Sgr025268 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr025268
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionLRR receptor-like serine/threonine-protein kinase IOS1
Locationtig00004836: 1025305 .. 1029753 (-)
RNA-Seq ExpressionSgr025268
SyntenySgr025268
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATGATAATGGGAACATCAGGGCATACCCTCTTTGGTTTCCTGCTAACTGGTCTGCCACTGGCTCTTCTACTTGTTGTTCAAGCTCAGGATCAGTCAGGTAACAAAGTTATCTCCATTGTTGATTAATCTATCCAACTTCTTCCAATCATCTATATATAGAGACTTGAAGCTTAAGTTGTCTGTTATTTACTTCTCAGGCTTCATTAGTTTAGATTGTGGATCACCGGAGGGTACGAGTTACCCCGAAACGACAACCAATATTAGCTATGTTTCAGATGCATCCTACATAAACAGTGGTGTAAGTGAAAACGTAACTTCGACATATAGAGATTCCCATCAAAGGCAAATGCTGAAGCTTAGAAGCTTTCCTCAAGGAATCAGAAACTGTTACAATGTAAGCGTTGTAAAGGGTACCAAATACTTGATCCGAGCGAGTTTCTTGTACGGGAATTATGATGGCCTGAATTCGCTACCAAAATTTGATCTATATGTTGGGAATAGCTTGTGGGAGACAGTAAACATCACTGACGAAGGCATAGATGTTTTCAAAGATCTCATACATATTACCTCCTCAAATGAAGTTCAAATTTGTCTGATCAATACAGGGAATGGAGTGCCGTTTATTTCAGCATTAGAATTTAGACCTTTACTCAACATCACTTATCAAACAGTGTCGGGATCCTTATCACTTCATGCTCGACTGGACATTGGTTCAACTGATTATCGAAATTTCAGGTATTTAAGCTCTGAGTTTACAAACAAATTTACTGCGAATAAAAAGAGGATATGCTTTAACAAGTGAGATGCTAACATAATATTTTGTTTATGTGCCTACTCTCTCTTAAGATTCCCTTTTGATGTTTATGATCGCATCTGGTCAGCATATAACTTCAAGGAGTGGACACAAGTAAGCACCAAGCATGCTGTAGATGATAGTGGGCCCAACAATCACCGACTGCCGTCCATTGTCATGAAAACTGCTTCCGCTCCAACAAATGCAAGCAACCCGGTAGAATTTTGGTGGGATACATTAGATTCATCCCAATATTATGTATTTATGCATTTTGCTGAAGTTGTAAAGCTTCAACCCAACCAGAGTAGAGGGTTCAATATCACTCACAACGGGGATCTTTTTTATGGACCTCTAGTTCCAAATTATTTGTCCACGTTGTCTGTTTTCAGTGAAAATCCATTGGATGCAGCAAATAGGCATTTATTTTCTTTCATTCCAATCAAGAACGCAACACTTCCTCCTATCATCAATGCTCTCGAGATATATACAGTAAAAAACATATCAGAGTTTGAAGCAGACCAAGGAGATGGTATCCTTCTTTAGATGCACTTTGTGAGTTACTTTATTGGAAGTTAATGAATATTAATTTGATTTCTTCTTTGAATAATTAGTTGATGCAATTACCAAGATCAAGTCAACTTATGGAGTAAAGAGGGATTGGCAAGGAGATCCATGTGTGCCAATGGGATATCCCTGGAGTGGATTAATTTGTAGCAATGAAACTGCTCCAAGAATTATATCCTTGTAAGTGTTAGCCTTCTTCAAGCTTAAAAGTTAAAATTGTCTTCGTATTCGAAGGATGGATCTCATGAATTCATGAAAAAAATGTGAATTTTGTGAACAGGAACCTATCTGCAAGTGGACTGATAGGTGAAATATCTTCTGACATATCAAATCTAACAATGTTGCAAACTTTGTAAGTGTCTCTCTATTAGGAATCTTAGTAGGGAAATATTAGGGATATTATTACGATATTAGGTCGGCATAATTGTCATTAGATAAAGTGTTCGTTAGAAAAGTGGTTATACATACAATAGGGCAGAGGGATTTGAGGTATGCCGAATTTGGAGAGCAGTTTTTGGGAGAGTTAGTGTTTGGATATTGTTGATTTCCTTTCTTATTTCAATACTATTTTCTATCCATTTCTATTGCAATTTTTGGGTCCTATCATATCCGTAGATGAGTTTATGCTAACTTAATTTCCTGTTCTCTATCAGGGACTTGTCCTACAATGAGTTGACCGGAGAGCTGCCGGAGTTCTTAGTAAATTTTCCAAATTTAAGAAACCTGTAAGTTTCTTAGTATTAATTTCCTAAACAAAAAAGTTTCTTAGTTTTAATCCTGACATCCAAAACATTAAATTCCAGGTGAAATTTGAAGGTTATCTATCTCTTACTGTGTTTGCAGTAATTTAAGCAGAAACAAGTTCACAGGCTCAGTTCCTAAGGCTCTCTTGCAAAGGGCTGAAGTTGGATCACTTACATTAAGGTTAGCTATCAAAATGAAGTTCATTTCCCCTCACTTTATTCGAAGTTATCATTTTCTTTGTTTCCTTTACTTCCATGTTGTTGCCTTCTAATTTTTTTGTTTTCATATTAAATGACTAACAGAAAAGACAAATGAAAATTCATTTGCTTAAACCTGACATCGTCTTCTGCTTGTGAAAGTGTGGGTGAAAATCCAGATCTTTGCACGTCACTTAAATGCTATAACAAGAGGAAGAAGTACTTGGCACTTATAATTTTTTCCAGTGTAATGGCTGCGCTCCTACCTATCCTAATGGTTACTCTTGTTATCTACAAAAGGAGAAAACAAAGAGGCAAGTTTTTCATTTCTTCTGCTTCTGTTTCTATAATTTATATCTGTTTATAACACATATCTTTAGATTACTATTTCCAGCATATTTCAAGTAAATGCAATGTAACTGAGGTAAACAGGGAAAAGAGTTCCTAAGTGATGATTGACTTCAAAGGTGTAGAATGTACCAAAAATAAAATGTGTTTATTTTTCTTGGTGCAGAGAATCTCAGAAGATCAATGGAAGAAAGGTTACTTAAATTGAAGAACCGACAAGTTAGGTACTCTGAGATACTGGTTATCACAGATAACTTGAAGACAACTATTGGGGAAGGAGGATTTGGAAAAGTATACTTGGGTGTACTGAGTGATAAAACCCAAGTTGCTATCAAGTTGCTGTCTGCATCGTCACGGCAAGGCTCCAATGAATTCATAGCAGAGGTTCAGTTTTTCTATTGTTCAAATTTGCTTGTACTAAGTTAACTAGTATATGATATATCGTGTTTGCAAATGCAGGCTCAAATATTAACAATTGTTCATCATAGAAATCTGGTTTCTCTCATTGGTTACTGTGATGAGGTTGAGAATAAGGCGCTCATTTACGAATTCATGGCTAATGGAAATCTACGCAAGCATCTATCTGGTAATTATTATAGTGTCTTACAAAAGAAGATTTCTCTCAAATGTATGTATCCTGATGATTGGTTGATTATATAAGATGATTTTGTTTCCCTCTTTTGCTGATATAGATTCAAGCACAAAGGCCTTGAGTTGGATGGAAAGACTTCAAATTGCAGTTGATGCAGCACAAGGTGTACATAAATAACACAACGCCGCAATAGTTCATACTTTTATTTACATACTCTTCTGGTTTGTGACATCTAGTAAACAAACTGCAGGGCTGGAATATTTGCACAGTAATTGTAAGCCCCCTATAATTCACAGAGATATGAAGACTTCAAATATCTTACTGAATGAAAGAATGCAAGCTAAAATATCAGATTTTGGACTGTCTAGGGTATTTGCAAATGAAAGTGATACCCATTTGTCCACATCTCCTGCTGGCACCTTCGGATATGTCGACCCTACGTAAGTATCAATGACCAATGCATTGCTCATGTGCCCGACTAAATGAGTTATCTGATGCTGCAACGTAATGAAATAAGTATATGATATCATTATTTGGTTAATGATGTCAATGGCAAGGACTACATAAAATTACAATTCAAAATAATCAAAATCTCTTAAAGTTTAGGGATCAAATAAATACCACCAACAAGGCTTAATGACTAAATTGTAATGTAACCTACGTTTTGCCTTCTATTCAAGCCAAATTCAATGTTTGGCTTGTCAGTCTCTTTATTTTGAAGTGTCCCAATATGACAAAAGAAGCATCTTGTGTACACAAACCAAAGAATTACACAAAAAATTAAGACATAAATTTGTTGCAGGATTCATTTATCTGGAAACTTCACCAAGAAAAGTGATGTTTACAGCTTTGGAATTGTGTTGTTTGAGCTGGTGAGTGGCCAGCCTGCCATAATCAAGGGATCTGAATACAACATCCACATAGTGGACTGGGCTAAACCTCTTATTGCAGAAGGCAACATCCAAAACATGGTTGATCCAAGGTTGGAAGGCTCTATAGAAACCTGTTCCGCTGGAAAATTTGTGGAGCTGGCTTTGTCGTGTACATTGTCAACTTCAGCAGAAAGGCCCGACATGAGTGATGTGGTGTCACAACTCATTGAATGTCTGAAGATGGCTCGGGACAGAACTCCACAGATGCCACCTAACAACACTGAGAACTTCTCATACAATTCAATTGGCTCTGAATCAATTTTGAGTCCTAGATAG

mRNA sequence

ATGATGATAATGGGAACATCAGGGCATACCCTCTTTGGTTTCCTGCTAACTGGTCTGCCACTGGCTCTTCTACTTGTTGTTCAAGCTCAGGATCAGTCAGGCTTCATTAGTTTAGATTGTGGATCACCGGAGGGTACGAGTTACCCCGAAACGACAACCAATATTAGCTATGTTTCAGATGCATCCTACATAAACAGTGGTGTAAGTGAAAACGTAACTTCGACATATAGAGATTCCCATCAAAGGCAAATGCTGAAGCTTAGAAGCTTTCCTCAAGGAATCAGAAACTGTTACAATGTAAGCGTTGTAAAGGGTACCAAATACTTGATCCGAGCGAGTTTCTTGTACGGGAATTATGATGGCCTGAATTCGCTACCAAAATTTGATCTATATGTTGGGAATAGCTTGTGGGAGACAGTAAACATCACTGACGAAGGCATAGATGTTTTCAAAGATCTCATACATATTACCTCCTCAAATGAAGTTCAAATTTGTCTGATCAATACAGGGAATGGAGTGCCGTTTATTTCAGCATTAGAATTTAGACCTTTACTCAACATCACTTATCAAACAGTGTCGGGATCCTTATCACTTCATGCTCGACTGGACATTGGTTCAACTGATTATCGAAATTTCAGATTCCCTTTTGATGTTTATGATCGCATCTGGTCAGCATATAACTTCAAGGAGTGGACACAAGTAAGCACCAAGCATGCTGTAGATGATAGTGGGCCCAACAATCACCGACTGCCGTCCATTGTCATGAAAACTGCTTCCGCTCCAACAAATGCAAGCAACCCGGTAGAATTTTGGTGGGATACATTAGATTCATCCCAATATTATGTATTTATGCATTTTGCTGAAGTTGTAAAGCTTCAACCCAACCAGAGTAGAGGGTTCAATATCACTCACAACGGGGATCTTTTTTATGGACCTCTAGTTCCAAATTATTTGTCCACGTTGTCTGTTTTCAGTGAAAATCCATTGGATGCAGCAAATAGGCATTTATTTTCTTTCATTCCAATCAAGAACGCAACACTTCCTCCTATCATCAATGCTCTCGAGATATATACAGTAAAAAACATATCAGAGTTTGAAGCAGACCAAGGAGATGTTGATGCAATTACCAAGATCAAGTCAACTTATGGAGTAAAGAGGGATTGGCAAGGAGATCCATGTGTGCCAATGGGATATCCCTGGAGTGGATTAATTTGTAGCAATGAAACTGCTCCAAGAATTATATCCTTGAACCTATCTGCAAGTGGACTGATAGGTGAAATATCTTCTGACATATCAAATCTAACAATGTTGCAAACTTTGGACTTGTCCTACAATGAGTTGACCGGAGAGCTGCCGGAGTTCTTAGTAAATTTTCCAAATTTAAGAAACCTTAATTTAAGCAGAAACAAGTTCACAGGCTCAGTTCCTAAGGCTCTCTTGCAAAGGGCTGAAGTTGGATCACTTACATTAAGGATTCATTTATCTGGAAACTTCACCAAGAAAAGTGATGTTTACAGCTTTGGAATTGTGTTGTTTGAGCTGGTGAGTGGCCAGCCTGCCATAATCAAGGGATCTGAATACAACATCCACATAGTGGACTGGGCTAAACCTCTTATTGCAGAAGGCAACATCCAAAACATGGTTGATCCAAGGTTGGAAGGCTCTATAGAAACCTGTTCCGCTGGAAAATTTGTGGAGCTGGCTTTGTCGTGTACATTGTCAACTTCAGCAGAAAGGCCCGACATGAGTGATGTGGTGTCACAACTCATTGAATGTCTGAAGATGGCTCGGGACAGAACTCCACAGATGCCACCTAACAACACTGAGAACTTCTCATACAATTCAATTGGCTCTGAATCAATTTTGAGTCCTAGATAG

Coding sequence (CDS)

ATGATGATAATGGGAACATCAGGGCATACCCTCTTTGGTTTCCTGCTAACTGGTCTGCCACTGGCTCTTCTACTTGTTGTTCAAGCTCAGGATCAGTCAGGCTTCATTAGTTTAGATTGTGGATCACCGGAGGGTACGAGTTACCCCGAAACGACAACCAATATTAGCTATGTTTCAGATGCATCCTACATAAACAGTGGTGTAAGTGAAAACGTAACTTCGACATATAGAGATTCCCATCAAAGGCAAATGCTGAAGCTTAGAAGCTTTCCTCAAGGAATCAGAAACTGTTACAATGTAAGCGTTGTAAAGGGTACCAAATACTTGATCCGAGCGAGTTTCTTGTACGGGAATTATGATGGCCTGAATTCGCTACCAAAATTTGATCTATATGTTGGGAATAGCTTGTGGGAGACAGTAAACATCACTGACGAAGGCATAGATGTTTTCAAAGATCTCATACATATTACCTCCTCAAATGAAGTTCAAATTTGTCTGATCAATACAGGGAATGGAGTGCCGTTTATTTCAGCATTAGAATTTAGACCTTTACTCAACATCACTTATCAAACAGTGTCGGGATCCTTATCACTTCATGCTCGACTGGACATTGGTTCAACTGATTATCGAAATTTCAGATTCCCTTTTGATGTTTATGATCGCATCTGGTCAGCATATAACTTCAAGGAGTGGACACAAGTAAGCACCAAGCATGCTGTAGATGATAGTGGGCCCAACAATCACCGACTGCCGTCCATTGTCATGAAAACTGCTTCCGCTCCAACAAATGCAAGCAACCCGGTAGAATTTTGGTGGGATACATTAGATTCATCCCAATATTATGTATTTATGCATTTTGCTGAAGTTGTAAAGCTTCAACCCAACCAGAGTAGAGGGTTCAATATCACTCACAACGGGGATCTTTTTTATGGACCTCTAGTTCCAAATTATTTGTCCACGTTGTCTGTTTTCAGTGAAAATCCATTGGATGCAGCAAATAGGCATTTATTTTCTTTCATTCCAATCAAGAACGCAACACTTCCTCCTATCATCAATGCTCTCGAGATATATACAGTAAAAAACATATCAGAGTTTGAAGCAGACCAAGGAGATGTTGATGCAATTACCAAGATCAAGTCAACTTATGGAGTAAAGAGGGATTGGCAAGGAGATCCATGTGTGCCAATGGGATATCCCTGGAGTGGATTAATTTGTAGCAATGAAACTGCTCCAAGAATTATATCCTTGAACCTATCTGCAAGTGGACTGATAGGTGAAATATCTTCTGACATATCAAATCTAACAATGTTGCAAACTTTGGACTTGTCCTACAATGAGTTGACCGGAGAGCTGCCGGAGTTCTTAGTAAATTTTCCAAATTTAAGAAACCTTAATTTAAGCAGAAACAAGTTCACAGGCTCAGTTCCTAAGGCTCTCTTGCAAAGGGCTGAAGTTGGATCACTTACATTAAGGATTCATTTATCTGGAAACTTCACCAAGAAAAGTGATGTTTACAGCTTTGGAATTGTGTTGTTTGAGCTGGTGAGTGGCCAGCCTGCCATAATCAAGGGATCTGAATACAACATCCACATAGTGGACTGGGCTAAACCTCTTATTGCAGAAGGCAACATCCAAAACATGGTTGATCCAAGGTTGGAAGGCTCTATAGAAACCTGTTCCGCTGGAAAATTTGTGGAGCTGGCTTTGTCGTGTACATTGTCAACTTCAGCAGAAAGGCCCGACATGAGTGATGTGGTGTCACAACTCATTGAATGTCTGAAGATGGCTCGGGACAGAACTCCACAGATGCCACCTAACAACACTGAGAACTTCTCATACAATTCAATTGGCTCTGAATCAATTTTGAGTCCTAGATAG

Protein sequence

MMIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRGFNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLSASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEVGSLTLRIHLSGNFTKKSDVYSFGIVLFELVSGQPAIIKGSEYNIHIVDWAKPLIAEGNIQNMVDPRLEGSIETCSAGKFVELALSCTLSTSAERPDMSDVVSQLIECLKMARDRTPQMPPNNTENFSYNSIGSESILSPR
Homology
BLAST of Sgr025268 vs. NCBI nr
Match: XP_022152419.1 (LRR receptor-like serine/threonine-protein kinase IOS1 [Momordica charantia])

HSP 1 Score: 986.9 bits (2550), Expect = 8.1e-284
Identity = 538/879 (61.21%), Postives = 571/879 (64.96%), Query Frame = 0

Query: 2   MIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDA 61
           MIM T GH+LFGFLL      +LL+VQAQDQS FISLDCG PEGTSY ET+TNISY+SDA
Sbjct: 1   MIMETPGHSLFGFLL------ILLLVQAQDQSDFISLDCGLPEGTSYTETSTNISYISDA 60

Query: 62  SYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDG 121
           SYINSGVSENVT TYRDSHQRQMLKLRSFPQGIRNCYNVS+VKGTKYLIRASFLYGNYDG
Sbjct: 61  SYINSGVSENVTWTYRDSHQRQMLKLRSFPQGIRNCYNVSIVKGTKYLIRASFLYGNYDG 120

Query: 122 LNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEF 181
           LN LP FDLYVGNSLWETVNITD+GID+ KDLIHITS NEV ICLI+TGNGVPFISALEF
Sbjct: 121 LNLLPTFDLYVGNSLWETVNITDKGIDIVKDLIHITSLNEVHICLIDTGNGVPFISALEF 180

Query: 182 RPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVD 241
           RPLLN+TYQTVSGSLSL ARLD+GSTDY+NFRFPFDVYDRIW AYNF+ WTQVSTKH VD
Sbjct: 181 RPLLNMTYQTVSGSLSLAARLDVGSTDYQNFRFPFDVYDRIWPAYNFEGWTQVSTKHTVD 240

Query: 242 DSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRGFN 301
            SG NNH+LPSIVMKTAS P N S P EFWWDT+DSSQYYVFMHFAEVV+LQPNQ R FN
Sbjct: 241 PSG-NNHQLPSIVMKTASTPINGSKPAEFWWDTIDSSQYYVFMHFAEVVELQPNQIRAFN 300

Query: 302 ITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKN 361
           ITHNGDL++GP+VPNYLSTLSVFSE PL+AANRHLFSFI IKN TLPPI+NALEIYT KN
Sbjct: 301 ITHNGDLYFGPIVPNYLSTLSVFSEKPLEAANRHLFSFIAIKNTTLPPIVNALEIYTAKN 360

Query: 362 ISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLSAS 421
           ISEFEADQGDVDAIT IKSTYGVKRDWQGDPCVP+ YPWSGL CSNET PRIISLNLSAS
Sbjct: 361 ISEFEADQGDVDAITNIKSTYGVKRDWQGDPCVPIRYPWSGLTCSNETTPRIISLNLSAS 420

Query: 422 GLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQ 481
            LIGEISS ISNLTMLQTLDLSYNELTGELPEFLVNFPNLRN+NL+RNKFTGSVPKALLQ
Sbjct: 421 ELIGEISSYISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNINLTRNKFTGSVPKALLQ 480

Query: 482 RAEVGSLTL--------------------------------------------------- 541
           RAE GSLTL                                                   
Sbjct: 481 RAEAGSLTLSVGENPDLCTSLKCDNKRKNNKKKKHLLLIILSTIISVILPILMGILVIYK 540

Query: 542 ------------------------------------------------------------ 601
                                                                       
Sbjct: 541 RRKQRENLRRSIEERLLKSKNRQVRYSEILFISDNLKTVIGEGGFGKVYLGAVSDRTQVA 600

Query: 602 ------------------------------------------------------------ 626
                                                                       
Sbjct: 601 IKLLSKASRQGYNEFRAEAQILTVVHHKNLVALIGYCDEAENKALIYELMANGNLRKHLS 660

BLAST of Sgr025268 vs. NCBI nr
Match: XP_022155109.1 (probable LRR receptor-like serine/threonine-protein kinase At1g05700 [Momordica charantia])

HSP 1 Score: 878.2 bits (2268), Expect = 4.1e-251
Identity = 481/881 (54.60%), Postives = 547/881 (62.09%), Query Frame = 0

Query: 2   MIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDA 61
           MI+GTSGH+LFGFLLTGL L LLL+VQAQDQSGFISLDCG PEGT Y ETTT I+YVSDA
Sbjct: 1   MIVGTSGHSLFGFLLTGLSLPLLLLVQAQDQSGFISLDCGLPEGTRYNETTTGINYVSDA 60

Query: 62  SYINSGVSENVTSTY--RDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNY 121
           S+INSGVS++V S Y   D++ RQ+  LRSFPQGIRNCYNVS+VKGT+YLIRASFLYGNY
Sbjct: 61  SFINSGVSKSVASAYGEGDTYPRQLRMLRSFPQGIRNCYNVSIVKGTEYLIRASFLYGNY 120

Query: 122 DGLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISAL 181
           DGL+SLP FDLY+GNSLW+T+  T   +D F DLIH+TSSNEV ICLINTGNGVPFISAL
Sbjct: 121 DGLDSLPMFDLYIGNSLWQTLKFTSNAMDAFIDLIHVTSSNEVHICLINTGNGVPFISAL 180

Query: 182 EFRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHA 241
           EFR  LNIT+QTV  SLSL+ R+DIGS + RN+RFP DVYDRIWS+YN  EWTQVST   
Sbjct: 181 EFRASLNITFQTVVTSLSLYTRMDIGSIEDRNYRFPLDVYDRIWSSYNSNEWTQVSTNLT 240

Query: 242 VDDSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRG 301
           VD  G N  RLPSIVM+TAS P NAS P+E WWD++DSSQYYVFMHFAE VK + NQ+R 
Sbjct: 241 VDALGGNRLRLPSIVMETASTPKNASKPLEIWWDSVDSSQYYVFMHFAEFVKPRANQTRE 300

Query: 302 FNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTV 361
           FNIT+NGD FYGP++PNYLS++++ +++PL+AANR+LFSF P +N+TLPPIINA EIYTV
Sbjct: 301 FNITYNGDFFYGPVIPNYLSSMTISTQDPLEAANRYLFSFTPTENSTLPPIINAFEIYTV 360

Query: 362 KNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLS 421
           K++SE +A+QGDVDAIT IKS+YG+KRDWQGDPCVPM YPWSGL CSN+TAPRIISLNLS
Sbjct: 361 KHVSELKANQGDVDAITNIKSSYGIKRDWQGDPCVPMRYPWSGLNCSNQTAPRIISLNLS 420

Query: 422 ASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKAL 481
           +SGL GEISS ISNLTMLQTLDLS NELTGELPEFLVN PNLR +NL+RNKFTGS+PKAL
Sbjct: 421 SSGLTGEISSYISNLTMLQTLDLSNNELTGELPEFLVNLPNLRIINLTRNKFTGSIPKAL 480

Query: 482 LQRAEVGSLTL------------------------------------------------- 541
           LQRAE GSLTL                                                 
Sbjct: 481 LQRAEAGSLTLSVGENPDLCTALKCDNKRNNNKKKKHLVLIILSCIIAVILPILMVSLVI 540

Query: 542 ------------------------------------------------------------ 601
                                                                       
Sbjct: 541 YKRRKQRENLKISVQERLLKSKNQQVHYSEILIITENLKTTIGEGGFGKVYLGVLSDKTQ 600

Query: 602 ------------------------------------------------------------ 626
                                                                       
Sbjct: 601 VAVKLLSAMSQQGYNEFRAEAQILTVVHHRNLVSLIGYCDEAENKALIYEFMGNGNLRDH 660

BLAST of Sgr025268 vs. NCBI nr
Match: XP_022144106.1 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X2 [Momordica charantia])

HSP 1 Score: 860.9 bits (2223), Expect = 6.7e-246
Identity = 474/835 (56.77%), Postives = 532/835 (63.71%), Query Frame = 0

Query: 1   MMIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSD 60
           MMIMG+SGH+LF F      L+L+L+VQAQDQSGFISLDCGSPEGTSY E TTNI+Y+SD
Sbjct: 1   MMIMGSSGHSLFAF------LSLILLVQAQDQSGFISLDCGSPEGTSYTEMTTNINYISD 60

Query: 61  ASYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYD 120
           A YINSGVSE+V   Y D++ RQ+  LRSFPQG+RNCYNVS+VKGTKYLIRASFLYGNYD
Sbjct: 61  AFYINSGVSESVALAYGDTNPRQLRNLRSFPQGVRNCYNVSIVKGTKYLIRASFLYGNYD 120

Query: 121 GLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALE 180
           GL+ LPKFDLYVG+SLWETVNITD+G+DVFKDLIH+TS NEV +CLINTGNGVPFISALE
Sbjct: 121 GLDLLPKFDLYVGSSLWETVNITDKGMDVFKDLIHMTSLNEVHMCLINTGNGVPFISALE 180

Query: 181 FRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAV 240
           FRPLLNITYQ  SGSLSL +R+DIGSTD R +RF FDVYDRIWS++     T+ ST   V
Sbjct: 181 FRPLLNITYQRKSGSLSLFSRMDIGSTDNRAYRFSFDVYDRIWSSFEDNVLTRESTNLTV 240

Query: 241 DDSGPNNHRLPSIVMKTASAPTNAS-NPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRG 300
           D    + +++PS+VMKTA  P       +EF W+T+DSSQYYVF+HFAE+VKLQPNQSR 
Sbjct: 241 DAIRRDGYQMPSVVMKTAYTPMGIGWEAMEFGWETVDSSQYYVFLHFAEIVKLQPNQSRV 300

Query: 301 FNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTV 360
           FNITHNG+ FYGPL+P+YLST +V SENPLDAA+ H+FSFIPI+NATLPPIINALEIY+V
Sbjct: 301 FNITHNGNHFYGPLIPSYLSTQTVSSENPLDAADSHIFSFIPIENATLPPIINALEIYSV 360

Query: 361 KNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLS 420
           K+ISE EA+QGDVDAIT IKSTY + RDWQGDPCVPMGYPWSGL CSNETAPRIISLNLS
Sbjct: 361 KDISELEANQGDVDAITNIKSTYAINRDWQGDPCVPMGYPWSGLHCSNETAPRIISLNLS 420

Query: 421 ASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKAL 480
           AS L GEISS ISNL MLQTLDLS NELTGELPEFLVN PNLR LNL++NKFTGSVPKAL
Sbjct: 421 ASELTGEISSYISNLAMLQTLDLSDNELTGELPEFLVNLPNLRTLNLTKNKFTGSVPKAL 480

Query: 481 LQRAEVGSLTL------------------------------------------------- 540
           LQRAE GSLTL                                                 
Sbjct: 481 LQRAEAGSLTLSADENPDLCTSLKCHNKWKKYLPLMILSTIIALLLPILMVTLIFYKKRK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 QRENLKRSVEERFLKSKNRQIQYSEILAITDNLKTTIGEGGFGKAQILTIAHHKNLVSLI 600

Query: 601 ------------------------------------------------------------ 616
                                                                       
Sbjct: 601 GYCDEAENKALVYEFMGNGNLRNHLLDSSTKVLSWMERLQIAVDAAQGLEYLHNGCKPPI 660

BLAST of Sgr025268 vs. NCBI nr
Match: XP_022144097.1 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X1 [Momordica charantia])

HSP 1 Score: 849.0 bits (2192), Expect = 2.7e-242
Identity = 474/866 (54.73%), Postives = 532/866 (61.43%), Query Frame = 0

Query: 1   MMIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSD 60
           MMIMG+SGH+LF F      L+L+L+VQAQDQSGFISLDCGSPEGTSY E TTNI+Y+SD
Sbjct: 1   MMIMGSSGHSLFAF------LSLILLVQAQDQSGFISLDCGSPEGTSYTEMTTNINYISD 60

Query: 61  ASYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYD 120
           A YINSGVSE+V   Y D++ RQ+  LRSFPQG+RNCYNVS+VKGTKYLIRASFLYGNYD
Sbjct: 61  AFYINSGVSESVALAYGDTNPRQLRNLRSFPQGVRNCYNVSIVKGTKYLIRASFLYGNYD 120

Query: 121 GLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALE 180
           GL+ LPKFDLYVG+SLWETVNITD+G+DVFKDLIH+TS NEV +CLINTGNGVPFISALE
Sbjct: 121 GLDLLPKFDLYVGSSLWETVNITDKGMDVFKDLIHMTSLNEVHMCLINTGNGVPFISALE 180

Query: 181 FRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAV 240
           FRPLLNITYQ  SGSLSL +R+DIGSTD R +RF FDVYDRIWS++     T+ ST   V
Sbjct: 181 FRPLLNITYQRKSGSLSLFSRMDIGSTDNRAYRFSFDVYDRIWSSFEDNVLTRESTNLTV 240

Query: 241 DDSGPNNHRLPSIVMKTASAPTNAS-NPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRG 300
           D    + +++PS+VMKTA  P       +EF W+T+DSSQYYVF+HFAE+VKLQPNQSR 
Sbjct: 241 DAIRRDGYQMPSVVMKTAYTPMGIGWEAMEFGWETVDSSQYYVFLHFAEIVKLQPNQSRV 300

Query: 301 FNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTV 360
           FNITHNG+ FYGPL+P+YLST +V SENPLDAA+ H+FSFIPI+NATLPPIINALEIY+V
Sbjct: 301 FNITHNGNHFYGPLIPSYLSTQTVSSENPLDAADSHIFSFIPIENATLPPIINALEIYSV 360

Query: 361 KNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLS 420
           K+ISE EA+QGDVDAIT IKSTY + RDWQGDPCVPMGYPWSGL CSNETAPRIISLNLS
Sbjct: 361 KDISELEANQGDVDAITNIKSTYAINRDWQGDPCVPMGYPWSGLHCSNETAPRIISLNLS 420

Query: 421 ASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKAL 480
           AS L GEISS ISNL MLQTLDLS NELTGELPEFLVN PNLR LNL++NKFTGSVPKAL
Sbjct: 421 ASELTGEISSYISNLAMLQTLDLSDNELTGELPEFLVNLPNLRTLNLTKNKFTGSVPKAL 480

Query: 481 LQRAEVGSLTL------------------------------------------------- 540
           LQRAE GSLTL                                                 
Sbjct: 481 LQRAEAGSLTLSADENPDLCTSLKCHNKWKKYLPLMILSTIIALLLPILMVTLIFYKKRK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 QRENLKRSVEERFLKSKNRQIQYSEILAITDNLKTTIGEGGFGKVYLGVLSDKTKVAVKL 600

Query: 601 ------------------------------------------------------------ 616
                                                                       
Sbjct: 601 LSGSSQQGSNEFKAEAQILTIAHHKNLVSLIGYCDEAENKALVYEFMGNGNLRNHLLDSS 660

BLAST of Sgr025268 vs. NCBI nr
Match: XP_038896176.1 (putative leucine-rich repeat receptor-like serine/threonine-protein kinase At2g19230 isoform X3 [Benincasa hispida])

HSP 1 Score: 803.5 bits (2074), Expect = 1.3e-228
Identity = 453/880 (51.48%), Postives = 520/880 (59.09%), Query Frame = 0

Query: 3   IMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDAS 62
           +MGTSGH LFG LL+GL LA LL VQAQDQSGFISLDCG PEGTSY ETTT I+YVSDAS
Sbjct: 66  LMGTSGHCLFGILLSGLALA-LLHVQAQDQSGFISLDCGLPEGTSYTETTTKINYVSDAS 125

Query: 63  YINSGVSENVTSTY--RDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYD 122
           +INSGVS++V S Y   D++ +Q+ KLRSFPQGIRNCY+VS VKGT+YLIRASFLYGNYD
Sbjct: 126 FINSGVSQDVASVYGDGDTYPKQLRKLRSFPQGIRNCYSVSTVKGTEYLIRASFLYGNYD 185

Query: 123 GLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALE 182
           GL++LP FDLY+ NSLW+T+N +D G+D + DLIH+TSSN V+ICLINTGNGVPFISALE
Sbjct: 186 GLDTLPMFDLYIENSLWQTLNFSDNGMDAYIDLIHVTSSNAVRICLINTGNGVPFISALE 245

Query: 183 FRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAV 242
           FRP LNITY T+S SLSL+ R+DIGST+ + +RFPFDVYDRIWS +N  EWTQVST  +V
Sbjct: 246 FRPSLNITYLTISSSLSLYTRMDIGSTEDQKYRFPFDVYDRIWSPFNSNEWTQVSTNLSV 305

Query: 243 DDSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRGF 302
           +  G N  +LPSIVM+TAS P N S P+E WWD +DSSQYY+ MH AEV++ + N+SR F
Sbjct: 306 EPIGNNGLQLPSIVMQTASTPKNKSKPLEIWWDAIDSSQYYILMHVAEVLRPEVNKSREF 365

Query: 303 NITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVK 362
            IT+NGD   GP++PNYLST+S+F + PL+ ANRH+ SFI  ++ATLPPIINA E+Y  K
Sbjct: 366 IITYNGDFVLGPIIPNYLSTMSIFPKEPLEGANRHVISFISTEDATLPPIINAFELYIGK 425

Query: 363 NISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLSA 422
           NIS+ EADQGDVDAIT IKSTYG+K+DWQGDPCVPMG+PWSGL CSN T PRIISLNLS 
Sbjct: 426 NISKLEADQGDVDAITNIKSTYGIKKDWQGDPCVPMGFPWSGLNCSNGTVPRIISLNLST 485

Query: 423 SGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALL 482
           SGL GEIS  ISNLTMLQ LDLS NELTGELPE LV  PNLR L L+RN+FTG +PK LL
Sbjct: 486 SGLTGEISPYISNLTMLQILDLSDNELTGELPE-LVKLPNLRILYLTRNRFTGLIPKDLL 545

Query: 483 QRAEVGSLTL-------------------------------------------------- 542
            RAE G LTL                                                  
Sbjct: 546 LRAEAGLLTLSVGENPDLCTSLECVNKRKNNKRKKYLVAIILSSIIAVLLPIFMVTLVIY 605

Query: 543 ------------------------------------------------------------ 602
                                                                       
Sbjct: 606 KRRKQRGNLKRSIQERLLKSKTHLVHFSEILVITDNLKTTIGEGGFGKVYLGVLSDKTQV 665

Query: 603 ------------------------------------------------------------ 626
                                                                       
Sbjct: 666 AVKLMSSMSQQGYNEFRAEAQILTVVHHINLVSLIGYCDEAENKALIYEFMGNGSLRDFL 725

BLAST of Sgr025268 vs. ExPASy Swiss-Prot
Match: Q9C8I6 (LRR receptor-like serine/threonine-protein kinase IOS1 OS=Arabidopsis thaliana OX=3702 GN=IOS1 PE=1 SV=1)

HSP 1 Score: 450.7 bits (1158), Expect = 2.8e-125
Identity = 241/487 (49.49%), Postives = 329/487 (67.56%), Query Frame = 0

Query: 14  FLLTGLPL-ALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENV 73
           FLL  L + + LL+  AQDQSGFISLDCGSP  TS+ E TTNI+Y+SDA++IN+GV  ++
Sbjct: 7   FLLVLLQIFSALLLCLAQDQSGFISLDCGSPRETSFREKTTNITYISDANFINTGVGGSI 66

Query: 74  TSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDGLNSLPKFDLYV 133
              YR   Q+Q   LRSFPQGIRNCY +++  G +YLIRA+FL+G YD   S  +F+LY+
Sbjct: 67  KQGYRTQFQQQTWNLRSFPQGIRNCYTLNLTIGDEYLIRANFLHGGYDDKPS-TQFELYL 126

Query: 134 GNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTV 193
           G +LW TV  T+E      ++IHI +++ +QICL+ TGN  PFISALE R L+N TY T 
Sbjct: 127 GPNLWSTVTTTNETEASIFEMIHILTTDRLQICLVKTGNATPFISALELRKLMNTTYLTR 186

Query: 194 SGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPS 253
            GSL    R D+G+T  + +R+  DV+DR+W+ YNF  W+Q+ST  +V+ +  N+++ P 
Sbjct: 187 QGSLQTFIRADVGATVNQGYRYGIDVFDRVWTPYNFGNWSQISTNQSVNIN--NDYQPPE 246

Query: 254 IVMKTASAPTNASNPVEFWWDTLDSS-QYYVFMHFAEVVKLQPNQSRGFNITHNGDLFYG 313
           I M TAS PT+    +      ++ + Q+YVFMHFAE+ +L+ N +R FNI +N    YG
Sbjct: 247 IAMVTASVPTDPDAAMNISLVGVERTVQFYVFMHFAEIQELKSNDTREFNIMYNNKHIYG 306

Query: 314 PLVPNYLSTLSVF--SENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNISEFEADQ 373
           P  P   +T SVF  +E   DA  +++FS     N+TLPP++NA+EIY+V  + + E D+
Sbjct: 307 PFRPLNFTTSSVFTPTEVVADANGQYIFSLQRTGNSTLPPLLNAMEIYSVNLLPQQETDR 366

Query: 374 GDVDAITKIKSTYGV-KRDWQGDPCVPMGYPWSGLICS---NETAPRIISLNLSASGLIG 433
            +VDA+  IKS YGV K DW+GDPCVP+ Y WSG+ C+   NET P+IISL+LS SGL G
Sbjct: 367 KEVDAMMNIKSAYGVNKIDWEGDPCVPLDYKWSGVNCTYVDNET-PKIISLDLSTSGLTG 426

Query: 434 EISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEV 493
           EI   IS+LT L+ LDLS N LTG +PEFL N   L+ +NLS N+  GS+P  LL +   
Sbjct: 427 EILEFISDLTSLEVLDLSNNSLTGSVPEFLANMETLKLINLSGNELNGSIPATLLDKERR 486


HSP 2 Score: 89.4 bits (220), Expect = 1.6e-16
Identity = 49/128 (38.28%), Postives = 79/128 (61.72%), Query Frame = 0

Query: 499 TKKSDVYSFGIVLFELVSGQPAIIKGSEYNIHIVDWAKPLIAEGNIQNMVDPRLEGSIET 558
           T+KSDV+SFG+VL ELV+ QP I    E + HI +W   +++ G+I ++VDP+L+G  + 
Sbjct: 765 TEKSDVFSFGVVLLELVTNQPVIDMKREKS-HIAEWVGLMLSRGDINSIVDPKLQGDFDP 824

Query: 559 CSAGKFVELALSCTLSTSAERPDMSDVVSQLIECL--KMARDRTPQMPPNNTENFSYNSI 618
            +  K VE A++C   +S+ RP M+ VV  L ECL  +MAR+   +M  +  ++    S+
Sbjct: 825 NTIWKVVETAMTCLNPSSSRRPTMTQVVMDLKECLNMEMARNMGSRMTDSTNDSSIELSM 884

Query: 619 GSESILSP 625
              + L+P
Sbjct: 885 NFTTELNP 891

BLAST of Sgr025268 vs. ExPASy Swiss-Prot
Match: C0LGD6 (Probable LRR receptor-like serine/threonine-protein kinase At1g05700 OS=Arabidopsis thaliana OX=3702 GN=At1g05700 PE=1 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 3.4e-115
Identity = 232/492 (47.15%), Postives = 317/492 (64.43%), Query Frame = 0

Query: 12  FGFLLTGLPLALLLVVQ--AQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVS 71
           F +L+     AL LVV   AQDQSGFIS+DCG P G+SY + TT I+YVSD+S++ +GVS
Sbjct: 6   FLYLIYSAAFALCLVVSVLAQDQSGFISIDCGIPSGSSYKDDTTGINYVSDSSFVETGVS 65

Query: 72  ENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVV--KGTKYLIRASFLYGNYDGLNSLPK 131
           +++  T     QRQ+  LRSFP+G RNCY +  +  KG KYLIRASF+YGNYDG N  P+
Sbjct: 66  KSIPFT----AQRQLQNLRSFPEGSRNCYTLIPIQGKGKKYLIRASFMYGNYDGENGSPE 125

Query: 132 FDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPL--L 191
           FDL++G ++W+TV +++    V K++++++ S  + +CL N G G PFIS LE R L   
Sbjct: 126 FDLFLGGNIWDTVLLSNGSSIVSKEVVYLSQSENIFVCLGNKGKGTPFISTLELRFLGND 185

Query: 192 NITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGP 251
           N TY + +G+L    R D+ S      R+  DVYDRIW   NF    +++T   V  S  
Sbjct: 186 NTTYDSPNGALFFSRRWDLRSLMGSPVRYDDDVYDRIWIPRNFGYCREINTSLPV-TSDN 245

Query: 252 NNHRLPSIVMKTASAPTNASNPVEFWWDTLDSS-QYYVFMHFAEV--VKLQPNQSRGFNI 311
           N++ L S+VM TA  P N + P+    +  D + +Y+V+MHFAEV  + L+PNQ+R F+I
Sbjct: 246 NSYSLSSLVMSTAMTPINTTRPITMTLENSDPNVRYFVYMHFAEVEDLSLKPNQTREFDI 305

Query: 312 THNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNI 371
           + NG        P YL T + F  NP ++ ++  FS +    +TLPPI+NALEIY   + 
Sbjct: 306 SINGVTVAAGFSPKYLQT-NTFFLNP-ESQSKIAFSLVRTPKSTLPPIVNALEIYVANSF 365

Query: 372 SEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNE--TAPRIISLNLSA 431
           S+   +Q D DA+T +K++Y VK++W GDPC+P  Y W GL CS +  T PRI SLNLS+
Sbjct: 366 SQSLTNQEDGDAVTSLKTSYKVKKNWHGDPCLPNDYIWEGLNCSYDSLTPPRITSLNLSS 425

Query: 432 SGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALL 491
           SGL G ISS  SNLTM+Q LDLS N LTG++PEFL     LR LNL  N  TGSVP  LL
Sbjct: 426 SGLTGHISSSFSNLTMIQELDLSNNGLTGDIPEFLSKLKFLRVLNLENNTLTGSVPSELL 485

Query: 492 QRAEVGSLTLRI 493
           +R+  GS +LR+
Sbjct: 486 ERSNTGSFSLRL 490


HSP 2 Score: 75.1 bits (183), Expect = 3.1e-12
Identity = 44/140 (31.43%), Postives = 71/140 (50.71%), Query Frame = 0

Query: 457 NFPNLRNLNLSRNKFTGSVPK-ALLQRAEVGSLTLRIHLSGNFTKKSDVYSFGIVLFELV 516
           N   L +  LSR+  T S    + L     G L      +    +KSD+YSFG+VL E++
Sbjct: 705 NRAKLADFGLSRSFHTESRSHVSTLVAGTPGYLDPLCFETNGLNEKSDIYSFGVVLLEMI 764

Query: 517 SGQPAIIKGSEYNIHIVDWAKPLIAEGN-IQNMVDPRLEGSIETCSAGKFVELALSCTLS 576
           +G+  I +     +H+ DW   ++   N + N++D ++    +  S  K VELALS    
Sbjct: 765 TGKTVIKESQTKRVHVSDWVISILRSTNDVNNVIDSKMAKDFDVNSVWKVVELALSSVSQ 824

Query: 577 TSAERPDMSDVVSQLIECLK 595
             ++RP+M  +V  L ECL+
Sbjct: 825 NVSDRPNMPHIVRGLNECLQ 844

BLAST of Sgr025268 vs. ExPASy Swiss-Prot
Match: C0LGG4 (Probable LRR receptor-like serine/threonine-protein kinase At1g51860 OS=Arabidopsis thaliana OX=3702 GN=At1g51860 PE=2 SV=2)

HSP 1 Score: 403.3 bits (1035), Expect = 5.0e-111
Identity = 221/496 (44.56%), Postives = 312/496 (62.90%), Query Frame = 0

Query: 19  LPLALLLVVQAQDQSGFISLDCG-SPEGTSYPETTTNISYVSDASYINSGVSENVTSTYR 78
           +   +L  V+AQ+Q+GFISLDCG  P+ T+Y E +TNI+Y SD  YI+SG+   +   Y+
Sbjct: 13  IAFTVLRSVEAQNQAGFISLDCGLVPKETTYTEKSTNITYKSDVDYIDSGLVGKINDAYK 72

Query: 79  DSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLW 138
              Q+Q+  +RSFP G RNCYNV++    KYLIR +F+YGNYDGLN  P FDL++G + W
Sbjct: 73  TQFQQQVWAVRSFPVGQRNCYNVNLTANNKYLIRGTFVYGNYDGLNQFPSFDLHIGPNKW 132

Query: 139 ETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLS 198
            +V I         ++IH+   + +++CL+ TG   PFIS+LE RPL N +Y T SGSL 
Sbjct: 133 SSVKILGVTNTSMHEIIHVVPQDSLEVCLVKTGPTTPFISSLEVRPLNNESYLTQSGSLM 192

Query: 199 LHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKT 258
           L AR+   S+     R+  D++DR+W+++   E   +ST   +D S  N++ +P  VMKT
Sbjct: 193 LFARVYFPSSSSSFIRYDEDIHDRVWNSFTDDETVWISTDLPIDTS--NSYDMPQSVMKT 252

Query: 259 ASAPTNASNPVEFWWDTLD--SSQYYVFMHFAEVVKLQPNQSRGFNITHNGDL-FYGPLV 318
           A+ P NAS P   WW TLD  ++Q YV+MHFAEV  L  N++R FNIT+NG L ++  L 
Sbjct: 253 AAVPKNASEPWLLWW-TLDENTAQSYVYMHFAEVQNLTANETREFNITYNGGLRWFSYLR 312

Query: 319 PNYLSTLSVFSENPLDAANRHL-FSFIPIKNATLPPIINALEIYTVKNISEFEADQGDVD 378
           P  LS  ++F+   + ++N    F+F    N+TLPP++NALEIYTV +I + E ++ +V 
Sbjct: 313 PPNLSISTIFNPRAVSSSNGIFNFTFAMTGNSTLPPLLNALEIYTVVDILQLETNKDEVS 372

Query: 379 AITKIKSTYGV--KRDWQGDPCVPMGYPWSGLICS--NETAPRIISLNLSASGLIGEISS 438
           A+  IK TYG+  K  WQGDPC P  Y W GL CS  +    RIISLNL+ S L G I+S
Sbjct: 373 AMMNIKETYGLSKKISWQGDPCAPQLYRWEGLNCSYPDSEGSRIISLNLNGSELTGSITS 432

Query: 439 DISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTG--SVPKALLQRAEVGS 498
           DIS LT+L  LDLS N+L+G++P F     +L+ +NLS N      ++P +L QR    S
Sbjct: 433 DISKLTLLTVLDLSNNDLSGDIPTFFAEMKSLKLINLSGNPNLNLTAIPDSLQQRVNSKS 492

Query: 499 LTLRIHLSGNFTKKSD 504
           LTL +  +   T K +
Sbjct: 493 LTLILGENLTLTPKKE 505


HSP 2 Score: 90.5 bits (223), Expect = 7.2e-17
Identity = 46/101 (45.54%), Postives = 67/101 (66.34%), Query Frame = 0

Query: 499 TKKSDVYSFGIVLFELVSGQPAIIKGSEYNIHIVDWAKPLIAEGNIQNMVDPRLEGSIET 558
           ++KSDVYSFG+VL E+V+ QP I K  E   HI DW   ++ +G+I+++VDP+L G  +T
Sbjct: 763 SEKSDVYSFGVVLLEIVTNQPVIDKTRE-RPHINDWVGFMLTKGDIKSIVDPKLMGDYDT 822

Query: 559 CSAGKFVELALSCTLSTSAERPDMSDVVSQLIECLKMARDR 600
             A K VELAL+C   +S  RP M+ VV +L +C+ +   R
Sbjct: 823 NGAWKIVELALACVNPSSNRRPTMAHVVMELNDCVALENAR 862

BLAST of Sgr025268 vs. ExPASy Swiss-Prot
Match: O65924 (Putative leucine-rich repeat receptor-like protein kinase At2g19210 OS=Arabidopsis thaliana OX=3702 GN=At2g19210 PE=1 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 2.5e-110
Identity = 211/476 (44.33%), Postives = 297/476 (62.39%), Query Frame = 0

Query: 24  LLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENVTSTYRDSH-QR 83
           +L+V+AQDQSGF+S+DCG PE +SY + TT+I YVSDA+++ SG   ++   ++ S  ++
Sbjct: 20  VLLVRAQDQSGFVSIDCGIPEDSSYNDETTDIKYVSDAAFVESGTIHSIDPEFQTSSLEK 79

Query: 84  QMLKLRSFPQGIRNCYNVSVV--KGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLWETV 143
           Q   +RSFP+G RNCY+V     KG KYLIR  F+YGNYD L   P FDLY+G ++W++V
Sbjct: 80  QFQNVRSFPEGNRNCYDVKPPQGKGFKYLIRTRFMYGNYDNLGKAPDFDLYLGFNIWDSV 139

Query: 144 NITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLSLHA 203
            I +    V K++IH   S+ V +CL++   G PF+SALE R L + TY+T   SL L  
Sbjct: 140 TIDNATTIVTKEIIHTLRSDHVHVCLVDKNRGTPFLSALEIRLLKSNTYETPYDSLILFK 199

Query: 204 RLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKTASA 263
           R D+G       R+  DV+DRIW    F ++T  +    +D +     +    VM TA++
Sbjct: 200 RWDLGGLGALPVRYKDDVFDRIWIPLRFPKYTIFNASLTIDSNNNEGFQPARFVMNTATS 259

Query: 264 PTNASNPVEFWWDTLDSS-QYYVFMHFAEVVKLQPNQSRGFNITHN-GDLFYGPLVPNYL 323
           P + S  + F W+  D + +Y+V+MHFAEVV+L  N++R F +  N  ++      P YL
Sbjct: 260 PEDLSQDIIFSWEPKDPTWKYFVYMHFAEVVELPSNETREFKVLLNEKEINMSSFSPRYL 319

Query: 324 STLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNISEFEADQGDVDAITKI 383
            T ++F +NP+       F       +TLPPIINA+E Y V    +   DQ DVDAI +I
Sbjct: 320 YTDTLFVQNPVSGPKLE-FRLQQTPRSTLPPIINAIETYRVNEFLQSPTDQQDVDAIMRI 379

Query: 384 KSTYGVKRDWQGDPCVPMGYPWSGLICS--NETAPRIISLNLSASGLIGEISSDISNLTM 443
           KS YGVK+ W GDPC P+ YPW  + CS  +  +PRIIS+NLS+SGL GEI +  SNLT+
Sbjct: 380 KSKYGVKKSWLGDPCAPVKYPWKDINCSYVDNESPRIISVNLSSSGLTGEIDAAFSNLTL 439

Query: 444 LQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEVGSLTLRI 493
           L  LDLS N LTG++P+FL N  NL  LNL  NK +G++P  LL+R+    + LRI
Sbjct: 440 LHILDLSNNSLTGKIPDFLGNLHNLTELNLEGNKLSGAIPVKLLERSNKKLILLRI 494


HSP 2 Score: 95.5 bits (236), Expect = 2.3e-18
Identity = 49/115 (42.61%), Postives = 73/115 (63.48%), Query Frame = 0

Query: 485 VGSLTLRIHLSGNFTKKSDVYSFGIVLFELVSGQPAIIKG--SEYNIHIVDWAKPLIAEG 544
           +G L    HL+   ++KSD+YSFG+VL E+VSGQP I +   +  NIHI D    +++ G
Sbjct: 740 IGYLDPEYHLTQKLSEKSDIYSFGVVLLEVVSGQPVIARSRTTAENIHITDRVDLMLSTG 799

Query: 545 NIQNMVDPRLEGSIETCSAGKFVELALSCTLSTSAERPDMSDVVSQLIECLKMAR 598
           +I+ +VDP+L    +  SA K  E+A++C  S+S  RP MS VV++L E +  AR
Sbjct: 800 DIRGIVDPKLGERFDAGSAWKITEVAMACASSSSKNRPTMSHVVAELKESVSRAR 854

BLAST of Sgr025268 vs. ExPASy Swiss-Prot
Match: O64556 (Putative leucine-rich repeat receptor-like serine/threonine-protein kinase At2g19230 OS=Arabidopsis thaliana OX=3702 GN=At2g19230 PE=1 SV=3)

HSP 1 Score: 396.4 bits (1017), Expect = 6.2e-109
Identity = 208/481 (43.24%), Postives = 303/481 (62.99%), Query Frame = 0

Query: 21  LALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENVTSTYRDSH 80
           + +L++V AQDQSGF+S+DCG PE +SY +  T+I Y+SDA+++ SG   ++ S ++  +
Sbjct: 16  VVVLVLVCAQDQSGFVSIDCGIPEDSSYYDEKTDIKYISDAAFVESGTIHSIDSKFQKKN 75

Query: 81  -QRQMLKLRSFPQGIRNCYNVSVV--KGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLW 140
            ++Q  K+RSFP+G +NCY+V     KG KYLIR  F+YGNYD L   P FDLY+G +LW
Sbjct: 76  LEKQFQKVRSFPEGKKNCYDVQPPQGKGFKYLIRTRFMYGNYDNLGKAPDFDLYLGVNLW 135

Query: 141 ETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLS 200
           ++V + +    V K++I+   S++V +CL++   G PF+S LE R L N  Y+T S SL 
Sbjct: 136 DSVTLENSTTIVTKEIIYTLRSDKVHVCLVDKERGTPFLSVLELRLLKNNIYETASDSLM 195

Query: 201 LHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKT 260
           L+ R D+G+T     R+  D++DR W    F  +  ++T   +D +  N    PS+VM T
Sbjct: 196 LYRRWDLGATGDLPARYKDDIFDRFWMPLMFPNFLILNTSLMIDPTSSNGFLPPSVVMST 255

Query: 261 ASAPTNAS-NPVEFWWDTLDSS-QYYVFMHFAEVVKLQPNQSRGFNITHNGDLFYGPLV- 320
           A AP N+S   +  +W+  D + ++Y+++HFAEV KL  N++R F++  N +      V 
Sbjct: 256 AVAPMNSSIEQIMVYWEPRDPNWKFYIYIHFAEVEKLPSNETREFSVFLNKEQIDTTSVF 315

Query: 321 -PNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNISEFEADQGDVD 380
            P+YL T +++ +NP+            +K +T PPI+NA+E Y      +   DQ DVD
Sbjct: 316 RPSYLYTDTLYVQNPVSGPFLEFVLRQGVK-STRPPIMNAIETYRTNEFLDLPTDQNDVD 375

Query: 381 AITKIKSTYGVKRDWQGDPCVPMGYPWSGLICS--NETAPRIISLNLSASGLIGEISSDI 440
           AI KIK+ Y VK++W GDPC P GYPW G+ CS      PRIIS+NLS SGL G+I    
Sbjct: 376 AIMKIKTKYKVKKNWLGDPCAPFGYPWQGINCSYTANNPPRIISVNLSFSGLTGQIDPVF 435

Query: 441 SNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEVGSLTLR 493
             LT LQ LDLS N LTG +P+FL N P+L  LNL  NK TG +P+ LL+R++ GSL+LR
Sbjct: 436 ITLTPLQKLDLSNNRLTGTVPDFLANLPDLTELNLEENKLTGILPEKLLERSKDGSLSLR 495


HSP 2 Score: 82.0 bits (201), Expect = 2.6e-14
Identity = 45/114 (39.47%), Postives = 70/114 (61.40%), Query Frame = 0

Query: 485 VGSLTLRIHLSGNFTKKSDVYSFGIVLFELVSGQPAIIKG-SEYNIHIVDWAKPLIAEGN 544
           +G L    +    F++KSDVYSFG+VL E+++GQP I +  +E N HI D    ++++G+
Sbjct: 733 IGYLDPEHYSMQQFSEKSDVYSFGVVLLEVITGQPVISRSRTEENRHISDRVSLMLSKGD 792

Query: 545 IQNMVDPRLEGSIETCSAGKFVELALSCTLSTSAERPDMSDVVSQLIECLKMAR 598
           I+++VDP+L        A K  E+AL+C   ++  R  MS VV++L E L  AR
Sbjct: 793 IKSIVDPKLGERFNAGLAWKITEVALACASESTKTRLTMSQVVAELKESLCRAR 846

BLAST of Sgr025268 vs. ExPASy TrEMBL
Match: A0A6J1DFY8 (LRR receptor-like serine/threonine-protein kinase IOS1 OS=Momordica charantia OX=3673 GN=LOC111020154 PE=4 SV=1)

HSP 1 Score: 986.9 bits (2550), Expect = 3.9e-284
Identity = 538/879 (61.21%), Postives = 571/879 (64.96%), Query Frame = 0

Query: 2   MIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDA 61
           MIM T GH+LFGFLL      +LL+VQAQDQS FISLDCG PEGTSY ET+TNISY+SDA
Sbjct: 1   MIMETPGHSLFGFLL------ILLLVQAQDQSDFISLDCGLPEGTSYTETSTNISYISDA 60

Query: 62  SYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDG 121
           SYINSGVSENVT TYRDSHQRQMLKLRSFPQGIRNCYNVS+VKGTKYLIRASFLYGNYDG
Sbjct: 61  SYINSGVSENVTWTYRDSHQRQMLKLRSFPQGIRNCYNVSIVKGTKYLIRASFLYGNYDG 120

Query: 122 LNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEF 181
           LN LP FDLYVGNSLWETVNITD+GID+ KDLIHITS NEV ICLI+TGNGVPFISALEF
Sbjct: 121 LNLLPTFDLYVGNSLWETVNITDKGIDIVKDLIHITSLNEVHICLIDTGNGVPFISALEF 180

Query: 182 RPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVD 241
           RPLLN+TYQTVSGSLSL ARLD+GSTDY+NFRFPFDVYDRIW AYNF+ WTQVSTKH VD
Sbjct: 181 RPLLNMTYQTVSGSLSLAARLDVGSTDYQNFRFPFDVYDRIWPAYNFEGWTQVSTKHTVD 240

Query: 242 DSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRGFN 301
            SG NNH+LPSIVMKTAS P N S P EFWWDT+DSSQYYVFMHFAEVV+LQPNQ R FN
Sbjct: 241 PSG-NNHQLPSIVMKTASTPINGSKPAEFWWDTIDSSQYYVFMHFAEVVELQPNQIRAFN 300

Query: 302 ITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKN 361
           ITHNGDL++GP+VPNYLSTLSVFSE PL+AANRHLFSFI IKN TLPPI+NALEIYT KN
Sbjct: 301 ITHNGDLYFGPIVPNYLSTLSVFSEKPLEAANRHLFSFIAIKNTTLPPIVNALEIYTAKN 360

Query: 362 ISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLSAS 421
           ISEFEADQGDVDAIT IKSTYGVKRDWQGDPCVP+ YPWSGL CSNET PRIISLNLSAS
Sbjct: 361 ISEFEADQGDVDAITNIKSTYGVKRDWQGDPCVPIRYPWSGLTCSNETTPRIISLNLSAS 420

Query: 422 GLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQ 481
            LIGEISS ISNLTMLQTLDLSYNELTGELPEFLVNFPNLRN+NL+RNKFTGSVPKALLQ
Sbjct: 421 ELIGEISSYISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNINLTRNKFTGSVPKALLQ 480

Query: 482 RAEVGSLTL--------------------------------------------------- 541
           RAE GSLTL                                                   
Sbjct: 481 RAEAGSLTLSVGENPDLCTSLKCDNKRKNNKKKKHLLLIILSTIISVILPILMGILVIYK 540

Query: 542 ------------------------------------------------------------ 601
                                                                       
Sbjct: 541 RRKQRENLRRSIEERLLKSKNRQVRYSEILFISDNLKTVIGEGGFGKVYLGAVSDRTQVA 600

Query: 602 ------------------------------------------------------------ 626
                                                                       
Sbjct: 601 IKLLSKASRQGYNEFRAEAQILTVVHHKNLVALIGYCDEAENKALIYELMANGNLRKHLS 660

BLAST of Sgr025268 vs. ExPASy TrEMBL
Match: A0A6J1DP90 (probable LRR receptor-like serine/threonine-protein kinase At1g05700 OS=Momordica charantia OX=3673 GN=LOC111022244 PE=3 SV=1)

HSP 1 Score: 878.2 bits (2268), Expect = 2.0e-251
Identity = 481/881 (54.60%), Postives = 547/881 (62.09%), Query Frame = 0

Query: 2   MIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDA 61
           MI+GTSGH+LFGFLLTGL L LLL+VQAQDQSGFISLDCG PEGT Y ETTT I+YVSDA
Sbjct: 1   MIVGTSGHSLFGFLLTGLSLPLLLLVQAQDQSGFISLDCGLPEGTRYNETTTGINYVSDA 60

Query: 62  SYINSGVSENVTSTY--RDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNY 121
           S+INSGVS++V S Y   D++ RQ+  LRSFPQGIRNCYNVS+VKGT+YLIRASFLYGNY
Sbjct: 61  SFINSGVSKSVASAYGEGDTYPRQLRMLRSFPQGIRNCYNVSIVKGTEYLIRASFLYGNY 120

Query: 122 DGLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISAL 181
           DGL+SLP FDLY+GNSLW+T+  T   +D F DLIH+TSSNEV ICLINTGNGVPFISAL
Sbjct: 121 DGLDSLPMFDLYIGNSLWQTLKFTSNAMDAFIDLIHVTSSNEVHICLINTGNGVPFISAL 180

Query: 182 EFRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHA 241
           EFR  LNIT+QTV  SLSL+ R+DIGS + RN+RFP DVYDRIWS+YN  EWTQVST   
Sbjct: 181 EFRASLNITFQTVVTSLSLYTRMDIGSIEDRNYRFPLDVYDRIWSSYNSNEWTQVSTNLT 240

Query: 242 VDDSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRG 301
           VD  G N  RLPSIVM+TAS P NAS P+E WWD++DSSQYYVFMHFAE VK + NQ+R 
Sbjct: 241 VDALGGNRLRLPSIVMETASTPKNASKPLEIWWDSVDSSQYYVFMHFAEFVKPRANQTRE 300

Query: 302 FNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTV 361
           FNIT+NGD FYGP++PNYLS++++ +++PL+AANR+LFSF P +N+TLPPIINA EIYTV
Sbjct: 301 FNITYNGDFFYGPVIPNYLSSMTISTQDPLEAANRYLFSFTPTENSTLPPIINAFEIYTV 360

Query: 362 KNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLS 421
           K++SE +A+QGDVDAIT IKS+YG+KRDWQGDPCVPM YPWSGL CSN+TAPRIISLNLS
Sbjct: 361 KHVSELKANQGDVDAITNIKSSYGIKRDWQGDPCVPMRYPWSGLNCSNQTAPRIISLNLS 420

Query: 422 ASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKAL 481
           +SGL GEISS ISNLTMLQTLDLS NELTGELPEFLVN PNLR +NL+RNKFTGS+PKAL
Sbjct: 421 SSGLTGEISSYISNLTMLQTLDLSNNELTGELPEFLVNLPNLRIINLTRNKFTGSIPKAL 480

Query: 482 LQRAEVGSLTL------------------------------------------------- 541
           LQRAE GSLTL                                                 
Sbjct: 481 LQRAEAGSLTLSVGENPDLCTALKCDNKRNNNKKKKHLVLIILSCIIAVILPILMVSLVI 540

Query: 542 ------------------------------------------------------------ 601
                                                                       
Sbjct: 541 YKRRKQRENLKISVQERLLKSKNQQVHYSEILIITENLKTTIGEGGFGKVYLGVLSDKTQ 600

Query: 602 ------------------------------------------------------------ 626
                                                                       
Sbjct: 601 VAVKLLSAMSQQGYNEFRAEAQILTVVHHRNLVSLIGYCDEAENKALIYEFMGNGNLRDH 660

BLAST of Sgr025268 vs. ExPASy TrEMBL
Match: A0A6J1CSB5 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013870 PE=4 SV=1)

HSP 1 Score: 860.9 bits (2223), Expect = 3.3e-246
Identity = 474/835 (56.77%), Postives = 532/835 (63.71%), Query Frame = 0

Query: 1   MMIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSD 60
           MMIMG+SGH+LF F      L+L+L+VQAQDQSGFISLDCGSPEGTSY E TTNI+Y+SD
Sbjct: 1   MMIMGSSGHSLFAF------LSLILLVQAQDQSGFISLDCGSPEGTSYTEMTTNINYISD 60

Query: 61  ASYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYD 120
           A YINSGVSE+V   Y D++ RQ+  LRSFPQG+RNCYNVS+VKGTKYLIRASFLYGNYD
Sbjct: 61  AFYINSGVSESVALAYGDTNPRQLRNLRSFPQGVRNCYNVSIVKGTKYLIRASFLYGNYD 120

Query: 121 GLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALE 180
           GL+ LPKFDLYVG+SLWETVNITD+G+DVFKDLIH+TS NEV +CLINTGNGVPFISALE
Sbjct: 121 GLDLLPKFDLYVGSSLWETVNITDKGMDVFKDLIHMTSLNEVHMCLINTGNGVPFISALE 180

Query: 181 FRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAV 240
           FRPLLNITYQ  SGSLSL +R+DIGSTD R +RF FDVYDRIWS++     T+ ST   V
Sbjct: 181 FRPLLNITYQRKSGSLSLFSRMDIGSTDNRAYRFSFDVYDRIWSSFEDNVLTRESTNLTV 240

Query: 241 DDSGPNNHRLPSIVMKTASAPTNAS-NPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRG 300
           D    + +++PS+VMKTA  P       +EF W+T+DSSQYYVF+HFAE+VKLQPNQSR 
Sbjct: 241 DAIRRDGYQMPSVVMKTAYTPMGIGWEAMEFGWETVDSSQYYVFLHFAEIVKLQPNQSRV 300

Query: 301 FNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTV 360
           FNITHNG+ FYGPL+P+YLST +V SENPLDAA+ H+FSFIPI+NATLPPIINALEIY+V
Sbjct: 301 FNITHNGNHFYGPLIPSYLSTQTVSSENPLDAADSHIFSFIPIENATLPPIINALEIYSV 360

Query: 361 KNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLS 420
           K+ISE EA+QGDVDAIT IKSTY + RDWQGDPCVPMGYPWSGL CSNETAPRIISLNLS
Sbjct: 361 KDISELEANQGDVDAITNIKSTYAINRDWQGDPCVPMGYPWSGLHCSNETAPRIISLNLS 420

Query: 421 ASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKAL 480
           AS L GEISS ISNL MLQTLDLS NELTGELPEFLVN PNLR LNL++NKFTGSVPKAL
Sbjct: 421 ASELTGEISSYISNLAMLQTLDLSDNELTGELPEFLVNLPNLRTLNLTKNKFTGSVPKAL 480

Query: 481 LQRAEVGSLTL------------------------------------------------- 540
           LQRAE GSLTL                                                 
Sbjct: 481 LQRAEAGSLTLSADENPDLCTSLKCHNKWKKYLPLMILSTIIALLLPILMVTLIFYKKRK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 QRENLKRSVEERFLKSKNRQIQYSEILAITDNLKTTIGEGGFGKAQILTIAHHKNLVSLI 600

Query: 601 ------------------------------------------------------------ 616
                                                                       
Sbjct: 601 GYCDEAENKALVYEFMGNGNLRNHLLDSSTKVLSWMERLQIAVDAAQGLEYLHNGCKPPI 660

BLAST of Sgr025268 vs. ExPASy TrEMBL
Match: A0A6J1CSQ6 (LRR receptor-like serine/threonine-protein kinase IOS1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013870 PE=4 SV=1)

HSP 1 Score: 849.0 bits (2192), Expect = 1.3e-242
Identity = 474/866 (54.73%), Postives = 532/866 (61.43%), Query Frame = 0

Query: 1   MMIMGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSD 60
           MMIMG+SGH+LF F      L+L+L+VQAQDQSGFISLDCGSPEGTSY E TTNI+Y+SD
Sbjct: 1   MMIMGSSGHSLFAF------LSLILLVQAQDQSGFISLDCGSPEGTSYTEMTTNINYISD 60

Query: 61  ASYINSGVSENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYD 120
           A YINSGVSE+V   Y D++ RQ+  LRSFPQG+RNCYNVS+VKGTKYLIRASFLYGNYD
Sbjct: 61  AFYINSGVSESVALAYGDTNPRQLRNLRSFPQGVRNCYNVSIVKGTKYLIRASFLYGNYD 120

Query: 121 GLNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALE 180
           GL+ LPKFDLYVG+SLWETVNITD+G+DVFKDLIH+TS NEV +CLINTGNGVPFISALE
Sbjct: 121 GLDLLPKFDLYVGSSLWETVNITDKGMDVFKDLIHMTSLNEVHMCLINTGNGVPFISALE 180

Query: 181 FRPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAV 240
           FRPLLNITYQ  SGSLSL +R+DIGSTD R +RF FDVYDRIWS++     T+ ST   V
Sbjct: 181 FRPLLNITYQRKSGSLSLFSRMDIGSTDNRAYRFSFDVYDRIWSSFEDNVLTRESTNLTV 240

Query: 241 DDSGPNNHRLPSIVMKTASAPTNAS-NPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRG 300
           D    + +++PS+VMKTA  P       +EF W+T+DSSQYYVF+HFAE+VKLQPNQSR 
Sbjct: 241 DAIRRDGYQMPSVVMKTAYTPMGIGWEAMEFGWETVDSSQYYVFLHFAEIVKLQPNQSRV 300

Query: 301 FNITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTV 360
           FNITHNG+ FYGPL+P+YLST +V SENPLDAA+ H+FSFIPI+NATLPPIINALEIY+V
Sbjct: 301 FNITHNGNHFYGPLIPSYLSTQTVSSENPLDAADSHIFSFIPIENATLPPIINALEIYSV 360

Query: 361 KNISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLS 420
           K+ISE EA+QGDVDAIT IKSTY + RDWQGDPCVPMGYPWSGL CSNETAPRIISLNLS
Sbjct: 361 KDISELEANQGDVDAITNIKSTYAINRDWQGDPCVPMGYPWSGLHCSNETAPRIISLNLS 420

Query: 421 ASGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKAL 480
           AS L GEISS ISNL MLQTLDLS NELTGELPEFLVN PNLR LNL++NKFTGSVPKAL
Sbjct: 421 ASELTGEISSYISNLAMLQTLDLSDNELTGELPEFLVNLPNLRTLNLTKNKFTGSVPKAL 480

Query: 481 LQRAEVGSLTL------------------------------------------------- 540
           LQRAE GSLTL                                                 
Sbjct: 481 LQRAEAGSLTLSADENPDLCTSLKCHNKWKKYLPLMILSTIIALLLPILMVTLIFYKKRK 540

Query: 541 ------------------------------------------------------------ 600
                                                                       
Sbjct: 541 QRENLKRSVEERFLKSKNRQIQYSEILAITDNLKTTIGEGGFGKVYLGVLSDKTKVAVKL 600

Query: 601 ------------------------------------------------------------ 616
                                                                       
Sbjct: 601 LSGSSQQGSNEFKAEAQILTIAHHKNLVSLIGYCDEAENKALVYEFMGNGNLRNHLLDSS 660

BLAST of Sgr025268 vs. ExPASy TrEMBL
Match: A0A5D3BXW9 (Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold347G00480 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 5.4e-225
Identity = 445/882 (50.45%), Postives = 516/882 (58.50%), Query Frame = 0

Query: 4   MGTSGHTLFGFLLTGLPLALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASY 63
           MGTSGH LFGFLLT L LALLL VQAQDQSGFISLDCG PEGTSY ETTT ++YVSDAS+
Sbjct: 1   MGTSGHFLFGFLLTALALALLL-VQAQDQSGFISLDCGLPEGTSYTETTTKLNYVSDASF 60

Query: 64  INSGVSENVTSTY--RDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDG 123
           INSGVS++V S Y   D++ RQ+ KLRSFPQGIRNCY+V+ VKGT+YLIR SFLYGNYDG
Sbjct: 61  INSGVSQDVASAYGDGDTYPRQLRKLRSFPQGIRNCYSVTTVKGTEYLIRGSFLYGNYDG 120

Query: 124 LNSLPKFDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEF 183
           L+ LP FDLY+ NSLW+T+N TD G+D + DLIH+TSSN+V ICLINTGNGVPFISALEF
Sbjct: 121 LDLLPMFDLYIENSLWQTLNFTDNGMDAYIDLIHVTSSNKVNICLINTGNGVPFISALEF 180

Query: 184 RPLLNITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVD 243
           RP LNITY T++ SLSL+ R++IGST+ R +RFPFDVYDRIWS +NF +WTQVST ++++
Sbjct: 181 RPSLNITYLTIASSLSLYTRMNIGSTEDRKYRFPFDVYDRIWSPFNFNKWTQVSTNYSIE 240

Query: 244 DSGPNNHRLPSIVMKTASAPTNASNPVEFWWDTLDSSQYYVFMHFAEVVKLQPNQSRGFN 303
               N   LPSIVM+TAS   + S P+E WWD +DSSQYYVFMH AEV+  + NQSR F 
Sbjct: 241 PIRENGLHLPSIVMQTASTSKDTSTPLEIWWDPIDSSQYYVFMHLAEVLYPEVNQSREFI 300

Query: 304 ITHNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKN 363
           IT N +   GP++PNYLS++S+F   P + A+RH+ +FI  + ATLPPIINA E+Y VKN
Sbjct: 301 ITTNDNFVSGPIIPNYLSSVSIFPNGPFEGASRHVIAFISTEEATLPPIINAFELYIVKN 360

Query: 364 ISEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNETAPRIISLNLSAS 423
           IS+ EADQGDVDA+T IKSTYG+K++WQGDPCVPMG+PWSGL CSN T PRIISLNLSAS
Sbjct: 361 ISKLEADQGDVDALTNIKSTYGIKKEWQGDPCVPMGFPWSGLHCSNGTIPRIISLNLSAS 420

Query: 424 GLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQ 483
           GL GEIS  ISNLTMLQ LDLS NELTGELPEFLVN PNLR L L+RN+FTG +PKALLQ
Sbjct: 421 GLTGEISPYISNLTMLQILDLSDNELTGELPEFLVNLPNLRILYLTRNRFTGLIPKALLQ 480

Query: 484 RAEVGSLTL--------------------------------------------------- 543
           RAE G L L                                                   
Sbjct: 481 RAEAGLLALSVGENPNLCTSVECVKKRKNNKRKKYLVAIILSSIIAVLLTILMVTLVIYK 540

Query: 544 ------------------------------------------------------------ 603
                                                                       
Sbjct: 541 RRKQRENLKRSIQERLLKSKNQLVQYSEILVITDNLKTTIGEGGFGKVYLGVLSDKTRVA 600

Query: 604 ------------------------------------------------------------ 626
                                                                       
Sbjct: 601 VKLMSSTSQQGYNEFRAEAQILTVVHHINLVSLIGYCDEAENKALLYEFMGNGSLRDFLS 660

BLAST of Sgr025268 vs. TAIR 10
Match: AT1G51800.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 450.7 bits (1158), Expect = 2.0e-126
Identity = 241/487 (49.49%), Postives = 329/487 (67.56%), Query Frame = 0

Query: 14  FLLTGLPL-ALLLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENV 73
           FLL  L + + LL+  AQDQSGFISLDCGSP  TS+ E TTNI+Y+SDA++IN+GV  ++
Sbjct: 7   FLLVLLQIFSALLLCLAQDQSGFISLDCGSPRETSFREKTTNITYISDANFINTGVGGSI 66

Query: 74  TSTYRDSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDGLNSLPKFDLYV 133
              YR   Q+Q   LRSFPQGIRNCY +++  G +YLIRA+FL+G YD   S  +F+LY+
Sbjct: 67  KQGYRTQFQQQTWNLRSFPQGIRNCYTLNLTIGDEYLIRANFLHGGYDDKPS-TQFELYL 126

Query: 134 GNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTV 193
           G +LW TV  T+E      ++IHI +++ +QICL+ TGN  PFISALE R L+N TY T 
Sbjct: 127 GPNLWSTVTTTNETEASIFEMIHILTTDRLQICLVKTGNATPFISALELRKLMNTTYLTR 186

Query: 194 SGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPS 253
            GSL    R D+G+T  + +R+  DV+DR+W+ YNF  W+Q+ST  +V+ +  N+++ P 
Sbjct: 187 QGSLQTFIRADVGATVNQGYRYGIDVFDRVWTPYNFGNWSQISTNQSVNIN--NDYQPPE 246

Query: 254 IVMKTASAPTNASNPVEFWWDTLDSS-QYYVFMHFAEVVKLQPNQSRGFNITHNGDLFYG 313
           I M TAS PT+    +      ++ + Q+YVFMHFAE+ +L+ N +R FNI +N    YG
Sbjct: 247 IAMVTASVPTDPDAAMNISLVGVERTVQFYVFMHFAEIQELKSNDTREFNIMYNNKHIYG 306

Query: 314 PLVPNYLSTLSVF--SENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNISEFEADQ 373
           P  P   +T SVF  +E   DA  +++FS     N+TLPP++NA+EIY+V  + + E D+
Sbjct: 307 PFRPLNFTTSSVFTPTEVVADANGQYIFSLQRTGNSTLPPLLNAMEIYSVNLLPQQETDR 366

Query: 374 GDVDAITKIKSTYGV-KRDWQGDPCVPMGYPWSGLICS---NETAPRIISLNLSASGLIG 433
            +VDA+  IKS YGV K DW+GDPCVP+ Y WSG+ C+   NET P+IISL+LS SGL G
Sbjct: 367 KEVDAMMNIKSAYGVNKIDWEGDPCVPLDYKWSGVNCTYVDNET-PKIISLDLSTSGLTG 426

Query: 434 EISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEV 493
           EI   IS+LT L+ LDLS N LTG +PEFL N   L+ +NLS N+  GS+P  LL +   
Sbjct: 427 EILEFISDLTSLEVLDLSNNSLTGSVPEFLANMETLKLINLSGNELNGSIPATLLDKERR 486


HSP 2 Score: 89.4 bits (220), Expect = 1.1e-17
Identity = 49/128 (38.28%), Postives = 79/128 (61.72%), Query Frame = 0

Query: 499 TKKSDVYSFGIVLFELVSGQPAIIKGSEYNIHIVDWAKPLIAEGNIQNMVDPRLEGSIET 558
           T+KSDV+SFG+VL ELV+ QP I    E + HI +W   +++ G+I ++VDP+L+G  + 
Sbjct: 765 TEKSDVFSFGVVLLELVTNQPVIDMKREKS-HIAEWVGLMLSRGDINSIVDPKLQGDFDP 824

Query: 559 CSAGKFVELALSCTLSTSAERPDMSDVVSQLIECL--KMARDRTPQMPPNNTENFSYNSI 618
            +  K VE A++C   +S+ RP M+ VV  L ECL  +MAR+   +M  +  ++    S+
Sbjct: 825 NTIWKVVETAMTCLNPSSSRRPTMTQVVMDLKECLNMEMARNMGSRMTDSTNDSSIELSM 884

Query: 619 GSESILSP 625
              + L+P
Sbjct: 885 NFTTELNP 891

BLAST of Sgr025268 vs. TAIR 10
Match: AT1G05700.1 (Leucine-rich repeat transmembrane protein kinase protein )

HSP 1 Score: 417.2 bits (1071), Expect = 2.4e-116
Identity = 232/492 (47.15%), Postives = 317/492 (64.43%), Query Frame = 0

Query: 12  FGFLLTGLPLALLLVVQ--AQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVS 71
           F +L+     AL LVV   AQDQSGFIS+DCG P G+SY + TT I+YVSD+S++ +GVS
Sbjct: 6   FLYLIYSAAFALCLVVSVLAQDQSGFISIDCGIPSGSSYKDDTTGINYVSDSSFVETGVS 65

Query: 72  ENVTSTYRDSHQRQMLKLRSFPQGIRNCYNVSVV--KGTKYLIRASFLYGNYDGLNSLPK 131
           +++  T     QRQ+  LRSFP+G RNCY +  +  KG KYLIRASF+YGNYDG N  P+
Sbjct: 66  KSIPFT----AQRQLQNLRSFPEGSRNCYTLIPIQGKGKKYLIRASFMYGNYDGENGSPE 125

Query: 132 FDLYVGNSLWETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPL--L 191
           FDL++G ++W+TV +++    V K++++++ S  + +CL N G G PFIS LE R L   
Sbjct: 126 FDLFLGGNIWDTVLLSNGSSIVSKEVVYLSQSENIFVCLGNKGKGTPFISTLELRFLGND 185

Query: 192 NITYQTVSGSLSLHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGP 251
           N TY + +G+L    R D+ S      R+  DVYDRIW   NF    +++T   V  S  
Sbjct: 186 NTTYDSPNGALFFSRRWDLRSLMGSPVRYDDDVYDRIWIPRNFGYCREINTSLPV-TSDN 245

Query: 252 NNHRLPSIVMKTASAPTNASNPVEFWWDTLDSS-QYYVFMHFAEV--VKLQPNQSRGFNI 311
           N++ L S+VM TA  P N + P+    +  D + +Y+V+MHFAEV  + L+PNQ+R F+I
Sbjct: 246 NSYSLSSLVMSTAMTPINTTRPITMTLENSDPNVRYFVYMHFAEVEDLSLKPNQTREFDI 305

Query: 312 THNGDLFYGPLVPNYLSTLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNI 371
           + NG        P YL T + F  NP ++ ++  FS +    +TLPPI+NALEIY   + 
Sbjct: 306 SINGVTVAAGFSPKYLQT-NTFFLNP-ESQSKIAFSLVRTPKSTLPPIVNALEIYVANSF 365

Query: 372 SEFEADQGDVDAITKIKSTYGVKRDWQGDPCVPMGYPWSGLICSNE--TAPRIISLNLSA 431
           S+   +Q D DA+T +K++Y VK++W GDPC+P  Y W GL CS +  T PRI SLNLS+
Sbjct: 366 SQSLTNQEDGDAVTSLKTSYKVKKNWHGDPCLPNDYIWEGLNCSYDSLTPPRITSLNLSS 425

Query: 432 SGLIGEISSDISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALL 491
           SGL G ISS  SNLTM+Q LDLS N LTG++PEFL     LR LNL  N  TGSVP  LL
Sbjct: 426 SGLTGHISSSFSNLTMIQELDLSNNGLTGDIPEFLSKLKFLRVLNLENNTLTGSVPSELL 485

Query: 492 QRAEVGSLTLRI 493
           +R+  GS +LR+
Sbjct: 486 ERSNTGSFSLRL 490


HSP 2 Score: 75.1 bits (183), Expect = 2.2e-13
Identity = 44/140 (31.43%), Postives = 71/140 (50.71%), Query Frame = 0

Query: 457 NFPNLRNLNLSRNKFTGSVPK-ALLQRAEVGSLTLRIHLSGNFTKKSDVYSFGIVLFELV 516
           N   L +  LSR+  T S    + L     G L      +    +KSD+YSFG+VL E++
Sbjct: 705 NRAKLADFGLSRSFHTESRSHVSTLVAGTPGYLDPLCFETNGLNEKSDIYSFGVVLLEMI 764

Query: 517 SGQPAIIKGSEYNIHIVDWAKPLIAEGN-IQNMVDPRLEGSIETCSAGKFVELALSCTLS 576
           +G+  I +     +H+ DW   ++   N + N++D ++    +  S  K VELALS    
Sbjct: 765 TGKTVIKESQTKRVHVSDWVISILRSTNDVNNVIDSKMAKDFDVNSVWKVVELALSSVSQ 824

Query: 577 TSAERPDMSDVVSQLIECLK 595
             ++RP+M  +V  L ECL+
Sbjct: 825 NVSDRPNMPHIVRGLNECLQ 844

BLAST of Sgr025268 vs. TAIR 10
Match: AT4G29990.1 (Leucine-rich repeat transmembrane protein kinase protein )

HSP 1 Score: 404.4 bits (1038), Expect = 1.6e-112
Identity = 214/475 (45.05%), Postives = 300/475 (63.16%), Query Frame = 0

Query: 26  VVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENVTSTYR-DSHQRQM 85
           +V AQDQSGFIS+DCG P+ +SY +  TN+ YVSD  ++ SG S ++ S  +  S +RQ 
Sbjct: 18  LVFAQDQSGFISIDCGIPDDSSYTDEKTNMKYVSDLGFVESGTSHSIVSDLQTTSLERQF 77

Query: 86  LKLRSFPQGIRNCYNV--SVVKGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLWETVNI 145
             +RSFP+G RNCY++     KG KYLIR  F+YGNYDG +  P+FDLY+G +LWE+V +
Sbjct: 78  QNVRSFPEGKRNCYDIRPQQGKGFKYLIRTRFMYGNYDGFSKTPEFDLYIGANLWESVVL 137

Query: 146 TDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLSLHARL 205
            +E   + K++I+   S+ + +CL++   G PF+S LE R L N TY T   +L L  R 
Sbjct: 138 INETAIMTKEIIYTPPSDHIHVCLVDKNRGTPFLSVLEIRFLKNDTYDTPYEALMLGRRW 197

Query: 206 DIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKTASAPT 265
           D G+      R+  D YDRIW  Y       ++T   +D++  N  R  SIVM++A AP 
Sbjct: 198 DFGTATNLQIRYKDDFYDRIWMPYKSPYQKTLNTSLTIDETNHNGFRPASIVMRSAIAPG 257

Query: 266 NASNPVEFWWDTLD-SSQYYVFMHFAEVVKLQPNQSRGFNITHNGDLFYGPLVPNYLSTL 325
           N SNP++F W   D  S++Y++MHFAEV +LQ N++R F+I  N  +      P YL T 
Sbjct: 258 NESNPLKFNWAPDDPRSKFYIYMHFAEVRELQRNETREFDIYINDVILAENFRPFYLFT- 317

Query: 326 SVFSENPLDAANRHLFSFIPIKN--ATLPPIINALEIYTVKNISEFEADQGDVDAITKIK 385
              + + +D   R +   +  +   +TLPPIINA+EIY +    +   DQ DVDA+TKIK
Sbjct: 318 --DTRSTVDPVGRKMNEIVLQRTGVSTLPPIINAIEIYQINEFLQLPTDQQDVDAMTKIK 377

Query: 386 STYGVKRDWQGDPCVPMGYPWSGLIC---SNETAPRIISLNLSASGLIGEISSDISNLTM 445
             Y VK++WQGDPCVP+   W GL C    N T+P+ I+LNLS+SGL G+I    +NLT 
Sbjct: 378 FKYRVKKNWQGDPCVPVDNSWEGLECLHSDNNTSPKSIALNLSSSGLTGQIDPAFANLTS 437

Query: 446 LQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEVGSLTLR 492
           +  LDLS N LTG++P+FL + PNL  LNL  NK TGS+P  LL++++ GSL+LR
Sbjct: 438 INKLDLSNNSLTGKVPDFLASLPNLTELNLEGNKLTGSIPAKLLEKSKDGSLSLR 489


HSP 2 Score: 88.2 bits (217), Expect = 2.6e-17
Identity = 48/130 (36.92%), Postives = 74/130 (56.92%), Query Frame = 0

Query: 461 LRNLNLSRN-KFTGSVPKALLQRAEVGSLTLRIHLSGNFTKKSDVYSFGIVLFELVSGQP 520
           + +  LSR+    GS   + +    +G L    + +    +KSDVYSFG+VL E+++G+P
Sbjct: 713 IADFGLSRSFPVEGSSQVSTVVAGTIGYLDPEYYATRQMNEKSDVYSFGVVLLEVITGKP 772

Query: 521 AIIKGSEYNIHIVDWAKPLIAEGNIQNMVDPRLEGSIETCSAGKFVELALSCTLSTSAER 580
           AI      ++H+ D    ++A G+I+ +VD RL    E  SA K  ELAL+C   +S +R
Sbjct: 773 AIWHSRTESVHLSDQVGSMLANGDIKGIVDQRLGDRFEVGSAWKITELALACASESSEQR 832

Query: 581 PDMSDVVSQL 590
           P MS VV +L
Sbjct: 833 PTMSQVVMEL 842

BLAST of Sgr025268 vs. TAIR 10
Match: AT1G51860.1 (Leucine-rich repeat protein kinase family protein )

HSP 1 Score: 403.3 bits (1035), Expect = 3.6e-112
Identity = 221/496 (44.56%), Postives = 312/496 (62.90%), Query Frame = 0

Query: 19  LPLALLLVVQAQDQSGFISLDCG-SPEGTSYPETTTNISYVSDASYINSGVSENVTSTYR 78
           +   +L  V+AQ+Q+GFISLDCG  P+ T+Y E +TNI+Y SD  YI+SG+   +   Y+
Sbjct: 13  IAFTVLRSVEAQNQAGFISLDCGLVPKETTYTEKSTNITYKSDVDYIDSGLVGKINDAYK 72

Query: 79  DSHQRQMLKLRSFPQGIRNCYNVSVVKGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLW 138
              Q+Q+  +RSFP G RNCYNV++    KYLIR +F+YGNYDGLN  P FDL++G + W
Sbjct: 73  TQFQQQVWAVRSFPVGQRNCYNVNLTANNKYLIRGTFVYGNYDGLNQFPSFDLHIGPNKW 132

Query: 139 ETVNITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLS 198
            +V I         ++IH+   + +++CL+ TG   PFIS+LE RPL N +Y T SGSL 
Sbjct: 133 SSVKILGVTNTSMHEIIHVVPQDSLEVCLVKTGPTTPFISSLEVRPLNNESYLTQSGSLM 192

Query: 199 LHARLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKT 258
           L AR+   S+     R+  D++DR+W+++   E   +ST   +D S  N++ +P  VMKT
Sbjct: 193 LFARVYFPSSSSSFIRYDEDIHDRVWNSFTDDETVWISTDLPIDTS--NSYDMPQSVMKT 252

Query: 259 ASAPTNASNPVEFWWDTLD--SSQYYVFMHFAEVVKLQPNQSRGFNITHNGDL-FYGPLV 318
           A+ P NAS P   WW TLD  ++Q YV+MHFAEV  L  N++R FNIT+NG L ++  L 
Sbjct: 253 AAVPKNASEPWLLWW-TLDENTAQSYVYMHFAEVQNLTANETREFNITYNGGLRWFSYLR 312

Query: 319 PNYLSTLSVFSENPLDAANRHL-FSFIPIKNATLPPIINALEIYTVKNISEFEADQGDVD 378
           P  LS  ++F+   + ++N    F+F    N+TLPP++NALEIYTV +I + E ++ +V 
Sbjct: 313 PPNLSISTIFNPRAVSSSNGIFNFTFAMTGNSTLPPLLNALEIYTVVDILQLETNKDEVS 372

Query: 379 AITKIKSTYGV--KRDWQGDPCVPMGYPWSGLICS--NETAPRIISLNLSASGLIGEISS 438
           A+  IK TYG+  K  WQGDPC P  Y W GL CS  +    RIISLNL+ S L G I+S
Sbjct: 373 AMMNIKETYGLSKKISWQGDPCAPQLYRWEGLNCSYPDSEGSRIISLNLNGSELTGSITS 432

Query: 439 DISNLTMLQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTG--SVPKALLQRAEVGS 498
           DIS LT+L  LDLS N+L+G++P F     +L+ +NLS N      ++P +L QR    S
Sbjct: 433 DISKLTLLTVLDLSNNDLSGDIPTFFAEMKSLKLINLSGNPNLNLTAIPDSLQQRVNSKS 492

Query: 499 LTLRIHLSGNFTKKSD 504
           LTL +  +   T K +
Sbjct: 493 LTLILGENLTLTPKKE 505


HSP 2 Score: 90.5 bits (223), Expect = 5.1e-18
Identity = 46/101 (45.54%), Postives = 67/101 (66.34%), Query Frame = 0

Query: 499 TKKSDVYSFGIVLFELVSGQPAIIKGSEYNIHIVDWAKPLIAEGNIQNMVDPRLEGSIET 558
           ++KSDVYSFG+VL E+V+ QP I K  E   HI DW   ++ +G+I+++VDP+L G  +T
Sbjct: 763 SEKSDVYSFGVVLLEIVTNQPVIDKTRE-RPHINDWVGFMLTKGDIKSIVDPKLMGDYDT 822

Query: 559 CSAGKFVELALSCTLSTSAERPDMSDVVSQLIECLKMARDR 600
             A K VELAL+C   +S  RP M+ VV +L +C+ +   R
Sbjct: 823 NGAWKIVELALACVNPSSNRRPTMAHVVMELNDCVALENAR 862

BLAST of Sgr025268 vs. TAIR 10
Match: AT2G19210.1 (Leucine-rich repeat transmembrane protein kinase protein )

HSP 1 Score: 401.0 bits (1029), Expect = 1.8e-111
Identity = 211/476 (44.33%), Postives = 297/476 (62.39%), Query Frame = 0

Query: 24  LLVVQAQDQSGFISLDCGSPEGTSYPETTTNISYVSDASYINSGVSENVTSTYRDSH-QR 83
           +L+V+AQDQSGF+S+DCG PE +SY + TT+I YVSDA+++ SG   ++   ++ S  ++
Sbjct: 20  VLLVRAQDQSGFVSIDCGIPEDSSYNDETTDIKYVSDAAFVESGTIHSIDPEFQTSSLEK 79

Query: 84  QMLKLRSFPQGIRNCYNVSVV--KGTKYLIRASFLYGNYDGLNSLPKFDLYVGNSLWETV 143
           Q   +RSFP+G RNCY+V     KG KYLIR  F+YGNYD L   P FDLY+G ++W++V
Sbjct: 80  QFQNVRSFPEGNRNCYDVKPPQGKGFKYLIRTRFMYGNYDNLGKAPDFDLYLGFNIWDSV 139

Query: 144 NITDEGIDVFKDLIHITSSNEVQICLINTGNGVPFISALEFRPLLNITYQTVSGSLSLHA 203
            I +    V K++IH   S+ V +CL++   G PF+SALE R L + TY+T   SL L  
Sbjct: 140 TIDNATTIVTKEIIHTLRSDHVHVCLVDKNRGTPFLSALEIRLLKSNTYETPYDSLILFK 199

Query: 204 RLDIGSTDYRNFRFPFDVYDRIWSAYNFKEWTQVSTKHAVDDSGPNNHRLPSIVMKTASA 263
           R D+G       R+  DV+DRIW    F ++T  +    +D +     +    VM TA++
Sbjct: 200 RWDLGGLGALPVRYKDDVFDRIWIPLRFPKYTIFNASLTIDSNNNEGFQPARFVMNTATS 259

Query: 264 PTNASNPVEFWWDTLDSS-QYYVFMHFAEVVKLQPNQSRGFNITHN-GDLFYGPLVPNYL 323
           P + S  + F W+  D + +Y+V+MHFAEVV+L  N++R F +  N  ++      P YL
Sbjct: 260 PEDLSQDIIFSWEPKDPTWKYFVYMHFAEVVELPSNETREFKVLLNEKEINMSSFSPRYL 319

Query: 324 STLSVFSENPLDAANRHLFSFIPIKNATLPPIINALEIYTVKNISEFEADQGDVDAITKI 383
            T ++F +NP+       F       +TLPPIINA+E Y V    +   DQ DVDAI +I
Sbjct: 320 YTDTLFVQNPVSGPKLE-FRLQQTPRSTLPPIINAIETYRVNEFLQSPTDQQDVDAIMRI 379

Query: 384 KSTYGVKRDWQGDPCVPMGYPWSGLICS--NETAPRIISLNLSASGLIGEISSDISNLTM 443
           KS YGVK+ W GDPC P+ YPW  + CS  +  +PRIIS+NLS+SGL GEI +  SNLT+
Sbjct: 380 KSKYGVKKSWLGDPCAPVKYPWKDINCSYVDNESPRIISVNLSSSGLTGEIDAAFSNLTL 439

Query: 444 LQTLDLSYNELTGELPEFLVNFPNLRNLNLSRNKFTGSVPKALLQRAEVGSLTLRI 493
           L  LDLS N LTG++P+FL N  NL  LNL  NK +G++P  LL+R+    + LRI
Sbjct: 440 LHILDLSNNSLTGKIPDFLGNLHNLTELNLEGNKLSGAIPVKLLERSNKKLILLRI 494


HSP 2 Score: 95.5 bits (236), Expect = 1.6e-19
Identity = 49/115 (42.61%), Postives = 73/115 (63.48%), Query Frame = 0

Query: 485 VGSLTLRIHLSGNFTKKSDVYSFGIVLFELVSGQPAIIKG--SEYNIHIVDWAKPLIAEG 544
           +G L    HL+   ++KSD+YSFG+VL E+VSGQP I +   +  NIHI D    +++ G
Sbjct: 740 IGYLDPEYHLTQKLSEKSDIYSFGVVLLEVVSGQPVIARSRTTAENIHITDRVDLMLSTG 799

Query: 545 NIQNMVDPRLEGSIETCSAGKFVELALSCTLSTSAERPDMSDVVSQLIECLKMAR 598
           +I+ +VDP+L    +  SA K  E+A++C  S+S  RP MS VV++L E +  AR
Sbjct: 800 DIRGIVDPKLGERFDAGSAWKITEVAMACASSSSKNRPTMSHVVAELKESVSRAR 854

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152419.18.1e-28461.21LRR receptor-like serine/threonine-protein kinase IOS1 [Momordica charantia][more]
XP_022155109.14.1e-25154.60probable LRR receptor-like serine/threonine-protein kinase At1g05700 [Momordica ... [more]
XP_022144106.16.7e-24656.77LRR receptor-like serine/threonine-protein kinase IOS1 isoform X2 [Momordica cha... [more]
XP_022144097.12.7e-24254.73LRR receptor-like serine/threonine-protein kinase IOS1 isoform X1 [Momordica cha... [more]
XP_038896176.11.3e-22851.48putative leucine-rich repeat receptor-like serine/threonine-protein kinase At2g1... [more]
Match NameE-valueIdentityDescription
Q9C8I62.8e-12549.49LRR receptor-like serine/threonine-protein kinase IOS1 OS=Arabidopsis thaliana O... [more]
C0LGD63.4e-11547.15Probable LRR receptor-like serine/threonine-protein kinase At1g05700 OS=Arabidop... [more]
C0LGG45.0e-11144.56Probable LRR receptor-like serine/threonine-protein kinase At1g51860 OS=Arabidop... [more]
O659242.5e-11044.33Putative leucine-rich repeat receptor-like protein kinase At2g19210 OS=Arabidops... [more]
O645566.2e-10943.24Putative leucine-rich repeat receptor-like serine/threonine-protein kinase At2g1... [more]
Match NameE-valueIdentityDescription
A0A6J1DFY83.9e-28461.21LRR receptor-like serine/threonine-protein kinase IOS1 OS=Momordica charantia OX... [more]
A0A6J1DP902.0e-25154.60probable LRR receptor-like serine/threonine-protein kinase At1g05700 OS=Momordic... [more]
A0A6J1CSB53.3e-24656.77LRR receptor-like serine/threonine-protein kinase IOS1 isoform X2 OS=Momordica c... [more]
A0A6J1CSQ61.3e-24254.73LRR receptor-like serine/threonine-protein kinase IOS1 isoform X1 OS=Momordica c... [more]
A0A5D3BXW95.4e-22550.45Putative LRR receptor-like serine/threonine-protein kinase OS=Cucumis melo var. ... [more]
Match NameE-valueIdentityDescription
AT1G51800.12.0e-12649.49Leucine-rich repeat protein kinase family protein [more]
AT1G05700.12.4e-11647.15Leucine-rich repeat transmembrane protein kinase protein [more]
AT4G29990.11.6e-11245.05Leucine-rich repeat transmembrane protein kinase protein [more]
AT1G51860.13.6e-11244.56Leucine-rich repeat protein kinase family protein [more]
AT2G19210.11.8e-11144.33Leucine-rich repeat transmembrane protein kinase protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 372..483
e-value: 9.4E-27
score: 95.3
IPR024788Malectin-like domainPFAMPF12819Malectin_likecoord: 38..359
e-value: 9.5E-110
score: 366.9
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 496..589
e-value: 9.4E-6
score: 25.1
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 484..612
e-value: 3.2E-21
score: 77.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 599..625
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 601..625
NoneNo IPR availablePANTHERPTHR45631OS07G0107800 PROTEIN-RELATEDcoord: 21..493
NoneNo IPR availablePANTHERPTHR45631OS07G0107800 PROTEIN-RELATEDcoord: 497..612
NoneNo IPR availablePANTHERPTHR45631:SF105PUTATIVE-RELATEDcoord: 497..612
coord: 21..493
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 396..484
IPR025875Leucine rich repeat 4PFAMPF12799LRR_4coord: 435..472
e-value: 3.4E-7
score: 30.5
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 494..610

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr025268.1Sgr025268.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity