Sgr025296 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025296 vs. NCBI nr
Match: CAN78640.1 (hypothetical protein VITISV_031739 [Vitis vinifera])

HSP 1 Score: 95.9 bits (237), Expect = 5.9e-16
Identity = 86/191 (45.03%), Postives = 108/191 (56.54%), Query Frame = 0

Query: 42  RRQRILHSPNINVFIQTLPVIITPKLPHERLRLILLKKLMLLLLLLHSAVPIQRIGIQKH 101
           +R  I+H P I++ +  L       L H  +R+  +KK+MLL++++ +    QRI I   
Sbjct: 763 QRIDIMHDPCIDILVLLL-------LHHVIMRM-GMKKMMLLMMVIRT----QRIRI--- 822

Query: 102 DLGGNGVVALSRRRRSSSGGGGLLFTNGELVEDDVELGLAVVDLALEVADDAVAAADGVE 161
              GNG             GG      G+++ED VEL L V+DLALEV DD VA ADGV 
Sbjct: 823 --NGNGRWGFRYAGLLLLVGGA---AYGQVMEDKVELRLGVLDLALEVTDDTVATADGVG 882

Query: 162 GADVGLEDDGAHGLFLLIGVEILDDL-------------ELALAIVGEIRGENAVGGALS 220
           GADV LE+DGAHGL L+  VE+ DDL             ELALA V +IRGENAV GA S
Sbjct: 883 GADVRLENDGAHGLVLVRRVEVADDLGDVADPKQFMAVEELALATVRKIRGENAVRGAFS 933

BLAST of Sgr025296 vs. NCBI nr
Match: PON48152.1 (hypothetical protein PanWU01x14_239400 [Parasponia andersonii])

HSP 1 Score: 89.0 bits (219), Expect = 7.2e-14
Identity = 76/159 (47.80%), Postives = 99/159 (62.26%), Query Frame = 0

Query: 77  LKKLMLLLLL---LHSAVPIQRIGIQKHDLGGNGVVALSRRRRSSSGGGGLLFTNGELVE 136
           +K+L++L+++   +H+ +      I+   +GG      + R RSSS  G    T  E++E
Sbjct: 1   MKELIMLMMMKPRMHAII------IEMIIMGGG-----TLRWRSSSCCGA---TKLEMLE 60

Query: 137 DDVELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLI-GVEILDDL----- 196
           ++VELGL  V+LALEV DDAVAAA+GV GA VGLEDDGAHG+ L   GVE+ DDL     
Sbjct: 61  NEVELGLGEVNLALEVLDDAVAAANGVGGAGVGLEDDGAHGVVLGDGGVELPDDLGDVAD 120

Query: 197 --------ELALAIVGEIRGENAVGGALSALVFAGGAGL 219
                   EL L +VGE+RGENAV GAL ALV A  A L
Sbjct: 121 AEELVRVEELPLRVVGEVRGENAVRGALPALVLACSASL 145

BLAST of Sgr025296 vs. NCBI nr
Match: RWW56882.1 (hypothetical protein BHE74_00036373 [Ensete ventricosum])

HSP 1 Score: 88.6 bits (218), Expect = 9.4e-14
Identity = 74/168 (44.05%), Postives = 94/168 (55.95%), Query Frame = 0

Query: 120 GGGGLLFTNGELVEDDVELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLI 179
           G G L   +GEL + + EL    +DL LEVADDAVA ADG+E   +GL  DG HG  +L+
Sbjct: 106 GLGSLRTADGELPQHEGELIGVALDLVLEVADDAVATADGIESTHIGLVYDGFHGFGMLV 165

Query: 180 GVEILDDL-------------ELALAIVGEIRGENAVGGALSALVFAGGA-----GLGGG 239
           G+E+ DD              EL L +  ++RGE AVG AL+ALVFAGGA     GLGGG
Sbjct: 166 GIEVADDFEDVADAEQAVGVEELVLLVGRKVRGEAAVGVALAALVFAGGAGLGCGGLGGG 225

Query: 240 GGGGGGGVGSWKMVVVAAAHNFNLFRILWGGAVGVGGYGDIGCGCGSG 270
           GGGGGGG  +   V+ A+    +           + G+GD G G G G
Sbjct: 226 GGGGGGGRKAGGGVLAASCFGGSCMGCGASDHYKIEGWGDEG-GTGPG 272

BLAST of Sgr025296 vs. NCBI nr
Match: RRT76571.1 (hypothetical protein B296_00016981 [Ensete ventricosum])

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-13
Identity = 81/205 (39.51%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 80  LMLLLLLLHSAVPIQRIGIQKHDLGGNGV---VALSRRRRSSSGGGGLLFTNGELVEDDV 139
           ++LL+LL++    I  I    H LG   V   + +  +     G G L   +GEL + + 
Sbjct: 15  VLLLILLVNRRENITTINHVIHILGRGRVKQKIRVDVQVGRDRGLGSLRTADGELPQHEG 74

Query: 140 ELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLIGVEILDDL--------- 199
           EL    +DL LEVADDAVA ADG+E   +GL  DG HG  +L+G+E+ DD          
Sbjct: 75  ELIGVALDLVLEVADDAVATADGIESTHIGLVYDGFHGFGMLVGIEVADDFEDIADAEQA 134

Query: 200 ----ELALAIVGEIRGENAVGGALSALVFAGGAGLGGGGGGGGGGVGSWKMVVVAAAHNF 259
               EL L +  ++RGE AVG AL+ALVFAGGAGLG GG GGGGG G             
Sbjct: 135 VGVEELVLLVGRKVRGEAAVGVALAALVFAGGAGLGCGGLGGGGGGGGGG---------- 194

Query: 260 NLFRILWGGAVGVGGYGDIGCGCGS 269
              R   GG +    +G    GCG+
Sbjct: 195 ---RKAGGGVLAASCFGGSCMGCGA 206

BLAST of Sgr025296 vs. NCBI nr
Match: RWV86912.1 (hypothetical protein GW17_00051143, partial [Ensete ventricosum])

HSP 1 Score: 88.2 bits (217), Expect = 1.2e-13
Identity = 81/205 (39.51%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 80  LMLLLLLLHSAVPIQRIGIQKHDLGGNGV---VALSRRRRSSSGGGGLLFTNGELVEDDV 139
           ++LL+LL++    I  I    H LG   V   + +  +     G G L   +GEL + + 
Sbjct: 3   VLLLILLVNRRENITTINHVIHILGRGRVKQKIRVDVQVGRDRGLGSLRTADGELPQHEG 62

Query: 140 ELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLIGVEILDDL--------- 199
           EL    +DL LEVADDAVA ADG+E   +GL  DG HG  +L+G+E+ DD          
Sbjct: 63  ELIGVALDLVLEVADDAVATADGIESTHIGLVYDGFHGFGMLVGIEVADDFEDVADAEQA 122

Query: 200 ----ELALAIVGEIRGENAVGGALSALVFAGGAGLGGGGGGGGGGVGSWKMVVVAAAHNF 259
               EL L +  ++RGE AVG AL+ALVFAGGAGLG GG GGGGG G             
Sbjct: 123 VGVEELVLLVGRKVRGEAAVGVALAALVFAGGAGLGCGGLGGGGGGGGGG---------- 182

Query: 260 NLFRILWGGAVGVGGYGDIGCGCGS 269
              R   GG +    +G    GCG+
Sbjct: 183 ---RKAGGGVLAASCFGGSCMGCGA 194

BLAST of Sgr025296 vs. ExPASy TrEMBL
Match: A5B4B4 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=VITISV_031739 PE=4 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 2.9e-16
Identity = 86/191 (45.03%), Postives = 108/191 (56.54%), Query Frame = 0

Query: 42  RRQRILHSPNINVFIQTLPVIITPKLPHERLRLILLKKLMLLLLLLHSAVPIQRIGIQKH 101
           +R  I+H P I++ +  L       L H  +R+  +KK+MLL++++ +    QRI I   
Sbjct: 763 QRIDIMHDPCIDILVLLL-------LHHVIMRM-GMKKMMLLMMVIRT----QRIRI--- 822

Query: 102 DLGGNGVVALSRRRRSSSGGGGLLFTNGELVEDDVELGLAVVDLALEVADDAVAAADGVE 161
              GNG             GG      G+++ED VEL L V+DLALEV DD VA ADGV 
Sbjct: 823 --NGNGRWGFRYAGLLLLVGGA---AYGQVMEDKVELRLGVLDLALEVTDDTVATADGVG 882

Query: 162 GADVGLEDDGAHGLFLLIGVEILDDL-------------ELALAIVGEIRGENAVGGALS 220
           GADV LE+DGAHGL L+  VE+ DDL             ELALA V +IRGENAV GA S
Sbjct: 883 GADVRLENDGAHGLVLVRRVEVADDLGDVADPKQFMAVEELALATVRKIRGENAVRGAFS 933

BLAST of Sgr025296 vs. ExPASy TrEMBL
Match: A0A2P5BH77 (Uncharacterized protein OS=Parasponia andersonii OX=3476 GN=PanWU01x14_239400 PE=4 SV=1)

HSP 1 Score: 89.0 bits (219), Expect = 3.5e-14
Identity = 76/159 (47.80%), Postives = 99/159 (62.26%), Query Frame = 0

Query: 77  LKKLMLLLLL---LHSAVPIQRIGIQKHDLGGNGVVALSRRRRSSSGGGGLLFTNGELVE 136
           +K+L++L+++   +H+ +      I+   +GG      + R RSSS  G    T  E++E
Sbjct: 1   MKELIMLMMMKPRMHAII------IEMIIMGGG-----TLRWRSSSCCGA---TKLEMLE 60

Query: 137 DDVELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLI-GVEILDDL----- 196
           ++VELGL  V+LALEV DDAVAAA+GV GA VGLEDDGAHG+ L   GVE+ DDL     
Sbjct: 61  NEVELGLGEVNLALEVLDDAVAAANGVGGAGVGLEDDGAHGVVLGDGGVELPDDLGDVAD 120

Query: 197 --------ELALAIVGEIRGENAVGGALSALVFAGGAGL 219
                   EL L +VGE+RGENAV GAL ALV A  A L
Sbjct: 121 AEELVRVEELPLRVVGEVRGENAVRGALPALVLACSASL 145

BLAST of Sgr025296 vs. ExPASy TrEMBL
Match: A0A427AK94 (Uncharacterized protein OS=Ensete ventricosum OX=4639 GN=B296_00016981 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-14
Identity = 81/205 (39.51%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 80  LMLLLLLLHSAVPIQRIGIQKHDLGGNGV---VALSRRRRSSSGGGGLLFTNGELVEDDV 139
           ++LL+LL++    I  I    H LG   V   + +  +     G G L   +GEL + + 
Sbjct: 15  VLLLILLVNRRENITTINHVIHILGRGRVKQKIRVDVQVGRDRGLGSLRTADGELPQHEG 74

Query: 140 ELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLIGVEILDDL--------- 199
           EL    +DL LEVADDAVA ADG+E   +GL  DG HG  +L+G+E+ DD          
Sbjct: 75  ELIGVALDLVLEVADDAVATADGIESTHIGLVYDGFHGFGMLVGIEVADDFEDIADAEQA 134

Query: 200 ----ELALAIVGEIRGENAVGGALSALVFAGGAGLGGGGGGGGGGVGSWKMVVVAAAHNF 259
               EL L +  ++RGE AVG AL+ALVFAGGAGLG GG GGGGG G             
Sbjct: 135 VGVEELVLLVGRKVRGEAAVGVALAALVFAGGAGLGCGGLGGGGGGGGGG---------- 194

Query: 260 NLFRILWGGAVGVGGYGDIGCGCGS 269
              R   GG +    +G    GCG+
Sbjct: 195 ---RKAGGGVLAASCFGGSCMGCGA 206

BLAST of Sgr025296 vs. ExPASy TrEMBL
Match: A0A444CM14 (Uncharacterized protein (Fragment) OS=Ensete ventricosum OX=4639 GN=GW17_00051143 PE=4 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 5.9e-14
Identity = 81/205 (39.51%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 80  LMLLLLLLHSAVPIQRIGIQKHDLGGNGV---VALSRRRRSSSGGGGLLFTNGELVEDDV 139
           ++LL+LL++    I  I    H LG   V   + +  +     G G L   +GEL + + 
Sbjct: 3   VLLLILLVNRRENITTINHVIHILGRGRVKQKIRVDVQVGRDRGLGSLRTADGELPQHEG 62

Query: 140 ELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLIGVEILDDL--------- 199
           EL    +DL LEVADDAVA ADG+E   +GL  DG HG  +L+G+E+ DD          
Sbjct: 63  ELIGVALDLVLEVADDAVATADGIESTHIGLVYDGFHGFGMLVGIEVADDFEDVADAEQA 122

Query: 200 ----ELALAIVGEIRGENAVGGALSALVFAGGAGLGGGGGGGGGGVGSWKMVVVAAAHNF 259
               EL L +  ++RGE AVG AL+ALVFAGGAGLG GG GGGGG G             
Sbjct: 123 VGVEELVLLVGRKVRGEAAVGVALAALVFAGGAGLGCGGLGGGGGGGGGG---------- 182

Query: 260 NLFRILWGGAVGVGGYGDIGCGCGS 269
              R   GG +    +G    GCG+
Sbjct: 183 ---RKAGGGVLAASCFGGSCMGCGA 194

BLAST of Sgr025296 vs. ExPASy TrEMBL
Match: E5GCB2 (Chaperonin-60 kDa protein OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 6.6e-13
Identity = 79/223 (35.43%), Postives = 120/223 (53.81%), Query Frame = 0

Query: 18  MIVSVELDDIKIINRSLLDMQRRRRRQRILHSPNINVFI-QTLPVIITPKLPHERLRLIL 77
           M+ +  +D +K++  +L+D  R R   RIL+SPN++  I QTL ++I P   H+    I 
Sbjct: 793 MVKAGIVDPLKVVRTALVDASRTR---RILNSPNLHFLIFQTLSILIFPN--HKPFPFIS 852

Query: 78  LKKLMLLLLLLHSAVPIQRIGIQKHDLGGNGVVALSRRRRSSSGGGGLLFTNGELVEDDV 137
             KLML   +    +P Q  G                   SS G  GLL TN +L+E ++
Sbjct: 853 FHKLMLNPAISTHRIPFQNRGSSS----------------SSRGSSGLLLTNRQLMEHNL 912

Query: 138 ELGLAVVDLALEVADDAVAAADGVEGADVGLEDDGAHGLFLLIGVEILDDLELALAIVGE 197
           +L L V +L L+++++ +A A+ + G+++ L+++                 EL L IV E
Sbjct: 913 KLRLIVFNLTLKISNNPIATANRINGSNIRLKNN-----------------ELTLTIVRE 972

Query: 198 IRGENAVGGALSALVFAGGAGLGGGGGGG--GGGVGSWKMVVV 238
           IR ENA+G AL++LVF    GLGGGG GG  GGG G  ++VVV
Sbjct: 973 IRSENAIGSALTSLVFTSSTGLGGGGSGGCDGGGGGFGEVVVV 977