Sgr025130 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr025130 vs. NCBI nr
Match: XP_022147829.1 (uncharacterized protein LOC111016673 [Momordica charantia])

HSP 1 Score: 710.3 bits (1832), Expect = 1.6e-200
Identity = 390/591 (65.99%), Postives = 434/591 (73.43%), Query Frame = 0

Query: 78  LIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAYDLIE 137
           +I+RAL RLKMLKKKR+SI+RQLREDL ELI+NG+QQIAF+RVEQLI DESL+EAYDLIE
Sbjct: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60

Query: 138 NFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQSFAM 197
           NFCEFIL+NFSH+RKHKTCPDD+IEAISSLIFASARCGD PELK VRKLFEER+G+SFA 
Sbjct: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120

Query: 198 AAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDWHQKQVL 257
            AVELCPGNLVNPQIKEKLL++PVSD+EKQ L+SEIARDCF PAILALEYCPDWHQKQVL
Sbjct: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180

Query: 258 ENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYLDCQSSP 317
           ENGDQTHCE KEE  +QDSNAE+ ER+V+C   S                          
Sbjct: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPS-------------------------- 240

Query: 318 TLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDASSPEFP 377
                      +EV S+S                       ATLE       DASSPE  
Sbjct: 241 -----------QEVCSSS-----------------------ATLE-------DASSPECS 300

Query: 378 PFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMRETVQDGNDQSLI-EHHDN 437
           PF EET+VYLDDVVELPNP  E+G LWDQR FKFKSPVT MRE VQ GN Q LI EHH+N
Sbjct: 301 PFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNN 360

Query: 438 SKKKLVSERSCQSINGSPKRLRRRSTCRENNSLSHTKKKLMKCCCLSSHSCCLSSEMENY 497
           SKK+ VSERS QS+N SPKR  RRST +E++SLSH KK+LMKCCCLS HS  L S MENY
Sbjct: 361 SKKRSVSERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENY 420

Query: 498 CLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLSGKDGHSHIDHISWTG 557
           CLEQP YVY       SEV+ISEKKKV  +FI       LDN+LSGKD H          
Sbjct: 421 CLEQPSYVY-------SEVLISEKKKVDPHFI-------LDNSLSGKDRH---------- 480

Query: 558 DSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKETNAKPKEFSTKGKHEPSL 617
                +EFD  PRT KIR+CE+GT+VYD+FVYSHCQP ENKETNAKP+E STK KHE SL
Sbjct: 481 ----RVEFDTLPRTEKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSL 496

Query: 618 GFNVMKNNF---VKAADKYPSHVHPKLPDYDEITAKFIALKQEYLQRKQQQ 665
           GFN MK++F    KAADKYPSHVHPKLPDYDEI AKFIALK+EY QRKQ Q
Sbjct: 541 GFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAAKFIALKREYSQRKQHQ 496

BLAST of Sgr025130 vs. NCBI nr
Match: XP_038888606.1 (uncharacterized protein LOC120078409 [Benincasa hispida])

HSP 1 Score: 703.7 bits (1815), Expect = 1.5e-198
Identity = 392/613 (63.95%), Postives = 447/613 (72.92%), Query Frame = 0

Query: 62  MFGILFGWRKASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVE 121
           MFG LFGWRKASKCKKLIR ALCRL+MLKKKR+SIV+QLREDL EL+NNG+QQIAF RVE
Sbjct: 1   MFGFLFGWRKASKCKKLIRTALCRLQMLKKKRYSIVKQLREDLFELVNNGYQQIAFRRVE 60

Query: 122 QLIGDESLIEAYDLIENFCEFILVNFSHIRKHK------TCPDDIIEAISSLIFASARCG 181
           QLI DE L+EAYDLIENFCEFILV FSHI+KHK      TCPDDIIEAISSLIFASARCG
Sbjct: 61  QLIQDEILMEAYDLIENFCEFILVKFSHIKKHKYFMVFRTCPDDIIEAISSLIFASARCG 120

Query: 182 DFPELKLVRKLFEERYGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIAR 241
           DFPELK VRKLFE+R+G+SFA+AAVELCPGNLVN QIKEKLL++PVSD+EKQR +++IAR
Sbjct: 121 DFPELKSVRKLFEKRFGRSFAVAAVELCPGNLVNSQIKEKLLLKPVSDHEKQRFINDIAR 180

Query: 242 DCFHPAILALEYCPDWHQKQVLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNG 301
           DCF+P+ILALEY PDWHQKQV +N D+T+ E KEE        E LE+ VI  +SS  N 
Sbjct: 181 DCFYPSILALEYSPDWHQKQVHDNEDETNGEGKEE-------PEKLEKVVISLNSSRDNN 240

Query: 302 SDIVDPQCHSTSSSYLDCQSSPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGW 361
            D+VD +CHSTSSS L CQSS        PN RE+V                        
Sbjct: 241 RDVVDARCHSTSSSSLVCQSS--------PNEREDV------------------------ 300

Query: 362 IAAATLEDGLDRDNDASSPEFPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPV 421
                   GLDR+NDASSPE  PF +E+IVYLD++VEL +   E GD  DQRFFKFKS V
Sbjct: 301 --------GLDRENDASSPEILPFCDESIVYLDNIVELSDLSMEYGDSLDQRFFKFKSLV 360

Query: 422 TSMRETVQDGNDQSLIEHHDNSKKKLVSERSCQSINGSPKRLRRRSTCR--ENNSLSHTK 481
           TSM E V+DGNDQSLIE HD+S  KLVS RS Q I GSPK LR  S CR  EN+SL++TK
Sbjct: 361 TSMGENVRDGNDQSLIEKHDDSNTKLVSGRSNQIIIGSPKELRIVSMCREQENHSLNNTK 420

Query: 482 KKLMKCCCLSSHSCCLSSEMENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAH 541
           KK MKCCCLS HS  LSSE++NYCLEQPCYVYS+C  DD             Y +RS A 
Sbjct: 421 KKFMKCCCLSCHS--LSSELQNYCLEQPCYVYSECRTDD-------------YVVRSSAV 480

Query: 542 NRLDNNLSGKDGHSHIDHISWTGDSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQP 601
              D +LS KD        SW  DS+EEIEFD F RT K  +   GTVVYD+FVYSHCQP
Sbjct: 481 KHTDYDLSSKD--------SWNEDSNEEIEFDTFSRTKKASNYGNGTVVYDVFVYSHCQP 540

Query: 602 YENKETNAKPKEFSTKGKHEPSLGFNVMKNNF---VKAADKYPSHVHPKLPDYDEITAKF 661
            ENKETNAKP+E  T GK+E S+GFN ++N F   +K ADKYPSHVHPKLPDYDEI AKF
Sbjct: 541 VENKETNAKPEELRTNGKYETSVGFNGIQNKFTKCMKGADKYPSHVHPKLPDYDEIAAKF 543

Query: 662 IALKQEYLQRKQQ 664
           IALK+EYLQ+  +
Sbjct: 601 IALKREYLQKSMK 543

BLAST of Sgr025130 vs. NCBI nr
Match: XP_016901704.1 (PREDICTED: uncharacterized protein LOC103495691 [Cucumis melo])

HSP 1 Score: 614.0 bits (1582), Expect = 1.5e-171
Identity = 359/606 (59.24%), Postives = 408/606 (67.33%), Query Frame = 0

Query: 62  MFGILFGWRKASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVE 121
           MFG LFGWRKASKCKKLI+ ALCRLK+LKKKR+SIV+ LREDL ELINNG+QQIAF RVE
Sbjct: 1   MFGFLFGWRKASKCKKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVE 60

Query: 122 QLIGDESLIEAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 181
           QL+ DE+L+E YDLIEN CEFILV FSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK
Sbjct: 61  QLLQDETLMEVYDLIENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELK 120

Query: 182 LVRKLFEERYGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPA 241
            VR LFEER+G+SFA+AAVEL PGNLVN QIKEKL+MQ VS++EKQRL++EIARDCF PA
Sbjct: 121 CVRMLFEERFGKSFAVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPA 180

Query: 242 ILALEYCPDWHQKQVLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDP 301
           +LALEY PD  QKQVL+N DQT  E K E       +E+LER VI  DS+  +   ++DP
Sbjct: 181 VLALEYRPDSDQKQVLQNEDQTTVEGKVE-------SEELERVVISLDSARDSNCGVLDP 240

Query: 302 QCHSTSSSYLDCQSSPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATL 361
            CHSTSSS   CQS P                                            
Sbjct: 241 WCHSTSSSSSVCQSFP-------------------------------------------- 300

Query: 362 EDGLDRDNDASSPEFPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMR-E 421
                  NDASS EF PF EE IVY DDVVEL +P TE GDL DQRFFKFK  +TS R E
Sbjct: 301 -------NDASSSEFLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREE 360

Query: 422 TVQDGNDQSLIEHHDNSKKKLVSERSCQSINGSPKRLRRRSTCR--ENNSLSHTKKKLMK 481
            V+D +DQSLIE HD S KK VS RS Q ING PK   R+  CR  EN+SL+HTKKK  K
Sbjct: 361 NVRDRDDQSLIEKHDASNKKSVSGRSNQIINGPPKEPSRKFMCREQENHSLNHTKKKFTK 420

Query: 482 CCCLSSHSCCLSSEMENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDN 541
           CCCLS HS  LS E++NY LEQ CYVYS+C  D              Y IRS   NRLD 
Sbjct: 421 CCCLSCHS--LSFELQNYSLEQQCYVYSECRTD-------------YYIIRSSEANRLDY 480

Query: 542 NLSGKDGHSHIDHISWTGDSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKE 601
           +LS K       H+ W  +S+EEIEF  F R  + R+  IGTVVYD+FVYSHCQP ENKE
Sbjct: 481 DLSSK-------HL-WNAESNEEIEFITFSRAKEQRNYGIGTVVYDVFVYSHCQPDENKE 514

Query: 602 TNAKPKEFSTKGKHEPSLGFNVMKNNFVKAADKYPSHVHPKLPDYDEITAKFIALKQEYL 661
           TN K +E      HEPS     M     K A KYPSHVHPKLPDYDEI  +FI+LK+EYL
Sbjct: 541 TNTKLEE------HEPSKFTKCM-----KGAHKYPSHVHPKLPDYDEIAVEFISLKREYL 514

Query: 662 QRKQQQ 665
           QR  ++
Sbjct: 601 QRSMKR 514

BLAST of Sgr025130 vs. NCBI nr
Match: KAE8645768.1 (hypothetical protein Csa_020499 [Cucumis sativus])

HSP 1 Score: 598.2 bits (1541), Expect = 8.7e-167
Identity = 359/644 (55.75%), Postives = 421/644 (65.37%), Query Frame = 0

Query: 23  VLIELTLTIAKFIVFLLFSKALVFVFFELELRSCSVVSDMFGILFGWRKASKCKKLIRRA 82
           +L+++ + +   I  L   K  VFV   L +   S    +  +   +   +  KKLI+ A
Sbjct: 185 LLVKVKVPVTVVIGSLSLKKVNVFVNCSLVVDKLSPNKKVEILSSNYTYGASFKKLIKTA 244

Query: 83  LCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAYDLIENFCEF 142
           LCRLK+L+KKR+SIV+ LREDL ELINNG+QQIAF RVEQLI DE+L+EAYDLIENFCE 
Sbjct: 245 LCRLKLLRKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLIQDETLMEAYDLIENFCEI 304

Query: 143 ILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQSFAMAAVEL 202
           ILV FSHIRKHKTCPDDI EAISSLIFASAR GDFPELK VRKLFEER+G+SF +AAVEL
Sbjct: 305 ILVKFSHIRKHKTCPDDITEAISSLIFASARFGDFPELKCVRKLFEERFGKSFIVAAVEL 364

Query: 203 CPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDWHQKQVLENGDQ 262
            PGNLVN QIKEKL+MQPV ++EKQRL++EIARDCFHPA+LALE CPDWH+KQVL+NGDQ
Sbjct: 365 SPGNLVNKQIKEKLVMQPVPNHEKQRLINEIARDCFHPAVLALENCPDWHKKQVLQNGDQ 424

Query: 263 THCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYLDCQSSPTLPDI 322
           T  + K E       +E+LER VI  DS+  +   ++D  CHSTSSS    QS P     
Sbjct: 425 TTVKGKVE-------SEELERVVISLDSTRDSNCGVLDSWCHSTSSSPSVSQSFP----- 484

Query: 323 TVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDASSPEFPPFSEE 382
                                                         NDAS+ EF PF EE
Sbjct: 485 ----------------------------------------------NDASTSEFLPFCEE 544

Query: 383 TIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMR-ETVQDGNDQSLIEHHDNSKKKL 442
            +VY DDVVEL +P TE GDL DQRFFKFKS +TS R E V DG+DQSLIE+H  S KK 
Sbjct: 545 AMVYFDDVVELSDPSTEYGDLLDQRFFKFKSLITSTREENVGDGDDQSLIENHYGSNKKA 604

Query: 443 VSERSCQSINGSPKRLRRRSTCR--ENNSLSHTKKKLMKCCCLSSHSCCLSSEMENYCLE 502
           VS RS Q INGSPK   RRS  R  EN+SL+HTKKKLMKC CLS HS  LSSE++NYC+E
Sbjct: 605 VSGRSNQIINGSPKEPSRRSMRREQENHSLNHTKKKLMKCYCLSCHS--LSSELQNYCVE 664

Query: 503 QPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLSGKDGHSHIDHISWTGDSD 562
           Q CYV+S+C  D S +  SE              N  D +LS K+         W  +S+
Sbjct: 665 QQCYVHSECRTDFSVIQSSEA-------------NNSDYDLSSKN--------LWNAESN 724

Query: 563 EEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKETNAKPKEFSTKGKHEPSLGFN 622
           EEIEF  F R    R+  IGTVVYD+FVYS CQP ENKETN K KE ST GKHEPS    
Sbjct: 725 EEIEFITFSRAKDQRNYGIGTVVYDVFVYSQCQPDENKETNTKLKELSTNGKHEPSKFTK 742

Query: 623 VMKNNFVKAADKYPSHVHPKLPDYDEITAKFIALKQEYLQRKQQ 664
            M     K ADKYPSHVHPKLP+Y+EI AKFI LK+EYL+R  +
Sbjct: 785 CM-----KGADKYPSHVHPKLPNYEEIAAKFICLKREYLERSMK 742

BLAST of Sgr025130 vs. NCBI nr
Match: KAA0031582.1 (Ist1 domain-containing protein [Cucumis melo var. makuwa] >TYK07034.1 Ist1 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 584.3 bits (1505), Expect = 1.3e-162
Identity = 346/592 (58.45%), Postives = 394/592 (66.55%), Query Frame = 0

Query: 76  KKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAYDL 135
           KKLI+ ALCRLK+LKKKR+SIV+ LREDL ELINNG+QQIAF RVEQL+ DE+L+E YDL
Sbjct: 4   KKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDL 63

Query: 136 IENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQSF 195
           IEN CEFILV FSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK VR LFEER+G+SF
Sbjct: 64  IENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSF 123

Query: 196 AMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDWHQKQ 255
           A+AAVEL PGNLVN QIKEKL+MQ VS++EKQRL++EIARDCF PA+LALEY PD  QKQ
Sbjct: 124 AVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQ 183

Query: 256 VLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYLDCQS 315
           VL N DQT  E K E       +E+LER VI  DS+  +   ++DP CHSTSSS   CQS
Sbjct: 184 VLPNEDQTTVEGKVE-------SEELERVVISLDSARDSNCGVLDPWCHSTSSSSSVCQS 243

Query: 316 SPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDASSPE 375
            P                                                   NDASS E
Sbjct: 244 FP---------------------------------------------------NDASSSE 303

Query: 376 FPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMR-ETVQDGNDQSLIEHH 435
           F PF EE IVY DDVVEL +P TE GDL DQRFFKFK  +TS R E V+D +DQSLIE H
Sbjct: 304 FLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIEKH 363

Query: 436 DNSKKKLVSERSCQSINGSPKRLRRRSTCR--ENNSLSHTKKKLMKCCCLSSHSCCLSSE 495
           D S KK VS RS Q ING PK   R+  CR  EN+SL+HTKKK  KCCCLS HS  LS E
Sbjct: 364 DASNKKSVSGRSNQIINGPPKEPSRKLMCREQENHSLNHTKKKFTKCCCLSCHS--LSFE 423

Query: 496 MENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLSGKDGHSHIDHI 555
           ++NY LEQ CYVYS+C  D              Y IRS   NRLD +LS K       H+
Sbjct: 424 LQNYSLEQQCYVYSECRTD-------------YYIIRSSEANRLDYDLSSK-------HL 483

Query: 556 SWTGDSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKETNAKPKEFSTKGKH 615
            W  +S+EEIEF  F R  + R+  IGTVVYD+FVYSHCQP ENKETN K +E      H
Sbjct: 484 -WNAESNEEIEFITFSRAKEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEE------H 503

Query: 616 EPSLGFNVMKNNFVKAADKYPSHVHPKLPDYDEITAKFIALKQEYLQRKQQQ 665
           EPS     M     K A KYPSHVHPKLPDYDEI  +FI+LK+EYLQR  ++
Sbjct: 544 EPSKFTKCM-----KGAHKYPSHVHPKLPDYDEIAVEFISLKREYLQRSMKR 503

BLAST of Sgr025130 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 9.7e-12
Identity = 50/161 (31.06%), Postives = 88/161 (54.66%), Query Frame = 0

Query: 74  KCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAY 133
           K K  ++ A+ R+++LK K+ +IVR  + ++ EL+   +++ A  RVE +I DE LIE +
Sbjct: 12  KLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRVETIIRDEYLIECF 71

Query: 134 DLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQ 193
            +IE  CE +    + I      P ++ E+I +L+++S R    PEL+ ++   + +YG+
Sbjct: 72  QIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPELEQIKNQLKAKYGK 131

Query: 194 SFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIA 235
                A   C  + VNP+I  KL       +   + +SEIA
Sbjct: 132 GLENEANCHCSTH-VNPKIVHKLSYATPDPSIIFQTLSEIA 170

BLAST of Sgr025130 vs. ExPASy Swiss-Prot
Match: Q3ZBV1 (IST1 homolog OS=Bos taurus OX=9913 GN=IST1 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-11
Identity = 47/170 (27.65%), Postives = 94/170 (55.29%), Query Frame = 0

Query: 71  KASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLI 130
           KA + +  +R  + RLK+L+KK+  + ++ R+++ + +  G  + A  RVE +I ++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 131 EAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKLVRKLFEE 190
           EA +++E +C+ +L  F  I+  K     + E++S+LI+A+ R   +  ELK+V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 191 RYGQSFAMAAVELCPGN---LVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           +Y + +     +LC  N    VN ++  KL ++       +R + EIA++
Sbjct: 127 KYSKEYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Sgr025130 vs. ExPASy Swiss-Prot
Match: P53990 (IST1 homolog OS=Homo sapiens OX=9606 GN=IST1 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-11
Identity = 47/170 (27.65%), Postives = 94/170 (55.29%), Query Frame = 0

Query: 71  KASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLI 130
           KA + +  +R  + RLK+L+KK+  + ++ R+++ + +  G  + A  RVE +I ++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 131 EAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKLVRKLFEE 190
           EA +++E +C+ +L  F  I+  K     + E++S+LI+A+ R   +  ELK+V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 191 RYGQSFAMAAVELCPGN---LVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           +Y + +     +LC  N    VN ++  KL ++       +R + EIA++
Sbjct: 127 KYSKEYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Sgr025130 vs. ExPASy Swiss-Prot
Match: Q9CX00 (IST1 homolog OS=Mus musculus OX=10090 GN=Ist1 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-11
Identity = 47/170 (27.65%), Postives = 94/170 (55.29%), Query Frame = 0

Query: 71  KASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLI 130
           KA + +  +R  + RLK+L+KK+  + ++ R+++ + +  G  + A  RVE +I ++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 131 EAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKLVRKLFEE 190
           EA +++E +C+ +L  F  I+  K     + E++S+LI+A+ R   +  ELK+V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 191 RYGQSFAMAAVELCPGN---LVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           +Y + +     +LC  N    VN ++  KL ++       +R + EIA++
Sbjct: 127 KYSKEYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Sgr025130 vs. ExPASy Swiss-Prot
Match: Q5R6G8 (IST1 homolog OS=Pongo abelii OX=9601 GN=IST1 PE=2 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 1.3e-11
Identity = 47/170 (27.65%), Postives = 94/170 (55.29%), Query Frame = 0

Query: 71  KASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLI 130
           KA + +  +R  + RLK+L+KK+  + ++ R+++ + +  G  + A  RVE +I ++ L+
Sbjct: 7   KAERLRVNLRLVINRLKLLEKKKTELAQKARKEIADYLAAGKDERARIRVEHIIREDYLV 66

Query: 131 EAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARC-GDFPELKLVRKLFEE 190
           EA +++E +C+ +L  F  I+  K     + E++S+LI+A+ R   +  ELK+V      
Sbjct: 67  EAMEILELYCDLLLARFGLIQSMKELDSGLAESVSTLIWAAPRLQSEVAELKIVADQLCA 126

Query: 191 RYGQSFAMAAVELCPGN---LVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           +Y + +     +LC  N    VN ++  KL ++       +R + EIA++
Sbjct: 127 KYSKGYG----KLCRTNQIGTVNDRLMHKLSVEAPPKILVERYLIEIAKN 172

BLAST of Sgr025130 vs. ExPASy TrEMBL
Match: A0A6J1D262 (uncharacterized protein LOC111016673 OS=Momordica charantia OX=3673 GN=LOC111016673 PE=3 SV=1)

HSP 1 Score: 710.3 bits (1832), Expect = 7.6e-201
Identity = 390/591 (65.99%), Postives = 434/591 (73.43%), Query Frame = 0

Query: 78  LIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAYDLIE 137
           +I+RAL RLKMLKKKR+SI+RQLREDL ELI+NG+QQIAF+RVEQLI DESL+EAYDLIE
Sbjct: 1   MIKRALSRLKMLKKKRYSILRQLREDLAELISNGYQQIAFNRVEQLIRDESLMEAYDLIE 60

Query: 138 NFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQSFAM 197
           NFCEFIL+NFSH+RKHKTCPDD+IEAISSLIFASARCGD PELK VRKLFEER+G+SFA 
Sbjct: 61  NFCEFILLNFSHVRKHKTCPDDVIEAISSLIFASARCGDLPELKSVRKLFEERFGRSFAA 120

Query: 198 AAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDWHQKQVL 257
            AVELCPGNLVNPQIKEKLL++PVSD+EKQ L+SEIARDCF PAILALEYCPDWHQKQVL
Sbjct: 121 TAVELCPGNLVNPQIKEKLLVKPVSDHEKQGLISEIARDCFRPAILALEYCPDWHQKQVL 180

Query: 258 ENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYLDCQSSP 317
           ENGDQTHCE KEE  +QDSNAE+ ER+V+C   S                          
Sbjct: 181 ENGDQTHCESKEEPNIQDSNAEEFERKVMCVSPS-------------------------- 240

Query: 318 TLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDASSPEFP 377
                      +EV S+S                       ATLE       DASSPE  
Sbjct: 241 -----------QEVCSSS-----------------------ATLE-------DASSPECS 300

Query: 378 PFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMRETVQDGNDQSLI-EHHDN 437
           PF EET+VYLDDVVELPNP  E+G LWDQR FKFKSPVT MRE VQ GN Q LI EHH+N
Sbjct: 301 PFCEETLVYLDDVVELPNPSMEEGYLWDQRLFKFKSPVTGMRENVQYGNGQGLIEEHHNN 360

Query: 438 SKKKLVSERSCQSINGSPKRLRRRSTCRENNSLSHTKKKLMKCCCLSSHSCCLSSEMENY 497
           SKK+ VSERS QS+N SPKR  RRST +E++SLSH KK+LMKCCCLS HS  L S MENY
Sbjct: 361 SKKRSVSERSSQSMNKSPKRSMRRSTHQEDDSLSHPKKRLMKCCCLSCHSSWLPSGMENY 420

Query: 498 CLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLSGKDGHSHIDHISWTG 557
           CLEQP YVY       SEV+ISEKKKV  +FI       LDN+LSGKD H          
Sbjct: 421 CLEQPSYVY-------SEVLISEKKKVDPHFI-------LDNSLSGKDRH---------- 480

Query: 558 DSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKETNAKPKEFSTKGKHEPSL 617
                +EFD  PRT KIR+CE+GT+VYD+FVYSHCQP ENKETNAKP+E STK KHE SL
Sbjct: 481 ----RVEFDTLPRTEKIRNCEVGTIVYDVFVYSHCQPVENKETNAKPEEISTKCKHESSL 496

Query: 618 GFNVMKNNF---VKAADKYPSHVHPKLPDYDEITAKFIALKQEYLQRKQQQ 665
           GFN MK++F    KAADKYPSHVHPKLPDYDEI AKFIALK+EY QRKQ Q
Sbjct: 541 GFNGMKSSFTKCTKAADKYPSHVHPKLPDYDEIAAKFIALKREYSQRKQHQ 496

BLAST of Sgr025130 vs. ExPASy TrEMBL
Match: A0A1S4E0G2 (uncharacterized protein LOC103495691 OS=Cucumis melo OX=3656 GN=LOC103495691 PE=3 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 7.4e-172
Identity = 359/606 (59.24%), Postives = 408/606 (67.33%), Query Frame = 0

Query: 62  MFGILFGWRKASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVE 121
           MFG LFGWRKASKCKKLI+ ALCRLK+LKKKR+SIV+ LREDL ELINNG+QQIAF RVE
Sbjct: 1   MFGFLFGWRKASKCKKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVE 60

Query: 122 QLIGDESLIEAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 181
           QL+ DE+L+E YDLIEN CEFILV FSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK
Sbjct: 61  QLLQDETLMEVYDLIENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELK 120

Query: 182 LVRKLFEERYGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPA 241
            VR LFEER+G+SFA+AAVEL PGNLVN QIKEKL+MQ VS++EKQRL++EIARDCF PA
Sbjct: 121 CVRMLFEERFGKSFAVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPA 180

Query: 242 ILALEYCPDWHQKQVLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDP 301
           +LALEY PD  QKQVL+N DQT  E K E       +E+LER VI  DS+  +   ++DP
Sbjct: 181 VLALEYRPDSDQKQVLQNEDQTTVEGKVE-------SEELERVVISLDSARDSNCGVLDP 240

Query: 302 QCHSTSSSYLDCQSSPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATL 361
            CHSTSSS   CQS P                                            
Sbjct: 241 WCHSTSSSSSVCQSFP-------------------------------------------- 300

Query: 362 EDGLDRDNDASSPEFPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMR-E 421
                  NDASS EF PF EE IVY DDVVEL +P TE GDL DQRFFKFK  +TS R E
Sbjct: 301 -------NDASSSEFLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREE 360

Query: 422 TVQDGNDQSLIEHHDNSKKKLVSERSCQSINGSPKRLRRRSTCR--ENNSLSHTKKKLMK 481
            V+D +DQSLIE HD S KK VS RS Q ING PK   R+  CR  EN+SL+HTKKK  K
Sbjct: 361 NVRDRDDQSLIEKHDASNKKSVSGRSNQIINGPPKEPSRKFMCREQENHSLNHTKKKFTK 420

Query: 482 CCCLSSHSCCLSSEMENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDN 541
           CCCLS HS  LS E++NY LEQ CYVYS+C  D              Y IRS   NRLD 
Sbjct: 421 CCCLSCHS--LSFELQNYSLEQQCYVYSECRTD-------------YYIIRSSEANRLDY 480

Query: 542 NLSGKDGHSHIDHISWTGDSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKE 601
           +LS K       H+ W  +S+EEIEF  F R  + R+  IGTVVYD+FVYSHCQP ENKE
Sbjct: 481 DLSSK-------HL-WNAESNEEIEFITFSRAKEQRNYGIGTVVYDVFVYSHCQPDENKE 514

Query: 602 TNAKPKEFSTKGKHEPSLGFNVMKNNFVKAADKYPSHVHPKLPDYDEITAKFIALKQEYL 661
           TN K +E      HEPS     M     K A KYPSHVHPKLPDYDEI  +FI+LK+EYL
Sbjct: 541 TNTKLEE------HEPSKFTKCM-----KGAHKYPSHVHPKLPDYDEIAVEFISLKREYL 514

Query: 662 QRKQQQ 665
           QR  ++
Sbjct: 601 QRSMKR 514

BLAST of Sgr025130 vs. ExPASy TrEMBL
Match: A0A0A0K3R2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G047390 PE=3 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 5.0e-168
Identity = 350/591 (59.22%), Postives = 401/591 (67.85%), Query Frame = 0

Query: 76  KKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAYDL 135
           KKLI+ ALCRLK+L+KKR+SIV+ LREDL ELINNG+QQIAF RVEQLI DE+L+EAYDL
Sbjct: 20  KKLIKTALCRLKLLRKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLIQDETLMEAYDL 79

Query: 136 IENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQSF 195
           IENFCE ILV FSHIRKHKTCPDDI EAISSLIFASAR GDFPELK VRKLFEER+G+SF
Sbjct: 80  IENFCEIILVKFSHIRKHKTCPDDITEAISSLIFASARFGDFPELKCVRKLFEERFGKSF 139

Query: 196 AMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDWHQKQ 255
            +AAVEL PGNLVN QIKEKL+MQPV ++EKQRL++EIARDCFHPA+LALE CPDWH+KQ
Sbjct: 140 IVAAVELSPGNLVNKQIKEKLVMQPVPNHEKQRLINEIARDCFHPAVLALENCPDWHKKQ 199

Query: 256 VLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYLDCQS 315
           VL+NGDQT  + K E       +E+LER VI  DS+  +   ++D  CHSTSSS    QS
Sbjct: 200 VLQNGDQTTVKGKVE-------SEELERVVISLDSTRDSNCGVLDSWCHSTSSSPSVSQS 259

Query: 316 SPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDASSPE 375
            P                                                   NDAS+ E
Sbjct: 260 FP---------------------------------------------------NDASTSE 319

Query: 376 FPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMR-ETVQDGNDQSLIEHH 435
           F PF EE +VY DDVVEL +P TE GDL DQRFFKFKS +TS R E V DG+DQSLIE+H
Sbjct: 320 FLPFCEEAMVYFDDVVELSDPSTEYGDLLDQRFFKFKSLITSTREENVGDGDDQSLIENH 379

Query: 436 DNSKKKLVSERSCQSINGSPKRLRRRSTCR--ENNSLSHTKKKLMKCCCLSSHSCCLSSE 495
             S KK VS RS Q INGSPK   RRS  R  EN+SL+HTKKKLMKC CLS HS  LSSE
Sbjct: 380 YGSNKKAVSGRSNQIINGSPKEPSRRSMRREQENHSLNHTKKKLMKCYCLSCHS--LSSE 439

Query: 496 MENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLSGKDGHSHIDHI 555
           ++NYC+EQ CYV+S+C  D S +  SE              N  D +LS K+        
Sbjct: 440 LQNYCVEQQCYVHSECRTDFSVIQSSEA-------------NNSDYDLSSKN-------- 499

Query: 556 SWTGDSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKETNAKPKEFSTKGKH 615
            W  +S+EEIEF  F R    R+  IGTVVYD+FVYS CQP ENKETN K KE ST GKH
Sbjct: 500 LWNAESNEEIEFITFSRAKDQRNYGIGTVVYDVFVYSQCQPDENKETNTKLKELSTNGKH 524

Query: 616 EPSLGFNVMKNNFVKAADKYPSHVHPKLPDYDEITAKFIALKQEYLQRKQQ 664
           EPS     M     K ADKYPSHVHPKLP+Y+EI AKFI LK+EYL+R  +
Sbjct: 560 EPSKFTKCM-----KGADKYPSHVHPKLPNYEEIAAKFICLKREYLERSMK 524

BLAST of Sgr025130 vs. ExPASy TrEMBL
Match: A0A5D3C6Z3 (Ist1 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003520 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 6.3e-163
Identity = 346/592 (58.45%), Postives = 394/592 (66.55%), Query Frame = 0

Query: 76  KKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEAYDL 135
           KKLI+ ALCRLK+LKKKR+SIV+ LREDL ELINNG+QQIAF RVEQL+ DE+L+E YDL
Sbjct: 4   KKLIKTALCRLKLLKKKRYSIVKLLREDLCELINNGYQQIAFKRVEQLLQDETLMEVYDL 63

Query: 136 IENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYGQSF 195
           IEN CEFILV FSH+RKHKTCPDD+IEAISSLIFASAR GDFPELK VR LFEER+G+SF
Sbjct: 64  IENLCEFILVKFSHVRKHKTCPDDVIEAISSLIFASARFGDFPELKCVRMLFEERFGKSF 123

Query: 196 AMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDWHQKQ 255
           A+AAVEL PGNLVN QIKEKL+MQ VS++EKQRL++EIARDCF PA+LALEY PD  QKQ
Sbjct: 124 AVAAVELSPGNLVNKQIKEKLVMQSVSNHEKQRLINEIARDCFCPAVLALEYRPDSDQKQ 183

Query: 256 VLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYLDCQS 315
           VL N DQT  E K E       +E+LER VI  DS+  +   ++DP CHSTSSS   CQS
Sbjct: 184 VLPNEDQTTVEGKVE-------SEELERVVISLDSARDSNCGVLDPWCHSTSSSSSVCQS 243

Query: 316 SPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDASSPE 375
            P                                                   NDASS E
Sbjct: 244 FP---------------------------------------------------NDASSSE 303

Query: 376 FPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFKFKSPVTSMR-ETVQDGNDQSLIEHH 435
           F PF EE IVY DDVVEL +P TE GDL DQRFFKFK  +TS R E V+D +DQSLIE H
Sbjct: 304 FLPFCEEAIVYFDDVVELSDPSTEFGDLLDQRFFKFKPHITSTREENVRDRDDQSLIEKH 363

Query: 436 DNSKKKLVSERSCQSINGSPKRLRRRSTCR--ENNSLSHTKKKLMKCCCLSSHSCCLSSE 495
           D S KK VS RS Q ING PK   R+  CR  EN+SL+HTKKK  KCCCLS HS  LS E
Sbjct: 364 DASNKKSVSGRSNQIINGPPKEPSRKLMCREQENHSLNHTKKKFTKCCCLSCHS--LSFE 423

Query: 496 MENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLSGKDGHSHIDHI 555
           ++NY LEQ CYVYS+C  D              Y IRS   NRLD +LS K       H+
Sbjct: 424 LQNYSLEQQCYVYSECRTD-------------YYIIRSSEANRLDYDLSSK-------HL 483

Query: 556 SWTGDSDEEIEFDMFPRTWKIRSCEIGTVVYDIFVYSHCQPYENKETNAKPKEFSTKGKH 615
            W  +S+EEIEF  F R  + R+  IGTVVYD+FVYSHCQP ENKETN K +E      H
Sbjct: 484 -WNAESNEEIEFITFSRAKEQRNYGIGTVVYDVFVYSHCQPDENKETNTKLEE------H 503

Query: 616 EPSLGFNVMKNNFVKAADKYPSHVHPKLPDYDEITAKFIALKQEYLQRKQQQ 665
           EPS     M     K A KYPSHVHPKLPDYDEI  +FI+LK+EYLQR  ++
Sbjct: 544 EPSKFTKCM-----KGAHKYPSHVHPKLPDYDEIAVEFISLKREYLQRSMKR 503

BLAST of Sgr025130 vs. ExPASy TrEMBL
Match: A0A2N9EJ18 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6798 PE=3 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 5.4e-106
Identity = 277/694 (39.91%), Postives = 371/694 (53.46%), Query Frame = 0

Query: 62  MFGILFGWRKASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVE 121
           MF ILFGWRKASKCKKLI+R  CRLK++K KR+SIVR +RED+ +LI +GH+QIAF+RVE
Sbjct: 1   MFDILFGWRKASKCKKLIKRVQCRLKLMKIKRNSIVRLIREDVAQLIKSGHEQIAFNRVE 60

Query: 122 QLIGDESLIEAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 181
           QLI DES++  YDL+++FCEFILVNFS+IR+HK CP+DI EA+SSLIFASARCGD PEL+
Sbjct: 61  QLIKDESIVAVYDLLDHFCEFILVNFSYIRRHKDCPNDINEAVSSLIFASARCGDLPELR 120

Query: 182 LVRKLFEERYGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARD-CFHP 241
           ++RKLF ERYGQ FA  AVEL  GNLVN +IKEKL  + V+D+ K RL+ EIARD    P
Sbjct: 121 VIRKLFGERYGQGFARTAVELSLGNLVNREIKEKLSPKSVTDDVKHRLVDEIARDYSLIP 180

Query: 242 AILALEYCPDWHQ----------KQVLENGDQTHCEIKEETKVQDSNAEDLEREVICADS 301
            ILALEY P+W Q          +QV     QT+ +  + +++Q S  E++ER VI  D 
Sbjct: 181 EILALEYYPEWKQEQEQVKESSGQQVPNTDVQTYNDTTKGSEMQVSTVEEMERNVIYVD- 240

Query: 302 SWGNGSDIVDPQCHSTSSSYLDCQSSPTLPDITVPNGREEVSSNSALACSDLLSWKSKEC 361
           S      I+   C S   S+ D   S       V      V  +     +D +  KSK  
Sbjct: 241 SLSTSKKILTELCDSL--SHQDSDISIAFTSSIVQQSSTSVVESPMNNKADKVE-KSKLN 300

Query: 362 KCRGWIAAATLEDGLDRDNDASSPEFPPFSEETIVYLDDVVELPNPPTEDGDLWDQRFFK 421
               +  +      + +   +SS   P F EETIVYLDD+ E P+  T + D  DQR FK
Sbjct: 301 SPYKYKISGLDHKEVRKAAASSSESLPQFPEETIVYLDDIEEFPSSSTNERDCQDQRLFK 360

Query: 422 FKSPVTSMRETVQDGNDQSLIEHHDNSKKKLVSERSCQSINGSPKRLRRRSTCRENNSLS 481
           FKS +    ET++D  DQS + H+++  +K  S  S +    S KRLRRRS  ++N S++
Sbjct: 361 FKSTLLPKSETIEDSGDQSNMNHYESWSEKSGSRSSRKRRKASGKRLRRRSMSQDNQSVN 420

Query: 482 -------------------HTK-----------------KKLMKCCCLSS----HSCCLS 541
                              HTK                 K+L K CCL S     SC  S
Sbjct: 421 DIECGLYNDNLCNNSPTHRHTKHQKQTRGVGRQQSNYAPKRLKKPCCLDSEINFQSCNCS 480

Query: 542 ---------SEMENYCLEQPCYVYSDCNKDDSEVVISEKKKVGSYFIRSPAHNRLDNNLS 601
                    SE E+ CL  P Y  +  NKD  EV+  ++K+  +  +  P H      + 
Sbjct: 481 YDPEGNFFNSETESSCLGNPIYFCTCDNKDSREVLPCKQKRGITTLVGFPTHGPEQELVC 540

Query: 602 GKDGHSHIDHISWTGDSDEEIEF-DMFPRTWKIRSCEIGTVVYDIFVYSHCQP-YENKET 661
            K  H    + SW  + D+E+E+     +  + RS + G+ VY++F Y   QP  +NKE 
Sbjct: 541 NKCCH---HYWSWNEELDKEMEWTTSTQKPRRRRSHDSGSTVYNVFTYLDHQPNKQNKEM 600

BLAST of Sgr025130 vs. TAIR 10
Match: AT2G14830.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-46
Identity = 98/218 (44.95%), Postives = 144/218 (66.06%), Query Frame = 0

Query: 62  MFGILFGWRKASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVE 121
           MF  LFGWR+ SKCK ++++  CRL +LK K+++I   LR D+ +L+  G +  A  R +
Sbjct: 71  MFCFLFGWRRTSKCKSVVKQLQCRLNLLKNKKYAISSHLRNDIAQLLRIGERDRALHRAQ 130

Query: 122 QLIGDESLIEAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELK 181
           QL  DE+L+  Y L+ +F + IL+N S+IR+ +  PD I EA+S+L+FASARCGD PEL+
Sbjct: 131 QLFLDENLMSLYHLLLHFSDIILLNLSYIRRRRDLPDGINEAVSTLVFASARCGDLPELR 190

Query: 182 LVRKLFEERYGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDC-FHP 241
            +R LF +RYG  F   A+ L PGN VNPQ+ EKL +  VSD+ K +L+ EI  +     
Sbjct: 191 ALRVLFGKRYGNHFVDTALNLLPGNCVNPQVIEKLSIISVSDDAKSKLLGEIVEEYNLRL 250

Query: 242 AILALEYCPDWHQKQVLENGDQTHCEIKEETKVQDSNA 279
            +LA+EY P++H KQVL++      + +EE +V  SN+
Sbjct: 251 EVLAIEYTPEFH-KQVLKSE-----KAEEEKEVMSSNS 282

HSP 2 Score: 40.4 bits (93), Expect = 6.5e-03
Identity = 17/32 (53.12%), Postives = 24/32 (75.00%), Query Frame = 0

Query: 631 KYPSHVHPKLPDYDEITAKFIALKQEYLQRKQ 663
           K   HVHPKLPDYD+I A F AL+++  Q+++
Sbjct: 419 KKAMHVHPKLPDYDQIVAHFTALRKQQQQKQK 450

BLAST of Sgr025130 vs. TAIR 10
Match: AT1G34220.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 122.9 bits (307), Expect = 9.9e-28
Identity = 63/166 (37.95%), Postives = 104/166 (62.65%), Query Frame = 0

Query: 71  KASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLI 130
           KA+KCK L++  + R+K+++ +R + ++Q+R ++ +L+  G +  A  RVE +I +E ++
Sbjct: 13  KAAKCKTLLKLTIPRIKLIRNRREAQIKQMRREIAKLLETGQEATARIRVEHIIREEKMM 72

Query: 131 EAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEER 190
            A +++E FCE I V    I   + CP D+ EAISS+ FA+ RC D  EL+ V+ LF  +
Sbjct: 73  AAQEILELFCELIAVRLPIIEAQRECPLDLKEAISSVCFAAPRCSDLTELQQVQILFVSK 132

Query: 191 YGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           YG+ F  AA EL P + VN ++ E L ++  S   K +L+ EIA +
Sbjct: 133 YGKEFVAAASELKPDSGVNRKLVELLSVRAPSPETKLKLLKEIAEE 178

BLAST of Sgr025130 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 117.5 bits (293), Expect = 4.2e-26
Identity = 94/312 (30.13%), Postives = 154/312 (49.36%), Query Frame = 0

Query: 72  ASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIE 131
           +SKCK   + A+ R+K+++ KR  +V+Q+R D+  L+ +G    A  RVE +I ++++  
Sbjct: 28  SSKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQA 87

Query: 132 AYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERY 191
           A ++IE FCE I+   + I K K CP D+ E I+SLIFA+ RC + PEL  +R +F ++Y
Sbjct: 88  ANEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKY 147

Query: 192 GQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARDCFHPAILALEYCPDW 251
           G+ F  AA +L P   VN  + +KL ++      K ++M EIA+          E+  DW
Sbjct: 148 GKDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAK----------EFQVDW 207

Query: 252 HQKQVLENGDQTHCEIKEETKVQDSNAEDLEREVICADSSWGNGSDIVDPQCHSTSSSYL 311
                    D T  E +E  K Q+ +  D  R+ + A S   N + I             
Sbjct: 208 ---------DTTETE-QELLKPQEESI-DGPRKFVSASSLPVNRAAI------------- 267

Query: 312 DCQSSPTLPDITVPNGREEVSSNSALACSDLLSWKSKECKCRGWIAAATLEDGLDRDNDA 371
              + P  P   VP     +S N+    ++  +  + E   +  +AAA +   L    D+
Sbjct: 268 ---NEPIDPTKAVPRSTSSMSINTHYHDTESAAEAATEL-AKQAVAAAQVASLLATRRDS 301

Query: 372 SSPEFPPFSEET 384
           S+ EF   S+ +
Sbjct: 328 SNKEFSVSSDHS 301

BLAST of Sgr025130 vs. TAIR 10
Match: AT1G25420.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 116.7 bits (291), Expect = 7.1e-26
Identity = 60/164 (36.59%), Postives = 100/164 (60.98%), Query Frame = 0

Query: 73  SKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLIEA 132
           +KCK  +  A+ R+K+L+ KR   ++ +++++   +  G + IA  RVE +I + +L  A
Sbjct: 15  AKCKTSLNLAIARMKLLQNKRDMQLKHMKKEIAHFLQAGQEPIARIRVEHVIREMNLWAA 74

Query: 133 YDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEERYG 192
           Y+++E FCEFIL     +   K CP ++ EAI+S+IFA+ RC + P+L  ++ LF  +YG
Sbjct: 75  YEILELFCEFILARVPILESEKECPRELREAIASIIFAAPRCSEVPDLLQIKNLFGTKYG 134

Query: 193 QSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           + F M A EL P + VN  I EKL     S   + +++ EIA++
Sbjct: 135 KEFIMVASELRPDSGVNRTIIEKLSPTSPSGAARLKMLKEIAQE 178

BLAST of Sgr025130 vs. TAIR 10
Match: AT2G19710.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 116.3 bits (290), Expect = 9.3e-26
Identity = 62/166 (37.35%), Postives = 99/166 (59.64%), Query Frame = 0

Query: 71  KASKCKKLIRRALCRLKMLKKKRHSIVRQLREDLDELINNGHQQIAFDRVEQLIGDESLI 130
           K +KCK  ++ A  RLK+LK K+   ++QLR +L +L+ +G    A  RVE ++ +E  +
Sbjct: 10  KPAKCKTALQMANSRLKILKNKKEIQIKQLRRELAQLLESGQTPTARIRVEHVVREEKTV 69

Query: 131 EAYDLIENFCEFILVNFSHIRKHKTCPDDIIEAISSLIFASARCGDFPELKLVRKLFEER 190
            AY+LI  +CE ++V    I   K CP D+ EA++S++FAS R  D PEL  + K F  +
Sbjct: 70  AAYELIGIYCELLVVRLGVIESQKNCPIDLKEAVTSVLFASQRLSDVPELSEIFKQFTTK 129

Query: 191 YGQSFAMAAVELCPGNLVNPQIKEKLLMQPVSDNEKQRLMSEIARD 237
           YG+ F+ +AVEL P + V+  + EKL  +      K +++  IA +
Sbjct: 130 YGKDFSTSAVELRPDSGVSRLLVEKLSAKAPDGPTKVKILMAIAEE 175