Sgr024654 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr024654
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionphosphoenolpyruvate carboxylase kinase 1-like
Locationtig00002486: 1577943 .. 1579098 (+)
RNA-Seq ExpressionSgr024654
SyntenySgr024654
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGACGCATTGAGGAGAGACTATCAAGTAAATGAAGAGATCGGCCGGGGACGATTTGGCACCGTTTTCCGATGCACTTCCAGAGCGGCCGGTGGATTCTTCGCCGTCAAAACTATCGACAAGCGGCGTATCTCGCCGGTGATTCACTTGACGCCCAATGCCTCGTTAACGAGACCAAGATCCTCCATCTTCTCTACCCTCACCCTCACGTTCTTGCGCTCCACAATCTTTACGAAGACGAATTTCACCTCCACATGGTCCTCGACCTCTGCTCTTCTTCCGATCTCCACCGCGAGTCACTCTCCAGGTTCTGTCGAGGTGCAGGCCGCTTCGATTATGTCGCAGCTCATGCACGCCGTGGCTCATTGTCACCGTCTCGGCGTCGCTCACCGGGATATCAAGCCGGACAACATTCTCTTCGACGAATGGGATTCTGTGAAACTAGCGGATTTCGGATCGGCGGAGATGTTCAAGCAGGGCCAAGAGTCGATGAGCGGCATCGTCGGAACGCCGTACTACGTAGCGCCTGAAGTGCTGGCCGGGAAGGACTACGGGGAGAAGGTTGACGTCTGGAGCGCCGGCGTCGTTTTGTACGTTATGTTAGCAGGTTTTCCACCGTTTCACGGAGAGTCGGTGGTTGAGATCTTCCAGGCGGTGTTGCGAGCGAATTTGAGATTTCCTACCAGAGTTTTTCACTCCGTATCTCCCGCGGCTAAAGACTTGCTCCGGAAGATGCTTTGTAAAGATGTTTCCAGAAGATTCTCCGCTGTACAAGTCCTGAGTAAGTATCTAATTACGTTTCCGTTCGATCAATATACCTGAGATTTGCGCTTAATTTTTCTTTTCCATGTTCTTTAATCAATCTTCTGAGGAAGATTATATTGTTCGTAACGACAATTAAACTTTCTTACAGAAAATAACAGAAACACGCACACCTAATTATGCCCTAGAACTGAAATTTCGTTCAATTTCTTGAATCTTTGAAATCTTATCAATGGATTCAAACACACATTACAAACAAATCCATGGCTTAATTTTCTTTTACACCAATCTTCTTCTTCTTTCTTGTAATTTTTTGTTTGGATTGGCGGAAGCAGGGCACCCATGGATTACCAGCTGCGGAGGAACCAGGCCGGCGACGGAGTTAGCCTGA

mRNA sequence

ATGAGCGACGCATTGAGGAGAGACTATCAAGTAAATGAAGAGATCGGCCGGGGACGATTTGGCACCGTTTTCCGATGCACTTCCAGAGCGGCCGGTGGATTCTTCGCCGTCAAAACTATCGACAAGCGGCGTATCTCGCCGGTGATTCACTTGACGCCCAATGCCTCGTTAACGAGACCAAGATCCTCCATCTTCTCTACCCTCACCCTCACGTTCTTGCGCTCCACAATCTTTACGAAGACGAATTTCACCTCCACATGGTCCTCGACCTCTGCTCTTCTTCCGATCTCCACCGCGAGTCACTCTCCAGGTTCTGTCGAGGTGCAGGCCGCTTCGATTATGTCGCAGCTCATGCACGCCGTGGCTCATTGTCACCGTCTCGGCGTCGCTCACCGGGATATCAAGCCGGACAACATTCTCTTCGACGAATGGGATTCTGTGAAACTAGCGGATTTCGGATCGGCGGAGATGTTCAAGCAGGGCCAAGAGTCGATGAGCGGCATCGTCGGAACGCCGTACTACGTAGCGCCTGAAGTGCTGGCCGGGAAGGACTACGGGGAGAAGGTTGACGTCTGGAGCGCCGGCGTCGTTTTGTACGTTATGTTAGCAGGTTTTCCACCGTTTCACGGAGAGTCGGTGGTTGAGATCTTCCAGGCGGTGTTGCGAGCGAATTTGAGATTTCCTACCAGAGTTTTTCACTCCGTATCTCCCGCGGCTAAAGACTTGCTCCGGAAGATGCTTTGTAAAGATGTTTCCAGAAGATTCTCCGCTGTACAAGTCCTGAGGCACCCATGGATTACCAGCTGCGGAGGAACCAGGCCGGCGACGGAGTTAGCCTGA

Coding sequence (CDS)

ATGAGCGACGCATTGAGGAGAGACTATCAAGTAAATGAAGAGATCGGCCGGGGACGATTTGGCACCGTTTTCCGATGCACTTCCAGAGCGGCCGGTGGATTCTTCGCCGTCAAAACTATCGACAAGCGGCGTATCTCGCCGGTGATTCACTTGACGCCCAATGCCTCGTTAACGAGACCAAGATCCTCCATCTTCTCTACCCTCACCCTCACGTTCTTGCGCTCCACAATCTTTACGAAGACGAATTTCACCTCCACATGGTCCTCGACCTCTGCTCTTCTTCCGATCTCCACCGCGAGTCACTCTCCAGGTTCTGTCGAGGTGCAGGCCGCTTCGATTATGTCGCAGCTCATGCACGCCGTGGCTCATTGTCACCGTCTCGGCGTCGCTCACCGGGATATCAAGCCGGACAACATTCTCTTCGACGAATGGGATTCTGTGAAACTAGCGGATTTCGGATCGGCGGAGATGTTCAAGCAGGGCCAAGAGTCGATGAGCGGCATCGTCGGAACGCCGTACTACGTAGCGCCTGAAGTGCTGGCCGGGAAGGACTACGGGGAGAAGGTTGACGTCTGGAGCGCCGGCGTCGTTTTGTACGTTATGTTAGCAGGTTTTCCACCGTTTCACGGAGAGTCGGTGGTTGAGATCTTCCAGGCGGTGTTGCGAGCGAATTTGAGATTTCCTACCAGAGTTTTTCACTCCGTATCTCCCGCGGCTAAAGACTTGCTCCGGAAGATGCTTTGTAAAGATGTTTCCAGAAGATTCTCCGCTGTACAAGTCCTGAGGCACCCATGGATTACCAGCTGCGGAGGAACCAGGCCGGCGACGGAGTTAGCCTGA

Protein sequence

MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSVEVQAASIMSQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATELA
Homology
BLAST of Sgr024654 vs. NCBI nr
Match: XP_022146428.1 (phosphoenolpyruvate carboxylase kinase 1-like [Momordica charantia])

HSP 1 Score: 390.2 bits (1001), Expect = 1.5e-104
Identity = 211/284 (74.30%), Postives = 227/284 (79.93%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASL--T 60
           MSDAL+RDYQV EEIGRGRFGTVFRCTSRA+G F+AVKTID+RRIS    L     +  T
Sbjct: 1   MSDALKRDYQVIEEIGRGRFGTVFRCTSRASGEFYAVKTIDRRRISAGDSLDAECLVNET 60

Query: 61  RPRSSIFS---TLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSVEVQAASIMS 120
           +    ++S    L L  L    F         SS+     I+    S    E QAASIMS
Sbjct: 61  KILHLLYSHPHILALHDLYEDEFHLHMVLDLCSSSDLHTRITLQVFS----EAQAASIMS 120

Query: 121 QLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYYV 180
           QLM A+AHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMF+QG+ESM+G+VGTPYYV
Sbjct: 121 QLMQALAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFRQGEESMNGVVGTPYYV 180

Query: 181 APEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSV 240
           APEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESV EIF AVLRANLRFP+RVFHSV
Sbjct: 181 APEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVAEIFHAVLRANLRFPSRVFHSV 240

Query: 241 SPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATELA 280
           SPAAKDLLRKMLCKDV RRFSA QVLRHPWITSCGG RPATELA
Sbjct: 241 SPAAKDLLRKMLCKDVFRRFSAEQVLRHPWITSCGGARPATELA 280

BLAST of Sgr024654 vs. NCBI nr
Match: XP_038900016.1 (phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida])

HSP 1 Score: 379.0 bits (972), Expect = 3.5e-101
Identity = 201/284 (70.77%), Postives = 219/284 (77.11%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MSD  +RDYQV EEIGRGRFGTVFRCTSR++GG +AVK IDKRRIS    L     +   
Sbjct: 1   MSDGFKRDYQVIEEIGRGRFGTVFRCTSRSSGGIYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      + L +    I    N     S    +L + ++S     +      EV+AA+IM
Sbjct: 61  K-----IIHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLRVFSEVEAATIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFK G+ESMSG+VGTPYY
Sbjct: 121 SQLMHAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKHGEESMSGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGK YGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKKYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSP AKDLLRKMLCKDVSRR SA QVLRHPWITSC   RPATEL
Sbjct: 241 VSPPAKDLLRKMLCKDVSRRISAEQVLRHPWITSCVENRPATEL 279

BLAST of Sgr024654 vs. NCBI nr
Match: XP_022924623.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata])

HSP 1 Score: 372.1 bits (954), Expect = 4.2e-99
Identity = 201/284 (70.77%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MSD L+RDYQV EEIGRGRFGTVFRCTSR++G  +AVKTIDKRRI     L     +   
Sbjct: 1   MSDDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N          +L + ++S     V      E +AA IM
Sbjct: 61  K-----ILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM G+VGTPYY
Sbjct: 121 SQLMHAVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSPAAKDLLRKMLCKDVSRR SA QVLRHPWITSC  +R AT L
Sbjct: 241 VSPAAKDLLRKMLCKDVSRRISAEQVLRHPWITSCVESRAATAL 279

BLAST of Sgr024654 vs. NCBI nr
Match: KAG6582384.1 (Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018790.1 Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 370.2 bits (949), Expect = 1.6e-98
Identity = 200/284 (70.42%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MS+ L+RDYQV EEIGRGRFGTVFRCTSR++G  +AVKTIDKRRI     L     +   
Sbjct: 47  MSNDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 106

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N          +L + ++S     V      E +AA IM
Sbjct: 107 K-----ILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIM 166

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM G+VGTPYY
Sbjct: 167 SQLMHAVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYY 226

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 227 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 286

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSPAAKDLLRKMLCKDVSRR SA QVLRHPWITSC  +R AT L
Sbjct: 287 VSPAAKDLLRKMLCKDVSRRISAEQVLRHPWITSCVESRAATAL 325

BLAST of Sgr024654 vs. NCBI nr
Match: XP_023526782.1 (phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.8 bits (948), Expect = 2.1e-98
Identity = 200/284 (70.42%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MS+ L+RDYQV EEIGRGRFGTVFRCTSR++G  +AVKTIDKRRI     L     +   
Sbjct: 1   MSNDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N          +L + ++S     V      E +AA IM
Sbjct: 61  K-----ILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM G+VGTPYY
Sbjct: 121 SQLMHAVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSPAAKDLLRKMLCKDVSRR SA QVLRHPWITSC  +R AT L
Sbjct: 241 VSPAAKDLLRKMLCKDVSRRISAEQVLRHPWITSCVESRAATGL 279

BLAST of Sgr024654 vs. ExPASy Swiss-Prot
Match: Q9SPK4 (Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK1 PE=1 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 2.1e-64
Identity = 138/266 (51.88%), Postives = 170/266 (63.91%), Query Frame = 0

Query: 9   YQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSIFSTL 68
           YQ+ EEIGRGRFGTV R  + A G FFA KTIDK  +S  +      +  +  + +    
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSYHP 74

Query: 69  TLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGS-VEVQAASIMSQLMHAVAHCHRL 128
            +  +   I T +  +         + I     S G+  E Q AS   Q++ A++HCHR 
Sbjct: 75  NIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCHRY 134

Query: 129 GVAHRDIKPDNILFD-EWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEVLAGKDYG 188
           GV HRDIKP+NIL D   D+VK+ DFGS     +G E+  G+VGTPYYVAPEVL G  YG
Sbjct: 135 GVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYSYG 194

Query: 189 EKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAAKDLLRKM 248
           EKVD+WSAGVVLY MLAG PPF+GE+  EIF+AVLR NLRFPT++F  VS  AKD LRK+
Sbjct: 195 EKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRKL 254

Query: 249 LCKDVSRRFSAVQVLRHPWITSCGGT 273
           +CKD SRRFSA Q LRHPWI   G T
Sbjct: 255 ICKDASRRFSAEQALRHPWIQRAGET 279

BLAST of Sgr024654 vs. ExPASy Swiss-Prot
Match: Q93VK0 (Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK2 PE=1 SV=2)

HSP 1 Score: 228.4 bits (581), Expect = 9.9e-59
Identity = 132/271 (48.71%), Postives = 167/271 (61.62%), Query Frame = 0

Query: 5   LRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSI 64
           L  +YQ+ +EIGRGRFGT+ RC S A   F+A KTIDKR +  +  L      T PR   
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVL--IDALDRECIETEPRIMA 66

Query: 65  FSTLTLTFLR--STIFTKTNFTSTWSSTSALLPISTASHSPGS--VEVQAASIMSQLMHA 124
                   +R      T+ +           + I     S G    E ++AS   Q++ A
Sbjct: 67  MLPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSA 126

Query: 125 VAHCHRLGVAHRDIKPDNILFD-EWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEV 184
           +AHCHR  V HRD+KPDN+L D     VKL DFGSA     G E+  G+VGTPYYVAPEV
Sbjct: 127 LAHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEV 186

Query: 185 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAA 244
           + G+ Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF+++LR NLRFP + F SVS  A
Sbjct: 187 VMGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEA 246

Query: 245 KDLLRKMLCKDVSRRFSAVQVLRHPWITSCG 271
           KDLLRKM+C+DVSRRFSA   LRH W+ + G
Sbjct: 247 KDLLRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Sgr024654 vs. ExPASy Swiss-Prot
Match: P28583 (Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 5.3e-44
Identity = 110/278 (39.57%), Postives = 157/278 (56.47%), Query Frame = 0

Query: 5   LRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRR-------------ISPVIHL 64
           +R  Y+V  ++G+G+FGT F CT RA+GG FA K+I KR+             I  + HL
Sbjct: 30  IREVYEVGRKLGQGQFGTTFECTRRASGGKFACKSIPKRKLLCKEDYEDVWREIQIMHHL 89

Query: 65  TPNASLTRPRSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSVEVQAA 124
           + +A++ R   +   +  +  +         F            +    +S    E QAA
Sbjct: 90  SEHANVVRIEGTYEDSTAVHLVMELCEGGELFDRI---------VQKGHYS----ERQAA 149

Query: 125 SIMSQLMHAVAHCHRLGVAHRDIKPDNILFDEWD---SVKLADFGSAEMFKQGQESMSGI 184
            ++  ++  V  CH LGV HRD+KP+N LFD  D    +K  DFG +  +K G ES   +
Sbjct: 150 RLIKTIVEVVEACHSLGVMHRDLKPENFLFDTIDEDAKLKATDFGLSVFYKPG-ESFCDV 209

Query: 185 VGTPYYVAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFP 244
           VG+PYYVAPEVL  K YG + DVWSAGV+LY++L+G PPF  ES   IF+ +L   L F 
Sbjct: 210 VGSPYYVAPEVLR-KLYGPESDVWSAGVILYILLSGVPPFWAESEPGIFRQILLGKLDFH 269

Query: 245 TRVFHSVSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWI 267
           +  + S+S +AKDL+RKML ++   R +A +VLRHPWI
Sbjct: 270 SEPWPSISDSAKDLIRKMLDQNPKTRLTAHEVLRHPWI 292

BLAST of Sgr024654 vs. ExPASy Swiss-Prot
Match: Q8W490 (Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2 PE=2 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 1.7e-42
Identity = 110/267 (41.20%), Postives = 154/267 (57.68%), Query Frame = 0

Query: 8   DYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDK------RRISPVIHLTPNASLTRPR 67
           DY     IG+G+FG+V  C SR  G  FA KT+ K      R +  + HL+ +  +    
Sbjct: 106 DYVFGRNIGKGKFGSVRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVV--- 165

Query: 68  SSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLP--ISTASHSPGSVEVQAASIMSQLMH 127
                TL   +  S  F   +      S   L+   +    +S    E +AA+I   LM 
Sbjct: 166 -----TLHAVYEESDCF---HLVMELCSGGRLIDQMVKVGRYS----EQRAANIFKDLML 225

Query: 128 AVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEV 187
            + +CH +GV HRDIKP+NIL      ++LADFG A    +GQ ++SG+ G+P YVAPEV
Sbjct: 226 VINYCHEMGVVHRDIKPENILLTAAGKIQLADFGLAMRIAKGQ-TLSGLAGSPAYVAPEV 285

Query: 188 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAA 247
           L+ ++Y EKVDVWSAGV+LY +L+G  PF G+S+  IF+A+    L F T V+ SVS  A
Sbjct: 286 LS-ENYSEKVDVWSAGVLLYALLSGVLPFKGDSLDAIFEAIKNVKLDFNTGVWESVSKPA 345

Query: 248 KDLLRKMLCKDVSRRFSAVQVLRHPWI 267
           +DLL +ML ++ S R +A +VLRHPWI
Sbjct: 346 RDLLARMLTREESARITADEVLRHPWI 355

BLAST of Sgr024654 vs. ExPASy Swiss-Prot
Match: Q53P85 (Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=CPK24 PE=1 SV=1)

HSP 1 Score: 173.3 bits (438), Expect = 3.8e-42
Identity = 105/278 (37.77%), Postives = 155/278 (55.76%), Query Frame = 0

Query: 5   LRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRR-------------ISPVIHL 64
           +R  Y++ +++G+G+FGT + C  +A+GG FA K+I KR+             I  + HL
Sbjct: 43  VRDHYRIGKKLGQGQFGTTYLCVDKASGGEFACKSIPKRKLLCREDYEDVWREIQIMHHL 102

Query: 65  TPNASLTRPRSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSVEVQAA 124
           + + ++ R R +    L +  +         F            I    H     E  AA
Sbjct: 103 SEHPNVVRIRGAYEDALFVHIVMELCAGGELFDR----------IVAKGH---YTERAAA 162

Query: 125 SIMSQLMHAVAHCHRLGVAHRDIKPDNILF---DEWDSVKLADFGSAEMFKQGQESMSGI 184
            ++  ++  V  CH LGV HRD+KP+N LF    E   +K  DFG +  +K G +  S +
Sbjct: 163 QLIRTIVAVVEGCHSLGVMHRDLKPENFLFASAAEDAPLKATDFGLSMFYKPG-DKFSDV 222

Query: 185 VGTPYYVAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFP 244
           VG+PYYVAPEVL  K YG + DVWSAGV+LY++L G PPF  E+   IF+ +LR  L F 
Sbjct: 223 VGSPYYVAPEVLQ-KCYGPESDVWSAGVILYILLCGVPPFWAETEAGIFRQILRGKLDFE 282

Query: 245 TRVFHSVSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWI 267
           +  + S+S +AKDL+R MLC+D ++R +A +VL HPWI
Sbjct: 283 SEPWPSISDSAKDLVRNMLCRDPTKRLTAHEVLCHPWI 305

BLAST of Sgr024654 vs. ExPASy TrEMBL
Match: A0A6J1CYJ8 (phosphoenolpyruvate carboxylase kinase 1-like OS=Momordica charantia OX=3673 GN=LOC111015644 PE=3 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 7.3e-105
Identity = 211/284 (74.30%), Postives = 227/284 (79.93%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASL--T 60
           MSDAL+RDYQV EEIGRGRFGTVFRCTSRA+G F+AVKTID+RRIS    L     +  T
Sbjct: 1   MSDALKRDYQVIEEIGRGRFGTVFRCTSRASGEFYAVKTIDRRRISAGDSLDAECLVNET 60

Query: 61  RPRSSIFS---TLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSVEVQAASIMS 120
           +    ++S    L L  L    F         SS+     I+    S    E QAASIMS
Sbjct: 61  KILHLLYSHPHILALHDLYEDEFHLHMVLDLCSSSDLHTRITLQVFS----EAQAASIMS 120

Query: 121 QLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYYV 180
           QLM A+AHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMF+QG+ESM+G+VGTPYYV
Sbjct: 121 QLMQALAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFRQGEESMNGVVGTPYYV 180

Query: 181 APEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSV 240
           APEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESV EIF AVLRANLRFP+RVFHSV
Sbjct: 181 APEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVAEIFHAVLRANLRFPSRVFHSV 240

Query: 241 SPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATELA 280
           SPAAKDLLRKMLCKDV RRFSA QVLRHPWITSCGG RPATELA
Sbjct: 241 SPAAKDLLRKMLCKDVFRRFSAEQVLRHPWITSCGGARPATELA 280

BLAST of Sgr024654 vs. ExPASy TrEMBL
Match: A0A6J1ED04 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111432056 PE=3 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 2.1e-99
Identity = 201/284 (70.77%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MSD L+RDYQV EEIGRGRFGTVFRCTSR++G  +AVKTIDKRRI     L     +   
Sbjct: 1   MSDDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVKET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N          +L + ++S     V      E +AA IM
Sbjct: 61  K-----ILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM G+VGTPYY
Sbjct: 121 SQLMHAVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSPAAKDLLRKMLCKDVSRR SA QVLRHPWITSC  +R AT L
Sbjct: 241 VSPAAKDLLRKMLCKDVSRRISAEQVLRHPWITSCVESRAATAL 279

BLAST of Sgr024654 vs. ExPASy TrEMBL
Match: A0A6J1J089 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111480088 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.7e-98
Identity = 199/284 (70.07%), Postives = 217/284 (76.41%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MS+ L+RDYQV EEIGRGRFGTVFRCTSR++G  +AVKTIDKRRI     L     +   
Sbjct: 1   MSNDLKRDYQVIEEIGRGRFGTVFRCTSRSSGENYAVKTIDKRRILAGDSLDAECLVNET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N          +L + ++S     V      E +AA IM
Sbjct: 61  K-----ILHLLYPHPHILALHNLYEDEFHLHMVLDLCSSSDLHRRVTLQVFSEAEAAMIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM G+VGTPYY
Sbjct: 121 SQLMHAVAHCHRYGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMRGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSPAAKDLLRKMLC+DVSRR SA QVLRHPWITSC  +R AT L
Sbjct: 241 VSPAAKDLLRKMLCRDVSRRISADQVLRHPWITSCVESRAATAL 279

BLAST of Sgr024654 vs. ExPASy TrEMBL
Match: A0A5D3D228 (Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001580 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.6e-96
Identity = 194/284 (68.31%), Postives = 214/284 (75.35%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MSD  +RDYQV EE+GRGRFG VFRC SR++GG +AVK IDKRRIS    L     +   
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N     S    +L + ++S     +      E +AA IM
Sbjct: 61  K-----ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM+G+VGTPYY
Sbjct: 121 SQLMHAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSP+AKDLLRKMLCKDVSRR SA QVLRHPWIT+       TEL
Sbjct: 241 VSPSAKDLLRKMLCKDVSRRISAEQVLRHPWITNYVENMAETEL 279

BLAST of Sgr024654 vs. ExPASy TrEMBL
Match: A0A1S3AWP8 (phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC103483595 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.6e-96
Identity = 194/284 (68.31%), Postives = 214/284 (75.35%), Query Frame = 0

Query: 1   MSDALRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRP 60
           MSD  +RDYQV EE+GRGRFG VFRC SR++GG +AVK IDKRRIS    L     +   
Sbjct: 1   MSDGFKRDYQVIEELGRGRFGIVFRCISRSSGGNYAVKIIDKRRISAGDSLDAECLVNET 60

Query: 61  RSSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSV------EVQAASIM 120
           +      L L +    I    N     S    +L + ++S     +      E +AA IM
Sbjct: 61  K-----ILHLLYPHPHILALHNLYEDESHLHMVLDLCSSSDLHRRITLQIFSEAEAARIM 120

Query: 121 SQLMHAVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYY 180
           SQLMHAVAHCHR GVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQG+ESM+G+VGTPYY
Sbjct: 121 SQLMHAVAHCHRHGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGEESMTGVVGTPYY 180

Query: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHS 240
           VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIF AVLRANLRFP+RVFHS
Sbjct: 181 VAPEVLAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFHAVLRANLRFPSRVFHS 240

Query: 241 VSPAAKDLLRKMLCKDVSRRFSAVQVLRHPWITSCGGTRPATEL 279
           VSP+AKDLLRKMLCKDVSRR SA QVLRHPWIT+       TEL
Sbjct: 241 VSPSAKDLLRKMLCKDVSRRISAEQVLRHPWITNYVENMAETEL 279

BLAST of Sgr024654 vs. TAIR 10
Match: AT1G08650.1 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 247.3 bits (630), Expect = 1.5e-65
Identity = 138/266 (51.88%), Postives = 170/266 (63.91%), Query Frame = 0

Query: 9   YQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSIFSTL 68
           YQ+ EEIGRGRFGTV R  + A G FFA KTIDK  +S  +      +  +  + +    
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSYHP 74

Query: 69  TLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGS-VEVQAASIMSQLMHAVAHCHRL 128
            +  +   I T +  +         + I     S G+  E Q AS   Q++ A++HCHR 
Sbjct: 75  NIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCHRY 134

Query: 129 GVAHRDIKPDNILFD-EWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEVLAGKDYG 188
           GV HRDIKP+NIL D   D+VK+ DFGS     +G E+  G+VGTPYYVAPEVL G  YG
Sbjct: 135 GVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYSYG 194

Query: 189 EKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAAKDLLRKM 248
           EKVD+WSAGVVLY MLAG PPF+GE+  EIF+AVLR NLRFPT++F  VS  AKD LRK+
Sbjct: 195 EKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRKL 254

Query: 249 LCKDVSRRFSAVQVLRHPWITSCGGT 273
           +CKD SRRFSA Q LRHPWI   G T
Sbjct: 255 ICKDASRRFSAEQALRHPWIQRAGET 279

BLAST of Sgr024654 vs. TAIR 10
Match: AT1G08650.2 (phosphoenolpyruvate carboxylase kinase 1 )

HSP 1 Score: 230.7 bits (587), Expect = 1.4e-60
Identity = 131/255 (51.37%), Postives = 163/255 (63.92%), Query Frame = 0

Query: 9   YQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSIFSTL 68
           YQ+ EEIGRGRFGTV R  + A G FFA KTIDK  +S  +      +  +  + +    
Sbjct: 15  YQICEEIGRGRFGTVSRVYAPATGDFFACKTIDKASLSDDLDRACLDNEPKLMALLSYHP 74

Query: 69  TLTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGS-VEVQAASIMSQLMHAVAHCHRL 128
            +  +   I T +  +         + I     S G+  E Q AS   Q++ A++HCHR 
Sbjct: 75  NIVQIHDLIDTDSTLSIFMELVHPSVSIYDRLVSSGTFFEPQTASFAKQILQALSHCHRY 134

Query: 129 GVAHRDIKPDNILFD-EWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEVLAGKDYG 188
           GV HRDIKP+NIL D   D+VK+ DFGS     +G E+  G+VGTPYYVAPEVL G  YG
Sbjct: 135 GVVHRDIKPENILVDLRNDTVKICDFGSGIWLGEG-ETTEGVVGTPYYVAPEVLMGYSYG 194

Query: 189 EKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAAKDLLRKM 248
           EKVD+WSAGVVLY MLAG PPF+GE+  EIF+AVLR NLRFPT++F  VS  AKD LRK+
Sbjct: 195 EKVDLWSAGVVLYTMLAGTPPFYGETAEEIFEAVLRGNLRFPTKIFRGVSSMAKDFLRKL 254

Query: 249 LCKDVSRRFSAVQVL 262
           +CKD SRRFSA Q L
Sbjct: 255 ICKDASRRFSAEQAL 268

BLAST of Sgr024654 vs. TAIR 10
Match: AT3G04530.1 (phosphoenolpyruvate carboxylase kinase 2 )

HSP 1 Score: 228.4 bits (581), Expect = 7.1e-60
Identity = 132/271 (48.71%), Postives = 167/271 (61.62%), Query Frame = 0

Query: 5   LRRDYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSI 64
           L  +YQ+ +EIGRGRFGT+ RC S A   F+A KTIDKR +  +  L      T PR   
Sbjct: 7   LENNYQLCDEIGRGRFGTITRCFSPATKEFYACKTIDKRVL--IDALDRECIETEPRIMA 66

Query: 65  FSTLTLTFLR--STIFTKTNFTSTWSSTSALLPISTASHSPGS--VEVQAASIMSQLMHA 124
                   +R      T+ +           + I     S G    E ++AS   Q++ A
Sbjct: 67  MLPPHPNIIRIFDLYETEDSLAIVMELVDPPMTIYDRLISAGGRLSESESASYAKQILSA 126

Query: 125 VAHCHRLGVAHRDIKPDNILFD-EWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEV 184
           +AHCHR  V HRD+KPDN+L D     VKL DFGSA     G E+  G+VGTPYYVAPEV
Sbjct: 127 LAHCHRCDVVHRDVKPDNVLVDLVSGGVKLCDFGSAVWL--GGETAEGVVGTPYYVAPEV 186

Query: 185 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAA 244
           + G+ Y EKVD+WSAGVV+Y MLAG PPF+GE+  +IF+++LR NLRFP + F SVS  A
Sbjct: 187 VMGRKYDEKVDIWSAGVVIYTMLAGEPPFNGETAEDIFESILRGNLRFPPKKFGSVSSEA 246

Query: 245 KDLLRKMLCKDVSRRFSAVQVLRHPWITSCG 271
           KDLLRKM+C+DVSRRFSA   LRH W+ + G
Sbjct: 247 KDLLRKMICRDVSRRFSAEDALRHSWMMNVG 273

BLAST of Sgr024654 vs. TAIR 10
Match: AT1G12680.1 (phosphoenolpyruvate carboxylase-related kinase 2 )

HSP 1 Score: 174.5 bits (441), Expect = 1.2e-43
Identity = 110/267 (41.20%), Postives = 154/267 (57.68%), Query Frame = 0

Query: 8   DYQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDK------RRISPVIHLTPNASLTRPR 67
           DY     IG+G+FG+V  C SR  G  FA KT+ K      R +  + HL+ +  +    
Sbjct: 106 DYVFGRNIGKGKFGSVRICKSRKNGTEFACKTLKKGEETVHREVEIMQHLSGHPRVV--- 165

Query: 68  SSIFSTLTLTFLRSTIFTKTNFTSTWSSTSALLP--ISTASHSPGSVEVQAASIMSQLMH 127
                TL   +  S  F   +      S   L+   +    +S    E +AA+I   LM 
Sbjct: 166 -----TLHAVYEESDCF---HLVMELCSGGRLIDQMVKVGRYS----EQRAANIFKDLML 225

Query: 128 AVAHCHRLGVAHRDIKPDNILFDEWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEV 187
            + +CH +GV HRDIKP+NIL      ++LADFG A    +GQ ++SG+ G+P YVAPEV
Sbjct: 226 VINYCHEMGVVHRDIKPENILLTAAGKIQLADFGLAMRIAKGQ-TLSGLAGSPAYVAPEV 285

Query: 188 LAGKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAA 247
           L+ ++Y EKVDVWSAGV+LY +L+G  PF G+S+  IF+A+    L F T V+ SVS  A
Sbjct: 286 LS-ENYSEKVDVWSAGVLLYALLSGVLPFKGDSLDAIFEAIKNVKLDFNTGVWESVSKPA 345

Query: 248 KDLLRKMLCKDVSRRFSAVQVLRHPWI 267
           +DLL +ML ++ S R +A +VLRHPWI
Sbjct: 346 RDLLARMLTREESARITADEVLRHPWI 355

BLAST of Sgr024654 vs. TAIR 10
Match: AT1G76040.2 (calcium-dependent protein kinase 29 )

HSP 1 Score: 165.6 bits (418), Expect = 5.6e-41
Identity = 98/266 (36.84%), Postives = 150/266 (56.39%), Query Frame = 0

Query: 9   YQVNEEIGRGRFGTVFRCTSRAAGGFFAVKTIDKRRISPVIHLTPNASLTRPRSSIFSTL 68
           Y +++E+GRG+FG  ++CT ++ G  +A K+I KR++        +    R    I   L
Sbjct: 112 YDLHKELGRGQFGITYKCTDKSNGREYACKSISKRKLI----RRKDIEDVRREVMILQHL 171

Query: 69  T----LTFLRSTIFTKTNFTSTWSSTSALLPISTASHSPGSVEVQAASIMSQLMHAVAHC 128
           T    +   R     K N        S               E +AA+I  Q+++ V  C
Sbjct: 172 TGQPNIVEFRGAYEDKDNLHLVMELCSGGELFDRIIKKGSYSEKEAANIFRQIVNVVHVC 231

Query: 129 HRLGVAHRDIKPDNILF---DEWDSVKLADFGSAEMFKQGQESMSGIVGTPYYVAPEVLA 188
           H +GV HRD+KP+N L    +E   +K  DFG +   ++G +    IVG+ YYVAPEVL 
Sbjct: 232 HFMGVVHRDLKPENFLLVSNEEDSPIKATDFGLSVFIEEG-KVYRDIVGSAYYVAPEVL- 291

Query: 189 GKDYGEKVDVWSAGVVLYVMLAGFPPFHGESVVEIFQAVLRANLRFPTRVFHSVSPAAKD 248
            ++YG+++DVWSAGV+LY++L+G PPF GE+   IF+A+L   L   T  + ++S +AKD
Sbjct: 292 HRNYGKEIDVWSAGVMLYILLSGVPPFWGETEKTIFEAILEGKLDLETSPWPTISESAKD 351

Query: 249 LLRKMLCKDVSRRFSAVQVLRHPWIT 268
           L+RKML +D  +R +A + L HPW+T
Sbjct: 352 LIRKMLIRDPKKRITAAEALEHPWMT 371

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022146428.11.5e-10474.30phosphoenolpyruvate carboxylase kinase 1-like [Momordica charantia][more]
XP_038900016.13.5e-10170.77phosphoenolpyruvate carboxylase kinase 1-like [Benincasa hispida][more]
XP_022924623.14.2e-9970.77phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita moschata][more]
KAG6582384.11.6e-9870.42Phosphoenolpyruvate carboxylase kinase 1, partial [Cucurbita argyrosperma subsp.... [more]
XP_023526782.12.1e-9870.42phosphoenolpyruvate carboxylase kinase 1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9SPK42.1e-6451.88Phosphoenolpyruvate carboxylase kinase 1 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
Q93VK09.9e-5948.71Phosphoenolpyruvate carboxylase kinase 2 OS=Arabidopsis thaliana OX=3702 GN=PPCK... [more]
P285835.3e-4439.57Calcium-dependent protein kinase SK5 OS=Glycine max OX=3847 PE=1 SV=1[more]
Q8W4901.7e-4241.20Serine/threonine-protein kinase PEPKR2 OS=Arabidopsis thaliana OX=3702 GN=PEPKR2... [more]
Q53P853.8e-4237.77Calcium-dependent protein kinase 24 OS=Oryza sativa subsp. japonica OX=39947 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1CYJ87.3e-10574.30phosphoenolpyruvate carboxylase kinase 1-like OS=Momordica charantia OX=3673 GN=... [more]
A0A6J1ED042.1e-9970.77phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1J0891.7e-9870.07phosphoenolpyruvate carboxylase kinase 1-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5D3D2281.6e-9668.31Phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3AWP81.6e-9668.31phosphoenolpyruvate carboxylase kinase 1-like OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT1G08650.11.5e-6551.88phosphoenolpyruvate carboxylase kinase 1 [more]
AT1G08650.21.4e-6051.37phosphoenolpyruvate carboxylase kinase 1 [more]
AT3G04530.17.1e-6048.71phosphoenolpyruvate carboxylase kinase 2 [more]
AT1G12680.11.2e-4341.20phosphoenolpyruvate carboxylase-related kinase 2 [more]
AT1G76040.25.6e-4136.84calcium-dependent protein kinase 29 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 9..266
e-value: 3.4E-61
score: 219.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 9..266
e-value: 4.7E-57
score: 193.3
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 9..266
score: 39.028934
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 4..63
e-value: 7.9E-10
score: 40.8
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 97..276
e-value: 3.2E-61
score: 209.3
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 110..267
e-value: 7.2E-36
score: 121.6
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 3..272
NoneNo IPR availablePANTHERPTHR24349:SF410PHOSPHOENOLPYRUVATE CARBOXYLASE KINASE 1coord: 3..272
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 129..141
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 15..38
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 6..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr024654.1Sgr024654.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity