Sgr025287 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025287
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionCytochrome p450
Locationtig00004836: 1225709 .. 1231228 (-)
RNA-Seq ExpressionSgr025287
SyntenySgr025287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGTCAGGGCTGTGGTGATGATGATTAAGCATATTTAAGAGAAGTAGAGAGAGAGAGAGAGGGAGAGAGGAGAGGAGAGAGAGAGAGAGACAGGCAGGCAGGGAGGCAGAGGGGGAGCGTGCGGGCAGAGGAATTCTTTGTCCAAAATGGACAGTTTATGGGATGTTGTGGTAGTGACAGCCATTTTCTTGACCACTATTATTGTCTACAGGAACTGCTTTAGACTTCTCAGATCAAACTTCTGCAGCCAACTTCCTCAGGGCACTCTCGGTTGGCCTTTCATTGGTGAGACCATTGAGTTCATCTCCTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGCCGCCGCCTGTAAGTTCTCCAAAAAAAAAAAACCAACCTCAAAAACACTCAAATGACCTTCAAATGCTTGTCTGACTAGTGATTGTTTGTTTGTTAATGGAGTTTGCTTAGCTTCAGTTTCCCCCCCATAAGTTTGTAATTTTCTCTTAAACCAATGCTTCATTTTGGTTTACAGGTATGGGAAGGTGTTCAAGTCTCATATTTTTGGAAGTCCTACCATTGTTTCTACAGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCCAAGGCTTTTGTACCGTCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAATGGGAGCTTGCATAAGAGAATCCATGGTCTTGTGGGAGCTTTCTTCAAGTCTCCACATCTCAAAGCTCAAATAACCAAGGATATGGAGAGTTATGTCAAACTATCAATGGCAAATTGGACAGAGAACAATCCTATTTACCTTCAAGATGAAACCAAAAATGTCAGCTTTCTCTCTCTCTCTCTCCCCTTTTTGGAGCTTGAAATTAACCTGTGAATTTGCTTTTTATTCCCTTACATGAAGTTAACAAAAAGACAAGAGATACAAGTGTCCCACCATTACTGTGTTGATACTAGACAATACTGTGTTGATACTAGACAAAAACAGTTACTTTCTTTACCCGATTCAAGTGAATCATTGATGTTAAAATAATCCATTTCTCTGGTTCCTACTTAGGTTTAAAGATTATTTCTGTCAAAAGTAAGAGAACCCCACAGTTTTTGTTATCATTCTGCACAAATCATTTTGTCCAAGAATTTACATTTGTAATCATTAGCCATCTATTTTCCTCTTCAAAAAGTTACTAAGTGAACCAACCCTTTTGTCTCCAGATTTCCTTTCAAATCTTAGTAAAGATATTGATTAGTTTAAATCCTGGTCAAAGGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCCGGGCTTATGGCTTTCCCAGTGAACATACCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGTCGTAAAAGGTCTACTATCCTCCTTCACTAGTACTTACTTTCTGTGTTCTCAAAATGATGGAAATTCACTTGTTCTCAGGCCAAAAGGAACATGGTTAAATTAGTAAAGAAGATAATACAAGAGAGAAAAAGCAGTGGCATTTCTCCTGTCCCAAAAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGAACAACTAACAGATAATCTTATAGCAGAGAATATGATTGACATGATGATTCCGGGTGAAGATTCAGTCCCAATTTTAATGACCCTTGCAGTAAACTACCTCTCCGATTGCCCGGCTGCACTTCAACATTTAACGGTGCGCTATTTCTCTTTCGAACTCTCGATATTGCCAGTTCTTAAGTGAAAAATCTACATCATACTGAGACGGAATTTACTTTCATTTGGTACACGGCTTTATTTGTTGACTTTAACAATGTCCCTTTCAGCCATGTCTTTGGAGCTTATTTTCTTTGTTCTTTTATGTCTGTCACATGGTCTTCAAGTGCTAGCTAGAACAAACCCGAACCAACTTCTTAATGTACTAATTTTTCTGCAAGTTCATGTAAGATTTAATTTTATTGAATCCATGGCTGGAATATAAATTAAATGCAGACATTATGGTCTTTTAGTAATATTCAGTTTAAAGCAAAGAAGGTTAATCTATGCTTTTCCTTGGCTTCTTTGGATAGTTCTTAACTGGATGCCTGTTTGGATTCATATCAGGAGGAAAACTTAAGGATGAAAAGACTTAAAGAGCAGCTCAGAGAGTCGTTGCATTGGAGCGATTACTTATCGTTGCCATTTACGCAGAGTGTAATCTATTAACTTTATAAAAGAGTAGGAAGGCTGGAATGCTTGCAGTATTAGTGATTATGTTGAATATGTTTTCAATTGAATAGGTGATCACAGAGACTTTAAGGATGGGAAACGTCATAACCGGGGTGATGCGGAAAGCCATGAAAGACATGAAGATAAAAGGATATCTAATACCAAAAGGTTGGTCTGTTCTTACGTACTTTAGATCCGTTCATCTCGACGACAATCACTATGACTGGCCTTACCAATTCAATCCATGGAGATGGCAAGTAAGAAGGAACATATTTCTTCCTCTCTCTTTCTATCTTTTGCTTTTAATGTTTCCCCTTAATGGGTTTGGGGTGTCCAACAGGACAGAGACTTGAGCAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGGCTTGAAGCTTCCATCTTTCTTCATCACTTTGTCACTGAGTTCAGGTAACTGCCAAAATCACTTTTCCCATCTCAACTTTTGACTGTTTTTCTTGTTTCAGTTGGCATGGAAAACAAAACCACTCCCAATAGTTTGGAGCAACATTGACTCCTTAGGCATTTGATTTGGTATCTTGTGTTTTTTTTTTTTATCTTTTTTTATAACGTGGTCTTGAGGGCTCGTTTTTTGCATTATGTTCGTTACCCATTCCAGTAATCTTCGGAGAGTCTTCACCCACTCCAAAGTTCGCTTCAGGAAATAATGAAGATTTTGCATTTTATATTAAATTTTCTTTTTAGTACTTTACACGCTTTTACTAACTTCTCTTGATATATACTTTCTTTATTAATCTCTCTCTTACATGAACTTTCTCTCGTTTGTCCCTTAATGTATACAATCTCACTTATGCTTTTACACTTGCACTCTCTTTATGACGAAGCTCTCTCTATACACTTGATTTGTTACAGATGGATGGTTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGAATGAAGAAGAGGATGCCAGTTTGGGTGAAGAGGAGAAGAGACCCATGAAAAGGGCAAGAGGAGCTCTGCCTTTTTTCCAAAAGCACAACTTCAATTAACTTCACCAATTACACAACAACCAAATATACCTTTGGAAAAAAAAATTCTTACATTTAACAAAGGGAATGTGGGTAAAATAGAGGGCATTTAAAATATGACACTTTTATCCTCTCGTACGCCTATTGCACGTAGAAAGACCAAGAAAATTCCTATAGTTTACTTGCATATCTGTCACGTGTTGTAAATGATATATATATATATATGTATGTACCTATTTGAATATATAGTAGTAGGTCTTAACAAGTAATGTGTAATGTATAATAGAGTTATCAGTTGCATTGTGTTGATCTTTCTGAATATTTTTGTCATTAGTAGATTAACAAGTTTGTATTTTAGTGGAATCAGGTGTTGCAACTTTTTTTTCTTTTTTCATGTAATTGTAAATGTTATTGAAAGAAAAATCATAACAAATACCATAATTTAATACTAAAATTACACGATAGCAATCATTTTTAATATTTAAAAAATTACTGAATGATGTTTCAACAATAATCGAATCCAGTCATTGAGTTGTTGAATTCATACAAATTAAAATATGACACTAAATATCTGTTCTATTAATTAAGCAAGAAAGTTAATTGGGCCTCCGAGCCGAAGTAGTATACTTTTATTATTGGGCCGTATTATTTATTTTGGGCCCTGAGTTAGGGGAATGTTTGGGCCTAACTTGAATCGGGCCCATTTATTGGCCCTAACTTGTAAATAAACTAGTTTTTATTTTCAATTTATAATCTCAAATCCATCATGCTTTAATAAATTAAATGATACCAACATAAGTTTCACTCAGCGGTTAAGACATCTTTATTTTCTTCAAGAGGTCGTAGGTTTGAATAAAATAAAAAAATAATTAATTAAATGATAATTAATTAATCAAATCACATAGCATAACTCGTAATAACCTTATTTCTTAAAAACAGAAAGAAAAAAACTATTTTAACGTTAAAACTTATTATTTTTAGTTTAAATTACCACTCTCATTTTTATAGTTTGAGCTTAGTCTCAATTTAGTCCCTAAACTTAGTCTCAATTTGGCATTTATGGTTTAATTAAACCTCACAAATTTACCGTGAACAGTTTTGTACCCATCTAAAAACTAATTTTTTCATCATATTTTTTATTTTAGCGCTAAACCTTAATACAACCAACCAAATAAAAAGCACTAGCTCATTGCTAATGTTATGTAAATAAAAAAAAAAACACTACAAATATATGTATATATATATATTTTTTTTGAATGGCTACACACTGCATATTTAATATGGTAATAGAATGTAGAATTTGTATGGCTTGATCAATAAGTACAAACCAAATGAGAATTTTTAAGATTATTGAAACCAAATTAAAATTAAACTAAAATTATCGGAGGAAATGGGTAATTTAACCTAATTTTTAATTATTCTAACATCAATCTTTCCACATCCCTTATTTTTTTTATTATTTATTTTGTATTTAATTCTAATTCTAATTCATATACCAAATAAAAGTCAGATGACCAATTTTTAGGATTCAATTTCAACTACTCACCGACGTTCCTTTTATCCGTTGTCACGAGGTCTCGACCTTCCTCTTCCGGCTCCGCCTGCTCACCGTCGGCGTGCCCCATCTCATCTCCAGATTGCTGGAATCTCCGCCGCCGCGATGACCACCTACGGCACGATCCCCACCTCCGTCGCACCTGGAATCTCCAGCAACCTCCAGTACGTTTCCCGCGCCAAAGAGCGAGTTAAAGCCGGTCTCGCGACTCGCGCTCCATGGAAGCTCATGTTCGATTTCCACTCCTTCACCCTCCCTTTCAACTTCCGAGACACTTTCTCTCGCATCAGGACCAACATCGCCTACTTCCGTATGAATTATGTCATCATCGTCCTCATAATCCTGTTTTTAAGCCTCTTATGGCATCCGATTTCGCTCATAGTCTTTGCGGCCATGATCGCTGTTTGGTTGTTCCTGTATTTTCTTCGCGACGAGCCTCTGGCCGTCTTCGGCCGTTTGATTGATGATCGAGTGGTGTTGGCTGTGTTGTCGGTGCTGACGCTTGTGTTCTTGTTTCTGACGAGGGCTACGCTCAATATCCTGTTGTCGCTTCTGATCGGCGCCGTGGTGGTGTTGGTACACGCCGCCGTGAGGAAGACGGAGGACCTGTTTTTGGACGAAGAAGCTACCGCAGTCTTCACCGTCGGTCCGAATGCTCCTGGATCTTCAATTTCATTTTAA

mRNA sequence

ATGTGGTCAGGGCTGTGGCAGGGAGGCAGAGGGGGAGCGTGCGGGCAGAGGAATTCTTTGTCCAAAATGGACAGTTTATGGGATGTTGTGGTAGTGACAGCCATTTTCTTGACCACTATTATTGTCTACAGGAACTGCTTTAGACTTCTCAGATCAAACTTCTGCAGCCAACTTCCTCAGGGCACTCTCGGTTGGCCTTTCATTGGTGAGACCATTGAGTTCATCTCCTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGCCGCCGCCTGTATGGGAAGGTGTTCAAGTCTCATATTTTTGGAAGTCCTACCATTGTTTCTACAGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCCAAGGCTTTTGTACCGTCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAATGGGAGCTTGCATAAGAGAATCCATGGTCTTGTGGGAGCTTTCTTCAAGTCTCCACATCTCAAAGCTCAAATAACCAAGGATATGGAGAGTTATGTCAAACTATCAATGGCAAATTGGACAGAGAACAATCCTATTTACCTTCAAGATGAAACCAAAAATGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCCGGGCTTATGGCTTTCCCAGTGAACATACCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGTCGTAAAAGTAAAGAAGATAATACAAGAGAGAAAAAGCAGTGGCATTTCTCCTGTCCCAAAAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGAACAACTAACAGATAATCTTATAGCAGAGAATATGATTGACATGATGATTCCGGGTGAAGATTCAGTCCCAATTTTAATGACCCTTGCAGTAAACTACCTCTCCGATTGCCCGGCTGCACTTCAACATTTAACGGAGGAAAACTTAAGGATGAAAAGACTTAAAGAGCAGCTCAGAGAGTCGTTGCATTGGAGCGATTACTTATCGTTGCCATTTACGCAGAGTGTGATCACAGAGACTTTAAGGATGGGAAACGTCATAACCGGGGTGATGCGGAAAGCCATGAAAGACATGAAGATAAAAGGATATCTAATACCAAAAGGTTGGTCTGTTCTTACGTACTTTAGATCCGTTCATCTCGACGACAATCACTATGACTGGCCTTACCAATTCAATCCATGGAGATGGCAAGACAGAGACTTGAGCAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGGCTTGAAGCTTCCATCTTTCTTCATCACTTTGTCACTGAGTTCAGATGGATGGTTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGAATGAAGAAGAGGATGCCAGTTTGGGATTCAATTTCAACTACTCACCGACGTTCCTTTTATCCGTTGTCACGAGGTCTCGACCTTCCTCTTCCGGCTCCGCCTGCTCACCGTCGGCGTGCCCCATCTCATCTCCAGATTGCTGGAATCTCCGCCGCCGCGATGACCACCTACGGCACGATCCCCACCTCCGTCGCACCTGGAATCTCCAGCAACCTCCAGTACGTTTCCCGCGCCAAAGAGCGAGTTAAAGCCGGTCTCGCGACTCGCGCTCCATGGAAGCTCATGTTCGATTTCCACTCCTTCACCCTCCCTTTCAACTTCCGAGACACTTTCTCTCGCATCAGGACCAACATCGCCTACTTCCGTATGAATTATGTCATCATCGTCCTCATAATCCTGTTTTTAAGCCTCTTATGGCATCCGATTTCGCTCATAGTCTTTGCGGCCATGATCGCTGTTTGGTTGTTCCTGTATTTTCTTCGCGACGAGCCTCTGGCCGTCTTCGGCCGTTTGATTGATGATCGAGTGGTGTTGGCTGTGTTGTCGGTGCTGACGCTTGTGTTCTTGTTTCTGACGAGGGCTACGCTCAATATCCTGTTGTCGCTTCTGATCGGCGCCGTGGTGGTGTTGGTACACGCCGCCGTGAGGAAGACGGAGGACCTGTTTTTGGACGAAGAAGCTACCGCAGTCTTCACCGTCGGTCCGAATGCTCCTGGATCTTCAATTTCATTTTAA

Coding sequence (CDS)

ATGTGGTCAGGGCTGTGGCAGGGAGGCAGAGGGGGAGCGTGCGGGCAGAGGAATTCTTTGTCCAAAATGGACAGTTTATGGGATGTTGTGGTAGTGACAGCCATTTTCTTGACCACTATTATTGTCTACAGGAACTGCTTTAGACTTCTCAGATCAAACTTCTGCAGCCAACTTCCTCAGGGCACTCTCGGTTGGCCTTTCATTGGTGAGACCATTGAGTTCATCTCCTGTGCTTATTCCGACCGCCCTGAAACCTTCATGGACAAGCGCCGCCGCCTGTATGGGAAGGTGTTCAAGTCTCATATTTTTGGAAGTCCTACCATTGTTTCTACAGATGCAGAAGTCAGTAAGTTCATTCTGCAGAGTGATGCCAAGGCTTTTGTACCGTCTTACCCGAAATCTTTGACAGAATTGATGGGGAAATCTTCCATTTTGCTGATAAATGGGAGCTTGCATAAGAGAATCCATGGTCTTGTGGGAGCTTTCTTCAAGTCTCCACATCTCAAAGCTCAAATAACCAAGGATATGGAGAGTTATGTCAAACTATCAATGGCAAATTGGACAGAGAACAATCCTATTTACCTTCAAGATGAAACCAAAAATGATGGAGTTTCTCAAGCAACAGTTTCAAGAATTCATCGCCGGGCTTATGGCTTTCCCAGTGAACATACCCGGAAGCAGGCTTTACCGATCGTTGCAGGCAAGTTGGTCGTAAAAGTAAAGAAGATAATACAAGAGAGAAAAAGCAGTGGCATTTCTCCTGTCCCAAAAGATGTCATGGAAGTTTTGCTCAAAGATGAAAGTGAACAACTAACAGATAATCTTATAGCAGAGAATATGATTGACATGATGATTCCGGGTGAAGATTCAGTCCCAATTTTAATGACCCTTGCAGTAAACTACCTCTCCGATTGCCCGGCTGCACTTCAACATTTAACGGAGGAAAACTTAAGGATGAAAAGACTTAAAGAGCAGCTCAGAGAGTCGTTGCATTGGAGCGATTACTTATCGTTGCCATTTACGCAGAGTGTGATCACAGAGACTTTAAGGATGGGAAACGTCATAACCGGGGTGATGCGGAAAGCCATGAAAGACATGAAGATAAAAGGATATCTAATACCAAAAGGTTGGTCTGTTCTTACGTACTTTAGATCCGTTCATCTCGACGACAATCACTATGACTGGCCTTACCAATTCAATCCATGGAGATGGCAAGACAGAGACTTGAGCAGTTGTAACTTCACTCCTTTTGGAGGTGGACAAAGACTATGCCCTGGACTTGACTTGGCCAGGCTTGAAGCTTCCATCTTTCTTCATCACTTTGTCACTGAGTTCAGATGGATGGTTGAGGAAGATAGAATCATAAACTTCCCCACAGTGAGAATGAAGAAGAGGATGCCAGTTTGGGATTCAATTTCAACTACTCACCGACGTTCCTTTTATCCGTTGTCACGAGGTCTCGACCTTCCTCTTCCGGCTCCGCCTGCTCACCGTCGGCGTGCCCCATCTCATCTCCAGATTGCTGGAATCTCCGCCGCCGCGATGACCACCTACGGCACGATCCCCACCTCCGTCGCACCTGGAATCTCCAGCAACCTCCAGTACGTTTCCCGCGCCAAAGAGCGAGTTAAAGCCGGTCTCGCGACTCGCGCTCCATGGAAGCTCATGTTCGATTTCCACTCCTTCACCCTCCCTTTCAACTTCCGAGACACTTTCTCTCGCATCAGGACCAACATCGCCTACTTCCGTATGAATTATGTCATCATCGTCCTCATAATCCTGTTTTTAAGCCTCTTATGGCATCCGATTTCGCTCATAGTCTTTGCGGCCATGATCGCTGTTTGGTTGTTCCTGTATTTTCTTCGCGACGAGCCTCTGGCCGTCTTCGGCCGTTTGATTGATGATCGAGTGGTGTTGGCTGTGTTGTCGGTGCTGACGCTTGTGTTCTTGTTTCTGACGAGGGCTACGCTCAATATCCTGTTGTCGCTTCTGATCGGCGCCGTGGTGGTGTTGGTACACGCCGCCGTGAGGAAGACGGAGGACCTGTTTTTGGACGAAGAAGCTACCGCAGTCTTCACCGTCGGTCCGAATGCTCCTGGATCTTCAATTTCATTTTAA

Protein sequence

MWSGLWQGGRGGACGQRNSLSKMDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAGKLVVKVKKIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHRRSFYPLSRGLDLPLPAPPAHRRRAPSHLQIAGISAAAMTTYGTIPTSVAPGISSNLQYVSRAKERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILFLSLLWHPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRATLNILLSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSISF
Homology
BLAST of Sgr025287 vs. NCBI nr
Match: XP_038898401.1 (LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida])

HSP 1 Score: 995.3 bits (2572), Expect = 2.6e-286
Identity = 531/703 (75.53%), Postives = 580/703 (82.50%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAIF TTII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFM+KRRRLYGKVFKSHIFGSPTIVSTD+EVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMNKRRRLYGKVFKSHIFGSPTIVSTDSEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YV+LSMANWTENN IYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVQLSMANWTENNLIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QILVKILISLNPGQRMEFLKKQFQDFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GISPVPKDVMEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISPVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK LKEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGLKEQLGETLHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PY+FNPWRWQDRDLSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDRDLSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHRRSFY 502
           RLCPGLDLARLEASIFLHHFVT FR+ +  D   + P+VR+        + S  H     
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRL--------TSSNFH----- 480

Query: 503 PLSRGLDLPLPAPPAHRRRAPSHLQIAGISAAAMTTYGTIPTSVAPGISSNLQYVSRAKE 562
                 +LP P  P+     P      G S A MTTYGTIPTS APG SS+L +VSR K+
Sbjct: 481 ------NLP-PLSPSAFPNXP------GFSPAPMTTYGTIPTSAAPGTSSDLDFVSRGKQ 540

Query: 563 RVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILFLSLLWH 622
           R+KAGLATR PW+LMFDFHSFTLPFNF +TFSRI+TNI YFRMNYVIIVL ILFLSLLWH
Sbjct: 541 RLKAGLATRVPWRLMFDFHSFTLPFNFHETFSRIKTNIVYFRMNYVIIVLSILFLSLLWH 600

Query: 623 PISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRATLNILL 682
           PISLIVF AM+AVWLFLYFLRDEPL +FGRLI+DR+V+A+LSV TLVFLFLT+ATLNILL
Sbjct: 601 PISLIVFTAMLAVWLFLYFLRDEPLILFGRLINDRLVMALLSVFTLVFLFLTKATLNILL 660

Query: 683 SLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSIS 705
           SLLI AV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+S
Sbjct: 661 SLLIAAVLVLIHAAMRKTDDLFLDEGATTVYTFGSDAPGTSVS 675

BLAST of Sgr025287 vs. NCBI nr
Match: KAE8646515.1 (hypothetical protein Csa_016429 [Cucumis sativus])

HSP 1 Score: 988.4 bits (2554), Expect = 3.2e-284
Identity = 527/706 (74.65%), Postives = 577/706 (81.73%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAIF TTII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMANWTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GIS VPKD+MEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PY+FNPWRWQDR++SSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHRRSFY 502
           RLCPGLDLARLEASIFLHHFVT FR                K    VW   + +  + F 
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFR----------------KSDFSVWFGFNYSPTKHFI 480

Query: 503 PLSRGLDLPLPAPPAHRRRAPSHL-QIAGISAAA-MTTYGTIPTSVAPGISSNLQYVSRA 562
              R  D  LP        +P+ L +I GISA+A MT+YGTIPTS APG SS+L +VSR 
Sbjct: 481 ---RRSDSQLPPLSLSPSSSPNLLFEIDGISASAPMTSYGTIPTSTAPGTSSDLDFVSRG 540

Query: 563 KERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILFLSLL 622
           K+R+KAGLATR PW+LMFDFHSFTLP NF +TFSRI+TNI YFRMNYVIIVL+ILF SL+
Sbjct: 541 KQRLKAGLATRVPWRLMFDFHSFTLPLNFHETFSRIKTNIVYFRMNYVIIVLLILFSSLI 600

Query: 623 WHPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRATLNI 682
           WHPISLIV  AM+AVWLFLYFLRDEPL + GRLI+ R+V+AVLSV TLVFLFLT+ATLNI
Sbjct: 601 WHPISLIVLTAMLAVWLFLYFLRDEPLILLGRLINYRLVMAVLSVFTLVFLFLTKATLNI 660

Query: 683 LLSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSISF 706
           LLSLLIGAV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+SF
Sbjct: 661 LLSLLIGAVLVLIHAALRKTDDLFLDEGATTVYTFGSDAPGTSVSF 686

BLAST of Sgr025287 vs. NCBI nr
Match: XP_011659645.1 (LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis sativus])

HSP 1 Score: 988.0 bits (2553), Expect = 4.1e-284
Identity = 525/705 (74.47%), Postives = 577/705 (81.84%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAIF TTII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMANWTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GIS VPKD+MEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PY+FNPWRWQDR++SSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHRRSFY 502
           RLCPGLDLARLEASIFLHHFVT FR+ +  D   + P+VR     P+  S S++    F 
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTDEAFH-PSVRFTXLPPLSLSPSSSPNLLF- 480

Query: 503 PLSRGLDLPLPAPPAHRRRAPSHLQIAGISAAA-MTTYGTIPTSVAPGISSNLQYVSRAK 562
                                   +I GISA+A MT+YGTIPTS APG SS+L +VSR K
Sbjct: 481 ------------------------EIDGISASAPMTSYGTIPTSTAPGTSSDLDFVSRGK 540

Query: 563 ERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILFLSLLW 622
           +R+KAGLATR PW+LMFDFHSFTLP NF +TFSRI+TNI YFRMNYVIIVL+ILF SL+W
Sbjct: 541 QRLKAGLATRVPWRLMFDFHSFTLPLNFHETFSRIKTNIVYFRMNYVIIVLLILFSSLIW 600

Query: 623 HPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRATLNIL 682
           HPISLIV  AM+AVWLFLYFLRDEPL + GRLI+ R+V+AVLSV TLVFLFLT+ATLNIL
Sbjct: 601 HPISLIVLTAMLAVWLFLYFLRDEPLILLGRLINYRLVMAVLSVFTLVFLFLTKATLNIL 660

Query: 683 LSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSISF 706
           LSLLIGAV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+SF
Sbjct: 661 LSLLIGAVLVLIHAALRKTDDLFLDEGATTVYTFGSDAPGTSVSF 678

BLAST of Sgr025287 vs. NCBI nr
Match: KAA0059438.1 (cytochrome p450 [Cucumis melo var. makuwa])

HSP 1 Score: 985.3 bits (2546), Expect = 2.7e-283
Identity = 528/709 (74.47%), Postives = 580/709 (81.81%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAI   TII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMA+WTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG-------------------------KLV 262
              Q  V  +     G   E  +KQ    +AG                          +V
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASWTAKRNMV 240

Query: 263 VKVKKIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTL 322
             VKKIIQERKS GIS VPKDVMEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTL
Sbjct: 241 KLVKKIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTL 300

Query: 323 AVNYLSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITG 382
           AV YLSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I G
Sbjct: 301 AVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIG 360

Query: 383 VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPF 442
           VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PY+FNPWRWQ+R+LSSCNFTPF
Sbjct: 361 VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPF 420

Query: 443 GGGQRLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHR 502
           GGGQRLCPGLDLARLEASIFLHHFVT FR+ +  D                         
Sbjct: 421 GGGQRLCPGLDLARLEASIFLHHFVTHFRFQLLTD------------------------- 480

Query: 503 RSFYPLSRGLDLPLPAPPAHRRRAPSHLQIA-GISAAA-MTTYGTIPTSVAPGISSNLQY 562
            +F+P  R  + P  +P +    +P+ L I  GISAAA MT+YGTIPTS APG SS+L +
Sbjct: 481 EAFHPSVRFTNFPPLSPSS----SPNLLFIIDGISAAASMTSYGTIPTSTAPGTSSDLDF 540

Query: 563 VSRAKERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILF 622
           VSR K+R+KAGLATR PW+LMFDFHSFTLPFNF +TFSRI+TNI YFRMNYVIIVL+ILF
Sbjct: 541 VSRGKQRLKAGLATRVPWRLMFDFHSFTLPFNFHETFSRIKTNIVYFRMNYVIIVLLILF 600

Query: 623 LSLLWHPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRA 682
            SL+WHPISLIVF AM+AVWLFLYFLRDEPL +FGRLI+DR+V+AVLSV TLVFLFLT+A
Sbjct: 601 FSLIWHPISLIVFTAMLAVWLFLYFLRDEPLILFGRLINDRLVMAVLSVFTLVFLFLTKA 660

Query: 683 TLNILLSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSIS 705
           TLNILLSLLIGAV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+S
Sbjct: 661 TLNILLSLLIGAVLVLIHAALRKTDDLFLDEGATTVYTFGSDAPGTSVS 676

BLAST of Sgr025287 vs. NCBI nr
Match: ADN34104.1 (cytochrome p450 [Cucumis melo subsp. melo])

HSP 1 Score: 983.0 bits (2540), Expect = 1.3e-282
Identity = 527/705 (74.75%), Postives = 579/705 (82.13%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAI   TII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMA+WTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GIS VPKDVMEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLI KGWSVLTYFRSVHLDDNHYD PY+FNPWRWQ+R+LSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHRRSFY 502
           RLCPGLDLARLEASIFLHHFVT FR+ +  D                          +F+
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTD-------------------------EAFH 480

Query: 503 PLSRGLDLPLPAPPAHRRRAPSHLQIA-GISAAA-MTTYGTIPTSVAPGISSNLQYVSRA 562
           P  R  + P  +P +    +P+ L I  GISAAA MT+YGTIPTS APG SS+L +VSR 
Sbjct: 481 PSVRFTNFPPLSPSS----SPNLLFIIDGISAAASMTSYGTIPTSTAPGTSSDLDFVSRG 540

Query: 563 KERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILFLSLL 622
           K+R+KAGLATR PW+LMFDFHSFTLPFNF +TFSRI+TNI YFRMNYVIIVL+ILF SL+
Sbjct: 541 KQRLKAGLATRVPWRLMFDFHSFTLPFNFHETFSRIKTNIVYFRMNYVIIVLLILFFSLI 600

Query: 623 WHPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRATLNI 682
           WHPISLIVF AM+AVWLFLYFLRDEPL +FGRLI+DR+V+AVLSV TLVFLFLT+ATLNI
Sbjct: 601 WHPISLIVFTAMLAVWLFLYFLRDEPLILFGRLINDRLVMAVLSVFTLVFLFLTKATLNI 660

Query: 683 LLSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSIS 705
           LLSLLIGAV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+S
Sbjct: 661 LLSLLIGAVLVLIHAALRKTDDLFLDEGATTVYTFGSDAPGTSVS 672

BLAST of Sgr025287 vs. ExPASy Swiss-Prot
Match: Q94IA6 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=3702 GN=CYP90D1 PE=1 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.6e-148
Identity = 264/438 (60.27%), Postives = 328/438 (74.89%), Query Frame = 0

Query: 57  QLPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 116
           + P G+LGWP IGETIEF+S AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 117 KFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDM 176
           + +LQSD+ AFVP YPK++ ELMGKSSILLINGSLH+R HGLVG+F KSP LKAQI +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 177 ESYVKLSMANWTENNPIYLQDETKNDG---VSQATVS-----RIHRRAYGFPSEHTRKQA 236
             ++  SM  W+E+ P+ LQD +K      +++A +S      +      F +  +   +
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 237 LPI------------VAGKLVVKVKKIIQ-----ERKSSGISPVPKDVMEVLLKDESEQL 296
           LPI                +V +V++II+      +       + KDV++VLLKD SE L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 297 TDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLH 356
           T NLIA NMIDMMIPG DSVP+L+TLAV +LSD PAAL  LTEEN+++K LKE   E L+
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 357 WSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 416
           W+DYLSLPFTQ VITETLRMGNVI GVMRKAMKD++IKGY+IPKGW  L Y RSVHLD  
Sbjct: 350 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 409

Query: 417 HYDWPYQFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRWMVEE 470
           +Y+ PY+FNPWRWQ+RD+++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT FRW+ EE
Sbjct: 410 YYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEE 469

BLAST of Sgr025287 vs. ExPASy Swiss-Prot
Match: Q94IW5 (Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 5.3e-141
Identity = 242/437 (55.38%), Postives = 333/437 (76.20%), Query Frame = 0

Query: 56  SQLPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGK-VFKSHIFGSPTIVSTDAE 115
           ++LP G+ GWP +GET+EF+SCAYS RPE F+DKRR+L+G  VF+SH+FGS T+V+ DAE
Sbjct: 47  ARLPPGSFGWPVVGETLEFVSCAYSPRPEAFVDKRRKLHGSAVFRSHLFGSATVVTADAE 106

Query: 116 VSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITK 175
           VS+F+LQSDA+AFVP YP+SLTELMGKSSILLING+L +R+HGLVGAFFKS HLK+Q+T 
Sbjct: 107 VSRFVLQSDARAFVPWYPRSLTELMGKSSILLINGALQRRVHGLVGAFFKSSHLKSQLTA 166

Query: 176 DMESYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAG 235
           DM   +  +++++ +++ +++Q   K+  V +  V  +     G   +  ++Q    + G
Sbjct: 167 DMRRRLSPALSSFPDSSLLHVQHLAKS-VVFEILVRGLIGLEAGEEMQQLKQQFQEFIVG 226

Query: 236 ---------------------KLVVKVKKIIQERKS--SGISPVPKDVMEVLLKDESEQL 295
                                K+   +++II+E+++  +  SP P+D ++VL+ D S++L
Sbjct: 227 LMSLPIKLPGTRLYRSLQAKKKMARLIQRIIREKRARRAAASP-PRDAIDVLIGDGSDEL 286

Query: 296 TDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLH 355
           TD LI++NMID+MIP EDSVP+L+TLAV +LS+CP AL  L EEN+++KR K  + E+L 
Sbjct: 287 TDELISDNMIDLMIPAEDSVPVLITLAVKFLSECPLALHQLEEENIQLKRRKTDMGETLQ 346

Query: 356 WSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 415
           W+DY+SL FTQ VITETLR+GN+I G+MRKA++D+++KG+LIPKGW V  YFRSVHLDD 
Sbjct: 347 WTDYMSLSFTQHVITETLRLGNIIGGIMRKAVRDVEVKGHLIPKGWCVFVYFRSVHLDDT 406

Query: 416 HYDWPYQFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRWMVEE 469
            YD PY+FNPWRW+++D+S+ +FTPFGGGQRLCPGLDLARLEASIFLHH VT FRW+ EE
Sbjct: 407 LYDEPYKFNPWRWKEKDMSNGSFTPFGGGQRLCPGLDLARLEASIFLHHLVTSFRWVAEE 466

BLAST of Sgr025287 vs. ExPASy Swiss-Prot
Match: Q9M066 (3-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=3702 GN=CYP90C1 PE=1 SV=3)

HSP 1 Score: 439.5 bits (1129), Expect = 7.2e-122
Identity = 222/441 (50.34%), Postives = 304/441 (68.93%), Query Frame = 0

Query: 58  LPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 117
           +P G+LGWP IGET+ FI+C YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 118 FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDME 177
            +LQ+    FVP+YPKS+TEL+G++SIL ING   KR+H L+GAF +SPHLK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 178 SYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAG--- 237
           + V L++A+W +   +++QDE K     +  V  +   + G      + +    + G   
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKK-MTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLIC 248

Query: 238 ------------------KLVVKVKKIIQERKSSGISPVP-KDVMEVLLKD----ESEQL 297
                             +L+  VKK+++ER+ +  +  P  DV++VLL+D    E +  
Sbjct: 249 IPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQ 308

Query: 298 TDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLH 357
             + ++  +++MMIPGE+++P  MTLAV +LSD P AL  L EEN+ MKR K +L E   
Sbjct: 309 PSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYK 368

Query: 358 WSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 417
           W+DY+SL FTQ+VI ETLRM N+I GV RKA+KD++IKGYLIPKGW VL  F SVH+D++
Sbjct: 369 WTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDED 428

Query: 418 HYDWPYQFNPWRWQDRDLSSCN----FTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRW 469
            YD PYQF+PWRW DR   S N    FTPFGGGQRLCPGL+L++LE SIFLHH VT + W
Sbjct: 429 IYDNPYQFDPWRW-DRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSW 488

BLAST of Sgr025287 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.5e-87
Identity = 177/439 (40.32%), Postives = 265/439 (60.36%), Query Frame = 0

Query: 58  LPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 117
           LP G+LG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 118 FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDME 177
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSLHKR+H L  +F  S  +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 178 SYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAGKLV 237
             V+ ++ +W+    + L +E K     + TV ++     G  SE  RK+ L ++ G   
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKI-TFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 210

Query: 238 VKV-------KKIIQERK--SSGISPV--------------PKDVMEVLLKDESEQLTDN 297
           + +       +K IQ R+  +  ++ V               KD++  LL  + +  +D 
Sbjct: 211 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDE 270

Query: 298 LIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLHWSD 357
            I + ++ +++ G ++   +MTLAV +L++ P AL  L EE+ +++ +K     SL WSD
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSD 330

Query: 358 YLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD 417
           Y S+PFTQ V+ ETLR+ N+I GV R+AM D++IKGY IPKGW V + FR+VHLD NH+ 
Sbjct: 331 YKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFK 390

Query: 418 WPYQFNPWRWQDRDLS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRWM-VE 470
               FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+  E
Sbjct: 391 DARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAE 450

BLAST of Sgr025287 vs. ExPASy Swiss-Prot
Match: Q5CCK1 (Cytochrome P450 90A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A4 PE=2 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 1.7e-75
Identity = 168/454 (37.00%), Postives = 245/454 (53.96%), Query Frame = 0

Query: 56  SQLPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEV 115
           +++P G+ G P IGET+  IS   +  PE F+D+R   +G VF +H+FG  T+ S D   
Sbjct: 40  ARMPPGSTGLPLIGETLRLISAYKTPNPEPFIDERVARHGGVFTTHVFGERTVFSADPAF 99

Query: 116 SKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKD 175
           ++ +L ++ +A   SYP S+  L+G  S+LL  G+ HKR+H L       P     +   
Sbjct: 100 NRLLLAAEGRAVHSSYPSSIATLLGARSLLLTRGAAHKRLHSLTLTRLGRP-ASPPLLAH 159

Query: 176 MESYVKLSMANWTENNPIYLQDETKN----------DGVSQATVSRIHRRAY-------- 235
           ++  V  +M  W     + L DE K             +     +   RR Y        
Sbjct: 160 IDRLVLATMRQWEPAATVRLMDEAKKITFNLTVKQLVSIEPGPWTESLRREYVKLIDGFF 219

Query: 236 --GFPSEH-----TRKQALPI---VAGKLVVKVKKIIQERKSSGIS-------PVPKDVM 295
              FP  +     T  QAL     VAG L   +KK ++E+  +G S          KD++
Sbjct: 220 SIPFPLAYFLPFTTYGQALKARKKVAGALREVIKKRMEEKAENGGSIGDDEGKKEKKDMV 279

Query: 296 EVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMK 355
           E LL+ E    ++  + +  + +++ G ++  +LMTLAV +L++ PAAL  L EE+  ++
Sbjct: 280 EELLQAEGGSFSEEEMVDFCLSLLVAGYETTSVLMTLAVKFLTETPAALAELKEEHANIR 339

Query: 356 RLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVL 415
            +K +  + L WSDY S+PFTQ VI ETLR+GN+I+GV R+A  D+  K Y IPKG  + 
Sbjct: 340 DMKGK-NQPLEWSDYKSMPFTQCVINETLRVGNIISGVFRRANTDIHYKDYTIPKGCKIF 399

Query: 416 TYFRSVHLDDNHYDWPYQFNPWRWQ-----DRDLSSCNFTPFGGGQRLCPGLDLARLEAS 469
             FR+VHL++ HY+    FNPWRWQ        + +  FTPFGGG RLCPG +LAR+  S
Sbjct: 400 ASFRAVHLNNEHYENARTFNPWRWQINNKLQNAVGANIFTPFGGGPRLCPGYELARVVVS 459

BLAST of Sgr025287 vs. ExPASy TrEMBL
Match: A0A5A7UW93 (Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G001660 PE=3 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 1.3e-283
Identity = 528/709 (74.47%), Postives = 580/709 (81.81%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAI   TII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMA+WTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG-------------------------KLV 262
              Q  V  +     G   E  +KQ    +AG                          +V
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQASWTAKRNMV 240

Query: 263 VKVKKIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTL 322
             VKKIIQERKS GIS VPKDVMEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTL
Sbjct: 241 KLVKKIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTL 300

Query: 323 AVNYLSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITG 382
           AV YLSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I G
Sbjct: 301 AVKYLSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIG 360

Query: 383 VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPF 442
           VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PY+FNPWRWQ+R+LSSCNFTPF
Sbjct: 361 VMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPF 420

Query: 443 GGGQRLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHR 502
           GGGQRLCPGLDLARLEASIFLHHFVT FR+ +  D                         
Sbjct: 421 GGGQRLCPGLDLARLEASIFLHHFVTHFRFQLLTD------------------------- 480

Query: 503 RSFYPLSRGLDLPLPAPPAHRRRAPSHLQIA-GISAAA-MTTYGTIPTSVAPGISSNLQY 562
            +F+P  R  + P  +P +    +P+ L I  GISAAA MT+YGTIPTS APG SS+L +
Sbjct: 481 EAFHPSVRFTNFPPLSPSS----SPNLLFIIDGISAAASMTSYGTIPTSTAPGTSSDLDF 540

Query: 563 VSRAKERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILF 622
           VSR K+R+KAGLATR PW+LMFDFHSFTLPFNF +TFSRI+TNI YFRMNYVIIVL+ILF
Sbjct: 541 VSRGKQRLKAGLATRVPWRLMFDFHSFTLPFNFHETFSRIKTNIVYFRMNYVIIVLLILF 600

Query: 623 LSLLWHPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRA 682
            SL+WHPISLIVF AM+AVWLFLYFLRDEPL +FGRLI+DR+V+AVLSV TLVFLFLT+A
Sbjct: 601 FSLIWHPISLIVFTAMLAVWLFLYFLRDEPLILFGRLINDRLVMAVLSVFTLVFLFLTKA 660

Query: 683 TLNILLSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSIS 705
           TLNILLSLLIGAV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+S
Sbjct: 661 TLNILLSLLIGAVLVLIHAALRKTDDLFLDEGATTVYTFGSDAPGTSVS 676

BLAST of Sgr025287 vs. ExPASy TrEMBL
Match: E5GCA5 (Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 983.0 bits (2540), Expect = 6.4e-283
Identity = 527/705 (74.75%), Postives = 579/705 (82.13%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAI   TII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMA+WTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GIS VPKDVMEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLI KGWSVLTYFRSVHLDDNHYD PY+FNPWRWQ+R+LSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVWDSISTTHRRSFY 502
           RLCPGLDLARLEASIFLHHFVT FR+ +  D                          +F+
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRFQLLTD-------------------------EAFH 480

Query: 503 PLSRGLDLPLPAPPAHRRRAPSHLQIA-GISAAA-MTTYGTIPTSVAPGISSNLQYVSRA 562
           P  R  + P  +P +    +P+ L I  GISAAA MT+YGTIPTS APG SS+L +VSR 
Sbjct: 481 PSVRFTNFPPLSPSS----SPNLLFIIDGISAAASMTSYGTIPTSTAPGTSSDLDFVSRG 540

Query: 563 KERVKAGLATRAPWKLMFDFHSFTLPFNFRDTFSRIRTNIAYFRMNYVIIVLIILFLSLL 622
           K+R+KAGLATR PW+LMFDFHSFTLPFNF +TFSRI+TNI YFRMNYVIIVL+ILF SL+
Sbjct: 541 KQRLKAGLATRVPWRLMFDFHSFTLPFNFHETFSRIKTNIVYFRMNYVIIVLLILFFSLI 600

Query: 623 WHPISLIVFAAMIAVWLFLYFLRDEPLAVFGRLIDDRVVLAVLSVLTLVFLFLTRATLNI 682
           WHPISLIVF AM+AVWLFLYFLRDEPL +FGRLI+DR+V+AVLSV TLVFLFLT+ATLNI
Sbjct: 601 WHPISLIVFTAMLAVWLFLYFLRDEPLILFGRLINDRLVMAVLSVFTLVFLFLTKATLNI 660

Query: 683 LLSLLIGAVVVLVHAAVRKTEDLFLDEEATAVFTVGPNAPGSSIS 705
           LLSLLIGAV+VL+HAA+RKT+DLFLDE AT V+T G +APG+S+S
Sbjct: 661 LLSLLIGAVLVLIHAALRKTDDLFLDEGATTVYTFGSDAPGTSVS 672

BLAST of Sgr025287 vs. ExPASy TrEMBL
Match: A0A0A0K7W8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1)

HSP 1 Score: 760.0 bits (1961), Expect = 8.8e-216
Identity = 383/468 (81.84%), Postives = 404/468 (86.32%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAIF TTII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFIS AYSDR
Sbjct: 1   MDSLWDVLVVTAIFFTTIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISSAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMANWTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMANWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GIS VPKD+MEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISTVPKDIMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD PY+FNPWRWQDR++SSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQDREMSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVW 470
           RLCPGLDLARLEASIFLHHFVT FRW+ EED+IINFPTVRMKKRMPVW
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVW 467

BLAST of Sgr025287 vs. ExPASy TrEMBL
Match: A0A1S3CGU6 (3-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC103500734 PE=3 SV=1)

HSP 1 Score: 748.4 bits (1931), Expect = 2.7e-212
Identity = 381/468 (81.41%), Postives = 402/468 (85.90%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MDSLWDV+VVTAI   TII+YRNCFRLLRS FC+QLP G+LGWPFIGETIEFISCAYSDR
Sbjct: 1   MDSLWDVLVVTAI---TIILYRNCFRLLRSKFCNQLPLGSLGWPFIGETIEFISCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SILLINGSL KRIHGLVGAFFKS HLKAQITKDME YVKLSMA+WTENNPIYLQDETKN 
Sbjct: 121 SILLINGSLQKRIHGLVGAFFKSSHLKAQITKDMECYVKLSMASWTENNPIYLQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    +AG                      +V  VK
Sbjct: 181 SF-QVLVKILISLNPGQSMEFLKKQFQEFIAGLMALPVNIPGSRLYQSLQAKRNMVKLVK 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
           KIIQERKS GIS VPKDVMEVLLKDE+E+LTD+LIAENMIDMMIPGEDSVPILMTLAV Y
Sbjct: 241 KIIQERKSCGISTVPKDVMEVLLKDENEELTDDLIAENMIDMMIPGEDSVPILMTLAVKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCPAALQ LTEENL+MK  KEQL E+LHWSDYLS+PFTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPAALQQLTEENLKMKGQKEQLGEALHWSDYLSMPFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKDMKIKGYLI KGWSVLTYFRSVHLDDNHYD PY+FNPWRWQ+R+LSSCNFTPFGGGQ
Sbjct: 361 AMKDMKIKGYLILKGWSVLTYFRSVHLDDNHYDCPYKFNPWRWQERELSSCNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVW 470
           RLCPGLDLARLEASIFLHHFVT FRW+ EED+IINFPTVRMKKRMPVW
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTHFRWIAEEDKIINFPTVRMKKRMPVW 464

BLAST of Sgr025287 vs. ExPASy TrEMBL
Match: A0A7N2LKP8 (Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 1.6e-188
Identity = 335/468 (71.58%), Postives = 381/468 (81.41%), Query Frame = 0

Query: 23  MDSLWDVVVVTAIFLTTIIVYRNCFRLLRSNFCSQLPQGTLGWPFIGETIEFISCAYSDR 82
           MD++W V++VT IFL+TII+Y+N FR LRS   S LP GTLG PFIGETIEF+SCAYSDR
Sbjct: 1   MDNMW-VLLVTTIFLSTIILYKNRFR-LRSKHRSPLPLGTLGLPFIGETIEFVSCAYSDR 60

Query: 83  PETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 142
           PE+FMDKRR +YGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS
Sbjct: 61  PESFMDKRRSMYGKVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKS 120

Query: 143 SILLINGSLHKRIHGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKND 202
           SIL+INGSL KRIHGL+GAFFKSPHLKAQIT+DM  YV+ SMANW +++PIY+QDETKN 
Sbjct: 121 SILVINGSLQKRIHGLIGAFFKSPHLKAQITRDMHKYVQESMANWKDDHPIYMQDETKNI 180

Query: 203 GVSQATVSRIHRRAYGFPSEHTRKQALPIVAG---------------------KLVVKVK 262
              Q  V  +     G   E  +KQ    ++G                     K+V  V+
Sbjct: 181 AF-QVLVKALISLDPGEEMEFLKKQFQEFISGLMSLPINIPGTRLYRSLQAKKKMVKLVQ 240

Query: 263 KIIQERKSSGISPVPKDVMEVLLKDESEQLTDNLIAENMIDMMIPGEDSVPILMTLAVNY 322
            +I  RK SG S VPKDV++VLL D SEQLT++LIA+NMIDMMIPGEDSVP+LMTLA+ Y
Sbjct: 241 NVILARKHSGFSNVPKDVVDVLLNDSSEQLTEDLIADNMIDMMIPGEDSVPVLMTLAIKY 300

Query: 323 LSDCPAALQHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRK 382
           LSDCP ALQ LTEENL++K LK QL + L+WSDYLSL FTQSVITETLRMGN+I GVMRK
Sbjct: 301 LSDCPTALQQLTEENLKLKELKAQLGQPLYWSDYLSLSFTQSVITETLRMGNIIIGVMRK 360

Query: 383 AMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFTPFGGGQ 442
           AMKD++IKGYLIPKGW V  YFRSVHLD NHYDWPY+FNPWRWQD+D+SS NFTPFGGGQ
Sbjct: 361 AMKDIEIKGYLIPKGWCVFAYFRSVHLDANHYDWPYKFNPWRWQDKDMSSSNFTPFGGGQ 420

Query: 443 RLCPGLDLARLEASIFLHHFVTEFRWMVEEDRIINFPTVRMKKRMPVW 470
           RLCPGLDLARLEASIFLHHFVT+F W  EED I+NFPTVRMK+RMP+W
Sbjct: 421 RLCPGLDLARLEASIFLHHFVTQFSWRAEEDTIVNFPTVRMKRRMPIW 465

BLAST of Sgr025287 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 527.3 bits (1357), Expect = 1.9e-149
Identity = 264/438 (60.27%), Postives = 328/438 (74.89%), Query Frame = 0

Query: 57  QLPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVS 116
           + P G+LGWP IGETIEF+S AYSDRPE+FMDKRR +YG+VFKSHIFG+ TIVSTDAEV+
Sbjct: 50  KFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEVN 109

Query: 117 KFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDM 176
           + +LQSD+ AFVP YPK++ ELMGKSSILLINGSLH+R HGLVG+F KSP LKAQI +DM
Sbjct: 110 RAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRDM 169

Query: 177 ESYVKLSMANWTENNPIYLQDETKNDG---VSQATVS-----RIHRRAYGFPSEHTRKQA 236
             ++  SM  W+E+ P+ LQD +K      +++A +S      +      F +  +   +
Sbjct: 170 HKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDLEELKREFENFISGLMS 229

Query: 237 LPI------------VAGKLVVKVKKIIQ-----ERKSSGISPVPKDVMEVLLKDESEQL 296
           LPI                +V +V++II+      +       + KDV++VLLKD SE L
Sbjct: 230 LPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSEHL 289

Query: 297 TDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLH 356
           T NLIA NMIDMMIPG DSVP+L+TLAV +LSD PAAL  LTEEN+++K LKE   E L+
Sbjct: 290 THNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKELTGEPLY 349

Query: 357 WSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 416
           W+DYLSLPFTQ VITETLRMGNVI GVMRKAMKD++IKGY+IPKGW  L Y RSVHLD  
Sbjct: 350 WNDYLSLPFTQKVITETLRMGNVIIGVMRKAMKDVEIKGYVIPKGWCFLAYLRSVHLDKL 409

Query: 417 HYDWPYQFNPWRWQDRDLSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRWMVEE 470
           +Y+ PY+FNPWRWQ+RD+++ +F+PFGGGQRLCPGLDLARLE S+FLHH VT FRW+ EE
Sbjct: 410 YYESPYKFNPWRWQERDMNTSSFSPFGGGQRLCPGLDLARLETSVFLHHLVTRFRWIAEE 469

BLAST of Sgr025287 vs. TAIR 10
Match: AT4G36380.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 439.5 bits (1129), Expect = 5.1e-123
Identity = 222/441 (50.34%), Postives = 304/441 (68.93%), Query Frame = 0

Query: 58  LPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 117
           +P G+LGWP IGET+ FI+C YS RP TFMDKR+ LYGKVFK++I G+P I+STDAEV+K
Sbjct: 69  IPNGSLGWPVIGETLNFIACGYSSRPVTFMDKRKSLYGKVFKTNIIGTPIIISTDAEVNK 128

Query: 118 FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDME 177
            +LQ+    FVP+YPKS+TEL+G++SIL ING   KR+H L+GAF +SPHLK +IT+D+E
Sbjct: 129 VVLQNHGNTFVPAYPKSITELLGENSILSINGPHQKRLHTLIGAFLRSPHLKDRITRDIE 188

Query: 178 SYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAG--- 237
           + V L++A+W +   +++QDE K     +  V  +   + G      + +    + G   
Sbjct: 189 ASVVLTLASWAQLPLVHVQDEIKK-MTFEILVKVLMSTSPGEDMNILKLEFEEFIKGLIC 248

Query: 238 ------------------KLVVKVKKIIQERKSSGISPVP-KDVMEVLLKD----ESEQL 297
                             +L+  VKK+++ER+ +  +  P  DV++VLL+D    E +  
Sbjct: 249 IPIKFPGTRLYKSLKAKERLIKMVKKVVEERQVAMTTTSPANDVVDVLLRDGGDSEKQSQ 308

Query: 298 TDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLH 357
             + ++  +++MMIPGE+++P  MTLAV +LSD P AL  L EEN+ MKR K +L E   
Sbjct: 309 PSDFVSGKIVEMMIPGEETMPTAMTLAVKFLSDNPVALAKLVEENMEMKRRKLELGEEYK 368

Query: 358 WSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDN 417
           W+DY+SL FTQ+VI ETLRM N+I GV RKA+KD++IKGYLIPKGW VL  F SVH+D++
Sbjct: 369 WTDYMSLSFTQNVINETLRMANIINGVWRKALKDVEIKGYLIPKGWCVLASFISVHMDED 428

Query: 418 HYDWPYQFNPWRWQDRDLSSCN----FTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRW 469
            YD PYQF+PWRW DR   S N    FTPFGGGQRLCPGL+L++LE SIFLHH VT + W
Sbjct: 429 IYDNPYQFDPWRW-DRINGSANSSICFTPFGGGQRLCPGLELSKLEISIFLHHLVTRYSW 488

BLAST of Sgr025287 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 325.5 bits (833), Expect = 1.1e-88
Identity = 177/439 (40.32%), Postives = 265/439 (60.36%), Query Frame = 0

Query: 58  LPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 117
           LP G+LG P IGET + I    ++ PE F+D+R   YG VF +H+FG PTI S D E ++
Sbjct: 31  LPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLFGEPTIFSADPETNR 90

Query: 118 FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDME 177
           F+LQ++ K F  SYP S+  L+GK S+LL+ GSLHKR+H L  +F  S  +K  +  D++
Sbjct: 91  FVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFANSSIIKDHLMLDID 150

Query: 178 SYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAGKLV 237
             V+ ++ +W+    + L +E K     + TV ++     G  SE  RK+ L ++ G   
Sbjct: 151 RLVRFNLDSWSSR--VLLMEEAKKI-TFELTVKQLMSFDPGEWSESLRKEYLLVIEGFFS 210

Query: 238 VKV-------KKIIQERK--SSGISPV--------------PKDVMEVLLKDESEQLTDN 297
           + +       +K IQ R+  +  ++ V               KD++  LL  + +  +D 
Sbjct: 211 LPLPLFSTTYRKAIQARRKVAEALTVVVMKRREEEEEGAERKKDMLAALLAAD-DGFSDE 270

Query: 298 LIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAALQHLTEENLRMKRLKEQLRESLHWSD 357
            I + ++ +++ G ++   +MTLAV +L++ P AL  L EE+ +++ +K     SL WSD
Sbjct: 271 EIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEKIRAMKSD-SYSLEWSD 330

Query: 358 YLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYLIPKGWSVLTYFRSVHLDDNHYD 417
           Y S+PFTQ V+ ETLR+ N+I GV R+AM D++IKGY IPKGW V + FR+VHLD NH+ 
Sbjct: 331 YKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKGWKVFSSFRAVHLDPNHFK 390

Query: 418 WPYQFNPWRWQDRDLS---SCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTEFRWM-VE 470
               FNPWRWQ   ++   S  FTPFGGG RLCPG +LAR+  S+FLH  VT F W+  E
Sbjct: 391 DARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVALSVFLHRLVTGFSWVPAE 450

BLAST of Sgr025287 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 273.5 bits (698), Expect = 4.8e-73
Identity = 156/469 (33.26%), Postives = 250/469 (53.30%), Query Frame = 0

Query: 58  LPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYGKVFKSHIFGSPTIVSTDAEVSK 117
           LP G  GWPF+GETI ++    +     FM +    YGK+++S++FG PTIVS DA +++
Sbjct: 39  LPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLNR 98

Query: 118 FILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRIHGLVGAFFKSPHLKAQITKDME 177
           FILQ++ + F  SYP+S+  ++GK S+L++ G +H+ +  +   F     L+  + KD+E
Sbjct: 99  FILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDVE 158

Query: 178 SYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRRAYGFPSEHTRKQALPIVAGKL- 237
            +    + +W +N+    QDE K    +      +        +E  +K+ +  + G + 
Sbjct: 159 RHTLFVLDSWQQNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGVVS 218

Query: 238 -------------------VVKVKKIIQERKSSGISPVPKDVMEVLLKDESE-------- 297
                              ++K  +   E +   I    ++  EV  +DE+E        
Sbjct: 219 APLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDHVR 278

Query: 298 -QLTD-----------NLIAENMIDMMI----PGEDSVPILMTLAVNYLSDCPAALQHLT 357
            Q TD           NL  E ++D+++     G ++  + + LA+ +L  CP A++ L 
Sbjct: 279 KQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEELR 338

Query: 358 EENLRMKRLKEQLRES-LHWSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIKGYL 417
           EE+L + R K++L ES L+W DY  + FTQ VI ETLR+GNV+  + RKA+KD++ KGY 
Sbjct: 339 EEHLEIARAKKELGESELNWDDYKKMDFTQCVINETLRLGNVVRFLHRKALKDVRYKGYD 398

Query: 418 IPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSC------------NFTPFGGG 469
           IP GW VL    +VHLD++ YD P  FNPWRWQ ++  +             N+ PFGGG
Sbjct: 399 IPSGWKVLPVISAVHLDNSRYDQPNLFNPWRWQQQNNGASSSGSGSFSTWGNNYMPFGGG 458

BLAST of Sgr025287 vs. TAIR 10
Match: AT5G38970.1 (brassinosteroid-6-oxidase 1 )

HSP 1 Score: 264.2 bits (674), Expect = 2.9e-70
Identity = 156/453 (34.44%), Postives = 246/453 (54.30%), Query Frame = 0

Query: 40  IIVYRNCFRLLRSNFC----SQLPQGTLGWPFIGETIEFISCAYSDRPETFMDKRRRLYG 99
           +I+   C  LLR N      + LP GT+GWP  GET EF+      +   FM  +R  YG
Sbjct: 12  LIIVSLCSALLRWNQMRYTKNGLPPGTMGWPIFGETTEFLK-----QGPNFMRNQRLRYG 71

Query: 100 KVFKSHIFGSPTIVSTDAEVSKFILQSDAKAFVPSYPKSLTELMGKSSILLINGSLHKRI 159
             FKSH+ G PT++S D+EV+++IL++++K  VP YP+S+ +++G  ++  ++GS H+ +
Sbjct: 72  SFFKSHLLGCPTLISMDSEVNRYILKNESKGLVPGYPQSMLDILGTCNMAAVHGSSHRLM 131

Query: 160 HGLVGAFFKSPHLKAQITKDMESYVKLSMANWTENNPIYLQDETKNDGVSQATVSRIHRR 219
            G + +   S  ++  I   ++ +++  +  W E   I +QD+TK+     A +S + + 
Sbjct: 132 RGSLLSLISSTMMRDHILPKVDHFMRSYLDQWNELEVIDIQDKTKH----MAFLSSLTQI 191

Query: 220 AYGFPS---EHTRKQALPIVAGKLVVKV---------------------KKIIQERKSSG 279
           A        E  +     +V G L V +                     ++++QER+ SG
Sbjct: 192 AGNLRKPFVEEFKTAFFKLVVGTLSVPIDLPGTNYRCGIQARNNIDRLLRELMQERRDSG 251

Query: 280 ISPVPKDVMEVLLKDESEQ--LTDNLIAENMIDMMIPGEDSVPILMTLAVNYLSDCPAAL 339
                 D++  L+K E  +  LTD  I + ++ ++  G ++V     +A+ YL D P AL
Sbjct: 252 --ETFTDMLGYLMKKEGNRYPLTDEEIRDQVVTILYSGYETVSTTSMMALKYLHDHPKAL 311

Query: 340 QHLTEENLRMKRLKEQLRESLHWSDYLSLPFTQSVITETLRMGNVITGVMRKAMKDMKIK 399
           Q L  E+L  +  K Q  E L   D  S+ FT++VI ET R+  ++ GV+RK  +D++I 
Sbjct: 312 QELRAEHLAFRERKRQ-DEPLGLEDVKSMKFTRAVIYETSRLATIVNGVLRKTTRDLEIN 371

Query: 400 GYLIPKGWSVLTYFRSVHLDDNHYDWPYQFNPWRWQDRDLSSCNFT-PFGGGQRLCPGLD 459
           GYLIPKGW +  Y R ++ D N Y+ P  FNPWRW  + L S N    FGGG RLCPG +
Sbjct: 372 GYLIPKGWRIYVYTREINYDANLYEDPLIFNPWRWMKKSLESQNSCFVFGGGTRLCPGKE 431

Query: 460 LARLEASIFLHHFVTEFRW-MVEEDRIINFPTV 461
           L  +E S FLH+FVT +RW  +  D ++ FP V
Sbjct: 432 LGIVEISSFLHYFVTRYRWEEIGGDELMVFPRV 452

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038898401.12.6e-28675.53LOW QUALITY PROTEIN: cytochrome P450 90D2-like [Benincasa hispida][more]
KAE8646515.13.2e-28474.65hypothetical protein Csa_016429 [Cucumis sativus][more]
XP_011659645.14.1e-28474.47LOW QUALITY PROTEIN: 3-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 [Cucumis... [more]
KAA0059438.12.7e-28374.47cytochrome p450 [Cucumis melo var. makuwa][more]
ADN34104.11.3e-28274.75cytochrome p450 [Cucumis melo subsp. melo][more]
Match NameE-valueIdentityDescription
Q94IA62.6e-14860.273-epi-6-deoxocathasterone 23-monooxygenase CYP90D1 OS=Arabidopsis thaliana OX=37... [more]
Q94IW55.3e-14155.38Cytochrome P450 90D2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90D2 PE=1 SV... [more]
Q9M0667.2e-12250.343-epi-6-deoxocathasterone 23-monooxygenase CYP90C1 OS=Arabidopsis thaliana OX=37... [more]
Q425691.5e-8740.32Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1[more]
Q5CCK11.7e-7537.00Cytochrome P450 90A4 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90A4 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A5A7UW931.3e-28374.47Cytochrome p450 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold242G0016... [more]
E5GCA56.4e-28374.75Cytochrome p450 OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1[more]
A0A0A0K7W88.8e-21681.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G451390 PE=3 SV=1[more]
A0A1S3CGU62.7e-21281.413-epi-6-deoxocathasterone 23-monooxygenase OS=Cucumis melo OX=3656 GN=LOC1035007... [more]
A0A7N2LKP81.6e-18871.58Uncharacterized protein OS=Quercus lobata OX=97700 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G13730.11.9e-14960.27cytochrome P450, family 90, subfamily D, polypeptide 1 [more]
AT4G36380.15.1e-12350.34Cytochrome P450 superfamily protein [more]
AT5G05690.11.1e-8840.32Cytochrome P450 superfamily protein [more]
AT3G50660.14.8e-7333.26Cytochrome P450 superfamily protein [more]
AT5G38970.12.9e-7034.44brassinosteroid-6-oxidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 309..329
NoneNo IPR availablePANTHERPTHR24286:SF303-EPI-6-DEOXOCATHASTERONE 23-MONOOXYGENASE CYP90D1coord: 35..468
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 35..468
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 91..110
score: 25.43
coord: 342..360
score: 23.95
coord: 414..424
score: 51.88
coord: 382..406
score: 25.92
coord: 424..447
score: 32.04
IPR001128Cytochrome P450PRINTSPR00385P450coord: 424..435
score: 42.08
coord: 415..424
score: 54.49
coord: 343..354
score: 37.32
coord: 164..177
score: 13.27
IPR001128Cytochrome P450PFAMPF00067p450coord: 59..184
e-value: 1.1E-5
score: 24.4
coord: 240..453
e-value: 1.2E-42
score: 146.2
IPR004895Prenylated rab acceptor PRA1PFAMPF03208PRA1coord: 547..687
e-value: 3.8E-46
score: 156.3
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 52..470
e-value: 1.1E-82
score: 280.2
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 57..468
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 417..426

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025287.1Sgr025287.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016132 brassinosteroid biosynthetic process
biological_process GO:0010268 brassinosteroid homeostasis
biological_process GO:0016125 sterol metabolic process
biological_process GO:0016192 vesicle-mediated transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen