Sgr024634 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr024634
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.2-like
Locationtig00002486: 1416752 .. 1420461 (-)
RNA-Seq ExpressionSgr024634
SyntenySgr024634
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGCTGCAGAAGAAGAAGCTGGCCTTGACGACTACACCAAAGACGGAACCGTCGATCTCAAAGGCAATCCCGTCCGTCGCTCCAATAGAGGTCGCTGGGCCGCCTGCTTCTTCATCGTCGGTAACTCTCTCTCTCTCTCTAAAATCCGCCATACTAAACGCAGTAGGTCAGAAACTTGAGAAACCCGGCCACCGGCCGTTGAACGGAGAAATTAACTTGTTGTTTTGTGGATTTAACAGTGTACGAAGTGTTCGAGCGGACGGCGTACTACGGGATATCGACGAATCTGGTAATATATTTGACGACGAAGCTTCATCAAGGCACCGTCACCTCCGCCAACAACGTCACCAACTGGACCGGAACGGTCTGGATGACGCCGATCTTGGGGGCTTACGTCGCCGACGCTCATCTCGGCCGTTACCGGACCTTCATGATCGCCTCCGCCGTCTGCCTTGCGGTATGTACTCCTCATCCTCAACCAGCTTTTTGCTTTATTAGTTATATTTCATTTTAATTAGTTTTTTTTAATCTATATTATATTTAATAATGAATCACACTCGAATAGAGATTTTTTTTTTTTTTTTTTTGAGAAAAAACTCAAATAGAGTTCTTTATTTTATAGGTCCATCGCTTTATGAAATATTTTTTCTTTATGTTTACATGACGATTCTCATTACTATTTCAAACTAATTTTACCAAACAAAGTTTTGGAATTTTCTTTATTATTAATTTCCGTTCTAATTTATCTCCCTCATGCCTTTTATAGTATTAAAAAAGTACTTCTAATTAATTAAAAAGCATCTCTTTAGGGACTCAATAGTCCCCTTATCAGAATCCTCCTAATTGTACTTTTTTACTTACAATAACTAAATCCTCAAATTTTCATAATTGTAAATATTGGACTCTACCGTCAATGTACTCAGTTAAGAATTTTGTATATTAGACTCCCACATGTGACAGACACGTGTGTTTAAATGAATTGAGAGTTAGCTTCTGATTTTCTATAAGAAGTTTTTGAGGCTAAGGAAAGAGAGGGAGAGAAATAAAAAAGAGTCAAAGTTGGACGAGGGAGAAAGAAGAGAAAAGACAAAGTGTCCAAAGGAAGATAGTGAGAAAACAAAAGAAGTTTATTGACGCCAACTAAGGAGGTCACTGGTCAAAGATATGAAGGGATGCTTCTTTTTCTATGGTTTGCTCATTTTCTTCTCACCCCAAATAAAATAGTTTGTCTTTTAGCTCATTTTCCACCAAAAGTGGAAGCCCAACCAATATAATTTTCGTGGATGCCATTACAACAACCTATAATTCAAATGTGGGATTTTACAATTGTTCCAAAAGAACATTTATCATGTTTTGTTTTCTTAAAAAACTAGTTGAAAAGAAAGAGTCTCATTTAATTAGAACATTGCTCTAAAAGTAAGACAAGTGCCTCTTTAATCTGAGCAAACACACGATTCATTTCATAGGGAATGTCTCTTCTAACGCTAGCAGTGTCGGTGCCAAGCCTGAAACCCCCGCCATGTTCGGAGGTCAACACAGAAAATTGCAAGCAGGCCTCAAGGCTACAGCTCGCTGTGTTCTTCGGCGCACTCTACACGCTGGCTCTCGGCACCGGCGGAACCAAACCCAACATCTCCACCTTCGGAGCCGACCAGTTCGACGACTTCCATCCTGAAGAGAAGGCCCAGAAGCTCTCCTTCTTCAACTGGTGGTTGTTCAGCATCTTCTTCGGCACTCTCTTCGCCACCACCACTCTCGTTTACATACAGGAATATGTCGGCTGGAGCTTGGGATATGCCATTCCCACTGTCGGCCTCGCAATATCCATTCTCATATTTGCCGCTGGCACATCCTTGTATAGGCACAAGCTCCCCACTGGAAGTCCCTTCATCAGGATGGCTAAGGTCATTGTCGCTGCTGCTAGGAATTGGAGGCTGCATCTTCCTGACGACCCTAAACAACTCCATCAGCTTGACTTCGAAGAGCACACTAAGCAAACAACTTTCAAGATTGATTCCACTCCATCTTTGAGGTCACGCTATCTCTAAAATCAGTATTCATTTGCATTTGCAGTCGGTAGGGTGAATGATTGGATTTCTGTACTGCATCATGATCATATGCAGGTTCCTGAATAAGGCTGCCATAAAAACAGGTTCAACTCATCCATGGAAGCTGTGTTCAGTGACCCAAGTGGAGGAGACAAAACAGATGCTGAGAATGATCCCAATTCTGATATGCACTTTCATCCCAAGCACAATGTTGCCACAGGCACAGACCCTATTCATCAAGCAAGGCACCACTCTCGACCGAAGCATCGGCAGCCACTTTCAAATCCCTCCTGCAAGTTTAACTGCTTTTGTTACCATCTCCATGCTTGTCAGTCTCATCATCTACGACAGGTTTGCTCGAAAATTCATTGCCAACGTTACAAAAGTCGTTAGATTGTTATATTCATTTGGATTTCATTAATCCTATTTATATGTTCATTCTCAACACAGGTTCTTCGTCAAGATAATGCAGAGACAGACCAAGAATCCAAGGGGAATCACACTACTACAAAGAATGGGAATTGGAATGATTCTCCATATCTTGATAATGACGATCGCATCTTTGGTCGAAAGGCACAGACTTTATGTTGCTAGAGAAAATGGGTTGGTGAAAAATGGAGGACAACTCCCCCTAACCATTTTCACTCTCCTCCCTCAGTTCATGCTCGTGGGAACTGCCGATGCATTTTCAGAAGTAGCAAAGATTGAGTTCTTCTACGATCAAGCACCAGAAAGCATGAAGAGTTTGGGCACTTCGTATTCGATGACTTCGCTGGGAATTGGAAACTTCCTCAGTAGTTTTCTTCTTTCAACAGTTTCTCACATTACCGAGAAACATGGCGATGGAAATGGATGGATTCTGGACAACCTGAATGCTTCTCATCTTGACTATTACTACGCGTTTTTGGCAGTGCTTAGTGCCGTCAACTTCTTCGCCTTCTTGCTCATTTCCAAAATGTATGTGTACAAAGCTGAAGTGTCTGATTCAATAAAAGTACTCACGGATGAACTCAAGGAAAAGAAACTGAAGGCAGATGAAAAATCAGCTACAATCGTTAGGAGTAGAGCCAAGGAAGAAAGAGGCCAATTACCTCCCTTCAGTTTGCTTTAAATTCAAAAATTTATATAGAAGAATTAAGATTCTAAAGTTTTTCTTCCACTCTTGCTCCTTCAAAATTTGAGAATGTAAAGATTATATACATAGAAAAATTTTGAGAATGTAAGGTTATATATATAAGTAAATCAACGAGTATCGTTACTTGATATATAGGTATATTAGTGATTTACCATGTTCATAAGTAATTAATATCTAAGTTAATCACTGAATTATATTAATTTATAACTATCACATAAAATAAAAATACACTCATTATTATTATTTAAGTTTTATCAATTATTTACCTGTTTTAAAACATTGTTATATTTACAATTATTATTTTAGAAAATAATATATTTGCAAATATCTAAAAGAAACACATATTTACCAATATTTTTTAAAAGAGATACTGATCAAAATTTCCCCTTTTGCTATTTGTTAAAATTTTTTTGAAAAAATTTAATTTTAGGTGGATTTGGATCCAGTGGGTCATCTTTGAACAGGTAGCTCATGTAGGAGTCTGGGCCATATCAATGAGCCTGCTCAGTCTAAAGCAGTTTGA

mRNA sequence

ATGGCTGCTGCAGAAGAAGAAGCTGGCCTTGACGACTACACCAAAGACGGAACCGTCGATCTCAAAGGCAATCCCGTCCGTCGCTCCAATAGAGGTCGCTGGGCCGCCTGCTTCTTCATCGTCGTGTACGAAGTGTTCGAGCGGACGGCGTACTACGGGATATCGACGAATCTGGTAATATATTTGACGACGAAGCTTCATCAAGGCACCGTCACCTCCGCCAACAACGTCACCAACTGGACCGGAACGGTCTGGATGACGCCGATCTTGGGGGCTTACGTCGCCGACGCTCATCTCGGCCGTTACCGGACCTTCATGATCGCCTCCGCCGTCTGCCTTGCGGGAATGTCTCTTCTAACGCTAGCAGTGTCGGTGCCAAGCCTGAAACCCCCGCCATGTTCGGAGGTCAACACAGAAAATTGCAAGCAGGCCTCAAGGCTACAGCTCGCTGTGTTCTTCGGCGCACTCTACACGCTGGCTCTCGGCACCGGCGGAACCAAACCCAACATCTCCACCTTCGGAGCCGACCAGTTCGACGACTTCCATCCTGAAGAGAAGGCCCAGAAGCTCTCCTTCTTCAACTGGTGGTTGTTCAGCATCTTCTTCGGCACTCTCTTCGCCACCACCACTCTCGTTTACATACAGGAATATGTCGGCTGGAGCTTGGGATATGCCATTCCCACTGTCGGCCTCGCAATATCCATTCTCATATTTGCCGCTGGCACATCCTTGTATAGGCACAAGCTCCCCACTGGAAGTCCCTTCATCAGGATGGCTAAGGTCATTGTCGCTGCTGCTAGGAATTGGAGGCTGCATCTTCCTGACGACCCTAAACAACTCCATCAGCTTGACTTCGAAGAGCACACTAAGCAAACAACTTTCAAGATTGATTCCACTCCATCTTTGAGGTTCCTGAATAAGGCTGCCATAAAAACAGGTTCAACTCATCCATGGAAGCTGTGTTCAGTGACCCAAGTGGAGGAGACAAAACAGATGCTGAGAATGATCCCAATTCTGATATGCACTTTCATCCCAAGCACAATGTTGCCACAGGCACAGACCCTATTCATCAAGCAAGGCACCACTCTCGACCGAAGCATCGGCAGCCACTTTCAAATCCCTCCTGCAAGTTTAACTGCTTTTGTTACCATCTCCATGCTTGTCAGTCTCATCATCTACGACAGGTTCTTCGTCAAGATAATGCAGAGACAGACCAAGAATCCAAGGGGAATCACACTACTACAAAGAATGGGAATTGGAATGATTCTCCATATCTTGATAATGACGATCGCATCTTTGGTCGAAAGGCACAGACTTTATGTTGCTAGAGAAAATGGGTTGGTGAAAAATGGAGGACAACTCCCCCTAACCATTTTCACTCTCCTCCCTCAGTTCATGCTCGTGGGAACTGCCGATGCATTTTCAGAAGTAGCAAAGATTGAGTTCTTCTACGATCAAGCACCAGAAAGCATGAAGAGTTTGGGCACTTCGTATTCGATGACTTCGCTGGGAATTGGAAACTTCCTCAGTAGTTTTCTTCTTTCAACAGTTTCTCACATTACCGAGAAACATGGCGATGGAAATGGATGGATTCTGGACAACCTGAATGCTTCTCATCTTGACTATTACTACGCGTTTTTGGCAGTGCTTAGTGCCGTCAACTTCTTCGCCTTCTTGCTCATTTCCAAAATGTATGTGTACAAAGCTGAAGTGTCTGATTCAATAAAAGTACTCACGGATGAACTCAAGGAAAAGAAACTGAAGGCAGATGAAAAATCAGCTACAATCGTTAGGAGTAGCTCATGTAGGAGTCTGGGCCATATCAATGAGCCTGCTCAGTCTAAAGCAGTTTGA

Coding sequence (CDS)

ATGGCTGCTGCAGAAGAAGAAGCTGGCCTTGACGACTACACCAAAGACGGAACCGTCGATCTCAAAGGCAATCCCGTCCGTCGCTCCAATAGAGGTCGCTGGGCCGCCTGCTTCTTCATCGTCGTGTACGAAGTGTTCGAGCGGACGGCGTACTACGGGATATCGACGAATCTGGTAATATATTTGACGACGAAGCTTCATCAAGGCACCGTCACCTCCGCCAACAACGTCACCAACTGGACCGGAACGGTCTGGATGACGCCGATCTTGGGGGCTTACGTCGCCGACGCTCATCTCGGCCGTTACCGGACCTTCATGATCGCCTCCGCCGTCTGCCTTGCGGGAATGTCTCTTCTAACGCTAGCAGTGTCGGTGCCAAGCCTGAAACCCCCGCCATGTTCGGAGGTCAACACAGAAAATTGCAAGCAGGCCTCAAGGCTACAGCTCGCTGTGTTCTTCGGCGCACTCTACACGCTGGCTCTCGGCACCGGCGGAACCAAACCCAACATCTCCACCTTCGGAGCCGACCAGTTCGACGACTTCCATCCTGAAGAGAAGGCCCAGAAGCTCTCCTTCTTCAACTGGTGGTTGTTCAGCATCTTCTTCGGCACTCTCTTCGCCACCACCACTCTCGTTTACATACAGGAATATGTCGGCTGGAGCTTGGGATATGCCATTCCCACTGTCGGCCTCGCAATATCCATTCTCATATTTGCCGCTGGCACATCCTTGTATAGGCACAAGCTCCCCACTGGAAGTCCCTTCATCAGGATGGCTAAGGTCATTGTCGCTGCTGCTAGGAATTGGAGGCTGCATCTTCCTGACGACCCTAAACAACTCCATCAGCTTGACTTCGAAGAGCACACTAAGCAAACAACTTTCAAGATTGATTCCACTCCATCTTTGAGGTTCCTGAATAAGGCTGCCATAAAAACAGGTTCAACTCATCCATGGAAGCTGTGTTCAGTGACCCAAGTGGAGGAGACAAAACAGATGCTGAGAATGATCCCAATTCTGATATGCACTTTCATCCCAAGCACAATGTTGCCACAGGCACAGACCCTATTCATCAAGCAAGGCACCACTCTCGACCGAAGCATCGGCAGCCACTTTCAAATCCCTCCTGCAAGTTTAACTGCTTTTGTTACCATCTCCATGCTTGTCAGTCTCATCATCTACGACAGGTTCTTCGTCAAGATAATGCAGAGACAGACCAAGAATCCAAGGGGAATCACACTACTACAAAGAATGGGAATTGGAATGATTCTCCATATCTTGATAATGACGATCGCATCTTTGGTCGAAAGGCACAGACTTTATGTTGCTAGAGAAAATGGGTTGGTGAAAAATGGAGGACAACTCCCCCTAACCATTTTCACTCTCCTCCCTCAGTTCATGCTCGTGGGAACTGCCGATGCATTTTCAGAAGTAGCAAAGATTGAGTTCTTCTACGATCAAGCACCAGAAAGCATGAAGAGTTTGGGCACTTCGTATTCGATGACTTCGCTGGGAATTGGAAACTTCCTCAGTAGTTTTCTTCTTTCAACAGTTTCTCACATTACCGAGAAACATGGCGATGGAAATGGATGGATTCTGGACAACCTGAATGCTTCTCATCTTGACTATTACTACGCGTTTTTGGCAGTGCTTAGTGCCGTCAACTTCTTCGCCTTCTTGCTCATTTCCAAAATGTATGTGTACAAAGCTGAAGTGTCTGATTCAATAAAAGTACTCACGGATGAACTCAAGGAAAAGAAACTGAAGGCAGATGAAAAATCAGCTACAATCGTTAGGAGTAGCTCATGTAGGAGTCTGGGCCATATCAATGAGCCTGCTCAGTCTAAAGCAGTTTGA

Protein sequence

MAAAEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYYAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLKADEKSATIVRSSSCRSLGHINEPAQSKAV
Homology
BLAST of Sgr024634 vs. NCBI nr
Match: XP_038902958.1 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Benincasa hispida])

HSP 1 Score: 991.9 bits (2563), Expect = 2.5e-285
Identity = 505/591 (85.45%), Postives = 543/591 (91.88%), Query Frame = 0

Query: 5   EEEAGL-DDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLT 64
           EE  G+ DDY+KDG+VDLKGNPVRRS+RGRW+ACFFIVVYEVFERTAYYG+S+NL+ YLT
Sbjct: 12  EERFGVEDDYSKDGSVDLKGNPVRRSHRGRWSACFFIVVYEVFERTAYYGVSSNLITYLT 71

Query: 65  TKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAV 124
            KLHQGTVT++NNVTNWT TVW+TPI GAY+ADAHLGRYRTF++AS +CL GMSLLTLAV
Sbjct: 72  DKLHQGTVTASNNVTNWTATVWITPIFGAYIADAHLGRYRTFIVASFICLVGMSLLTLAV 131

Query: 125 SVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHP 184
           S+PSLKP PC + N E+CKQ SRLQLAVFF ALY LALGTGGTKPNIST GADQFDDFHP
Sbjct: 132 SMPSLKPHPCLDANAEHCKQTSRLQLAVFFTALYILALGTGGTKPNISTIGADQFDDFHP 191

Query: 185 EEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTS 244
           EEKAQKLSFFNWW+FSIFFGTLFATTTLVYIQE VGWSLGY IPTVGLAI+ILIFAAGT 
Sbjct: 192 EEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAILIFAAGTP 251

Query: 245 LYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLR 304
            YRHKLPTGSPFIRMAKVIVA A NWR  LP DP+QLH+L  EE+TKQ TF+IDSTPSLR
Sbjct: 252 FYRHKLPTGSPFIRMAKVIVATAWNWRQPLPYDPRQLHELQLEEYTKQGTFRIDSTPSLR 311

Query: 305 FLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 364
           FLNKAAI+TGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTML QAQTLFIKQG TL
Sbjct: 312 FLNKAAIRTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLSQAQTLFIKQGRTL 371

Query: 365 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 424
           DRS+GSHFQIPPASLTAFVTISMLVS+IIYDRFFVKIMQR T NPRGIT+LQR+GIGMIL
Sbjct: 372 DRSVGSHFQIPPASLTAFVTISMLVSVIIYDRFFVKIMQRLTNNPRGITMLQRLGIGMIL 431

Query: 425 HILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFF 484
           HILIMTIASLVERHRL+VAR+NGL KN  QLPLTIFTLLPQFMLVG ADAFSEVAKIEFF
Sbjct: 432 HILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFSEVAKIEFF 491

Query: 485 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYY 544
           YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSH+T+KH  GNGWI +NLNASHLDYY
Sbjct: 492 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHLTKKH--GNGWINNNLNASHLDYY 551

Query: 545 YAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLKADEKSA 595
           Y FLAVLS +NFF FLL+SKMYVYKAEVSDSIKVLTD+LK   L  +EKSA
Sbjct: 552 YGFLAVLSTINFFLFLLMSKMYVYKAEVSDSIKVLTDKLKASNL--NEKSA 598

BLAST of Sgr024634 vs. NCBI nr
Match: XP_022979365.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucurbita maxima])

HSP 1 Score: 981.1 bits (2535), Expect = 4.4e-282
Identity = 507/610 (83.11%), Postives = 550/610 (90.16%), Query Frame = 0

Query: 3   AAEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYL 62
           A  EE+G+D YTKDGTVDLKGNPVRRS+RG W+ACFFIVVYEVFERTAYYGI +NL+ YL
Sbjct: 16  AVAEESGVDGYTKDGTVDLKGNPVRRSHRGGWSACFFIVVYEVFERTAYYGIFSNLITYL 75

Query: 63  TTKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLA 122
           T KLHQGTV ++NNVTNWTGTVWMTPILGAY+ADAHLGRYRTF++ASA+C  GMSLLTLA
Sbjct: 76  TDKLHQGTVAASNNVTNWTGTVWMTPILGAYIADAHLGRYRTFIVASAICFLGMSLLTLA 135

Query: 123 VSVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFH 182
           VS+PSLKPPPCSE N E+CK ASRL LAVFF ALYTLALGTGGTKPNIST GADQFDDFH
Sbjct: 136 VSMPSLKPPPCSEPNIEHCKPASRLHLAVFFTALYTLALGTGGTKPNISTIGADQFDDFH 195

Query: 183 PEEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGT 242
           PEEKAQKLSFFNWW+FSIFFGTLFATTTLVYIQ+ VGWSLGY IPTVGLAI+ILIFAAGT
Sbjct: 196 PEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQDNVGWSLGYGIPTVGLAIAILIFAAGT 255

Query: 243 SLYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSL 302
            LYRHKLPTGSPFIRMAKVI A A NWR  LP+DP+QLH+L  EE+TK+  F+IDSTPSL
Sbjct: 256 PLYRHKLPTGSPFIRMAKVIFATAWNWRQPLPNDPRQLHELQLEEYTKKGAFRIDSTPSL 315

Query: 303 RFLNKAAIKTG-STHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGT 362
           RFLNKAAI+ G STHPWKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQA TLFIKQG 
Sbjct: 316 RFLNKAAIRIGSSTHPWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAHTLFIKQGR 375

Query: 363 TLDRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGM 422
           TL+RS GS+FQIPPASLTAFVTISMLVS+IIYDR FVKIMQR T NPRGITLLQRMGIGM
Sbjct: 376 TLNRSFGSNFQIPPASLTAFVTISMLVSVIIYDRLFVKIMQRLTNNPRGITLLQRMGIGM 435

Query: 423 ILHILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIE 482
           ILHILIMTIASLVERHRL VAR+NG   +  +LPLTIFTLLPQFMLVG ADAF+EVAKIE
Sbjct: 436 ILHILIMTIASLVERHRLDVARQNG---SRQELPLTIFTLLPQFMLVGVADAFTEVAKIE 495

Query: 483 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLD 542
           FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH  GNGWI+++LNASHLD
Sbjct: 496 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH--GNGWIMNDLNASHLD 555

Query: 543 YYYAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLK---ADEKSATIVR 602
           YYYAFLAVLSA+NFF FLLISK YVYKAEVSDS+KVLT++LKE KLK    +EK AT  R
Sbjct: 556 YYYAFLAVLSAINFFLFLLISKFYVYKAEVSDSLKVLTEKLKENKLKPSNPNEKWAT-TR 615

Query: 603 SSSCRSLGHI 609
           ++S     H+
Sbjct: 616 ATSDEGRAHL 619

BLAST of Sgr024634 vs. NCBI nr
Match: XP_016898953.1 (PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo])

HSP 1 Score: 980.7 bits (2534), Expect = 5.8e-282
Identity = 500/605 (82.64%), Postives = 548/605 (90.58%), Query Frame = 0

Query: 4   AEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLT 63
           AEE    DDY+KDG+VDLKGNPVRRS+RGRW+ACFFI+VYEVFERTAYYGIS+NL+ YLT
Sbjct: 12  AEESEVEDDYSKDGSVDLKGNPVRRSHRGRWSACFFIIVYEVFERTAYYGISSNLITYLT 71

Query: 64  TKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAV 123
            KLHQGT+ ++NNVTNWT TVW+TPI GAY+ADAHLGRYRTF++AS VCL GMSLLTLAV
Sbjct: 72  DKLHQGTLQASNNVTNWTATVWITPIFGAYLADAHLGRYRTFIVASFVCLIGMSLLTLAV 131

Query: 124 SVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHP 183
           S+PSLKPPPC E N ENCKQ SRLQLA+FF ALYTLALGTGGTKPNIST GADQFDDFHP
Sbjct: 132 SMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIGADQFDDFHP 191

Query: 184 EEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTS 243
            EKAQKLSFFNWW+FSIFFGTLFATTTLVYIQE VGWSLGY IPTVGLAI+ILIFAAGT 
Sbjct: 192 GEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAILIFAAGTP 251

Query: 244 LYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLR 303
            YRHKLPTGSPFIRMAKVIVA A NWR  +P+D +QL++L  EE++KQ TF+IDST SLR
Sbjct: 252 FYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTFRIDSTSSLR 311

Query: 304 FLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 363
           FLNKAAI+TGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQG TL
Sbjct: 312 FLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGRTL 371

Query: 364 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 423
           +RS+GS FQIPPASLTAFVTISML+S+IIYDRFFVKIMQ+ T NPRGITLLQRMGIGMIL
Sbjct: 372 NRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLLQRMGIGMIL 431

Query: 424 HILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFF 483
           HILIMTIASLVERHRL+VAR+NGL KN  QLPLTIFTLLPQFMLVG ADAFSEVAKIEFF
Sbjct: 432 HILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFSEVAKIEFF 491

Query: 484 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYY 543
           YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHIT+KHG+ NGWI +NLN SHLDYY
Sbjct: 492 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGN-NGWIENNLNVSHLDYY 551

Query: 544 YAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLKADEKSATIVRSSSCR 603
           YAFLAVLSA+NFF FL++SKMY YKAEVSDSIKVLTD+LK   +  +EKSA ++R  +  
Sbjct: 552 YAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNI--NEKSA-MMRGRADE 611

Query: 604 SLGHI 609
             GH+
Sbjct: 612 GRGHL 612

BLAST of Sgr024634 vs. NCBI nr
Match: XP_023527330.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 980.3 bits (2533), Expect = 7.5e-282
Identity = 507/610 (83.11%), Postives = 549/610 (90.00%), Query Frame = 0

Query: 3   AAEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYL 62
           A  EE+G+D YTKDGTVDLKGNPVRRS+RG W+ACFFIVVYEVFERTAYYGI +NL+ YL
Sbjct: 5   AVAEESGVDGYTKDGTVDLKGNPVRRSHRGGWSACFFIVVYEVFERTAYYGIFSNLITYL 64

Query: 63  TTKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLA 122
           T KLHQGTV ++NNVTNWTGTVWMTPILGAY+ADAHLGRYRTF++ASA+C  GMSLLTLA
Sbjct: 65  TDKLHQGTVDASNNVTNWTGTVWMTPILGAYIADAHLGRYRTFIVASAICFLGMSLLTLA 124

Query: 123 VSVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFH 182
           VS+PSLKPPPC E N E+CK AS+L LAVFF ALYTLALGTGGTKPNIST GADQFDDFH
Sbjct: 125 VSMPSLKPPPCLEPNIEHCKPASKLHLAVFFTALYTLALGTGGTKPNISTIGADQFDDFH 184

Query: 183 PEEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGT 242
           PEEKAQKLSFFNWW+FSIFFGTLFATTTLVYIQ+ VGWSLGY IPTVGLAI+ILIFAAGT
Sbjct: 185 PEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQDNVGWSLGYGIPTVGLAIAILIFAAGT 244

Query: 243 SLYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSL 302
            LYRHKLPTGSPF RMAKVIVA A NWR  LP+DP+QLH+L  EE+TK+  F+IDSTPSL
Sbjct: 245 PLYRHKLPTGSPFSRMAKVIVATAWNWRQPLPNDPRQLHELQLEEYTKKGAFRIDSTPSL 304

Query: 303 RFLNKAAIKTG-STHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGT 362
           RFLNKAAI+TG STHPWKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQA TLFIKQG 
Sbjct: 305 RFLNKAAIRTGSSTHPWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAHTLFIKQGR 364

Query: 363 TLDRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGM 422
           TL+RS GS+FQIPPASLTAFVTISMLVS+IIYDR FVKIMQR T NPRGITLLQRMGIGM
Sbjct: 365 TLNRSFGSNFQIPPASLTAFVTISMLVSVIIYDRLFVKIMQRLTNNPRGITLLQRMGIGM 424

Query: 423 ILHILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIE 482
           ILHILIMTIASLVERHRL VAR+NG   +  +LPLTIFTLLPQFMLVG ADAF+EVAKIE
Sbjct: 425 ILHILIMTIASLVERHRLDVARQNG---SRQELPLTIFTLLPQFMLVGVADAFTEVAKIE 484

Query: 483 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLD 542
           FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH  GNGWI+++LNASHLD
Sbjct: 485 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH--GNGWIMNDLNASHLD 544

Query: 543 YYYAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLK---ADEKSATIVR 602
           YYYAFLAVLSA+NFF FLLISK YVYKAEVSDS+KVLT +LKE KLK    DEK AT  R
Sbjct: 545 YYYAFLAVLSAINFFLFLLISKFYVYKAEVSDSLKVLTQKLKENKLKPSNPDEKWAT-TR 604

Query: 603 SSSCRSLGHI 609
           ++S     H+
Sbjct: 605 ATSDEGRAHL 608

BLAST of Sgr024634 vs. NCBI nr
Match: KAA0049225.1 (protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17333.1 protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa])

HSP 1 Score: 979.2 bits (2530), Expect = 1.7e-281
Identity = 499/605 (82.48%), Postives = 547/605 (90.41%), Query Frame = 0

Query: 4   AEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLT 63
           AEE    DDY+KDG+VDLKGNPVRRS+RGRW+ACFFI+VYEVFERTAYYGIS+NL+ YLT
Sbjct: 12  AEESEVEDDYSKDGSVDLKGNPVRRSHRGRWSACFFIIVYEVFERTAYYGISSNLITYLT 71

Query: 64  TKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAV 123
            KLHQGT+ ++NNVTNWT TVW+TPI GAY+ADAHLGRYRTF++AS VCL GMSLLTLAV
Sbjct: 72  DKLHQGTLQASNNVTNWTATVWITPIFGAYLADAHLGRYRTFIVASFVCLIGMSLLTLAV 131

Query: 124 SVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHP 183
           S+PSLKPPPC E N ENCKQ SRLQLA+FF ALYTLALGTGGTKPNIST GADQFDDFHP
Sbjct: 132 SMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIGADQFDDFHP 191

Query: 184 EEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTS 243
            EKAQKLSFFNWW+FSIFFGTLFATTTLVYIQE VGWSLGY IPTVGLAI+ILIFAAGT 
Sbjct: 192 GEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAILIFAAGTP 251

Query: 244 LYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLR 303
            YRHKLPTGSPFIRMAKVIVA A NWR  +P+D +QL++L  EE++KQ TF+IDST SLR
Sbjct: 252 FYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTFRIDSTSSLR 311

Query: 304 FLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 363
           FLNKAAI+TGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQG TL
Sbjct: 312 FLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGRTL 371

Query: 364 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 423
           +RS+GS FQIPPASLTAFVTISML+S+IIYDRFFVKIMQ+ T NPRGITLLQRMGIGMIL
Sbjct: 372 NRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLLQRMGIGMIL 431

Query: 424 HILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFF 483
           HILIMTIASLVERHRL+VAR+NGL KN  QLPLTIFTL PQFMLVG ADAFSEVAKIEFF
Sbjct: 432 HILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLFPQFMLVGVADAFSEVAKIEFF 491

Query: 484 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYY 543
           YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHIT+KHG+ NGWI +NLN SHLDYY
Sbjct: 492 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGN-NGWIENNLNVSHLDYY 551

Query: 544 YAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLKADEKSATIVRSSSCR 603
           YAFLAVLSA+NFF FL++SKMY YKAEVSDSIKVLTD+LK   +  +EKSA ++R  +  
Sbjct: 552 YAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNI--NEKSA-MMRGRADE 611

Query: 604 SLGHI 609
             GH+
Sbjct: 612 GRGHL 612

BLAST of Sgr024634 vs. ExPASy Swiss-Prot
Match: Q9FNL7 (Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1)

HSP 1 Score: 785.4 bits (2027), Expect = 4.7e-226
Identity = 395/580 (68.10%), Postives = 471/580 (81.21%), Query Frame = 0

Query: 6   EEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTK 65
           EE G DDYTKDGTVDL+GNPVRRS RGRW AC F+VVYEVFER AYYGIS+NL IY+TTK
Sbjct: 4   EEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTK 63

Query: 66  LHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSV 125
           LHQGTV S+NNVTNW GT W+TPILGAYV DA LGRY TF+I+ A+  +GM +LTL+V++
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTI 123

Query: 126 PSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEE 185
           P +KPP CS  N ENC++AS LQLAVFFGALYTLA+GTGGTKPNIST GADQFD F P+E
Sbjct: 124 PGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPKE 183

Query: 186 KAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLY 245
           K QKLSFFNWW+FSIFFGTLFA T LVY+Q+ VGW+LGY +PT+GLAISI IF  GT  Y
Sbjct: 184 KTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPFY 243

Query: 246 RHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFL 305
           RHKLPTGSPF +MA+VIVA+ R     +  D    H+L   E+ ++  F I  TPSLRFL
Sbjct: 244 RHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRFL 303

Query: 306 NKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDR 365
           ++A++KTG+ H W LC+ T+VEETKQMLRM+P+L  TF+PS ML Q  TLF+KQGTTLDR
Sbjct: 304 DRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLDR 363

Query: 366 SIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHI 425
            +   F IPPASL+ FVT+SML+S+++YDR FVKI ++ T NPRGITLLQRMGIG+I HI
Sbjct: 364 KVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFHI 423

Query: 426 LIMTIASLVERHRLYVARENGLV-KNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFY 485
           LIM +AS+ ER+RL VA ++GL+ + G +LPLTIF LLPQF+L+G AD+F EVAK+EFFY
Sbjct: 424 LIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEFFY 483

Query: 486 DQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYY 545
           DQAPESMKSLGTSYS TSL IGNF+SSFLLSTVS IT+K   G GWIL+NLN S LDYYY
Sbjct: 484 DQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKR--GRGWILNNLNESRLDYYY 543

Query: 546 AFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKE 585
            F AVL+ VNF  FL++ K YVY+AEV+DS+ V   E+KE
Sbjct: 544 LFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Sgr024634 vs. ExPASy Swiss-Prot
Match: Q9FNL8 (Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1)

HSP 1 Score: 760.4 bits (1962), Expect = 1.6e-218
Identity = 382/573 (66.67%), Postives = 461/573 (80.45%), Query Frame = 0

Query: 6   EEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTK 65
           EE G DDYTKDGTVDL+GN VRRS  GRW AC F+VVYEVFER AYYGIS+NLVIY+TTK
Sbjct: 4   EEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTK 63

Query: 66  LHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSV 125
           LHQGTV S+NNVTNW GT W+TPILGAYVADAH GRY TF+I+SA+ L GM+LLTL+VS+
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSL 123

Query: 126 PSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEE 185
           P LKPP CS  N ENC++AS +QLAVFFGALYTLA+GTGGTKPNIST GADQFD+F P++
Sbjct: 124 PGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPKD 183

Query: 186 KAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLY 245
           K  K SFFNWW+FSIFFGT FATT LVY+Q+ VGW++GY + T+GLA SI IF  GT LY
Sbjct: 184 KIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRLY 243

Query: 246 RHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFL 305
           RHKLP GSPF +MA+VIVA+ R  R  +  D  + ++L   E+  +  F I ST SLRFL
Sbjct: 244 RHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRFL 303

Query: 306 NKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDR 365
           N+A++KTGSTH W+LC++T+VEETKQML+M+P+L  TF+PS ML Q  TLFIKQGTTLDR
Sbjct: 304 NRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLDR 363

Query: 366 SIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHI 425
            + ++F IPPASL  F T SMLVS++IYDR FVK M++ T NPRGITLLQRMGIGMILHI
Sbjct: 364 RLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILHI 423

Query: 426 LIMTIASLVERHRLYVARENGLV-KNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFY 485
           LIM IAS+ ER+RL VA E+GL  +    +PL+IFTLLPQ++L+G ADAF E+AK+EFFY
Sbjct: 424 LIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEFFY 483

Query: 486 DQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYY 545
           DQAPESMKSLGTSY+ TS+ +G F+SS LLS+VS IT+K   G GWI +NLN S LD YY
Sbjct: 484 DQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKK--QGRGWIQNNLNESRLDNYY 543

Query: 546 AFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKV 578
            F AVL+ +NF  FL++ + Y Y+A+V+ S  V
Sbjct: 544 MFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Sgr024634 vs. ExPASy Swiss-Prot
Match: Q8VZR7 (Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2)

HSP 1 Score: 568.9 bits (1465), Expect = 6.9e-161
Identity = 295/561 (52.58%), Postives = 398/561 (70.94%), Query Frame = 0

Query: 13  YTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGTVT 72
           YT+DGTVDL+G PV  S  GRW AC F++ YE FER A+YGI++NLV YLT +LH+ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 73  SANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKPPP 132
           S  NV NW+G VW+TPI GAY+AD+++GR+ TF  +S + + GM LLT+AV+V SL+P  
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRP-- 126

Query: 133 CSEVNTEN--CKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKL 192
                 EN  C +AS LQ+  F+ +LYT+A+G GGTKPNISTFGADQFD +  EEK QK+
Sbjct: 127 ----TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKV 186

Query: 193 SFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHK-L 252
           SFFNWW+FS F G LFAT  LVYIQE +GW LGY IPTVGL +S+++F  GT  YRHK +
Sbjct: 187 SFFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVI 246

Query: 253 PTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAA 312
            T +    + +V +AA +N +L  PDD  +L++LD   +      ++  TP  RFL+KAA
Sbjct: 247 KTDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAA 306

Query: 313 IKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDRSIGS 372
           IKT S  P   C+VT+VE  K++L +I I + T IPST+  Q  TLF+KQGTTLDR IGS
Sbjct: 307 IKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGS 366

Query: 373 HFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHILIMT 432
           +FQIP ASL +FVT+SML+S+ +YD+ FV  M+++T NPRGITLLQR+G+G  + I+ + 
Sbjct: 367 NFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIA 426

Query: 433 IASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFYDQAPE 492
           IAS VE  R+ V +E  +      +P++IF LLPQ+ L+G  D F+ +  +EFFYDQ+PE
Sbjct: 427 IASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSPE 486

Query: 493 SMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYYAFLAV 552
            M+SLGT++  + +G+GNFL+SFL++ +  IT K G G  WI +NLN S LDYYY FL V
Sbjct: 487 EMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSK-GGGKSWIGNNLNDSRLDYYYGFLVV 546

Query: 553 LSAVNFFAFLLISKMYVYKAE 571
           +S VN   F+  +  YVYK++
Sbjct: 547 ISIVNMGLFVWAASKYVYKSD 557

BLAST of Sgr024634 vs. ExPASy Swiss-Prot
Match: Q9M390 (Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1)

HSP 1 Score: 491.9 bits (1265), Expect = 1.1e-137
Identity = 263/569 (46.22%), Postives = 377/569 (66.26%), Query Frame = 0

Query: 11  DDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGT 70
           D YT+DGTVD+  NP  +   G W AC FI+  E  ER AYYG+ TNLV YL ++L+QG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 71  VTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKP 130
            T+ANNVTNW+GT ++TP++GA++ADA+LGRY T      + ++GM+LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 131 PPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKL 190
             C   N + C   S  Q AVFF ALY +ALGTGG KP +S+FGADQFD+    EK +K 
Sbjct: 125 GNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 191 SFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHKLP 250
           SFFNW+ FSI  G L A T LV+IQ  VGW  G+ +PTV + I++  F  G+  YR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 251 TGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAA- 310
            GSP  R+ +VIVAA R   + +P+D   L +   +E   + + K+  T +L+F +KAA 
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 311 ------IKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 370
                 IK G  +PW+LCSVTQVEE K ++ ++P+     + +T+  Q  T+F+ QG T+
Sbjct: 305 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 364

Query: 371 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 430
           D+ +G +F+IP ASL+ F T+S+L    +YD+F + + ++ T+N RG T LQRMGIG+++
Sbjct: 365 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 424

Query: 431 HILIMTIASLVERHRL-YVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEF 490
            I  M  A ++E  RL YV   N   +   Q+ ++IF  +PQ++L+G A+ F+ + ++EF
Sbjct: 425 SIFAMITAGVLEVVRLDYVKTHNAYDQK--QIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 484

Query: 491 FYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDY 550
           FYDQAP++M+SL ++ S+T++ +GN+LS+ L++ V  IT+K+G   GWI DNLN  HLDY
Sbjct: 485 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGK-PGWIPDNLNRGHLDY 544

Query: 551 YYAFLAVLSAVNFFAFLLISKMYVYKAEV 572
           ++  LA LS +NF  +L ISK Y YK  V
Sbjct: 545 FFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Sgr024634 vs. ExPASy Swiss-Prot
Match: P46032 (Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 4.3e-131
Identity = 248/563 (44.05%), Postives = 369/563 (65.54%), Query Frame = 0

Query: 13  YTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGTVT 72
           Y +DG+VD  GNP  +   G W AC FI+  E  ER AYYGI+ NL+ YLTTKLHQG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 73  SANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKPPP 132
           +A NVT W GT ++TP++GA +ADA+ GRY T    S +   GMS LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 133 CSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKLSF 192
           C     + C  A+  Q A+FFG LY +ALGTGG KP +S+FGADQFDD    E+ +K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 193 FNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHKLPTG 252
           FNW+ FSI  G L +++ LV+IQE  GW LG+ IPTV + ++I  F  GT LYR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 253 SPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAAI-- 312
           SP  R+++V+VA+ R   + +P+D   L++   +      + KI+ T   ++L+KAA+  
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 313 ----KTGS-THPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDR 372
               K+G  ++ W+LC+VTQVEE K ++RM PI     I S +  Q  T+F++QG  ++ 
Sbjct: 324 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 383

Query: 373 SIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHI 432
            IGS FQ+PPA+L  F T S+++ + +YDRF V + ++ T   +G T +QRMGIG+ + +
Sbjct: 384 KIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSV 443

Query: 433 LIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFYD 492
           L M  A++VE  RL++A + GLV++G  +P+++   +PQ+ ++G A+ F  + ++EFFYD
Sbjct: 444 LCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYD 503

Query: 493 QAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYYA 552
           Q+P++M+SL ++ ++ +  +GN+LSS +L+ V++ T ++G   GWI DNLN+ HLDY++ 
Sbjct: 504 QSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQ-EGWISDNLNSGHLDYFFW 563

Query: 553 FLAVLSAVNFFAFLLISKMYVYK 569
            LA LS VN   +   +  Y  K
Sbjct: 564 LLAGLSLVNMAVYFFSAARYKQK 581

BLAST of Sgr024634 vs. ExPASy TrEMBL
Match: A0A6J1IT17 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita maxima OX=3661 GN=LOC111479111 PE=3 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 2.1e-282
Identity = 507/610 (83.11%), Postives = 550/610 (90.16%), Query Frame = 0

Query: 3   AAEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYL 62
           A  EE+G+D YTKDGTVDLKGNPVRRS+RG W+ACFFIVVYEVFERTAYYGI +NL+ YL
Sbjct: 16  AVAEESGVDGYTKDGTVDLKGNPVRRSHRGGWSACFFIVVYEVFERTAYYGIFSNLITYL 75

Query: 63  TTKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLA 122
           T KLHQGTV ++NNVTNWTGTVWMTPILGAY+ADAHLGRYRTF++ASA+C  GMSLLTLA
Sbjct: 76  TDKLHQGTVAASNNVTNWTGTVWMTPILGAYIADAHLGRYRTFIVASAICFLGMSLLTLA 135

Query: 123 VSVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFH 182
           VS+PSLKPPPCSE N E+CK ASRL LAVFF ALYTLALGTGGTKPNIST GADQFDDFH
Sbjct: 136 VSMPSLKPPPCSEPNIEHCKPASRLHLAVFFTALYTLALGTGGTKPNISTIGADQFDDFH 195

Query: 183 PEEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGT 242
           PEEKAQKLSFFNWW+FSIFFGTLFATTTLVYIQ+ VGWSLGY IPTVGLAI+ILIFAAGT
Sbjct: 196 PEEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQDNVGWSLGYGIPTVGLAIAILIFAAGT 255

Query: 243 SLYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSL 302
            LYRHKLPTGSPFIRMAKVI A A NWR  LP+DP+QLH+L  EE+TK+  F+IDSTPSL
Sbjct: 256 PLYRHKLPTGSPFIRMAKVIFATAWNWRQPLPNDPRQLHELQLEEYTKKGAFRIDSTPSL 315

Query: 303 RFLNKAAIKTG-STHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGT 362
           RFLNKAAI+ G STHPWKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQA TLFIKQG 
Sbjct: 316 RFLNKAAIRIGSSTHPWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAHTLFIKQGR 375

Query: 363 TLDRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGM 422
           TL+RS GS+FQIPPASLTAFVTISMLVS+IIYDR FVKIMQR T NPRGITLLQRMGIGM
Sbjct: 376 TLNRSFGSNFQIPPASLTAFVTISMLVSVIIYDRLFVKIMQRLTNNPRGITLLQRMGIGM 435

Query: 423 ILHILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIE 482
           ILHILIMTIASLVERHRL VAR+NG   +  +LPLTIFTLLPQFMLVG ADAF+EVAKIE
Sbjct: 436 ILHILIMTIASLVERHRLDVARQNG---SRQELPLTIFTLLPQFMLVGVADAFTEVAKIE 495

Query: 483 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLD 542
           FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH  GNGWI+++LNASHLD
Sbjct: 496 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH--GNGWIMNDLNASHLD 555

Query: 543 YYYAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLK---ADEKSATIVR 602
           YYYAFLAVLSA+NFF FLLISK YVYKAEVSDS+KVLT++LKE KLK    +EK AT  R
Sbjct: 556 YYYAFLAVLSAINFFLFLLISKFYVYKAEVSDSLKVLTEKLKENKLKPSNPNEKWAT-TR 615

Query: 603 SSSCRSLGHI 609
           ++S     H+
Sbjct: 616 ATSDEGRAHL 619

BLAST of Sgr024634 vs. ExPASy TrEMBL
Match: A0A1S4DSL3 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 GN=LOC107990387 PE=3 SV=1)

HSP 1 Score: 980.7 bits (2534), Expect = 2.8e-282
Identity = 500/605 (82.64%), Postives = 548/605 (90.58%), Query Frame = 0

Query: 4   AEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLT 63
           AEE    DDY+KDG+VDLKGNPVRRS+RGRW+ACFFI+VYEVFERTAYYGIS+NL+ YLT
Sbjct: 12  AEESEVEDDYSKDGSVDLKGNPVRRSHRGRWSACFFIIVYEVFERTAYYGISSNLITYLT 71

Query: 64  TKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAV 123
            KLHQGT+ ++NNVTNWT TVW+TPI GAY+ADAHLGRYRTF++AS VCL GMSLLTLAV
Sbjct: 72  DKLHQGTLQASNNVTNWTATVWITPIFGAYLADAHLGRYRTFIVASFVCLIGMSLLTLAV 131

Query: 124 SVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHP 183
           S+PSLKPPPC E N ENCKQ SRLQLA+FF ALYTLALGTGGTKPNIST GADQFDDFHP
Sbjct: 132 SMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIGADQFDDFHP 191

Query: 184 EEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTS 243
            EKAQKLSFFNWW+FSIFFGTLFATTTLVYIQE VGWSLGY IPTVGLAI+ILIFAAGT 
Sbjct: 192 GEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAILIFAAGTP 251

Query: 244 LYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLR 303
            YRHKLPTGSPFIRMAKVIVA A NWR  +P+D +QL++L  EE++KQ TF+IDST SLR
Sbjct: 252 FYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTFRIDSTSSLR 311

Query: 304 FLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 363
           FLNKAAI+TGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQG TL
Sbjct: 312 FLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGRTL 371

Query: 364 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 423
           +RS+GS FQIPPASLTAFVTISML+S+IIYDRFFVKIMQ+ T NPRGITLLQRMGIGMIL
Sbjct: 372 NRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLLQRMGIGMIL 431

Query: 424 HILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFF 483
           HILIMTIASLVERHRL+VAR+NGL KN  QLPLTIFTLLPQFMLVG ADAFSEVAKIEFF
Sbjct: 432 HILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLLPQFMLVGVADAFSEVAKIEFF 491

Query: 484 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYY 543
           YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHIT+KHG+ NGWI +NLN SHLDYY
Sbjct: 492 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGN-NGWIENNLNVSHLDYY 551

Query: 544 YAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLKADEKSATIVRSSSCR 603
           YAFLAVLSA+NFF FL++SKMY YKAEVSDSIKVLTD+LK   +  +EKSA ++R  +  
Sbjct: 552 YAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNI--NEKSA-MMRGRADE 611

Query: 604 SLGHI 609
             GH+
Sbjct: 612 GRGHL 612

BLAST of Sgr024634 vs. ExPASy TrEMBL
Match: A0A5A7U6D9 (Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G001880 PE=3 SV=1)

HSP 1 Score: 979.2 bits (2530), Expect = 8.1e-282
Identity = 499/605 (82.48%), Postives = 547/605 (90.41%), Query Frame = 0

Query: 4   AEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLT 63
           AEE    DDY+KDG+VDLKGNPVRRS+RGRW+ACFFI+VYEVFERTAYYGIS+NL+ YLT
Sbjct: 12  AEESEVEDDYSKDGSVDLKGNPVRRSHRGRWSACFFIIVYEVFERTAYYGISSNLITYLT 71

Query: 64  TKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAV 123
            KLHQGT+ ++NNVTNWT TVW+TPI GAY+ADAHLGRYRTF++AS VCL GMSLLTLAV
Sbjct: 72  DKLHQGTLQASNNVTNWTATVWITPIFGAYLADAHLGRYRTFIVASFVCLIGMSLLTLAV 131

Query: 124 SVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHP 183
           S+PSLKPPPC E N ENCKQ SRLQLA+FF ALYTLALGTGGTKPNIST GADQFDDFHP
Sbjct: 132 SMPSLKPPPCLETNKENCKQTSRLQLAIFFTALYTLALGTGGTKPNISTIGADQFDDFHP 191

Query: 184 EEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTS 243
            EKAQKLSFFNWW+FSIFFGTLFATTTLVYIQE VGWSLGY IPTVGLAI+ILIFAAGT 
Sbjct: 192 GEKAQKLSFFNWWMFSIFFGTLFATTTLVYIQENVGWSLGYGIPTVGLAIAILIFAAGTP 251

Query: 244 LYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLR 303
            YRHKLPTGSPFIRMAKVIVA A NWR  +P+D +QL++L  EE++KQ TF+IDST SLR
Sbjct: 252 FYRHKLPTGSPFIRMAKVIVATALNWRQDIPNDSRQLYELQLEEYSKQGTFRIDSTSSLR 311

Query: 304 FLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 363
           FLNKAAI+TGSTH WKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQG TL
Sbjct: 312 FLNKAAIRTGSTHSWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGRTL 371

Query: 364 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 423
           +RS+GS FQIPPASLTAFVTISML+S+IIYDRFFVKIMQ+ T NPRGITLLQRMGIGMIL
Sbjct: 372 NRSVGSQFQIPPASLTAFVTISMLISVIIYDRFFVKIMQKLTNNPRGITLLQRMGIGMIL 431

Query: 424 HILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFF 483
           HILIMTIASLVERHRL+VAR+NGL KN  QLPLTIFTL PQFMLVG ADAFSEVAKIEFF
Sbjct: 432 HILIMTIASLVERHRLHVARKNGLEKNEEQLPLTIFTLFPQFMLVGVADAFSEVAKIEFF 491

Query: 484 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYY 543
           YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHIT+KHG+ NGWI +NLN SHLDYY
Sbjct: 492 YDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITKKHGN-NGWIENNLNVSHLDYY 551

Query: 544 YAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLKADEKSATIVRSSSCR 603
           YAFLAVLSA+NFF FL++SKMY YKAEVSDSIKVLTD+LK   +  +EKSA ++R  +  
Sbjct: 552 YAFLAVLSAINFFFFLVMSKMYEYKAEVSDSIKVLTDKLKASNI--NEKSA-MMRGRADE 611

Query: 604 SLGHI 609
             GH+
Sbjct: 612 GRGHL 612

BLAST of Sgr024634 vs. ExPASy TrEMBL
Match: A0A6J1ECE4 (protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431933 PE=3 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 1.1e-281
Identity = 506/610 (82.95%), Postives = 548/610 (89.84%), Query Frame = 0

Query: 3   AAEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYL 62
           A  EE+G+D YTKDGTVDLKGNPVRRS+RG W+ACFFIVVYEVFERTAYYGI +NL+ YL
Sbjct: 5   AVAEESGVDGYTKDGTVDLKGNPVRRSHRGGWSACFFIVVYEVFERTAYYGIFSNLITYL 64

Query: 63  TTKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLA 122
           T KLHQGTV ++NNVTNWTGTVWMTPILGAY+ADAHLGRYRTF++ASA+C  GMSLLTLA
Sbjct: 65  TDKLHQGTVAASNNVTNWTGTVWMTPILGAYIADAHLGRYRTFIVASAICFLGMSLLTLA 124

Query: 123 VSVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFH 182
           VS+PSLKPPPC E N E+CK ASRL LAVFF ALYTLALGTGGTKPNIST GADQFDDFH
Sbjct: 125 VSMPSLKPPPCLEPNIEHCKPASRLHLAVFFTALYTLALGTGGTKPNISTIGADQFDDFH 184

Query: 183 PEEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGT 242
           PEEKAQKLSFFNWW+FSIFFGTL ATTTLVYIQ+ VGWSLGY IPTVGLAI+ILIFAAGT
Sbjct: 185 PEEKAQKLSFFNWWMFSIFFGTLLATTTLVYIQDNVGWSLGYGIPTVGLAIAILIFAAGT 244

Query: 243 SLYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSL 302
            +YRHKLPTGSPF RMAKVIVA A NWR  LP+DP+QLH+L  EE+TK+  F+IDSTPSL
Sbjct: 245 PMYRHKLPTGSPFSRMAKVIVATAWNWRQPLPNDPRQLHELQLEEYTKKGAFRIDSTPSL 304

Query: 303 RFLNKAAIKTG-STHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGT 362
           RFLNKAAI+TG STHPWKLC+VTQVEETKQMLRMIPILICTFIPSTMLPQA TLFIKQG 
Sbjct: 305 RFLNKAAIRTGSSTHPWKLCTVTQVEETKQMLRMIPILICTFIPSTMLPQAHTLFIKQGR 364

Query: 363 TLDRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGM 422
           TL+RS GS+FQIPPASLTAFVTISMLVS+IIYDR FVKIMQR T NPRGITLLQRMGIGM
Sbjct: 365 TLNRSFGSNFQIPPASLTAFVTISMLVSVIIYDRLFVKIMQRLTNNPRGITLLQRMGIGM 424

Query: 423 ILHILIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIE 482
           ILHILIMTIASLVERHRL VAR+NG   +  +LPLTIFTLLPQFMLVG ADAF+EVAKIE
Sbjct: 425 ILHILIMTIASLVERHRLDVARQNG---SRQELPLTIFTLLPQFMLVGVADAFTEVAKIE 484

Query: 483 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLD 542
           FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH  GNGWI+++LNASHLD
Sbjct: 485 FFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKH--GNGWIMNDLNASHLD 544

Query: 543 YYYAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLK---ADEKSATIVR 602
           YYYAFLAVLSA+NFF FLLISK YVYKAEVSDS+KVLT++LKE KLK    DEK AT  R
Sbjct: 545 YYYAFLAVLSAINFFLFLLISKFYVYKAEVSDSLKVLTEKLKENKLKPSNPDEKWAT-TR 604

Query: 603 SSSCRSLGHI 609
           + S     H+
Sbjct: 605 ARSDEGRAHL 608

BLAST of Sgr024634 vs. ExPASy TrEMBL
Match: A0A6J1IQK5 (protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111479112 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.2e-277
Identity = 491/589 (83.36%), Postives = 531/589 (90.15%), Query Frame = 0

Query: 2   AAAEEEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIY 61
           A+A EE+G+DDYTKDGTVDLKGNPV RS RGRW AC FIVVYEVFER AYYGISTNL+I+
Sbjct: 3   ASAAEESGVDDYTKDGTVDLKGNPVLRSKRGRWKACSFIVVYEVFERMAYYGISTNLIIF 62

Query: 62  LTTKLHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTL 121
           LT KLHQGTV SANNVTNW+GTVW+ PILGAY+ADAHLGRYRTF+IASA+CL GM LLTL
Sbjct: 63  LTKKLHQGTVASANNVTNWSGTVWIMPILGAYIADAHLGRYRTFLIASAICLTGMGLLTL 122

Query: 122 AVSVPSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDF 181
           AVS+PSLKPPPC +VN  NCK AS LQLAVFFGALY LALGTGGTKPNIST GADQFD+F
Sbjct: 123 AVSLPSLKPPPCLDVNKGNCKPASTLQLAVFFGALYMLALGTGGTKPNISTIGADQFDEF 182

Query: 182 HPEEKAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAG 241
           HP+EKAQKLSFFNWW+FSIFFGTLFATT LVYIQ+ VGWSLGY +PT+GLAISILIF AG
Sbjct: 183 HPKEKAQKLSFFNWWMFSIFFGTLFATTILVYIQDNVGWSLGYGLPTIGLAISILIFVAG 242

Query: 242 TSLYRHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPS 301
           T  YRHKLPTGSPF +MA VIVAA RNWRL LP+DPK+LH+L FEE+ KQ  F+IDSTPS
Sbjct: 243 TPFYRHKLPTGSPFTKMASVIVAAVRNWRLPLPNDPKELHELGFEEYAKQGAFRIDSTPS 302

Query: 302 LRFLNKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGT 361
           LRFLNKAAI+ GS+  WKLC+VTQVEETKQMLRMIP+LICTF+PSTML Q  TLFIKQGT
Sbjct: 303 LRFLNKAAIRRGSSDSWKLCTVTQVEETKQMLRMIPVLICTFMPSTMLAQTHTLFIKQGT 362

Query: 362 TLDRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGM 421
           TLDRSIGSHFQIPPASL AFVTISML+S++IYDR FVKIMQR TKNPRGITLLQRMGIGM
Sbjct: 363 TLDRSIGSHFQIPPASLAAFVTISMLLSVVIYDRLFVKIMQRITKNPRGITLLQRMGIGM 422

Query: 422 ILHILIMTIASLVERHRLYVARENGLVKNG-GQ-LPLTIFTLLPQFMLVGTADAFSEVAK 481
           ILH+LIM IAS VERHRL VAR+NG V NG GQ LPLTIFTLLPQFMLVG ADAF+EVAK
Sbjct: 423 ILHVLIMIIASRVERHRLDVARQNGQVGNGSGQALPLTIFTLLPQFMLVGVADAFTEVAK 482

Query: 482 IEFFYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASH 541
           IEFFYDQAPESMKSLGTSYSMTS+GIGNFLSSFLLSTVS IT KH  GNGWI++NLNASH
Sbjct: 483 IEFFYDQAPESMKSLGTSYSMTSIGIGNFLSSFLLSTVSSITHKH--GNGWIMNNLNASH 542

Query: 542 LDYYYAFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKEKKLK 589
           LDYYYAFLAVLSA+NFF FLLISK YVYKAEVS SIK L DELK+KKLK
Sbjct: 543 LDYYYAFLAVLSAINFFLFLLISKFYVYKAEVSGSIKALADELKDKKLK 589

BLAST of Sgr024634 vs. TAIR 10
Match: AT5G46050.1 (peptide transporter 3 )

HSP 1 Score: 785.4 bits (2027), Expect = 3.3e-227
Identity = 395/580 (68.10%), Postives = 471/580 (81.21%), Query Frame = 0

Query: 6   EEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTK 65
           EE G DDYTKDGTVDL+GNPVRRS RGRW AC F+VVYEVFER AYYGIS+NL IY+TTK
Sbjct: 4   EEVG-DDYTKDGTVDLQGNPVRRSIRGRWKACSFVVVYEVFERMAYYGISSNLFIYMTTK 63

Query: 66  LHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSV 125
           LHQGTV S+NNVTNW GT W+TPILGAYV DA LGRY TF+I+ A+  +GM +LTL+V++
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVGDALLGRYITFVISCAIYFSGMMVLTLSVTI 123

Query: 126 PSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEE 185
           P +KPP CS  N ENC++AS LQLAVFFGALYTLA+GTGGTKPNIST GADQFD F P+E
Sbjct: 124 PGIKPPECSTTNVENCEKASVLQLAVFFGALYTLAIGTGGTKPNISTIGADQFDVFDPKE 183

Query: 186 KAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLY 245
           K QKLSFFNWW+FSIFFGTLFA T LVY+Q+ VGW+LGY +PT+GLAISI IF  GT  Y
Sbjct: 184 KTQKLSFFNWWMFSIFFGTLFANTVLVYVQDNVGWTLGYGLPTLGLAISITIFLLGTPFY 243

Query: 246 RHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFL 305
           RHKLPTGSPF +MA+VIVA+ R     +  D    H+L   E+ ++  F I  TPSLRFL
Sbjct: 244 RHKLPTGSPFTKMARVIVASFRKANAPMTHDITSFHELPSLEYERKGAFPIHPTPSLRFL 303

Query: 306 NKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDR 365
           ++A++KTG+ H W LC+ T+VEETKQMLRM+P+L  TF+PS ML Q  TLF+KQGTTLDR
Sbjct: 304 DRASLKTGTNHKWNLCTTTEVEETKQMLRMLPVLFITFVPSMMLAQINTLFVKQGTTLDR 363

Query: 366 SIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHI 425
            +   F IPPASL+ FVT+SML+S+++YDR FVKI ++ T NPRGITLLQRMGIG+I HI
Sbjct: 364 KVTGSFSIPPASLSGFVTLSMLISIVLYDRVFVKITRKFTGNPRGITLLQRMGIGLIFHI 423

Query: 426 LIMTIASLVERHRLYVARENGLV-KNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFY 485
           LIM +AS+ ER+RL VA ++GL+ + G +LPLTIF LLPQF+L+G AD+F EVAK+EFFY
Sbjct: 424 LIMIVASVTERYRLKVAADHGLIHQTGVKLPLTIFALLPQFVLMGMADSFLEVAKLEFFY 483

Query: 486 DQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYY 545
           DQAPESMKSLGTSYS TSL IGNF+SSFLLSTVS IT+K   G GWIL+NLN S LDYYY
Sbjct: 484 DQAPESMKSLGTSYSTTSLAIGNFMSSFLLSTVSEITKKR--GRGWILNNLNESRLDYYY 543

Query: 546 AFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKVLTDELKE 585
            F AVL+ VNF  FL++ K YVY+AEV+DS+ V   E+KE
Sbjct: 544 LFFAVLNLVNFVLFLVVVKFYVYRAEVTDSVDVKEVEMKE 580

BLAST of Sgr024634 vs. TAIR 10
Match: AT5G46040.1 (Major facilitator superfamily protein )

HSP 1 Score: 760.4 bits (1962), Expect = 1.1e-219
Identity = 382/573 (66.67%), Postives = 461/573 (80.45%), Query Frame = 0

Query: 6   EEAGLDDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTK 65
           EE G DDYTKDGTVDL+GN VRRS  GRW AC F+VVYEVFER AYYGIS+NLVIY+TTK
Sbjct: 4   EEVG-DDYTKDGTVDLRGNRVRRSQTGRWKACSFVVVYEVFERMAYYGISSNLVIYMTTK 63

Query: 66  LHQGTVTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSV 125
           LHQGTV S+NNVTNW GT W+TPILGAYVADAH GRY TF+I+SA+ L GM+LLTL+VS+
Sbjct: 64  LHQGTVKSSNNVTNWVGTSWLTPILGAYVADAHFGRYITFVISSAIYLLGMALLTLSVSL 123

Query: 126 PSLKPPPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEE 185
           P LKPP CS  N ENC++AS +QLAVFFGALYTLA+GTGGTKPNIST GADQFD+F P++
Sbjct: 124 PGLKPPKCSTANVENCEKASVIQLAVFFGALYTLAIGTGGTKPNISTIGADQFDEFDPKD 183

Query: 186 KAQKLSFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLY 245
           K  K SFFNWW+FSIFFGT FATT LVY+Q+ VGW++GY + T+GLA SI IF  GT LY
Sbjct: 184 KIHKHSFFNWWMFSIFFGTFFATTVLVYVQDNVGWAIGYGLSTLGLAFSIFIFLLGTRLY 243

Query: 246 RHKLPTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFL 305
           RHKLP GSPF +MA+VIVA+ R  R  +  D  + ++L   E+  +  F I ST SLRFL
Sbjct: 244 RHKLPMGSPFTKMARVIVASLRKAREPMSSDSTRFYELPPMEYASKRAFPIHSTSSLRFL 303

Query: 306 NKAAIKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDR 365
           N+A++KTGSTH W+LC++T+VEETKQML+M+P+L  TF+PS ML Q  TLFIKQGTTLDR
Sbjct: 304 NRASLKTGSTHKWRLCTITEVEETKQMLKMLPVLFVTFVPSMMLAQIMTLFIKQGTTLDR 363

Query: 366 SIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHI 425
            + ++F IPPASL  F T SMLVS++IYDR FVK M++ T NPRGITLLQRMGIGMILHI
Sbjct: 364 RLTNNFSIPPASLLGFTTFSMLVSIVIYDRVFVKFMRKLTGNPRGITLLQRMGIGMILHI 423

Query: 426 LIMTIASLVERHRLYVARENGLV-KNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFY 485
           LIM IAS+ ER+RL VA E+GL  +    +PL+IFTLLPQ++L+G ADAF E+AK+EFFY
Sbjct: 424 LIMIIASITERYRLKVAAEHGLTHQTAVPIPLSIFTLLPQYVLMGLADAFIEIAKLEFFY 483

Query: 486 DQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYY 545
           DQAPESMKSLGTSY+ TS+ +G F+SS LLS+VS IT+K   G GWI +NLN S LD YY
Sbjct: 484 DQAPESMKSLGTSYTSTSMAVGYFMSSILLSSVSQITKK--QGRGWIQNNLNESRLDNYY 543

Query: 546 AFLAVLSAVNFFAFLLISKMYVYKAEVSDSIKV 578
            F AVL+ +NF  FL++ + Y Y+A+V+ S  V
Sbjct: 544 MFFAVLNLLNFILFLVVIRFYEYRADVTQSANV 573

BLAST of Sgr024634 vs. TAIR 10
Match: AT2G40460.1 (Major facilitator superfamily protein )

HSP 1 Score: 568.9 bits (1465), Expect = 4.9e-162
Identity = 295/561 (52.58%), Postives = 398/561 (70.94%), Query Frame = 0

Query: 13  YTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGTVT 72
           YT+DGTVDL+G PV  S  GRW AC F++ YE FER A+YGI++NLV YLT +LH+ T++
Sbjct: 7   YTQDGTVDLQGRPVLASKTGRWRACSFLLGYEAFERMAFYGIASNLVNYLTKRLHEDTIS 66

Query: 73  SANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKPPP 132
           S  NV NW+G VW+TPI GAY+AD+++GR+ TF  +S + + GM LLT+AV+V SL+P  
Sbjct: 67  SVRNVNNWSGAVWITPIAGAYIADSYIGRFWTFTASSLIYVLGMILLTMAVTVKSLRP-- 126

Query: 133 CSEVNTEN--CKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKL 192
                 EN  C +AS LQ+  F+ +LYT+A+G GGTKPNISTFGADQFD +  EEK QK+
Sbjct: 127 ----TCENGVCNKASSLQVTFFYISLYTIAIGAGGTKPNISTFGADQFDSYSIEEKKQKV 186

Query: 193 SFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHK-L 252
           SFFNWW+FS F G LFAT  LVYIQE +GW LGY IPTVGL +S+++F  GT  YRHK +
Sbjct: 187 SFFNWWMFSSFLGALFATLGLVYIQENLGWGLGYGIPTVGLLVSLVVFYIGTPFYRHKVI 246

Query: 253 PTGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAA 312
            T +    + +V +AA +N +L  PDD  +L++LD   +      ++  TP  RFL+KAA
Sbjct: 247 KTDNLAKDLVQVPIAAFKNRKLQCPDDHLELYELDSHYYKSNGKHQVHHTPVFRFLDKAA 306

Query: 313 IKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDRSIGS 372
           IKT S  P   C+VT+VE  K++L +I I + T IPST+  Q  TLF+KQGTTLDR IGS
Sbjct: 307 IKTSSRVP---CTVTKVEVAKRVLGLIFIWLVTLIPSTLWAQVNTLFVKQGTTLDRKIGS 366

Query: 373 HFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHILIMT 432
           +FQIP ASL +FVT+SML+S+ +YD+ FV  M+++T NPRGITLLQR+G+G  + I+ + 
Sbjct: 367 NFQIPAASLGSFVTLSMLLSVPMYDQSFVPFMRKKTGNPRGITLLQRLGVGFAIQIVAIA 426

Query: 433 IASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFYDQAPE 492
           IAS VE  R+ V +E  +      +P++IF LLPQ+ L+G  D F+ +  +EFFYDQ+PE
Sbjct: 427 IASAVEVKRMRVIKEFHITSPTQVVPMSIFWLLPQYSLLGIGDVFNAIGLLEFFYDQSPE 486

Query: 493 SMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYYAFLAV 552
            M+SLGT++  + +G+GNFL+SFL++ +  IT K G G  WI +NLN S LDYYY FL V
Sbjct: 487 EMQSLGTTFFTSGIGLGNFLNSFLVTMIDKITSK-GGGKSWIGNNLNDSRLDYYYGFLVV 546

Query: 553 LSAVNFFAFLLISKMYVYKAE 571
           +S VN   F+  +  YVYK++
Sbjct: 547 ISIVNMGLFVWAASKYVYKSD 557

BLAST of Sgr024634 vs. TAIR 10
Match: AT3G54140.1 (peptide transporter 1 )

HSP 1 Score: 491.9 bits (1265), Expect = 7.6e-139
Identity = 263/569 (46.22%), Postives = 377/569 (66.26%), Query Frame = 0

Query: 11  DDYTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGT 70
           D YT+DGTVD+  NP  +   G W AC FI+  E  ER AYYG+ TNLV YL ++L+QG 
Sbjct: 5   DVYTQDGTVDIHKNPANKEKTGNWKACRFILGNECCERLAYYGMGTNLVNYLESRLNQGN 64

Query: 71  VTSANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKP 130
            T+ANNVTNW+GT ++TP++GA++ADA+LGRY T      + ++GM+LLTL+ SVP LKP
Sbjct: 65  ATAANNVTNWSGTCYITPLIGAFIADAYLGRYWTIATFVFIYVSGMTLLTLSASVPGLKP 124

Query: 131 PPCSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKL 190
             C   N + C   S  Q AVFF ALY +ALGTGG KP +S+FGADQFD+    EK +K 
Sbjct: 125 GNC---NADTCHPNSS-QTAVFFVALYMIALGTGGIKPCVSSFGADQFDENDENEKIKKS 184

Query: 191 SFFNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHKLP 250
           SFFNW+ FSI  G L A T LV+IQ  VGW  G+ +PTV + I++  F  G+  YR + P
Sbjct: 185 SFFNWFYFSINVGALIAATVLVWIQMNVGWGWGFGVPTVAMVIAVCFFFFGSRFYRLQRP 244

Query: 251 TGSPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAA- 310
            GSP  R+ +VIVAA R   + +P+D   L +   +E   + + K+  T +L+F +KAA 
Sbjct: 245 GGSPLTRIFQVIVAAFRKISVKVPEDKSLLFETADDESNIKGSRKLVHTDNLKFFDKAAV 304

Query: 311 ------IKTGSTHPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTL 370
                 IK G  +PW+LCSVTQVEE K ++ ++P+     + +T+  Q  T+F+ QG T+
Sbjct: 305 ESQSDSIKDGEVNPWRLCSVTQVEELKSIITLLPVWATGIVFATVYSQMSTMFVLQGNTM 364

Query: 371 DRSIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMIL 430
           D+ +G +F+IP ASL+ F T+S+L    +YD+F + + ++ T+N RG T LQRMGIG+++
Sbjct: 365 DQHMGKNFEIPSASLSLFDTVSVLFWTPVYDQFIIPLARKFTRNERGFTQLQRMGIGLVV 424

Query: 431 HILIMTIASLVERHRL-YVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEF 490
            I  M  A ++E  RL YV   N   +   Q+ ++IF  +PQ++L+G A+ F+ + ++EF
Sbjct: 425 SIFAMITAGVLEVVRLDYVKTHNAYDQK--QIHMSIFWQIPQYLLIGCAEVFTFIGQLEF 484

Query: 491 FYDQAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDY 550
           FYDQAP++M+SL ++ S+T++ +GN+LS+ L++ V  IT+K+G   GWI DNLN  HLDY
Sbjct: 485 FYDQAPDAMRSLCSALSLTTVALGNYLSTVLVTVVMKITKKNGK-PGWIPDNLNRGHLDY 544

Query: 551 YYAFLAVLSAVNFFAFLLISKMYVYKAEV 572
           ++  LA LS +NF  +L ISK Y YK  V
Sbjct: 545 FFYLLATLSFLNFLVYLWISKRYKYKKAV 566

BLAST of Sgr024634 vs. TAIR 10
Match: AT2G02040.1 (peptide transporter 2 )

HSP 1 Score: 469.9 bits (1208), Expect = 3.1e-132
Identity = 248/563 (44.05%), Postives = 369/563 (65.54%), Query Frame = 0

Query: 13  YTKDGTVDLKGNPVRRSNRGRWAACFFIVVYEVFERTAYYGISTNLVIYLTTKLHQGTVT 72
           Y +DG+VD  GNP  +   G W AC FI+  E  ER AYYGI+ NL+ YLTTKLHQG V+
Sbjct: 24  YAEDGSVDFNGNPPLKEKTGNWKACPFILGNECCERLAYYGIAGNLITYLTTKLHQGNVS 83

Query: 73  SANNVTNWTGTVWMTPILGAYVADAHLGRYRTFMIASAVCLAGMSLLTLAVSVPSLKPPP 132
           +A NVT W GT ++TP++GA +ADA+ GRY T    S +   GMS LTL+ SVP+LKP  
Sbjct: 84  AATNVTTWQGTCYLTPLIGAVLADAYWGRYWTIACFSGIYFIGMSALTLSASVPALKPAE 143

Query: 133 CSEVNTENCKQASRLQLAVFFGALYTLALGTGGTKPNISTFGADQFDDFHPEEKAQKLSF 192
           C     + C  A+  Q A+FFG LY +ALGTGG KP +S+FGADQFDD    E+ +K SF
Sbjct: 144 CI---GDFCPSATPAQYAMFFGGLYLIALGTGGIKPCVSSFGADQFDDTDSRERVRKASF 203

Query: 193 FNWWLFSIFFGTLFATTTLVYIQEYVGWSLGYAIPTVGLAISILIFAAGTSLYRHKLPTG 252
           FNW+ FSI  G L +++ LV+IQE  GW LG+ IPTV + ++I  F  GT LYR + P G
Sbjct: 204 FNWFYFSINIGALVSSSLLVWIQENRGWGLGFGIPTVFMGLAIASFFFGTPLYRFQKPGG 263

Query: 253 SPFIRMAKVIVAAARNWRLHLPDDPKQLHQLDFEEHTKQTTFKIDSTPSLRFLNKAAI-- 312
           SP  R+++V+VA+ R   + +P+D   L++   +      + KI+ T   ++L+KAA+  
Sbjct: 264 SPITRISQVVVASFRKSSVKVPEDATLLYETQDKNSAIAGSRKIEHTDDCQYLDKAAVIS 323

Query: 313 ----KTGS-THPWKLCSVTQVEETKQMLRMIPILICTFIPSTMLPQAQTLFIKQGTTLDR 372
               K+G  ++ W+LC+VTQVEE K ++RM PI     I S +  Q  T+F++QG  ++ 
Sbjct: 324 EEESKSGDYSNSWRLCTVTQVEELKILIRMFPIWASGIIFSAVYAQMSTMFVQQGRAMNC 383

Query: 373 SIGSHFQIPPASLTAFVTISMLVSLIIYDRFFVKIMQRQTKNPRGITLLQRMGIGMILHI 432
            IGS FQ+PPA+L  F T S+++ + +YDRF V + ++ T   +G T +QRMGIG+ + +
Sbjct: 384 KIGS-FQLPPAALGTFDTASVIIWVPLYDRFIVPLARKFTGVDKGFTEIQRMGIGLFVSV 443

Query: 433 LIMTIASLVERHRLYVARENGLVKNGGQLPLTIFTLLPQFMLVGTADAFSEVAKIEFFYD 492
           L M  A++VE  RL++A + GLV++G  +P+++   +PQ+ ++G A+ F  + ++EFFYD
Sbjct: 444 LCMAAAAIVEIIRLHMANDLGLVESGAPVPISVLWQIPQYFILGAAEVFYFIGQLEFFYD 503

Query: 493 QAPESMKSLGTSYSMTSLGIGNFLSSFLLSTVSHITEKHGDGNGWILDNLNASHLDYYYA 552
           Q+P++M+SL ++ ++ +  +GN+LSS +L+ V++ T ++G   GWI DNLN+ HLDY++ 
Sbjct: 504 QSPDAMRSLCSALALLTNALGNYLSSLILTLVTYFTTRNGQ-EGWISDNLNSGHLDYFFW 563

Query: 553 FLAVLSAVNFFAFLLISKMYVYK 569
            LA LS VN   +   +  Y  K
Sbjct: 564 LLAGLSLVNMAVYFFSAARYKQK 581

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902958.12.5e-28585.45protein NRT1/ PTR FAMILY 5.2-like isoform X1 [Benincasa hispida][more]
XP_022979365.14.4e-28283.11protein NRT1/ PTR FAMILY 5.2-like [Cucurbita maxima][more]
XP_016898953.15.8e-28282.64PREDICTED: LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo][more]
XP_023527330.17.5e-28283.11protein NRT1/ PTR FAMILY 5.2-like [Cucurbita pepo subsp. pepo][more]
KAA0049225.11.7e-28182.48protein NRT1/ PTR FAMILY 5.2-like [Cucumis melo var. makuwa] >TYK17333.1 protein... [more]
Match NameE-valueIdentityDescription
Q9FNL74.7e-22668.10Protein NRT1/ PTR FAMILY 5.2 OS=Arabidopsis thaliana OX=3702 GN=NPF5.2 PE=2 SV=1[more]
Q9FNL81.6e-21866.67Protein NRT1/ PTR FAMILY 5.3 OS=Arabidopsis thaliana OX=3702 GN=NPF5.3 PE=2 SV=1[more]
Q8VZR76.9e-16152.58Protein NRT1/ PTR FAMILY 5.1 OS=Arabidopsis thaliana OX=3702 GN=NPF5.1 PE=2 SV=2[more]
Q9M3901.1e-13746.22Protein NRT1/ PTR FAMILY 8.1 OS=Arabidopsis thaliana OX=3702 GN=NPF8.1 PE=1 SV=1[more]
P460324.3e-13144.05Protein NRT1/ PTR FAMILY 8.3 OS=Arabidopsis thaliana OX=3702 GN=NPF8.3 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IT172.1e-28283.11protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita maxima OX=3661 GN=LOC111479111 PE... [more]
A0A1S4DSL32.8e-28282.64LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo OX=3656 G... [more]
A0A5A7U6D98.1e-28282.48Protein NRT1/ PTR FAMILY 5.2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A6J1ECE41.1e-28182.95protein NRT1/ PTR FAMILY 5.2-like OS=Cucurbita moschata OX=3662 GN=LOC111431933 ... [more]
A0A6J1IQK51.2e-27783.36protein NRT1/ PTR FAMILY 5.2-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
Match NameE-valueIdentityDescription
AT5G46050.13.3e-22768.10peptide transporter 3 [more]
AT5G46040.11.1e-21966.67Major facilitator superfamily protein [more]
AT2G40460.14.9e-16252.58Major facilitator superfamily protein [more]
AT3G54140.17.6e-13946.22peptide transporter 1 [more]
AT2G02040.13.1e-13244.05peptide transporter 2 [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 575..595
NoneNo IPR availablePANTHERPTHR11654:SF453PROTEIN NRT1/ PTR FAMILY 5.2-RELATEDcoord: 17..580
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 6..573
e-value: 3.5E-193
score: 644.6
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 22..563
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 102..526
e-value: 2.7E-101
score: 339.3
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 17..580
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 34..567
e-value: 0.0
score: 672.057

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr024634.1Sgr024634.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity