Sgr025418 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025418 vs. NCBI nr
Match: XP_022147438.1 (cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 605/720 (84.03%), Postives = 648/720 (90.00%), Query Frame = 0

Query: 263 MSKFMSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEG 322
           MSKFM S KPKS RF +P      TSD HV+YG YQNRSRLSP S+LESIQR++SSA EG
Sbjct: 1   MSKFMGSLKPKSLRFGYP------TSDIHVRYGGYQNRSRLSPASILESIQRKISSASEG 60

Query: 323 FRSMRAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIP 382
           FR M A L  KH  DQS KKP FWKK LDPQ+PFLQQWNKIFVLSCV+AVAVDPLFFYIP
Sbjct: 61  FRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIP 120

Query: 383 VIDGQHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPA 442
           V+D QH+CLTMDR LMIIACVLRSFIDIFYLLH+IFEFRTGF+ PSLPVFGSG+LVEDPA
Sbjct: 121 VMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPA 180

Query: 443 AIAKRYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRI 502
           AIAKRYLSSNFIIDILSI+PLPQLL LVI+PAAKGPIPLKTK+F+K+AVLLQY+PRL RI
Sbjct: 181 AIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRI 240

Query: 503 YPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNN 562
           YPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFS+EQ++RCW EAC++NN
Sbjct: 241 YPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKNN 300

Query: 563 CASNYLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLT-ETTNFRRKFFY 622
           C S YLYCGDH RPAY FI  YCPHKE EDD +FNFG+Y+EALKF LT +TTNF  KFFY
Sbjct: 301 CNSKYLYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFY 360

Query: 623 CFWWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQM 682
           CFWWALRNVGSSGQNLQISNYLGEL FAVFIAILGLVLFALLI+NIQKYLQSTTVK+EQM
Sbjct: 361 CFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQM 420

Query: 683 RINRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLC 742
           RINRRDAEHWM HRMLPED+RQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDIKRHLC
Sbjct: 421 RINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLC 480

Query: 743 LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTY 802
           LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKS ILQEGDP+D MLFIMKG LATI TY
Sbjct: 481 LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITY 540

Query: 803 GGKKDSYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVA 862
           G K DS+SDVLKAGDFCGEE+VPWAMDP STSLPISTRTVK+LTEVEAFALKA ELQSV 
Sbjct: 541 GWKNDSHSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVV 600

Query: 863 SQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDAL 922
           S F F R +SQQFQLSVRF+SHQWKVWAAYKIQEAWHDYCERK++      GD RF+ AL
Sbjct: 601 SLFRFQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRR----GGGDSRFQGAL 660

Query: 923 AKTVGASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDEPDFTILDL 981
           AKTVGASASFGA LYASIFISHLLQ VQ+ +HQQT+Q +RVMTLPPPPKP E DFTILDL
Sbjct: 661 AKTVGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL 710

BLAST of Sgr025418 vs. NCBI nr
Match: XP_022147437.1 (LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like [Momordica charantia])

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 598/725 (82.48%), Postives = 641/725 (88.41%), Query Frame = 0

Query: 261 ILMSKFMSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAY 320
           +LMSKFM S KPK  RF +P      TSD HVKYG YQNRS LSP S+LESIQRR SSA 
Sbjct: 1   MLMSKFMGSLKPKFFRFGYP------TSDIHVKYGGYQNRSTLSPASILESIQRRFSSAS 60

Query: 321 EGFRSMRAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFY 380
           EGFR M AVL  KH  DQ  KK    KK LDPQ+PFLQQWNKIFVLSCV+AVAVDPLFFY
Sbjct: 61  EGFRRMEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFY 120

Query: 381 IPVIDGQHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVED 440
           IPVID QH+CLTMDR LMIIAC+LRSFIDIFYLLH+IFEFRTGF+ PSLPVFGSG+LVED
Sbjct: 121 IPVIDRQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVED 180

Query: 441 PAAIAKRYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLL 500
           PAAIAKRYLSSNFIIDILSI+PLPQLLVLVI+PAAKGPI L+TKDF+K+AVLLQYIPRLL
Sbjct: 181 PAAIAKRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLL 240

Query: 501 RIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKR 560
           RIY LYREVTRTSGILTE AWSGAAFNLLIYMLASHVVGAIWYLFS+EQ+ RCWLEAC+ 
Sbjct: 241 RIYLLYREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRT 300

Query: 561 NNCASNYLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLT-ETTNFRRKF 620
            NC S  L CGD  R AY  I +YC HK+ EDD +FNFGMY+EALKFHLT +TTNFR KF
Sbjct: 301 RNCNSKDLCCGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKF 360

Query: 621 FYCFWWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIE 680
           FYCFWWALRNVGS GQNLQISNYLGE+LFAVFIAILGL+LFALLI+NIQKYLQSTTVK+E
Sbjct: 361 FYCFWWALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVE 420

Query: 681 QMRINRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRH 740
           QMRI+RRDAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGV+EEELISNLPKDLRRDI RH
Sbjct: 421 QMRISRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRH 480

Query: 741 LCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIF 800
           LCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTE S ILQEGDPID MLFIMKG LATI 
Sbjct: 481 LCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATII 540

Query: 801 TYGGKKDSYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQS 860
           TYG K DS+SDVLKAGDFCGEE+VPWAMDP STSLPI TRTVK+LTEVEAFALKA ELQS
Sbjct: 541 TYGWKNDSHSDVLKAGDFCGEELVPWAMDPQSTSLPIXTRTVKTLTEVEAFALKAKELQS 600

Query: 861 VASQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRD 920
           V SQFHF R +SQQFQLSVRF+SHQWKVWAAYKIQEAWHDYCERK++      GDGRF+D
Sbjct: 601 VVSQFHFQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRR----GGGDGRFQD 660

Query: 921 ALAKTVGASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKP---DEPDF 980
           ALAKT GASASFGA LYASIFISHLLQ VQ+ +HQQT+Q +RVMTLPPPPKP   D+ DF
Sbjct: 661 ALAKTAGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDF 714

BLAST of Sgr025418 vs. NCBI nr
Match: XP_022980030.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980031.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980033.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima] >XP_022980034.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 568/719 (79.00%), Postives = 629/719 (87.48%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M SQ PKSARF HP      TSDFH KYG +QNRSRLSP S+LESI+R+ SSAY+  R +
Sbjct: 1   MGSQIPKSARFGHP------TSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRI 60

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
              L  KH GDQS KKP FWKK LDPQ+PFLQQWNKIFVLS VIAVAVDPLFFYIPVIDG
Sbjct: 61  ITHLSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDG 120

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           + +CLT+DR LMIIACVLRS +D+FY+LHMIFEFRTGF+ PSLPVFGSG+L+EDPAAIAK
Sbjct: 121 ERQCLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAK 180

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL SNF+IDILS+LPLPQLLVL I+PAAKGPIPLKTKD +KIAVLLQY+PRLLRIYPLY
Sbjct: 181 RYLLSNFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLY 240

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNNCASN 566
           REVTRT GILTETAWSGAAFNLLIYM  SHVVGAIWYL S+E+QA+CW+EAC+++ C  N
Sbjct: 241 REVTRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDN 300

Query: 567 YLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWA 626
           +LYCG+ RR   P+ID+YC  KEPED  +FNFGMY+EALKF LT TT+FRRKFFY FWWA
Sbjct: 301 FLYCGNQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWA 360

Query: 627 LRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRR 686
           LRNV SSGQNLQ+SNY+GE+ FAVFIAILGLVLFALLI+NIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRR 420

Query: 687 DAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 746
           DAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLK
Sbjct: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLK 480

Query: 747 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKD 806
           KVPLFS MDKQLLDAMCEYLKPVLFTEKS+ILQEGDPID MLFIMKGKLATI TYG K D
Sbjct: 481 KVPLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKND 540

Query: 807 SYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVASQFHF 866
           ++S VL AGDFCGEE+V WA DP STSLPIS+RTVKSLTEVEAFALKANEL+SV SQFHF
Sbjct: 541 TFSAVLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHF 600

Query: 867 HRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVG 926
            RL+S+QFQLSVRF+SHQW VWAAYKIQEAW +Y ERK++  GV  GDGRF+ ALAKT G
Sbjct: 601 QRLNSKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRR--GV-RGDGRFQAALAKTAG 660

Query: 927 ASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDE----PDFTILDL 981
           AS  FGA LYASIFISHLLQ VQ+ +H++T Q  RVMTLPPPPKPD+    P+ TIL+L
Sbjct: 661 ASVGFGATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 710

BLAST of Sgr025418 vs. NCBI nr
Match: XP_022924449.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >XP_022924450.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita moschata] >KAG6582669.1 Cyclic nucleotide-gated ion channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 563/718 (78.41%), Postives = 626/718 (87.19%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M SQ PKS RF HP      TSDFH KYG YQN SRLSP S+LESI+R+  SAY+ FR +
Sbjct: 1   MGSQIPKSTRFGHP------TSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRI 60

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
              L  KH GDQS KKP FWKK LDPQ+PFLQQWNKIFVLS VIAVAVDPLFFYIPVI+G
Sbjct: 61  ITHLSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVING 120

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           + +CLT+DR LMIIACVLRS +D+FY+LHMIFEFRTGF+ PSLPVFGSG+L+EDPAAIAK
Sbjct: 121 ERQCLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAK 180

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL SNF+IDILS+LPLPQLLVL I+PAA+GP+PLKTKD +KIAVLLQY+PRLLRIYPLY
Sbjct: 181 RYLFSNFLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLY 240

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNNCASN 566
           REVTRT GILTETAWSGAAFNLLIYM  SHVVGAIWYL S+E+QA+CW+EAC+++ C  N
Sbjct: 241 REVTRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDN 300

Query: 567 YLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWA 626
           +LYCG+ RR   P+ID+YC  KE ED  +FNFGMY+EALKF LT TT+FRRKFFY FWWA
Sbjct: 301 FLYCGNQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWA 360

Query: 627 LRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRR 686
           LRNV SSGQNLQ+SNY+GE+ FAVFIAILGLVLFALLI+NIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRR 420

Query: 687 DAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 746
           DAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLK
Sbjct: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLK 480

Query: 747 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKD 806
           KVPLFS MDKQLLDAMCEYLKPVLFTEKS+ILQEGDPID +LFIMKGKLATI TYG K D
Sbjct: 481 KVPLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKND 540

Query: 807 SYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVASQFHF 866
           + S VL AGDFCGEE+V WAMDP STSLPIS+RTVKSLTEVEAFALKANEL+SV SQFHF
Sbjct: 541 TLSGVLNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHF 600

Query: 867 HRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVG 926
             L+S+QFQLSVRF+SHQW VWAAYK+QEAW DY ERK++  GV  GDGRF+DALAKT G
Sbjct: 601 QHLNSKQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRR--GV-RGDGRFQDALAKTAG 660

Query: 927 ASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDE----PDFTILD 980
           AS  FGA LYASIFISHLLQ VQ+ +HQ+T Q  +VMTLPPPPKPD+    P+FTIL+
Sbjct: 661 ASVGFGATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of Sgr025418 vs. NCBI nr
Match: XP_023526877.1 (cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526878.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023526879.1 cyclic nucleotide-gated ion channel 1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1100.1 bits (2844), Expect = 0.0e+00
Identity = 559/718 (77.86%), Postives = 623/718 (86.77%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M SQ PKS RF HP      TSDFH KYG YQN SRLSP S+LESI+R+ SSAY+  R +
Sbjct: 1   MGSQIPKSTRFGHP------TSDFHDKYGSYQNGSRLSPGSILESIERKFSSAYKSSRRI 60

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
                 KH GDQS KKP F KK LDPQ+PFLQQWNKIFVLS VIAVAVDPLFFYIPVI+G
Sbjct: 61  ITHFSFKHPGDQSVKKPKFRKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVING 120

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           + +CLT+DR LMIIACVLRS +D+FY+LHMIFEFRTGF+ PSLPVFGSG+L+EDPAAIAK
Sbjct: 121 ERQCLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAK 180

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL SNF+IDILS+LPLPQLLVL I+PAAKGPIPLKTKD +KIAVLLQY+PRLLRIYPLY
Sbjct: 181 RYLFSNFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLY 240

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNNCASN 566
           REVTRT GILTETAWSGAAFNLLIYM  SHVVGAIWYL S+E+QA+CW+EAC++  C  N
Sbjct: 241 REVTRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKGKCKDN 300

Query: 567 YLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWA 626
           +LYCG+ RR  +P+ID+YC  KE ED+ +F FGM++EALKF LT TT+FRRKFFY FWWA
Sbjct: 301 FLYCGNQRRQIHPYIDQYCSPKESEDEKTFKFGMFVEALKFQLTSTTSFRRKFFYSFWWA 360

Query: 627 LRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRR 686
           LRNV SSGQNLQ+SNY+G++ FAVFIAILGLVLFALLI+NIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVSSSGQNLQVSNYMGDVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRR 420

Query: 687 DAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 746
           DAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLK
Sbjct: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLK 480

Query: 747 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKD 806
           KVPLFS MDKQLLDAMCEYLKPVLFTEKS+ILQEGDPID MLFIMKGKLAT  TYG K +
Sbjct: 481 KVPLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATSITYGWKNN 540

Query: 807 SYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVASQFHF 866
           ++S VL AGDFCGEE+V WA DP STSLPIS+RTVKSLTEVEAFALKANEL+SV SQFHF
Sbjct: 541 TFSGVLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHF 600

Query: 867 HRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVG 926
            RL+S+QFQLSVRF+SHQW VWAAYKIQEAW +Y ERK++  GV  GDGRF+DALAK  G
Sbjct: 601 QRLNSKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRR--GV-RGDGRFQDALAKMAG 660

Query: 927 ASASFGAILYASIFISHLLQAVQQEH-QQTAQPARVMTLPPPPKPDE----PDFTILD 980
           AS  FGA LYASIFISHLLQ VQ++  Q+TAQ  RVMTLPPPPKPD+    P+FTIL+
Sbjct: 661 ASVGFGATLYASIFISHLLQVVQRDQPQETAQLTRVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of Sgr025418 vs. ExPASy Swiss-Prot
Match: O65717 (Cyclic nucleotide-gated ion channel 1 OS=Arabidopsis thaliana OX=3702 GN=CNGC1 PE=1 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 8.8e-211
Identity = 388/717 (54.11%), Postives = 519/717 (72.38%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTS-DFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRS 326
           M+ ++ K  RF    + + S+  ++  K  +     + +  S+ +   R   S+    + 
Sbjct: 1   MNFRQEKFVRFQDWKSDKTSSDVEYSGKNEIQTGIFQRTISSISDKFYRSFESSSARIKL 60

Query: 327 MRAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVID 386
            +   +S    +  +K  G   K LDPQ PFLQ+WNKIFVL+C+IAV++DPLFFY+P+ID
Sbjct: 61  FKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIID 120

Query: 387 GQHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIA 446
              KCL +D+ + I A VLRSF D+FY+LH+IF+FRTGF+ PS  VFG G LVED   IA
Sbjct: 121 DAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIA 180

Query: 447 KRYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPL 506
           KRYLSS+FIIDIL++LPLPQ+++L+I+P  +G   L TK+ +K  V  QYIPR +RIYPL
Sbjct: 181 KRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNTKNMLKFIVFFQYIPRFIRIYPL 240

Query: 507 YREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNN--C 566
           Y+EVTRTSGILTETAW+GAAFNL +YMLASHV GA WYLFSIE++  CW +AC+RNN  C
Sbjct: 241 YKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPC 300

Query: 567 ASNYLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCF 626
            S  LYC         F++  CP + P +   F+FG++++AL+  + E+ +F +KFFYCF
Sbjct: 301 ISKLLYCDPETAGGNAFLNESCPIQTP-NTTLFDFGIFLDALQSGVVESQDFPQKFFYCF 360

Query: 627 WWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRI 686
           WW L+N+ S GQNL+ S Y+ E+ FAVFI+I GLVLF+ LI N+Q YLQSTT ++E+MR+
Sbjct: 361 WWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEMRV 420

Query: 687 NRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLA 746
            RRDAE WM+HR+LPE++R+RIRRY+QYKWQ  RGV EE L+SNLPKDLRRDIKRHLCLA
Sbjct: 421 KRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLCLA 480

Query: 747 LLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGG 806
           LL +VP+F  MD+QLLDA+C+ L+PVL+TE+SYI++EGDP+D MLFIM+GKL TI T GG
Sbjct: 481 LLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTNGG 540

Query: 807 KKDSY-SDVLKAGDFCGEEVVPWAMDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVA 866
           +     S+ L AGDFCGEE++ WA+DPHS+S LPISTRTV++L EVEAFALKA++L+ VA
Sbjct: 541 RTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTRTVRALMEVEAFALKADDLKFVA 600

Query: 867 SQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDAL 926
           SQ  F RLHS+Q + + R++S QWK WAA  IQ AW  Y  +K+ +E + E + R +DAL
Sbjct: 601 SQ--FRRLHSKQLRHTFRYYSQQWKTWAACFIQAAWRRYI-KKKLEESLKEEENRLQDAL 660

Query: 927 AK-TVGASASFGAILYASIFISHLLQAVQQEHQQTAQPARVMTLPPP--PKPDEPDF 976
           AK   G+S S GA +YAS F +++L+ +++      +P     +PP    KP EPDF
Sbjct: 661 AKEACGSSPSLGATIYASRFAANILRTIRRS-GSVRKPRMPERMPPMLLQKPAEPDF 712

BLAST of Sgr025418 vs. ExPASy Swiss-Prot
Match: Q9LNJ0 (Probable cyclic nucleotide-gated ion channel 10 OS=Arabidopsis thaliana OX=3702 GN=CNGC10 PE=2 SV=2)

HSP 1 Score: 713.8 bits (1841), Expect = 2.7e-204
Identity = 362/644 (56.21%), Postives = 478/644 (74.22%), Query Frame = 0

Query: 347 KKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDGQHKCLTMDRPLMIIACVLRS 406
           K  ++PQ  FLQ WNKIF+ +CV+A+A+DPLFFYIP++D    CLT+D  L I A +LR+
Sbjct: 66  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 125

Query: 407 FIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAKRYLSSNFIIDILSILPLPQL 466
            ID FY++H++F+FRT ++ PS  VFG G+LV+D  AIA +YLSS FIID+LSILPLPQ+
Sbjct: 126 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 185

Query: 467 LVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLYREVTRTSGILTETAWSGAAF 526
           +VL ++P+   P+ L TKD++K +++ QY+PR+LR+YPLY EVTRTSGI+TETAW+GAA+
Sbjct: 186 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 245

Query: 527 NLLIYMLASHVVGAIWYLFSIEQQARCWLEAC-KRNNCASNYLYCGDHRRPAYPFIDRYC 586
           NL +YMLASHV GA+WYL S+E++ RCW EAC K   C   +LYC + R  +  F+   C
Sbjct: 246 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 305

Query: 587 PHKEPED---DNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWALRNVGSSGQNLQISNY 646
           P  +P D      FNFG++ +ALK  + E+ +F +KFFYCFWW LRN+ + GQNLQ S +
Sbjct: 306 PFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKF 365

Query: 647 LGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRRDAEHWMAHRMLPEDMR 706
           +GE++FA+ I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLPED+R
Sbjct: 366 VGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLR 425

Query: 707 QRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAM 766
           +RIRRY+QY+WQ  RGV+EE L+ NLPKDLRRDIKRHLCL LLKKVPLF  MD+QLLDA+
Sbjct: 426 KRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAV 485

Query: 767 CEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKDSYSDV-LKAGDFCGEE 826
           C+ L+PVL+TE SY+++EGDP+  MLF+M+G+L +  T GG+   ++ V LKA DFCGE+
Sbjct: 486 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGED 545

Query: 827 VVPWAMDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVASQFHFHRLHSQQFQLSVRF 886
           ++PWA+DP S+S  PISTRTV++LTEVEAFAL A +L+SVASQ  F RLHS+Q Q + RF
Sbjct: 546 LLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQ--FRRLHSKQLQHTFRF 605

Query: 887 HSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAK--------TVGASASFG 946
           +S QW+ W+   IQ AW  YC RK  K  + + + R R+ALA         TV +S S G
Sbjct: 606 YSVQWRTWSVSFIQAAWRRYCRRKLAK-SLRDEEDRLREALASQDKEHNAATVSSSLSLG 665

Query: 947 AILYASIFISHLLQAVQQEHQQTAQPARVMTLPPPPKPDEPDFT 977
             LYAS F S+ L  ++  H  +  P R      P KP EPDFT
Sbjct: 666 GALYASRFASNALHNLR--HNISNLPPRYTLPLLPQKPTEPDFT 704

BLAST of Sgr025418 vs. ExPASy Swiss-Prot
Match: Q9LD40 (Putative cyclic nucleotide-gated ion channel 13 OS=Arabidopsis thaliana OX=3702 GN=CNGC13 PE=3 SV=2)

HSP 1 Score: 704.1 bits (1816), Expect = 2.2e-201
Identity = 372/696 (53.45%), Postives = 498/696 (71.55%), Query Frame = 0

Query: 293 KYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSMRAVLRSKHTGDQSTKKPGFWKKFLDP 352
           +YG  +N++R S  +VL++++R L        S  +  + + +   +T +    K  ++P
Sbjct: 20  EYGYGRNKARPSLNTVLKNVRRGLKKPL----SFGSHNKKRDSNSSTTTQ----KNIINP 79

Query: 353 QQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDGQHKCLTMDRPLMIIACVLRSFIDIFY 412
           Q  FLQ WNKIF+ + VIA+A+DPLFFYIP++DG+  CL + R L I A VLR+FID FY
Sbjct: 80  QGSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFY 139

Query: 413 LLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAKRYLSSNFIIDILSILPLPQLLVLVIV 472
           ++H++F+FRT ++ PS  VFG G+LV+DP AIA +YLSS FIID+LSILPLPQL+VL ++
Sbjct: 140 IIHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVI 199

Query: 473 PAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLYREVTRTSGILTETAWSGAAFNLLIYM 532
           P    P+ L TKD++   +  QYIPR+LRIYPLY EVTRTSGI+TETAW+GAA+NL +YM
Sbjct: 200 PNVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYM 259

Query: 533 LASHVVGAIWYLFSIEQQARCWLEACKR--NNCASNYLYCGDHRRPAYPFIDRYCPHKEP 592
           LASHV GA+WYL S+E++ RCW EAC++    C   +LYC  +      F+   CP   P
Sbjct: 260 LASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINP 319

Query: 593 ED---DNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWALRNVGSSGQNLQISNYLGELL 652
           +D      FNFG++ +ALK  + E+ +F +KFFYCFWW LRN+ + GQNL  S ++GE++
Sbjct: 320 DDITNSTVFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEII 379

Query: 653 FAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRRDAEHWMAHRMLPEDMRQRIRR 712
           FAV I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLP+D+R+RIRR
Sbjct: 380 FAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRR 439

Query: 713 YDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLK 772
           Y+QYKWQ  RGV+EE L+ NLPKDLRRDIKRH CL LLKKVPLF  MD+QLLDA+C+ LK
Sbjct: 440 YEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLDAVCDKLK 499

Query: 773 PVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKDSYSDV-LKAGDFCGEEVVPWA 832
           PVL+TE SY ++EGDP++ MLF+M+GKL +  T GG+   ++ V LK  DFCGE+++ WA
Sbjct: 500 PVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLLTWA 559

Query: 833 MDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVASQFHFHRLHSQQFQLSVRFHSHQW 892
           +DP S+S  PISTRTV++LTEVEAFAL A++L+ VASQ  F RLHS+Q Q + RF+S QW
Sbjct: 560 LDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQ--FRRLHSKQLQHTFRFYSVQW 619

Query: 893 KVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAK---TVGASASFGAILYASIFIS 952
           + W A  IQ AW  +C RK  +  + E + RFR+A+ K      +S+S  A LYAS F S
Sbjct: 620 RTWGASFIQAAWRRHCRRKLAR-SLTEEEDRFRNAITKRERNAASSSSLVATLYASRFAS 679

Query: 953 HLLQAVQQEHQQTAQPARVMTLP-PPPKPDEPDFTI 978
           + L+ +           R   LP  PPKP EPDF++
Sbjct: 680 NALRNL-----------RTNNLPLLPPKPSEPDFSL 693

BLAST of Sgr025418 vs. ExPASy Swiss-Prot
Match: Q9SKD7 (Probable cyclic nucleotide-gated ion channel 3 OS=Arabidopsis thaliana OX=3702 GN=CNGC3 PE=2 SV=2)

HSP 1 Score: 656.8 bits (1693), Expect = 4.0e-187
Identity = 351/716 (49.02%), Postives = 482/716 (67.32%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M+ Q+ K  RF        +  +F  K       +R S  SV+++++R      E  R+ 
Sbjct: 2   MNPQRNKFVRF------NGNDDEFSTK------TTRPSVSSVMKTVRRSFEKGSEKIRTF 61

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
           +  L      ++   K     + ++P   +LQ WNKIF+L  V+A+A DPLFFYIP +  
Sbjct: 62  KRPLSVHSNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKP 121

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           +  CL +D+ L  IACV R+FID FY++HM+F+F TGF+ PS   FG G+L E    IA 
Sbjct: 122 ERFCLNLDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIAL 181

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL S F+ID+LSILP+PQ++VL IVP  + P  L  K+ +K  +  QY+PR+ RIYPL+
Sbjct: 182 RYLGSYFLIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLF 241

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEAC-KRNNCAS 566
           +EVTRTSG++TETAW+GAA NL +YMLASHV G+ WYL SIE++ RCW EAC K  NC  
Sbjct: 242 KEVTRTSGLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTH 301

Query: 567 NYLYCGDHRRPAYPFIDRYCPHKEPED---DNSFNFGMYIEALKFHLTETTNFRRKFFYC 626
            YLYC         F++  CP  +PE+      FNFG++ +AL+  + E+ +F +KFFYC
Sbjct: 302 AYLYCSPTGEDNRLFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYC 361

Query: 627 FWWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMR 686
           FWW LRN+ + GQNL+ S + GE++FA+ I I GLVLFALLI N+QKYLQSTTV++E+MR
Sbjct: 362 FWWGLRNLSALGQNLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMR 421

Query: 687 INRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCL 746
           + RRDAE WM+HRMLP+D+R+RIR+Y+QYKWQ  +GV+EE L+S+LPKDLR+DIKRHLCL
Sbjct: 422 VKRRDAEQWMSHRMLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCL 481

Query: 747 ALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYG 806
            LLKKVP F +MD +LLDA+C  LK VL+TEKSYI++EG+P++ MLFIM+G L +  TYG
Sbjct: 482 KLLKKVPWFQAMDDRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYG 541

Query: 807 GKKDSYSDV-LKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVA 866
           G+   ++ V L AGDFCG +++ WA+DP S+  PIS+RTV++LTEVE F L A++L+ VA
Sbjct: 542 GRTGFFNSVDLVAGDFCG-DLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVA 601

Query: 867 SQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDAL 926
           +Q  + RLHS+Q +   RF+S QW+ WAA  IQ AW  +C RK  K  + E +G+  + L
Sbjct: 602 TQ--YRRLHSKQLRHMFRFYSVQWQTWAACFIQAAWKRHCRRKLSK-ALREEEGKLHNTL 661

Query: 927 AK--TVGASASFGAILYASIFISHLLQAVQQEHQQTAQPARVMTLPPPPKPDEPDF 976
               + G   + GA +YAS F SH L+ ++            M    P KP +P+F
Sbjct: 662 QNDDSGGNKLNLGAAIYASRFASHALRNLRANAAARNSRFPHMLTLLPQKPADPEF 701

BLAST of Sgr025418 vs. ExPASy Swiss-Prot
Match: Q8RWS9 (Probable cyclic nucleotide-gated ion channel 5 OS=Arabidopsis thaliana OX=3702 GN=CNGC5 PE=2 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.0e-179
Identity = 328/641 (51.17%), Postives = 441/641 (68.80%), Query Frame = 0

Query: 347 KKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDGQHKCLTMDRPLMIIACVLRS 406
           KK  DPQ  FL   NK+FV SC+++V VDP FFY+PVI+ + KCL +DR L I A  LR+
Sbjct: 86  KKIFDPQDKFLLYCNKLFVASCILSVFVDPFFFYLPVINAESKCLGIDRKLAITASTLRT 145

Query: 407 FIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAKRYLSSNFIIDILSILPLPQL 466
           FID+FYL HM  + RT ++ PS  VFG G+LV DPA IAKRYL   FIID LS+LPLPQ+
Sbjct: 146 FIDVFYLAHMALQLRTAYIAPSSRVFGRGELVIDPAQIAKRYLQRWFIIDFLSVLPLPQI 205

Query: 467 LVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLYREVTRTSGILTETAWSGAAF 526
           +V   + ++ G   L TK  +   VL+QYIPR LR+ PL  E+ RT+G+  ETAW+GAA+
Sbjct: 206 VVWRFLQSSNGSDVLATKQALLFIVLVQYIPRFLRVLPLTSELKRTAGVFAETAWAGAAY 265

Query: 527 NLLIYMLASHVVGAIWYLFSIEQQARCWLEAC-KRNNCASNYLYCGDHRRPAYPFIDR-- 586
            LL+YMLASH+VGA WYL ++E+   CW EAC    NC++++LYCG+     Y   +R  
Sbjct: 266 YLLLYMLASHIVGAFWYLLALERNDACWQEACIDAGNCSTDFLYCGNQNMDGYAVWNRAK 325

Query: 587 ------YCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWALRNVGSSGQNL 646
                  C     +++  F+FG+Y +AL   +  + NF  K+ YC WW L+N+ + GQ L
Sbjct: 326 ESVLKSKCRADLDDNNPPFDFGIYTQALSSGIVSSQNFIVKYCYCLWWGLQNLSTLGQGL 385

Query: 647 QISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRRDAEHWMAHRML 706
           + S Y  E++F++ +AI GL+LFALLI N+Q YLQS T+++E+MR+ RRD+E WM HRML
Sbjct: 386 ETSTYPMEIIFSISLAISGLILFALLIGNMQTYLQSLTIRLEEMRVKRRDSEQWMHHRML 445

Query: 707 PEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQ 766
           P+D+R+R+RRYDQYKW   RGV EE L+ NLPKDLRRDIKRHLCLAL+++VPLF SMD +
Sbjct: 446 PQDLRERVRRYDQYKWLETRGVDEEYLVQNLPKDLRRDIKRHLCLALVRRVPLFKSMDDK 505

Query: 767 LLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKDSYS-DVLKAGD 826
           LLDA+C  LKP LFTE +Y+++EGDP+D MLFI++G+L ++ T GG+   ++  +LK G+
Sbjct: 506 LLDAICMRLKPCLFTESTYLVREGDPVDEMLFIIRGRLESVTTDGGRSGFFNRSLLKEGE 565

Query: 827 FCGEEVVPWAMDPHS-TSLPISTRTVKSLTEVEAFALKANELQSVASQFHFHRLHSQQFQ 886
           FCGEE++ WA+DP S  +LP STRTVK+LTEVEAFAL + EL+ VASQ  F RLHS+Q Q
Sbjct: 566 FCGEELLTWALDPKSGVNLPSSTRTVKALTEVEAFALTSEELKFVASQ--FRRLHSRQVQ 625

Query: 887 LSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVGASASFGAIL 946
            + RF+SHQW+ WAA  IQ AW  YC+RK+ +E   E         + T G S S GA  
Sbjct: 626 HTFRFYSHQWRTWAACFIQAAWRRYCKRKKMEEAEAEAAA----VSSSTAGPSYSIGAAF 685

Query: 947 YASIFISHLLQAVQQEHQQTAQPARVMTLPPPPKPDEPDFT 977
            A+ F ++ L+ + +         ++  L    KP EPDFT
Sbjct: 686 LATKFAANALRTIHRNRN-----TKIRDLVKLQKPPEPDFT 715

BLAST of Sgr025418 vs. ExPASy TrEMBL
Match: A0A6J1D1B4 (cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016363 PE=4 SV=1)

HSP 1 Score: 1189.1 bits (3075), Expect = 0.0e+00
Identity = 605/720 (84.03%), Postives = 648/720 (90.00%), Query Frame = 0

Query: 263 MSKFMSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEG 322
           MSKFM S KPKS RF +P      TSD HV+YG YQNRSRLSP S+LESIQR++SSA EG
Sbjct: 1   MSKFMGSLKPKSLRFGYP------TSDIHVRYGGYQNRSRLSPASILESIQRKISSASEG 60

Query: 323 FRSMRAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIP 382
           FR M A L  KH  DQS KKP FWKK LDPQ+PFLQQWNKIFVLSCV+AVAVDPLFFYIP
Sbjct: 61  FRRMVANLGFKHEDDQSRKKPEFWKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFYIP 120

Query: 383 VIDGQHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPA 442
           V+D QH+CLTMDR LMIIACVLRSFIDIFYLLH+IFEFRTGF+ PSLPVFGSG+LVEDPA
Sbjct: 121 VMDRQHQCLTMDRLLMIIACVLRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVEDPA 180

Query: 443 AIAKRYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRI 502
           AIAKRYLSSNFIIDILSI+PLPQLL LVI+PAAKGPIPLKTK+F+K+AVLLQY+PRL RI
Sbjct: 181 AIAKRYLSSNFIIDILSIIPLPQLLALVIIPAAKGPIPLKTKNFMKVAVLLQYLPRLFRI 240

Query: 503 YPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNN 562
           YPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFS+EQ++RCW EAC++NN
Sbjct: 241 YPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSVEQESRCWHEACRKNN 300

Query: 563 CASNYLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLT-ETTNFRRKFFY 622
           C S YLYCGDH RPAY FI  YCPHKE EDD +FNFG+Y+EALKF LT +TTNF  KFFY
Sbjct: 301 CNSKYLYCGDHSRPAYGFIKEYCPHKESEDDKTFNFGIYLEALKFRLTDQTTNFHSKFFY 360

Query: 623 CFWWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQM 682
           CFWWALRNVGSSGQNLQISNYLGEL FAVFIAILGLVLFALLI+NIQKYLQSTTVK+EQM
Sbjct: 361 CFWWALRNVGSSGQNLQISNYLGELFFAVFIAILGLVLFALLISNIQKYLQSTTVKVEQM 420

Query: 683 RINRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLC 742
           RINRRDAEHWM HRMLPED+RQRIRRYDQYKWQLNRGV EEELISNLPKDLRRDIKRHLC
Sbjct: 421 RINRRDAEHWMVHRMLPEDLRQRIRRYDQYKWQLNRGVNEEELISNLPKDLRRDIKRHLC 480

Query: 743 LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTY 802
           LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKS ILQEGDP+D MLFIMKG LATI TY
Sbjct: 481 LALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSDILQEGDPVDMMLFIMKGNLATIITY 540

Query: 803 GGKKDSYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVA 862
           G K DS+SDVLKAGDFCGEE+VPWAMDP STSLPISTRTVK+LTEVEAFALKA ELQSV 
Sbjct: 541 GWKNDSHSDVLKAGDFCGEELVPWAMDPQSTSLPISTRTVKTLTEVEAFALKAKELQSVV 600

Query: 863 SQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDAL 922
           S F F R +SQQFQLSVRF+SHQWKVWAAYKIQEAWHDYCERK++      GD RF+ AL
Sbjct: 601 SLFRFQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRR----GGGDSRFQGAL 660

Query: 923 AKTVGASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDEPDFTILDL 981
           AKTVGASASFGA LYASIFISHLLQ VQ+ +HQQT+Q +RVMTLPPPPKP E DFTILDL
Sbjct: 661 AKTVGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPIERDFTILDL 710

BLAST of Sgr025418 vs. ExPASy TrEMBL
Match: A0A6J1D2C8 (LOW QUALITY PROTEIN: cyclic nucleotide-gated ion channel 1-like OS=Momordica charantia OX=3673 GN=LOC111016362 PE=4 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 598/725 (82.48%), Postives = 641/725 (88.41%), Query Frame = 0

Query: 261 ILMSKFMSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAY 320
           +LMSKFM S KPK  RF +P      TSD HVKYG YQNRS LSP S+LESIQRR SSA 
Sbjct: 1   MLMSKFMGSLKPKFFRFGYP------TSDIHVKYGGYQNRSTLSPASILESIQRRFSSAS 60

Query: 321 EGFRSMRAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFY 380
           EGFR M AVL  KH  DQ  KK    KK LDPQ+PFLQQWNKIFVLSCV+AVAVDPLFFY
Sbjct: 61  EGFRRMEAVLCFKHEYDQPRKKLELSKKILDPQKPFLQQWNKIFVLSCVLAVAVDPLFFY 120

Query: 381 IPVIDGQHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVED 440
           IPVID QH+CLTMDR LMIIAC+LRSFIDIFYLLH+IFEFRTGF+ PSLPVFGSG+LVED
Sbjct: 121 IPVIDRQHQCLTMDRLLMIIACILRSFIDIFYLLHIIFEFRTGFLPPSLPVFGSGELVED 180

Query: 441 PAAIAKRYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLL 500
           PAAIAKRYLSSNFIIDILSI+PLPQLLVLVI+PAAKGPI L+TKDF+K+AVLLQYIPRLL
Sbjct: 181 PAAIAKRYLSSNFIIDILSIIPLPQLLVLVIIPAAKGPIALRTKDFVKVAVLLQYIPRLL 240

Query: 501 RIYPLYREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKR 560
           RIY LYREVTRTSGILTE AWSGAAFNLLIYMLASHVVGAIWYLFS+EQ+ RCWLEAC+ 
Sbjct: 241 RIYLLYREVTRTSGILTERAWSGAAFNLLIYMLASHVVGAIWYLFSVEQEERCWLEACRT 300

Query: 561 NNCASNYLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLT-ETTNFRRKF 620
            NC S  L CGD  R AY  I +YC HK+ EDD +FNFGMY+EALKFHLT +TTNFR KF
Sbjct: 301 RNCNSKDLCCGDPSRAAY-IIKQYCSHKKSEDDKTFNFGMYLEALKFHLTDQTTNFRDKF 360

Query: 621 FYCFWWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIE 680
           FYCFWWALRNVGS GQNLQISNYLGE+LFAVFIAILGL+LFALLI+NIQKYLQSTTVK+E
Sbjct: 361 FYCFWWALRNVGSCGQNLQISNYLGEVLFAVFIAILGLLLFALLISNIQKYLQSTTVKVE 420

Query: 681 QMRINRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRH 740
           QMRI+RRDAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGV+EEELISNLPKDLRRDI RH
Sbjct: 421 QMRISRRDAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVREEELISNLPKDLRRDINRH 480

Query: 741 LCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIF 800
           LCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTE S ILQEGDPID MLFIMKG LATI 
Sbjct: 481 LCLALLKKVPLFSSMDKQLLDAMCEYLKPVLFTENSNILQEGDPIDMMLFIMKGNLATII 540

Query: 801 TYGGKKDSYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQS 860
           TYG K DS+SDVLKAGDFCGEE+VPWAMDP STSLPI TRTVK+LTEVEAFALKA ELQS
Sbjct: 541 TYGWKNDSHSDVLKAGDFCGEELVPWAMDPQSTSLPIXTRTVKTLTEVEAFALKAKELQS 600

Query: 861 VASQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRD 920
           V SQFHF R +SQQFQLSVRF+SHQWKVWAAYKIQEAWHDYCERK++      GDGRF+D
Sbjct: 601 VVSQFHFQRFNSQQFQLSVRFYSHQWKVWAAYKIQEAWHDYCERKRR----GGGDGRFQD 660

Query: 921 ALAKTVGASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKP---DEPDF 980
           ALAKT GASASFGA LYASIFISHLLQ VQ+ +HQQT+Q +RVMTLPPPPKP   D+ DF
Sbjct: 661 ALAKTAGASASFGATLYASIFISHLLQVVQRDQHQQTSQLSRVMTLPPPPKPLEQDKRDF 714

BLAST of Sgr025418 vs. ExPASy TrEMBL
Match: A0A6J1IV06 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479553 PE=4 SV=1)

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 568/719 (79.00%), Postives = 629/719 (87.48%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M SQ PKSARF HP      TSDFH KYG +QNRSRLSP S+LESI+R+ SSAY+  R +
Sbjct: 1   MGSQIPKSARFGHP------TSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRI 60

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
              L  KH GDQS KKP FWKK LDPQ+PFLQQWNKIFVLS VIAVAVDPLFFYIPVIDG
Sbjct: 61  ITHLSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDG 120

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           + +CLT+DR LMIIACVLRS +D+FY+LHMIFEFRTGF+ PSLPVFGSG+L+EDPAAIAK
Sbjct: 121 ERQCLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAK 180

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL SNF+IDILS+LPLPQLLVL I+PAAKGPIPLKTKD +KIAVLLQY+PRLLRIYPLY
Sbjct: 181 RYLLSNFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLY 240

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNNCASN 566
           REVTRT GILTETAWSGAAFNLLIYM  SHVVGAIWYL S+E+QA+CW+EAC+++ C  N
Sbjct: 241 REVTRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDN 300

Query: 567 YLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWA 626
           +LYCG+ RR   P+ID+YC  KEPED  +FNFGMY+EALKF LT TT+FRRKFFY FWWA
Sbjct: 301 FLYCGNQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWA 360

Query: 627 LRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRR 686
           LRNV SSGQNLQ+SNY+GE+ FAVFIAILGLVLFALLI+NIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRR 420

Query: 687 DAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 746
           DAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLK
Sbjct: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLK 480

Query: 747 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKD 806
           KVPLFS MDKQLLDAMCEYLKPVLFTEKS+ILQEGDPID MLFIMKGKLATI TYG K D
Sbjct: 481 KVPLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKND 540

Query: 807 SYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVASQFHF 866
           ++S VL AGDFCGEE+V WA DP STSLPIS+RTVKSLTEVEAFALKANEL+SV SQFHF
Sbjct: 541 TFSAVLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHF 600

Query: 867 HRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVG 926
            RL+S+QFQLSVRF+SHQW VWAAYKIQEAW +Y ERK++  GV  GDGRF+ ALAKT G
Sbjct: 601 QRLNSKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRR--GV-RGDGRFQAALAKTAG 660

Query: 927 ASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDE----PDFTILDL 981
           AS  FGA LYASIFISHLLQ VQ+ +H++T Q  RVMTLPPPPKPD+    P+ TIL+L
Sbjct: 661 ASVGFGATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 710

BLAST of Sgr025418 vs. ExPASy TrEMBL
Match: A0A6J1EF20 (cyclic nucleotide-gated ion channel 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111431950 PE=4 SV=1)

HSP 1 Score: 1114.8 bits (2882), Expect = 0.0e+00
Identity = 563/718 (78.41%), Postives = 626/718 (87.19%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M SQ PKS RF HP      TSDFH KYG YQN SRLSP S+LESI+R+  SAY+ FR +
Sbjct: 1   MGSQIPKSTRFGHP------TSDFHDKYGSYQNGSRLSPGSILESIERKFLSAYKSFRRI 60

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
              L  KH GDQS KKP FWKK LDPQ+PFLQQWNKIFVLS VIAVAVDPLFFYIPVI+G
Sbjct: 61  ITHLSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVING 120

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           + +CLT+DR LMIIACVLRS +D+FY+LHMIFEFRTGF+ PSLPVFGSG+L+EDPAAIAK
Sbjct: 121 ERQCLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAK 180

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL SNF+IDILS+LPLPQLLVL I+PAA+GP+PLKTKD +KIAVLLQY+PRLLRIYPLY
Sbjct: 181 RYLFSNFLIDILSVLPLPQLLVLAIIPAARGPVPLKTKDLVKIAVLLQYVPRLLRIYPLY 240

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNNCASN 566
           REVTRT GILTETAWSGAAFNLLIYM  SHVVGAIWYL S+E+QA+CW+EAC+++ C  N
Sbjct: 241 REVTRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDN 300

Query: 567 YLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWA 626
           +LYCG+ RR   P+ID+YC  KE ED  +FNFGMY+EALKF LT TT+FRRKFFY FWWA
Sbjct: 301 FLYCGNQRRQIQPYIDQYCSPKESEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWA 360

Query: 627 LRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRR 686
           LRNV SSGQNLQ+SNY+GE+ FAVFIAILGLVLFALLI+NIQKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVSSSGQNLQVSNYMGEVFFAVFIAILGLVLFALLISNIQKYLQSATVKVEQMRINRR 420

Query: 687 DAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 746
           DAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLK
Sbjct: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLK 480

Query: 747 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKD 806
           KVPLFS MDKQLLDAMCEYLKPVLFTEKS+ILQEGDPID +LFIMKGKLATI TYG K D
Sbjct: 481 KVPLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMVLFIMKGKLATIITYGWKND 540

Query: 807 SYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVASQFHF 866
           + S VL AGDFCGEE+V WAMDP STSLPIS+RTVKSLTEVEAFALKANEL+SV SQFHF
Sbjct: 541 TLSGVLNAGDFCGEELVQWAMDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHF 600

Query: 867 HRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVG 926
             L+S+QFQLSVRF+SHQW VWAAYK+QEAW DY ERK++  GV  GDGRF+DALAKT G
Sbjct: 601 QHLNSKQFQLSVRFYSHQWNVWAAYKVQEAWQDYRERKRR--GV-RGDGRFQDALAKTAG 660

Query: 927 ASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDE----PDFTILD 980
           AS  FGA LYASIFISHLLQ VQ+ +HQ+T Q  +VMTLPPPPKPD+    P+FTIL+
Sbjct: 661 ASVGFGATLYASIFISHLLQVVQRDQHQETTQLTQVMTLPPPPKPDDEPDSPNFTILN 709

BLAST of Sgr025418 vs. ExPASy TrEMBL
Match: A0A6J1IV11 (cyclic nucleotide-gated ion channel 1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111479553 PE=4 SV=1)

HSP 1 Score: 1060.1 bits (2740), Expect = 5.7e-306
Identity = 543/719 (75.52%), Postives = 600/719 (83.45%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M SQ PKSARF HP      TSDFH KYG +QNRSRLSP S+LESI+R+ SSAY+  R +
Sbjct: 1   MGSQIPKSARFGHP------TSDFHDKYGSHQNRSRLSPGSILESIERKFSSAYKSVRRI 60

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
              L  KH GDQS KKP FWKK LDPQ+PFLQQWNKIFVLS VIAVAVDPLFFYIPVIDG
Sbjct: 61  ITHLSFKHPGDQSVKKPKFWKKILDPQKPFLQQWNKIFVLSSVIAVAVDPLFFYIPVIDG 120

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           + +CLT+DR LMIIACVLRS +D+FY+LHMIFEFRTGF+ PSLPVFGSG+L+EDPAAIAK
Sbjct: 121 ERQCLTIDRQLMIIACVLRSVMDLFYVLHMIFEFRTGFLPPSLPVFGSGELIEDPAAIAK 180

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL SNF+IDILS+LPLPQLLVL I+PAAKGPIPLKTKD +KIAVLLQY+PRLLRIYPLY
Sbjct: 181 RYLLSNFLIDILSVLPLPQLLVLAIIPAAKGPIPLKTKDVVKIAVLLQYVPRLLRIYPLY 240

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNNCASN 566
           REVTRT GILTETAWSGAAFNLLIYM  SHVVGAIWYL S+E+QA+CW+EAC+++ C  N
Sbjct: 241 REVTRTVGILTETAWSGAAFNLLIYMQVSHVVGAIWYLLSVERQAKCWVEACRKDKCKDN 300

Query: 567 YLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWA 626
           +LYCG+ RR   P+ID+YC  KEPED  +FNFGMY+EALKF LT TT+FRRKFFY FWWA
Sbjct: 301 FLYCGNQRRQIQPYIDQYCSPKEPEDGKTFNFGMYVEALKFQLTSTTSFRRKFFYSFWWA 360

Query: 627 LRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRR 686
           LRNV SSGQNL                              QKYLQS TVK+EQMRINRR
Sbjct: 361 LRNVSSSGQNL------------------------------QKYLQSATVKVEQMRINRR 420

Query: 687 DAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLK 746
           DAEHWMAHRMLPED+RQRIRRYDQYKWQLNRGVKEEELI NLPKDLRRDIKRHLC ALLK
Sbjct: 421 DAEHWMAHRMLPEDLRQRIRRYDQYKWQLNRGVKEEELIGNLPKDLRRDIKRHLCYALLK 480

Query: 747 KVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKD 806
           KVPLFS MDKQLLDAMCEYLKPVLFTEKS+ILQEGDPID MLFIMKGKLATI TYG K D
Sbjct: 481 KVPLFSGMDKQLLDAMCEYLKPVLFTEKSFILQEGDPIDMMLFIMKGKLATIITYGWKND 540

Query: 807 SYSDVLKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVASQFHF 866
           ++S VL AGDFCGEE+V WA DP STSLPIS+RTVKSLTEVEAFALKANEL+SV SQFHF
Sbjct: 541 TFSAVLNAGDFCGEELVQWARDPTSTSLPISSRTVKSLTEVEAFALKANELKSVTSQFHF 600

Query: 867 HRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAKTVG 926
            RL+S+QFQLSVRF+SHQW VWAAYKIQEAW +Y ERK++  GV  GDGRF+ ALAKT G
Sbjct: 601 QRLNSKQFQLSVRFYSHQWNVWAAYKIQEAWQEYRERKRR--GV-RGDGRFQAALAKTAG 660

Query: 927 ASASFGAILYASIFISHLLQAVQQ-EHQQTAQPARVMTLPPPPKPDE----PDFTILDL 981
           AS  FGA LYASIFISHLLQ VQ+ +H++T Q  RVMTLPPPPKPD+    P+ TIL+L
Sbjct: 661 ASVGFGATLYASIFISHLLQVVQRDQHRETVQLTRVMTLPPPPKPDDEPDRPNVTILNL 680

BLAST of Sgr025418 vs. TAIR 10
Match: AT5G53130.1 (cyclic nucleotide gated channel 1 )

HSP 1 Score: 735.3 bits (1897), Expect = 6.2e-212
Identity = 388/717 (54.11%), Postives = 519/717 (72.38%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTS-DFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRS 326
           M+ ++ K  RF    + + S+  ++  K  +     + +  S+ +   R   S+    + 
Sbjct: 1   MNFRQEKFVRFQDWKSDKTSSDVEYSGKNEIQTGIFQRTISSISDKFYRSFESSSARIKL 60

Query: 327 MRAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVID 386
            +   +S    +  +K  G   K LDPQ PFLQ+WNKIFVL+C+IAV++DPLFFY+P+ID
Sbjct: 61  FKRSYKSYSFKEAVSKGIGSTHKILDPQGPFLQRWNKIFVLACIIAVSLDPLFFYVPIID 120

Query: 387 GQHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIA 446
              KCL +D+ + I A VLRSF D+FY+LH+IF+FRTGF+ PS  VFG G LVED   IA
Sbjct: 121 DAKKCLGIDKKMEITASVLRSFTDVFYVLHIIFQFRTGFIAPSSRVFGRGVLVEDKREIA 180

Query: 447 KRYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPL 506
           KRYLSS+FIIDIL++LPLPQ+++L+I+P  +G   L TK+ +K  V  QYIPR +RIYPL
Sbjct: 181 KRYLSSHFIIDILAVLPLPQMVILIIIPHMRGSSSLNTKNMLKFIVFFQYIPRFIRIYPL 240

Query: 507 YREVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEACKRNN--C 566
           Y+EVTRTSGILTETAW+GAAFNL +YMLASHV GA WYLFSIE++  CW +AC+RNN  C
Sbjct: 241 YKEVTRTSGILTETAWAGAAFNLFLYMLASHVFGAFWYLFSIERETVCWKQACERNNPPC 300

Query: 567 ASNYLYCGDHRRPAYPFIDRYCPHKEPEDDNSFNFGMYIEALKFHLTETTNFRRKFFYCF 626
            S  LYC         F++  CP + P +   F+FG++++AL+  + E+ +F +KFFYCF
Sbjct: 301 ISKLLYCDPETAGGNAFLNESCPIQTP-NTTLFDFGIFLDALQSGVVESQDFPQKFFYCF 360

Query: 627 WWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRI 686
           WW L+N+ S GQNL+ S Y+ E+ FAVFI+I GLVLF+ LI N+Q YLQSTT ++E+MR+
Sbjct: 361 WWGLQNLSSLGQNLKTSTYIWEICFAVFISIAGLVLFSFLIGNMQTYLQSTTTRLEEMRV 420

Query: 687 NRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLA 746
            RRDAE WM+HR+LPE++R+RIRRY+QYKWQ  RGV EE L+SNLPKDLRRDIKRHLCLA
Sbjct: 421 KRRDAEQWMSHRLLPENLRKRIRRYEQYKWQETRGVDEENLLSNLPKDLRRDIKRHLCLA 480

Query: 747 LLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGG 806
           LL +VP+F  MD+QLLDA+C+ L+PVL+TE+SYI++EGDP+D MLFIM+GKL TI T GG
Sbjct: 481 LLMRVPMFEKMDEQLLDALCDRLQPVLYTEESYIVREGDPVDEMLFIMRGKLLTITTNGG 540

Query: 807 KKDSY-SDVLKAGDFCGEEVVPWAMDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVA 866
           +     S+ L AGDFCGEE++ WA+DPHS+S LPISTRTV++L EVEAFALKA++L+ VA
Sbjct: 541 RTGFLNSEYLGAGDFCGEELLTWALDPHSSSNLPISTRTVRALMEVEAFALKADDLKFVA 600

Query: 867 SQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDAL 926
           SQ  F RLHS+Q + + R++S QWK WAA  IQ AW  Y  +K+ +E + E + R +DAL
Sbjct: 601 SQ--FRRLHSKQLRHTFRYYSQQWKTWAACFIQAAWRRYI-KKKLEESLKEEENRLQDAL 660

Query: 927 AK-TVGASASFGAILYASIFISHLLQAVQQEHQQTAQPARVMTLPPP--PKPDEPDF 976
           AK   G+S S GA +YAS F +++L+ +++      +P     +PP    KP EPDF
Sbjct: 661 AKEACGSSPSLGATIYASRFAANILRTIRRS-GSVRKPRMPERMPPMLLQKPAEPDF 712

BLAST of Sgr025418 vs. TAIR 10
Match: AT1G01340.1 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 713.8 bits (1841), Expect = 1.9e-205
Identity = 362/644 (56.21%), Postives = 478/644 (74.22%), Query Frame = 0

Query: 347 KKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDGQHKCLTMDRPLMIIACVLRS 406
           K  ++PQ  FLQ WNKIF+ +CV+A+A+DPLFFYIP++D    CLT+D  L I A +LR+
Sbjct: 61  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 120

Query: 407 FIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAKRYLSSNFIIDILSILPLPQL 466
            ID FY++H++F+FRT ++ PS  VFG G+LV+D  AIA +YLSS FIID+LSILPLPQ+
Sbjct: 121 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 180

Query: 467 LVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLYREVTRTSGILTETAWSGAAF 526
           +VL ++P+   P+ L TKD++K +++ QY+PR+LR+YPLY EVTRTSGI+TETAW+GAA+
Sbjct: 181 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 240

Query: 527 NLLIYMLASHVVGAIWYLFSIEQQARCWLEAC-KRNNCASNYLYCGDHRRPAYPFIDRYC 586
           NL +YMLASHV GA+WYL S+E++ RCW EAC K   C   +LYC + R  +  F+   C
Sbjct: 241 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 300

Query: 587 PHKEPED---DNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWALRNVGSSGQNLQISNY 646
           P  +P D      FNFG++ +ALK  + E+ +F +KFFYCFWW LRN+ + GQNLQ S +
Sbjct: 301 PFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKF 360

Query: 647 LGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRRDAEHWMAHRMLPEDMR 706
           +GE++FA+ I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLPED+R
Sbjct: 361 VGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLR 420

Query: 707 QRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAM 766
           +RIRRY+QY+WQ  RGV+EE L+ NLPKDLRRDIKRHLCL LLKKVPLF  MD+QLLDA+
Sbjct: 421 KRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAV 480

Query: 767 CEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKDSYSDV-LKAGDFCGEE 826
           C+ L+PVL+TE SY+++EGDP+  MLF+M+G+L +  T GG+   ++ V LKA DFCGE+
Sbjct: 481 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGED 540

Query: 827 VVPWAMDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVASQFHFHRLHSQQFQLSVRF 886
           ++PWA+DP S+S  PISTRTV++LTEVEAFAL A +L+SVASQ  F RLHS+Q Q + RF
Sbjct: 541 LLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQ--FRRLHSKQLQHTFRF 600

Query: 887 HSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAK--------TVGASASFG 946
           +S QW+ W+   IQ AW  YC RK  K  + + + R R+ALA         TV +S S G
Sbjct: 601 YSVQWRTWSVSFIQAAWRRYCRRKLAK-SLRDEEDRLREALASQDKEHNAATVSSSLSLG 660

Query: 947 AILYASIFISHLLQAVQQEHQQTAQPARVMTLPPPPKPDEPDFT 977
             LYAS F S+ L  ++  H  +  P R      P KP EPDFT
Sbjct: 661 GALYASRFASNALHNLR--HNISNLPPRYTLPLLPQKPTEPDFT 699

BLAST of Sgr025418 vs. TAIR 10
Match: AT1G01340.2 (cyclic nucleotide gated channel 10 )

HSP 1 Score: 713.8 bits (1841), Expect = 1.9e-205
Identity = 362/644 (56.21%), Postives = 478/644 (74.22%), Query Frame = 0

Query: 347 KKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDGQHKCLTMDRPLMIIACVLRS 406
           K  ++PQ  FLQ WNKIF+ +CV+A+A+DPLFFYIP++D    CLT+D  L I A +LR+
Sbjct: 66  KNIINPQDSFLQNWNKIFLFACVVALAIDPLFFYIPIVDSARHCLTLDSKLEIAASLLRT 125

Query: 407 FIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAKRYLSSNFIIDILSILPLPQL 466
            ID FY++H++F+FRT ++ PS  VFG G+LV+D  AIA +YLSS FIID+LSILPLPQ+
Sbjct: 126 LIDAFYIIHIVFQFRTAYIAPSSRVFGRGELVDDAKAIALKYLSSYFIIDLLSILPLPQI 185

Query: 467 LVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLYREVTRTSGILTETAWSGAAF 526
           +VL ++P+   P+ L TKD++K +++ QY+PR+LR+YPLY EVTRTSGI+TETAW+GAA+
Sbjct: 186 VVLAVIPSVNQPVSLLTKDYLKFSIIAQYVPRILRMYPLYTEVTRTSGIVTETAWAGAAW 245

Query: 527 NLLIYMLASHVVGAIWYLFSIEQQARCWLEAC-KRNNCASNYLYCGDHRRPAYPFIDRYC 586
           NL +YMLASHV GA+WYL S+E++ RCW EAC K   C   +LYC + R  +  F+   C
Sbjct: 246 NLSLYMLASHVFGALWYLISVEREDRCWQEACEKTKGCNMKFLYCENDRNVSNNFLTTSC 305

Query: 587 PHKEPED---DNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWALRNVGSSGQNLQISNY 646
           P  +P D      FNFG++ +ALK  + E+ +F +KFFYCFWW LRN+ + GQNLQ S +
Sbjct: 306 PFLDPGDITNSTIFNFGIFTDALKSGVVESHDFWKKFFYCFWWGLRNLSALGQNLQTSKF 365

Query: 647 LGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRRDAEHWMAHRMLPEDMR 706
           +GE++FA+ I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLPED+R
Sbjct: 366 VGEIIFAISICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPEDLR 425

Query: 707 QRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAM 766
           +RIRRY+QY+WQ  RGV+EE L+ NLPKDLRRDIKRHLCL LLKKVPLF  MD+QLLDA+
Sbjct: 426 KRIRRYEQYRWQETRGVEEETLLRNLPKDLRRDIKRHLCLDLLKKVPLFEIMDEQLLDAV 485

Query: 767 CEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKDSYSDV-LKAGDFCGEE 826
           C+ L+PVL+TE SY+++EGDP+  MLF+M+G+L +  T GG+   ++ V LKA DFCGE+
Sbjct: 486 CDRLRPVLYTENSYVIREGDPVGEMLFVMRGRLVSATTNGGRSGFFNAVNLKASDFCGED 545

Query: 827 VVPWAMDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVASQFHFHRLHSQQFQLSVRF 886
           ++PWA+DP S+S  PISTRTV++LTEVEAFAL A +L+SVASQ  F RLHS+Q Q + RF
Sbjct: 546 LLPWALDPQSSSHFPISTRTVQALTEVEAFALTAEDLKSVASQ--FRRLHSKQLQHTFRF 605

Query: 887 HSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAK--------TVGASASFG 946
           +S QW+ W+   IQ AW  YC RK  K  + + + R R+ALA         TV +S S G
Sbjct: 606 YSVQWRTWSVSFIQAAWRRYCRRKLAK-SLRDEEDRLREALASQDKEHNAATVSSSLSLG 665

Query: 947 AILYASIFISHLLQAVQQEHQQTAQPARVMTLPPPPKPDEPDFT 977
             LYAS F S+ L  ++  H  +  P R      P KP EPDFT
Sbjct: 666 GALYASRFASNALHNLR--HNISNLPPRYTLPLLPQKPTEPDFT 704

BLAST of Sgr025418 vs. TAIR 10
Match: AT4G01010.1 (cyclic nucleotide-gated channel 13 )

HSP 1 Score: 704.1 bits (1816), Expect = 1.5e-202
Identity = 372/696 (53.45%), Postives = 498/696 (71.55%), Query Frame = 0

Query: 293 KYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSMRAVLRSKHTGDQSTKKPGFWKKFLDP 352
           +YG  +N++R S  +VL++++R L        S  +  + + +   +T +    K  ++P
Sbjct: 20  EYGYGRNKARPSLNTVLKNVRRGLKKPL----SFGSHNKKRDSNSSTTTQ----KNIINP 79

Query: 353 QQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDGQHKCLTMDRPLMIIACVLRSFIDIFY 412
           Q  FLQ WNKIF+ + VIA+A+DPLFFYIP++DG+  CL + R L I A VLR+FID FY
Sbjct: 80  QGSFLQNWNKIFLFASVIALAIDPLFFYIPIVDGERHCLNLHRNLEIAASVLRTFIDAFY 139

Query: 413 LLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAKRYLSSNFIIDILSILPLPQLLVLVIV 472
           ++H++F+FRT ++ PS  VFG G+LV+DP AIA +YLSS FIID+LSILPLPQL+VL ++
Sbjct: 140 IIHIVFQFRTAYISPSSRVFGRGELVDDPKAIAIKYLSSYFIIDLLSILPLPQLVVLAVI 199

Query: 473 PAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLYREVTRTSGILTETAWSGAAFNLLIYM 532
           P    P+ L TKD++   +  QYIPR+LRIYPLY EVTRTSGI+TETAW+GAA+NL +YM
Sbjct: 200 PNVNKPVSLITKDYLITVIFTQYIPRILRIYPLYTEVTRTSGIVTETAWAGAAWNLSLYM 259

Query: 533 LASHVVGAIWYLFSIEQQARCWLEACKR--NNCASNYLYCGDHRRPAYPFIDRYCPHKEP 592
           LASHV GA+WYL S+E++ RCW EAC++    C   +LYC  +      F+   CP   P
Sbjct: 260 LASHVFGALWYLISVEREDRCWREACEKIPEVCNFRFLYCDGNSSVRNDFLTTSCPFINP 319

Query: 593 ED---DNSFNFGMYIEALKFHLTETTNFRRKFFYCFWWALRNVGSSGQNLQISNYLGELL 652
           +D      FNFG++ +ALK  + E+ +F +KFFYCFWW LRN+ + GQNL  S ++GE++
Sbjct: 320 DDITNSTVFNFGIFTDALKSGIVESDDFWKKFFYCFWWGLRNLSALGQNLNTSKFVGEII 379

Query: 653 FAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMRINRRDAEHWMAHRMLPEDMRQRIRR 712
           FAV I I GLVLFALLI N+QKYL+STTV+ E+MR+ +RDAE WM+HRMLP+D+R+RIRR
Sbjct: 380 FAVSICISGLVLFALLIGNMQKYLESTTVREEEMRVRKRDAEQWMSHRMLPDDLRKRIRR 439

Query: 713 YDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCLALLKKVPLFSSMDKQLLDAMCEYLK 772
           Y+QYKWQ  RGV+EE L+ NLPKDLRRDIKRH CL LLKKVPLF  MD+QLLDA+C+ LK
Sbjct: 440 YEQYKWQETRGVEEENLLRNLPKDLRRDIKRHFCLDLLKKVPLFEIMDEQLLDAVCDKLK 499

Query: 773 PVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYGGKKDSYSDV-LKAGDFCGEEVVPWA 832
           PVL+TE SY ++EGDP++ MLF+M+GKL +  T GG+   ++ V LK  DFCGE+++ WA
Sbjct: 500 PVLYTENSYAIREGDPVEEMLFVMRGKLMSATTNGGRTGFFNAVYLKPSDFCGEDLLTWA 559

Query: 833 MDPHSTS-LPISTRTVKSLTEVEAFALKANELQSVASQFHFHRLHSQQFQLSVRFHSHQW 892
           +DP S+S  PISTRTV++LTEVEAFAL A++L+ VASQ  F RLHS+Q Q + RF+S QW
Sbjct: 560 LDPQSSSHFPISTRTVQALTEVEAFALAADDLKLVASQ--FRRLHSKQLQHTFRFYSVQW 619

Query: 893 KVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDALAK---TVGASASFGAILYASIFIS 952
           + W A  IQ AW  +C RK  +  + E + RFR+A+ K      +S+S  A LYAS F S
Sbjct: 620 RTWGASFIQAAWRRHCRRKLAR-SLTEEEDRFRNAITKRERNAASSSSLVATLYASRFAS 679

Query: 953 HLLQAVQQEHQQTAQPARVMTLP-PPPKPDEPDFTI 978
           + L+ +           R   LP  PPKP EPDF++
Sbjct: 680 NALRNL-----------RTNNLPLLPPKPSEPDFSL 693

BLAST of Sgr025418 vs. TAIR 10
Match: AT2G46430.1 (cyclic nucleotide gated channel 3 )

HSP 1 Score: 656.8 bits (1693), Expect = 2.8e-188
Identity = 351/716 (49.02%), Postives = 482/716 (67.32%), Query Frame = 0

Query: 267 MSSQKPKSARFAHPTAGRASTSDFHVKYGVYQNRSRLSPESVLESIQRRLSSAYEGFRSM 326
           M+ Q+ K  RF        +  +F  K       +R S  SV+++++R      E  R+ 
Sbjct: 2   MNPQRNKFVRF------NGNDDEFSTK------TTRPSVSSVMKTVRRSFEKGSEKIRTF 61

Query: 327 RAVLRSKHTGDQSTKKPGFWKKFLDPQQPFLQQWNKIFVLSCVIAVAVDPLFFYIPVIDG 386
           +  L      ++   K     + ++P   +LQ WNKIF+L  V+A+A DPLFFYIP +  
Sbjct: 62  KRPLSVHSNKNKENNKKKKILRVMNPNDSYLQSWNKIFLLLSVVALAFDPLFFYIPYVKP 121

Query: 387 QHKCLTMDRPLMIIACVLRSFIDIFYLLHMIFEFRTGFVLPSLPVFGSGDLVEDPAAIAK 446
           +  CL +D+ L  IACV R+FID FY++HM+F+F TGF+ PS   FG G+L E    IA 
Sbjct: 122 ERFCLNLDKKLQTIACVFRTFIDAFYVVHMLFQFHTGFITPSSSGFGRGELNEKHKDIAL 181

Query: 447 RYLSSNFIIDILSILPLPQLLVLVIVPAAKGPIPLKTKDFIKIAVLLQYIPRLLRIYPLY 506
           RYL S F+ID+LSILP+PQ++VL IVP  + P  L  K+ +K  +  QY+PR+ RIYPL+
Sbjct: 182 RYLGSYFLIDLLSILPIPQVVVLAIVPRMRRPASLVAKELLKWVIFCQYVPRIARIYPLF 241

Query: 507 REVTRTSGILTETAWSGAAFNLLIYMLASHVVGAIWYLFSIEQQARCWLEAC-KRNNCAS 566
           +EVTRTSG++TETAW+GAA NL +YMLASHV G+ WYL SIE++ RCW EAC K  NC  
Sbjct: 242 KEVTRTSGLVTETAWAGAALNLFLYMLASHVFGSFWYLISIERKDRCWREACAKIQNCTH 301

Query: 567 NYLYCGDHRRPAYPFIDRYCPHKEPED---DNSFNFGMYIEALKFHLTETTNFRRKFFYC 626
            YLYC         F++  CP  +PE+      FNFG++ +AL+  + E+ +F +KFFYC
Sbjct: 302 AYLYCSPTGEDNRLFLNGSCPLIDPEEITNSTVFNFGIFADALQSGVVESRDFPKKFFYC 361

Query: 627 FWWALRNVGSSGQNLQISNYLGELLFAVFIAILGLVLFALLITNIQKYLQSTTVKIEQMR 686
           FWW LRN+ + GQNL+ S + GE++FA+ I I GLVLFALLI N+QKYLQSTTV++E+MR
Sbjct: 362 FWWGLRNLSALGQNLKTSAFEGEIIFAIVICISGLVLFALLIGNMQKYLQSTTVRVEEMR 421

Query: 687 INRRDAEHWMAHRMLPEDMRQRIRRYDQYKWQLNRGVKEEELISNLPKDLRRDIKRHLCL 746
           + RRDAE WM+HRMLP+D+R+RIR+Y+QYKWQ  +GV+EE L+S+LPKDLR+DIKRHLCL
Sbjct: 422 VKRRDAEQWMSHRMLPDDLRKRIRKYEQYKWQETKGVEEEALLSSLPKDLRKDIKRHLCL 481

Query: 747 ALLKKVPLFSSMDKQLLDAMCEYLKPVLFTEKSYILQEGDPIDTMLFIMKGKLATIFTYG 806
            LLKKVP F +MD +LLDA+C  LK VL+TEKSYI++EG+P++ MLFIM+G L +  TYG
Sbjct: 482 KLLKKVPWFQAMDDRLLDALCARLKTVLYTEKSYIVREGEPVEDMLFIMRGNLISTTTYG 541

Query: 807 GKKDSYSDV-LKAGDFCGEEVVPWAMDPHSTSLPISTRTVKSLTEVEAFALKANELQSVA 866
           G+   ++ V L AGDFCG +++ WA+DP S+  PIS+RTV++LTEVE F L A++L+ VA
Sbjct: 542 GRTGFFNSVDLVAGDFCG-DLLTWALDPLSSQFPISSRTVQALTEVEGFLLSADDLKFVA 601

Query: 867 SQFHFHRLHSQQFQLSVRFHSHQWKVWAAYKIQEAWHDYCERKQKKEGVWEGDGRFRDAL 926
           +Q  + RLHS+Q +   RF+S QW+ WAA  IQ AW  +C RK  K  + E +G+  + L
Sbjct: 602 TQ--YRRLHSKQLRHMFRFYSVQWQTWAACFIQAAWKRHCRRKLSK-ALREEEGKLHNTL 661

Query: 927 AK--TVGASASFGAILYASIFISHLLQAVQQEHQQTAQPARVMTLPPPPKPDEPDF 976
               + G   + GA +YAS F SH L+ ++            M    P KP +P+F
Sbjct: 662 QNDDSGGNKLNLGAAIYASRFASHALRNLRANAAARNSRFPHMLTLLPQKPADPEF 701