Sgr025362 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025362 vs. NCBI nr
Match: KVH92884.1 (Aminotransferase, class I/classII [Cynara cardunculus var. scolymus])

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 648/1237 (52.38%), Postives = 802/1237 (64.83%), Query Frame = 0

Query: 7    GISDSGEQNSIPKKSKKKLLLALSATLLLVGAIVG-IVAGVTKSRNSDR------SEAHA 66
            G  D  E+ +  +K++K++L+ + + LLL+  I+G + A +   RN+ +      S +  
Sbjct: 11   GKLDPAEEQAFRRKTRKRILILILSLLLLLALIIGAVTATLLHKRNNHKDASPSSSSSAQ 70

Query: 67   ILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEA--SYFRVQKLA 126
             +K+ CS TLYPD C+S+IS  D      ++ + +++LS+ +   ++ A  S F    + 
Sbjct: 71   SIKAVCSQTLYPDSCYSSISQLD--QSNSTDPEHLLKLSLQVVFNSLSALSSSFPHSFIK 130

Query: 127  ALKNITHRERLALHDCLETIDETLDELHKAIA--DLHEYPNKKSLTQHADDLKTLLSSAI 186
            +  N T ++  AL  C   +D+ LD L  +I+  DL    +K       +DLKT LS+A+
Sbjct: 131  STTNDTLKQ--ALQVCATLLDDALDYLRDSISTMDLKSADDKLLTLTKIEDLKTWLSTAL 190

Query: 187  TNQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMGSRK 246
            TNQ+TCLD     +   +L    +   +   H  SN+LA+V  +  + I    +    RK
Sbjct: 191  TNQDTCLDALQEIDPSSTLLADTKSLMQNSTHYASNSLAIVSKI--SGILRKLNLPMHRK 250

Query: 247  LMEEADNDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKK 306
            L+ E        +PEW+S G RRLLQ    TP+V VAADG+G+  T+  A+A  P KSK 
Sbjct: 251  LLGE--------FPEWVSPGVRRLLQVGRPTPNVTVAADGTGDVTTIKEAMARVPKKSKT 310

Query: 307  RYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEG 366
             +II IK GVY ENV + K   N+M  GDG+  +I++   N +DG  TF++AT A  G G
Sbjct: 311  MFIIHIKEGVYLENVVLDKSFWNVMIYGDGKDKSIVSARLNFIDGIPTFSTATFAVAGRG 370

Query: 367  FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGT 426
            F+A D+ F+NTAG  KHQAVA R G+D S  Y+C   A+QDTLY HSNRQFY +C ++GT
Sbjct: 371  FVAIDMGFKNTAGAEKHQAVAFRSGSDFSVLYRCSFDAFQDTLYPHSNRQFYRDCDVTGT 430

Query: 427  VDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPV 486
            +DFIFGNAA +FQNC I  R+P   Q   +TAQG+ DPNQNTGI IQK  I    +L   
Sbjct: 431  IDFIFGNAAVVFQNCKIMPRQPLPRQFVTITAQGKKDPNQNTGISIQKCDISPFDNL--- 490

Query: 487  QKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEW-SGNFALNTLFYGEYQNTGPGAS 546
                PT+LGRPWK++S TVIMQS+I   + P GW  W  G     ++ YGEY NTG GA+
Sbjct: 491  --TAPTYLGRPWKDFSTTVIMQSNIGSFLHPSGWISWVQGVDPPKSILYGEYLNTGAGAA 550

Query: 547  TSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLRGKKQEKKKNFAPPSPEIENRHNKNR 606
               RVTW G++ I + +EA  FT   FI          +++ ++F               
Sbjct: 551  VDKRVTWDGYKAILTDSEAGRFTVDSFI----------EDRIRSF--------------- 610

Query: 607  HSKSARCSGVEQTGMGINGGRGKLKDAMQWNCMSHFNVRLRDLVSVDSDKGRCVQLVLSL 666
                                                                CV  +  +
Sbjct: 611  ----------------------------------------------------CVAFLPPI 670

Query: 667  SFLVIRFFDLSYGASFRRLPSGFHSDHQPQVIVVDILVIVPIPYSFRVFAFCAGVWITSF 726
            S   +    L    + R+  +     H+ + I+      +  P+     +       +S 
Sbjct: 671  SPTDV----LKTNTAMRKFVA-----HRAKTIITKATASLFTPHRIHPTS-------SSS 730

Query: 727  GCSLPSVYMYSSPESGR-----ESGCSIRWKMTTPGYFETRFLHFMVLKCEYAVRGEIVA 786
              S    Y Y +    R     +S  S+    + P       L+  VLKCEYAVRGEIV+
Sbjct: 731  SSSSDFYYYYYTRTLNRFFTSLDSSTSMSSDKSAPP-LTVESLNPKVLKCEYAVRGEIVS 790

Query: 787  LAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSILNRNEIQG 846
            LAQKLQ++L   PGS  F+EILYCNIGNPQSLGQQPITFFREVLALCDHP+IL+++E QG
Sbjct: 791  LAQKLQQDLQANPGSHPFEEILYCNIGNPQSLGQQPITFFREVLALCDHPTILDKSETQG 850

Query: 847  LFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFPADPADLFLTDG 906
            LFS D+I +A QIL QIPGRATGAYSHSQGIKGLRDAIAAGIEARDGF ADP D+FLTDG
Sbjct: 851  LFSADSIERAWQILDQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFSADPNDIFLTDG 910

Query: 907  ASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTL--VPYYLDEASGWGLEISE 966
            ASPAVHMMMQLLI SEKDGI CPIPQYPLYSASIALHGGTL  VPYYLDEA+GWGLE+SE
Sbjct: 911  ASPAVHMMMQLLIRSEKDGIFCPIPQYPLYSASIALHGGTLVCVPYYLDEATGWGLEVSE 970

Query: 967  LKKELESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGLVLLADEVYQENI 1026
            LKK+LE A+  GI+VRALVVINPGNPTGQVL +ENQ  IVEFCK+EGLVLLADEVYQENI
Sbjct: 971  LKKQLEIARQNGITVRALVVINPGNPTGQVLAEENQRDIVEFCKKEGLVLLADEVYQENI 1030

Query: 1027 YVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYMEVTGFSADVREQI 1086
            Y  +K+FHSFKK+SRSMGYG+ DI LVSFQSVSKGYYGECGKRGGYMEVTGFS +VREQI
Sbjct: 1031 YAPNKQFHSFKKVSRSMGYGDKDIPLVSFQSVSKGYYGECGKRGGYMEVTGFSPEVREQI 1090

Query: 1087 YKVASVNLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLARRAKTLEAAFNSL 1146
            YKVASVNLCSNISGQILASLVM+PPKVGD  Y+SY AERDGIL SLARRAKTLE A NSL
Sbjct: 1091 YKVASVNLCSNISGQILASLVMSPPKVGDESYDSYFAERDGILQSLARRAKTLEDALNSL 1134

Query: 1147 ENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATGIVVVPGSGFGQV 1206
            E V+CNKAEGAMYLFP I+LP KAIK AEAA  APDA+Y   LLNATGIVVVPGSGFGQV
Sbjct: 1151 EGVSCNKAEGAMYLFPRIRLPNKAIKEAEAAKKAPDAYYASRLLNATGIVVVPGSGFGQV 1134

Query: 1207 PGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            PGTWHFRCTILPQE+KIPA+VSRLT+FH+ FM E+RD
Sbjct: 1211 PGTWHFRCTILPQEEKIPAIVSRLTEFHKKFMDEFRD 1134

BLAST of Sgr025362 vs. NCBI nr
Match: PWA78825.1 (pyridoxal phosphate-dependent transferase [Artemisia annua])

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 622/1231 (50.53%), Postives = 769/1231 (62.47%), Query Frame = 0

Query: 7    GISDSGEQNSIPKKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDRSEAHA------- 66
            G  D  E+ +  +K++K+L++ + + +LLV  I+G V GV  SR++    A +       
Sbjct: 11   GKVDPVEEQAFRRKTRKRLVILIVSVVLLVCVIIGAVVGVIHSRSNGNDSADSSGSSSSS 70

Query: 67   --ILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAV-EASYFRVQKL 126
               +K+ CS T YP+ C++++S  D      ++ +++++LS+ +   ++ E S F     
Sbjct: 71   AQSIKAVCSQTRYPESCYTSLS--DINKSNSTDPEELLKLSLEVVYNSLSELSLFPKNME 130

Query: 127  AALKNITHRERLALHDCLETIDETLDELHKAIADLHEYPNKKSLT-QHADDLKTLLSSAI 186
               K++  ++  AL  C   +D+ +D L  +++ +      K L+    DDLKT LS+AI
Sbjct: 131  NLTKDVNVKK--ALQVCGIVLDDAVDYLRDSMSSMDVKSGDKLLSVTKIDDLKTWLSTAI 190

Query: 187  TNQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMGSRK 246
            TNQETCLD  S DE + +    ++   +      SN+LA+V  +  + I    +    RK
Sbjct: 191  TNQETCLD--SLDEMNATFIDDIKLKMQNSSEYASNSLAIVSKI--SGILGKFNLPIHRK 250

Query: 247  LMEEADNDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKK 306
            L+E     +   +P W++ G RRLLQ+   TP+V VA DG+G+  T+  A+A  P KSK 
Sbjct: 251  LLETGSVSE---FPAWVNPGVRRLLQTGRPTPNVTVATDGTGDVTTLKEAMAKVPKKSKT 310

Query: 307  RYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEG 366
            R+II IK G YRENV + K   N+M  GDG++ +++ G  N VDG+ TF++AT A  G G
Sbjct: 311  RFIIYIKTGKYRENVVLDKSFWNVMIYGDGKTKSVVVGRLNFVDGTPTFSTATFAVQGRG 370

Query: 367  FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGT 426
            F+A D+ F N AG  KHQAVA R G+D S FY+C   A+QDTLY HSNRQFY  C ++GT
Sbjct: 371  FVAMDMGFINEAGAEKHQAVAFRSGSDFSVFYRCSFDAFQDTLYPHSNRQFYRECDVTGT 430

Query: 427  VDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPV 486
            +DFIFGNAA +FQ+C I  R+P   Q   +TAQG+ DPNQNTGI IQK       D+ P 
Sbjct: 431  IDFIFGNAAVVFQSCKIMPRQPLKNQFVTITAQGKKDPNQNTGISIQK------CDITPF 490

Query: 487  QK-NFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEW-SGNFALNTLFYGEYQNTGPGA 546
                 PT+LGRPWKE+S TVIMQS+I   ++P GW  W SG     +++YGEY NTG GA
Sbjct: 491  DALTAPTYLGRPWKEFSTTVIMQSTIGSFLNPVGWISWVSGVDPPASIYYGEYLNTGLGA 550

Query: 547  STSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLRGKKQEKKKNFAPPSPEIENRHNKN 606
            S + RV+W G++   ++++A  FT                                    
Sbjct: 551  SVAKRVSWAGYKPSMTSSDAGKFT------------------------------------ 610

Query: 607  RHSKSARCSGVEQTGMGINGGRGKLKDAMQWNCMSHFNVRLRDLVSVDSDKGRCVQLVLS 666
                                                                        
Sbjct: 611  ------------------------------------------------------------ 670

Query: 667  LSFLVIRFFDLSYGASFRRLPSGFHSDHQPQVIVVDILVIVPIPYSFRVFAFCAGVWITS 726
                                                                        
Sbjct: 671  ------------------------------------------------------------ 730

Query: 727  FGCSLPSVYMYSSPESGRESGCSIRWKMTTPGYFETRFLHFMVLKCEYAVRGEIVALAQK 786
                                                      VLKCEYAVRGEIV+LAQ 
Sbjct: 731  ------------------------------------------VLKCEYAVRGEIVSLAQN 790

Query: 787  LQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSILNRNEIQGLFSE 846
            LQ++L   PG+  F+EI+YCNIGNPQSLGQQPITFFREVLALCDHP+IL+++E QGLFS 
Sbjct: 791  LQQDLKANPGTHPFEEIIYCNIGNPQSLGQQPITFFREVLALCDHPTILDKSETQGLFSA 850

Query: 847  DAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFPADPADLFLTDGASPA 906
            D+IN+A +IL QIPGRATGAYSHSQG+KGLRD IAAGIEARDGFPA+P D+FLTDGASPA
Sbjct: 851  DSINRAWEILDQIPGRATGAYSHSQGVKGLRDTIAAGIEARDGFPANPDDIFLTDGASPA 910

Query: 907  VHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYLDEASGWGLEISELKKELE 966
            VHMMMQLLI SE DGI CPIPQYPLYSASIALHGGTLVPYYLDEA+GWGLEISELKK+LE
Sbjct: 911  VHMMMQLLIRSESDGIFCPIPQYPLYSASIALHGGTLVPYYLDEATGWGLEISELKKQLE 970

Query: 967  SAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGLVLLADEVYQENIYVRDKK 1026
            +A+SKGI+VRALVVINPGNPTGQVL +ENQ  IVEFCK+EGLVLLADEVYQENIYV +K+
Sbjct: 971  TARSKGITVRALVVINPGNPTGQVLAEENQRDIVEFCKKEGLVLLADEVYQENIYVPEKQ 1026

Query: 1027 FHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYMEVTGFSADVREQIYKVASV 1086
            FHSFKK+SRSMGYG+ DI LVSFQSVSKGYYGECGKRGGYMEVTGF+ +VREQIYKVASV
Sbjct: 1031 FHSFKKVSRSMGYGDKDIPLVSFQSVSKGYYGECGKRGGYMEVTGFTPEVREQIYKVASV 1026

Query: 1087 NLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLARRAKTLEAAFNSLENVTCN 1146
            NLCSNISGQILASLVM+PPKVGD  Y+SY AERDGIL SLARRAKTLE A NSLE +TCN
Sbjct: 1091 NLCSNISGQILASLVMSPPKVGDESYDSYFAERDGILQSLARRAKTLEDALNSLEGITCN 1026

Query: 1147 KAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATGIVVVPGSGFGQVPGTWHF 1206
            KAEGAMYLFP I+LP KAIKAAEAA  APDAFY   LLNATGIVVVPGSGFGQVPGTWHF
Sbjct: 1151 KAEGAMYLFPRIQLPNKAIKAAEAAKKAPDAFYAARLLNATGIVVVPGSGFGQVPGTWHF 1026

Query: 1207 RCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            RCTILPQE+KIPA+V++LTDFH+ FM E+RD
Sbjct: 1211 RCTILPQEEKIPAIVTQLTDFHKKFMDEFRD 1026

BLAST of Sgr025362 vs. NCBI nr
Match: XP_022153038.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 993.4 bits (2567), Expect = 1.9e-285
Identity = 513/579 (88.60%), Postives = 541/579 (93.44%), Query Frame = 0

Query: 1   MEKPIVG---ISDSGEQNS-IP--KKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDR 60
           MEKPIV    ISDSG +++ +P  + SKK LLLALSA LL+VGA+VGIVAGVTKSRNS R
Sbjct: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60

Query: 61  --SEAHAILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFR 120
              EAHAI+KS+CS+TLYP+LCFSTIS  +GAMKKVSNQKDVIELSINITVKAVE +YFR
Sbjct: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120

Query: 121 VQKLAALKNITHRERLALHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLS 180
           VQKLAALKNITHRER+ALHDCLETIDETLDELHK+I DLH+YPNKKSL QHADDLKTLLS
Sbjct: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMG 240
           SAITNQETCLDGFSHD+ADKSLRKKLEEGQKEVEHMCSNALAM+KNMTDTDIANY + MG
Sbjct: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240

Query: 241 -SRKLMEEAD-NDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAP 300
            +R L+E AD  D  I WP WLS GDRRLLQSSSVTPD VVAADGSGNFRTVSAAV+AAP
Sbjct: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300

Query: 301 VKSKKRYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
            KSKKRYIIRIKAGVYRENVE+PKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LISGTVDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAA+FQ+CDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTG 540
           DLR VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFY EYQNTG
Sbjct: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540

Query: 541 PGASTSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLR 570
           PGAST+GRVTWKGF+VIRSATEAE FTAGRFIGGG WLR
Sbjct: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLR 579

BLAST of Sgr025362 vs. NCBI nr
Match: XP_004141734.1 (pectinesterase [Cucumis sativus] >KGN45437.1 hypothetical protein Csa_016439 [Cucumis sativus])

HSP 1 Score: 963.0 bits (2488), Expect = 2.7e-276
Identity = 487/578 (84.26%), Postives = 530/578 (91.70%), Query Frame = 0

Query: 1   MEKPI---VGISDSGEQNSIPKKSKKKLLL-ALSATLLLVGAIVGIVAGVTKSRNSDRSE 60
           ME+PI    G+S+S +     K SKKKLL+  L +TLL+VGA++GIVAG TKS +    E
Sbjct: 7   MEQPIAAGAGVSNSSDLQKTQKSSKKKLLIVTLCSTLLIVGAVIGIVAGTTKSSSKYSGE 66

Query: 61  AHAILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKL 120
           +HAI+KS+CSSTLYPD+C+S IS  +GAM KV NQKDVIE+SINITVKAVE +YFRV+KL
Sbjct: 67  SHAIVKSTCSSTLYPDMCYSAISRSEGAMLKVKNQKDVIEISINITVKAVEENYFRVKKL 126

Query: 121 AALKNITHRERLALHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAIT 180
             LKNI+HRER+ALHDCLETIDETLDELHKAI DL+EYPNKKSL QHADDLKTLLSSAIT
Sbjct: 127 CGLKNISHRERIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAIT 186

Query: 181 NQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMG---S 240
           NQETCLDGFSHD+ADK+LR KL+EGQ EVEHMCSNALAM+KNMTDTDIANYE+ MG   +
Sbjct: 187 NQETCLDGFSHDDADKNLRDKLKEGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKN 246

Query: 241 RKLMEEAD---NDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAP 300
           RKLM E D   NDD I+WPEWLSAGDRRLLQSSSVTP+VVVAADGSGNFRTV+AAVAAAP
Sbjct: 247 RKLMAEEDDNNNDDGIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAP 306

Query: 301 VKSKKRYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
           V+S KRY+IRIKAGVYRENVEVPKKKTNIMF+GDGR NTIITGSRNVVDGSTTFNSAT+A
Sbjct: 307 VRSSKRYVIRIKAGVYRENVEVPKKKTNIMFIGDGRRNTIITGSRNVVDGSTTFNSATMA 366

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 367 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 426

Query: 421 LISGTVDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAAIFQ+CDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 427 LVSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 486

Query: 481 DLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTG 540
           DLRPVQK+FPTFLGRPWKEYSRTVIMQ +ISDVIDPKGWHEWSG+FALNTLFYGEYQNTG
Sbjct: 487 DLRPVQKSFPTFLGRPWKEYSRTVIMQCTISDVIDPKGWHEWSGSFALNTLFYGEYQNTG 546

Query: 541 PGASTSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWL 569
            GAST GRVTWKGFRVIRSATEAE+FTAG+FIGGG WL
Sbjct: 547 AGASTGGRVTWKGFRVIRSATEAESFTAGKFIGGGSWL 584

BLAST of Sgr025362 vs. NCBI nr
Match: KAA0059355.1 (pectinesterase-like [Cucumis melo var. makuwa] >TYK03971.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 957.6 bits (2474), Expect = 1.1e-274
Identity = 486/578 (84.08%), Postives = 530/578 (91.70%), Query Frame = 0

Query: 1   MEKPI---VGISDSGEQNSIPKKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDRSEA 60
           ME+PI    GIS+S +     K SKK L+L LS+TLL+VGA++GIVAG TKS + D  ++
Sbjct: 7   MEQPIAAGAGISNSSDLKKNKKASKKLLILTLSSTLLIVGAVIGIVAGTTKS-SKDSGKS 66

Query: 61  HAILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKLA 120
           HAI+KS+CSSTLYPD+C+S IS  +GAM KV +QKDVIE+SINITVKAVE +YFRV+KL 
Sbjct: 67  HAIVKSTCSSTLYPDMCYSAISKSEGAMLKVKSQKDVIEVSINITVKAVEENYFRVKKLC 126

Query: 121 ALKNITHRERLALHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITN 180
            LKNI+HRE++ALHDCLETIDETLDELHKAI DL+EYPNKKSL QHADDLKTLLSSAITN
Sbjct: 127 GLKNISHREKIALHDCLETIDETLDELHKAIVDLNEYPNKKSLNQHADDLKTLLSSAITN 186

Query: 181 QETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMG---SR 240
           QETCLDGFSHD ADK+LR KL+ GQ EVEHMCSNALAM+KNMTDTDIANYE+ MG   +R
Sbjct: 187 QETCLDGFSHDNADKTLRDKLKAGQMEVEHMCSNALAMIKNMTDTDIANYEAKMGITKNR 246

Query: 241 KLM---EEADNDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPV 300
           KLM   E+ +N+D+I+WPEWLSAGDRRLLQSSSVTP+VVVAADGSGNFRTV+AAVAAAP 
Sbjct: 247 KLMAEEEDNNNNDEIQWPEWLSAGDRRLLQSSSVTPNVVVAADGSGNFRTVAAAVAAAPE 306

Query: 301 KSKKRYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAA 360
           KS KRY+IRIKAGVYRENVEVPKKKTNIMF+GDGR NTIITGSRNVVDGSTTF SATVAA
Sbjct: 307 KSSKRYVIRIKAGVYRENVEVPKKKTNIMFMGDGRRNTIITGSRNVVDGSTTFKSATVAA 366

Query: 361 VGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL 420
           VGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL
Sbjct: 367 VGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCL 426

Query: 421 ISGTVDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSD 480
           +SGTVDFIFGNAAAIFQ+CDIHAR+PNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSD
Sbjct: 427 VSGTVDFIFGNAAAIFQDCDIHARKPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSD 486

Query: 481 LRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGP 540
           LRPVQK+FPTFLGRPWKEYSRTVIMQ SISDVIDPKGWHEWSG+FALNTLFYGEYQNTGP
Sbjct: 487 LRPVQKSFPTFLGRPWKEYSRTVIMQCSISDVIDPKGWHEWSGSFALNTLFYGEYQNTGP 546

Query: 541 GASTSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLR 570
           GAST GRVTWKGFRVIRSATEAE FTAG+FIGGG WLR
Sbjct: 547 GASTGGRVTWKGFRVIRSATEAEGFTAGKFIGGGSWLR 583

BLAST of Sgr025362 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 834.3 bits (2154), Expect = 1.9e-240
Identity = 428/557 (76.84%), Postives = 475/557 (85.28%), Query Frame = 0

Query: 15  NSIPKKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNS---DRSEAHAILKSSCSSTLYP 74
           ++IPKK KKKL LAL ATLL+V A++GIVAGV   +NS        HAILKSSCSST YP
Sbjct: 21  SNIPKK-KKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYP 80

Query: 75  DLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKLAALKNITHRERLALH 134
           DLCFS I+A   A KKV++QKDVIE+S+NIT  AVE +YF +QKL    N+T RE++ALH
Sbjct: 81  DLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKREKVALH 140

Query: 135 DCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITNQETCLDGFSHDEAD 194
           DCLETIDETLDELHKA+ DL EYPNKKSL+QHADDLKTL+S+A+TNQ TCLDGFSHD+A+
Sbjct: 141 DCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDAN 200

Query: 195 KSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMGSRKLMEEADNDDDIKWPEW 254
           K +R  L +GQ  VE MCSNALAM+KNMTDTD+     T  +RKL+EE    D   WP W
Sbjct: 201 KHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMM-IMRTSNNRKLIEETSTVDG--WPAW 260

Query: 255 LSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYIIRIKAGVYRENVE 314
           LS GDRRLLQSSSVTP+VVVAADGSGNF+TV+A+VAAAP    KRYIIRIKAGVYRENVE
Sbjct: 261 LSTGDRRLLQSSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYRENVE 320

Query: 315 VPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAGPSK 374
           V KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SATVA VGEGFLARDITFQNTAGPSK
Sbjct: 321 VTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAGPSK 380

Query: 375 HQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAIFQNCD 434
           HQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCLI+GTVDFIFGNAAA+ QNCD
Sbjct: 381 HQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCD 440

Query: 435 IHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGRPWKEYS 494
           IHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+PVQ +FPT+LGRPWKEYS
Sbjct: 441 IHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYS 500

Query: 495 RTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTSGRVTWKGFRVIRSAT 554
           RTVIMQSSI+DVI P GWHEW GNFALNTLFYGE+QN G GA TSGRV WKGFRVI SAT
Sbjct: 501 RTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSAT 560

Query: 555 EAEAFTAGRFIGGGGWL 569
           EA+AFT G FI G  WL
Sbjct: 561 EAQAFTPGSFIAGSSWL 573

BLAST of Sgr025362 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 830.9 bits (2145), Expect = 2.1e-239
Identity = 429/563 (76.20%), Postives = 475/563 (84.37%), Query Frame = 0

Query: 11  SGEQN--SIPKKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNS---DRSEAHAILKSSC 70
           S  QN  +IPKK KKKL LAL ATLL+V A++GIVAGV   +NS        HAILKSSC
Sbjct: 15  SSNQNISNIPKK-KKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSC 74

Query: 71  SSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKLAALKNITHR 130
           SST YPDLCFS I+A   A KKV++QKDVIE+S+NIT  AVE +YF +QKL    N+T R
Sbjct: 75  SSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRTNLTKR 134

Query: 131 ERLALHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITNQETCLDGF 190
           E++ALHDCLETIDETLDELHKA+ DL EYPNKKSL+QHADDLKTL+S+A+TNQ TCLDGF
Sbjct: 135 EKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGF 194

Query: 191 SHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMGSRKLMEEADNDDD 250
           SHD+A+K +R  L +GQ  VE MCSNALAM+KNMTDTD+     T  +RKL EE    D 
Sbjct: 195 SHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMM-IMRTSNNRKLTEETSTVDG 254

Query: 251 IKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYIIRIKAGV 310
             WP WLS GDRRLLQSSSVTP+ VVAADGSGNF+TV+AAVAAAP    KRYIIRIKAGV
Sbjct: 255 --WPAWLSPGDRRLLQSSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGV 314

Query: 311 YRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQN 370
           YRENVEV KK  NIMF+GDGR+ TIITGSRNVVDGSTTF SAT A VGEGFLARDITFQN
Sbjct: 315 YRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQN 374

Query: 371 TAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAA 430
           TAGPSKHQAVALRVGADLSAFY CDMLAYQDTLYVHSNRQF++NCLI+GTVDFIFGNAAA
Sbjct: 375 TAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAA 434

Query: 431 IFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGR 490
           + QNCDIHAR+PNSGQKNMVTAQGR+DPNQNTGIVIQKSRIGATSDL+PVQ +FPT+LGR
Sbjct: 435 VLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGR 494

Query: 491 PWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTSGRVTWKGFR 550
           PWKEYSRTVIMQSSI+D+I P GWHEW GNFALNTLFYGE+QN+G GA TSGRV WKGFR
Sbjct: 495 PWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFR 554

Query: 551 VIRSATEAEAFTAGRFIGGGGWL 569
           VI SATEA+AFT G FI G  WL
Sbjct: 555 VITSATEAQAFTPGSFIAGSSWL 573

BLAST of Sgr025362 vs. ExPASy Swiss-Prot
Match: Q9LDV4 (Alanine aminotransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALAAT2 PE=1 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 6.8e-230
Identity = 387/469 (82.52%), Postives = 429/469 (91.47%), Query Frame = 0

Query: 756  VLKCEYAVRGEIVALAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLAL 815
            VLKCEYAVRGEIV +AQKLQE+L T   +  FDEI+YCNIGNPQSLGQ PI FFREVLAL
Sbjct: 72   VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 816  CDHPSILNRNEIQGLFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARD 875
            CDH S+L+ +E  GLFS D+I++A +IL  IPGRATGAYSHSQGIKGLRD IAAGIEARD
Sbjct: 132  CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 876  GFPADPADLFLTDGASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 935
            GFPADP D+FLTDGASPAVHMMMQLL+SSEKDGIL PIPQYPLYSASIALHGG+LVPYYL
Sbjct: 192  GFPADPNDIFLTDGASPAVHMMMQLLLSSEKDGILSPIPQYPLYSASIALHGGSLVPYYL 251

Query: 936  DEASGWGLEISELKKELESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGL 995
            DEA+GWGLEIS+LKK+LE A+SKGISVRALVVINPGNPTGQVL +ENQ  IV FCKQEGL
Sbjct: 252  DEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEENQRDIVNFCKQEGL 311

Query: 996  VLLADEVYQENIYVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYME 1055
            VLLADEVYQEN+YV DKKFHSFKK++RS+GYGE DISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 312  VLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKGYYGECGKRGGYME 371

Query: 1056 VTGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLAR 1115
            VTGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK GD  Y+SY AERDGILSS+A+
Sbjct: 372  VTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPKPGDDSYDSYMAERDGILSSMAK 431

Query: 1116 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATG 1175
            RAKTLE A NSLE VTCN+AEGAMYLFP I LP+KAI+AAEA  TAPDAFYC+ LLNATG
Sbjct: 432  RAKTLEDALNSLEGVTCNRAEGAMYLFPRINLPQKAIEAAEAEKTAPDAFYCKRLLNATG 491

Query: 1176 IVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            +VVVPGSGFGQVPGTWHFRCTILPQEDKIPA+V+RLT+FH++FM E+R+
Sbjct: 492  VVVVPGSGFGQVPGTWHFRCTILPQEDKIPAIVNRLTEFHKSFMDEFRN 540

BLAST of Sgr025362 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.3e-228
Identity = 408/562 (72.60%), Postives = 473/562 (84.16%), Query Frame = 0

Query: 20  KSKKKLLLALSATLLLVGAIVGIVAGV-TKSRNSD--------RSEAHAILKSSCSSTLY 79
           K KKK+ LA+ A++LLV A++G+VAGV + S+NSD         S AHAI+KS+CS+TL+
Sbjct: 16  KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 75

Query: 80  PDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKL-AALKNITHRERLA 139
           P+LC+S I       KKV++QKDVIELS+NITVKAV  +Y+ V++L    K +T RE++A
Sbjct: 76  PELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 135

Query: 140 LHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITNQETCLDGFSHDE 199
           LHDCLET+DETLDELH A+ DL  YPNKKSL +H +DLKTL+SSAITNQETCLDGFSHDE
Sbjct: 136 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 195

Query: 200 ADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIAN---YESTMGSRKLMEEADNDDDI 259
           ADK +RK L +GQK VE MCSNALAM+ NMTDTDIAN     +   +RKL+E     D+ 
Sbjct: 196 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAPANNRKLVE-----DNG 255

Query: 260 KWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYIIRIKAGVY 319
           +WPEWLSAGDRRLLQSS+VTPDVVVAADGSG+++TVS AV  AP KS KRY+IRIKAGVY
Sbjct: 256 EWPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKRYVIRIKAGVY 315

Query: 320 RENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNT 379
           RENV+VPKKKTNIMF+GDG+SNTIIT SRNV DGSTTF+SATV  V    LARDITFQNT
Sbjct: 316 RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGKVLARDITFQNT 375

Query: 380 AGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAI 439
           AG SKHQAVAL VG+DLSAFY+CDMLAYQDTLYVHSNRQF++ CL++GTVDFIFGN AA+
Sbjct: 376 AGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGTVDFIFGNGAAV 435

Query: 440 FQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGRP 499
           FQ+CDIHARRP SGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDLRPVQK+FPT+LGRP
Sbjct: 436 FQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPVQKSFPTYLGRP 495

Query: 500 WKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTSGRVTWKGFRV 559
           WKEYSRTVIMQSSI+DVI P GWHEW+GNFAL+TLFYGEY NTG GA TSGRV WKG +V
Sbjct: 496 WKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPTSGRVKWKGHKV 555

Query: 560 IRSATEAEAFTAGRFIGGGGWL 569
           I S+TEA+A+T GRFI GG WL
Sbjct: 556 ITSSTEAQAYTPGRFIAGGSWL 572

BLAST of Sgr025362 vs. ExPASy Swiss-Prot
Match: F4I7I0 (Alanine aminotransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=ALAAT1 PE=1 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 2.9e-228
Identity = 382/469 (81.45%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 756  VLKCEYAVRGEIVALAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLAL 815
            V+KCEYAVRGEIV +AQKLQE+L T   +  FDEI+YCNIGNPQSLGQQPITFFREVLAL
Sbjct: 75   VIKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQQPITFFREVLAL 134

Query: 816  CDHPSILNRNEIQGLFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARD 875
            C + ++L+ +   GLFS D+I +A +IL QIPGRATGAYSHSQGIKGLRDAIA GIEARD
Sbjct: 135  CSYTALLDESATHGLFSSDSIERAWKILDQIPGRATGAYSHSQGIKGLRDAIADGIEARD 194

Query: 876  GFPADPADLFLTDGASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 935
            GFPADP D+F+TDGASP VHMMMQLLI+SEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 195  GFPADPNDIFMTDGASPGVHMMMQLLITSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 254

Query: 936  DEASGWGLEISELKKELESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGL 995
            DEASGWGLEISELKK+LE A+SKGI+VRAL VINPGNPTGQVL++ENQ  +V+FCKQEGL
Sbjct: 255  DEASGWGLEISELKKQLEDARSKGITVRALAVINPGNPTGQVLSEENQRDVVKFCKQEGL 314

Query: 996  VLLADEVYQENIYVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYME 1055
            VLLADEVYQEN+YV DKKFHSFKK++RSMGYGE D++LVSFQSVSKGYYGECGKRGGYME
Sbjct: 315  VLLADEVYQENVYVPDKKFHSFKKVARSMGYGEKDLALVSFQSVSKGYYGECGKRGGYME 374

Query: 1056 VTGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLAR 1115
            VTGF++DVREQIYK+ASVNLCSNISGQILASL+M+PPK GD  YESY AE+DGILSSLAR
Sbjct: 375  VTGFTSDVREQIYKMASVNLCSNISGQILASLIMSPPKPGDDSYESYIAEKDGILSSLAR 434

Query: 1116 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATG 1175
            RAKTLE A N LE VTCN+AEGAMYLFPC+ LP+KAI AAEA  TAPD FYC+ LL ATG
Sbjct: 435  RAKTLEEALNKLEGVTCNRAEGAMYLFPCLHLPQKAIAAAEAEKTAPDNFYCKRLLKATG 494

Query: 1176 IVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            IVVVPGSGF QVPGTWHFRCTILPQEDKIPA+V RLT FH++FM E+RD
Sbjct: 495  IVVVPGSGFRQVPGTWHFRCTILPQEDKIPAIVDRLTAFHQSFMDEFRD 543

BLAST of Sgr025362 vs. ExPASy TrEMBL
Match: A0A103XLE1 (Pectinesterase OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_005069 PE=3 SV=1)

HSP 1 Score: 1150.2 bits (2974), Expect = 0.0e+00
Identity = 648/1237 (52.38%), Postives = 802/1237 (64.83%), Query Frame = 0

Query: 7    GISDSGEQNSIPKKSKKKLLLALSATLLLVGAIVG-IVAGVTKSRNSDR------SEAHA 66
            G  D  E+ +  +K++K++L+ + + LLL+  I+G + A +   RN+ +      S +  
Sbjct: 11   GKLDPAEEQAFRRKTRKRILILILSLLLLLALIIGAVTATLLHKRNNHKDASPSSSSSAQ 70

Query: 67   ILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEA--SYFRVQKLA 126
             +K+ CS TLYPD C+S+IS  D      ++ + +++LS+ +   ++ A  S F    + 
Sbjct: 71   SIKAVCSQTLYPDSCYSSISQLD--QSNSTDPEHLLKLSLQVVFNSLSALSSSFPHSFIK 130

Query: 127  ALKNITHRERLALHDCLETIDETLDELHKAIA--DLHEYPNKKSLTQHADDLKTLLSSAI 186
            +  N T ++  AL  C   +D+ LD L  +I+  DL    +K       +DLKT LS+A+
Sbjct: 131  STTNDTLKQ--ALQVCATLLDDALDYLRDSISTMDLKSADDKLLTLTKIEDLKTWLSTAL 190

Query: 187  TNQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMGSRK 246
            TNQ+TCLD     +   +L    +   +   H  SN+LA+V  +  + I    +    RK
Sbjct: 191  TNQDTCLDALQEIDPSSTLLADTKSLMQNSTHYASNSLAIVSKI--SGILRKLNLPMHRK 250

Query: 247  LMEEADNDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKK 306
            L+ E        +PEW+S G RRLLQ    TP+V VAADG+G+  T+  A+A  P KSK 
Sbjct: 251  LLGE--------FPEWVSPGVRRLLQVGRPTPNVTVAADGTGDVTTIKEAMARVPKKSKT 310

Query: 307  RYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEG 366
             +II IK GVY ENV + K   N+M  GDG+  +I++   N +DG  TF++AT A  G G
Sbjct: 311  MFIIHIKEGVYLENVVLDKSFWNVMIYGDGKDKSIVSARLNFIDGIPTFSTATFAVAGRG 370

Query: 367  FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGT 426
            F+A D+ F+NTAG  KHQAVA R G+D S  Y+C   A+QDTLY HSNRQFY +C ++GT
Sbjct: 371  FVAIDMGFKNTAGAEKHQAVAFRSGSDFSVLYRCSFDAFQDTLYPHSNRQFYRDCDVTGT 430

Query: 427  VDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPV 486
            +DFIFGNAA +FQNC I  R+P   Q   +TAQG+ DPNQNTGI IQK  I    +L   
Sbjct: 431  IDFIFGNAAVVFQNCKIMPRQPLPRQFVTITAQGKKDPNQNTGISIQKCDISPFDNL--- 490

Query: 487  QKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEW-SGNFALNTLFYGEYQNTGPGAS 546
                PT+LGRPWK++S TVIMQS+I   + P GW  W  G     ++ YGEY NTG GA+
Sbjct: 491  --TAPTYLGRPWKDFSTTVIMQSNIGSFLHPSGWISWVQGVDPPKSILYGEYLNTGAGAA 550

Query: 547  TSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLRGKKQEKKKNFAPPSPEIENRHNKNR 606
               RVTW G++ I + +EA  FT   FI          +++ ++F               
Sbjct: 551  VDKRVTWDGYKAILTDSEAGRFTVDSFI----------EDRIRSF--------------- 610

Query: 607  HSKSARCSGVEQTGMGINGGRGKLKDAMQWNCMSHFNVRLRDLVSVDSDKGRCVQLVLSL 666
                                                                CV  +  +
Sbjct: 611  ----------------------------------------------------CVAFLPPI 670

Query: 667  SFLVIRFFDLSYGASFRRLPSGFHSDHQPQVIVVDILVIVPIPYSFRVFAFCAGVWITSF 726
            S   +    L    + R+  +     H+ + I+      +  P+     +       +S 
Sbjct: 671  SPTDV----LKTNTAMRKFVA-----HRAKTIITKATASLFTPHRIHPTS-------SSS 730

Query: 727  GCSLPSVYMYSSPESGR-----ESGCSIRWKMTTPGYFETRFLHFMVLKCEYAVRGEIVA 786
              S    Y Y +    R     +S  S+    + P       L+  VLKCEYAVRGEIV+
Sbjct: 731  SSSSDFYYYYYTRTLNRFFTSLDSSTSMSSDKSAPP-LTVESLNPKVLKCEYAVRGEIVS 790

Query: 787  LAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSILNRNEIQG 846
            LAQKLQ++L   PGS  F+EILYCNIGNPQSLGQQPITFFREVLALCDHP+IL+++E QG
Sbjct: 791  LAQKLQQDLQANPGSHPFEEILYCNIGNPQSLGQQPITFFREVLALCDHPTILDKSETQG 850

Query: 847  LFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFPADPADLFLTDG 906
            LFS D+I +A QIL QIPGRATGAYSHSQGIKGLRDAIAAGIEARDGF ADP D+FLTDG
Sbjct: 851  LFSADSIERAWQILDQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFSADPNDIFLTDG 910

Query: 907  ASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTL--VPYYLDEASGWGLEISE 966
            ASPAVHMMMQLLI SEKDGI CPIPQYPLYSASIALHGGTL  VPYYLDEA+GWGLE+SE
Sbjct: 911  ASPAVHMMMQLLIRSEKDGIFCPIPQYPLYSASIALHGGTLVCVPYYLDEATGWGLEVSE 970

Query: 967  LKKELESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGLVLLADEVYQENI 1026
            LKK+LE A+  GI+VRALVVINPGNPTGQVL +ENQ  IVEFCK+EGLVLLADEVYQENI
Sbjct: 971  LKKQLEIARQNGITVRALVVINPGNPTGQVLAEENQRDIVEFCKKEGLVLLADEVYQENI 1030

Query: 1027 YVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYMEVTGFSADVREQI 1086
            Y  +K+FHSFKK+SRSMGYG+ DI LVSFQSVSKGYYGECGKRGGYMEVTGFS +VREQI
Sbjct: 1031 YAPNKQFHSFKKVSRSMGYGDKDIPLVSFQSVSKGYYGECGKRGGYMEVTGFSPEVREQI 1090

Query: 1087 YKVASVNLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLARRAKTLEAAFNSL 1146
            YKVASVNLCSNISGQILASLVM+PPKVGD  Y+SY AERDGIL SLARRAKTLE A NSL
Sbjct: 1091 YKVASVNLCSNISGQILASLVMSPPKVGDESYDSYFAERDGILQSLARRAKTLEDALNSL 1134

Query: 1147 ENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATGIVVVPGSGFGQV 1206
            E V+CNKAEGAMYLFP I+LP KAIK AEAA  APDA+Y   LLNATGIVVVPGSGFGQV
Sbjct: 1151 EGVSCNKAEGAMYLFPRIRLPNKAIKEAEAAKKAPDAYYASRLLNATGIVVVPGSGFGQV 1134

Query: 1207 PGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            PGTWHFRCTILPQE+KIPA+VSRLT+FH+ FM E+RD
Sbjct: 1211 PGTWHFRCTILPQEEKIPAIVSRLTEFHKKFMDEFRD 1134

BLAST of Sgr025362 vs. ExPASy TrEMBL
Match: A0A2U1NZ86 (Pectinesterase OS=Artemisia annua OX=35608 GN=CTI12_AA211570 PE=3 SV=1)

HSP 1 Score: 1109.7 bits (2869), Expect = 0.0e+00
Identity = 622/1231 (50.53%), Postives = 769/1231 (62.47%), Query Frame = 0

Query: 7    GISDSGEQNSIPKKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDRSEAHA------- 66
            G  D  E+ +  +K++K+L++ + + +LLV  I+G V GV  SR++    A +       
Sbjct: 11   GKVDPVEEQAFRRKTRKRLVILIVSVVLLVCVIIGAVVGVIHSRSNGNDSADSSGSSSSS 70

Query: 67   --ILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAV-EASYFRVQKL 126
               +K+ CS T YP+ C++++S  D      ++ +++++LS+ +   ++ E S F     
Sbjct: 71   AQSIKAVCSQTRYPESCYTSLS--DINKSNSTDPEELLKLSLEVVYNSLSELSLFPKNME 130

Query: 127  AALKNITHRERLALHDCLETIDETLDELHKAIADLHEYPNKKSLT-QHADDLKTLLSSAI 186
               K++  ++  AL  C   +D+ +D L  +++ +      K L+    DDLKT LS+AI
Sbjct: 131  NLTKDVNVKK--ALQVCGIVLDDAVDYLRDSMSSMDVKSGDKLLSVTKIDDLKTWLSTAI 190

Query: 187  TNQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMGSRK 246
            TNQETCLD  S DE + +    ++   +      SN+LA+V  +  + I    +    RK
Sbjct: 191  TNQETCLD--SLDEMNATFIDDIKLKMQNSSEYASNSLAIVSKI--SGILGKFNLPIHRK 250

Query: 247  LMEEADNDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKK 306
            L+E     +   +P W++ G RRLLQ+   TP+V VA DG+G+  T+  A+A  P KSK 
Sbjct: 251  LLETGSVSE---FPAWVNPGVRRLLQTGRPTPNVTVATDGTGDVTTLKEAMAKVPKKSKT 310

Query: 307  RYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEG 366
            R+II IK G YRENV + K   N+M  GDG++ +++ G  N VDG+ TF++AT A  G G
Sbjct: 311  RFIIYIKTGKYRENVVLDKSFWNVMIYGDGKTKSVVVGRLNFVDGTPTFSTATFAVQGRG 370

Query: 367  FLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGT 426
            F+A D+ F N AG  KHQAVA R G+D S FY+C   A+QDTLY HSNRQFY  C ++GT
Sbjct: 371  FVAMDMGFINEAGAEKHQAVAFRSGSDFSVFYRCSFDAFQDTLYPHSNRQFYRECDVTGT 430

Query: 427  VDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPV 486
            +DFIFGNAA +FQ+C I  R+P   Q   +TAQG+ DPNQNTGI IQK       D+ P 
Sbjct: 431  IDFIFGNAAVVFQSCKIMPRQPLKNQFVTITAQGKKDPNQNTGISIQK------CDITPF 490

Query: 487  QK-NFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEW-SGNFALNTLFYGEYQNTGPGA 546
                 PT+LGRPWKE+S TVIMQS+I   ++P GW  W SG     +++YGEY NTG GA
Sbjct: 491  DALTAPTYLGRPWKEFSTTVIMQSTIGSFLNPVGWISWVSGVDPPASIYYGEYLNTGLGA 550

Query: 547  STSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLRGKKQEKKKNFAPPSPEIENRHNKN 606
            S + RV+W G++   ++++A  FT                                    
Sbjct: 551  SVAKRVSWAGYKPSMTSSDAGKFT------------------------------------ 610

Query: 607  RHSKSARCSGVEQTGMGINGGRGKLKDAMQWNCMSHFNVRLRDLVSVDSDKGRCVQLVLS 666
                                                                        
Sbjct: 611  ------------------------------------------------------------ 670

Query: 667  LSFLVIRFFDLSYGASFRRLPSGFHSDHQPQVIVVDILVIVPIPYSFRVFAFCAGVWITS 726
                                                                        
Sbjct: 671  ------------------------------------------------------------ 730

Query: 727  FGCSLPSVYMYSSPESGRESGCSIRWKMTTPGYFETRFLHFMVLKCEYAVRGEIVALAQK 786
                                                      VLKCEYAVRGEIV+LAQ 
Sbjct: 731  ------------------------------------------VLKCEYAVRGEIVSLAQN 790

Query: 787  LQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSILNRNEIQGLFSE 846
            LQ++L   PG+  F+EI+YCNIGNPQSLGQQPITFFREVLALCDHP+IL+++E QGLFS 
Sbjct: 791  LQQDLKANPGTHPFEEIIYCNIGNPQSLGQQPITFFREVLALCDHPTILDKSETQGLFSA 850

Query: 847  DAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFPADPADLFLTDGASPA 906
            D+IN+A +IL QIPGRATGAYSHSQG+KGLRD IAAGIEARDGFPA+P D+FLTDGASPA
Sbjct: 851  DSINRAWEILDQIPGRATGAYSHSQGVKGLRDTIAAGIEARDGFPANPDDIFLTDGASPA 910

Query: 907  VHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYLDEASGWGLEISELKKELE 966
            VHMMMQLLI SE DGI CPIPQYPLYSASIALHGGTLVPYYLDEA+GWGLEISELKK+LE
Sbjct: 911  VHMMMQLLIRSESDGIFCPIPQYPLYSASIALHGGTLVPYYLDEATGWGLEISELKKQLE 970

Query: 967  SAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGLVLLADEVYQENIYVRDKK 1026
            +A+SKGI+VRALVVINPGNPTGQVL +ENQ  IVEFCK+EGLVLLADEVYQENIYV +K+
Sbjct: 971  TARSKGITVRALVVINPGNPTGQVLAEENQRDIVEFCKKEGLVLLADEVYQENIYVPEKQ 1026

Query: 1027 FHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYMEVTGFSADVREQIYKVASV 1086
            FHSFKK+SRSMGYG+ DI LVSFQSVSKGYYGECGKRGGYMEVTGF+ +VREQIYKVASV
Sbjct: 1031 FHSFKKVSRSMGYGDKDIPLVSFQSVSKGYYGECGKRGGYMEVTGFTPEVREQIYKVASV 1026

Query: 1087 NLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLARRAKTLEAAFNSLENVTCN 1146
            NLCSNISGQILASLVM+PPKVGD  Y+SY AERDGIL SLARRAKTLE A NSLE +TCN
Sbjct: 1091 NLCSNISGQILASLVMSPPKVGDESYDSYFAERDGILQSLARRAKTLEDALNSLEGITCN 1026

Query: 1147 KAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATGIVVVPGSGFGQVPGTWHF 1206
            KAEGAMYLFP I+LP KAIKAAEAA  APDAFY   LLNATGIVVVPGSGFGQVPGTWHF
Sbjct: 1151 KAEGAMYLFPRIQLPNKAIKAAEAAKKAPDAFYAARLLNATGIVVVPGSGFGQVPGTWHF 1026

Query: 1207 RCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            RCTILPQE+KIPA+V++LTDFH+ FM E+RD
Sbjct: 1211 RCTILPQEEKIPAIVTQLTDFHKKFMDEFRD 1026

BLAST of Sgr025362 vs. ExPASy TrEMBL
Match: A0A4D8Z6R6 (Pectinesterase OS=Salvia splendens OX=180675 GN=GPT PE=3 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 9.0e-302
Identity = 621/1217 (51.03%), Postives = 719/1217 (59.08%), Query Frame = 0

Query: 11   SGEQNSIPKKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDRSEAHAILKSSCSSTLY 70
            +G+  S  K +++KLL  L+A +L    I G  AG   S     S AH + +SSCSSTLY
Sbjct: 9    AGKPFSGDKTTRQKLLPLLAAIILASAVIGGAAAG--DSATLKISAAHTLARSSCSSTLY 68

Query: 71   PDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKLAA--LKNITHRERL 130
            P+LC+S I+A     KKV + KDVI LS+N+T+ AV+ +Y  + KL A    N T RE+L
Sbjct: 69   PELCYSAIAASIDGGKKVESGKDVIFLSLNVTIAAVQRNYAAIGKLIADNKSNFTKREKL 128

Query: 131  ALHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITNQETCLDGFSHD 190
            AL+DCLET+DETLDEL  A+ +L+EYP KKS+  HADDLKTLLSSAITNQETCLDGFS+ 
Sbjct: 129  ALNDCLETLDETLDELETAVKELNEYPFKKSIEAHADDLKTLLSSAITNQETCLDGFSYS 188

Query: 191  EADKSLRKKLEEGQ-KEVEHMCSNALAMVKNMTDTDIANYESTMGSRKLMEEADNDDDIK 250
            E +K +R+    GQ   VE +CSN+LAM+ NMT  DI         R+L E         
Sbjct: 189  ETEKHVREVFIGGQVVRVEKLCSNSLAMLTNMTRKDIDKERKLNRGRRLAEGG------- 248

Query: 251  WPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYIIRIKAGVYR 310
            WPEWLSAGDRRLLQSS+VTPDVVVAADGSG+++TV+ AVAAAP KSK RY+IRIKAGVY+
Sbjct: 249  WPEWLSAGDRRLLQSSTVTPDVVVAADGSGDYKTVAEAVAAAPEKSKTRYVIRIKAGVYK 308

Query: 311  ENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTA 370
            ENVEV KKKTNIMFVGDGR+ TIIT S+NVVDGSTTF SATV  +               
Sbjct: 309  ENVEVSKKKTNIMFVGDGRTTTIITASKNVVDGSTTFKSATVGEL--------------- 368

Query: 371  GPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAIF 430
                                                 +F + CL+      +   A   F
Sbjct: 369  -------------------------------------RFLLFCLL------VVSRAHRKF 428

Query: 431  QNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLGRPW 490
                               A G   P  ++ +V   S                       
Sbjct: 429  ------------------LAGGLQHPTASSSLVSSHS----------------------- 488

Query: 491  KEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTSGRVTWKGFRVI 550
                                                  + N  P +S           ++
Sbjct: 489  --------------------------------------HHNLLPYSSL----------LL 548

Query: 551  RSATEAEAFTAGRFIGGGGWLRGKKQEKKKNFAPPSPEIENRHNKNRHSKSARCSGVEQT 610
            R  T   A                         PP+P                       
Sbjct: 549  RLLTTTAA------------------------NPPAPS---------------------- 608

Query: 611  GMGINGGRGKLKDAMQWNCMSHFNVRLRDLVSVDSDKGRCVQLVLSLSFLVIRFFDLSYG 670
                                                                        
Sbjct: 609  ------------------------------------------------------------ 668

Query: 671  ASFRRLPSGFHSDHQPQVIVVDILVIVPIPYSFRVFAFCAGVWITSFGCSLPSVYMYSSP 730
                       SD+    + +D                                      
Sbjct: 669  -------ESMSSDYSSTPVKID-------------------------------------- 728

Query: 731  ESGRESGCSIRWKMTTPGYFETRFLHFMVLKCEYAVRGEIVALAQKLQEELLTKPGSRSF 790
                    SI  K               VL+CEYAVRGEIV LAQKLQE+L   P S  F
Sbjct: 729  --------SINPK---------------VLECEYAVRGEIVNLAQKLQEDLKVNPDSHPF 788

Query: 791  DEILYCNIGNPQSLGQQPITFFREVLALCDHPSILNRNEIQGLFSEDAINQALQILKQIP 850
            DEI+YCNIGNPQSLGQQPITFFREVLALCDHP+IL++ E QG+FS D+I +A QIL QIP
Sbjct: 789  DEIIYCNIGNPQSLGQQPITFFREVLALCDHPAILDKGETQGIFSADSIERAFQILDQIP 848

Query: 851  GRATGAYSHSQGIKGLRDAIAAGIEARDGFPADPADLFLTDGASPAVHMMMQLLISSEKD 910
            GRATGAYSHSQGIKGLRD I AGIEARDGFPADP D+FLTDGASPAVHMMMQLLI S+ D
Sbjct: 849  GRATGAYSHSQGIKGLRDTICAGIEARDGFPADPNDIFLTDGASPAVHMMMQLLIRSKND 895

Query: 911  GILCPIPQYPLYSASIALHGGTLVPYYLDEASGWGLEISELKKELESAKSKGISVRALVV 970
            GILCPIPQYPLYSASIALHGG+LVPYYLDEA+GWGLE+S++KK+LE+A+SKGI VRALVV
Sbjct: 909  GILCPIPQYPLYSASIALHGGSLVPYYLDEATGWGLEVSDVKKQLETARSKGIDVRALVV 895

Query: 971  INPGNPTGQVLTKENQEQIVEFCKQEGLVLLADEVYQENIYVRDKKFHSFKKISRSMGYG 1030
            INPGNPTGQVL + NQ+QIVEFCK+EGLVLLADEVYQEN+YV DK FHSFKK++RSMGYG
Sbjct: 969  INPGNPTGQVLAESNQQQIVEFCKKEGLVLLADEVYQENVYVPDKHFHSFKKVARSMGYG 895

Query: 1031 ENDISLVSFQSVSKGYYGECGKRGGYMEVTGFSADVREQIYKVASVNLCSNISGQILASL 1090
            E DISLVSFQSVSKGYYGECGKRGGYMEVTGF+ ++REQ+YK+ASVNLCSNISGQILASL
Sbjct: 1029 EKDISLVSFQSVSKGYYGECGKRGGYMEVTGFAPEIREQMYKLASVNLCSNISGQILASL 895

Query: 1091 VMNPPKVGDRIYESYCAERDGILSSLARRAKTLEAAFNSLENVTCNKAEGAMYLFPCIKL 1150
            VM+PPKVGD  YESY AERD ILSSLARRAKTLE A NSLE VTCN+AEGAMYLFP I L
Sbjct: 1089 VMSPPKVGDESYESYTAERDAILSSLARRAKTLEDALNSLEGVTCNRAEGAMYLFPRITL 895

Query: 1151 PEKAIKAAEAANTAPDAFYCRHLLNATGIVVVPGSGFGQVPGTWHFRCTILPQEDKIPAV 1210
            P KAI+AA+A  TAPDAFY R LLNATG+VVVPGSGFGQVPGTWHFRCTILPQED+IPA+
Sbjct: 1149 PNKAIEAAKAVKTAPDAFYARRLLNATGVVVVPGSGFGQVPGTWHFRCTILPQEDRIPAI 895

Query: 1211 VSRLTDFHRAFMYEYRD 1225
            VSRLT+FH+AFM EYRD
Sbjct: 1209 VSRLTEFHKAFMNEYRD 895

BLAST of Sgr025362 vs. ExPASy TrEMBL
Match: A0A6N2K5N8 (Pectinesterase OS=Salix viminalis OX=40686 GN=SVIM_LOCUS32617 PE=4 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 1.3e-287
Identity = 554/981 (56.47%), Postives = 621/981 (63.30%), Query Frame = 0

Query: 249  PEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYIIRIKAGVYRE 308
            P W+  GD+RLL+SSS   DV V  D SGN  T+S AV   P KSK R+II +K G Y E
Sbjct: 45   PHWVGLGDKRLLESSSPKLDVTVVKDDSGNCTTLSEAVGKIPKKSKSRFIIYVKDGEYLE 104

Query: 309  NVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQNTAG 368
            NV + K K N+M  G+G+  TII+GS N VDG     S TVA  G+GF ARDI F NTAG
Sbjct: 105  NVILDKSKWNVMIYGEGKDKTIISGSLNFVDGG---ESPTVA--GKGFFARDIKFINTAG 164

Query: 369  PSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAAAIFQ 428
              KHQAVA R G+D+S  ++C    +QDTLY HSNRQFY +C I+GTVDFI         
Sbjct: 165  AEKHQAVAFRSGSDMSVLFRCAFHGFQDTLYTHSNRQFYRDCDITGTVDFI--------- 224

Query: 429  NCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIG-ATSDLRPVQKNF--PTFLGR 488
                 +  P S  K   ++ G   P  +T   +++ RI   T   +    NF  PT+LGR
Sbjct: 225  -----SAMPPSCSKTATSSPGNRRPTSSTPSQLRERRIRIKTPAFQSRTVNFLPPTYLGR 284

Query: 489  PWKEYS-RTVIMQSSISDVIDPKGWHEW-SGNFALNTLFYGEYQNTGPGASTSGRVTWKG 548
            PWK+YS  TVIMQS I   +   GW  W SG     TLFY EYQNTGP A+  GRV W G
Sbjct: 285  PWKDYSTTTVIMQSDIGPFLRAPGWISWVSGVDPPATLFYAEYQNTGPAANVDGRVRWAG 344

Query: 549  FRVIRSATEAEAFTAGRFIGGGGWLRGKKQEKKKNFAPPSPEIENRHNKNRHSKSARCSG 608
            +R   +  EAE FT G FI G  WL                                   
Sbjct: 345  YRPALTVDEAERFTVGSFIRGSEWL----------------------------------- 404

Query: 609  VEQTGMGINGGRGKLKDAMQWNCMSHFNVRLRDLVSVDSDKGRCVQLVLSLSFLVIRFFD 668
                                                                        
Sbjct: 405  ------------------------------------------------------------ 464

Query: 669  LSYGASFRRLPSGFHSDHQPQVIVVDILVIVPIPYSFRVFAFCAGVWITSFGCSLPSVYM 728
                                                                        
Sbjct: 465  ------------------------------------------------------------ 524

Query: 729  YSSPESGRESGCSIRWKMTTPGYFETRFLHFMVLKCEYAVRGEIVALAQKLQEELLTKPG 788
                              TT            VLKCEYAVRGEIV LAQ LQEEL +KPG
Sbjct: 525  ----------------PATT------------VLKCEYAVRGEIVTLAQALQEELKSKPG 584

Query: 789  SRSFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSILNRNEIQGLFSEDAINQALQIL 848
            S SFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSIL++++ + LFS DAI +A QIL
Sbjct: 585  SHSFDEILYCNIGNPQSLGQQPITFFREVLALCDHPSILDKSQTRSLFSADAIKRARQIL 644

Query: 849  KQIPGRATGAYSHSQGIKGLRDAIAAGIEARDGFPADPADLFLTDGASPAVHMMMQLLIS 908
             QIPGRATGAYSHSQGIKGLRD IAAGIEARDGFPADP D+FLTDGASPAVH+MMQLLI 
Sbjct: 645  DQIPGRATGAYSHSQGIKGLRDTIAAGIEARDGFPADPNDIFLTDGASPAVHLMMQLLIR 704

Query: 909  SEKDGILCPIPQYPLYSASIALHGGTLVPYYLDEASGWGLEISELKKELESAKSKGISVR 968
            SEKDGILCPIPQYPLYSASIAL GGTLVPYYLDEA+GWGLE+SELKK+   AKSKGI+ R
Sbjct: 705  SEKDGILCPIPQYPLYSASIALRGGTLVPYYLDEAAGWGLEVSELKKQWADAKSKGITPR 764

Query: 969  ALVVINPGNPTGQVLTKENQEQIVEFCKQEGLVLLADEVYQENIYVRDKKFHSFKKISRS 1028
            ALVVINPGNPTGQVL + NQ +IV+FCK+EGLVLLADEVYQEN+YV +KKFHSFKK++RS
Sbjct: 765  ALVVINPGNPTGQVLAENNQREIVDFCKKEGLVLLADEVYQENVYVPEKKFHSFKKVARS 823

Query: 1029 MGYGENDISLVSFQSVSKGYYGECGKRGGYMEVTGFSADVREQIYKVASVNLCSNISGQI 1088
            MGYGE DIS+VSFQSVSKGYYGECGKRGGYMEVTGF  ++REQIYK+ASVNLCSNISGQI
Sbjct: 825  MGYGEKDISVVSFQSVSKGYYGECGKRGGYMEVTGFGPEIREQIYKLASVNLCSNISGQI 823

Query: 1089 LASLVMNPPKVGDRIYESYCAERDGILSSLARRAKTLEAAFNSLENVTCNKAEGAMYLFP 1148
            L+SLVM+PPKVGD  YES+ AE+DGILSSLARRAKTLE AFN+LE VTCNKAEGAMYLFP
Sbjct: 885  LSSLVMSPPKVGDESYESFSAEKDGILSSLARRAKTLEDAFNTLEGVTCNKAEGAMYLFP 823

Query: 1149 CIKLPEKAIKAAEAANTAPDAFYCRHLLNATGIVVVPGSGFGQVPGTWHFRCTILPQEDK 1208
            CI LPEKAIKAAEA  T PD FYCR LLNATGIV VPGSGF Q PGTWH RCTILPQEDK
Sbjct: 945  CISLPEKAIKAAEAVKTTPDNFYCRRLLNATGIVFVPGSGFRQAPGTWHCRCTILPQEDK 823

Query: 1209 IPAVVSRLTDFHRAFMYEYRD 1225
            IPAVVSRLT+FHR+FM E+R+
Sbjct: 1005 IPAVVSRLTEFHRSFMEEFRE 823

BLAST of Sgr025362 vs. ExPASy TrEMBL
Match: A0A6J1DGG5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111020634 PE=3 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 9.1e-286
Identity = 513/579 (88.60%), Postives = 541/579 (93.44%), Query Frame = 0

Query: 1   MEKPIVG---ISDSGEQNS-IP--KKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDR 60
           MEKPIV    ISDSG +++ +P  + SKK LLLALSA LL+VGA+VGIVAGVTKSRNS R
Sbjct: 1   MEKPIVAGMEISDSGHRSTPVPRNRNSKKTLLLALSAALLVVGAVVGIVAGVTKSRNSGR 60

Query: 61  --SEAHAILKSSCSSTLYPDLCFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFR 120
              EAHAI+KS+CS+TLYP+LCFSTIS  +GAMKKVSNQKDVIELSINITVKAVE +YFR
Sbjct: 61  QSEEAHAIVKSTCSATLYPELCFSTISRSEGAMKKVSNQKDVIELSINITVKAVEENYFR 120

Query: 121 VQKLAALKNITHRERLALHDCLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLS 180
           VQKLAALKNITHRER+ALHDCLETIDETLDELHK+I DLH+YPNKKSL QHADDLKTLLS
Sbjct: 121 VQKLAALKNITHRERIALHDCLETIDETLDELHKSIVDLHQYPNKKSLDQHADDLKTLLS 180

Query: 181 SAITNQETCLDGFSHDEADKSLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYESTMG 240
           SAITNQETCLDGFSHD+ADKSLRKKLEEGQKEVEHMCSNALAM+KNMTDTDIANY + MG
Sbjct: 181 SAITNQETCLDGFSHDDADKSLRKKLEEGQKEVEHMCSNALAMIKNMTDTDIANYHAKMG 240

Query: 241 -SRKLMEEAD-NDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAP 300
            +R L+E AD  D  I WP WLS GDRRLLQSSSVTPD VVAADGSGNFRTVSAAV+AAP
Sbjct: 241 ANRNLIENADAGDAAIIWPHWLSPGDRRLLQSSSVTPDAVVAADGSGNFRTVSAAVSAAP 300

Query: 301 VKSKKRYIIRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360
            KSKKRYIIRIKAGVYRENVE+PKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA
Sbjct: 301 DKSKKRYIIRIKAGVYRENVEIPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVA 360

Query: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420
           AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC
Sbjct: 361 AVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINC 420

Query: 421 LISGTVDFIFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480
           L+SGTVDFIFGNAAA+FQ+CDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS
Sbjct: 421 LVSGTVDFIFGNAAAVFQDCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATS 480

Query: 481 DLRPVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTG 540
           DLR VQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFY EYQNTG
Sbjct: 481 DLRSVQKNFPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYAEYQNTG 540

Query: 541 PGASTSGRVTWKGFRVIRSATEAEAFTAGRFIGGGGWLR 570
           PGAST+GRVTWKGF+VIRSATEAE FTAGRFIGGG WLR
Sbjct: 541 PGASTAGRVTWKGFKVIRSATEAEPFTAGRFIGGGSWLR 579

BLAST of Sgr025362 vs. TAIR 10
Match: AT1G72330.1 (alanine aminotransferase 2 )

HSP 1 Score: 799.3 bits (2063), Expect = 4.8e-231
Identity = 387/469 (82.52%), Postives = 429/469 (91.47%), Query Frame = 0

Query: 756  VLKCEYAVRGEIVALAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLAL 815
            VLKCEYAVRGEIV +AQKLQE+L T   +  FDEI+YCNIGNPQSLGQ PI FFREVLAL
Sbjct: 72   VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 816  CDHPSILNRNEIQGLFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARD 875
            CDH S+L+ +E  GLFS D+I++A +IL  IPGRATGAYSHSQGIKGLRD IAAGIEARD
Sbjct: 132  CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 876  GFPADPADLFLTDGASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 935
            GFPADP D+FLTDGASPAVHMMMQLL+SSEKDGIL PIPQYPLYSASIALHGG+LVPYYL
Sbjct: 192  GFPADPNDIFLTDGASPAVHMMMQLLLSSEKDGILSPIPQYPLYSASIALHGGSLVPYYL 251

Query: 936  DEASGWGLEISELKKELESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGL 995
            DEA+GWGLEIS+LKK+LE A+SKGISVRALVVINPGNPTGQVL +ENQ  IV FCKQEGL
Sbjct: 252  DEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEENQRDIVNFCKQEGL 311

Query: 996  VLLADEVYQENIYVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYME 1055
            VLLADEVYQEN+YV DKKFHSFKK++RS+GYGE DISLVSFQSVSKGYYGECGKRGGYME
Sbjct: 312  VLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKGYYGECGKRGGYME 371

Query: 1056 VTGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLAR 1115
            VTGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK GD  Y+SY AERDGILSS+A+
Sbjct: 372  VTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPKPGDDSYDSYMAERDGILSSMAK 431

Query: 1116 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATG 1175
            RAKTLE A NSLE VTCN+AEGAMYLFP I LP+KAI+AAEA  TAPDAFYC+ LLNATG
Sbjct: 432  RAKTLEDALNSLEGVTCNRAEGAMYLFPRINLPQKAIEAAEAEKTAPDAFYCKRLLNATG 491

Query: 1176 IVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            +VVVPGSGFGQVPGTWHFRCTILPQEDKIPA+V+RLT+FH++FM E+R+
Sbjct: 492  VVVVPGSGFGQVPGTWHFRCTILPQEDKIPAIVNRLTEFHKSFMDEFRN 540

BLAST of Sgr025362 vs. TAIR 10
Match: AT1G17290.1 (alanine aminotransferas )

HSP 1 Score: 793.9 bits (2049), Expect = 2.0e-229
Identity = 382/469 (81.45%), Postives = 424/469 (90.41%), Query Frame = 0

Query: 756  VLKCEYAVRGEIVALAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLAL 815
            V+KCEYAVRGEIV +AQKLQE+L T   +  FDEI+YCNIGNPQSLGQQPITFFREVLAL
Sbjct: 75   VIKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQQPITFFREVLAL 134

Query: 816  CDHPSILNRNEIQGLFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARD 875
            C + ++L+ +   GLFS D+I +A +IL QIPGRATGAYSHSQGIKGLRDAIA GIEARD
Sbjct: 135  CSYTALLDESATHGLFSSDSIERAWKILDQIPGRATGAYSHSQGIKGLRDAIADGIEARD 194

Query: 876  GFPADPADLFLTDGASPAVHMMMQLLISSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 935
            GFPADP D+F+TDGASP VHMMMQLLI+SEKDGILCPIPQYPLYSASIALHGGTLVPYYL
Sbjct: 195  GFPADPNDIFMTDGASPGVHMMMQLLITSEKDGILCPIPQYPLYSASIALHGGTLVPYYL 254

Query: 936  DEASGWGLEISELKKELESAKSKGISVRALVVINPGNPTGQVLTKENQEQIVEFCKQEGL 995
            DEASGWGLEISELKK+LE A+SKGI+VRAL VINPGNPTGQVL++ENQ  +V+FCKQEGL
Sbjct: 255  DEASGWGLEISELKKQLEDARSKGITVRALAVINPGNPTGQVLSEENQRDVVKFCKQEGL 314

Query: 996  VLLADEVYQENIYVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKGYYGECGKRGGYME 1055
            VLLADEVYQEN+YV DKKFHSFKK++RSMGYGE D++LVSFQSVSKGYYGECGKRGGYME
Sbjct: 315  VLLADEVYQENVYVPDKKFHSFKKVARSMGYGEKDLALVSFQSVSKGYYGECGKRGGYME 374

Query: 1056 VTGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDRIYESYCAERDGILSSLAR 1115
            VTGF++DVREQIYK+ASVNLCSNISGQILASL+M+PPK GD  YESY AE+DGILSSLAR
Sbjct: 375  VTGFTSDVREQIYKMASVNLCSNISGQILASLIMSPPKPGDDSYESYIAEKDGILSSLAR 434

Query: 1116 RAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAPDAFYCRHLLNATG 1175
            RAKTLE A N LE VTCN+AEGAMYLFPC+ LP+KAI AAEA  TAPD FYC+ LL ATG
Sbjct: 435  RAKTLEEALNKLEGVTCNRAEGAMYLFPCLHLPQKAIAAAEAEKTAPDNFYCKRLLKATG 494

Query: 1176 IVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEYRD 1225
            IVVVPGSGF QVPGTWHFRCTILPQEDKIPA+V RLT FH++FM E+RD
Sbjct: 495  IVVVPGSGFRQVPGTWHFRCTILPQEDKIPAIVDRLTAFHQSFMDEFRD 543

BLAST of Sgr025362 vs. TAIR 10
Match: AT1G72330.3 (alanine aminotransferase 2 )

HSP 1 Score: 790.0 bits (2039), Expect = 2.9e-228
Identity = 387/482 (80.29%), Postives = 429/482 (89.00%), Query Frame = 0

Query: 756  VLKCEYAVRGEIVALAQKLQEELLTKPGSRSFDEILYCNIGNPQSLGQQPITFFREVLAL 815
            VLKCEYAVRGEIV +AQKLQE+L T   +  FDEI+YCNIGNPQSLGQ PI FFREVLAL
Sbjct: 72   VLKCEYAVRGEIVNIAQKLQEDLKTNKDAYPFDEIIYCNIGNPQSLGQLPIKFFREVLAL 131

Query: 816  CDHPSILNRNEIQGLFSEDAINQALQILKQIPGRATGAYSHSQGIKGLRDAIAAGIEARD 875
            CDH S+L+ +E  GLFS D+I++A +IL  IPGRATGAYSHSQGIKGLRD IAAGIEARD
Sbjct: 132  CDHASLLDESETHGLFSTDSIDRAWRILDHIPGRATGAYSHSQGIKGLRDVIAAGIEARD 191

Query: 876  GFPADPADLFLTDGASPA-------------VHMMMQLLISSEKDGILCPIPQYPLYSAS 935
            GFPADP D+FLTDGASPA             VHMMMQLL+SSEKDGIL PIPQYPLYSAS
Sbjct: 192  GFPADPNDIFLTDGASPAVTIKTKTLSHRVRVHMMMQLLLSSEKDGILSPIPQYPLYSAS 251

Query: 936  IALHGGTLVPYYLDEASGWGLEISELKKELESAKSKGISVRALVVINPGNPTGQVLTKEN 995
            IALHGG+LVPYYLDEA+GWGLEIS+LKK+LE A+SKGISVRALVVINPGNPTGQVL +EN
Sbjct: 252  IALHGGSLVPYYLDEATGWGLEISDLKKQLEEARSKGISVRALVVINPGNPTGQVLAEEN 311

Query: 996  QEQIVEFCKQEGLVLLADEVYQENIYVRDKKFHSFKKISRSMGYGENDISLVSFQSVSKG 1055
            Q  IV FCKQEGLVLLADEVYQEN+YV DKKFHSFKK++RS+GYGE DISLVSFQSVSKG
Sbjct: 312  QRDIVNFCKQEGLVLLADEVYQENVYVPDKKFHSFKKVARSLGYGEKDISLVSFQSVSKG 371

Query: 1056 YYGECGKRGGYMEVTGFSADVREQIYKVASVNLCSNISGQILASLVMNPPKVGDRIYESY 1115
            YYGECGKRGGYMEVTGF++DVREQIYK+ASVNLCSNISGQILASLVM+PPK GD  Y+SY
Sbjct: 372  YYGECGKRGGYMEVTGFTSDVREQIYKMASVNLCSNISGQILASLVMSPPKPGDDSYDSY 431

Query: 1116 CAERDGILSSLARRAKTLEAAFNSLENVTCNKAEGAMYLFPCIKLPEKAIKAAEAANTAP 1175
             AERDGILSS+A+RAKTLE A NSLE VTCN+AEGAMYLFP I LP+KAI+AAEA  TAP
Sbjct: 432  MAERDGILSSMAKRAKTLEDALNSLEGVTCNRAEGAMYLFPRINLPQKAIEAAEAEKTAP 491

Query: 1176 DAFYCRHLLNATGIVVVPGSGFGQVPGTWHFRCTILPQEDKIPAVVSRLTDFHRAFMYEY 1225
            DAFYC+ LLNATG+VVVPGSGFGQVPGTWHFRCTILPQEDKIPA+V+RLT+FH++FM E+
Sbjct: 492  DAFYCKRLLNATGVVVVPGSGFGQVPGTWHFRCTILPQEDKIPAIVNRLTEFHKSFMDEF 551

BLAST of Sgr025362 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 766.1 bits (1977), Expect = 4.5e-221
Identity = 396/570 (69.47%), Postives = 459/570 (80.53%), Query Frame = 0

Query: 19  KKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDRS---EAHAILKSSCSSTLYPDLCF 78
           KK+KK +LL+ +  LL V A+ GI AG +K+ N  R+    +HA+L+SSCSST YP+LC 
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKA-NEKRTLSPSSHAVLRSSCSSTRYPELCI 74

Query: 79  STISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKL-AALKNITHRERLALHDCL 138
           S +    G   ++++QKDVIE S+N+T+ AVE +YF V+KL    K +T RE+ ALHDCL
Sbjct: 75  SAVVTAGGV--ELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCL 134

Query: 139 ETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITNQETCLDGFSHDEADKSL 198
           ETIDETLDELH+ + DLH YP KK+L +HA DLKTL+SSAITNQETCLDGFSHD+ADK +
Sbjct: 135 ETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQV 194

Query: 199 RKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYES----TMGSRKLMEE----------- 258
           RK L +GQ  VEHMCSNALAM+KNMTDTDIAN+E     T  +RKL EE           
Sbjct: 195 RKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIA 254

Query: 259 -ADNDDDIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYI 318
            A   D   WP WLSAGDRRLLQ S V  D  VAADGSG F+TV+AAVAAAP  S KRY+
Sbjct: 255 GAGELDSEGWPTWLSAGDRRLLQGSGVKADATVAADGSGTFKTVAAAVAAAPENSNKRYV 314

Query: 319 IRIKAGVYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLA 378
           I IKAGVYRENVEV KKK NIMF+GDGR+ TIITGSRNVVDGSTTF+SATVAAVGE FLA
Sbjct: 315 IHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFLA 374

Query: 379 RDITFQNTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDF 438
           RDITFQNTAGPSKHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF++ CLI+GTVDF
Sbjct: 375 RDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVDF 434

Query: 439 IFGNAAAIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKN 498
           IFGNAA + Q+CDIHARRPNSGQKNMVTAQGR+DPNQNTGIVIQK RIGATSDL+ V+ +
Sbjct: 435 IFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKGS 494

Query: 499 FPTFLGRPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTSGR 558
           FPT+LGRPWKEYS+TVIMQS+ISDVI P+GW EW+G FALNTL Y EY NTG GA T+ R
Sbjct: 495 FPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTANR 554

Query: 559 VTWKGFRVIRSATEAEAFTAGRFIGGGGWL 569
           V W+GF+VI +A EA+ +TAG+FIGGGGWL
Sbjct: 555 VKWRGFKVITAAAEAQKYTAGQFIGGGGWL 581

BLAST of Sgr025362 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 760.8 bits (1963), Expect = 1.9e-219
Identity = 392/564 (69.50%), Postives = 457/564 (81.03%), Query Frame = 0

Query: 19  KKSKKKLLLALSATLLLVGAIVGIVAGVTKSRNSDR-----SEAHAILKSSCSSTLYPDL 78
           K +KK +L + +  LLL+ +IVGI A  T    + +     S +HAILKS CSSTLYP+L
Sbjct: 15  KNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPEL 74

Query: 79  CFSTISADDGAMKKVSNQKDVIELSINITVKAVEASYFRVQKL-AALKNITHRERLALHD 138
           CFS ++A  G  K++++QK+VIE S+N+T KAV+ +YF V+KL A  K +T RE  ALHD
Sbjct: 75  CFSAVAATGG--KELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHD 134

Query: 139 CLETIDETLDELHKAIADLHEYPNKKSLTQHADDLKTLLSSAITNQETCLDGFSHDEADK 198
           CLETIDETLDELH A+ DLH+YP +KSL +HADDLKTL+SSAITNQ TCLDGFS+D+AD+
Sbjct: 135 CLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADR 194

Query: 199 SLRKKLEEGQKEVEHMCSNALAMVKNMTDTDIANYE--------STMGSRKLMEEADNDD 258
            +RK L +GQ  VEHMCSNALAM+KNMT+TDIAN+E        +   +RKL E   + D
Sbjct: 195 KVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLD 254

Query: 259 DIKWPEWLSAGDRRLLQSSSVTPDVVVAADGSGNFRTVSAAVAAAPVKSKKRYIIRIKAG 318
              WP+WLS GDRRLLQ S++  D  VA DGSG+F TV+AAVAAAP KS KR++I IKAG
Sbjct: 255 SDGWPKWLSVGDRRLLQGSTIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAG 314

Query: 319 VYRENVEVPKKKTNIMFVGDGRSNTIITGSRNVVDGSTTFNSATVAAVGEGFLARDITFQ 378
           VYRENVEV KKKTNIMF+GDGR  TIITGSRNVVDGSTTF+SATVAAVGE FLARDITFQ
Sbjct: 315 VYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQ 374

Query: 379 NTAGPSKHQAVALRVGADLSAFYQCDMLAYQDTLYVHSNRQFYINCLISGTVDFIFGNAA 438
           NTAGPSKHQAVALRVG+D SAFYQCDM AYQDTLYVHSNRQF++ C I+GTVDFIFGNAA
Sbjct: 375 NTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAA 434

Query: 439 AIFQNCDIHARRPNSGQKNMVTAQGRSDPNQNTGIVIQKSRIGATSDLRPVQKNFPTFLG 498
           A+ Q+CDI+ARRPNSGQKNMVTAQGRSDPNQNTGIVIQ  RIG TSDL  V+  FPT+LG
Sbjct: 435 AVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLG 494

Query: 499 RPWKEYSRTVIMQSSISDVIDPKGWHEWSGNFALNTLFYGEYQNTGPGASTSGRVTWKGF 558
           RPWKEYSRTVIMQS ISDVI P+GWHEWSG+FAL+TL Y EY N G GA T+ RV WKG+
Sbjct: 495 RPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGY 554

Query: 559 RVIRSATEAEAFTAGRFIGGGGWL 569
           +VI S TEA+ FTAG+FIGGGGWL
Sbjct: 555 KVITSDTEAQPFTAGQFIGGGGWL 576