Sgr025151 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr025151
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Diacylglycerol O-acyltransferase
Location	tig00003412: 1738450 .. 1739734 (-)
RNA-Seq Expression	Sgr025151
Synteny	Sgr025151

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGATTTTCGAAGATTTAGTCCCAGTGAGTCCTTTGAGCCAATACTTCAACACCTCAGTGATGTGTGTTTCTGTTCTGGGCGTCATCGAACTTGAAACTTCAGTCTCTCCCTCCGATGCCTCATTAGTGATCACTGATAGTCTCCTCCCTGCCAACCCACGCTTCAGTTCAATCATGGTTCTCTCTCTCTCTCTCTCATTAGTACGTAAGTAAAACGTTGCATGCATGCATGCAGGTGAACGACAAAAATGGAGCGGGAAAATGGAAGAGAGTTGAAGTGAATCCTGAAGAACACATTCACATGCCCTCTTTCGCAGATGAATTATCATCAATTGAATCAAGTGTTGCTTGCTTTGACGAATATCTCAAAAAATTGTTACTAAACAGTTTTCAGAGGGCAAACCGTGGTGGGAAATTCATGTGTTCCAGTATCCAACCAGCAGTGCAGTTTGCAGTTTAATTTTCAAGATTCATCATTCCATAGCTGATGGCTTCTCTCTCATGAGTGCCATTCTATCTTTCCTTAAGAGAGCCGATGACCCTTCACTTCCAATAACATTTCCATTCACAAATCGTTCCGAACAGCCTAAAAAGATTAGCGTTTTTAGCCATTTGCCTCATTTCTTCTCCTCAATTACCAAACTGTATCAGGTTTTGGATGGAACATAATGAAAAGCACAGTAATTAGGGATGATCGAACCCCGATAAGGCCAAGAGGAGATTGCATGGAACTCACAAAGCCACTTGCAATATCCATTATGACATTTTCCCTTAAACAAATCAAAGAAATTAAGACAAAGCTAAAAGTAGTACATATAATATATGCCTTTTTGTGCCAAATTTACATTCCTCGTTTTATCAATCTTTGTTTTGTTCAAGTCCCGTAGTGATGTAATATGATTTGAGATCATAAAAGAGGTCTATGTTCGAACTCTTGCAACATATTTCCTACACATTTGATATTAATAATTCACGCTTTAAATTTTTACTTTATGATTGGCAGACTGTTAATGACGTGCTCAGTGGAATTATCTTTTTGGGAATTCGATTGTACATGCGAGAGATTAGCTATGAATCAGGAAAAGAAAATTCAACTGCATTGATTCTACAGAATACTAGGAAGAAAAAGGCCTATAAGTCAGTGCAGGACATGGAGAAAACTGATGTAGATACGCCATGGGGGAATAGAATCGCCTTCTTGCCGGTGTCTATTCCCAAACTAAATGATATTCACTTTCAAATCCTCTTGAGTTTGTCAAGGAAGCACAAAAGTCGATTATGA

mRNA sequence

ATGGAGATTTTCGAAGATTTAGTCCCAGTGAGTCCTTTGAGCCAATACTTCAACACCTCAGTGATGTGTGTTTCTGTTCTGGGCGTCATCGAACTTGAAACTTCAGTCTCTCCCTCCGATGCCTCATTAGTGATCACTGATAGTCTCCTCCCTGCCAACCCACGCTTCAGTTCAATCATGGTTCTCTCTCTCTCTCTCTCATTAGTACAGGGCAAACCGTGGTGGGAAATTCATGTGTTCCAGTATCCAACCAGCAGTGCAGTTTGCAGTTTAATTTTCAAGATTCATCATTCCATAGCTGATGGCTTCTCTCTCATGAGTGCCATTCTATCTTTCCTTAAGAGAGCCGATGACCCTTCACTTCCAATAACATTTCCATTCACAAATCGTTCCGAACAGCCTAAAAAGATTAGCGTTTTTAGCCATTTGCCTCATTTCTTCTCCTCAATTACCAAACTGTATCAGACTGTTAATGACGTGCTCAGTGGAATTATCTTTTTGGGAATTCGATTGTACATGCGAGAGATTAGCTATGAATCAGGAAAAGAAAATTCAACTGCATTGATTCTACAGAATACTAGGAAGAAAAAGGCCTATAAGTCAGTGCAGGACATGGAGAAAACTGATGTAGATACGCCATGGGGGAATAGAATCGCCTTCTTGCCGGTGTCTATTCCCAAACTAAATGATATTCACTTTCAAATCCTCTTGAGTTTGTCAAGGAAGCACAAAAGTCGATTATGA

Coding sequence (CDS)

ATGGAGATTTTCGAAGATTTAGTCCCAGTGAGTCCTTTGAGCCAATACTTCAACACCTCAGTGATGTGTGTTTCTGTTCTGGGCGTCATCGAACTTGAAACTTCAGTCTCTCCCTCCGATGCCTCATTAGTGATCACTGATAGTCTCCTCCCTGCCAACCCACGCTTCAGTTCAATCATGGTTCTCTCTCTCTCTCTCTCATTAGTACAGGGCAAACCGTGGTGGGAAATTCATGTGTTCCAGTATCCAACCAGCAGTGCAGTTTGCAGTTTAATTTTCAAGATTCATCATTCCATAGCTGATGGCTTCTCTCTCATGAGTGCCATTCTATCTTTCCTTAAGAGAGCCGATGACCCTTCACTTCCAATAACATTTCCATTCACAAATCGTTCCGAACAGCCTAAAAAGATTAGCGTTTTTAGCCATTTGCCTCATTTCTTCTCCTCAATTACCAAACTGTATCAGACTGTTAATGACGTGCTCAGTGGAATTATCTTTTTGGGAATTCGATTGTACATGCGAGAGATTAGCTATGAATCAGGAAAAGAAAATTCAACTGCATTGATTCTACAGAATACTAGGAAGAAAAAGGCCTATAAGTCAGTGCAGGACATGGAGAAAACTGATGTAGATACGCCATGGGGGAATAGAATCGCCTTCTTGCCGGTGTCTATTCCCAAACTAAATGATATTCACTTTCAAATCCTCTTGAGTTTGTCAAGGAAGCACAAAAGTCGATTATGA

Protein sequence

MEIFEDLVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSIMVLSLSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPKKISVFSHLPHFFSSITKLYQTVNDVLSGIIFLGIRLYMREISYESGKENSTALILQNTRKKKAYKSVQDMEKTDVDTPWGNRIAFLPVSIPKLNDIHFQILLSLSRKHKSRL

Homology

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BLAST of Sgr025151 vs. NCBI nr
Match: XP_022147882.1 (O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransferase WSD1-like [Momordica charantia])

HSP 1 Score: 250.0 bits (637), Expect = 2.2e-62
Identity = 151/328 (46.04%), Postives = 181/328 (55.18%), Query Frame = 0


Query: 1   MEIFED-LVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSI 60
           MEI ED   P+SPLSQYFNTS MCVSVLGV+ELE+ +SP DA  ++TD LLPANPRF+SI
Sbjct: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60

Query: 61  MVLSLSLS--------------------------------------------LVQGKPWW 120
           MV   S                                                Q  P W
Sbjct: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120

Query: 121 EIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPK 180
           EIHVF YPTS A CS+IFK+HHSIADGF LM+ +LS LKRADDPSLP+TFP    S+QPK
Sbjct: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180

Query: 181 ---KISVFSHLPHFFSSI------------------------------------------ 231
              +  + SHLP   SS+                                          
Sbjct: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240

[Close]

BLAST of Sgr025151 vs. NCBI nr
Match: XP_022952997.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 244.2 bits (622), Expect = 1.2e-60
Identity = 157/331 (47.43%), Postives = 178/331 (53.78%), Query Frame = 0


Query: 1   MEIFED-LVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSI 60
           MEI ED   P+SPLSQYFNTS MCVSVLG IELE  +S S A  + T  L+PANPRFSSI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFSSI 60

Query: 61  MV-------------------------LSLSLS-------------------LVQGKPWW 120
           MV                           + LS                     Q  P W
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIPLW 120

Query: 121 EIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPK 180
           EIHVF YPT+ A C+LIFK+HHSIADGF LM+ +LSFLKRADDPSLP+TFP   RS+QP+
Sbjct: 121 EIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQPE 180

Query: 181 KISVFSHLPHF----FSSI----------------------------------------- 231
               F  L HF    F S+                                         
Sbjct: 181 NAHDFGRLSHFPACLFLSLRNFLSNFGYSIANTFVEDDPTPIKHEGDSMQLVKPIAISTM 240

[Close]

BLAST of Sgr025151 vs. NCBI nr
Match: XP_011659709.2 (O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_017048 [Cucumis sativus])

HSP 1 Score: 240.4 bits (612), Expect = 1.7e-59
Identity = 156/332 (46.99%), Postives = 181/332 (54.52%), Query Frame = 0


Query: 1   MEIFED-LVPVSPLSQYFNTSVMCVSVLGVIELETSVSP-SDASLVITDSLLPANPRFSS 60
           MEI+ED   P+SPLSQYFNT  MCVSVLGVIELET +S   D S ++TD L+PANPRF+S
Sbjct: 1   MEIYEDSTAPMSPLSQYFNTCEMCVSVLGVIELETPISSWEDISSIVTDVLVPANPRFTS 60

Query: 61  IMV------------------------LSLSLS-------------------LVQGKPWW 120
           IMV                          + LS                     Q  P W
Sbjct: 61  IMVKEKTEERKWKTTKVNPKDHIYIPNFPMELSSYEYDAYFDEYATKTATKPFSQAIPLW 120

Query: 121 EIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQP- 180
           EIHVF YPT+ A CS+IFK+HHSIADGF LM+ +LS LKRADDPSLP+TFP   RS+QP 
Sbjct: 121 EIHVFNYPTTHATCSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQRSKQPG 180

Query: 181 --KKISVFSHLP-HFFSSIT---------------------------------------- 231
              K    SH P  FFSSI+                                        
Sbjct: 181 NEPKFFRLSHFPARFFSSISNFVLNFGWSIMKNTFVEDDPTPIKPQKDSMQLVKPIAIST 240

[Close]

BLAST of Sgr025151 vs. NCBI nr
Match: XP_023511661.1 (O-acyltransferase WSD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 239.2 bits (609), Expect = 3.8e-59
Identity = 155/331 (46.83%), Postives = 178/331 (53.78%), Query Frame = 0


Query: 1   MEIFED-LVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSI 60
           MEI ED   P+SPLSQYFNTS MCVSVLG IELE  +S S A  + T  L+PANPRFSSI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAVSIGTHVLIPANPRFSSI 60

Query: 61  MV-------------------------LSLSLS-------------------LVQGKPWW 120
           MV                           + LS                     Q  P W
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFNQSIPLW 120

Query: 121 EIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPK 180
           EIHVF YPTS A C+LIFK+HHSIADGF LM+ +LSFLKRADDPSLP+TFP   RS+Q +
Sbjct: 121 EIHVFNYPTSHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQLE 180

Query: 181 KISVFSHLPHF----FSSI----------------------------------------- 231
               F  L HF    F S+                                         
Sbjct: 181 YAHDFGRLSHFPARLFLSLCNFISNFGYSIANTFTEDDPTPIKREGDSMQLVKPIAISTM 240

[Close]

BLAST of Sgr025151 vs. NCBI nr
Match: XP_038887587.1 (O-acyltransferase WSD1-like isoform X2 [Benincasa hispida])

HSP 1 Score: 230.7 bits (587), Expect = 1.4e-56
Identity = 146/328 (44.51%), Postives = 176/328 (53.66%), Query Frame = 0


Query: 5   EDLVPVSPLSQYFNTSVMCVSVLGVIELETSVSP-SDASLVITDSLLPANPRFSSIMVLS 64
           E   P+SP+SQYFNTS MCVSVLGVIELET +S   + S +  D L+P+NPRF+S+MV  
Sbjct: 6   ESTAPMSPMSQYFNTSEMCVSVLGVIELETPISAWEEISSIAIDVLIPSNPRFTSVMVKE 65

Query: 65  LS--------------------------------------------LSLVQGKPWWEIHV 124
            S                                                Q  P WEIHV
Sbjct: 66  SSNGERKWKTIKINPKDHIYIPNFPPELSPYEYDAYFDEYATQTATKPFSQSIPLWEIHV 125

Query: 125 FQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPKKISV 184
           F YPTS A CS+IFK+HHSIADGF LM+ +LS LKRADDPSLP+TFP   RS+QP+    
Sbjct: 126 FNYPTSHATCSIIFKVHHSIADGFCLMNILLSCLKRADDPSLPLTFPSRQRSKQPENELN 185

Query: 185 F---SHLP-HFFSSIT-------------------------------------------- 231
           F   S+ P H FSS++                                            
Sbjct: 186 FRRRSYFPGHLFSSVSNFLLNFGWSIMKNTIVEDDPTPIKPERDSMQLVKPIAISTITFP 245

[Close]

BLAST of Sgr025151 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 53.9 bits (128), Expect = 3.0e-06
Identity = 34/113 (30.09%), Postives = 56/113 (49.56%), Query Frame = 0


Query: 56  FSSIMVLSLSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKR 115
           ++S M LS    +   KP WE H+ +  TS A    + + HHS+ DG SLMS +L+  ++
Sbjct: 106 YTSNMALS---PMDMSKPLWEFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRK 165

Query: 116 ADDPSLPITFP-FTNRSEQPKKISVFSHLPHFFSSITKLYQTVNDVLSGIIFL 168
             DP     FP F    +   K   FS +   +  +  ++ T  +V+  I+F+
Sbjct: 166 TCDPE---AFPTFVAPKKNKAKNVCFSLVAWLWFIVRLMFHTCVEVIKSIVFI 212

[Close]

BLAST of Sgr025151 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 3.9e-06
Identity = 23/68 (33.82%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 64  LSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPI 123
           + + L   +P WE+H+    TS A    + KIHHS+ DG S+MS +L+ +++  +P    
Sbjct: 116 MKIPLDTSRPLWELHLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELP 175

Query: 124 TFPFTNRS 132
           + P+  RS
Sbjct: 176 SLPYQYRS 183

[Close]

BLAST of Sgr025151 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 8.6e-06
Identity = 49/227 (21.59%), Postives = 90/227 (39.65%), Query Frame = 0


Query: 2   EIFEDLVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSIMV 61
           E+ E+  P+SP ++ F+       ++ V+ L+  + P D  +      L  +PRFSS +V
Sbjct: 20  EVEEEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEP-DMIIEGIKQTLMRHPRFSSKLV 79

Query: 62  LS-----------------------------------------------LSLSLVQGKPW 121
            +                                                ++ L   KP 
Sbjct: 80  NNCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPL 139

Query: 122 WEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNR-SEQ 176
           WE+H+    TS A    + +IHHS+ DG S+MS +L+  ++  +P+   + P+ NR S  
Sbjct: 140 WEVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSG 199

[Close]

BLAST of Sgr025151 vs. ExPASy Swiss-Prot
Match: Q93ZR6 (Wax ester synthase/diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=WSD1 PE=1 SV=1)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 24/55 (43.64%), Postives = 32/55 (58.18%), Query Frame = 0


Query: 65  SLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDP 120
           ++ +   KP WE H+    TS A    I KIHHSI DG SLMS +L+  ++  DP
Sbjct: 115 NIPMDMSKPLWEFHLLNMKTSKAESLAIVKIHHSIGDGMSLMSLLLACSRKISDP 169

[Close]

BLAST of Sgr025151 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 1.5e-05
Identity = 26/63 (41.27%), Postives = 37/63 (58.73%), Query Frame = 0


Query: 57  SSIMVLSLSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRA 116
           S +  + + LS    KP WE+H+    TS+A    I KIHHS+ DG SLMS +L+  ++ 
Sbjct: 108 SKLTTIPMDLS----KPLWEMHLLGVKTSNAESYAILKIHHSLGDGMSLMSLLLACTRKT 166

Query: 117 DDP 120
            DP
Sbjct: 168 SDP 166

[Close]

BLAST of Sgr025151 vs. ExPASy TrEMBL
Match: A0A6J1D2I7 (Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.0e-62
Identity = 151/328 (46.04%), Postives = 181/328 (55.18%), Query Frame = 0


Query: 1   MEIFED-LVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSI 60
           MEI ED   P+SPLSQYFNTS MCVSVLGV+ELE+ +SP DA  ++TD LLPANPRF+SI
Sbjct: 1   MEILEDSTTPMSPLSQYFNTSEMCVSVLGVLELESPISPPDAISIVTDVLLPANPRFTSI 60

Query: 61  MVLSLSLS--------------------------------------------LVQGKPWW 120
           MV   S                                                Q  P W
Sbjct: 61  MVKEKSTGERKWKTAEVNPRDHIYVPTFPNDLSPYEYDAYFDEYATKTATKPFSQSIPLW 120

Query: 121 EIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPK 180
           EIHVF YPTS A CS+IFK+HHSIADGF LM+ +LS LKRADDPSLP+TFP    S+QPK
Sbjct: 121 EIHVFNYPTSHAACSIIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRKHSKQPK 180

Query: 181 ---KISVFSHLPHFFSSI------------------------------------------ 231
              +  + SHLP   SS+                                          
Sbjct: 181 NDARFGLLSHLPRRISSVVSNFGWSVMKNTFVEDDPTPIKPEGDCMQLVKPIAISTMTFS 240

[Close]

BLAST of Sgr025151 vs. ExPASy TrEMBL
Match: A0A6J1GLZ3 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 PE=4 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 5.7e-61
Identity = 157/331 (47.43%), Postives = 178/331 (53.78%), Query Frame = 0


Query: 1   MEIFED-LVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSI 60
           MEI ED   P+SPLSQYFNTS MCVSVLG IELE  +S S A  + T  L+PANPRFSSI
Sbjct: 1   MEILEDSAAPMSPLSQYFNTSAMCVSVLGFIELEAPISASHAMNIGTHVLIPANPRFSSI 60

Query: 61  MV-------------------------LSLSLS-------------------LVQGKPWW 120
           MV                           + LS                     Q  P W
Sbjct: 61  MVKEKWFGERKWKMVEVNLEDHIYTPKFPVDLSPYEYDAYFDEYATNTATKPFSQSIPLW 120

Query: 121 EIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPK 180
           EIHVF YPT+ A C+LIFK+HHSIADGF LM+ +LSFLKRADDPSLP+TFP   RS+QP+
Sbjct: 121 EIHVFNYPTTHAPCTLIFKVHHSIADGFCLMNTLLSFLKRADDPSLPLTFPSRKRSKQPE 180

Query: 181 KISVFSHLPHF----FSSI----------------------------------------- 231
               F  L HF    F S+                                         
Sbjct: 181 NAHDFGRLSHFPACLFLSLRNFLSNFGYSIANTFVEDDPTPIKHEGDSMQLVKPIAISTM 240

[Close]

BLAST of Sgr025151 vs. ExPASy TrEMBL
Match: A0A2N9FDD5 (Diacylglycerol O-acyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13020 PE=4 SV=1)

HSP 1 Score: 199.5 bits (506), Expect = 1.6e-47
Identity = 119/294 (40.48%), Postives = 164/294 (55.78%), Query Frame = 0


Query: 5   EDLVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSIMV--- 64
           E L P SP +QYFN+S + VS++ V+E E  ++  +A  ++ D  LP NPRFSSIMV   
Sbjct: 6   EGLAPASPSAQYFNSSALSVSIIAVLESEVPINDENAMSLLKDVFLPINPRFSSIMVENE 65

Query: 65  --------LSLSLS--------------------------------LVQGKPWWEIHVFQ 124
                   + + L                                   Q KP WE+H+ +
Sbjct: 66  KGEKQWKKVEIKLEDHIDIPIFPTGLSPTSYDKYLDDYISNMAMDRFPQHKPLWEVHIVK 125

Query: 125 YPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPK------ 184
           YPTS+A  ++IFK+HH++ DG+SLM ++LS L+RAD+PSLP+TFP +++S +PK      
Sbjct: 126 YPTSNAAGNVIFKLHHALGDGYSLMGSLLSCLQRADNPSLPLTFP-SHQSSKPKNGREKD 185

Query: 185 -------------------KISVFSHLPHFFSSITKLYQTVNDVLSGIIFLGIRLYMREI 231
                                 +FS L       TKL  T+NDV++GIIFLG RLYM+EI
Sbjct: 186 DRTPIRSGNEGLEYRPIVISTMIFS-LDRIKHIKTKLGATINDVITGIIFLGTRLYMQEI 245

[Close]

BLAST of Sgr025151 vs. ExPASy TrEMBL
Match: V4TJ95 (Diacylglycerol O-acyltransferase OS=Citrus clementina OX=85681 GN=CICLE_v10015126mg PE=4 SV=1)

HSP 1 Score: 192.2 bits (487), Expect = 2.6e-45
Identity = 123/325 (37.85%), Postives = 162/325 (49.85%), Query Frame = 0


Query: 5   EDLVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSIMVL-- 64
           E++ PVSP  QY N+S + +SVLGV+E E  +  S    ++ D  LP NPRFSSIMV+  
Sbjct: 7   EEMEPVSPSGQYLNSSALSLSVLGVLESEIPIDDSQCFSLLKDIFLPINPRFSSIMVVDE 66

Query: 65  ----------------------------------------SLSLSLV-QGKPWWEIHVFQ 124
                                                    + + L  Q +P WE+H+ +
Sbjct: 67  NGEKQWKRVEVKLRNHVKVPIFPSGLSPEAYDKYFDDYISEIGMELFPQSQPLWEVHIIK 126

Query: 125 YPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNRSEQPKK----I 184
           YPTS A   LIFK+HHS+ DGFSLM A+LS L+RADDPS+P+TFP  NR    KK     
Sbjct: 127 YPTSHAAGFLIFKLHHSLGDGFSLMGALLSCLQRADDPSVPLTFPSVNRFPSNKKDGNNS 186

Query: 185 SVFSHLPHFFSSI----------------------------------------------- 231
           ++FS++   F  +                                               
Sbjct: 187 NIFSNMYKTFCVVSETVSDFCWSFVKSAWLQDDRTPIYSGDDGIEFRPVSVATTAFSLDQ 246

[Close]

BLAST of Sgr025151 vs. ExPASy TrEMBL
Match: A0A5A7SPR9 (Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold139G002950 PE=4 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 5.7e-45
Identity = 109/218 (50.00%), Postives = 128/218 (58.72%), Query Frame = 0


Query: 70  QGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTN 129
           Q  P WEIHVF YPT+ A CS+IFK+HHSIADGF LM+ +LS LKRADDPSLP+TFP   
Sbjct: 55  QAIPLWEIHVFNYPTTHATCSVIFKVHHSIADGFCLMNTLLSCLKRADDPSLPLTFPSRQ 114

Query: 130 RSEQPKKISVFSHLPH----FFSSIT---------------------------------- 189
           RS+QPK    F  L H    FFSS++                                  
Sbjct: 115 RSKQPKNKLDFCRLSHFPARFFSSVSNFVLNFGWSIMKNTFVEDDPTPIKPQEDSMQLVK 174

Query: 190 -------------------KLYQTVNDVLSGIIFLGIRLYMREISYESGKENSTALILQN 231
                              KL  +VNDVL+GIIFLG+RLYM+E + ES + NS+ALIL N
Sbjct: 175 PIAISTMTFSLDQIKQIKNKLNASVNDVLTGIIFLGVRLYMQEHNPESSEANSSALILLN 234

[Close]

BLAST of Sgr025151 vs. TAIR 10
Match: AT1G72110.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.1e-07
Identity = 34/113 (30.09%), Postives = 56/113 (49.56%), Query Frame = 0


Query: 56  FSSIMVLSLSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKR 115
           ++S M LS    +   KP WE H+ +  TS A    + + HHS+ DG SLMS +L+  ++
Sbjct: 106 YTSNMALS---PMDMSKPLWEFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRK 165

Query: 116 ADDPSLPITFP-FTNRSEQPKKISVFSHLPHFFSSITKLYQTVNDVLSGIIFL 168
             DP     FP F    +   K   FS +   +  +  ++ T  +V+  I+F+
Sbjct: 166 TCDPE---AFPTFVAPKKNKAKNVCFSLVAWLWFIVRLMFHTCVEVIKSIVFI 212

[Close]

BLAST of Sgr025151 vs. TAIR 10
Match: AT1G72110.2 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 53.9 bits (128), Expect = 2.1e-07
Identity = 34/113 (30.09%), Postives = 56/113 (49.56%), Query Frame = 0


Query: 56  FSSIMVLSLSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKR 115
           ++S M LS    +   KP WE H+ +  TS A    + + HHS+ DG SLMS +L+  ++
Sbjct: 106 YTSNMALS---PMDMSKPLWEFHLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRK 165

Query: 116 ADDPSLPITFP-FTNRSEQPKKISVFSHLPHFFSSITKLYQTVNDVLSGIIFL 168
             DP     FP F    +   K   FS +   +  +  ++ T  +V+  I+F+
Sbjct: 166 TCDPE---AFPTFVAPKKNKAKNVCFSLVAWLWFIVRLMFHTCVEVIKSIVFI 212

[Close]

BLAST of Sgr025151 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.8e-07
Identity = 23/68 (33.82%), Postives = 39/68 (57.35%), Query Frame = 0


Query: 64  LSLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPI 123
           + + L   +P WE+H+    TS A    + KIHHS+ DG S+MS +L+ +++  +P    
Sbjct: 116 MKIPLDTSRPLWELHLLDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELP 175

Query: 124 TFPFTNRS 132
           + P+  RS
Sbjct: 176 SLPYQYRS 183

[Close]

BLAST of Sgr025151 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 52.4 bits (124), Expect = 6.1e-07
Identity = 49/227 (21.59%), Postives = 90/227 (39.65%), Query Frame = 0


Query: 2   EIFEDLVPVSPLSQYFNTSVMCVSVLGVIELETSVSPSDASLVITDSLLPANPRFSSIMV 61
           E+ E+  P+SP ++ F+       ++ V+ L+  + P D  +      L  +PRFSS +V
Sbjct: 20  EVEEEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEP-DMIIEGIKQTLMRHPRFSSKLV 79

Query: 62  LS-----------------------------------------------LSLSLVQGKPW 121
            +                                                ++ L   KP 
Sbjct: 80  NNCNNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANADVFLESYVSDLTTIPLDTSKPL 139

Query: 122 WEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDPSLPITFPFTNR-SEQ 176
           WE+H+    TS A    + +IHHS+ DG S+MS +L+  ++  +P+   + P+ NR S  
Sbjct: 140 WEVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSG 199

[Close]

BLAST of Sgr025151 vs. TAIR 10
Match: AT5G37300.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 52.0 bits (123), Expect = 8.0e-07
Identity = 24/55 (43.64%), Postives = 32/55 (58.18%), Query Frame = 0


Query: 65  SLSLVQGKPWWEIHVFQYPTSSAVCSLIFKIHHSIADGFSLMSAILSFLKRADDP 120
           ++ +   KP WE H+    TS A    I KIHHSI DG SLMS +L+  ++  DP
Sbjct: 115 NIPMDMSKPLWEFHLLNMKTSKAESLAIVKIHHSIGDGMSLMSLLLACSRKISDP 169

The following BLAST results are available for this feature:

BLAST of Sgr025151 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022147882.1	2.2e-62	46.04	O-acyltransferase WSD1-like [Momordica charantia] >XP_022147883.1 O-acyltransfer...	[more]
XP_022952997.1	1.2e-60	47.43	O-acyltransferase WSD1-like [Cucurbita moschata]	[more]
XP_011659709.2	1.7e-59	46.99	O-acyltransferase WSD1 [Cucumis sativus] >KGN43701.2 hypothetical protein Csa_01...	[more]
XP_023511661.1	3.8e-59	46.83	O-acyltransferase WSD1-like [Cucurbita pepo subsp. pepo]	[more]
XP_038887587.1	1.4e-56	44.51	O-acyltransferase WSD1-like isoform X2 [Benincasa hispida]	[more]

BLAST of Sgr025151 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C7H4	3.0e-06	30.09	Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B3	3.9e-06	33.82	Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3...	[more]
Q9M3B2	8.6e-06	21.59	Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3...	[more]
Q93ZR6	1.1e-05	43.64	Wax ester synthase/diacylglycerol acyltransferase 1 OS=Arabidopsis thaliana OX=3...	[more]
Q5KS41	1.5e-05	41.27	Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Sgr025151 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D2I7	1.0e-62	46.04	Diacylglycerol O-acyltransferase OS=Momordica charantia OX=3673 GN=LOC111016711 ...	[more]
A0A6J1GLZ3	5.7e-61	47.43	Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455512 P...	[more]
A0A2N9FDD5	1.6e-47	40.48	Diacylglycerol O-acyltransferase OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS13020 P...	[more]
V4TJ95	2.6e-45	37.85	Diacylglycerol O-acyltransferase OS=Citrus clementina OX=85681 GN=CICLE_v1001512...	[more]
A0A5A7SPR9	5.7e-45	50.00	Diacylglycerol O-acyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27...	[more]

BLAST of Sgr025151 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G72110.1	2.1e-07	30.09	O-acyltransferase (WSD1-like) family protein	[more]
AT1G72110.2	2.1e-07	30.09	O-acyltransferase (WSD1-like) family protein	[more]
AT3G49190.1	2.8e-07	33.82	O-acyltransferase (WSD1-like) family protein	[more]
AT3G49200.1	6.1e-07	21.59	O-acyltransferase (WSD1-like) family protein	[more]
AT5G37300.1	8.0e-07	43.64	O-acyltransferase (WSD1-like) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR31650:SF34	O-ACYLTRANSFERASE WSD1-LIKE	coord: 68..152
None	No IPR available	PANTHER	PTHR31650:SF34	O-ACYLTRANSFERASE WSD1-LIKE	coord: 6..61
None	No IPR available	PANTHER	PTHR31650:SF34	O-ACYLTRANSFERASE WSD1-LIKE	coord: 151..231
IPR045034	O-acyltransferase WSD1-like	PANTHER	PTHR31650	O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN	coord: 68..152
IPR045034	O-acyltransferase WSD1-like	PANTHER	PTHR31650	O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN	coord: 151..231 coord: 6..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr025151.1	Sgr025151.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045017	glycerolipid biosynthetic process
molecular_function	GO:0008374	O-acyltransferase activity