Sgr025435 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025435
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionProtein kinase domain-containing protein
Locationtig00006406: 1317787 .. 1320048 (+)
RNA-Seq ExpressionSgr025435
SyntenySgr025435
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTACAAGCATTTGACGAAGTCGATGGGATTGAAGTTGCCTGGAACCAAGTTAGGATTGATGGTTTCTTGCAGTCACCCGAGGATTTAGAAAAGTTATACTCCGAAGTACATCTACTTAAATCATTAAAACATGAGAATATTATCAAGTTCTATAATTCTTGGGTGGATGATAAGAAGAAAACAGTCAACATGATCACTGAGCTCTTCACATCTGGGAGTTTGAGGCAGTATGTTTTCAGTTTGAGGTTACGTAATTAGTCTTTAATGGTGTGACTTAATTTTCTGTTTAGTTATCATGTGCCTTTTGGCTATCAGATACCGTAAAAAGCATAAACATGTTGATATGAAGGCCATAAAGAACTGGGCACGACAGGTTCTCCGAGGATTGGTCTATCTTCACAGCCACAATCCTCCTATTATTCACAGAGATTTGAAAGGTGATAATATTTTTATTAATGGAAATCATGGAGAAGTTAAAATTGGAGATCTTGGATTAGCAATTGTTATGCAGCAGCCTACCGCACGAAGTGTAATTGGTATGTTAACAGTTAATGTATCACTTGTTGTCTTCGTATAACTAAAATACTTAAGGTACAAAGTACTGAAATTCTTCCTTCTTTATGCAAGGAACTCCTGAGTTTATGGCTCCTGAGCTTTATGAAGAGGAGTACAATGAGCTTGTTGATGTATATTCTTTTGGGATGTGCATGCTGGAGATGGTTACCTTCGAGTATCCATATAGTGAATGTAAAAATCCAGCTCAGATCTATAAGAAAGTTACATCTGTAAGCGAAACAATCGTCACTTCATCATCATGCAACATGTTAACTGTTCTTGCTGACCATTATTCCAATACTGACCTTCTTCTTAATCAGGGTATTAAACCTGCTTCCCTTGCTAAGGTGAGTGATCCACAGACCATGGAGTTTATCAATAAATGTTTGGTTCCAGTGCACGAGAGATTGTCTGCAAAAGAGCTTCTTCAAGATTCATTCCTTCAAGTTGAGAACCCAAAGGAGGCAACACGTGATCCCCTACAGTTACCTAACCATGTTCCCAGATCAATAAATTTGCCAAAGAGTGGGCCTATTTCCATGGACATAGACATTGACCATAGACAACACTCTTTAAGCACTTGTGCAGGAAGCAATGGTGGGAGTCCACGCTTTCCAGTTGTGGAATTCCAGACGATGAATAAGAACAACGAGTTTAGGTTACGTGGAAATAAGAATGATGACAACTCAGTGGCATTAACCCTGCGTATTGCAGACTCAAGTGGTAAGCTTAACAGTGAGACTCTCACCTTTTCCCTTCTGCTAGTGATTGATTTTTGTTCCTTTCTCATTATGAACATGAAGTTCTCTTAAACTTGCAGGTCGAGTGAGGAATATACATTTTACGTTTATCTCGATTCTGATACTGCACTATCAGTGGCAGCTGAAATGGCTGAACAATTGGAGTTGGTAGATCATGATGTGGATTTCATAGCTGAATTTATTGATTTTTTGATCGCAAAACTTATACCTGATTGGAAGCCTTTGTCTGATTATTCCTCAAGTGGAGCAATAAGTCTTTGTGGCGGCTCCCATATCCTTAAAAACACCAAATCATCTACGCATCTCCGTGGGGTTCAATGTTAACTGGGGTTCAAGATGGGTTGTTGGTCGAACAAGACACTTCCTCTGGGTTAGTGAGTACTCAAAAAGACTGTCTGCAATCTGAGGAAGTTGGCTGGGCTGATGATATTCCCAGTAGTATTATTTTTGATACTGGTTGCCCTTCTTCTCCTAGTTTGGCTAATTTTGAGGACCTGCATTCGCAAGCTTCTTTCGCATCGGAGATATTGGTCGAAGATAGTTCTACCAAAAGTGTAAGGTGATAGATTGTTCCAATGTTGATGGAAGCTCAAAAGGCTCGAGCTGGTCGATTGCAGAACTAGAGCTCCATGGTTCATCATATGTTGATGACAAATTGCAAAGAAATGCTAGTGATGTTGGAATTTTCACTCCAATGGATTATTTTGTTAAGAACTCAGTCATGTCATTGCCAGCTCCAAGTGAAGTATCTAATGTGATGAGCCTGACGAGCAGCTGTTCTTCGCTATCCTTAGCGGACAAGGATCTGGATGCCGAACTCAAGTTGGAACTCAATGCGATCGAGACACATTATCAGCAGTTGTTCGAAGAGCTTTCTCGGATGAGAGAGGAAGCACTTGAGGCCACCCGAAAGAGATGGATAGCAAAGAAGAAACTTATTCATTGA

mRNA sequence

TTACAAGCATTTGACGAAGTCGATGGGATTGAAGTTGCCTGGAACCAAGTTAGGATTGATGGTTTCTTGCAGTCACCCGAGGATTTAGAAAAGTTATACTCCGAAGTACATCTACTTAAATCATTAAAACATGAGAATATTATCAAGTTCTATAATTCTTGGGTGGATGATAAGAAGAAAACAGTCAACATGATCACTGAGCTCTTCACATCTGGGAGTTTGAGGCAATACCGTAAAAAGCATAAACATGTTGATATGAAGGCCATAAAGAACTGGGCACGACAGGTTCTCCGAGGATTGGTCTATCTTCACAGCCACAATCCTCCTATTATTCACAGAGATTTGAAAGGTGATAATATTTTTATTAATGGAAATCATGGAGAAGTTAAAATTGGAGATCTTGGATTAGCAATTGTTATGCAGCAGCCTACCGCACGAAGTGTAATTGGAACTCCTGAGTTTATGGCTCCTGAGCTTTATGAAGAGGAGTACAATGAGCTTGTTGATGTATATTCTTTTGGGATGTGCATGCTGGAGATGGTTACCTTCGAGTATCCATATAGTGAATGTAAAAATCCAGCTCAGATCTATAAGAAAGTTACATCTGTAAGCGAAACAATCGTCACTTCATCATCATGCAACATGTTAACTGTTCTTGCTGACCATTATTCCAATACTGACCTTCTTCTTAATCAGGGTATTAAACCTGCTTCCCTTGCTAAGGTGAGTGATCCACAGACCATGGAGTTTATCAATAAATGTTTGGTTCCAGTGCACGAGAGATTGTCTGCAAAAGAGCTTCTTCAAGATTCATTCCTTCAAGTTGAGAACCCAAAGGAGGCAACACGTGATCCCCTACAGTTACCTAACCATGTTCCCAGATCAATAAATTTGCCAAAGAGTGGGCCTATTTCCATGGACATAGACATTGACCATAGACAACACTCTTTAAGCACTTGTGCAGGAAGCAATGGTGGGAGTCCACGCTTTCCAGTTGTGGAATTCCAGACGATGAATAAGAACAACGAGTTTAGGTTACGTGGAAATAAGAATGATGACAACTCAGTGGCATTAACCCTGCGTATTGCAGACTCAAGTGGTAAGCTTAACATGGCAGCTGAAATGGCTGAACAATTGGAGTTGGTAGATCATGATGTGGATTTCATAGCTGAATTTATTGATTTTTTGATCGCAAAACTTATACCTGATTGGAAGCCTTTGTCTGATTATTCCTCAAGTGGAGCAATAAATGGGTTGTTGGTCGAACAAGACACTTCCTCTGGGTTAGTGAGTACTCAAAAAGACTGTCTGCAATCTGAGGAAGTTGGCTGGGCTGATGATATTCCCAGTAGTATTATTTTTGATACTGGTTGCCCTTCTTCTCCTAGTTTGGCTAATTTTGAGGACCTGCATTCGCAAGCTTCTTTCGCATCGGAGATATTGGTCGAAGATAGTTCTACCAAAAGTGTAAGCTGGTCGATTGCAGAACTAGAGCTCCATGGTTCATCATATGTTGATGACAAATTGCAAAGAAATGCTAGTGATGTTGGAATTTTCACTCCAATGGATTATTTTGTTAAGAACTCAGTCATGTCATTGCCAGCTCCAAGTGAAGTATCTAATGTGATGAGCCTGACGAGCAGCTGTTCTTCGCTATCCTTAGCGGACAAGGATCTGGATGCCGAACTCAAGTTGGAACTCAATGCGATCGAGACACATTATCAGCAGTTGTTCGAAGAGCTTTCTCGGATGAGAGAGGAAGCACTTGAGGCCACCCGAAAGAGATGGATAGCAAAGAAGAAACTTATTCATTGA

Coding sequence (CDS)

TTACAAGCATTTGACGAAGTCGATGGGATTGAAGTTGCCTGGAACCAAGTTAGGATTGATGGTTTCTTGCAGTCACCCGAGGATTTAGAAAAGTTATACTCCGAAGTACATCTACTTAAATCATTAAAACATGAGAATATTATCAAGTTCTATAATTCTTGGGTGGATGATAAGAAGAAAACAGTCAACATGATCACTGAGCTCTTCACATCTGGGAGTTTGAGGCAATACCGTAAAAAGCATAAACATGTTGATATGAAGGCCATAAAGAACTGGGCACGACAGGTTCTCCGAGGATTGGTCTATCTTCACAGCCACAATCCTCCTATTATTCACAGAGATTTGAAAGGTGATAATATTTTTATTAATGGAAATCATGGAGAAGTTAAAATTGGAGATCTTGGATTAGCAATTGTTATGCAGCAGCCTACCGCACGAAGTGTAATTGGAACTCCTGAGTTTATGGCTCCTGAGCTTTATGAAGAGGAGTACAATGAGCTTGTTGATGTATATTCTTTTGGGATGTGCATGCTGGAGATGGTTACCTTCGAGTATCCATATAGTGAATGTAAAAATCCAGCTCAGATCTATAAGAAAGTTACATCTGTAAGCGAAACAATCGTCACTTCATCATCATGCAACATGTTAACTGTTCTTGCTGACCATTATTCCAATACTGACCTTCTTCTTAATCAGGGTATTAAACCTGCTTCCCTTGCTAAGGTGAGTGATCCACAGACCATGGAGTTTATCAATAAATGTTTGGTTCCAGTGCACGAGAGATTGTCTGCAAAAGAGCTTCTTCAAGATTCATTCCTTCAAGTTGAGAACCCAAAGGAGGCAACACGTGATCCCCTACAGTTACCTAACCATGTTCCCAGATCAATAAATTTGCCAAAGAGTGGGCCTATTTCCATGGACATAGACATTGACCATAGACAACACTCTTTAAGCACTTGTGCAGGAAGCAATGGTGGGAGTCCACGCTTTCCAGTTGTGGAATTCCAGACGATGAATAAGAACAACGAGTTTAGGTTACGTGGAAATAAGAATGATGACAACTCAGTGGCATTAACCCTGCGTATTGCAGACTCAAGTGGTAAGCTTAACATGGCAGCTGAAATGGCTGAACAATTGGAGTTGGTAGATCATGATGTGGATTTCATAGCTGAATTTATTGATTTTTTGATCGCAAAACTTATACCTGATTGGAAGCCTTTGTCTGATTATTCCTCAAGTGGAGCAATAAATGGGTTGTTGGTCGAACAAGACACTTCCTCTGGGTTAGTGAGTACTCAAAAAGACTGTCTGCAATCTGAGGAAGTTGGCTGGGCTGATGATATTCCCAGTAGTATTATTTTTGATACTGGTTGCCCTTCTTCTCCTAGTTTGGCTAATTTTGAGGACCTGCATTCGCAAGCTTCTTTCGCATCGGAGATATTGGTCGAAGATAGTTCTACCAAAAGTGTAAGCTGGTCGATTGCAGAACTAGAGCTCCATGGTTCATCATATGTTGATGACAAATTGCAAAGAAATGCTAGTGATGTTGGAATTTTCACTCCAATGGATTATTTTGTTAAGAACTCAGTCATGTCATTGCCAGCTCCAAGTGAAGTATCTAATGTGATGAGCCTGACGAGCAGCTGTTCTTCGCTATCCTTAGCGGACAAGGATCTGGATGCCGAACTCAAGTTGGAACTCAATGCGATCGAGACACATTATCAGCAGTTGTTCGAAGAGCTTTCTCGGATGAGAGAGGAAGCACTTGAGGCCACCCGAAAGAGATGGATAGCAAAGAAGAAACTTATTCATTGA

Protein sequence

LQAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKTVNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIFINGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMVTFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAKVSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKSGPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLRIADSSGKLNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKPLSDYSSSGAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLIH
Homology
BLAST of Sgr025435 vs. NCBI nr
Match: XP_038893325.1 (probable serine/threonine-protein kinase WNK7 isoform X1 [Benincasa hispida])

HSP 1 Score: 954.1 bits (2465), Expect = 5.7e-274
Identity = 509/661 (77.00%), Postives = 543/661 (82.15%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQV+IDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT
Sbjct: 33  KAFDEVDGIEVAWNQVKIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 92

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 93  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHDPPIIHRDLKGDNIF 152

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 153 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 212

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPY ECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 213 TFEYPYCECKNPAQIFRKVTS------------------------------GIKPASLAK 272

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDPQTMEFINKCLVP HERLSAKELL+DSFLQVENPKE+TR+PLQLPN V RS+NLPKS
Sbjct: 273 VSDPQTMEFINKCLVPAHERLSAKELLKDSFLQVENPKESTRNPLQLPNQVLRSLNLPKS 332

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDIDH+QHSLST A SN GSPR PVVEFQTMNKNNEFRLRGNKNDDNSVALT+R
Sbjct: 333 GPISMDIDIDHKQHSLSTHAESNNGSPRCPVVEFQTMNKNNEFRLRGNKNDDNSVALTMR 392

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IADS+G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP WKP
Sbjct: 393 IADSNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPGWKP 452

Query: 422 LSDYSSS---------------------------GAINGLLVEQDTSSGLV-STQKDCLQ 481
           LS YSS+                             I+  LVEQD SSGLV STQKDCLQ
Sbjct: 453 LSVYSSNVESNLCDGPPILKSDKSSIGSPWGSMFTGIHDGLVEQDISSGLVCSTQKDCLQ 512

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SE   WADD+P S IFDT CPSSPSLAN EDL+S ASFASE+LVED STKS         
Sbjct: 513 SERDDWADDVPGSNIFDT-CPSSPSLANLEDLNSHASFASELLVEDCSTKSAKVYDCSNI 572

Query: 542 -------SWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVM 601
                  +WSIAELE HGSSYV+DKLQ N SDVG+FTPMDYF+KNSV+SLPAPSE S+V+
Sbjct: 573 DGSSKGSNWSIAELEPHGSSYVEDKLQINVSDVGMFTPMDYFLKNSVVSLPAPSEASHVI 632

