKVYDVTPFLEDHPGGDEVLLLATEKDATEDFETVS 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPAT---------TPSAQSSGSLIRLL 142
           HSL A E+MEKYY+G +DMST+PKP D+RPPAS   +         T SAQSSGSLI++L
Sbjct: 64  HSLDATEEMEKYYIGNIDMSTIPKPADHRPPASKSESAATEAATEKTSSAQSSGSLIKVL 123

Query: 143 QFLIPLLILGVAFYLQFYGKKQ 156
           Q LIPLLI+GVAFYLQ+YGKKQ
Sbjct: 124 QVLIPLLIIGVAFYLQYYGKKQ 145

[Close]

BLAST of Sgr025443 vs. TAIR 10
Match: AT5G48810.1 (cytochrome B5 isoform D )

HSP 1 Score: 156.0 bits (393), Expect = 2.5e-38
Identity = 72/131 (54.96%), Postives = 96/131 (73.28%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           D KVF   EV++H+  +DCWI+I GKVYDVT FL++HPGGDEV+L +  KDATDDFE+VG
Sbjct: 4   DGKVFTLSEVSQHSSAKDCWIVIDGKVYDVTKFLDDHPGGDEVILTSTGKDATDDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLIL 142
           HS  A   +++YYVG +D +TVP    + PP S  A     +SS  +I+LLQFL+PLLIL
Sbjct: 64  HSSTAKAMLDEYYVGDIDTATVPVKAKFVPPTSTKAVATQDKSSDFVIKLLQFLVPLLIL 123

Query: 143 GVAFYLQFYGK 154
           G+AF +++Y K
Sbjct: 124 GLAFGIRYYTK 134

[Close]

BLAST of Sgr025443 vs. TAIR 10
Match: AT2G32720.1 (cytochrome B5 isoform B )

HSP 1 Score: 150.2 bits (378), Expect = 1.4e-36
Identity = 70/131 (53.44%), Postives = 96/131 (73.28%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           + K+F   EV++HN   DCWI+I+GKVY+VT FLE+HPGGD+VLL +  KDATDDFE+VG
Sbjct: 4   EAKIFTLSEVSEHNQAHDCWIVINGKVYNVTKFLEDHPGGDDVLLSSTGKDATDDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLIL 142
           HS +A E ME+YYVG++D +T+PK + Y PP          ++S  +I+LLQFL+PL IL
Sbjct: 64  HSESAREMMEQYYVGEIDPTTIPKKVKYTPPKQPHYN--QDKTSEFIIKLLQFLVPLAIL 123

Query: 143 GVAFYLQFYGK 154
           G+A  ++ Y K
Sbjct: 124 GLAVGIRIYTK 132

[Close]

BLAST of Sgr025443 vs. TAIR 10
Match: AT5G53560.1 (cytochrome B5 isoform E )

HSP 1 Score: 148.3 bits (373), Expect = 5.3e-36
Identity = 72/135 (53.33%), Postives = 99/135 (73.33%), Query Frame = 0


Query: 23  DPKVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVG 82
           D KV  F+EV+KHN  +DCW+IISGKVYDVTPF+++HPGGDEVLL +  KDAT+DFE+VG
Sbjct: 4   DRKVLSFEEVSKHNKTKDCWLIISGKVYDVTPFMDDHPGGDEVLLSSTGKDATNDFEDVG 63

Query: 83  HSLAAIEQMEKYYVGKVDMSTVPKPIDY---RPPASHPATTPSAQSSGSLIRLLQFLIPL 142
           HS  A + M+KY++G++D S+VP    Y   + PA +   TP       +I++LQFL+P+
Sbjct: 64  HSDTARDMMDKYFIGEIDSSSVPATRTYVAPQQPAYNQDKTPE-----FIIKILQFLVPI 123

Query: 143 LILGVAFYLQFYGKK 155
           LILG+A  ++ Y KK
Sbjct: 124 LILGLALVVRHYTKK 133

[Close]

BLAST of Sgr025443 vs. TAIR 10
Match: AT2G46650.1 (cytochrome B5 isoform C )

HSP 1 Score: 134.0 bits (336), Expect = 1.0e-31
Identity = 68/126 (53.97%), Postives = 82/126 (65.08%), Query Frame = 0


Query: 29  FDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHSLAAI 88
           F +VAKH    DCWI+I GKVYD++ F++EHPGGD VLL    KDA+ DFE+V HS  A 
Sbjct: 7   FHDVAKHKCKNDCWILIHGKVYDISTFMDEHPGGDNVLLAVTGKDASIDFEDVNHSKDAK 66

Query: 89  EQMEKYYVGKVDMSTVPKPIDYRPPASHPATTPSAQSSGSLIRLLQFLIPLLILGVAFYL 148
           E M+KY +G VD STVP    Y PP    +T        S  +LL +LIPLLILGVAF L
Sbjct: 67  ELMKKYCIGDVDQSTVPVTQQYIPPWEKESTAAETTKEESGKKLLIYLIPLLILGVAFAL 126

Query: 149 QFYGKK 155
           +FY  K
Sbjct: 127 RFYNNK 132

[Close]

BLAST of Sgr025443 vs. TAIR 10
Match: AT1G26340.1 (cytochrome B5 isoform A )

HSP 1 Score: 111.7 bits (278), Expect = 5.4e-25
Identity = 46/82 (56.10%), Postives = 64/82 (78.05%), Query Frame = 0


Query: 25  KVFVFDEVAKHNHLRDCWIIISGKVYDVTPFLEEHPGGDEVLLLAAEKDATDDFENVGHS 84
           K++  +E A HN   DCW++I GKVYDV+ +++EHPGGD+VLL  A KDATDDFE+ GHS
Sbjct: 6   KLYSMEEAATHNKQDDCWVVIDGKVYDVSSYMDEHPGGDDVLLAVAGKDATDDFEDAGHS 65

Query: 85  LAAIEQMEKYYVGKVDMSTVPK 107
             A E MEKY++G++D S++P+
Sbjct: 66  KDARELMEKYFIGELDESSLPE 87

The following BLAST results are available for this feature:

BLAST of Sgr025443 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147466.1	4.9e-60	85.82	cytochrome b5-like [Momordica charantia]	[more]
XP_023540248.1	3.2e-59	86.67	cytochrome b5-like [Cucurbita pepo subsp. pepo]	[more]
XP_022931217.1	2.7e-58	85.93	cytochrome B5-like [Cucurbita moschata] >XP_022974834.1 cytochrome B5-like [Cucu...	[more]
XP_038901262.1	2.7e-58	81.56	cytochrome b5-like [Benincasa hispida]	[more]
XP_022975276.1	3.5e-58	85.19	cytochrome B5-like [Cucurbita maxima]	[more]

BLAST of Sgr025443 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZWT2	3.5e-37	54.96	Cytochrome B5 isoform D OS=Arabidopsis thaliana OX=3702 GN=CYTB5-D PE=1 SV=1	[more]
P49098	1.3e-36	53.33	Cytochrome b5 OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
O48845	1.9e-35	53.44	Cytochrome b5 isoform B OS=Arabidopsis thaliana OX=3702 GN=CYTB5-B PE=1 SV=1	[more]
P49099	2.5e-35	53.85	Cytochrome b5, seed isoform OS=Nicotiana tabacum OX=4097 PE=2 SV=1	[more]
P40934	3.3e-35	54.14	Cytochrome b5 OS=Brassica oleracea var. botrytis OX=3715 GN=CYB5 PE=1 SV=1	[more]

BLAST of Sgr025443 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2G0	2.4e-60	85.82	cytochrome b5-like OS=Momordica charantia OX=3673 GN=LOC111016385 PE=3 SV=1	[more]
A0A6J1IEZ4	1.3e-58	85.93	cytochrome B5-like OS=Cucurbita maxima OX=3661 GN=LOC111473609 PE=3 SV=1	[more]
A0A6J1ET11	1.3e-58	85.93	cytochrome B5-like OS=Cucurbita moschata OX=3662 GN=LOC111437191 PE=3 SV=1	[more]
A0A6J1IDQ5	1.7e-58	85.19	cytochrome B5-like OS=Cucurbita maxima OX=3661 GN=LOC111474411 PE=3 SV=1	[more]
A0A0A0L720	1.6e-53	73.94	Cytochrome b5 heme-binding domain-containing protein OS=Cucumis sativus OX=3659 ...	[more]

BLAST of Sgr025443 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G48810.1	2.5e-38	54.96	cytochrome B5 isoform D	[more]
AT2G32720.1	1.4e-36	53.44	cytochrome B5 isoform B	[more]
AT5G53560.1	5.3e-36	53.33	cytochrome B5 isoform E	[more]
AT2G46650.1	1.0e-31	53.97	cytochrome B5 isoform C	[more]
AT1G26340.1	5.4e-25	56.10	cytochrome B5 isoform A	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001199	Cytochrome b5-like heme/steroid binding domain	PRINTS	PR00363	CYTOCHROMEB5	coord: 87..99 score: 30.3 coord: 74..81 score: 43.94 coord: 59..73 score: 52.32 coord: 49..59 score: 66.53
IPR001199	Cytochrome b5-like heme/steroid binding domain	SMART	SM01117	Cyt_b5_2	coord: 27..100 e-value: 2.6E-28 score: 110.1
IPR001199	Cytochrome b5-like heme/steroid binding domain	PFAM	PF00173	Cyt-b5	coord: 30..99 e-value: 1.6E-23 score: 82.6
IPR001199	Cytochrome b5-like heme/steroid binding domain	PROSITE	PS50255	CYTOCHROME_B5_2	coord: 24..100 score: 27.540628
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	GENE3D	3.10.120.10		coord: 22..104 e-value: 4.4E-32 score: 112.0
IPR036400	Cytochrome b5-like heme/steroid binding domain superfamily	SUPERFAMILY	55856	Cytochrome b5-like heme/steroid binding domain	coord: 21..113
None	No IPR available	PANTHER	PTHR19359	CYTOCHROME B5	coord: 23..153
None	No IPR available	PANTHER	PTHR19359:SF81	SUBFAMILY NOT NAMED	coord: 23..153
IPR018506	Cytochrome b5, heme-binding site	PROSITE	PS00191	CYTOCHROME_B5_1	coord: 55..62

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr025443.1	Sgr025443.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0001763	morphogenesis of a branching structure
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0043231	intracellular membrane-bounded organelle
molecular_function	GO:0020037	heme binding
molecular_function	GO:0046872	metal ion binding