Sgr025295 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025295
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionChaperonin CPN60-like 2
Locationtig00004836: 1286370 .. 1291758 (+)
RNA-Seq ExpressionSgr025295
SyntenySgr025295
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTATCGTGTAGCTTCCAAATTAGCTTCCTCCATCGGTTCCTCTACGTCGAGAAAACTTGTAAGAAGAAATAAGATCTACTACTCGTCGCATTTCTCTGCGTTCGGCTCTCCCTCCCTCTTTTCCTTCCTAATATTTGGTTGCTGAAGTTGTTGCACTGAGGATGAAACTGGAGACGGAATTGAATTTTGTTTACAATTGCAGTATGTTAGTTTAGATTATGAATCTTGTTTTCTCTGACTAGCCAAGTTTCGGGAACTTTGATTTTTCCCTCCTCTTTTTAAGTTTTAGAATCATTCTTTGATTTGCACGCGCAATTCGTTTAAGGAAGTAATGAACATGGTTTATGGTATTTTTTACTGTTGAATGCTGTATTAAACAAGAGATATGGTTATTAAAGTTGATTGGTTTGGTTTGTCCAATCAAAACAGCTGCGGGTTTTGATTCTTGTGTTTTGCGATTGTCCTTGGTTTTGATTTTGATGAGTACGAACTGATATTGATTGATATTTTAAAAATTAAAGTTGGTTTTCTCTGGATTTAAAGGTATTTAGCAGGGTTACAAGCAGCAGAAATTATGCGGCAAAGGATATCAAATTTGGGGTTGGAGCTCGTGCAGCTATGCTCCAAGGTGTCTCGGAGGTTGCTGAAGCTGTTAAAGTGACAATGGGACCAAAGGTATAGGACCTGATTGGTCATTTGACATTGGTGGTTTTTCTGCTACTTCTCTTGCTGGGAACGTCAAAATTTGTAAGGGTGGAAAGAACTGTCTGCAGTTTTCTTTAAGATGTTTACACATTCCTCGACGGATATTTGAACTTACTAGGGGACCTCAACTGATTAATAATTATTGCCTCTTTCTCATTGTGTTCAATGAAATTAGGGTCGCAATGTAATTATTGATAAAAGTCACGGCAGCCCAAAAGTCACAAAGGATGGTGTTACTGTAGCCAATAGTATCAAATTCAAGGATAAGGCTAAAAATGTTGGGGCAGATCTCGTAAAGCAAGTTGCGAGTGCCACAAACACTGCTGCTGGAGACGGTAGCTTCTTCCTGTAACCCAAATTGCTTCTTCTTAATTTTTTTTAATAATAAATACTTATTTATTACGAATATCATTCTATGTCGAGAAGTTCAAGCTATATTGAAATTGGGACTAACAACACTAGAATTTGTGGTGGAAGGTATGAGACATAAAGTAACTAAAGATTGGCATTGGATGATAATTAGTATTTTTGCATTTGAAATCAAGCAAATAGGGAAGTATCATTGCGCTTTCTTCAATAAAGAATTTAAATTGAACTTATGGTCATGGATGGGGCTTCTGCAGTTACAGTTTAATCTTTCACTACCTGATTCTGTGTGTGCCCCTGATATGCTCGTTATTTGTAGAAATCGAAGTTCATCCACTGGTACAGTTGGTGGAACAGATAGCAGGACATGTTTGCATGCCATTTTAATGGTGTTTGAACATGGGTTGTGTTGTTTGTTGGCTTACAAAGATTTAGCACTTTAGAATCCAAGCTCTGTAAAGCATGAAGTATTTAGAGAACTTTTTTTGAACAAAAAACTTGGATAAGCCAATTGTTTGTTCATGAATGAATTGCTTGTTTTGTTGCAGGTACAACTTGTGCAACTGTTCTGACTCAGGCCATACTCACCGAAGGTTGCAAGTCAATAGCTGCAGGTGTAAGTGTGATGGATTTGCGCATAGGTATCAAAACAGCAGTTGATGCTGTTATCTCTGAGTTGAAAAGCAGAGCATTGATGATAAGCACCCCAGAAGAAATTACTCAGGTAATAGGGTTCCAAAGGGAACCTGTTTTTTTATTTTTCAAGTGGGATTCTTTAGATGGGACAAAGTTTGTATTTGGATTTTGTAGTCGGTAATAATTGATTCTAAGTTGGAAAAGTGCATTTTACATTTTCTTTTCGTACAGATGACATCTGTGATATTGCTTTTGGATGAAGGGCCTCCTTGTTGATACTCTTTCTTGAGTTTTTAATTGTCGTGAGTGTGTTAATCTTAGAGCAGTTGCAGAACAATTTCATTTTCTGTAATAATAGTTTTCTTCTATGGATATACACAGAATGTTCTTTCCTCAACTATGTTTACTTTTCTGTATATTATTCGTTATTCGTTATTCGTCATTTTTATTCTAGCTTTGCCACTATTTCTTCTACTTTTATTACCTTTTTATTTTTGTTATTATGATAAACAGGTTGCCACTATTTCTGCAAATGGTGAACGTGAGATTGGAGAATTGATAGCGAGGGCAATGGAGAAAGTTGGAAGGGAAGGAGTGATTACTGTTTCTGTATGCATTCTTTCCCCACTTGCTTTTATATAATATGGTAATTGAACTTATGAGAATTTCAAATTTGTACTCTAGTAATTTCTCTGCTTCTGCAGGATGGAAATACTCTGGAAGATGAATTGGAAGTGGTGGAAGGAATGAAGCTAGGCAGAGGTTTCATTTCTCCTTATTTTATTAACGATCAAAAGACCCAGAAATGTGTAAGTTAAGATTTCTTTTGCATTAGTTAAAACCTTTAGGTGCTTAATGCACTAATAATATGATTGCGGATGTCAAGTATGAGAAAATATCATAAACTGCCTTGTGGCATTTGTGCTCCTTAGTCAGACTTTGTATTTCCGTGTAGGTCAAGTGAAAATTATGTGAAGTGTGTCGCATGTAATTTTAATATGGAATCTATAATTTATCAAAATGCGCAGGAACTAGAAAACCCTCTCATCCTCATACATGAAAAGAAGATTTCAGATATGAATTTACTCCTGAGAGTACTAGAACTTGCAGTAGAGGTATCAATCTTGATTTTGTTTTTTAAATGTGCAATGTTGTGTGAATAGTGTGCCTGAACCTTACAAACTAACACGATAAATTCATTCTCTGAGCAGAAGAAAAGGGCACTTCTTGTTGTAGCCGAGGATGTTGAGAGTGATGCACTTGCCATGCTAATACTTAACAAGCATCATACTGGCCTGAAGGTGATTGCTTGTTTATTTATTTATTTTAAGAAATATGGCTTATTTTAATTTTCTCTTATCTTTATTGAATGTTTTTGCATGCCGGTGTAAGTTATATAAAAGATCAAGTGTCTCTCCAATAGAACTGAGTAATTAAAGTAGACACAGGCATCATTGCAGTCACTTTTAAGAAGCTATGAATCTCTTTGATTAGCAGAGATTTATTCTTATGTAATTGCGAATATTCTTATGGCCATCAAATCTCTTTTAAAACTATCTTCAAAACTCTTCCAAATCTTCTCCATTCCAATGCTTGTAGCATCCATGTTGCTTGTTACATATATTTTTATCTCAAATGGCTTATAACATGAAATACGGGAAAATATTTTAGTCGGCTTTCCAGCATGCTTTTGTCAGTTGCAAAATGTCACTAATGACTTGCTTTCTTGAGTGAGTTTTGAAAAAGTTCTTGGTGAATGAAAATGAAACAATGTATTCTGAAAAGTTTAAACCCCCATAAACGTTTTTTCTCCTTTCAGGTTTGTGCCATCAAAGCTCCTGGTTTTGGGGATAACAGAAGAGCAAATTTGGATGATCTTGCCATTTTGACAGGAGGAGAGGTGATACAAAGTGCTCTAATCCAAGTTCATTGTATCAACTTTTTGTAAGGTAGTTTAGTTATATTTAGAATGTGCGACAGGTCATCACTGATGAACGTGGTTTAACTCTTGACAAAGTGCAAATAGATATGCTTGGTACTGCAAAAAAGGTTCACGCCCAGTCCATTATCGCACTTCTTTCTTTTTACTGTTGTTTTATGTTTAAATCTTCATGCACAATGTAACTTCTTCTTAGGTTACTGTCTCTCTGACGACACAATCGTTCTTCATGGAGGCGGTGATAAGAAACTAATTGAAGAAAGATGTGAGCAGGTTTGTTATGGCTAACTAAGTTGTTCCTGACGGGATTCTGTTGAATCAGCTTTCTAAAATTGTTCTCTGGAGGCTGGAAAGGAGATTATAGCTTAATTTAAGTTGGAGTCGTCTTAGAGACCATGGGAAAATGTAATAAAGAGCAGCATCTGGTTCATGAATTCCTACTAATGCTGTAGAACGATTGCCTTGCAGCTGAGAACAACTATTGACAGGAGTACTGCAATGTTTGACAAGGAAAAAGCTCAGGAAAGATTATCAAAACTCTCTGGTGGTGTAGCAGTCTTCAAGGTATAATGTTTGCCTTAATAATGATTTGAAAGAATTTTGTATGCCCCATTTGTAAAAAATTCGGTTTTCTTGCAGGTAGGTGGGGTAAGCGAGGCAGAAGTTGGGGAGAGGAAAGACAGAGTCACAGATGCTTTGAATGCCACAAGAGCAGCTGTAGAAGAAGGAATTGTGCCAGGTAAGCTTTTCTTTTACTGGAAAAAGTATTGTTGGAACTTGAAAGCTTATCTGTGCTGTTGGTGGATAATTGCTATCATTAGGTGGTGGAGTTGCCCTTTTGCATGCAACGAAGGTTCTGGAGGAACTTCAAGCTCAAAATGAAGACCAGAAAAGAGGAATAGAAATAGTGCAACATGCTCTTAGGGTTTGTTTGTTTCTTTGAGTTCTCAGCACTACTTTTTGGGGCTTATCCACAGCTTGTTTTCTTAAATCAAAGCTTCGGTTTTCTACAGGCACCTATGTTTACAATAGTTTCAAATGCTGGATATGATGGTGCTTTGGTTCTTGGAAAATTATTGGAACAGGATGATCGTAATTTGGGTTTTGACGCTGCCAAAGGTGGGTTGCTTGCAGCAGTTGGAAAATTTTTAAGTTCATTAATCCCTAATATGTTACAAGATATCCATTTTCCACTTAACGTAGAAGGATTTATTAACCTTTGCTCTCATGAAATCTGCCCCGGATCATAAAATTTGATTGTCAATCTAGATTCTCTCTTTTCCTTTCCTGCTTGCGTCTTCAGTTGAATTCCCGTGTTTTTTTTTTTAAAATTAATTAGAACTAATTGTTTTTCTAATCTCTCAATCCATGTCAATTCTTGTGCAAAACAGGTGCATATGTTGACATGGTGAAGGCTGGAATTGTAGATCCTCTGAAAGTTGTGAGAACCGCTTTAGTTGATGCTGCCAGGTGAATGTTCTACTTCTACCCCATGTCCTTTGTCAAAAAGTTGACAAACGTAGTTTATTTCGGTCTCTATGCAATCCTCTAAAGCCTTGCAACAGAAATTAGACTACAAAACGTTATCCACTTCCAAGCACACTAATTAAATGTTTGATGCTCTTGAATTTCCAGCGTCTCTTTGCTGTTGACAACAGCCGAGGCAGCCATAGTGGAAGATCCAAATGATAAGAACAAGCTTCCGAGTAGAATGCCAGCCATGGATGATATGGGCTACTAG

mRNA sequence

ATGTATCGTGTAGCTTCCAAATTAGCTTCCTCCATCGGTTCCTCTACGTCGAGAAAACTTGTATTTAGCAGGGTTACAAGCAGCAGAAATTATGCGGCAAAGGATATCAAATTTGGGGTTGGAGCTCGTGCAGCTATGCTCCAAGGTGTCTCGGAGGTTGCTGAAGCTGTTAAAGTGACAATGGGACCAAAGGGTCGCAATGTAATTATTGATAAAAGTCACGGCAGCCCAAAAGTCACAAAGGATGGTGTTACTGTAGCCAATAGTATCAAATTCAAGGATAAGGCTAAAAATGTTGGGGCAGATCTCGTAAAGCAAGTTGCGAGTGCCACAAACACTGCTGCTGGAGACGGTACAACTTGTGCAACTGTTCTGACTCAGGCCATACTCACCGAAGGTTGCAAGTCAATAGCTGCAGGTGTAAGTGTGATGGATTTGCGCATAGGTATCAAAACAGCAGTTGATGCTGTTATCTCTGAGTTGAAAAGCAGAGCATTGATGATAAGCACCCCAGAAGAAATTACTCAGGTTGCCACTATTTCTGCAAATGGTGAACGTGAGATTGGAGAATTGATAGCGAGGGCAATGGAGAAAGTTGGAAGGGAAGGAGTGATTACTGTTTCTGATGGAAATACTCTGGAAGATGAATTGGAAGTGGTGGAAGGAATGAAGCTAGGCAGAGGTTTCATTTCTCCTTATTTTATTAACGATCAAAAGACCCAGAAATGTGAACTAGAAAACCCTCTCATCCTCATACATGAAAAGAAGATTTCAGATATGAATTTACTCCTGAGAGTACTAGAACTTGCAGTAGAGAAGAAAAGGGCACTTCTTGTTGTAGCCGAGGATGTTGAGAGTGATGCACTTGCCATGCTAATACTTAACAAGCATCATACTGGCCTGAAGGTTTGTGCCATCAAAGCTCCTGGTTTTGGGGATAACAGAAGAGCAAATTTGGATGATCTTGCCATTTTGACAGGAGGAGAGGTCATCACTGATGAACGTGGTTTAACTCTTGACAAAGTGCAAATAGATATGCTTGGTTACTGTCTCTCTGACGACACAATCGTTCTTCATGGAGGCGGTGATAAGAAACTAATTGAAGAAAGATGTGAGCAGCTGAGAACAACTATTGACAGGAGTACTGCAATGTTTGACAAGGAAAAAGCTCAGGAAAGATTATCAAAACTCTCTGGTGGTGTAGCAGTCTTCAAGGTAGGTGGGGTAAGCGAGGCAGAAGTTGGGGAGAGGAAAGACAGAGTCACAGATGCTTTGAATGCCACAAGAGCAGCTGTAGAAGAAGGAATTGTGCCAGGTGGTGGAGTTGCCCTTTTGCATGCAACGAAGGTTCTGGAGGAACTTCAAGCTCAAAATGAAGACCAGAAAAGAGGAATAGAAATAGTGCAACATGCTCTTAGGGCACCTATGTTTACAATAGTTTCAAATGCTGGATATGATGGTGCTTTGGTTCTTGGAAAATTATTGGAACAGGATGATCGTAATTTGGGTTTTGACGCTGCCAAAGGTGCATATGTTGACATGGTGAAGGCTGGAATTGTAGATCCTCTGAAAGTTGTGAGAACCGCTTTAGTTGATGCTGCCAGCGTCTCTTTGCTGTTGACAACAGCCGAGGCAGCCATAGTGGAAGATCCAAATGATAAGAACAAGCTTCCGAGTAGAATGCCAGCCATGGATGATATGGGCTACTAG

Coding sequence (CDS)

ATGTATCGTGTAGCTTCCAAATTAGCTTCCTCCATCGGTTCCTCTACGTCGAGAAAACTTGTATTTAGCAGGGTTACAAGCAGCAGAAATTATGCGGCAAAGGATATCAAATTTGGGGTTGGAGCTCGTGCAGCTATGCTCCAAGGTGTCTCGGAGGTTGCTGAAGCTGTTAAAGTGACAATGGGACCAAAGGGTCGCAATGTAATTATTGATAAAAGTCACGGCAGCCCAAAAGTCACAAAGGATGGTGTTACTGTAGCCAATAGTATCAAATTCAAGGATAAGGCTAAAAATGTTGGGGCAGATCTCGTAAAGCAAGTTGCGAGTGCCACAAACACTGCTGCTGGAGACGGTACAACTTGTGCAACTGTTCTGACTCAGGCCATACTCACCGAAGGTTGCAAGTCAATAGCTGCAGGTGTAAGTGTGATGGATTTGCGCATAGGTATCAAAACAGCAGTTGATGCTGTTATCTCTGAGTTGAAAAGCAGAGCATTGATGATAAGCACCCCAGAAGAAATTACTCAGGTTGCCACTATTTCTGCAAATGGTGAACGTGAGATTGGAGAATTGATAGCGAGGGCAATGGAGAAAGTTGGAAGGGAAGGAGTGATTACTGTTTCTGATGGAAATACTCTGGAAGATGAATTGGAAGTGGTGGAAGGAATGAAGCTAGGCAGAGGTTTCATTTCTCCTTATTTTATTAACGATCAAAAGACCCAGAAATGTGAACTAGAAAACCCTCTCATCCTCATACATGAAAAGAAGATTTCAGATATGAATTTACTCCTGAGAGTACTAGAACTTGCAGTAGAGAAGAAAAGGGCACTTCTTGTTGTAGCCGAGGATGTTGAGAGTGATGCACTTGCCATGCTAATACTTAACAAGCATCATACTGGCCTGAAGGTTTGTGCCATCAAAGCTCCTGGTTTTGGGGATAACAGAAGAGCAAATTTGGATGATCTTGCCATTTTGACAGGAGGAGAGGTCATCACTGATGAACGTGGTTTAACTCTTGACAAAGTGCAAATAGATATGCTTGGTTACTGTCTCTCTGACGACACAATCGTTCTTCATGGAGGCGGTGATAAGAAACTAATTGAAGAAAGATGTGAGCAGCTGAGAACAACTATTGACAGGAGTACTGCAATGTTTGACAAGGAAAAAGCTCAGGAAAGATTATCAAAACTCTCTGGTGGTGTAGCAGTCTTCAAGGTAGGTGGGGTAAGCGAGGCAGAAGTTGGGGAGAGGAAAGACAGAGTCACAGATGCTTTGAATGCCACAAGAGCAGCTGTAGAAGAAGGAATTGTGCCAGGTGGTGGAGTTGCCCTTTTGCATGCAACGAAGGTTCTGGAGGAACTTCAAGCTCAAAATGAAGACCAGAAAAGAGGAATAGAAATAGTGCAACATGCTCTTAGGGCACCTATGTTTACAATAGTTTCAAATGCTGGATATGATGGTGCTTTGGTTCTTGGAAAATTATTGGAACAGGATGATCGTAATTTGGGTTTTGACGCTGCCAAAGGTGCATATGTTGACATGGTGAAGGCTGGAATTGTAGATCCTCTGAAAGTTGTGAGAACCGCTTTAGTTGATGCTGCCAGCGTCTCTTTGCTGTTGACAACAGCCGAGGCAGCCATAGTGGAAGATCCAAATGATAAGAACAAGCTTCCGAGTAGAATGCCAGCCATGGATGATATGGGCTACTAG

Protein sequence

MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVTMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTTCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKTQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTGLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLSDDTIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAPMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAASVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY
Homology
BLAST of Sgr025295 vs. NCBI nr
Match: XP_022992465.1 (LOW QUALITY PROTEIN: chaperonin CPN60-like 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 999.6 bits (2583), Expect = 1.1e-287
Identity = 535/574 (93.21%), Postives = 552/574 (96.17%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV SKLA    SSTSRKLV SRVTSSR+YAAKDI FG GARAAMLQGVSE+AEAVKVT
Sbjct: 275 MYRVVSKLA----SSTSRKLVCSRVTSSRSYAAKDINFGNGARAAMLQGVSEIAEAVKVT 334

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDG+TVA SI+FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 335 MGPKGRNVIIDKSHGSPKVTKDGITVAKSIQFKDKAKNVGADLVKQVASATNTAAGDGTT 394

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI
Sbjct: 395 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 454

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 455 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 514

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKC+LENPLILIHEKKISDMNL+LRVLELAVEKKR+LLVVAEDVESDALAMLILNKHH G
Sbjct: 515 QKCDLENPLILIHEKKISDMNLILRVLELAVEKKRSLLVVAEDVESDALAMLILNKHHAG 574

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERGLTLDKVQ++MLG         DDT
Sbjct: 575 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGLTLDKVQVEMLGTAKKVTVSLDDT 634

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 635 IILHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 694

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDA+NATRAAVEEGIVPGGGVALLHATKVL++LQAQN DQKRGIEIVQHALRAP
Sbjct: 695 ERKDRVTDAVNATRAAVEEGIVPGGGVALLHATKVLDDLQAQNADQKRGIEIVQHALRAP 754

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRNLG+DAAKG YVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 755 TFTIVSNAGYDGALVLGKLLEQDDRNLGYDAAKGVYVDMVKAGIVDPLKVVRTALVDAAS 814

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           +S+LLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY
Sbjct: 815 ISMLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 844

BLAST of Sgr025295 vs. NCBI nr
Match: KAG6575473.1 (Chaperonin CPN60-like 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 999.2 bits (2582), Expect = 1.4e-287
Identity = 535/574 (93.21%), Postives = 552/574 (96.17%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV SKLA    SSTSRKLV SRVTSSR+YAAKDI FG GARAAMLQGVSE+AEAVKVT
Sbjct: 367 MYRVVSKLA----SSTSRKLVCSRVTSSRSYAAKDINFGNGARAAMLQGVSEIAEAVKVT 426

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI+FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 427 MGPKGRNVIIDKSHGSPKVTKDGVTVAKSIQFKDKAKNVGADLVKQVASATNTAAGDGTT 486

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKS+ALMISTPEEITQVATI
Sbjct: 487 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSKALMISTPEEITQVATI 546

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 547 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 606

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKC+LENPLILIHEKKISDMNL+LRVLELAVEKKR+LLVVAEDVESDALAMLILNKHH G
Sbjct: 607 QKCDLENPLILIHEKKISDMNLILRVLELAVEKKRSLLVVAEDVESDALAMLILNKHHAG 666

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERGLTLDKVQ++MLG         DDT
Sbjct: 667 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGLTLDKVQVEMLGTAKKVTVSLDDT 726

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           ++LHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 727 LILHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 786

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVL++LQAQN DQKRGIEIVQHALRAP
Sbjct: 787 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLDDLQAQNADQKRGIEIVQHALRAP 846

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRNLG+DAAKG YVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 847 TFTIVSNAGYDGALVLGKLLEQDDRNLGYDAAKGVYVDMVKAGIVDPLKVVRTALVDAAS 906

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           +S+LLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY
Sbjct: 907 ISMLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 936

BLAST of Sgr025295 vs. NCBI nr
Match: XP_022149459.1 (chaperonin CPN60-like 2, mitochondrial isoform X2 [Momordica charantia])

HSP 1 Score: 997.7 bits (2578), Expect = 4.2e-287
Identity = 539/574 (93.90%), Postives = 548/574 (95.47%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRVASK A    SSTSRKLV SRVTSSRNYAAKDI FG GARAAML+GVSEVAEAVKVT
Sbjct: 1   MYRVASKFA----SSTSRKLVCSRVTSSRNYAAKDINFGDGARAAMLRGVSEVAEAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVAKSINFKDKAKNVGADLVKQVASATNTAAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI
Sbjct: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCELENPLILIHEKKISD+NLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHH G
Sbjct: 241 QKCELENPLILIHEKKISDLNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHAG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERGLTLDKVQ++MLG         DDT
Sbjct: 301 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGLTLDKVQVEMLGTAKKVTVSLDDT 360

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCEQLRTTID S AMFDK+KAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 361 IILHGGGDKKLIEERCEQLRTTIDNSAAMFDKDKAQERLSKLSGGVAVFKVGGVSEAEVG 420

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVL+ELQAQNEDQKRGIEIVQ ALRAP
Sbjct: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLDELQAQNEDQKRGIEIVQQALRAP 480

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRN G+DAAKGAYVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 481 TFTIVSNAGYDGALVLGKLLEQDDRNFGYDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           VSLLLTTAEAAIVEDPNDKN LPSRMPAMDDMGY
Sbjct: 541 VSLLLTTAEAAIVEDPNDKNTLPSRMPAMDDMGY 570

BLAST of Sgr025295 vs. NCBI nr
Match: XP_023548119.1 (chaperonin CPN60-like 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996.1 bits (2574), Expect = 1.2e-286
Identity = 533/574 (92.86%), Postives = 551/574 (95.99%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV S+LA    SSTSRKLV SRVTSSR+YAAKDI FG GARAAMLQGVSE+AEAVKVT
Sbjct: 274 MYRVVSRLA----SSTSRKLVCSRVTSSRSYAAKDINFGNGARAAMLQGVSEIAEAVKVT 333

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI+FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 334 MGPKGRNVIIDKSHGSPKVTKDGVTVAKSIQFKDKAKNVGADLVKQVASATNTAAGDGTT 393

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKS+ALMISTPEEITQVATI
Sbjct: 394 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSKALMISTPEEITQVATI 453

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 454 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 513

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKC+LENPLILIHEKKISDMNL+LRVLELAVEKKR+LLVVAEDVESDALAMLILNKHH G
Sbjct: 514 QKCDLENPLILIHEKKISDMNLILRVLELAVEKKRSLLVVAEDVESDALAMLILNKHHAG 573

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERGLTLDKVQ++MLG         DDT
Sbjct: 574 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGLTLDKVQVEMLGTAKKVTVSLDDT 633

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 634 IILHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 693

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDA+NATRAAVEEGIVPGGG ALLHATKVL++LQAQN DQKRGIEIVQHALRAP
Sbjct: 694 ERKDRVTDAVNATRAAVEEGIVPGGGAALLHATKVLDDLQAQNADQKRGIEIVQHALRAP 753

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRNLG+DAAKG YVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 754 TFTIVSNAGYDGALVLGKLLEQDDRNLGYDAAKGVYVDMVKAGIVDPLKVVRTALVDAAS 813

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           +S+LLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY
Sbjct: 814 ISMLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 843

BLAST of Sgr025295 vs. NCBI nr
Match: XP_022149451.1 (chaperonin CPN60-like 2, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 993.0 bits (2566), Expect = 1.0e-285
Identity = 539/575 (93.74%), Postives = 548/575 (95.30%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRVASK A    SSTSRKLV SRVTSSRNYAAKDI FG GARAAML+GVSEVAEAVKVT
Sbjct: 1   MYRVASKFA----SSTSRKLVCSRVTSSRNYAAKDINFGDGARAAMLRGVSEVAEAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVAKSINFKDKAKNVGADLVKQVASATNTAAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI
Sbjct: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCELENPLILIHEKKISD+NLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHH G
Sbjct: 241 QKCELENPLILIHEKKISDLNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHAG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGE-VITDERGLTLDKVQIDMLGYCLS-----DD 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGE VITDERGLTLDKVQ++MLG         DD
Sbjct: 301 LKVCAIKAPGFGDNRKANLDDLAILTGGEQVITDERGLTLDKVQVEMLGTAKKVTVSLDD 360

Query: 361 TIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEV 420
           TI+LHGGGDKKLIEERCEQLRTTID S AMFDK+KAQERLSKLSGGVAVFKVGGVSEAEV
Sbjct: 361 TIILHGGGDKKLIEERCEQLRTTIDNSAAMFDKDKAQERLSKLSGGVAVFKVGGVSEAEV 420

Query: 421 GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRA 480
           GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVL+ELQAQNEDQKRGIEIVQ ALRA
Sbjct: 421 GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLDELQAQNEDQKRGIEIVQQALRA 480

Query: 481 PMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAA 540
           P FTIVSNAGYDGALVLGKLLEQDDRN G+DAAKGAYVDMVKAGIVDPLKVVRTALVDAA
Sbjct: 481 PTFTIVSNAGYDGALVLGKLLEQDDRNFGYDAAKGAYVDMVKAGIVDPLKVVRTALVDAA 540

Query: 541 SVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           SVSLLLTTAEAAIVEDPNDKN LPSRMPAMDDMGY
Sbjct: 541 SVSLLLTTAEAAIVEDPNDKNTLPSRMPAMDDMGY 571

BLAST of Sgr025295 vs. ExPASy Swiss-Prot
Match: Q93ZM7 (Chaperonin CPN60-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g13860 PE=1 SV=2)

HSP 1 Score: 850.5 bits (2196), Expect = 1.1e-245
Identity = 444/573 (77.49%), Postives = 512/573 (89.35%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV SKL+SSIGSSTSRKLV  R+ SSRNYAAKDI FG+GARAAMLQGVSEVAEAVKVT
Sbjct: 1   MYRVLSKLSSSIGSSTSRKLVSGRIISSRNYAAKDISFGIGARAAMLQGVSEVAEAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVII+ S+G PK+TKDGVTVA SI F+ KAKN+GA+LVKQVASATN  AGDGTT
Sbjct: 61  MGPKGRNVIIESSYGGPKITKDGVTVAKSISFQAKAKNIGAELVKQVASATNKVAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAIL EGCKS+AAGV+VMDLR+GI  A+ AV+S+LKSRA+MISTPEEITQVATI
Sbjct: 121 CATVLTQAILIEGCKSVAAGVNVMDLRVGINMAIAAVVSDLKSRAVMISTPEEITQVATI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVG+EGVITV+DGNTL++ELEVVEGMKL RG+ISPYFI D+KT
Sbjct: 181 SANGEREIGELIARAMEKVGKEGVITVADGNTLDNELEVVEGMKLARGYISPYFITDEKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCELENP+ILIHEKKISD+N LL+VLE AV+  R LL+VAEDVESDALAMLILNKHH G
Sbjct: 241 QKCELENPIILIHEKKISDINSLLKVLEAAVKSSRPLLIVAEDVESDALAMLILNKHHGG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LSDDT 360
           LKVCAIKAPGFGDNR+A+LDDLA+LTG EVI++ERGL+L+K++ ++LG         DDT
Sbjct: 301 LKVCAIKAPGFGDNRKASLDDLAVLTGAEVISEERGLSLEKIRPELLGTAKKVTVTRDDT 360

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCE+LR+  ++ST+ FD+EK QERLSKLSGGVAVFKVGG SE+EVG
Sbjct: 361 IILHGGGDKKLIEERCEELRSANEKSTSTFDQEKTQERLSKLSGGVAVFKVGGASESEVG 420

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDALNATRAAVEEGI+PGGGVALL+ATK L+ LQ +NEDQ+RG++IVQ+AL+AP
Sbjct: 421 ERKDRVTDALNATRAAVEEGIIPGGGVALLYATKALDNLQTENEDQRRGVQIVQNALKAP 480

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTI +NAGYDG+LV+GKLLEQDD N GFDAAKG YVDMVKAGI+DP+KV+RTAL DAAS
Sbjct: 481 AFTIAANAGYDGSLVVGKLLEQDDCNFGFDAAKGKYVDMVKAGIIDPVKVIRTALTDAAS 540

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMG 569
           VSLLLTT EA+++   ++    P+ +P M  MG
Sbjct: 541 VSLLLTTTEASVLVKADENT--PNHVPDMASMG 571

BLAST of Sgr025295 vs. ExPASy Swiss-Prot
Match: P29197 (Chaperonin CPN60, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CPN60 PE=1 SV=2)

HSP 1 Score: 752.7 bits (1942), Expect = 3.1e-216
Identity = 397/573 (69.28%), Postives = 481/573 (83.94%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYR AS LAS    + + + V SR++ SRNYAAK+IKFGV ARA ML+GV ++A+AVKVT
Sbjct: 1   MYRFASNLASKARIAQNARQVSSRMSWSRNYAAKEIKFGVEARALMLKGVEDLADAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNV+I++S G+PKVTKDGVTVA SI+FKDK KNVGA LVKQVA+ATN  AGDGTT
Sbjct: 61  MGPKGRNVVIEQSWGAPKVTKDGVTVAKSIEFKDKIKNVGASLVKQVANATNDVAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLT+AI  EGCKS+AAG++ MDLR GI  AVDAV++ LKS+A MIST EEI QV TI
Sbjct: 121 CATVLTRAIFAEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKSKARMISTSEEIAQVGTI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIA+AMEKVG+EGVIT+ DG TL +ELEVVEGMKL RG+ SPYFI +QKT
Sbjct: 181 SANGEREIGELIAKAMEKVGKEGVITIQDGKTLFNELEVVEGMKLDRGYTSPYFITNQKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCEL++PLILIHEKKIS +N +++VLELA++++R LL+V+EDVESDALA LILNK   G
Sbjct: 241 QKCELDDPLILIHEKKISSINSIVKVLELALKRQRPLLIVSEDVESDALATLILNKLRAG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LSDDT 360
           +KVCAIKAPGFG+NR+ANL DLA LTGGEVITDE G+ L+KV + MLG C       DDT
Sbjct: 301 IKVCAIKAPGFGENRKANLQDLAALTGGEVITDELGMNLEKVDLSMLGTCKKVTVSKDDT 360

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           ++L G GDKK IEERCEQ+R+ I+ ST+ +DKEK QERL+KLSGGVAV K+GG SEAEVG
Sbjct: 361 VILDGAGDKKGIEERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEVG 420

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           E+KDRVTDALNAT+AAVEEGI+PGGGVALL+A + LE+L   N DQK G++I+Q+AL+ P
Sbjct: 421 EKKDRVTDALNATKAAVEEGILPGGGVALLYAARELEKLPTANFDQKIGVQIIQNALKTP 480

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
           ++TI SNAG +GA+++GKLLEQD+ +LG+DAAKG YVDMVKAGI+DPLKV+RTALVDAAS
Sbjct: 481 VYTIASNAGVEGAVIVGKLLEQDNPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAAS 540

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMG 569
           VS LLTT EA +V+ P D+++  +    M  MG
Sbjct: 541 VSSLLTTTEAVVVDLPKDESESGAAGAGMGGMG 573

BLAST of Sgr025295 vs. ExPASy Swiss-Prot
Match: Q05046 (Chaperonin CPN60-2, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-2 PE=1 SV=1)

HSP 1 Score: 750.0 bits (1935), Expect = 2.0e-215
Identity = 394/575 (68.52%), Postives = 482/575 (83.83%), Query Frame = 0

Query: 1   MYRVASKLASSIG-SSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKV 60
           M+R AS LAS    +      + SR + SRNYAAKD+KFGV AR  ML+GV ++A+AVKV
Sbjct: 1   MHRFASGLASKARLARKGANQIASRSSWSRNYAAKDVKFGVEARGLMLKGVEDLADAVKV 60

Query: 61  TMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGT 120
           TMGPKGRNV+I++S+G+PKVTKDGVTVA SI+FKDK KNVGA LVKQVA+ATN  AGDGT
Sbjct: 61  TMGPKGRNVVIEQSYGAPKVTKDGVTVAKSIEFKDKVKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVAT 180
           TCAT+LT+AI TEGCKS+AAG++ MDLR GI  AVD+V++ LKSRA MIST EEI QV T
Sbjct: 121 TCATILTRAIFTEGCKSVAAGMNAMDLRRGISMAVDSVVTNLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQK 240
           ISANGEREIGELIA+AMEKVG+EGVIT+SDG TL +ELEVVEGMKL RG+ISPYFI +QK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGKTLFNELEVVEGMKLDRGYISPYFITNQK 240

Query: 241 TQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHT 300
            QKCEL++PLILIHEKKIS +N +++VLELA++++R LL+V+EDVESDALA LILNK   
Sbjct: 241 NQKCELDDPLILIHEKKISSINSVVKVLELALKRQRPLLIVSEDVESDALATLILNKLRA 300

Query: 301 GLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LSDD 360
           G+KVCAIKAPGFG+NR+A L DLA+LTGG++IT+E G+ L+KV +DMLG C       DD
Sbjct: 301 GIKVCAIKAPGFGENRKAGLHDLAVLTGGQLITEELGMNLEKVDLDMLGSCKKITISKDD 360

Query: 361 TIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEV 420
           T++L G GDKK IEERCEQ+R+ I+ ST+ +DKEK QERL+KLSGGVAV K+GG SEAEV
Sbjct: 361 TVILDGAGDKKSIEERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRA 480
           GE+KDRVTDALNAT+AAVEEGIVPGGGVALL+A+K L++L   N DQK G++I+Q+AL+ 
Sbjct: 421 GEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLSTANFDQKIGVQIIQNALKT 480

Query: 481 PMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAA 540
           P+ TI SNAG +GA+V+GKLLEQD+ +LG+DAAKG YVDM+KAGI+DPLKV+RTALVDAA
Sbjct: 481 PVHTIASNAGVEGAVVVGKLLEQDNPDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           SVS L+TT EA +VE P D+ ++P+    M  M Y
Sbjct: 541 SVSSLMTTTEAIVVELPKDEKEVPAMGGGMGGMDY 575

BLAST of Sgr025295 vs. ExPASy Swiss-Prot
Match: Q05045 (Chaperonin CPN60-1, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-1 PE=1 SV=1)

HSP 1 Score: 743.8 bits (1919), Expect = 1.4e-213
Identity = 389/575 (67.65%), Postives = 479/575 (83.30%), Query Frame = 0

Query: 1   MYRVASKLASSIG-SSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKV 60
           M+R A+ LAS    +      + SR    RNYAAKD+KFGV AR  ML+GV ++A+AVKV
Sbjct: 1   MHRFATGLASKARLARNGANQIASRSNWRRNYAAKDVKFGVEARGLMLKGVEDLADAVKV 60

Query: 61  TMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGT 120
           TMGPKGR V+I++S G+PKVTKDGVTVA SI+FKDK KNVGA LVKQVA+ATN  AGDGT
Sbjct: 61  TMGPKGRTVVIEQSFGAPKVTKDGVTVAKSIEFKDKVKNVGASLVKQVANATNDVAGDGT 120

Query: 121 TCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVAT 180
           TCAT+LT+AI TEGCKS+A+G++ MDLR GI  AVD+V++ LKSRA MIST EEI QV T
Sbjct: 121 TCATILTKAIFTEGCKSVASGMNAMDLRRGISMAVDSVVTNLKSRARMISTSEEIAQVGT 180

Query: 181 ISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQK 240
           ISANGEREIGELIA+AMEKVG+EGVIT+SDG T+++ELEVVEGMKL RG+ISPYFI +QK
Sbjct: 181 ISANGEREIGELIAKAMEKVGKEGVITISDGKTMDNELEVVEGMKLDRGYISPYFITNQK 240

Query: 241 TQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHT 300
            QKCEL++PLI+I+EKKIS +N +++VLELA++K+R LL+V+EDVES+ALA LILNK   
Sbjct: 241 NQKCELDDPLIIIYEKKISSINAVVKVLELALKKQRPLLIVSEDVESEALATLILNKLRA 300

Query: 301 GLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LSDD 360
           G+KVCAIKAPGFG+NR+A L DLA+LTGG+VIT+E G+ L+KV +DMLG C       DD
Sbjct: 301 GIKVCAIKAPGFGENRKAGLQDLAVLTGGQVITEELGMNLEKVDLDMLGSCKKITISKDD 360

Query: 361 TIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEV 420
           T++L G GDKK IEERC+Q+R+ I+ ST+ +DKEK QERL+KLSGGVAV K+GG SEAEV
Sbjct: 361 TVILDGAGDKKAIEERCDQIRSGIEASTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEV 420

Query: 421 GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRA 480
           GE+KDRVTDALNAT+AAVEEGIVPGGGVALL+A+K L++L   N DQK G++I+Q+AL+ 
Sbjct: 421 GEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLPTANFDQKIGVQIIQNALKT 480

Query: 481 PMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAA 540
           P+ TI SNAG +GA+V+GKLLEQDD +LG+DAAKG YVDMVKAGI+DPLKV+RTALVDAA
Sbjct: 481 PVHTIASNAGVEGAVVVGKLLEQDDPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAA 540

Query: 541 SVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           SVS L+TT E  +VE P D+N++P+    M  M Y
Sbjct: 541 SVSSLMTTTEVVVVELPKDENEVPAMGGGMGGMDY 575

BLAST of Sgr025295 vs. ExPASy Swiss-Prot
Match: P29185 (Chaperonin CPN60-1, mitochondrial OS=Zea mays OX=4577 GN=CPN60I PE=1 SV=2)

HSP 1 Score: 743.4 bits (1918), Expect = 1.9e-213
Identity = 392/577 (67.94%), Postives = 480/577 (83.19%), Query Frame = 0

Query: 1   MYRVASKLAS---SIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAV 60
           MYR A+ LAS     G+S + + V SR+  SRNYAAKDIKFGV ARA ML+GV E+A+AV
Sbjct: 1   MYRAAASLASKARQAGNSLATRQVGSRLAWSRNYAAKDIKFGVEARALMLRGVEELADAV 60

Query: 61  KVTMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGD 120
           KVTMGPKGRNV+I++S G+PKVTKDGVTVA SI+FKD+ KNVGA LVKQVA+ATN  AGD
Sbjct: 61  KVTMGPKGRNVVIEQSFGAPKVTKDGVTVAKSIEFKDRVKNVGASLVKQVANATNDTAGD 120

Query: 121 GTTCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQV 180
           GTTCATVLT+AI TEGCKS+AAG++ MDLR GI  AVDAV++ LK  A MIST EEI QV
Sbjct: 121 GTTCATVLTKAIFTEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKGMARMISTSEEIAQV 180

Query: 181 ATISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFIND 240
            TISANGEREIGELIA+AMEKVG+EGVIT++DGNTL +ELEVVEGMKL RG+ISPYFI +
Sbjct: 181 GTISANGEREIGELIAKAMEKVGKEGVITIADGNTLYNELEVVEGMKLDRGYISPYFITN 240

Query: 241 QKTQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKH 300
            KTQKCELE+PLILIH+KK+++M+ +++VLE+A++K++ LL+VAEDVES+AL  LI+NK 
Sbjct: 241 SKTQKCELEDPLILIHDKKVTNMHAVVKVLEMALKKQKPLLIVAEDVESEALGTLIINKL 300

Query: 301 HTGLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LS 360
             G+KVCA+KAPGFG+NR+ANL DLAILTGGEVIT+E G+ L+  +  MLG C       
Sbjct: 301 RAGIKVCAVKAPGFGENRKANLQDLAILTGGEVITEELGMNLENFEPHMLGTCKKVTVSK 360

Query: 361 DDTIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEA 420
           DDT++L G GDKK IEER EQ+R+ I+ ST+ +DKEK QERL+KLSGGVAV K+GG SEA
Sbjct: 361 DDTVILDGAGDKKSIEERAEQIRSAIENSTSDYDKEKLQERLAKLSGGVAVLKIGGASEA 420

Query: 421 EVGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHAL 480
           EVGE+KDRVTDALNAT+AAVEEGIVPGGGVALL+A+K L++LQ  N DQK G++I+Q+AL
Sbjct: 421 EVGEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELDKLQTANFDQKIGVQIIQNAL 480

Query: 481 RAPMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVD 540
           + P+ TI SNAG +GA+V+GKLLEQ++ +LG+DAAKG YVDMVK GI+DPLKV+RTALVD
Sbjct: 481 KTPVHTIASNAGVEGAVVVGKLLEQENTDLGYDAAKGEYVDMVKTGIIDPLKVIRTALVD 540

Query: 541 AASVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           AASVS L+TT E+ IVE P ++   P+    M  M Y
Sbjct: 541 AASVSSLMTTTESIIVEIPKEEAPAPAMGGGMGGMDY 577

BLAST of Sgr025295 vs. ExPASy TrEMBL
Match: A0A6J1JVR9 (LOW QUALITY PROTEIN: chaperonin CPN60-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111488761 PE=3 SV=1)

HSP 1 Score: 999.6 bits (2583), Expect = 5.4e-288
Identity = 535/574 (93.21%), Postives = 552/574 (96.17%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV SKLA    SSTSRKLV SRVTSSR+YAAKDI FG GARAAMLQGVSE+AEAVKVT
Sbjct: 275 MYRVVSKLA----SSTSRKLVCSRVTSSRSYAAKDINFGNGARAAMLQGVSEIAEAVKVT 334

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDG+TVA SI+FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 335 MGPKGRNVIIDKSHGSPKVTKDGITVAKSIQFKDKAKNVGADLVKQVASATNTAAGDGTT 394

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI
Sbjct: 395 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 454

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 455 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 514

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKC+LENPLILIHEKKISDMNL+LRVLELAVEKKR+LLVVAEDVESDALAMLILNKHH G
Sbjct: 515 QKCDLENPLILIHEKKISDMNLILRVLELAVEKKRSLLVVAEDVESDALAMLILNKHHAG 574

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERGLTLDKVQ++MLG         DDT
Sbjct: 575 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGLTLDKVQVEMLGTAKKVTVSLDDT 634

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 635 IILHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 694

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDA+NATRAAVEEGIVPGGGVALLHATKVL++LQAQN DQKRGIEIVQHALRAP
Sbjct: 695 ERKDRVTDAVNATRAAVEEGIVPGGGVALLHATKVLDDLQAQNADQKRGIEIVQHALRAP 754

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRNLG+DAAKG YVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 755 TFTIVSNAGYDGALVLGKLLEQDDRNLGYDAAKGVYVDMVKAGIVDPLKVVRTALVDAAS 814

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           +S+LLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY
Sbjct: 815 ISMLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 844

BLAST of Sgr025295 vs. ExPASy TrEMBL
Match: A0A6J1D741 (chaperonin CPN60-like 2, mitochondrial isoform X2 OS=Momordica charantia OX=3673 GN=LOC111017875 PE=3 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 2.0e-287
Identity = 539/574 (93.90%), Postives = 548/574 (95.47%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRVASK A    SSTSRKLV SRVTSSRNYAAKDI FG GARAAML+GVSEVAEAVKVT
Sbjct: 1   MYRVASKFA----SSTSRKLVCSRVTSSRNYAAKDINFGDGARAAMLRGVSEVAEAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVAKSINFKDKAKNVGADLVKQVASATNTAAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI
Sbjct: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCELENPLILIHEKKISD+NLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHH G
Sbjct: 241 QKCELENPLILIHEKKISDLNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHAG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERGLTLDKVQ++MLG         DDT
Sbjct: 301 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGLTLDKVQVEMLGTAKKVTVSLDDT 360

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCEQLRTTID S AMFDK+KAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 361 IILHGGGDKKLIEERCEQLRTTIDNSAAMFDKDKAQERLSKLSGGVAVFKVGGVSEAEVG 420

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVL+ELQAQNEDQKRGIEIVQ ALRAP
Sbjct: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLDELQAQNEDQKRGIEIVQQALRAP 480

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRN G+DAAKGAYVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 481 TFTIVSNAGYDGALVLGKLLEQDDRNFGYDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           VSLLLTTAEAAIVEDPNDKN LPSRMPAMDDMGY
Sbjct: 541 VSLLLTTAEAAIVEDPNDKNTLPSRMPAMDDMGY 570

BLAST of Sgr025295 vs. ExPASy TrEMBL
Match: A0A6J1D8E5 (chaperonin CPN60-like 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673 GN=LOC111017875 PE=3 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 5.0e-286
Identity = 539/575 (93.74%), Postives = 548/575 (95.30%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRVASK A    SSTSRKLV SRVTSSRNYAAKDI FG GARAAML+GVSEVAEAVKVT
Sbjct: 1   MYRVASKFA----SSTSRKLVCSRVTSSRNYAAKDINFGDGARAAMLRGVSEVAEAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVAKSINFKDKAKNVGADLVKQVASATNTAAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI
Sbjct: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCELENPLILIHEKKISD+NLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHH G
Sbjct: 241 QKCELENPLILIHEKKISDLNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHAG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGE-VITDERGLTLDKVQIDMLGYCLS-----DD 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGE VITDERGLTLDKVQ++MLG         DD
Sbjct: 301 LKVCAIKAPGFGDNRKANLDDLAILTGGEQVITDERGLTLDKVQVEMLGTAKKVTVSLDD 360

Query: 361 TIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEV 420
           TI+LHGGGDKKLIEERCEQLRTTID S AMFDK+KAQERLSKLSGGVAVFKVGGVSEAEV
Sbjct: 361 TIILHGGGDKKLIEERCEQLRTTIDNSAAMFDKDKAQERLSKLSGGVAVFKVGGVSEAEV 420

Query: 421 GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRA 480
           GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVL+ELQAQNEDQKRGIEIVQ ALRA
Sbjct: 421 GERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLDELQAQNEDQKRGIEIVQQALRA 480

Query: 481 PMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAA 540
           P FTIVSNAGYDGALVLGKLLEQDDRN G+DAAKGAYVDMVKAGIVDPLKVVRTALVDAA
Sbjct: 481 PTFTIVSNAGYDGALVLGKLLEQDDRNFGYDAAKGAYVDMVKAGIVDPLKVVRTALVDAA 540

Query: 541 SVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           SVSLLLTTAEAAIVEDPNDKN LPSRMPAMDDMGY
Sbjct: 541 SVSLLLTTAEAAIVEDPNDKNTLPSRMPAMDDMGY 571

BLAST of Sgr025295 vs. ExPASy TrEMBL
Match: A0A6J1GMW7 (chaperonin CPN60-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455859 PE=3 SV=1)

HSP 1 Score: 992.6 bits (2565), Expect = 6.5e-286
Identity = 530/574 (92.33%), Postives = 552/574 (96.17%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV SKLA    SSTSRKLV SRVTSSR+YAAKDI FG GARAAMLQGVSE+AEAVKVT
Sbjct: 276 MYRVVSKLA----SSTSRKLVCSRVTSSRSYAAKDINFGNGARAAMLQGVSEIAEAVKVT 335

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVIIDKSHGSPKVTKDGVTVA SI+FKDKAKNVGADLVKQVASATNTAAGDGTT
Sbjct: 336 MGPKGRNVIIDKSHGSPKVTKDGVTVAKSIQFKDKAKNVGADLVKQVASATNTAAGDGTT 395

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKS+ALMISTPEEITQVATI
Sbjct: 396 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSKALMISTPEEITQVATI 455

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT
Sbjct: 456 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 515

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKC+LENPLILIHEKKISDMNL+LRVLELAVEKKR+LLVVAEDVESDALAMLILNKHH G
Sbjct: 516 QKCDLENPLILIHEKKISDMNLILRVLELAVEKKRSLLVVAEDVESDALAMLILNKHHAG 575

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----DDT 360
           LKVCAIKAPGFGDNR+ANLDDLAILTGGEVITDERG+TLDKVQ++MLG         DDT
Sbjct: 576 LKVCAIKAPGFGDNRKANLDDLAILTGGEVITDERGITLDKVQVEMLGTAKKVTVSLDDT 635

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           ++LHGGGDKKLIEERCEQLRTTID+STAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG
Sbjct: 636 LILHGGGDKKLIEERCEQLRTTIDKSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 695

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDA+NATRAAVEEGIVPGGGVALLHATKVL++LQAQN DQKRGIEIVQHALRAP
Sbjct: 696 ERKDRVTDAVNATRAAVEEGIVPGGGVALLHATKVLDDLQAQNADQKRGIEIVQHALRAP 755

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTIVSNAGYDGALVLGKLLEQDDRNLG+DAAKG YVDMVKAGIVDPLKVVRTALVDAAS
Sbjct: 756 TFTIVSNAGYDGALVLGKLLEQDDRNLGYDAAKGVYVDMVKAGIVDPLKVVRTALVDAAS 815

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           +S+LLTTAEAAIVEDP+DKNKLPSR+PAMDDMGY
Sbjct: 816 ISMLLTTAEAAIVEDPDDKNKLPSRIPAMDDMGY 845

BLAST of Sgr025295 vs. ExPASy TrEMBL
Match: A0A5D3C0C6 (Chaperonin CPN60-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00910 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.8e-276
Identity = 521/576 (90.45%), Postives = 539/576 (93.58%), Query Frame = 0

Query: 1   MYRVAS--KLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVK 60
           MYR+AS  KLASS GSSTSRKLV SRVTSSR+Y AKDI FG GARAAMLQGVSEVAEAVK
Sbjct: 1   MYRLASKLKLASSFGSSTSRKLVCSRVTSSRSYVAKDINFGNGARAAMLQGVSEVAEAVK 60

Query: 61  VTMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDG 120
           VTMGPKGRNVIID   GSPKVTKDGVTVA SI+FKDKAKNVGADLVKQVASATNTAAGDG
Sbjct: 61  VTMGPKGRNVIIDSRLGSPKVTKDGVTVAKSIQFKDKAKNVGADLVKQVASATNTAAGDG 120

Query: 121 TTCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVA 180
           TTCATVLTQAILTEGCKSIAAGV+VMDLRIGIK AVDAVISELKS ALMISTPEEITQVA
Sbjct: 121 TTCATVLTQAILTEGCKSIAAGVNVMDLRIGIKKAVDAVISELKSTALMISTPEEITQVA 180

Query: 181 TISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQ 240
           TISANGEREIGEL+ARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQ
Sbjct: 181 TISANGEREIGELLARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQ 240

Query: 241 KTQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHH 300
           K+QKCELENP ILIHEKKISDMNLLLR LELAV  KRALLVVAEDVESDALAMLILNKH 
Sbjct: 241 KSQKCELENPFILIHEKKISDMNLLLRALELAVTNKRALLVVAEDVESDALAMLILNKHR 300

Query: 301 TGLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYCLS-----D 360
            GLKVCAIKAPGFGDNRRANLDDL+ILTGGEVIT+ERGLTLDKVQ++MLG         D
Sbjct: 301 AGLKVCAIKAPGFGDNRRANLDDLSILTGGEVITNERGLTLDKVQVEMLGTAKKVTVSLD 360

Query: 361 DTIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAE 420
           DTI+LHGGGDKKLIEERCEQLRT+ID+STAMFDKEKAQERLSKLSGGVAVFKVGGVSEAE
Sbjct: 361 DTIILHGGGDKKLIEERCEQLRTSIDKSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAE 420

Query: 421 VGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALR 480
           VGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVL+ELQAQNEDQKRGIEIVQHALR
Sbjct: 421 VGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLDELQAQNEDQKRGIEIVQHALR 480

Query: 481 APMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDA 540
           AP   IVSNAGYDGALV+GKLLEQDDRNLGFDAAKG YVDMVKAGIVDPLKVVRTALVDA
Sbjct: 481 APTSAIVSNAGYDGALVVGKLLEQDDRNLGFDAAKGVYVDMVKAGIVDPLKVVRTALVDA 540

Query: 541 ASVSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMGY 570
           +SVSLLL TAEAAIVE PN  NKLPSRMPAM+DM +
Sbjct: 541 SSVSLLLATAEAAIVEHPNKTNKLPSRMPAMNDMNF 576

BLAST of Sgr025295 vs. TAIR 10
Match: AT3G13860.1 (heat shock protein 60-3A )

HSP 1 Score: 850.5 bits (2196), Expect = 7.7e-247
Identity = 444/573 (77.49%), Postives = 512/573 (89.35%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYRV SKL+SSIGSSTSRKLV  R+ SSRNYAAKDI FG+GARAAMLQGVSEVAEAVKVT
Sbjct: 1   MYRVLSKLSSSIGSSTSRKLVSGRIISSRNYAAKDISFGIGARAAMLQGVSEVAEAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNVII+ S+G PK+TKDGVTVA SI F+ KAKN+GA+LVKQVASATN  AGDGTT
Sbjct: 61  MGPKGRNVIIESSYGGPKITKDGVTVAKSISFQAKAKNIGAELVKQVASATNKVAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLTQAIL EGCKS+AAGV+VMDLR+GI  A+ AV+S+LKSRA+MISTPEEITQVATI
Sbjct: 121 CATVLTQAILIEGCKSVAAGVNVMDLRVGINMAIAAVVSDLKSRAVMISTPEEITQVATI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIARAMEKVG+EGVITV+DGNTL++ELEVVEGMKL RG+ISPYFI D+KT
Sbjct: 181 SANGEREIGELIARAMEKVGKEGVITVADGNTLDNELEVVEGMKLARGYISPYFITDEKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCELENP+ILIHEKKISD+N LL+VLE AV+  R LL+VAEDVESDALAMLILNKHH G
Sbjct: 241 QKCELENPIILIHEKKISDINSLLKVLEAAVKSSRPLLIVAEDVESDALAMLILNKHHGG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LSDDT 360
           LKVCAIKAPGFGDNR+A+LDDLA+LTG EVI++ERGL+L+K++ ++LG         DDT
Sbjct: 301 LKVCAIKAPGFGDNRKASLDDLAVLTGAEVISEERGLSLEKIRPELLGTAKKVTVTRDDT 360

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           I+LHGGGDKKLIEERCE+LR+  ++ST+ FD+EK QERLSKLSGGVAVFKVGG SE+EVG
Sbjct: 361 IILHGGGDKKLIEERCEELRSANEKSTSTFDQEKTQERLSKLSGGVAVFKVGGASESEVG 420

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           ERKDRVTDALNATRAAVEEGI+PGGGVALL+ATK L+ LQ +NEDQ+RG++IVQ+AL+AP
Sbjct: 421 ERKDRVTDALNATRAAVEEGIIPGGGVALLYATKALDNLQTENEDQRRGVQIVQNALKAP 480

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
            FTI +NAGYDG+LV+GKLLEQDD N GFDAAKG YVDMVKAGI+DP+KV+RTAL DAAS
Sbjct: 481 AFTIAANAGYDGSLVVGKLLEQDDCNFGFDAAKGKYVDMVKAGIIDPVKVIRTALTDAAS 540

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMG 569
           VSLLLTT EA+++   ++    P+ +P M  MG
Sbjct: 541 VSLLLTTTEASVLVKADENT--PNHVPDMASMG 571

BLAST of Sgr025295 vs. TAIR 10
Match: AT3G23990.1 (heat shock protein 60 )

HSP 1 Score: 752.7 bits (1942), Expect = 2.2e-217
Identity = 397/573 (69.28%), Postives = 481/573 (83.94%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKLVFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAVKVT 60
           MYR AS LAS    + + + V SR++ SRNYAAK+IKFGV ARA ML+GV ++A+AVKVT
Sbjct: 1   MYRFASNLASKARIAQNARQVSSRMSWSRNYAAKEIKFGVEARALMLKGVEDLADAVKVT 60

Query: 61  MGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGDGTT 120
           MGPKGRNV+I++S G+PKVTKDGVTVA SI+FKDK KNVGA LVKQVA+ATN  AGDGTT
Sbjct: 61  MGPKGRNVVIEQSWGAPKVTKDGVTVAKSIEFKDKIKNVGASLVKQVANATNDVAGDGTT 120

Query: 121 CATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQVATI 180
           CATVLT+AI  EGCKS+AAG++ MDLR GI  AVDAV++ LKS+A MIST EEI QV TI
Sbjct: 121 CATVLTRAIFAEGCKSVAAGMNAMDLRRGISMAVDAVVTNLKSKARMISTSEEIAQVGTI 180

Query: 181 SANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFINDQKT 240
           SANGEREIGELIA+AMEKVG+EGVIT+ DG TL +ELEVVEGMKL RG+ SPYFI +QKT
Sbjct: 181 SANGEREIGELIAKAMEKVGKEGVITIQDGKTLFNELEVVEGMKLDRGYTSPYFITNQKT 240

Query: 241 QKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKHHTG 300
           QKCEL++PLILIHEKKIS +N +++VLELA++++R LL+V+EDVESDALA LILNK   G
Sbjct: 241 QKCELDDPLILIHEKKISSINSIVKVLELALKRQRPLLIVSEDVESDALATLILNKLRAG 300

Query: 301 LKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LSDDT 360
           +KVCAIKAPGFG+NR+ANL DLA LTGGEVITDE G+ L+KV + MLG C       DDT
Sbjct: 301 IKVCAIKAPGFGENRKANLQDLAALTGGEVITDELGMNLEKVDLSMLGTCKKVTVSKDDT 360

Query: 361 IVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEAEVG 420
           ++L G GDKK IEERCEQ+R+ I+ ST+ +DKEK QERL+KLSGGVAV K+GG SEAEVG
Sbjct: 361 VILDGAGDKKGIEERCEQIRSAIELSTSDYDKEKLQERLAKLSGGVAVLKIGGASEAEVG 420

Query: 421 ERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHALRAP 480
           E+KDRVTDALNAT+AAVEEGI+PGGGVALL+A + LE+L   N DQK G++I+Q+AL+ P
Sbjct: 421 EKKDRVTDALNATKAAVEEGILPGGGVALLYAARELEKLPTANFDQKIGVQIIQNALKTP 480

Query: 481 MFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVDAAS 540
           ++TI SNAG +GA+++GKLLEQD+ +LG+DAAKG YVDMVKAGI+DPLKV+RTALVDAAS
Sbjct: 481 VYTIASNAGVEGAVIVGKLLEQDNPDLGYDAAKGEYVDMVKAGIIDPLKVIRTALVDAAS 540

Query: 541 VSLLLTTAEAAIVEDPNDKNKLPSRMPAMDDMG 569
           VS LLTT EA +V+ P D+++  +    M  MG
Sbjct: 541 VSSLLTTTEAVVVDLPKDESESGAAGAGMGGMG 573

BLAST of Sgr025295 vs. TAIR 10
Match: AT2G33210.1 (heat shock protein 60-2 )

HSP 1 Score: 728.0 bits (1878), Expect = 5.8e-210
Identity = 389/580 (67.07%), Postives = 474/580 (81.72%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKL---VFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAV 60
           MYR+ S +AS   +  +RK    + SR+ S+RNYAAKDI+FGV ARA ML+GV ++A+AV
Sbjct: 1   MYRLVSNVASK--ARIARKCTSQIGSRLNSTRNYAAKDIRFGVEARALMLRGVEDLADAV 60

Query: 61  KVTMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGD 120
           KVTMGPKGRNVII++S G+PKVTKDGVTVA SI+FKD+ KNVGA LVKQVA+ATN  AGD
Sbjct: 61  KVTMGPKGRNVIIEQSWGAPKVTKDGVTVAKSIEFKDRIKNVGASLVKQVANATNDVAGD 120

Query: 121 GTTCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQV 180
           GTTCATVLT+AI TEGCKS+AAG++ MDLR GIK AVD V++ L+SRA MIST EEI QV
Sbjct: 121 GTTCATVLTRAIFTEGCKSVAAGMNAMDLRRGIKLAVDTVVTNLQSRARMISTSEEIAQV 180

Query: 181 ATISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFIND 240
            TISANG+REIGELIA+AME VG+EGVIT+ DG TL +ELEVVEGMK+ RG+ISPYFI +
Sbjct: 181 GTISANGDREIGELIAKAMETVGKEGVITIQDGKTLFNELEVVEGMKIDRGYISPYFITN 240

Query: 241 QKTQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKH 300
            KTQKCELE+PLILIHEKKIS++N +++VLELA++K+R LL+VAEDVESDALA LILNK 
Sbjct: 241 PKTQKCELEDPLILIHEKKISNINAMVKVLELALKKQRPLLIVAEDVESDALATLILNKL 300

Query: 301 HTGLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LS 360
              +KVCA+KAPGFG+NR+ANL DLA LTG +VIT+E G+ LD + + M G C       
Sbjct: 301 RANIKVCAVKAPGFGENRKANLHDLAALTGAQVITEELGMNLDNIDLSMFGNCKKVTVSK 360

Query: 361 DDTIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEA 420
           DDT+VL G GDK+ I ERCEQ+R+ ++ ST+ +DKEK QERL+KLSGGVAV K+GG SE 
Sbjct: 361 DDTVVLDGAGDKQAIGERCEQIRSMVEASTSDYDKEKLQERLAKLSGGVAVLKIGGASET 420

Query: 421 EVGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHAL 480
           EV E+KDRVTDALNAT+AAVEEGIVPGGGVALL+A+K LE+L   N DQK G++I+Q+AL
Sbjct: 421 EVSEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELEKLSTANFDQKIGVQIIQNAL 480

Query: 481 RAPMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVD 540
           + P++TI SNAG +GA+V+GKLLEQD+ +LG+DAAKG YVDM+KAGI+DPLKV+RTALVD
Sbjct: 481 KTPVYTIASNAGVEGAVVVGKLLEQDNPDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVD 540

Query: 541 AASVSLLLTTAEAAIVEDPNDKNKLPSR----MPAMDDMG 569
           AASVS LLTT EA + E P  +   P      M  M  MG
Sbjct: 541 AASVSSLLTTTEAVVTEIPTKEVASPGMGGGGMGGMGGMG 578

BLAST of Sgr025295 vs. TAIR 10
Match: AT2G33210.2 (heat shock protein 60-2 )

HSP 1 Score: 712.6 bits (1838), Expect = 2.5e-205
Identity = 386/580 (66.55%), Postives = 469/580 (80.86%), Query Frame = 0

Query: 1   MYRVASKLASSIGSSTSRKL---VFSRVTSSRNYAAKDIKFGVGARAAMLQGVSEVAEAV 60
           MYR+ S +AS   +  +RK    + SR+ S+RNYAAKDI+FGV ARA ML+GV ++A+AV
Sbjct: 1   MYRLVSNVASK--ARIARKCTSQIGSRLNSTRNYAAKDIRFGVEARALMLRGVEDLADAV 60

Query: 61  KVTMGPKGRNVIIDKSHGSPKVTKDGVTVANSIKFKDKAKNVGADLVKQVASATNTAAGD 120
           KVTMGPKGRNVII++S G+PKVTKDGVTVA SI+FKD+ KNVGA LVKQVA+ATN  AGD
Sbjct: 61  KVTMGPKGRNVIIEQSWGAPKVTKDGVTVAKSIEFKDRIKNVGASLVKQVANATNDVAGD 120

Query: 121 GTTCATVLTQAILTEGCKSIAAGVSVMDLRIGIKTAVDAVISELKSRALMISTPEEITQV 180
           GTTCATVLT+AI TEGCKS+AAG++ MDLR GIK AVD V++ L+SRA MIST EEI QV
Sbjct: 121 GTTCATVLTRAIFTEGCKSVAAGMNAMDLRRGIKLAVDTVVTNLQSRARMISTSEEIAQV 180

Query: 181 ATISANGEREIGELIARAMEKVGREGVITVSDGNTLEDELEVVEGMKLGRGFISPYFIND 240
            TISANG+REIGELIA+AME VG+EGVIT+ DG TL +ELEVVEGMK+ RG+ISPYFI +
Sbjct: 181 GTISANGDREIGELIAKAMETVGKEGVITIQDGKTLFNELEVVEGMKIDRGYISPYFITN 240

Query: 241 QKTQKCELENPLILIHEKKISDMNLLLRVLELAVEKKRALLVVAEDVESDALAMLILNKH 300
            KTQKCELE+PLILIHEKKIS++N +++VLELA++K+R LL+VAEDVESDALA LILNK 
Sbjct: 241 PKTQKCELEDPLILIHEKKISNINAMVKVLELALKKQRPLLIVAEDVESDALATLILNKL 300

Query: 301 HTGLKVCAIKAPGFGDNRRANLDDLAILTGGEVITDERGLTLDKVQIDMLGYC-----LS 360
                   IKAPGFG+NR+ANL DLA LTG +VIT+E G+ LD + + M G C       
Sbjct: 301 RAN-----IKAPGFGENRKANLHDLAALTGAQVITEELGMNLDNIDLSMFGNCKKVTVSK 360

Query: 361 DDTIVLHGGGDKKLIEERCEQLRTTIDRSTAMFDKEKAQERLSKLSGGVAVFKVGGVSEA 420
           DDT+VL G GDK+ I ERCEQ+R+ ++ ST+ +DKEK QERL+KLSGGVAV K+GG SE 
Sbjct: 361 DDTVVLDGAGDKQAIGERCEQIRSMVEASTSDYDKEKLQERLAKLSGGVAVLKIGGASET 420

Query: 421 EVGERKDRVTDALNATRAAVEEGIVPGGGVALLHATKVLEELQAQNEDQKRGIEIVQHAL 480
           EV E+KDRVTDALNAT+AAVEEGIVPGGGVALL+A+K LE+L   N DQK G++I+Q+AL
Sbjct: 421 EVSEKKDRVTDALNATKAAVEEGIVPGGGVALLYASKELEKLSTANFDQKIGVQIIQNAL 480

Query: 481 RAPMFTIVSNAGYDGALVLGKLLEQDDRNLGFDAAKGAYVDMVKAGIVDPLKVVRTALVD 540
           + P++TI SNAG +GA+V+GKLLEQD+ +LG+DAAKG YVDM+KAGI+DPLKV+RTALVD
Sbjct: 481 KTPVYTIASNAGVEGAVVVGKLLEQDNPDLGYDAAKGEYVDMIKAGIIDPLKVIRTALVD 540

Query: 541 AASVSLLLTTAEAAIVEDPNDKNKLPSR----MPAMDDMG 569
           AASVS LLTT EA + E P  +   P      M  M  MG
Sbjct: 541 AASVSSLLTTTEAVVTEIPTKEVASPGMGGGGMGGMGGMG 573

BLAST of Sgr025295 vs. TAIR 10
Match: AT3G13470.1 (TCP-1/cpn60 chaperonin family protein )

HSP 1 Score: 413.7 bits (1062), Expect = 2.4e-115
Identity = 235/547 (42.96%), Postives = 354/547 (64.72%), Query Frame = 0

Query: 32  AAKDIKFGV-GARAAMLQ-GVSEVAEAVKVTMGPKGRNVIIDKSHGSPKVTKDGVTVANS 91
           AAK++ F   G     LQ GV+++A+ V VT+GPKGRNV+++  +GSP++  DGVTVA  
Sbjct: 51  AAKELHFNKDGTTIRKLQTGVNKLADLVGVTLGPKGRNVVLESKYGSPRIVNDGVTVARE 110

Query: 92  IKFKDKAKNVGADLVKQVASATNTAAGDGTTCATVLTQAILTEGCKSIAAGVSVMDLRIG 151
           ++ +D  +N+GA LV+Q A+ TN  AGDGTT + VL Q  + EG K +AAG + + +  G
Sbjct: 111 VELEDPVENIGAKLVRQAAAKTNDLAGDGTTTSVVLAQGFIAEGVKVVAAGANPVLITRG 170

Query: 152 IKTAVDAVISELKSRALMISTPEEITQVATISANGEREIGELIARAMEKVGREGVITVSD 211
           I+    A+++ELK  +  +    E+  VA +SA    E+G +IA AM KVGR+GV+T+ +
Sbjct: 171 IEKTAKALVNELKLMSKEVE-DSELADVAAVSAGNNHEVGSMIAEAMSKVGRKGVVTLEE 230

Query: 212 GNTLEDELEVVEGMKLGRGFISPYFINDQKTQKCELENPLILIHEKKISDMNLLLRVLEL 271
           G + E+ L VVEGM+  RG+ISPYF+ D +    E +N  +L+ +KK+++   L+ VLE 
Sbjct: 231 GKSAENNLYVVEGMQFDRGYISPYFVTDSEKMSVEYDNCKLLLVDKKVTNARDLVGVLED 290

Query: 272 AVEKKRALLVVAEDVESDALAMLILNKHHTGLKVCAIKAPGFGDNRRANLDDLAILTGGE 331
           A+     +L++AED+E +ALA L++NK    LK+ A+KAPGFG+ +   LDD+AILTG  
Sbjct: 291 AIRGGYPILIIAEDIEQEALATLVVNKLRGTLKIAALKAPGFGERKSQYLDDIAILTGAT 350

Query: 332 VITDERGLTLDKVQIDMLG-----YCLSDDTIVLHGGGDKKLIEERCEQLRTTIDRSTAM 391
           VI +E GL+LDK   ++LG         + T ++  G  ++ + +R  Q+R  I+++   
Sbjct: 351 VIREEVGLSLDKAGKEVLGNASKVVLTKEMTTIVGDGTTQEAVNKRVVQIRNLIEQAEQD 410

Query: 392 FDKEKAQERLSKLSGGVAVFKVGGVSEAEVGERKDRVTDALNATRAAVEEGIVPGGGVAL 451
           ++KEK  ER++KLSGGVAV +VG  +E E+ E+K RV DALNAT+AAVEEGIV GGG  L
Sbjct: 411 YEKEKLNERIAKLSGGVAVIQVGAQTETELKEKKLRVEDALNATKAAVEEGIVVGGGCTL 470

Query: 452 LHATKVLEELQ--AQNEDQKRGIEIVQHALRAPMFTIVSNAGYDGALVLGKLLEQDDRNL 511
           L     ++ ++   +N+++K G EIV+ AL  P+  I  NAG +G++V  K+L  D+   
Sbjct: 471 LRLASKVDAIKDTLENDEEKVGAEIVKRALSYPLKLIAKNAGVNGSVVSEKVLANDNVKF 530

Query: 512 GFDAAKGAYVDMVKAGIVDPLKVVRTALVDAASVSLLLTTAEAAIVEDPNDKNKLPSRMP 570
           G++AA G Y D++ AGI+DP KVVR  L  AASV+     ++  +VE P +   +P+  P
Sbjct: 531 GYNAATGKYEDLMAAGIIDPTKVVRCCLEHAASVAKTFLMSDCVVVEIP-EPEPVPAGNP 590

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022992465.11.1e-28793.21LOW QUALITY PROTEIN: chaperonin CPN60-like 2, mitochondrial [Cucurbita maxima][more]
KAG6575473.11.4e-28793.21Chaperonin CPN60-like 2, mitochondrial, partial [Cucurbita argyrosperma subsp. s... [more]
XP_022149459.14.2e-28793.90chaperonin CPN60-like 2, mitochondrial isoform X2 [Momordica charantia][more]
XP_023548119.11.2e-28692.86chaperonin CPN60-like 2, mitochondrial [Cucurbita pepo subsp. pepo][more]
XP_022149451.11.0e-28593.74chaperonin CPN60-like 2, mitochondrial isoform X1 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q93ZM71.1e-24577.49Chaperonin CPN60-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At3g13... [more]
P291973.1e-21669.28Chaperonin CPN60, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=CPN60 PE=1 SV... [more]
Q050462.0e-21568.52Chaperonin CPN60-2, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-2 PE=1 SV... [more]
Q050451.4e-21367.65Chaperonin CPN60-1, mitochondrial OS=Cucurbita maxima OX=3661 GN=CPN60-1 PE=1 SV... [more]
P291851.9e-21367.94Chaperonin CPN60-1, mitochondrial OS=Zea mays OX=4577 GN=CPN60I PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1JVR95.4e-28893.21LOW QUALITY PROTEIN: chaperonin CPN60-like 2, mitochondrial OS=Cucurbita maxima ... [more]
A0A6J1D7412.0e-28793.90chaperonin CPN60-like 2, mitochondrial isoform X2 OS=Momordica charantia OX=3673... [more]
A0A6J1D8E55.0e-28693.74chaperonin CPN60-like 2, mitochondrial isoform X1 OS=Momordica charantia OX=3673... [more]
A0A6J1GMW76.5e-28692.33chaperonin CPN60-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A5D3C0C62.8e-27690.45Chaperonin CPN60-like 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold... [more]
Match NameE-valueIdentityDescription
AT3G13860.17.7e-24777.49heat shock protein 60-3A [more]
AT3G23990.12.2e-21769.28heat shock protein 60 [more]
AT2G33210.15.8e-21067.07heat shock protein 60-2 [more]
AT2G33210.22.5e-20566.55heat shock protein 60-2 [more]
AT3G13470.12.4e-11542.96TCP-1/cpn60 chaperonin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 451..471
NoneNo IPR availablePANTHERPTHR45633:SF26CHAPERONIN CPN60-2, MITOCHONDRIALcoord: 19..561
NoneNo IPR availablePANTHERPTHR4563360 KDA HEAT SHOCK PROTEIN, MITOCHONDRIALcoord: 19..561
IPR001844Chaperonin Cpn60PRINTSPR00298CHAPERONIN60coord: 113..140
score: 60.66
coord: 423..444
score: 84.38
coord: 375..400
score: 50.84
coord: 57..83
score: 67.24
coord: 298..321
score: 56.78
IPR001844Chaperonin Cpn60TIGRFAMTIGR02348TIGR02348coord: 33..552
e-value: 1.9E-203
score: 674.7
IPR001844Chaperonin Cpn60CDDcd03344GroELcoord: 34..549
e-value: 0.0
score: 787.805
IPR027409GroEL-like apical domain superfamilyGENE3D3.50.7.10GroELcoord: 222..398
e-value: 1.1E-214
score: 715.6
IPR027409GroEL-like apical domain superfamilySUPERFAMILY52029GroEL apical domain-likecoord: 214..401
IPR002423Chaperonin Cpn60/TCP-1 familyPFAMPF00118Cpn60_TCP1coord: 53..548
e-value: 6.0E-77
score: 259.3
IPR027410TCP-1-like chaperonin intermediate domain superfamilyGENE3D3.30.260.10coord: 167..435
e-value: 1.1E-214
score: 715.6
IPR027410TCP-1-like chaperonin intermediate domain superfamilySUPERFAMILY54849GroEL-intermediate domain likecoord: 168..234
IPR027413GroEL-like equatorial domain superfamilyGENE3D1.10.560.10coord: 36..548
e-value: 1.1E-214
score: 715.6
IPR027413GroEL-like equatorial domain superfamilySUPERFAMILY48592GroEL equatorial domain-likecoord: 36..547
IPR018370Chaperonin Cpn60, conserved sitePROSITEPS00296CHAPERONINS_CPN60coord: 430..441

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025295.1Sgr025295.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042026 protein refolding
biological_process GO:0006457 protein folding
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005739 mitochondrion
molecular_function GO:0005524 ATP binding
molecular_function GO:0016887 ATP hydrolysis activity