Query: 602 SLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLI 605
           SLTSSCSSLSLADKDL+AELK+E+NAIETHY+QLF+ELSRMREEALEATRKRWIAKKKLI
Sbjct: 633 SLTSSCSSLSLADKDLNAELKMEINAIETHYRQLFDELSRMREEALEATRKRWIAKKKLI 662

BLAST of Sgr025435 vs. NCBI nr
Match: XP_004134295.1 (probable serine/threonine-protein kinase WNK7 isoform X1 [Cucumis sativus] >KAE8650223.1 hypothetical protein Csa_010310 [Cucumis sativus])

HSP 1 Score: 940.6 bits (2430), Expect = 6.5e-270
Identity = 503/662 (75.98%), Postives = 537/662 (81.12%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT
Sbjct: 33  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 92

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 93  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 152

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 153 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 212

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 213 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 272

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELL+DSFLQVENPKE+ R+PLQL N V +SINLPKS
Sbjct: 273 VSDPRTMEFINKCLVPVHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKS 332

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDID + HSLST A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 333 GPISMDIDIDQKIHSLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 392

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IADS+G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP+WKP
Sbjct: 393 IADSNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKP 452

Query: 422 LSDYSSSGAINGL---------------------------LVEQDTSSGL-VSTQKDCLQ 481
           LS YSS+G ++                             LV QD SSGL   TQKDCLQ
Sbjct: 453 LSVYSSNGELSLFSAPPFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQ 512

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SEE GW  DI +  IFDT CPSSPSLANFEDL+S ASFA E+LV+D STKS         
Sbjct: 513 SEEDGWTTDISAGHIFDT-CPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNI 572

Query: 542 -------SWSIAELELHGSSY-VDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNV 601
                  SWSIAELE HGSSY V+DK QRN  DVGIFTPMDYF KNSV+S+PAPSE SNV
Sbjct: 573 DGSSKGSSWSIAELEHHGSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNV 632

Query: 602 MSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKL 605
           MSLTSSCSSLSL DKDLDAELK+E++AIETHY+QLF+ELSRMREEALEATR+RWIAKKKL
Sbjct: 633 MSLTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 663

BLAST of Sgr025435 vs. NCBI nr
Match: XP_011650666.1 (probable serine/threonine-protein kinase WNK7 isoform X2 [Cucumis sativus])

HSP 1 Score: 940.6 bits (2430), Expect = 6.5e-270
Identity = 503/662 (75.98%), Postives = 537/662 (81.12%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT
Sbjct: 15  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 74

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 75  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 134

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 135 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 194

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 195 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 254

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELL+DSFLQVENPKE+ R+PLQL N V +SINLPKS
Sbjct: 255 VSDPRTMEFINKCLVPVHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKS 314

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDID + HSLST A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 315 GPISMDIDIDQKIHSLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 374

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IADS+G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP+WKP
Sbjct: 375 IADSNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKP 434

Query: 422 LSDYSSSGAINGL---------------------------LVEQDTSSGL-VSTQKDCLQ 481
           LS YSS+G ++                             LV QD SSGL   TQKDCLQ
Sbjct: 435 LSVYSSNGELSLFSAPPFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQ 494

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SEE GW  DI +  IFDT CPSSPSLANFEDL+S ASFA E+LV+D STKS         
Sbjct: 495 SEEDGWTTDISAGHIFDT-CPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNI 554

Query: 542 -------SWSIAELELHGSSY-VDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNV 601
                  SWSIAELE HGSSY V+DK QRN  DVGIFTPMDYF KNSV+S+PAPSE SNV
Sbjct: 555 DGSSKGSSWSIAELEHHGSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNV 614

Query: 602 MSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKL 605
           MSLTSSCSSLSL DKDLDAELK+E++AIETHY+QLF+ELSRMREEALEATR+RWIAKKKL
Sbjct: 615 MSLTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 645

BLAST of Sgr025435 vs. NCBI nr
Match: XP_008437860.1 (PREDICTED: probable serine/threonine-protein kinase WNK7 [Cucumis melo])

HSP 1 Score: 934.9 bits (2415), Expect = 3.5e-268
Identity = 501/661 (75.79%), Postives = 532/661 (80.48%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 
Sbjct: 33  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKN 92

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 93  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 152

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 153 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 212

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 213 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 272

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELLQDSFLQVENPKE+ R+PLQL N V RSINLPKS
Sbjct: 273 VSDPRTMEFINKCLVPVHERLSAKELLQDSFLQVENPKESARNPLQLSNQVSRSINLPKS 332

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDIDH+ HSLS  A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 333 GPISMDIDIDHKLHSLSNYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 392

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD +G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP WKP
Sbjct: 393 IADPNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPGWKP 452

Query: 422 LSDYSSSG---------------------------AINGLLVEQDTSSGLV-STQKDCLQ 481
           LS YSS+G                             +  LV QDTSSG+V  TQ D LQ
Sbjct: 453 LSVYSSNGESSLCSAPPVLKSAKSSVRSPWGSMLTGSHDGLVAQDTSSGMVCGTQTDSLQ 512

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SE  GW  DI    IFDT CPSSPSLANFED +S ASFA E+LV+D STKS         
Sbjct: 513 SERNGWTGDISGGHIFDT-CPSSPSLANFEDSNSHASFALELLVDDCSTKSAKVFDCSNI 572

Query: 542 -------SWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVM 601
                  SWSIAELE  GSSYV+DKLQRN S VGIFTP+DYFVKNSV+S+PAPSE SNVM
Sbjct: 573 DGSSKGSSWSIAELEHQGSSYVEDKLQRNVSGVGIFTPLDYFVKNSVVSMPAPSEASNVM 632

Query: 602 SLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLI 605
           SLTSSCSSLSLADKDLDAELK+E++AIETHY+QLF+ELSRMREEALEATR+RWIAKKKLI
Sbjct: 633 SLTSSCSSLSLADKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLI 662

BLAST of Sgr025435 vs. NCBI nr
Match: KAA0048870.1 (putative serine/threonine-protein kinase WNK7 [Cucumis melo var. makuwa])

HSP 1 Score: 934.9 bits (2415), Expect = 3.5e-268
Identity = 500/661 (75.64%), Postives = 532/661 (80.48%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 
Sbjct: 99  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKN 158

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 159 VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 218

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 219 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 278

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 279 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 338

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELLQDSFLQVENPKE+ R+PLQL N VPRSINLPKS
Sbjct: 339 VSDPRTMEFINKCLVPVHERLSAKELLQDSFLQVENPKESARNPLQLSNQVPRSINLPKS 398

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDIDH+ HSLS  A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 399 GPISMDIDIDHKLHSLSNYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 458

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD +G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP WKP
Sbjct: 459 IADPNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPGWKP 518

Query: 422 LSDYSSSG---------------------------AINGLLVEQDTSSGLV-STQKDCLQ 481
           LS YSS+G                             +  LV QDTSSG+V  TQ D LQ
Sbjct: 519 LSVYSSNGESSLCSAPPVLKSAKSSMRSPWGSMLTGSHDGLVAQDTSSGMVCGTQTDSLQ 578

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SE  GW DDI    IFDT CPSSPSLANFED +S ASFA E+LV+D STKS         
Sbjct: 579 SERNGWTDDISGGHIFDT-CPSSPSLANFEDSNSHASFALELLVDDCSTKSAKVFDCSNI 638

Query: 542 -------SWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVM 601
                  SWS+AELE  GSSYV+DKLQRN S VGIFTP+DYF KNSV+S+PAPSE SNVM
Sbjct: 639 DGSSKGSSWSVAELEHQGSSYVEDKLQRNVSGVGIFTPLDYFGKNSVVSMPAPSEASNVM 698

Query: 602 SLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLI 605
           SLTSSCSSLSLADKDLDAELK+E++AI THY+QLF+ELSRMREEALEATR+RWIAKKKLI
Sbjct: 699 SLTSSCSSLSLADKDLDAELKMEIDAIGTHYRQLFDELSRMREEALEATRRRWIAKKKLI 728

BLAST of Sgr025435 vs. ExPASy Swiss-Prot
Match: Q6EU49 (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK4 PE=2 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 7.5e-136
Identity = 302/628 (48.09%), Postives = 380/628 (60.51%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEV+GIEVAW+QV ID  +QSP++LE+LYSEVHLLKSLKHEN++KFYN WVDD+KKT
Sbjct: 41  KAFDEVEGIEVAWSQVEIDEVMQSPDNLERLYSEVHLLKSLKHENVMKFYNYWVDDQKKT 100

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           +N+ITELFTSGSLRQYR+KH  VD+KAIKNWARQVLRGL YLH+H PPIIHRDLK DNIF
Sbjct: 101 INVITELFTSGSLRQYRQKHPRVDLKAIKNWARQVLRGLDYLHTHQPPIIHRDLKCDNIF 160

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           +NGNHGEVKIGDLGLA VM  P A+SVIGTPEFMAPELY+E Y+ELVD+YSFGMCMLEM 
Sbjct: 161 VNGNHGEVKIGDLGLATVMLTPRAKSVIGTPEFMAPELYDENYDELVDIYSFGMCMLEMF 220

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           T EYPYSEC N AQI+KKV                              ++G+KPA+LAK
Sbjct: 221 TLEYPYSECTNAAQIFKKV------------------------------SKGVKPAALAK 280

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPK--EATRDPLQLPNHVPRSINLP 301
           +++ Q  +FI+KCLVP  ERLSAKELLQD FL  +N      T+ P  LP  V  S+   
Sbjct: 281 ITNIQAKQFIDKCLVPASERLSAKELLQDPFLCSDNSSVLVGTKFPSSLPKSVDVSLE-- 340

Query: 302 KSGPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALT 361
               + MD+D +      STC  ++ G P   V+EF   NKN E +L G K DDNSV+L 
Sbjct: 341 ---ALHMDVDTNESM-CTSTCKRNDLGGPHRSVLEFTRTNKNTELKLTGEKLDDNSVSLV 400

Query: 362 LRIADSSG---------------KLNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDW 421
           LRIAD  G                +++AAEM EQLEL D DV FIA+FID LI  L+P  
Sbjct: 401 LRIADLCGHARNIHFLFYLDSDTAMSVAAEMVEQLELADCDVTFIADFIDLLIVNLVPGQ 460

Query: 422 KPLSD-------YSSSGAINGLLVEQDTSSGLVSTQ---KDCLQSEEVGWADDIPSSIIF 481
           + ++D        S  G    ++  Q   S L       +  + S+E   A+  PS  I 
Sbjct: 461 QLMNDAVMSTSSESKMGESEHVITSQQHPSELTHDYVLVEGMMHSKE---ANASPSDYI- 520

Query: 482 DTGCPSSPSLANFEDLHS-QASFASEILVE-DSSTKSVSWSIAELELHGSSYVDDKLQRN 541
                   SL N  +L    +S  S+I V+ D S+KS+S    E  +     ++    + 
Sbjct: 521 -------DSLLNATNLGGPNSSEGSDISVQLDGSSKSLS----EYGVDEYRTLECGAYKG 580

Query: 542 ASDVGIFTPMDYFVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAIET 601
              +G   P+     N   ++    + S+   L    S     ++D+   L  EL  IE 
Sbjct: 581 TDKLGCRHPLSNGSSN--FAIFQMDQASHHSELVIGASVSITENRDV---LNGELGLIEA 612

BLAST of Sgr025435 vs. ExPASy Swiss-Prot
Match: Q8LST2 (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN=WNK7 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 7.0e-126
Identity = 281/630 (44.60%), Postives = 352/630 (55.87%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           + FDEVDGIEVAWNQVRID  LQSP+ LE+LYSEV LLKSLKH+NII+FYNSW+DDK KT
Sbjct: 44  KGFDEVDGIEVAWNQVRIDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKT 103

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VN+ITELFTSGSLRQYRKKH+ V+MKA+K WARQ+L GL YLHS +PPIIHRD+K DNIF
Sbjct: 104 VNIITELFTSGSLRQYRKKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIF 163

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEMV
Sbjct: 164 INGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMV 223

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPY EC+N AQIYKKV+S                              GIKPASL+K
Sbjct: 224 TFEYPYCECRNSAQIYKKVSS------------------------------GIKPASLSK 283

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DP+ M+FI KCL+P  ERLSA+ELL DSFL V        +PL LP+     I +PK 
Sbjct: 284 VKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNG--LVMNNPLPLPD-----IVMPKE 343

Query: 302 GPI----------------SMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFR 361
           G                  +M +++D   +     + +N G+     +E +   + N F 
Sbjct: 344 GSFGERCLMSEGPPNARNRTMSMNLDEDNNLPIVISSNNSGT---NCIEVRRAKRGNFFV 403

Query: 362 LRGNKNDDNSVALTLRIADSSGKL---------------NMAAEMAEQLELVDHDVDFIA 421
           L+G +ND+NSV+L LRI D +G++               N+++EM EQLEL D +V FIA
Sbjct: 404 LKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVKFIA 463

Query: 422 EFIDFLIAKLIPDWKPLSDYSSSGAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPS 481
           E ID L+  LIP+WK      +  A++ L+  Q   S      KD  Q+     A +  S
Sbjct: 464 ELIDVLLVNLIPNWK------TDVAVDHLIHPQQNQS-----SKDNHQNGASSQAGESIS 523

Query: 482 SIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQ 541
             +    CP S   AN                    T + +    E E  G         
Sbjct: 524 HSLSSDYCPRSDDEAN-------------------PTVAATTEDQEAEKPG--------- 552

Query: 542 RNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAI 601
                                                     SL +++ D  LK EL  I
Sbjct: 584 ------------------------------------------SLEEEEEDERLKEELEKI 552

BLAST of Sgr025435 vs. ExPASy Swiss-Prot
Match: Q8S8Y8 (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN=WNK6 PE=2 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 6.6e-124
Identity = 242/436 (55.50%), Postives = 286/436 (65.60%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRID  LQSP  LE+LYSEV LLKSLKH NII+FYNSW+DDK KT
Sbjct: 44  KAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKT 103

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VN+ITELFTSGSLR YRKKH+ V+MKA+KNWARQ+L GL YLH   PPIIHRDLK DNIF
Sbjct: 104 VNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIF 163

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEMV
Sbjct: 164 INGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMV 223

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TF+YPY ECKN AQIYKKV+S                              GIKPASL++
Sbjct: 224 TFDYPYCECKNSAQIYKKVSS------------------------------GIKPASLSR 283

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DP+  +FI KCL+P  ERLSAKELL D FLQ+        +PL LP+     I +PK 
Sbjct: 284 VKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNG--LTMNNPLPLPD-----IVMPKE 343

Query: 302 GPI-----------------SMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEF 361
           G                   ++ ID+D   +        N GS     +E +   + N F
Sbjct: 344 GAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGS---RCIEVRRAKRGNFF 403

Query: 362 RLRGNKNDDNSVALTLRIADSSGKL---------------NMAAEMAEQLELVDHDVDFI 406
            L+G +ND+ SV+L LRI D +G++                +++EM EQLEL D +V FI
Sbjct: 404 VLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFI 439

BLAST of Sgr025435 vs. ExPASy Swiss-Prot
Match: Q944Q0 (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=1 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 4.7e-122
Identity = 274/617 (44.41%), Postives = 351/617 (56.89%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWN V I+  +Q P  LE+LYSEVHLLK+LKHENIIK + SWVD+K KT
Sbjct: 45  KAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKT 104

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           +NMITELFTSGSLR YRKKH+ VD KAIKNWARQ+L+GL YLHS NPP+IHRDLK DNIF
Sbjct: 105 INMITELFTSGSLRVYRKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIF 164

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           +NGN GEVKIGDLGLA V+QQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEMV
Sbjct: 165 VNGNTGEVKIGDLGLATVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMV 224

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           T EYPY+EC+N AQIYKKVTS                               IKP SL K
Sbjct: 225 TCEYPYNECRNQAQIYKKVTS------------------------------NIKPQSLGK 284

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DPQ  +FI KCL+P   R +A EL +D FL  +  K++    L   +   + +  P+ 
Sbjct: 285 VDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSAL--LASSSTSSKYVRPPQL 344

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
             + MD+D     H+ +    SN   P    +E Q + +N EFRLRG ++DD + ++ LR
Sbjct: 345 EHLPMDVD-----HNENKSVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVTASMVLR 404

Query: 362 IADSSGKLN---------------MAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD SGK                 +A EM E+L L   +V  IA+ ID  I +L      
Sbjct: 405 IADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQL------ 464

Query: 422 LSDYSSS--GAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPSSIIFDTGCPSSPSL 481
           LSD +SS     +  L  +D  +   + Q+     +E      + S I  D   P S   
Sbjct: 465 LSDRTSSHHNQNSPRLTHEDHEA---ANQQTVNSKDEEAAGQSMKSDISADYYFPYS--- 524

Query: 482 ANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQRNASDVGIFTPMDY 541
           AN  +   +A   +E +                    SSY+D                  
Sbjct: 525 ANDGNAAMEAGRDAESM--------------------SSYLDS----------------- 557

Query: 542 FVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRM 601
                              S+ S+  +LS++D D   +LK ELN IE+ + Q F++L ++
Sbjct: 585 ------------------CSMMSTIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKL 557

BLAST of Sgr025435 vs. ExPASy Swiss-Prot
Match: Q8RXE5 (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 GN=WNK10 PE=2 SV=1)

HSP 1 Score: 402.1 bits (1032), Expect = 1.1e-110
Identity = 258/614 (42.02%), Postives = 330/614 (53.75%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEV+GIEVAWN + I+  LQ P  L++LYSEVHLL SLKH+NIIK + SWVDD  K+
Sbjct: 32  KAFDEVEGIEVAWNLMSIEDVLQMPGQLDRLYSEVHLLNSLKHDNIIKLFYSWVDDHNKS 91

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           +NMITELFTSGSL  YRKKH+ VD KAI NWARQ+L+GL YLHS  PP+IHRDLK DNIF
Sbjct: 92  INMITELFTSGSLTLYRKKHRKVDPKAIMNWARQILKGLHYLHSQTPPVIHRDLKCDNIF 151

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           +NGN G+VKIGDLGLA VMQQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEMV
Sbjct: 152 VNGNTGKVKIGDLGLAAVMQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMV 211

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           T EYPY EC+N AQIYKKVTS                              GIKP SL+K
Sbjct: 212 TCEYPYRECRNQAQIYKKVTS------------------------------GIKPQSLSK 271

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DPQ  +FI KCL+P   R +A ELL+D  L V+  K++T          P      + 
Sbjct: 272 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASSNTTFKPAMPPQCEY 331

Query: 302 GPISMDIDIDHRQH-SLSTC--AGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVAL 361
            P    +D++++++ S+S C  A S+        +E Q + ++ EF+L G + DD + ++
Sbjct: 332 RP----MDVEYKKNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASM 391

Query: 362 TLRIADSSGKLN---------------MAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPD 421
            LRIA SSG+                 +  EM E+L+L  H+V  IAE ID LI KL   
Sbjct: 392 ALRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKLKA- 451

Query: 422 WKPLSDYSSSGAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPSSIIFDTGCPSSPS 481
                                                              +   P++ S
Sbjct: 452 ---------------------------------------------------NRSLPNANS 511

Query: 482 LANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQRNASDVGIFTPMD 541
           +   +D  +  S  SEI                          D   R +S+ G      
Sbjct: 512 VYQSKDEEAGESMKSEISA------------------------DYYHRVSSNEG------ 521

Query: 542 YFVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSR 598
                S +     +  S + S   SCS +S    +   +LK ELN IE+ Y Q  + L R
Sbjct: 572 -----SRLGCCCEAVESLLSSFLDSCSMVSNKQSE---DLKTELNVIESQYNQSCQRLLR 521

BLAST of Sgr025435 vs. ExPASy TrEMBL
Match: A0A0A0L3S7 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119370 PE=4 SV=1)

HSP 1 Score: 940.6 bits (2430), Expect = 3.1e-270
Identity = 503/662 (75.98%), Postives = 537/662 (81.12%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT
Sbjct: 103 KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 162

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 163 VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 222

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 223 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 282

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 283 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 342

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELL+DSFLQVENPKE+ R+PLQL N V +SINLPKS
Sbjct: 343 VSDPRTMEFINKCLVPVHERLSAKELLKDSFLQVENPKESARNPLQLSNQVSKSINLPKS 402

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDID + HSLST A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 403 GPISMDIDIDQKIHSLSTYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 462

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IADS+G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP+WKP
Sbjct: 463 IADSNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPEWKP 522

Query: 422 LSDYSSSGAINGL---------------------------LVEQDTSSGL-VSTQKDCLQ 481
           LS YSS+G ++                             LV QD SSGL   TQKDCLQ
Sbjct: 523 LSVYSSNGELSLFSAPPFLKSAKSSIGSAWGSILTGSHDGLVAQDISSGLGCGTQKDCLQ 582

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SEE GW  DI +  IFDT CPSSPSLANFEDL+S ASFA E+LV+D STKS         
Sbjct: 583 SEEDGWTTDISAGHIFDT-CPSSPSLANFEDLNSHASFALELLVDDCSTKSAKVFDCSNI 642

Query: 542 -------SWSIAELELHGSSY-VDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNV 601
                  SWSIAELE HGSSY V+DK QRN  DVGIFTPMDYF KNSV+S+PAPSE SNV
Sbjct: 643 DGSSKGSSWSIAELEHHGSSYVVEDKFQRNVGDVGIFTPMDYFAKNSVVSMPAPSEASNV 702

Query: 602 MSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKL 605
           MSLTSSCSSLSL DKDLDAELK+E++AIETHY+QLF+ELSRMREEALEATR+RWIAKKKL
Sbjct: 703 MSLTSSCSSLSLTDKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKL 733

BLAST of Sgr025435 vs. ExPASy TrEMBL
Match: A0A5A7U0E1 (Putative serine/threonine-protein kinase WNK7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00850 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.7e-268
Identity = 500/661 (75.64%), Postives = 532/661 (80.48%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 
Sbjct: 99  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKN 158

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 159 VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 218

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 219 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 278

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 279 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 338

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELLQDSFLQVENPKE+ R+PLQL N VPRSINLPKS
Sbjct: 339 VSDPRTMEFINKCLVPVHERLSAKELLQDSFLQVENPKESARNPLQLSNQVPRSINLPKS 398

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDIDH+ HSLS  A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 399 GPISMDIDIDHKLHSLSNYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 458

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD +G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP WKP
Sbjct: 459 IADPNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPGWKP 518

Query: 422 LSDYSSSG---------------------------AINGLLVEQDTSSGLV-STQKDCLQ 481
           LS YSS+G                             +  LV QDTSSG+V  TQ D LQ
Sbjct: 519 LSVYSSNGESSLCSAPPVLKSAKSSMRSPWGSMLTGSHDGLVAQDTSSGMVCGTQTDSLQ 578

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SE  GW DDI    IFDT CPSSPSLANFED +S ASFA E+LV+D STKS         
Sbjct: 579 SERNGWTDDISGGHIFDT-CPSSPSLANFEDSNSHASFALELLVDDCSTKSAKVFDCSNI 638

Query: 542 -------SWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVM 601
                  SWS+AELE  GSSYV+DKLQRN S VGIFTP+DYF KNSV+S+PAPSE SNVM
Sbjct: 639 DGSSKGSSWSVAELEHQGSSYVEDKLQRNVSGVGIFTPLDYFGKNSVVSMPAPSEASNVM 698

Query: 602 SLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLI 605
           SLTSSCSSLSLADKDLDAELK+E++AI THY+QLF+ELSRMREEALEATR+RWIAKKKLI
Sbjct: 699 SLTSSCSSLSLADKDLDAELKMEIDAIGTHYRQLFDELSRMREEALEATRRRWIAKKKLI 728

BLAST of Sgr025435 vs. ExPASy TrEMBL
Match: A0A5D3DB51 (Putative serine/threonine-protein kinase WNK7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00840 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.7e-268
Identity = 501/661 (75.79%), Postives = 532/661 (80.48%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 
Sbjct: 99  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKN 158

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 159 VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 218

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 219 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 278

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 279 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 338

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELLQDSFLQVENPKE+ R+PLQL N V RSINLPKS
Sbjct: 339 VSDPRTMEFINKCLVPVHERLSAKELLQDSFLQVENPKESARNPLQLSNQVSRSINLPKS 398

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDIDH+ HSLS  A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 399 GPISMDIDIDHKLHSLSNYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 458

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD +G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP WKP
Sbjct: 459 IADPNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPGWKP 518

Query: 422 LSDYSSSG---------------------------AINGLLVEQDTSSGLV-STQKDCLQ 481
           LS YSS+G                             +  LV QDTSSG+V  TQ D LQ
Sbjct: 519 LSVYSSNGESSLCSAPPVLKSAKSSVRSPWGSMLTGSHDGLVAQDTSSGMVCGTQTDSLQ 578

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SE  GW  DI    IFDT CPSSPSLANFED +S ASFA E+LV+D STKS         
Sbjct: 579 SERNGWTGDISGGHIFDT-CPSSPSLANFEDSNSHASFALELLVDDCSTKSAKVFDCSNI 638

Query: 542 -------SWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVM 601
                  SWSIAELE  GSSYV+DKLQRN S VGIFTP+DYFVKNSV+S+PAPSE SNVM
Sbjct: 639 DGSSKGSSWSIAELEHQGSSYVEDKLQRNVSGVGIFTPLDYFVKNSVVSMPAPSEASNVM 698

Query: 602 SLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLI 605
           SLTSSCSSLSLADKDLDAELK+E++AIETHY+QLF+ELSRMREEALEATR+RWIAKKKLI
Sbjct: 699 SLTSSCSSLSLADKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLI 728

BLAST of Sgr025435 vs. ExPASy TrEMBL
Match: A0A1S3AVK7 (probable serine/threonine-protein kinase WNK7 OS=Cucumis melo OX=3656 GN=LOC103483165 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.7e-268
Identity = 501/661 (75.79%), Postives = 532/661 (80.48%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKK 
Sbjct: 33  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKN 92

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQ+LRGLVYLHSH+PPIIHRDLKGDNIF
Sbjct: 93  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQILRGLVYLHSHDPPIIHRDLKGDNIF 152

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 153 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 212

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPYSECKNPAQI++KVTS                              GIKPASLAK
Sbjct: 213 TFEYPYSECKNPAQIFRKVTS------------------------------GIKPASLAK 272

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           VSDP+TMEFINKCLVPVHERLSAKELLQDSFLQVENPKE+ R+PLQL N V RSINLPKS
Sbjct: 273 VSDPRTMEFINKCLVPVHERLSAKELLQDSFLQVENPKESARNPLQLSNQVSRSINLPKS 332

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
           GPISMDIDIDH+ HSLS  A SN GSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR
Sbjct: 333 GPISMDIDIDHKLHSLSNYAESNSGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 392

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD +G+               L++AAEMAEQLEL++HDVDFIAEFIDFLI KLIP WKP
Sbjct: 393 IADPNGRVRNIHFTFYLDSDTALSVAAEMAEQLELINHDVDFIAEFIDFLITKLIPGWKP 452

Query: 422 LSDYSSSG---------------------------AINGLLVEQDTSSGLV-STQKDCLQ 481
           LS YSS+G                             +  LV QDTSSG+V  TQ D LQ
Sbjct: 453 LSVYSSNGESSLCSAPPVLKSAKSSVRSPWGSMLTGSHDGLVAQDTSSGMVCGTQTDSLQ 512

Query: 482 SEEVGWADDIPSSIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSV-------- 541
           SE  GW  DI    IFDT CPSSPSLANFED +S ASFA E+LV+D STKS         
Sbjct: 513 SERNGWTGDISGGHIFDT-CPSSPSLANFEDSNSHASFALELLVDDCSTKSAKVFDCSNI 572

Query: 542 -------SWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVM 601
                  SWSIAELE  GSSYV+DKLQRN S VGIFTP+DYFVKNSV+S+PAPSE SNVM
Sbjct: 573 DGSSKGSSWSIAELEHQGSSYVEDKLQRNVSGVGIFTPLDYFVKNSVVSMPAPSEASNVM 632

Query: 602 SLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKLI 605
           SLTSSCSSLSLADKDLDAELK+E++AIETHY+QLF+ELSRMREEALEATR+RWIAKKKLI
Sbjct: 633 SLTSSCSSLSLADKDLDAELKMEIDAIETHYRQLFDELSRMREEALEATRRRWIAKKKLI 662

BLAST of Sgr025435 vs. ExPASy TrEMBL
Match: A0A6J1D2B8 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111016354 PE=4 SV=1)

HSP 1 Score: 934.1 bits (2413), Expect = 2.9e-268
Identity = 507/662 (76.59%), Postives = 535/662 (80.82%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDK KT
Sbjct: 33  KAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKNKT 92

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSH+PPI+HRDLKGDNIF
Sbjct: 93  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHDPPIVHRDLKGDNIF 152

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV
Sbjct: 153 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 212

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
            FEYPYSECKNPAQIYKKVTS                              GIKP+SLAK
Sbjct: 213 AFEYPYSECKNPAQIYKKVTS------------------------------GIKPSSLAK 272

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V+DPQTMEFINKCLVP  ERLSA ELL+D FL+VENPKE+TRDPLQLPN +PRSINL KS
Sbjct: 273 VTDPQTMEFINKCLVPATERLSATELLKDPFLRVENPKESTRDPLQLPNLIPRSINLMKS 332

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
            PISMDIDIDHRQ+SLSTCAGS+G SPR PV+EFQT NKN+EFRLRGNKNDDNSVALTLR
Sbjct: 333 EPISMDIDIDHRQNSLSTCAGSSGESPRLPVMEFQTKNKNHEFRLRGNKNDDNSVALTLR 392

Query: 362 IADSSGK---------------LNMAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IADS+G+               L++AAEMAEQLELV HDVDFIAEFIDF+I KLIP WKP
Sbjct: 393 IADSNGRVKNIHFTFYLDSDTALSVAAEMAEQLELVTHDVDFIAEFIDFVITKLIPGWKP 452

Query: 422 LSDYSSSGAI------------------------NGL---LVEQDTSSGLVSTQKDCLQS 481
           LSDYSSS AI                        +G+   LVEQD SSGLVSTQKDCLQS
Sbjct: 453 LSDYSSSEAISLCGGPYILTNTKSSVPSTCNSVLSGVLDGLVEQDVSSGLVSTQKDCLQS 512

Query: 482 EEVGWADDIPSSIIFDTGCPSSPSLANFEDL-HSQASFASEILVEDSST----------- 541
           EE GW D  P S IF+TG   SPSL NFED+  SQASFASE LVEDSST           
Sbjct: 513 EEEGWTDGFPDSHIFNTGGCPSPSLGNFEDIPDSQASFASETLVEDSSTTIGKVIDCSNI 572

Query: 542 ----KSVSWSIAELELHGSSYVDDKLQRNASDVGIFTPMDYFVKNSVMSLPAPSEVS-NV 601
               K  SWSIA+LELHGSSYVDDKLQ N SDVGIFTPMDYFVKNSVMSLP PS VS NV
Sbjct: 573 DGSSKGSSWSIADLELHGSSYVDDKLQINGSDVGIFTPMDYFVKNSVMSLPLPSGVSTNV 632

Query: 602 MSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRMREEALEATRKRWIAKKKL 605
           MSLTSSCSSLSLADKDL+AE KLELNA+E +YQQLFEE+SRMREEALEATRKRWI +KKL
Sbjct: 633 MSLTSSCSSLSLADKDLEAEFKLELNAVEMYYQQLFEEVSRMREEALEATRKRWI-EKKL 663

BLAST of Sgr025435 vs. TAIR 10
Match: AT1G49160.1 (Protein kinase superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 5.0e-127
Identity = 281/630 (44.60%), Postives = 352/630 (55.87%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           + FDEVDGIEVAWNQVRID  LQSP+ LE+LYSEV LLKSLKH+NII+FYNSW+DDK KT
Sbjct: 26  KGFDEVDGIEVAWNQVRIDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKT 85

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VN+ITELFTSGSLRQYRKKH+ V+MKA+K WARQ+L GL YLHS +PPIIHRD+K DNIF
Sbjct: 86  VNIITELFTSGSLRQYRKKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIF 145

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEMV
Sbjct: 146 INGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMV 205

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPY EC+N AQIYKKV+S                              GIKPASL+K
Sbjct: 206 TFEYPYCECRNSAQIYKKVSS------------------------------GIKPASLSK 265

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DP+ M+FI KCL+P  ERLSA+ELL DSFL V        +PL LP+     I +PK 
Sbjct: 266 VKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNG--LVMNNPLPLPD-----IVMPKE 325

Query: 302 GPI----------------SMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFR 361
           G                  +M +++D   +     + +N G+     +E +   + N F 
Sbjct: 326 GSFGERCLMSEGPPNARNRTMSMNLDEDNNLPIVISSNNSGT---NCIEVRRAKRGNFFV 385

Query: 362 LRGNKNDDNSVALTLRIADSSGKL---------------NMAAEMAEQLELVDHDVDFIA 421
           L+G +ND+NSV+L LRI D +G++               N+++EM EQLEL D +V FIA
Sbjct: 386 LKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVKFIA 445

Query: 422 EFIDFLIAKLIPDWKPLSDYSSSGAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPS 481
           E ID L+  LIP+WK      +  A++ L+  Q   S      KD  Q+     A +  S
Sbjct: 446 ELIDVLLVNLIPNWK------TDVAVDHLIHPQQNQS-----SKDNHQNGASSQAGESIS 505

Query: 482 SIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQ 541
             +    CP S   AN                    T + +    E E  G         
Sbjct: 506 HSLSSDYCPRSDDEAN-------------------PTVAATTEDQEAEKPG--------- 534

Query: 542 RNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAI 601
                                                     SL +++ D  LK EL  I
Sbjct: 566 ------------------------------------------SLEEEEEDERLKEELEKI 534

BLAST of Sgr025435 vs. TAIR 10
Match: AT1G49160.2 (Protein kinase superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 5.0e-127
Identity = 281/630 (44.60%), Postives = 352/630 (55.87%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           + FDEVDGIEVAWNQVRID  LQSP+ LE+LYSEV LLKSLKH+NII+FYNSW+DDK KT
Sbjct: 44  KGFDEVDGIEVAWNQVRIDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKT 103

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VN+ITELFTSGSLRQYRKKH+ V+MKA+K WARQ+L GL YLHS +PPIIHRD+K DNIF
Sbjct: 104 VNIITELFTSGSLRQYRKKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIF 163

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEMV
Sbjct: 164 INGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMV 223

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TFEYPY EC+N AQIYKKV+S                              GIKPASL+K
Sbjct: 224 TFEYPYCECRNSAQIYKKVSS------------------------------GIKPASLSK 283

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DP+ M+FI KCL+P  ERLSA+ELL DSFL V        +PL LP+     I +PK 
Sbjct: 284 VKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNG--LVMNNPLPLPD-----IVMPKE 343

Query: 302 GPI----------------SMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFR 361
           G                  +M +++D   +     + +N G+     +E +   + N F 
Sbjct: 344 GSFGERCLMSEGPPNARNRTMSMNLDEDNNLPIVISSNNSGT---NCIEVRRAKRGNFFV 403

Query: 362 LRGNKNDDNSVALTLRIADSSGKL---------------NMAAEMAEQLELVDHDVDFIA 421
           L+G +ND+NSV+L LRI D +G++               N+++EM EQLEL D +V FIA
Sbjct: 404 LKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELTDKNVKFIA 463

Query: 422 EFIDFLIAKLIPDWKPLSDYSSSGAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPS 481
           E ID L+  LIP+WK      +  A++ L+  Q   S      KD  Q+     A +  S
Sbjct: 464 ELIDVLLVNLIPNWK------TDVAVDHLIHPQQNQS-----SKDNHQNGASSQAGESIS 523

Query: 482 SIIFDTGCPSSPSLANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQ 541
             +    CP S   AN                    T + +    E E  G         
Sbjct: 524 HSLSSDYCPRSDDEAN-------------------PTVAATTEDQEAEKPG--------- 552

Query: 542 RNASDVGIFTPMDYFVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAI 601
                                                     SL +++ D  LK EL  I
Sbjct: 584 ------------------------------------------SLEEEEEDERLKEELEKI 552

BLAST of Sgr025435 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 446.0 bits (1146), Expect = 4.7e-125
Identity = 242/436 (55.50%), Postives = 286/436 (65.60%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRID  LQSP  LE+LYSEV LLKSLKH NII+FYNSW+DDK KT
Sbjct: 44  KAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKT 103

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VN+ITELFTSGSLR YRKKH+ V+MKA+KNWARQ+L GL YLH   PPIIHRDLK DNIF
Sbjct: 104 VNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIF 163

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEMV
Sbjct: 164 INGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMV 223

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TF+YPY ECKN AQIYKKV+S                              GIKPASL++
Sbjct: 224 TFDYPYCECKNSAQIYKKVSS------------------------------GIKPASLSR 283

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DP+  +FI KCL+P  ERLSAKELL D FLQ+        +PL LP+     I +PK 
Sbjct: 284 VKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNG--LTMNNPLPLPD-----IVMPKE 343

Query: 302 GPI-----------------SMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEF 361
           G                   ++ ID+D   +        N GS     +E +   + N F
Sbjct: 344 GAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGS---RCIEVRRAKRGNFF 403

Query: 362 RLRGNKNDDNSVALTLRIADSSGKL---------------NMAAEMAEQLELVDHDVDFI 406
            L+G +ND+ SV+L LRI D +G++                +++EM EQLEL D +V FI
Sbjct: 404 VLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFI 439

BLAST of Sgr025435 vs. TAIR 10
Match: AT3G18750.3 (with no lysine (K) kinase 6 )

HSP 1 Score: 446.0 bits (1146), Expect = 4.7e-125
Identity = 242/436 (55.50%), Postives = 286/436 (65.60%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWNQVRID  LQSP  LE+LYSEV LLKSLKH NII+FYNSW+DDK KT
Sbjct: 44  KAFDEVDGIEVAWNQVRIDDVLQSPNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKT 103

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           VN+ITELFTSGSLR YRKKH+ V+MKA+KNWARQ+L GL YLH   PPIIHRDLK DNIF
Sbjct: 104 VNIITELFTSGSLRHYRKKHRKVNMKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIF 163

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           INGNHGEVKIGDLGLA VM+Q  A+SVIGTPEFMAPELY+E YNEL D+YSFGMCMLEMV
Sbjct: 164 INGNHGEVKIGDLGLATVMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMV 223

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           TF+YPY ECKN AQIYKKV+S                              GIKPASL++
Sbjct: 224 TFDYPYCECKNSAQIYKKVSS------------------------------GIKPASLSR 283

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DP+  +FI KCL+P  ERLSAKELL D FLQ+        +PL LP+     I +PK 
Sbjct: 284 VKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNG--LTMNNPLPLPD-----IVMPKE 343

Query: 302 GPI-----------------SMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEF 361
           G                   ++ ID+D   +        N GS     +E +   + N F
Sbjct: 344 GAFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGS---RCIEVRRAKRGNFF 403

Query: 362 RLRGNKNDDNSVALTLRIADSSGKL---------------NMAAEMAEQLELVDHDVDFI 406
            L+G +ND+ SV+L LRI D +G++                +++EM EQLEL D +V FI
Sbjct: 404 VLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQLELTDQNVTFI 439

BLAST of Sgr025435 vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 439.9 bits (1130), Expect = 3.3e-123
Identity = 274/617 (44.41%), Postives = 351/617 (56.89%), Query Frame = 0

Query: 2   QAFDEVDGIEVAWNQVRIDGFLQSPEDLEKLYSEVHLLKSLKHENIIKFYNSWVDDKKKT 61
           +AFDEVDGIEVAWN V I+  +Q P  LE+LYSEVHLLK+LKHENIIK + SWVD+K KT
Sbjct: 45  KAFDEVDGIEVAWNLVSIEDVMQMPGQLERLYSEVHLLKALKHENIIKLFYSWVDEKNKT 104

Query: 62  VNMITELFTSGSLRQYRKKHKHVDMKAIKNWARQVLRGLVYLHSHNPPIIHRDLKGDNIF 121
           +NMITELFTSGSLR YRKKH+ VD KAIKNWARQ+L+GL YLHS NPP+IHRDLK DNIF
Sbjct: 105 INMITELFTSGSLRVYRKKHRKVDPKAIKNWARQILKGLNYLHSQNPPVIHRDLKCDNIF 164

Query: 122 INGNHGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYEEEYNELVDVYSFGMCMLEMV 181
           +NGN GEVKIGDLGLA V+QQPTARSVIGTPEFMAPELYEEEYNELVD+YSFGMCMLEMV
Sbjct: 165 VNGNTGEVKIGDLGLATVLQQPTARSVIGTPEFMAPELYEEEYNELVDIYSFGMCMLEMV 224

Query: 182 TFEYPYSECKNPAQIYKKVTSVSETIVTSSSCNMLTVLADHYSNTDLLLNQGIKPASLAK 241
           T EYPY+EC+N AQIYKKVTS                               IKP SL K
Sbjct: 225 TCEYPYNECRNQAQIYKKVTS------------------------------NIKPQSLGK 284

Query: 242 VSDPQTMEFINKCLVPVHERLSAKELLQDSFLQVENPKEATRDPLQLPNHVPRSINLPKS 301
           V DPQ  +FI KCL+P   R +A EL +D FL  +  K++    L   +   + +  P+ 
Sbjct: 285 VDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSAL--LASSSTSSKYVRPPQL 344

Query: 302 GPISMDIDIDHRQHSLSTCAGSNGGSPRFPVVEFQTMNKNNEFRLRGNKNDDNSVALTLR 361
             + MD+D     H+ +    SN   P    +E Q + +N EFRLRG ++DD + ++ LR
Sbjct: 345 EHLPMDVD-----HNENKSVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVTASMVLR 404

Query: 362 IADSSGKLN---------------MAAEMAEQLELVDHDVDFIAEFIDFLIAKLIPDWKP 421
           IAD SGK                 +A EM E+L L   +V  IA+ ID  I +L      
Sbjct: 405 IADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQL------ 464

Query: 422 LSDYSSS--GAINGLLVEQDTSSGLVSTQKDCLQSEEVGWADDIPSSIIFDTGCPSSPSL 481
           LSD +SS     +  L  +D  +   + Q+     +E      + S I  D   P S   
Sbjct: 465 LSDRTSSHHNQNSPRLTHEDHEA---ANQQTVNSKDEEAAGQSMKSDISADYYFPYS--- 524

Query: 482 ANFEDLHSQASFASEILVEDSSTKSVSWSIAELELHGSSYVDDKLQRNASDVGIFTPMDY 541
           AN  +   +A   +E +                    SSY+D                  
Sbjct: 525 ANDGNAAMEAGRDAESM--------------------SSYLDS----------------- 557

Query: 542 FVKNSVMSLPAPSEVSNVMSLTSSCSSLSLADKDLDAELKLELNAIETHYQQLFEELSRM 601
                              S+ S+  +LS++D D   +LK ELN IE+ + Q F++L ++
Sbjct: 585 ------------------CSMMSTIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKL 557

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893325.15.7e-27477.00probable serine/threonine-protein kinase WNK7 isoform X1 [Benincasa hispida][more]
XP_004134295.16.5e-27075.98probable serine/threonine-protein kinase WNK7 isoform X1 [Cucumis sativus] >KAE8... [more]
XP_011650666.16.5e-27075.98probable serine/threonine-protein kinase WNK7 isoform X2 [Cucumis sativus][more]
XP_008437860.13.5e-26875.79PREDICTED: probable serine/threonine-protein kinase WNK7 [Cucumis melo][more]
KAA0048870.13.5e-26875.64putative serine/threonine-protein kinase WNK7 [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q6EU497.5e-13648.09Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX... [more]
Q8LST27.0e-12644.60Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8S8Y86.6e-12455.50Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q944Q04.7e-12244.41Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=... [more]
Q8RXE51.1e-11042.02Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A0A0L3S73.1e-27075.98Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119... [more]
A0A5A7U0E11.7e-26875.64Putative serine/threonine-protein kinase WNK7 OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3DB511.7e-26875.79Putative serine/threonine-protein kinase WNK7 OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3AVK71.7e-26875.79probable serine/threonine-protein kinase WNK7 OS=Cucumis melo OX=3656 GN=LOC1034... [more]
A0A6J1D2B82.9e-26876.59Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
Match NameE-valueIdentityDescription
AT1G49160.15.0e-12744.60Protein kinase superfamily protein [more]
AT1G49160.25.0e-12744.60Protein kinase superfamily protein [more]
AT3G18750.14.7e-12555.50with no lysine (K) kinase 6 [more]
AT3G18750.34.7e-12555.50with no lysine (K) kinase 6 [more]
AT5G41990.13.3e-12344.41with no lysine (K) kinase 8 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 567..594
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 69..279
e-value: 7.2E-49
score: 167.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 1..67
e-value: 1.6E-19
score: 71.7
NoneNo IPR availablePIRSRPIRSR000620-1PIRSR000620-1coord: 31..200
e-value: 2.3E-7
score: 28.0
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 232..412
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 2..202
coord: 418..602
NoneNo IPR availablePANTHERPTHR13902:SF134KINASE DOMAIN PROTEINcoord: 232..412
coord: 2..202
coord: 418..602
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 1..273
e-value: 2.6E-47
score: 173.2
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 27..273
e-value: 7.1E-49
score: 166.5
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 1..273
score: 37.558998
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 110..122
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..277

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025435.1Sgr025435.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity