Sgr025416 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025416 vs. NCBI nr
Match: XP_022147293.1 (anthocyanidin reductase ((2S)-flavan-3-ol-forming)-like [Momordica charantia])

HSP 1 Score: 553.5 bits (1425), Expect = 1.3e-153
Identity = 271/344 (78.78%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 4   SGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLF 63
           SGC S VCVTGGSGYVAS LVKKLLQKGHTVHATLRNLND+SKV LL + PNA+ +L+LF
Sbjct: 6   SGCGSRVCVTGGSGYVASLLVKKLLQKGHTVHATLRNLNDQSKVRLLNTLPNAAHRLVLF 65

Query: 64  KADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVR 123
           +ADIY+P GF PPI+GCDFVFHVATPL H+QGSQYKNRT+ASV AA+MIAKFCVESGTVR
Sbjct: 66  QADIYQPEGFRPPIQGCDFVFHVATPLHHTQGSQYKNRTDASVGAARMIAKFCVESGTVR 125

Query: 124 RLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLG 183
           RLIYTASV+SASPMKDDG+GFKDFMDETCWTPLNLSYP+ N   EGY+DSK+ TE+ LL 
Sbjct: 126 RLIYTASVVSASPMKDDGTGFKDFMDETCWTPLNLSYPFSNPTFEGYVDSKSATERELLE 185

Query: 184 FGE---SEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQF---TDKAPAFHALRFVE 243
           FG     E GGGLEVVTLACGLI GDTL    AETTIITFSQF    D  P +HALRF+E
Sbjct: 186 FGARQVQEGGGGLEVVTLACGLIVGDTLHCCAAETTIITFSQFIHQDDAQPFYHALRFLE 245

Query: 244 EVDGKVPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGV 303
           E+DGKVPLVHIHDV DAHIFCMEQSS+HGRFLCASS+LSSS+IA+YYRLHHP+LQQK+G 
Sbjct: 246 ELDGKVPLVHIHDVCDAHIFCMEQSSLHGRFLCASSFLSSSDIATYYRLHHPQLQQKYGN 305

Query: 304 SDDVPVRNIKWNSRKLIDKGFRYNYDGEMILEDAFECYDKKEIS 342
           SDDVP+RNIKWNSRKLI+KGF Y YDGEMILEDA EC   K IS
Sbjct: 306 SDDVPIRNIKWNSRKLIEKGFVYKYDGEMILEDALECCKNKAIS 349

BLAST of Sgr025416 vs. NCBI nr
Match: XP_038874896.1 (putative anthocyanidin reductase [Benincasa hispida])

HSP 1 Score: 494.6 bits (1272), Expect = 7.1e-136
Identity = 241/329 (73.25%), Postives = 277/329 (84.19%), Query Frame = 0

Query: 10  VCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKADIYE 69
           VCVTGGSGYVAS L+KKLL KGH VHAT+RNL+DESK G+LK  PNA+T L+LFKADIYE
Sbjct: 3   VCVTGGSGYVASSLIKKLLLKGHIVHATVRNLDDESKAGILKRLPNATTNLVLFKADIYE 62

Query: 70  PNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLIYTA 129
           P+ FE  IRGC  VFHVATPL H+ G+QY N TEASV AAK IAKFCVE GTVRRLIYTA
Sbjct: 63  PHQFEAAIRGCHIVFHVATPLHHTHGTQYINSTEASVMAAKKIAKFCVELGTVRRLIYTA 122

Query: 130 SVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLGFGESEE 189
           S +S SPMKDDGSGFKDF+DETCWTPLN S P+ N   E Y+DSKT TEK LL FGESEE
Sbjct: 123 STVSMSPMKDDGSGFKDFLDETCWTPLNFSRPFSNSTFEAYVDSKTVTEKELLKFGESEE 182

Query: 190 GGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLVHIH 249
            G LEVVTLACGLIAG+T+  S+  +TIITFSQFTD++ AF+ LR++EE+DGKVPLVHI 
Sbjct: 183 SGNLEVVTLACGLIAGNTVHPSSNASTIITFSQFTDESEAFNILRYLEEIDGKVPLVHID 242

Query: 250 DVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPVRNIKWN 309
           DV DAHIFCMEQSSIHGRFLCA+S+LSSS+IA+YYRLHHP+L+QK G  D+V  RNIK N
Sbjct: 243 DVCDAHIFCMEQSSIHGRFLCATSFLSSSDIANYYRLHHPQLRQKQGECDEVANRNIKMN 302

Query: 310 SRKLIDKGFRYNYDGEMILEDAFECYDKK 339
           S+KLI++GF Y YDG+MIL+DAF  Y K+
Sbjct: 303 SKKLIERGFIYKYDGDMILDDAFHGYKKQ 331

BLAST of Sgr025416 vs. NCBI nr
Match: XP_008437918.1 (PREDICTED: vestitone reductase-like [Cucumis melo] >KAA0048905.1 vestitone reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 484.2 bits (1245), Expect = 9.7e-133
Identity = 240/332 (72.29%), Postives = 271/332 (81.63%), Query Frame = 0

Query: 3   ASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLL 62
           +SGC   VCVTGGSGYVAS L+KKLL  GH VHATLRNL+DESK+G+LK  PNA+T L+L
Sbjct: 6   SSGC-LRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNLVL 65

Query: 63  FKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTV 122
           FKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAKMIAK CVE GTV
Sbjct: 66  FKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVELGTV 125

Query: 123 RRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLL 182
           RRLIYTASV+S SPMKDDGSGFKD +DE CWTPL LSYP+ N  + GY+DSKT TEK LL
Sbjct: 126 RRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATEKELL 185

Query: 183 GFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGK 242
            FGESEE   LEVVTLACGLIAGDTL  S A TT +TFSQF D++  F  LRF+EE+DGK
Sbjct: 186 KFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEELDGK 245

Query: 243 VPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVP 302
           VPLVHI DV +AHIFCMEQ+SIHGRFLCA+S+LSS EIA+YY LHH  LQQKHG  D+V 
Sbjct: 246 VPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKLDEVA 305

Query: 303 VRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
            RNIK NS+KLI++GF Y YDG+MILED F C
Sbjct: 306 KRNIKMNSKKLIERGFIYKYDGDMILEDVFHC 336

BLAST of Sgr025416 vs. NCBI nr
Match: TYK17664.1 (vestitone reductase-like [Cucumis melo var. makuwa])

HSP 1 Score: 479.2 bits (1232), Expect = 3.1e-131
Identity = 238/332 (71.69%), Postives = 269/332 (81.02%), Query Frame = 0

Query: 3   ASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLL 62
           +SGC   VCVT GSGYVAS  +KKLL  GH VHATLRNL+DESK+G+LK  PNA+T L+L
Sbjct: 6   SSGC-LRVCVTCGSGYVASSFIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNLVL 65

Query: 63  FKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTV 122
           FKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAKMIAK CVE GTV
Sbjct: 66  FKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVELGTV 125

Query: 123 RRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLL 182
           RRLIYTASV+S SPMKDDGSGFKD +DE CWTPL LSYP+ N  + GY+DSKT TEK LL
Sbjct: 126 RRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATEKELL 185

Query: 183 GFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGK 242
            FGESEE   LEVVTLACGLIAGDTL  S A TT +TFSQF D++  F  LRF+EE+DGK
Sbjct: 186 KFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEELDGK 245

Query: 243 VPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVP 302
           VPLVHI DV +AHIFCMEQ+SIHGRFLCA+S+LSS EIA+YY LHH  LQQKHG  D+V 
Sbjct: 246 VPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKLDEVA 305

Query: 303 VRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
            RNIK NS+KLI++GF Y YDG+MILED F C
Sbjct: 306 KRNIKMNSKKLIERGFIYKYDGDMILEDVFHC 336

BLAST of Sgr025416 vs. NCBI nr
Match: XP_004134310.3 (putative anthocyanidin reductase isoform X1 [Cucumis sativus] >KAE8650235.1 hypothetical protein Csa_010630 [Cucumis sativus])

HSP 1 Score: 474.2 bits (1219), Expect = 1.0e-129
Identity = 234/334 (70.06%), Postives = 270/334 (80.84%), Query Frame = 0

Query: 1   MEASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKL 60
           M  S     VCVTGGSGYVAS L+KKLL  GH VHATLRNL DESKVG+LK  PNA+  L
Sbjct: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61  LLFKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESG 120
           +LFKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAK IAK CVE G
Sbjct: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120

Query: 121 TVRRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKW 180
           TVRRLIYTAS++S SPMK+DGSGFKDF+DE+CWTPLNLSYP+ N  L GY+DSKT TEK 
Sbjct: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180

Query: 181 LLGFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVD 240
           LL FG+SEE  GLEVV+L CGL+AG++   S A TT++TFSQF  ++  F  LRF+EE+D
Sbjct: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240

Query: 241 GKVPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDD 300
           GKVPLVHI DV +AHIFCMEQ+SIHGRFLCASS+LSS+EIA+YY LHH  LQQKHG  D+
Sbjct: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 300

Query: 301 VPVRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
           V  RNIK NS+KLI++GF Y YDG+MILEDAF C
Sbjct: 301 VAKRNIKMNSKKLIERGFVYKYDGDMILEDAFHC 334

BLAST of Sgr025416 vs. ExPASy Swiss-Prot
Match: Q5XLY0 (Putative anthocyanidin reductase OS=Ginkgo biloba OX=3311 PE=2 SV=1)

HSP 1 Score: 178.3 bits (451), Expect = 1.5e-43
Identity = 104/330 (31.52%), Postives = 174/330 (52.73%), Query Frame = 0

Query: 4   SGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLF 63
           +G ++TVCVTG +G++ASWLVK+LL+KG+ VHAT+R+  +++KV  L + P A+ +L LF
Sbjct: 9   AGQTTTVCVTGAAGFMASWLVKRLLEKGYIVHATVRDPENKAKVSHLLNLPGATDRLKLF 68

Query: 64  KADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVR 123
           +A++ E   F+  + GC+ VFHVATP          +  + ++     + K C +  +++
Sbjct: 69  RAELCEDGSFDAAVAGCNGVFHVATPTEFMPKDPENDLIKPAIEGTLNVLKSCTKVDSIK 128

Query: 124 RLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLG 183
           R++ T+S  + S   ++ S    ++DE+CWT +N            Y  SKT  E+  L 
Sbjct: 129 RVVVTSSAATVS--INNSSEQNQYIDESCWTDVNFLTSQKPPGW-AYPVSKTLAEQAALK 188

Query: 184 FGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKV 243
           + E      L+VVT+   L+ G  +  +   +  +  S  T       AL+ ++ V G +
Sbjct: 189 YAEEH---SLDVVTVIPVLVVGPAVTPTVPSSVELALSLITGDEFKMGALKGMQFVSGSI 248

Query: 244 PLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPV 303
            LVHI DV  A IF ME+ S  GR++C        ++A +    +P+ +      D    
Sbjct: 249 SLVHIDDVCSAQIFLMEKPSAQGRYICFPVNTGIPQLAEFLSKRYPQYKVPTKFDDVPAT 308

Query: 304 RNIKWNSRKLIDKGFRYNYDGEMILEDAFE 334
             +  +S+KL+D GF + Y  E I + A E
Sbjct: 309 PKLTISSQKLLDCGFSFKYGIEDIYDQAIE 332

BLAST of Sgr025416 vs. ExPASy Swiss-Prot
Match: Q7PCC4 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 3.7e-42
Identity = 103/330 (31.21%), Postives = 170/330 (51.52%), Query Frame = 0

Query: 9   TVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKADIY 68
           T CV GG+G+VAS LVK LLQKG+ V+ T+R+ +++ KV  L         L +F+AD+ 
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELG-DLKIFRADLT 70

Query: 69  EPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLIYT 128
           +   FE PI GCDFVFHVATP+  +      +  + ++     + K C  + +V+R+I T
Sbjct: 71  DELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILT 130

Query: 129 ASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLGFGESE 188
           +S  + +  + DG+G    +DE  WT +            GY  SKT  EK    F E  
Sbjct: 131 SSAAAVTINQLDGTGL--VVDEKNWTDIEF-LTSAKPPTWGYPASKTLAEKAAWKFAEE- 190

Query: 189 EGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLVHI 248
               ++++T+   L+AG +L S    +  +  S  T      + ++ ++ + G V + H+
Sbjct: 191 --NNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHV 250

Query: 249 HDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPVRNIKW 308
            DV  AHIF  E+ S  GR++C ++  S  E+A +    +P+ +      D  P   +  
Sbjct: 251 EDVCQAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSKLII 310

Query: 309 NSRKLIDKGFRYNYDGEMILEDAFECYDKK 339
           +S KL+ +GF + Y  E I +++ E +  K
Sbjct: 311 SSEKLVKEGFSFKYGIEEIYDESVEYFKAK 333

BLAST of Sgr025416 vs. ExPASy Swiss-Prot
Match: Q5FB34 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=1 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 6.2e-42
Identity = 103/330 (31.21%), Postives = 170/330 (51.52%), Query Frame = 0

Query: 9   TVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKADIY 68
           T CV GG+G+VAS LVK LLQKG+ V+ T+R+ +++ KV  L         L +F+AD+ 
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELG-DLKIFRADLT 70

Query: 69  EPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLIYT 128
           +   FE PI GCDFVFHVATP+  +      +  + ++     + K C  + +V+R+I T
Sbjct: 71  DELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAIQGVVNVMKACTRAKSVKRVILT 130

Query: 129 ASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLGFGESE 188
           +S  + +  + DG+G    +DE  WT +            GY  SKT  EK    F E  
Sbjct: 131 SSAAAVTINQLDGTGL--VVDEKNWTDIEF-LTSAKPPTWGYPASKTLAEKAAWKFAEE- 190

Query: 189 EGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLVHI 248
               ++++T+   L+AG +L S    +  +  S  T      + ++ ++ + G V + H+
Sbjct: 191 --NNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHV 250

Query: 249 HDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPVRNIKW 308
            DV  AHIF  E+ S  GR++C ++  S  E+A +    +P+ +      D  P   +  
Sbjct: 251 EDVCRAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPPKSKLII 310

Query: 309 NSRKLIDKGFRYNYDGEMILEDAFECYDKK 339
           +S KL+ +GF + Y  E I +++ E +  K
Sbjct: 311 SSEKLVKEGFSFKYGIEEIYDESVEYFKAK 333

BLAST of Sgr025416 vs. ExPASy Swiss-Prot
Match: D7U6G6 (Anthocyanidin reductase ((2S)-flavan-3-ol-forming) OS=Vitis vinifera OX=29760 GN=ANR PE=3 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 6.9e-41
Identity = 103/330 (31.21%), Postives = 169/330 (51.21%), Query Frame = 0

Query: 9   TVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKADIY 68
           T CV GG+G+VAS LVK LLQKG+ V+ T+R+ +++ KV  L         L +F+AD+ 
Sbjct: 11  TACVVGGTGFVASLLVKLLLQKGYAVNTTVRDPDNQKKVSHLLELQELG-DLKIFRADLT 70

Query: 69  EPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLIYT 128
           +   FE PI GCDFVFHVATP+  +      +  + +V     + K C  + +V+R+I T
Sbjct: 71  DELSFEAPIAGCDFVFHVATPVHFASEDPENDMIKPAVQGVVNVMKACTRAKSVKRVILT 130

Query: 129 ASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLGFGESE 188
           +S  + +  + DG+G    +DE  WT +            GY  SKT  EK    F E  
Sbjct: 131 SSAAAVTINQLDGTGL--VVDEKNWTDIEF-LTSAKPPTWGYPASKTLAEKAAWKFAEE- 190

Query: 189 EGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLVHI 248
               ++++T+   L+AG +L S    +  +  S  T      + ++ ++ + G V + H+
Sbjct: 191 --NNIDLITVIPTLMAGSSLTSDVPSSIGLAMSLITGNEFLINGMKGMQMLSGSVSIAHV 250

Query: 249 HDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPVRNIKW 308
            DV  AHIF  E+ S  GR++C ++  S  E+A +    +P+ +      D      +  
Sbjct: 251 EDVCRAHIFVAEKESASGRYICCAANTSVPELAKFLSKRYPQYKVPTDFGDFPSKSKLII 310

Query: 309 NSRKLIDKGFRYNYDGEMILEDAFECYDKK 339
           +S KL+ +GF + Y  E I +++ E +  K
Sbjct: 311 SSDKLVKEGFSFKYGIEEIYDESVEYFKAK 333

BLAST of Sgr025416 vs. ExPASy Swiss-Prot
Match: Q9SEV0 (Anthocyanidin reductase OS=Arabidopsis thaliana OX=3702 GN=BAN PE=1 SV=2)

HSP 1 Score: 167.5 bits (423), Expect = 2.6e-40
Identity = 98/332 (29.52%), Postives = 171/332 (51.51%), Query Frame = 0

Query: 7   SSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKAD 66
           S   CV GG+G +AS L+K LLQ G+ V+ T+R+  +E K+  L+        L +FKAD
Sbjct: 10  SKKACVIGGTGNLASILIKHLLQSGYKVNTTVRDPENEKKIAHLRKLQELG-DLKIFKAD 69

Query: 67  IYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLI 126
           + + + FE    GC+++FHVATP+        K+  + ++     + K C++S +V+R+I
Sbjct: 70  LTDEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVI 129

Query: 127 YTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLGFGE 186
           YT+S  + S     G+G    M+E  WT +         N  GY  SK   EK    F +
Sbjct: 130 YTSSAAAVSINNLSGTGI--VMNEENWTDVEFLTEEKPFNW-GYPISKVLAEKTAWEFAK 189

Query: 187 SEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLV 246
             +   + +VT+   LIAG++L S    +  ++ S  T K      L+ ++++ G +  V
Sbjct: 190 ENK---INLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFV 249

Query: 247 HIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPVRNI 306
           H+ D+  AH+F  E+ +  GR++C +   S  EIA +    +P+        + + +  +
Sbjct: 250 HVDDLARAHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSIPKL 309

Query: 307 KWNSRKLIDKGFRYNYDGEMILEDAFECYDKK 339
             +S+KLI++GFR+ Y    + +   E ++ K
Sbjct: 310 TLSSQKLINEGFRFEYGINEMYDQMIEYFESK 334

BLAST of Sgr025416 vs. ExPASy TrEMBL
Match: A0A6J1D0X7 (anthocyanidin reductase ((2S)-flavan-3-ol-forming)-like OS=Momordica charantia OX=3673 GN=LOC111016282 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 6.3e-154
Identity = 271/344 (78.78%), Postives = 299/344 (86.92%), Query Frame = 0

Query: 4   SGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLF 63
           SGC S VCVTGGSGYVAS LVKKLLQKGHTVHATLRNLND+SKV LL + PNA+ +L+LF
Sbjct: 6   SGCGSRVCVTGGSGYVASLLVKKLLQKGHTVHATLRNLNDQSKVRLLNTLPNAAHRLVLF 65

Query: 64  KADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVR 123
           +ADIY+P GF PPI+GCDFVFHVATPL H+QGSQYKNRT+ASV AA+MIAKFCVESGTVR
Sbjct: 66  QADIYQPEGFRPPIQGCDFVFHVATPLHHTQGSQYKNRTDASVGAARMIAKFCVESGTVR 125

Query: 124 RLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLG 183
           RLIYTASV+SASPMKDDG+GFKDFMDETCWTPLNLSYP+ N   EGY+DSK+ TE+ LL 
Sbjct: 126 RLIYTASVVSASPMKDDGTGFKDFMDETCWTPLNLSYPFSNPTFEGYVDSKSATERELLE 185

Query: 184 FGE---SEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQF---TDKAPAFHALRFVE 243
           FG     E GGGLEVVTLACGLI GDTL    AETTIITFSQF    D  P +HALRF+E
Sbjct: 186 FGARQVQEGGGGLEVVTLACGLIVGDTLHCCAAETTIITFSQFIHQDDAQPFYHALRFLE 245

Query: 244 EVDGKVPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGV 303
           E+DGKVPLVHIHDV DAHIFCMEQSS+HGRFLCASS+LSSS+IA+YYRLHHP+LQQK+G 
Sbjct: 246 ELDGKVPLVHIHDVCDAHIFCMEQSSLHGRFLCASSFLSSSDIATYYRLHHPQLQQKYGN 305

Query: 304 SDDVPVRNIKWNSRKLIDKGFRYNYDGEMILEDAFECYDKKEIS 342
           SDDVP+RNIKWNSRKLI+KGF Y YDGEMILEDA EC   K IS
Sbjct: 306 SDDVPIRNIKWNSRKLIEKGFVYKYDGEMILEDALECCKNKAIS 349

BLAST of Sgr025416 vs. ExPASy TrEMBL
Match: A0A5A7U5J5 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G001230 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.7e-133
Identity = 240/332 (72.29%), Postives = 271/332 (81.63%), Query Frame = 0

Query: 3   ASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLL 62
           +SGC   VCVTGGSGYVAS L+KKLL  GH VHATLRNL+DESK+G+LK  PNA+T L+L
Sbjct: 6   SSGC-LRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNLVL 65

Query: 63  FKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTV 122
           FKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAKMIAK CVE GTV
Sbjct: 66  FKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVELGTV 125

Query: 123 RRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLL 182
           RRLIYTASV+S SPMKDDGSGFKD +DE CWTPL LSYP+ N  + GY+DSKT TEK LL
Sbjct: 126 RRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATEKELL 185

Query: 183 GFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGK 242
            FGESEE   LEVVTLACGLIAGDTL  S A TT +TFSQF D++  F  LRF+EE+DGK
Sbjct: 186 KFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEELDGK 245

Query: 243 VPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVP 302
           VPLVHI DV +AHIFCMEQ+SIHGRFLCA+S+LSS EIA+YY LHH  LQQKHG  D+V 
Sbjct: 246 VPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKLDEVA 305

Query: 303 VRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
            RNIK NS+KLI++GF Y YDG+MILED F C
Sbjct: 306 KRNIKMNSKKLIERGFIYKYDGDMILEDVFHC 336

BLAST of Sgr025416 vs. ExPASy TrEMBL
Match: A0A1S3AUS8 (vestitone reductase-like OS=Cucumis melo OX=3656 GN=LOC103483203 PE=3 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 4.7e-133
Identity = 240/332 (72.29%), Postives = 271/332 (81.63%), Query Frame = 0

Query: 3   ASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLL 62
           +SGC   VCVTGGSGYVAS L+KKLL  GH VHATLRNL+DESK+G+LK  PNA+T L+L
Sbjct: 6   SSGC-LRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNLVL 65

Query: 63  FKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTV 122
           FKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAKMIAK CVE GTV
Sbjct: 66  FKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVELGTV 125

Query: 123 RRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLL 182
           RRLIYTASV+S SPMKDDGSGFKD +DE CWTPL LSYP+ N  + GY+DSKT TEK LL
Sbjct: 126 RRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATEKELL 185

Query: 183 GFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGK 242
            FGESEE   LEVVTLACGLIAGDTL  S A TT +TFSQF D++  F  LRF+EE+DGK
Sbjct: 186 KFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEELDGK 245

Query: 243 VPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVP 302
           VPLVHI DV +AHIFCMEQ+SIHGRFLCA+S+LSS EIA+YY LHH  LQQKHG  D+V 
Sbjct: 246 VPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKLDEVA 305

Query: 303 VRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
            RNIK NS+KLI++GF Y YDG+MILED F C
Sbjct: 306 KRNIKMNSKKLIERGFIYKYDGDMILEDVFHC 336

BLAST of Sgr025416 vs. ExPASy TrEMBL
Match: A0A5D3D2U9 (Vestitone reductase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G005360 PE=3 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 1.5e-131
Identity = 238/332 (71.69%), Postives = 269/332 (81.02%), Query Frame = 0

Query: 3   ASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLL 62
           +SGC   VCVT GSGYVAS  +KKLL  GH VHATLRNL+DESK+G+LK  PNA+T L+L
Sbjct: 6   SSGC-LRVCVTCGSGYVASSFIKKLLLNGHIVHATLRNLDDESKIGILKRLPNATTNLVL 65

Query: 63  FKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTV 122
           FKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAKMIAK CVE GTV
Sbjct: 66  FKADIYEPHEFEAAIRGCHIVFHVATPLHHTHATQYSDVTEASVAAAKMIAKLCVELGTV 125

Query: 123 RRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLL 182
           RRLIYTASV+S SPMKDDGSGFKD +DE CWTPL LSYP+ N  + GY+DSKT TEK LL
Sbjct: 126 RRLIYTASVVSMSPMKDDGSGFKDVLDENCWTPLKLSYPFSNSTVVGYVDSKTATEKELL 185

Query: 183 GFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGK 242
            FGESEE   LEVVTLACGLIAGDTL  S A TT +TFSQF D++  F  LRF+EE+DGK
Sbjct: 186 KFGESEESMKLEVVTLACGLIAGDTLHPSPAATTTVTFSQFIDESEPFKLLRFLEELDGK 245

Query: 243 VPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVP 302
           VPLVHI DV +AHIFCMEQ+SIHGRFLCA+S+LSS EIA+YY LHH  LQQKHG  D+V 
Sbjct: 246 VPLVHIDDVCEAHIFCMEQTSIHGRFLCATSFLSSPEIANYYHLHHSHLQQKHGKLDEVA 305

Query: 303 VRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
            RNIK NS+KLI++GF Y YDG+MILED F C
Sbjct: 306 KRNIKMNSKKLIERGFIYKYDGDMILEDVFHC 336

BLAST of Sgr025416 vs. ExPASy TrEMBL
Match: A0A0A0L371 (Epimerase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G119707 PE=3 SV=1)

HSP 1 Score: 474.2 bits (1219), Expect = 4.8e-130
Identity = 234/334 (70.06%), Postives = 270/334 (80.84%), Query Frame = 0

Query: 1   MEASGCSSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKL 60
           M  S     VCVTGGSGYVAS L+KKLL  GH VHATLRNL DESKVG+LK  PNA+  L
Sbjct: 1   MAVSSDCLRVCVTGGSGYVASSLIKKLLLNGHIVHATLRNLGDESKVGILKRLPNATNNL 60

Query: 61  LLFKADIYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESG 120
           +LFKADIYEP+ FE  IRGC  VFHVATPL H+  +QY + TEASVAAAK IAK CVE G
Sbjct: 61  VLFKADIYEPHQFEAAIRGCHIVFHVATPLHHTHATQYMDVTEASVAAAKKIAKLCVELG 120

Query: 121 TVRRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKW 180
           TVRRLIYTAS++S SPMK+DGSGFKDF+DE+CWTPLNLSYP+ N  L GY+DSKT TEK 
Sbjct: 121 TVRRLIYTASIVSMSPMKEDGSGFKDFLDESCWTPLNLSYPFSNSFLVGYLDSKTITEKE 180

Query: 181 LLGFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVD 240
           LL FG+SEE  GLEVV+L CGL+AG++   S A TT++TFSQF  ++  F  LRF+EE+D
Sbjct: 181 LLKFGKSEESKGLEVVSLVCGLVAGESPHPSAAITTMVTFSQFIHESEPFKFLRFLEELD 240

Query: 241 GKVPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDD 300
           GKVPLVHI DV +AHIFCMEQ+SIHGRFLCASS+LSS+EIA+YY LHH  LQQKHG  D+
Sbjct: 241 GKVPLVHIDDVCEAHIFCMEQTSIHGRFLCASSFLSSTEIANYYHLHHSHLQQKHGKLDE 300

Query: 301 VPVRNIKWNSRKLIDKGFRYNYDGEMILEDAFEC 335
           V  RNIK NS+KLI++GF Y YDG+MILEDAF C
Sbjct: 301 VAKRNIKMNSKKLIERGFVYKYDGDMILEDAFHC 334

BLAST of Sgr025416 vs. TAIR 10
Match: AT1G61720.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.9e-41
Identity = 98/332 (29.52%), Postives = 171/332 (51.51%), Query Frame = 0

Query: 7   SSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKAD 66
           S   CV GG+G +AS L+K LLQ G+ V+ T+R+  +E K+  L+        L +FKAD
Sbjct: 10  SKKACVIGGTGNLASILIKHLLQSGYKVNTTVRDPENEKKIAHLRKLQELG-DLKIFKAD 69

Query: 67  IYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLI 126
           + + + FE    GC+++FHVATP+        K+  + ++     + K C++S +V+R+I
Sbjct: 70  LTDEDSFESSFSGCEYIFHVATPINFKSEDPEKDMIKPAIQGVINVLKSCLKSKSVKRVI 129

Query: 127 YTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEGYIDSKTKTEKWLLGFGE 186
           YT+S  + S     G+G    M+E  WT +         N  GY  SK   EK    F +
Sbjct: 130 YTSSAAAVSINNLSGTGI--VMNEENWTDVEFLTEEKPFNW-GYPISKVLAEKTAWEFAK 189

Query: 187 SEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLV 246
             +   + +VT+   LIAG++L S    +  ++ S  T K      L+ ++++ G +  V
Sbjct: 190 ENK---INLVTVIPALIAGNSLLSDPPSSLSLSMSFITGKEMHVTGLKEMQKLSGSISFV 249

Query: 247 HIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVPVRNI 306
           H+ D+  AH+F  E+ +  GR++C +   S  EIA +    +P+        + + +  +
Sbjct: 250 HVDDLARAHLFLAEKETASGRYICCAYNTSVPEIADFLIQRYPKYNVLSEFEEGLSIPKL 309

Query: 307 KWNSRKLIDKGFRYNYDGEMILEDAFECYDKK 339
             +S+KLI++GFR+ Y    + +   E ++ K
Sbjct: 310 TLSSQKLINEGFRFEYGINEMYDQMIEYFESK 334

BLAST of Sgr025416 vs. TAIR 10
Match: AT5G42800.1 (dihydroflavonol 4-reductase )

HSP 1 Score: 167.2 bits (422), Expect = 2.4e-41
Identity = 109/335 (32.54%), Postives = 173/335 (51.64%), Query Frame = 0

Query: 9   TVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKADIY 68
           TVCVTG SG++ SWLV +LL++G+ V AT+R+  +  KV  L   PNA T L L+KAD+ 
Sbjct: 7   TVCVTGASGFIGSWLVMRLLERGYFVRATVRDPGNLKKVQHLLDLPNAKTLLTLWKADLS 66

Query: 69  EPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLIYT 128
           E   ++  I GCD VFHVATP+            + +V     I K CV++ TVRR ++T
Sbjct: 67  EEGSYDDAINGCDGVFHVATPMDFESKDPENEVIKPTVNGMLGIMKACVKAKTVRRFVFT 126

Query: 129 ASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEG--YIDSKTKTEKWLLGFGE 188
           +S  + +  +      K+  DE  W+ L      ++  + G  Y  SKT  EK    F E
Sbjct: 127 SSAGTVNVEEHQ----KNVYDENDWSDLEF---IMSKKMTGWMYFVSKTLAEKAAWDFAE 186

Query: 189 SEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFVEEVDGKVPLV 248
            +   GL+ +++   L+ G  + +S   + I   S  T     +  +R       +   V
Sbjct: 187 EK---GLDFISIIPTLVVGPFITTSMPPSLITALSPITRNEAHYSIIR-------QGQYV 246

Query: 249 HIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQK---HGVSDDVPV 308
           H+ D+ +AHIF  EQ++  GR++C+S   +   I+ + R  +PE        GV ++  +
Sbjct: 247 HLDDLCNAHIFLYEQAAAKGRYICSSHDATILTISKFLRPKYPEYNVPSTFEGVDEN--L 306

Query: 309 RNIKWNSRKLIDKGFRYNYD-GEMILEDAFECYDK 338
           ++I+++S+KL D GF + Y   EM +E    C  K
Sbjct: 307 KSIEFSSKKLTDMGFNFKYSLEEMFIESIETCRQK 322

BLAST of Sgr025416 vs. TAIR 10
Match: AT2G45400.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 160.6 bits (405), Expect = 2.3e-39
Identity = 106/330 (32.12%), Postives = 165/330 (50.00%), Query Frame = 0

Query: 10  VCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESK--VGLLKSFPNASTKLLLFKADI 69
           VCVTGGSG+VASWL+ +LLQ+G++V AT+R  ++ +K  +  L   P AS +L +F AD+
Sbjct: 40  VCVTGGSGFVASWLIMRLLQRGYSVRATVRTNSEGNKKDISYLTELPFASERLQIFTADL 99

Query: 70  YEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNRTEASVAAAKMIAKFCVESGTVRRLIY 129
            EP  F+P I GC  VFHVA P+  +     +  T+ +V     I K C+++ TV+R  Y
Sbjct: 100 NEPESFKPAIEGCKAVFHVAHPMDPNSNETEETVTKRTVQGLMGILKSCLDAKTVKRFFY 159

Query: 130 TASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNL-EGYIDSKTKTEKWLLGFGE 189
           T+S ++      +G G  + +DE+ W+ + +        +   Y+ SK   E   L FG 
Sbjct: 160 TSSAVTVFYSGGNGGGGGE-VDESVWSDVEVFRNQKEKRVSSSYVVSKMAAETAALEFGG 219

Query: 190 SEEGGGLEVVTLACGLIAGDTLQSSTAETTIIT----FSQFTDKAPAFHALRFVEEVDGK 249
                GLEVVTL   L+ G  + SS   +  I+    F  + +K            +   
Sbjct: 220 K---NGLEVVTLVIPLVVGPFISSSLPSSVFISLAMLFGNYKEK-----------YLFDT 279

Query: 250 VPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHHPELQQKHGVSDDVP 309
             +VHI DV  A IF +E+    GR++C+S  +   E+  +     P+ Q      +   
Sbjct: 280 YNMVHIDDVARAMIFLLEKPVAKGRYICSSVEMKIDEVFEFLSTKFPQFQLPSIDLNKYK 339

Query: 310 V-RNIKWNSRKLIDKGFRYNYDGEMILEDA 332
           V + +  +S+KL   GF + Y  E I   A
Sbjct: 340 VEKRMGLSSKKLKSAGFEFKYGAEEIFSGA 354

BLAST of Sgr025416 vs. TAIR 10
Match: AT4G27250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 156.8 bits (395), Expect = 3.3e-38
Identity = 104/343 (30.32%), Postives = 173/343 (50.44%), Query Frame = 0

Query: 7   SSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKAD 66
           ++T CVTG SGY+ SWLVK LLQ+G+TVHATLR+L   +K    +S    + +L LF+AD
Sbjct: 10  TATYCVTGASGYIGSWLVKSLLQRGYTVHATLRDL---AKSEYFQSKWKENERLRLFRAD 69

Query: 67  IYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNR--------TEASVAAAKMIAKFCVE 126
           + +   F+  ++GCD VFHVA  +     S + N          E ++   + +   C++
Sbjct: 70  LRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVLSSCLK 129

Query: 127 SGTVRRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEG--YIDSKTK 186
           S +V+R+++T+S+ S    KD+    + F+DETC   ++     L     G  Y+ SK  
Sbjct: 130 SKSVKRVVFTSSI-STLTAKDENERMRSFVDETCKAHVD---HVLKTQASGWIYVLSKLV 189

Query: 187 TEKWLLGFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFV 246
           +E+    + +     G+++V++    ++G  L      +  +  S  T  +  F  L  V
Sbjct: 190 SEEEAFRYAKER---GMDLVSVITTTVSGPFLTPFVPSSVQVLLSPITGDSKLFAILSAV 249

Query: 247 EEVDGKVPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHH---PELQQ 306
            +  G + LVHI D+  AH+F MEQ    G+++C    +   E+     LHH     L +
Sbjct: 250 NKRMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHEL----MLHHFSKDYLCK 309

Query: 307 KHGVSDDVPVRNIK---WNSRKLIDKGFRYNYDGEMILEDAFE 334
              V++D   R       +S+KL + GF Y Y  E I++   +
Sbjct: 310 VQKVNEDEEERECMKPIISSKKLRELGFEYKYGIEEIVDQTID 338

BLAST of Sgr025416 vs. TAIR 10
Match: AT4G27250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 147.1 bits (370), Expect = 2.6e-35
Identity = 102/343 (29.74%), Postives = 170/343 (49.56%), Query Frame = 0

Query: 7   SSTVCVTGGSGYVASWLVKKLLQKGHTVHATLRNLNDESKVGLLKSFPNASTKLLLFKAD 66
           ++T CVTG SGY+ SWLVK LLQ+G+TVHATLR+L   +K    +S    + +L LF+AD
Sbjct: 10  TATYCVTGASGYIGSWLVKSLLQRGYTVHATLRDL---AKSEYFQSKWKENERLRLFRAD 69

Query: 67  IYEPNGFEPPIRGCDFVFHVATPLLHSQGSQYKNR--------TEASVAAAKMIAKFCVE 126
           + +   F+  ++GCD VFHVA  +     S + N          E ++   + +   C++
Sbjct: 70  LRDDGSFDDAVKGCDGVFHVAASMEFDISSDHVNLESYVQSKVIEPALKGVRNVLSSCLK 129

Query: 127 SGTVRRLIYTASVLSASPMKDDGSGFKDFMDETCWTPLNLSYPYLNHNLEG--YIDSKTK 186
           S +V+R+++T+S+ S    KD+    + F+DETC   ++     L     G  Y+ SK  
Sbjct: 130 SKSVKRVVFTSSI-STLTAKDENERMRSFVDETCKAHVD---HVLKTQASGWIYVLSKLV 189

Query: 187 TEKWLLGFGESEEGGGLEVVTLACGLIAGDTLQSSTAETTIITFSQFTDKAPAFHALRFV 246
           +E+    + +     G+++V++    ++G  L      +  +  S  T           V
Sbjct: 190 SEEEAFRYAKER---GMDLVSVITTTVSGPFLTPFVPSSVQVLLSPIT----------AV 249

Query: 247 EEVDGKVPLVHIHDVRDAHIFCMEQSSIHGRFLCASSYLSSSEIASYYRLHH---PELQQ 306
            +  G + LVHI D+  AH+F MEQ    G+++C    +   E+     LHH     L +
Sbjct: 250 NKRMGSIALVHIEDICRAHLFLMEQPKAKGQYICCVDNIDMHEL----MLHHFSKDYLCK 309

Query: 307 KHGVSDDVPVRNIK---WNSRKLIDKGFRYNYDGEMILEDAFE 334
              V++D   R       +S+KL + GF Y Y  E I++   +
Sbjct: 310 VQKVNEDEEERECMKPIISSKKLRELGFEYKYGIEEIVDQTID 328