Sgr024498 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr024498
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionNon-specific serine/threonine protein kinase
Locationtig00001291: 3483197 .. 3486257 (+)
RNA-Seq ExpressionSgr024498
SyntenySgr024498
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGGGAGTTCTGAGGCTGAAGATTGTGAGATTGTAGAGAAGGATCCAACGGGACGATATATACGGGTATTTGTTTGAAATTTCGAATTTGTTTGGTTTTTCTCCTTGGATTTTGTGCAACTTTTGGACACTTGAACTTTGAACTTTGAACTTTTCTCACGCCTGCCCTCTCTTTGGGTTCCTTAAAAACTGAAATTCATGGGTATTGATTTGTATGAACCATTCCTGCGGTTGTTTTCCGACGATAAAAAAATAAGGTCATTGCAGCTGGCGAAGGGGGTTTCTGCTGCATTGTCCTAGCGACTGGAATAGAGCAATTCTTATTTAAAAAAAAATTCCATTCACTATAACGCTTCCTATTTCGACATTGTAATTTTTTTTCCTATGGATATATTTATCTGATTATATATTTTTGTTCCCTAACTGTTTTACGCGGGTCTTCGATTATTCACAATTTAAAATGCGAGTGATCTTAGTTTAGGGATTCACTACTGTTTATGGATTGAATTTGTTTGTTTTGCTTTTGAAAGGGGAGAATTGGACATAAAATTGTGTTACGTTTAATCGCCATTGACCACCAAACTTGTTATTATTGATTGGTGGTTCTATCGCTAATGGTTTAATAATCGACTACTCATGTTTTAGATGTGTTCTTCAGTTTTGCTTTATTGTTGAGATGTTTTATTCTATTTGACTTTATGATTCAATCCTCATTTTAACTTTCTACTTTTCAGTACGATGAAGTTTTGGGTAAAGGGGCGTTCAAGACAGTGTATGCTTCTGCTATCTATCAATCTCAGTCCTTTTTATTCTTTTTGCTTGTCTGTTTAGCAGTTTAAGTTTGCTGCTTTTTCGCACAAGAAAGGGTATATTATTTGACTTGAGGTCTATTTTTGTTGTCGTCAGATACAAAGCATTTGACGAAGTTGGTGGAATAGAAGTTGCTTGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCGGAACAACTGCAAAGATTATATTCTGAGGTTCATCTGCTAAAGTCACTGAAACATGAAAATATCATCAAGTTCTATAGCTACTGGGTTGATGATAAGAACAAGACTATCAACATGATAACAGAATTGTTCACTTCTGGGAGTTTGAGGCAGTATGATTCAAAGAGTCTTCTTTTTATTTTCTATTATTTGTGTATTATTTCATTTTCTGCATTCAATGTGTATTTTATTCATTATGGTATACGTTCGAATTCAGATATCGAAAGAAGCACAGGAAAGTTGACATGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGTGGTTTAACATATCTGCATGGTCACAATCCTCCAATTATTCACAGGGATTTGAAATGTGATAATATATTTGTTAATGGCAATACTGGAGAAGTTAAAATTGGAGATCTTGGATTGGCAATTGTCATGCAGCAGCCTACTGCCCGAAGTGTTATTGGTAATAATTTCATTTCTAGCTTGATTTCTTTTTGTGGGCAGAATTCTAGATTGAAAGCATAATGACTTCAACTGATATTTGACATGTGATTACTATTCAGGCACCCCAGAGTTCATGGCTCCAGAGCTTTATGATGAGGACTATAATGAACTTGTCGACATATATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCGTATAATGAGTGCAAAAATTCTGCTCAAATATTCAAGAAGGTTACTTCTGTAAGTAAATGATGATCGCTACCCAATTATGTGATTAAATGTTTCCATCAAATTGCAATCCTGAAATCATTTCTTCTTTCTTGCAGGGTATAAAGCCTGCTTCCCTTGATAAAGTGTTGGACCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACCATTTGCAAGTGAACTCTTGAAAGATCCATTCCTTGCAACTGAAAATCCCAAGGAGCTTAGTTCTGAACCTTCAAGATTATTGAATGCGCAGTTCAAATCAGTAAATCCTCCACTGCTGGGATCTCATCTTATGGACATAGACCATAACTGCAGCAAACGCTCAGGCTCAATGTGCTCATCTGCCAAAAGCAATAATGAAATTTCTCACTTTTCAACTCTGGAACTTCAGAGGTTCACTGAGAATAATGAATTGACATTGAAAGGGGACATGACCGATCATAATACAATTTCATTCCATTTGCGCATTGCAGAACAATGTGGTAACTTTTTGGTCAACTCTTCCGTAATCTTCTAGAAGGCCTTTTTTCTTTAGCGAGTGTTTTAATACTCTTCATGGTTACTCGAACATTTATTTTCATACAGGTAAATCGAAGAACATCCATTTTGCCTTTTATCTTGATTCTGATACTGCTTTGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTATCATAGCTGAATTAATCGATGAATTAATTGACAAGTTTGTTCCCAGCTGGAAACCATGTACAAATTATTGTCATGGAGAAAATACTGCACACAGTTCTTCCGAAACCCAAGAGGATAAGACCTTCATTCCTCCTTTCTACTCTGAGCTAGTACTGTCATCTCCAATTGTGGCTGCTGCTAGGAAGTCTCTACTACATTTGACCGAGGTGGAAGATCAAGAAAACCAGCAGTCGATTATTTCGTGTGTATCACTGGATTACAATCACTCAACTGCCTCAGATTACAGCATTGGAAAAGGATCAGAATGTGGTGACTTCAGCCACCCTGACTGCGACAAAGCTTTTAGCGGCGAAGCAATTGAATCAGATGCTGTTGGATCTGCTGTGACGACCATTGATTTTGCAAAACCGTCTCTAATCTCCTCCTGCTCTGGAGTGTCTAAAGATTTGAGCTTGTCTAGCTATTCCTCGTTCTCTCTGGAAGAAAGAGATCACCATGATGAACTCAAGTTAGAGATTGATGCTATTGACTTGCAGTACCACCAATGCCTCTGTGAACTCTCAAGAATGAGAGAGGAAGCAATAGAAAGTGTCAAGAAGAGGTGGATGTCAAAGAACAAACCTTCTGCTGTTTGA

mRNA sequence

ATGTCTGGGAGTTCTGAGGCTGAAGATTGTGAGATTGTAGAGAAGGATCCAACGGGACGATATATACGGTACGATGAAGTTTTGGGTAAAGGGGCGTTCAAGACAGTATACAAAGCATTTGACGAAGTTGGTGGAATAGAAGTTGCTTGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCGGAACAACTGCAAAGATTATATTCTGAGGTTCATCTGCTAAAGTCACTGAAACATGAAAATATCATCAAGTTCTATAGCTACTGGGTTGATGATAAGAACAAGACTATCAACATGATAACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAAGAAGCACAGGAAAGTTGACATGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGTGGTTTAACATATCTGCATGGTCACAATCCTCCAATTATTCACAGGGATTTGAAATGTGATAATATATTTGTTAATGGCAATACTGGAGAAGTTAAAATTGGAGATCTTGGATTGGCAATTGTCATGCAGCAGCCTACTGCCCGAAGTGTTATTGGCACCCCAGAGTTCATGGCTCCAGAGCTTTATGATGAGGACTATAATGAACTTGTCGACATATATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCGTATAATGAGTGCAAAAATTCTGCTCAAATATTCAAGAAGGTTACTTCTGGTATAAAGCCTGCTTCCCTTGATAAAGTGTTGGACCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACCATTTGCAAGTGAACTCTTGAAAGATCCATTCCTTGCAACTGAAAATCCCAAGGAGCTTAGTTCTGAACCTTCAAGATTATTGAATGCGCAGTTCAAATCAGTAAATCCTCCACTGCTGGGATCTCATCTTATGGACATAGACCATAACTGCAGCAAACGCTCAGGCTCAATGTGCTCATCTGCCAAAAGCAATAATGAAATTTCTCACTTTTCAACTCTGGAACTTCAGAGGTTCACTGAGAATAATGAATTGACATTGAAAGGGGACATGACCGATCATAATACAATTTCATTCCATTTGCGCATTGCAGAACAATGTGGTAAATCGAAGAACATCCATTTTGCCTTTTATCTTGATTCTGATACTGCTTTGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTATCATAGCTGAATTAATCGATGAATTAATTGACAAGTTTGTTCCCAGCTGGAAACCATGTACAAATTATTGTCATGGAGAAAATACTGCACACAGTTCTTCCGAAACCCAAGAGGATAAGACCTTCATTCCTCCTTTCTACTCTGAGCTAGTACTGTCATCTCCAATTGTGGCTGCTGCTAGGAAGTCTCTACTACATTTGACCGAGGTGGAAGATCAAGAAAACCAGCAGTCGATTATTTCGTGTGTATCACTGGATTACAATCACTCAACTGCCTCAGATTACAGCATTGGAAAAGGATCAGAATGTGGTGACTTCAGCCACCCTGACTGCGACAAAGCTTTTAGCGGCGAAGCAATTGAATCAGATGCTGTTGGATCTGCTGTGACGACCATTGATTTTGCAAAACCGTCTCTAATCTCCTCCTGCTCTGGAGTGTCTAAAGATTTGAGCTTGTCTAGCTATTCCTCGTTCTCTCTGGAAGAAAGAGATCACCATGATGAACTCAAGTTAGAGATTGATGCTATTGACTTGCAGTACCACCAATGCCTCTGTGAACTCTCAAGAATGAGAGAGGAAGCAATAGAAAGTGTCAAGAAGAGGTGGATGTCAAAGAACAAACCTTCTGCTGTTTGA

Coding sequence (CDS)

ATGTCTGGGAGTTCTGAGGCTGAAGATTGTGAGATTGTAGAGAAGGATCCAACGGGACGATATATACGGTACGATGAAGTTTTGGGTAAAGGGGCGTTCAAGACAGTATACAAAGCATTTGACGAAGTTGGTGGAATAGAAGTTGCTTGGAGCCAAGTGGACATTGAAGATGTTTTGCAGTCACCGGAACAACTGCAAAGATTATATTCTGAGGTTCATCTGCTAAAGTCACTGAAACATGAAAATATCATCAAGTTCTATAGCTACTGGGTTGATGATAAGAACAAGACTATCAACATGATAACAGAATTGTTCACTTCTGGGAGTTTGAGGCAATATCGAAAGAAGCACAGGAAAGTTGACATGAAGGCCTTCAAGAACTGGGCAAGGCAGATACTGCGTGGTTTAACATATCTGCATGGTCACAATCCTCCAATTATTCACAGGGATTTGAAATGTGATAATATATTTGTTAATGGCAATACTGGAGAAGTTAAAATTGGAGATCTTGGATTGGCAATTGTCATGCAGCAGCCTACTGCCCGAAGTGTTATTGGCACCCCAGAGTTCATGGCTCCAGAGCTTTATGATGAGGACTATAATGAACTTGTCGACATATATTCTTTTGGCATGTGCATGTTGGAAATTGTTACTTGTGAATACCCGTATAATGAGTGCAAAAATTCTGCTCAAATATTCAAGAAGGTTACTTCTGGTATAAAGCCTGCTTCCCTTGATAAAGTGTTGGACCCTCAAGTTAAGCAGTTCATAGAGAAGTGTCTGGTTCCAGCATCCATGAGACCATTTGCAAGTGAACTCTTGAAAGATCCATTCCTTGCAACTGAAAATCCCAAGGAGCTTAGTTCTGAACCTTCAAGATTATTGAATGCGCAGTTCAAATCAGTAAATCCTCCACTGCTGGGATCTCATCTTATGGACATAGACCATAACTGCAGCAAACGCTCAGGCTCAATGTGCTCATCTGCCAAAAGCAATAATGAAATTTCTCACTTTTCAACTCTGGAACTTCAGAGGTTCACTGAGAATAATGAATTGACATTGAAAGGGGACATGACCGATCATAATACAATTTCATTCCATTTGCGCATTGCAGAACAATGTGGTAAATCGAAGAACATCCATTTTGCCTTTTATCTTGATTCTGATACTGCTTTGGCAATTGCTTTGGAGATGGTTGAACAACTTGAACTGTCAAATGAAGATGCGACTATCATAGCTGAATTAATCGATGAATTAATTGACAAGTTTGTTCCCAGCTGGAAACCATGTACAAATTATTGTCATGGAGAAAATACTGCACACAGTTCTTCCGAAACCCAAGAGGATAAGACCTTCATTCCTCCTTTCTACTCTGAGCTAGTACTGTCATCTCCAATTGTGGCTGCTGCTAGGAAGTCTCTACTACATTTGACCGAGGTGGAAGATCAAGAAAACCAGCAGTCGATTATTTCGTGTGTATCACTGGATTACAATCACTCAACTGCCTCAGATTACAGCATTGGAAAAGGATCAGAATGTGGTGACTTCAGCCACCCTGACTGCGACAAAGCTTTTAGCGGCGAAGCAATTGAATCAGATGCTGTTGGATCTGCTGTGACGACCATTGATTTTGCAAAACCGTCTCTAATCTCCTCCTGCTCTGGAGTGTCTAAAGATTTGAGCTTGTCTAGCTATTCCTCGTTCTCTCTGGAAGAAAGAGATCACCATGATGAACTCAAGTTAGAGATTGATGCTATTGACTTGCAGTACCACCAATGCCTCTGTGAACTCTCAAGAATGAGAGAGGAAGCAATAGAAAGTGTCAAGAAGAGGTGGATGTCAAAGAACAAACCTTCTGCTGTTTGA

Protein sequence

MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFKSVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMSKNKPSAV
Homology
BLAST of Sgr024498 vs. NCBI nr
Match: XP_038874814.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.7e-302
Identity = 532/626 (84.98%), Postives = 571/626 (91.21%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MSG+SEA+D EIVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 9   MSGNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 68

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 69  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKRKTINMITELFTSGSLRQYRKKHRKV 128

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNP IIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 129 DLKAFKNWARQILRGLTYLHGHNPAIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 188

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 189 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 248

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFLATE+PKE SSE SRLLN QFK
Sbjct: 249 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATESPKEHSSELSRLLNEQFK 308

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           S NPPL+ SH MD D NCSK SGS+ SS KSNN ISHFSTLELQR TENN LTLKGDMTD
Sbjct: 309 SANPPLIDSHPMDTDPNCSKLSGSVASSVKSNNGISHFSTLELQRLTENNVLTLKGDMTD 368

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKSKNIHFAFYL+SDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 369 HNTMSFHLRIAELYGKSKNIHFAFYLNSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 428

Query: 421 DKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIV--AAARKSLLHLT 480
            KFVPSWKPC NYC  +NT +SSSET E+KTFIPPF+SELVLSSP+V  AAAR +   L 
Sbjct: 429 AKFVPSWKPCPNYCEEQNTPNSSSETPEEKTFIPPFFSELVLSSPMVAAAAARNNPRDLD 488

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S++Y +ST SDYS+GKGSECG+F HP+CDKA+       ++++ V
Sbjct: 489 KVEDQENQQSIISCASVEYIYSTVSDYSMGKGSECGEFGHPECDKAYISSGTIDLDAEGV 548

Query: 541 GSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLC 600
           GS  T IDFAKPSLISSCSG+SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCLC
Sbjct: 549 GSIPTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCLC 608

Query: 601 ELSRMREEAIESVKKRWMSKNKPSAV 622
           ELSRMREEAIES KKRWMSK K + +
Sbjct: 609 ELSRMREEAIESAKKRWMSKKKATGI 634

BLAST of Sgr024498 vs. NCBI nr
Match: XP_004143946.1 (serine/threonine-protein kinase WNK8 isoform X1 [Cucumis sativus] >KGN49991.1 hypothetical protein Csa_000022 [Cucumis sativus])

HSP 1 Score: 1045.8 bits (2703), Expect = 1.5e-301
Identity = 531/626 (84.82%), Postives = 570/626 (91.05%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MSG+SEA+D  IVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 55  MSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 114

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 115 SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKV 174

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 175 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 234

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 235 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 294

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPAS R  ASELLKDPFLA E+PK+ SSE SR LN  FK
Sbjct: 295 KPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSELSRSLNEHFK 354

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLLGSH M+ DHNC+K SGS+ SS KSNN ISHFST ELQR TENNELTLKGDMTD
Sbjct: 355 SVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGDMTD 414

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 415 HNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 474

Query: 421 DKFVPSWKPCTNYC--HGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLT 480
            KFVPSWKPC NYC    +NT H S E QEDKTFI PF+SELVLSSP+VAAAR +L  L 
Sbjct: 475 AKFVPSWKPCPNYCEEQQQNTPH-SPEAQEDKTFISPFFSELVLSSPMVAAARNNLTGLA 534

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S++Y +ST SDYSIGKGSECG+F HPDC+KA+       ++++AV
Sbjct: 535 KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 594

Query: 541 GSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLC 600
           GS  TTIDFAKPSLISSCS +SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCLC
Sbjct: 595 GSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCLC 654

Query: 601 ELSRMREEAIESVKKRWMSKNKPSAV 622
           ELSRMREEAIES KKRWMSK K + +
Sbjct: 655 ELSRMREEAIESAKKRWMSKKKATGI 679

BLAST of Sgr024498 vs. NCBI nr
Match: KAA0042657.1 (serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa] >TYK06059.1 serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 1045.4 bits (2702), Expect = 1.9e-301
Identity = 530/627 (84.53%), Postives = 571/627 (91.07%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MS +SEA+D EIVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 9   MSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 68

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 69  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKV 128

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 129 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 188

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 189 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 248

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFL  E+PKE SSE SR LN QFK
Sbjct: 249 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQFK 308

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLLGSH MD DH C+K SGS+ SS KSNN ISHFST ELQR TENNELTLKG+MTD
Sbjct: 309 SVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMTD 368

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 369 HNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 428

Query: 421 DKFVPSWKPCTNYCHGE--NTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLT 480
            KFVPSWKPC NYC  E  NT  S SE QE+K+FI PF+SELVLSSP+V AAR +L  L 
Sbjct: 429 AKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGLA 488

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S++Y +ST SDYSIGKGSECG+F HPDC+KA+       ++++AV
Sbjct: 489 KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 548

Query: 541 GSAVTT-IDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCL 600
           GS ++T IDFAKPSLISSCSG+SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCL
Sbjct: 549 GSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCL 608

Query: 601 CELSRMREEAIESVKKRWMSKNKPSAV 622
           CELSRMREEAIES KKRWMSK K + +
Sbjct: 609 CELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Sgr024498 vs. NCBI nr
Match: XP_008437436.1 (PREDICTED: serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo])

HSP 1 Score: 1044.6 bits (2700), Expect = 3.3e-301
Identity = 530/627 (84.53%), Postives = 570/627 (90.91%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MS +SEA+D EIVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 9   MSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 68

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 69  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKV 128

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 129 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 188

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 189 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 248

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFL  E+PKE SSE SR LN QFK
Sbjct: 249 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQFK 308

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLLGSH MD DH C+K SGS+ SS KSNN ISHFST ELQR TENNELTLKG+MTD
Sbjct: 309 SVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMTD 368

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 369 HNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 428

Query: 421 DKFVPSWKPCTNYCHGE--NTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLT 480
            KFVPSWKPC NYC  E  NT  S SE QE+K+FI PF+SELVLSSP+V AAR +L  L 
Sbjct: 429 AKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGLA 488

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S +Y +ST SDYSIGKGSECG+F HPDC+KA+       ++++AV
Sbjct: 489 KVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 548

Query: 541 GSAVTT-IDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCL 600
           GS ++T IDFAKPSLISSCSG+SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCL
Sbjct: 549 GSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCL 608

Query: 601 CELSRMREEAIESVKKRWMSKNKPSAV 622
           CELSRMREEAIES KKRWMSK K + +
Sbjct: 609 CELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Sgr024498 vs. NCBI nr
Match: XP_022159288.1 (probable serine/threonine-protein kinase WNK10 [Momordica charantia])

HSP 1 Score: 1040.0 bits (2688), Expect = 8.1e-300
Identity = 538/653 (82.39%), Postives = 564/653 (86.37%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MSG+SE ED E+ EKDPTGRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 4   MSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 63

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD+NKTINMITELFTSGSLRQYRKKHRKV
Sbjct: 64  SPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKV 123

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ T
Sbjct: 124 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQST 183

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG 
Sbjct: 184 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGT 243

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFL  EN KE SSEPSRLLNAQF 
Sbjct: 244 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFI 303

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFST-------------------- 360
           SVNPPLLGS+ +DI H+C K SGS  SSAKS+NEISHFST                    
Sbjct: 304 SVNPPLLGSNPVDIAHSCMKLSGSESSSAKSSNEISHFSTPELSAKSSNEISHFSTLELT 363

Query: 361 ------------LELQRFTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDS 420
                       LELQRFTENNEL LKG+MTDHNTISFHLRIAEQCGKSKNIHFAFYLDS
Sbjct: 364 AKSINEVSHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDS 423

Query: 421 DTALAIALEMVEQLELSNEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQE 480
           DTALAIALEMVEQLELSNEDAT IA+LIDELI KFVPSWKPC N C         SETQE
Sbjct: 424 DTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANIC---------SETQE 483

Query: 481 DKTFIPPFYSELVLSSPIVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIG 540
           DKTFIPPF+ ELVLSSP+VA ARKSL HL ++EDQ+NQQSIISC SLDY HST S YS G
Sbjct: 484 DKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTG 543

Query: 541 KGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSF 600
           KGSE GDF HP+ +K F  E I+SD   S  T IDFAKPSLISSCSG+SKDLSLSS+SSF
Sbjct: 544 KGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSF 603

Query: 601 SLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMSKNKPSAV 622
           S+EERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWM+K K  A+
Sbjct: 604 SVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI 647

BLAST of Sgr024498 vs. ExPASy Swiss-Prot
Match: Q944Q0 (Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=1 SV=1)

HSP 1 Score: 583.9 bits (1504), Expect = 2.1e-165
Identity = 324/613 (52.85%), Postives = 415/613 (67.70%), Query Frame = 0

Query: 5   SEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 64
           S  E+ +  EKDP+GRYIRYD+VLG+GAFKTVYKAFDEV GIEVAW+ V IEDV+Q P Q
Sbjct: 12  SSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQ 71

Query: 65  LQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVDMKA 124
           L+RLYSEVHLLK+LKHENIIK +  WVD+KNKTINMITELFTSGSLR YRKKHRKVD KA
Sbjct: 72  LERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKA 131

Query: 125 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSV 184
            KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTGEVKIGDLGLA V+QQPTARSV
Sbjct: 132 IKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARSV 191

Query: 185 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPAS 244
           IGTPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPYNEC+N AQI+KKVTS IKP S
Sbjct: 192 IGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQS 251

Query: 245 LDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFKSVNP 304
           L KV DPQV+QFIEKCL+PAS RP A EL KDPFLA +  K+ +   S   ++++  V P
Sbjct: 252 LGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSALLASSSTSSKY--VRP 311

Query: 305 PLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTDHNTI 364
           P L    MD+DHN +K       S  SN +     T+ELQR  EN E  L+G+ +D  T 
Sbjct: 312 PQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVTA 371

Query: 365 SFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELIDKFV 424
           S  LRIA+  GK + +HFAFYL+SDTA AIA EMVE+L L++++  +IA++ID+ I + +
Sbjct: 372 SMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQLL 431

Query: 425 PSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLTEVEDQE 484
                 T+  H +N+   + E  E                   AA +++   +   +++ 
Sbjct: 432 ---SDRTSSHHNQNSPRLTHEDHE-------------------AANQQT---VNSKDEEA 491

Query: 485 NQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDF 544
             QS+ S +S DY       YS   G+   +          +G   ES            
Sbjct: 492 AGQSMKSDISADYYF----PYSANDGNAAME----------AGRDAES------------ 551

Query: 545 AKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEA 604
              S + SCS       +S+  + S+ + D+ ++LK E++ I+ Q++Q   +L +++E+A
Sbjct: 552 -MSSYLDSCS------MMSTIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKLKEDA 557

Query: 605 IESVKKRWMSKNK 618
           IE+ K++W++K +
Sbjct: 612 IENAKRKWITKKQ 557

BLAST of Sgr024498 vs. ExPASy Swiss-Prot
Match: Q6EU49 (Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX=39947 GN=WNK4 PE=2 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 4.9e-159
Identity = 324/626 (51.76%), Postives = 414/626 (66.13%), Query Frame = 0

Query: 6   EAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQL 65
           + +D E VE DPT RYIRY+EVLG+GA KTVYKAFDEV GIEVAWSQV+I++V+QSP+ L
Sbjct: 9   KVDDAECVEVDPTRRYIRYNEVLGRGAMKTVYKAFDEVEGIEVAWSQVEIDEVMQSPDNL 68

Query: 66  QRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVDMKAF 125
           +RLYSEVHLLKSLKHEN++KFY+YWVDD+ KTIN+ITELFTSGSLRQYR+KH +VD+KA 
Sbjct: 69  ERLYSEVHLLKSLKHENVMKFYNYWVDDQKKTINVITELFTSGSLRQYRQKHPRVDLKAI 128

Query: 126 KNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVI 185
           KNWARQ+LRGL YLH H PPIIHRDLKCDNIFVNGN GEVKIGDLGLA VM  P A+SVI
Sbjct: 129 KNWARQVLRGLDYLHTHQPPIIHRDLKCDNIFVNGNHGEVKIGDLGLATVMLTPRAKSVI 188

Query: 186 GTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASL 245
           GTPEFMAPELYDE+Y+ELVDIYSFGMCMLE+ T EYPY+EC N+AQIFKKV+ G+KPA+L
Sbjct: 189 GTPEFMAPELYDENYDELVDIYSFGMCMLEMFTLEYPYSECTNAAQIFKKVSKGVKPAAL 248

Query: 246 DKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFKSVNPP 305
            K+ + Q KQFI+KCLVPAS R  A ELL+DPFL ++N   L    ++  ++  KSV+  
Sbjct: 249 AKITNIQAKQFIDKCLVPASERLSAKELLQDPFLCSDNSSVLVG--TKFPSSLPKSVDVS 308

Query: 306 LLGSHLMDIDHNCSKRSGSMCSSAKSNNEIS--HFSTLELQRFTENNELTLKGDMTDHNT 365
           L   H MD+D N      SMC+S    N++   H S LE  R  +N EL L G+  D N+
Sbjct: 309 LEALH-MDVDTN-----ESMCTSTCKRNDLGGPHRSVLEFTRTNKNTELKLTGEKLDDNS 368

Query: 366 ISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELIDKF 425
           +S  LRIA+ CG ++NIHF FYLDSDTA+++A EMVEQLEL++ D T IA+ ID LI   
Sbjct: 369 VSLVLRIADLCGHARNIHFLFYLDSDTAMSVAAEMVEQLELADCDVTFIADFIDLLIVNL 428

Query: 426 VPSWKPCTNYC-------------HGENTAHSSSETQEDKTFIP-PFYSELVLSSPIVAA 485
           VP  +   +               H   +    SE   D   +    +S+   +SP  + 
Sbjct: 429 VPGQQLMNDAVMSTSSESKMGESEHVITSQQHPSELTHDYVLVEGMMHSKEANASP--SD 488

Query: 486 ARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIG--KGSECGDFSHPDCDKAFSG 545
              SLL+ T +    + +     V LD +  + S+Y +   +  ECG  ++   DK    
Sbjct: 489 YIDSLLNATNLGGPNSSEGSDISVQLDGSSKSLSEYGVDEYRTLECG--AYKGTDKLGCR 548

Query: 546 EAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAID 605
             + + +   A+  +D A           S    L   +S S+ E  + D L  E+  I+
Sbjct: 549 HPLSNGSSNFAIFQMDQA-----------SHHSELVIGASVSITE--NRDVLNGELGLIE 608

Query: 606 LQYHQCLCELSRMREEAIESVKKRWM 614
            QY Q   EL+RMREEA+E  +K+W+
Sbjct: 609 AQYEQWFRELTRMREEALEGARKKWL 609

BLAST of Sgr024498 vs. ExPASy Swiss-Prot
Match: Q8RXE5 (Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 GN=WNK10 PE=2 SV=1)

HSP 1 Score: 557.8 bits (1436), Expect = 1.6e-157
Identity = 320/609 (52.55%), Postives = 385/609 (63.22%), Query Frame = 0

Query: 8   EDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQR 67
           E+ + V+KDPTGRYIRY++VLG+GAFKTVYKAFDEV GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 68  LYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVDMKAFKN 127
           LYSEVHLL SLKH+NIIK +  WVDD NK+INMITELFTSGSL  YRKKHRKVD KA  N
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 128 WARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGT 187
           WARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG+VKIGDLGLA VMQQPTARSVIGT
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 188 PEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLDK 247
           PEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSGIKP SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 248 VLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFKSVNPPLL 307
           V DPQVKQFIEKCL+PA  RP A ELLKD  LA +  K+ +   S   N  FK   PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASS--NTTFKPAMPPQC 301

Query: 308 GSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTDHNTISFH 367
               MD+++     S S+CSSAKS+ E +   T+E+QR  E+ E  L G+  D    S  
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 368 LRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELIDKFVPSW 427
           LRIA   G+++ + F F L +DTA A+  EMVE+L+LS+ + T+IAE+IDELI K     
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKL---- 421

Query: 428 KPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLTEV---EDQE 487
                                                     A +SL +   V   +D+E
Sbjct: 422 -----------------------------------------KANRSLPNANSVYQSKDEE 481

Query: 488 NQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDF 547
             +S+ S +S DY H  +S+     G  C              EA+ES            
Sbjct: 482 AGESMKSEISADYYHRVSSNEGSRLGCCC--------------EAVES------------ 521

Query: 548 AKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEA 607
              S + SCS VS   S               ++LK E++ I+ QY+Q    L RM+EEA
Sbjct: 542 LLSSFLDSCSMVSNKQS---------------EDLKTELNVIESQYNQSCQRLLRMKEEA 521

Query: 608 IESVKKRWM 614
           IE  K++WM
Sbjct: 602 IEKAKRKWM 521

BLAST of Sgr024498 vs. ExPASy Swiss-Prot
Match: Q8S8Y8 (Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN=WNK6 PE=2 SV=1)

HSP 1 Score: 518.1 bits (1333), Expect = 1.4e-145
Identity = 307/631 (48.65%), Postives = 380/631 (60.22%), Query Frame = 0

Query: 2   SGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQS 61
           S   E  D E++E DPT RYIRY EV+GKGAFKTVYKAFDEV GIEVAW+QV I+DVLQS
Sbjct: 8   SALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQS 67

Query: 62  PEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVD 121
           P  L+RLYSEV LLKSLKH NII+FY+ W+DDKNKT+N+ITELFTSGSLR YRKKHRKV+
Sbjct: 68  PNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVN 127

Query: 122 MKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTA 181
           MKA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q  A
Sbjct: 128 MKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQANA 187

Query: 182 RSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIK 241
           +SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV+SGIK
Sbjct: 188 KSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIK 247

Query: 242 PASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATEN--------------PKEL 301
           PASL +V DP+VKQFIEKCL+PAS R  A ELL DPFL                  PKE 
Sbjct: 248 PASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKEG 307

Query: 302 SSEPSRLLNAQFKSVNPPLLGSHLMDIDHNC-----SKRSGSMCSSAKSNNEISHFSTLE 361
           +     L++    +  P    S  +D D N      S  SGS C              +E
Sbjct: 308 AFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRC--------------IE 367

Query: 362 LQRFTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQL 421
           ++R    N   LKG+  D  ++S  LRI ++ G+ +NIHF FY + DTA  ++ EMVEQL
Sbjct: 368 VRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQL 427

Query: 422 ELSNEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVL 481
           EL++++ T IAELID L+   +P+WK                    D T     +S+L  
Sbjct: 428 ELTDQNVTFIAELIDILLVNMIPTWK-------------------TDVTVDHLIHSQL-- 487

Query: 482 SSPIVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIGKGSEC--GDFSHPD 541
                   + S  H  E + Q+ ++++         H T    S    S+C   D     
Sbjct: 488 -------NQNSRSHHNEAKPQKQEETVF--------HDTCELVSHSCNSDCPRSDEEDKQ 547

Query: 542 CDKAFSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELK 601
           C  A  GE                   S I      ++ +SL           +  + L+
Sbjct: 548 CVDATKGE-----------------DKSSIQEVEEATEPVSL-----------EEEERLR 560

Query: 602 LEIDAIDLQYHQCLCELSRMREEAIESVKKR 612
            E++ I+ +Y + + E++  REEAI   KK+
Sbjct: 608 QELEEIEAKYQEDMKEIATKREEAIMETKKK 560

BLAST of Sgr024498 vs. ExPASy Swiss-Prot
Match: Q8LST2 (Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN=WNK7 PE=2 SV=1)

HSP 1 Score: 492.3 bits (1266), Expect = 8.2e-138
Identity = 297/631 (47.07%), Postives = 383/631 (60.70%), Query Frame = 0

Query: 1   MSGSSEAE------DCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVD 60
           M GS +A       D E++E DPT RYIRY EV+GKGA KTV+K FDEV GIEVAW+QV 
Sbjct: 1   MEGSEDASAIVEPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVR 60

Query: 61  IEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYR 120
           I+D+LQSP+ L+RLYSEV LLKSLKH+NII+FY+ W+DDKNKT+N+ITELFTSGSLRQYR
Sbjct: 61  IDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYR 120

Query: 121 KKHRKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAI 180
           KKHRKV+MKA K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN GEVKIGDLGLA 
Sbjct: 121 KKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLAT 180

Query: 181 VMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFK 240
           VM+Q  A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT EYPY EC+NSAQI+K
Sbjct: 181 VMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYK 240

Query: 241 KVTSGIKPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPF-----LATENPKELSS 300
           KV+SGIKPASL KV DP+V +FIEKCL+PAS R  A ELL D F     L   NP  L  
Sbjct: 241 KVSSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPD 300

Query: 301 ----EPSRLLNAQFKSVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQR 360
               +          S  PP   +  M ++ +      ++     SNN  S  + +E++R
Sbjct: 301 IVMPKEGSFGERCLMSEGPPNARNRTMSMNLD---EDNNLPIVISSNN--SGTNCIEVRR 360

Query: 361 FTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELS 420
               N   LKG+  D N++S  LRI ++ G+ +NIHF F+ + DTA  ++ EMVEQLEL+
Sbjct: 361 AKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELT 420

Query: 421 NEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSP 480
           +++   IAELID L+   +P+WK      H                              
Sbjct: 421 DKNVKFIAELIDVLLVNLIPNWKTDVAVDH------------------------------ 480

Query: 481 IVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGD-FSHPDCDKA 540
                   L+H      Q+NQ             S+  ++  G  S+ G+  SH      
Sbjct: 481 --------LIH-----PQQNQ-------------SSKDNHQNGASSQAGESISH------ 540

Query: 541 FSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEID 600
               ++ SD       + D A P++ ++      +         SLEE +  + LK E++
Sbjct: 541 ----SLSSD---YCPRSDDEANPTVAATTEDQEAE------KPGSLEEEEEDERLKEELE 551

Query: 601 AIDLQYHQCLCELSRMREEAIESVKKRWMSK 616
            I+ ++ + + E++R REEA    K R+  K
Sbjct: 601 KIEERFREEMKEITRKREEATMETKNRFFEK 551

BLAST of Sgr024498 vs. ExPASy TrEMBL
Match: A0A0A0KQP1 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5G148620 PE=4 SV=1)

HSP 1 Score: 1045.8 bits (2703), Expect = 7.1e-302
Identity = 531/626 (84.82%), Postives = 570/626 (91.05%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MSG+SEA+D  IVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 55  MSGNSEADDRGIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 114

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 115 SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKV 174

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 175 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 234

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 235 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 294

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPAS R  ASELLKDPFLA E+PK+ SSE SR LN  FK
Sbjct: 295 KPASLEKVLDPQVKQFIEKCLVPASTRLPASELLKDPFLAAESPKDNSSELSRSLNEHFK 354

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLLGSH M+ DHNC+K SGS+ SS KSNN ISHFST ELQR TENNELTLKGDMTD
Sbjct: 355 SVNPPLLGSHPMETDHNCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGDMTD 414

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 415 HNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 474

Query: 421 DKFVPSWKPCTNYC--HGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLT 480
            KFVPSWKPC NYC    +NT H S E QEDKTFI PF+SELVLSSP+VAAAR +L  L 
Sbjct: 475 AKFVPSWKPCPNYCEEQQQNTPH-SPEAQEDKTFISPFFSELVLSSPMVAAARNNLTGLA 534

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S++Y +ST SDYSIGKGSECG+F HPDC+KA+       ++++AV
Sbjct: 535 KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 594

Query: 541 GSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLC 600
           GS  TTIDFAKPSLISSCS +SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCLC
Sbjct: 595 GSLSTTIDFAKPSLISSCSEMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCLC 654

Query: 601 ELSRMREEAIESVKKRWMSKNKPSAV 622
           ELSRMREEAIES KKRWMSK K + +
Sbjct: 655 ELSRMREEAIESAKKRWMSKKKATGI 679

BLAST of Sgr024498 vs. ExPASy TrEMBL
Match: A0A5A7TLP6 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G001600 PE=4 SV=1)

HSP 1 Score: 1045.4 bits (2702), Expect = 9.3e-302
Identity = 530/627 (84.53%), Postives = 571/627 (91.07%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MS +SEA+D EIVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 9   MSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 68

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 69  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKV 128

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 129 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 188

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 189 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 248

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFL  E+PKE SSE SR LN QFK
Sbjct: 249 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQFK 308

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLLGSH MD DH C+K SGS+ SS KSNN ISHFST ELQR TENNELTLKG+MTD
Sbjct: 309 SVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMTD 368

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 369 HNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 428

Query: 421 DKFVPSWKPCTNYCHGE--NTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLT 480
            KFVPSWKPC NYC  E  NT  S SE QE+K+FI PF+SELVLSSP+V AAR +L  L 
Sbjct: 429 AKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGLA 488

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S++Y +ST SDYSIGKGSECG+F HPDC+KA+       ++++AV
Sbjct: 489 KVEDQENQQSIISCASVEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 548

Query: 541 GSAVTT-IDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCL 600
           GS ++T IDFAKPSLISSCSG+SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCL
Sbjct: 549 GSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCL 608

Query: 601 CELSRMREEAIESVKKRWMSKNKPSAV 622
           CELSRMREEAIES KKRWMSK K + +
Sbjct: 609 CELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Sgr024498 vs. ExPASy TrEMBL
Match: A0A1S3AUL5 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103482852 PE=4 SV=1)

HSP 1 Score: 1044.6 bits (2700), Expect = 1.6e-301
Identity = 530/627 (84.53%), Postives = 570/627 (90.91%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MS +SEA+D EIVEKDP+GRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 9   MSRNSEADDREIVEKDPSGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 68

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDK+KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 69  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKHKTINMITELFTSGSLRQYRKKHRKV 128

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT
Sbjct: 129 DLKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 188

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI
Sbjct: 189 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 248

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFL  E+PKE SSE SR LN QFK
Sbjct: 249 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVAESPKEHSSELSRSLNEQFK 308

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLLGSH MD DH C+K SGS+ SS KSNN ISHFST ELQR TENNELTLKG+MTD
Sbjct: 309 SVNPPLLGSHPMDTDHKCTKLSGSVASSVKSNNGISHFSTQELQRLTENNELTLKGEMTD 368

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HNT+SFHLRIAE  GKS+NIHFAFYLDSDT+LAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 369 HNTMSFHLRIAELYGKSRNIHFAFYLDSDTSLAIALEMVEQLELSNEDATIIAKLIDELI 428

Query: 421 DKFVPSWKPCTNYCHGE--NTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLT 480
            KFVPSWKPC NYC  E  NT  S SE QE+K+FI PF+SELVLSSP+V AAR +L  L 
Sbjct: 429 AKFVPSWKPCPNYCEEEQQNTPDSPSEAQEEKSFISPFFSELVLSSPMVVAARNNLTGLA 488

Query: 481 EVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEA---IESDAV 540
           +VEDQENQQSIISC S +Y +ST SDYSIGKGSECG+F HPDC+KA+       ++++AV
Sbjct: 489 KVEDQENQQSIISCASAEYIYSTVSDYSIGKGSECGEFGHPDCEKAYISSGTIDLDAEAV 548

Query: 541 GSAVTT-IDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCL 600
           GS ++T IDFAKPSLISSCSG+SK+LSLSS+S+ S+EERDH DELK+EIDAIDLQYHQCL
Sbjct: 549 GSTISTMIDFAKPSLISSCSGMSKELSLSSFSTLSMEERDHQDELKMEIDAIDLQYHQCL 608

Query: 601 CELSRMREEAIESVKKRWMSKNKPSAV 622
           CELSRMREEAIES KKRWMSK K + +
Sbjct: 609 CELSRMREEAIESAKKRWMSKKKATGI 635

BLAST of Sgr024498 vs. ExPASy TrEMBL
Match: A0A6J1DYE3 (Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=LOC111025697 PE=4 SV=1)

HSP 1 Score: 1040.0 bits (2688), Expect = 3.9e-300
Identity = 538/653 (82.39%), Postives = 564/653 (86.37%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MSG+SE ED E+ EKDPTGRYIRYDE+LGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ
Sbjct: 4   MSGNSEPEDREVAEKDPTGRYIRYDEILGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 63

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKF+SYWVDD+NKTINMITELFTSGSLRQYRKKHRKV
Sbjct: 64  SPEQLQRLYSEVHLLKSLKHENIIKFHSYWVDDENKTINMITELFTSGSLRQYRKKHRKV 123

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQ T
Sbjct: 124 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQST 183

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKN+AQIFKKVTSG 
Sbjct: 184 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNAAQIFKKVTSGT 243

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
           KPASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFL  EN KE SSEPSRLLNAQF 
Sbjct: 244 KPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLVIENTKEHSSEPSRLLNAQFI 303

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFST-------------------- 360
           SVNPPLLGS+ +DI H+C K SGS  SSAKS+NEISHFST                    
Sbjct: 304 SVNPPLLGSNPVDIAHSCMKLSGSESSSAKSSNEISHFSTPELSAKSSNEISHFSTLELT 363

Query: 361 ------------LELQRFTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDS 420
                       LELQRFTENNEL LKG+MTDHNTISFHLRIAEQCGKSKNIHFAFYLDS
Sbjct: 364 AKSINEVSHFSSLELQRFTENNELMLKGEMTDHNTISFHLRIAEQCGKSKNIHFAFYLDS 423

Query: 421 DTALAIALEMVEQLELSNEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQE 480
           DTALAIALEMVEQLELSNEDAT IA+LIDELI KFVPSWKPC N C         SETQE
Sbjct: 424 DTALAIALEMVEQLELSNEDATTIAKLIDELITKFVPSWKPCANIC---------SETQE 483

Query: 481 DKTFIPPFYSELVLSSPIVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIG 540
           DKTFIPPF+ ELVLSSP+VA ARKSL HL ++EDQ+NQQSIISC SLDY HST S YS G
Sbjct: 484 DKTFIPPFFPELVLSSPMVADARKSLTHLADMEDQDNQQSIISCASLDYIHSTTSGYSTG 543

Query: 541 KGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSF 600
           KGSE GDF HP+ +K F  E I+SD   S  T IDFAKPSLISSCSG+SKDLSLSS+SSF
Sbjct: 544 KGSEYGDFGHPEGEKVFISEPIDSDTAVSVSTVIDFAKPSLISSCSGMSKDLSLSSFSSF 603

Query: 601 SLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMSKNKPSAV 622
           S+EERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWM+K K  A+
Sbjct: 604 SVEERDHHDELKLEIDAIDLQYHQCLCELSRMREEAIESVKKRWMAKKKACAI 647

BLAST of Sgr024498 vs. ExPASy TrEMBL
Match: A0A6J1H579 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111459693 PE=4 SV=1)

HSP 1 Score: 1038.9 bits (2685), Expect = 8.7e-300
Identity = 525/625 (84.00%), Postives = 569/625 (91.04%), Query Frame = 0

Query: 1   MSGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQ 60
           MSG+SEA+D  I+EKDPTGRYIRYDE+LGKGAFKTV+KAFD+ GGIEVAWSQVDIEDVLQ
Sbjct: 8   MSGNSEADDLNILEKDPTGRYIRYDEILGKGAFKTVFKAFDKAGGIEVAWSQVDIEDVLQ 67

Query: 61  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKV 120
           SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDD  KTINMITELFTSGSLRQYRKKHRKV
Sbjct: 68  SPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDNTKTINMITELFTSGSLRQYRKKHRKV 127

Query: 121 DMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPT 180
           D+KAFKNWAR+ILRGLTYLHG +PPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIV+QQPT
Sbjct: 128 DLKAFKNWARKILRGLTYLHGQDPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVLQQPT 187

Query: 181 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGI 240
           ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSG+
Sbjct: 188 ARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGV 247

Query: 241 KPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFK 300
            PASL+KVLDPQVKQFIEKCLVPASMR  ASELLKDPFLATEN K+ SSEPS LLNAQF+
Sbjct: 248 MPASLEKVLDPQVKQFIEKCLVPASMRLPASELLKDPFLATENRKDHSSEPSMLLNAQFE 307

Query: 301 SVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTD 360
           SVNPPLL ++ MD+DHNCSK SGS+ SS +SNNEISHFST EL+  TENNELTLKGDMTD
Sbjct: 308 SVNPPLLDTNPMDMDHNCSKLSGSVASSIESNNEISHFSTQELKTLTENNELTLKGDMTD 367

Query: 361 HNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELI 420
           HN+ISFHLRIAE  G+SKNIHFAF+LDSDTALAIALEMVEQLELSNEDATIIA+LIDELI
Sbjct: 368 HNSISFHLRIAELSGQSKNIHFAFFLDSDTALAIALEMVEQLELSNEDATIIAQLIDELI 427

Query: 421 DKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLTEV 480
            KFVPSW+ CTN C G  T HSSSET+EDK F PP +SE+VLSSP+V A RK+L  L EV
Sbjct: 428 AKFVPSWQQCTNCCDGLITEHSSSETEEDKAFSPPLFSEMVLSSPLVGAGRKTLTELAEV 487

Query: 481 EDQENQQSIISCVSLDYNHSTASDYSIGK-GSECGDFSHPDCDKAF--SGEAI-ESDAVG 540
           E QENQQ+IISC SLDYNHST SDYS+GK GSECGDFSHP+CDKAF  SG    + DAVG
Sbjct: 488 EHQENQQTIISCASLDYNHSTISDYSLGKGGSECGDFSHPECDKAFISSGSGTSDLDAVG 547

Query: 541 SAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLCE 600
           S  T  DFAKPSLISSCSG+SK+LSLS++S+FS+EERD HDELK+EIDAID+QYHQCLCE
Sbjct: 548 SVSTMFDFAKPSLISSCSGMSKELSLSNFSNFSVEERDQHDELKMEIDAIDMQYHQCLCE 607

Query: 601 LSRMREEAIESVKKRWMSKNKPSAV 622
           LSRMREEAIES KKRWMSK K +A+
Sbjct: 608 LSRMREEAIESAKKRWMSKKKGAAI 632

BLAST of Sgr024498 vs. TAIR 10
Match: AT5G41990.1 (with no lysine (K) kinase 8 )

HSP 1 Score: 583.9 bits (1504), Expect = 1.5e-166
Identity = 324/613 (52.85%), Postives = 415/613 (67.70%), Query Frame = 0

Query: 5   SEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQ 64
           S  E+ +  EKDP+GRYIRYD+VLG+GAFKTVYKAFDEV GIEVAW+ V IEDV+Q P Q
Sbjct: 12  SSMEEADFAEKDPSGRYIRYDDVLGRGAFKTVYKAFDEVDGIEVAWNLVSIEDVMQMPGQ 71

Query: 65  LQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVDMKA 124
           L+RLYSEVHLLK+LKHENIIK +  WVD+KNKTINMITELFTSGSLR YRKKHRKVD KA
Sbjct: 72  LERLYSEVHLLKALKHENIIKLFYSWVDEKNKTINMITELFTSGSLRVYRKKHRKVDPKA 131

Query: 125 FKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSV 184
            KNWARQIL+GL YLH  NPP+IHRDLKCDNIFVNGNTGEVKIGDLGLA V+QQPTARSV
Sbjct: 132 IKNWARQILKGLNYLHSQNPPVIHRDLKCDNIFVNGNTGEVKIGDLGLATVLQQPTARSV 191

Query: 185 IGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPAS 244
           IGTPEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPYNEC+N AQI+KKVTS IKP S
Sbjct: 192 IGTPEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYNECRNQAQIYKKVTSNIKPQS 251

Query: 245 LDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFKSVNP 304
           L KV DPQV+QFIEKCL+PAS RP A EL KDPFLA +  K+ +   S   ++++  V P
Sbjct: 252 LGKVDDPQVRQFIEKCLLPASSRPTALELSKDPFLARDGGKDSALLASSSTSSKY--VRP 311

Query: 305 PLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTDHNTI 364
           P L    MD+DHN +K       S  SN +     T+ELQR  EN E  L+G+ +D  T 
Sbjct: 312 PQLEHLPMDVDHNENK-------SVSSNEDYPWSQTIELQRIAENKEFRLRGERSDDVTA 371

Query: 365 SFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELIDKFV 424
           S  LRIA+  GK + +HFAFYL+SDTA AIA EMVE+L L++++  +IA++ID+ I + +
Sbjct: 372 SMVLRIADPSGKCRIVHFAFYLESDTATAIAEEMVEELHLTSQEVVVIADMIDDFIMQLL 431

Query: 425 PSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLTEVEDQE 484
                 T+  H +N+   + E  E                   AA +++   +   +++ 
Sbjct: 432 ---SDRTSSHHNQNSPRLTHEDHE-------------------AANQQT---VNSKDEEA 491

Query: 485 NQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDF 544
             QS+ S +S DY       YS   G+   +          +G   ES            
Sbjct: 492 AGQSMKSDISADYYF----PYSANDGNAAME----------AGRDAES------------ 551

Query: 545 AKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEA 604
              S + SCS       +S+  + S+ + D+ ++LK E++ I+ Q++Q   +L +++E+A
Sbjct: 552 -MSSYLDSCS------MMSTIYNLSISDNDYPEDLKTELNLIESQFNQSFQDLLKLKEDA 557

Query: 605 IESVKKRWMSKNK 618
           IE+ K++W++K +
Sbjct: 612 IENAKRKWITKKQ 557

BLAST of Sgr024498 vs. TAIR 10
Match: AT1G64630.1 (with no lysine (K) kinase 10 )

HSP 1 Score: 557.8 bits (1436), Expect = 1.1e-158
Identity = 320/609 (52.55%), Postives = 385/609 (63.22%), Query Frame = 0

Query: 8   EDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQSPEQLQR 67
           E+ + V+KDPTGRYIRY++VLG+GAFKTVYKAFDEV GIEVAW+ + IEDVLQ P QL R
Sbjct: 2   EEADFVQKDPTGRYIRYNDVLGRGAFKTVYKAFDEVEGIEVAWNLMSIEDVLQMPGQLDR 61

Query: 68  LYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVDMKAFKN 127
           LYSEVHLL SLKH+NIIK +  WVDD NK+INMITELFTSGSL  YRKKHRKVD KA  N
Sbjct: 62  LYSEVHLLNSLKHDNIIKLFYSWVDDHNKSINMITELFTSGSLTLYRKKHRKVDPKAIMN 121

Query: 128 WARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTARSVIGT 187
           WARQIL+GL YLH   PP+IHRDLKCDNIFVNGNTG+VKIGDLGLA VMQQPTARSVIGT
Sbjct: 122 WARQILKGLHYLHSQTPPVIHRDLKCDNIFVNGNTGKVKIGDLGLAAVMQQPTARSVIGT 181

Query: 188 PEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIKPASLDK 247
           PEFMAPELY+E+YNELVDIYSFGMCMLE+VTCEYPY EC+N AQI+KKVTSGIKP SL K
Sbjct: 182 PEFMAPELYEEEYNELVDIYSFGMCMLEMVTCEYPYRECRNQAQIYKKVTSGIKPQSLSK 241

Query: 248 VLDPQVKQFIEKCLVPASMRPFASELLKDPFLATENPKELSSEPSRLLNAQFKSVNPPLL 307
           V DPQVKQFIEKCL+PA  RP A ELLKD  LA +  K+ +   S   N  FK   PP  
Sbjct: 242 VDDPQVKQFIEKCLLPAPSRPTALELLKDQLLAVDGAKDSTLTASS--NTTFKPAMPPQC 301

Query: 308 GSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQRFTENNELTLKGDMTDHNTISFH 367
               MD+++     S S+CSSAKS+ E +   T+E+QR  E+ E  L G+  D    S  
Sbjct: 302 EYRPMDVEYK-KNTSVSICSSAKSSQECALLQTMEVQRVAESTEFKLSGERRDDVAASMA 361

Query: 368 LRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELSNEDATIIAELIDELIDKFVPSW 427
           LRIA   G+++ + F F L +DTA A+  EMVE+L+LS+ + T+IAE+IDELI K     
Sbjct: 362 LRIAGSSGQARKVDFDFNLKTDTARAVTGEMVEELDLSSHEVTVIAEMIDELIMKL---- 421

Query: 428 KPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSPIVAAARKSLLHLTEV---EDQE 487
                                                     A +SL +   V   +D+E
Sbjct: 422 -----------------------------------------KANRSLPNANSVYQSKDEE 481

Query: 488 NQQSIISCVSLDYNHSTASDYSIGKGSECGDFSHPDCDKAFSGEAIESDAVGSAVTTIDF 547
             +S+ S +S DY H  +S+     G  C              EA+ES            
Sbjct: 482 AGESMKSEISADYYHRVSSNEGSRLGCCC--------------EAVES------------ 521

Query: 548 AKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEIDAIDLQYHQCLCELSRMREEA 607
              S + SCS VS   S               ++LK E++ I+ QY+Q    L RM+EEA
Sbjct: 542 LLSSFLDSCSMVSNKQS---------------EDLKTELNVIESQYNQSCQRLLRMKEEA 521

Query: 608 IESVKKRWM 614
           IE  K++WM
Sbjct: 602 IEKAKRKWM 521

BLAST of Sgr024498 vs. TAIR 10
Match: AT3G18750.1 (with no lysine (K) kinase 6 )

HSP 1 Score: 518.1 bits (1333), Expect = 9.9e-147
Identity = 307/631 (48.65%), Postives = 380/631 (60.22%), Query Frame = 0

Query: 2   SGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQS 61
           S   E  D E++E DPT RYIRY EV+GKGAFKTVYKAFDEV GIEVAW+QV I+DVLQS
Sbjct: 8   SALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQS 67

Query: 62  PEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVD 121
           P  L+RLYSEV LLKSLKH NII+FY+ W+DDKNKT+N+ITELFTSGSLR YRKKHRKV+
Sbjct: 68  PNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVN 127

Query: 122 MKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTA 181
           MKA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q  A
Sbjct: 128 MKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQANA 187

Query: 182 RSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIK 241
           +SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV+SGIK
Sbjct: 188 KSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIK 247

Query: 242 PASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATEN--------------PKEL 301
           PASL +V DP+VKQFIEKCL+PAS R  A ELL DPFL                  PKE 
Sbjct: 248 PASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKEG 307

Query: 302 SSEPSRLLNAQFKSVNPPLLGSHLMDIDHNC-----SKRSGSMCSSAKSNNEISHFSTLE 361
           +     L++    +  P    S  +D D N      S  SGS C              +E
Sbjct: 308 AFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRC--------------IE 367

Query: 362 LQRFTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQL 421
           ++R    N   LKG+  D  ++S  LRI ++ G+ +NIHF FY + DTA  ++ EMVEQL
Sbjct: 368 VRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQL 427

Query: 422 ELSNEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVL 481
           EL++++ T IAELID L+   +P+WK                    D T     +S+L  
Sbjct: 428 ELTDQNVTFIAELIDILLVNMIPTWK-------------------TDVTVDHLIHSQL-- 487

Query: 482 SSPIVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIGKGSEC--GDFSHPD 541
                   + S  H  E + Q+ ++++         H T    S    S+C   D     
Sbjct: 488 -------NQNSRSHHNEAKPQKQEETVF--------HDTCELVSHSCNSDCPRSDEEDKQ 547

Query: 542 CDKAFSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELK 601
           C  A  GE                   S I      ++ +SL           +  + L+
Sbjct: 548 CVDATKGE-----------------DKSSIQEVEEATEPVSL-----------EEEERLR 560

Query: 602 LEIDAIDLQYHQCLCELSRMREEAIESVKKR 612
            E++ I+ +Y + + E++  REEAI   KK+
Sbjct: 608 QELEEIEAKYQEDMKEIATKREEAIMETKKK 560

BLAST of Sgr024498 vs. TAIR 10
Match: AT3G18750.3 (with no lysine (K) kinase 6 )

HSP 1 Score: 518.1 bits (1333), Expect = 9.9e-147
Identity = 307/631 (48.65%), Postives = 380/631 (60.22%), Query Frame = 0

Query: 2   SGSSEAEDCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVDIEDVLQS 61
           S   E  D E++E DPT RYIRY EV+GKGAFKTVYKAFDEV GIEVAW+QV I+DVLQS
Sbjct: 8   SALQEPPDPEVLEVDPTFRYIRYKEVIGKGAFKTVYKAFDEVDGIEVAWNQVRIDDVLQS 67

Query: 62  PEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYRKKHRKVD 121
           P  L+RLYSEV LLKSLKH NII+FY+ W+DDKNKT+N+ITELFTSGSLR YRKKHRKV+
Sbjct: 68  PNCLERLYSEVRLLKSLKHNNIIRFYNSWIDDKNKTVNIITELFTSGSLRHYRKKHRKVN 127

Query: 122 MKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAIVMQQPTA 181
           MKA KNWARQIL GL YLHG  PPIIHRDLKCDNIF+NGN GEVKIGDLGLA VM+Q  A
Sbjct: 128 MKAVKNWARQILMGLRYLHGQEPPIIHRDLKCDNIFINGNHGEVKIGDLGLATVMEQANA 187

Query: 182 RSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFKKVTSGIK 241
           +SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT +YPY ECKNSAQI+KKV+SGIK
Sbjct: 188 KSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFDYPYCECKNSAQIYKKVSSGIK 247

Query: 242 PASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPFLATEN--------------PKEL 301
           PASL +V DP+VKQFIEKCL+PAS R  A ELL DPFL                  PKE 
Sbjct: 248 PASLSRVKDPEVKQFIEKCLLPASERLSAKELLLDPFLQLNGLTMNNPLPLPDIVMPKEG 307

Query: 302 SSEPSRLLNAQFKSVNPPLLGSHLMDIDHNC-----SKRSGSMCSSAKSNNEISHFSTLE 361
           +     L++    +  P    S  +D D N      S  SGS C              +E
Sbjct: 308 AFGDRCLMSEGPPTTRPSKTLSIDLDEDSNLPIVTFSDNSGSRC--------------IE 367

Query: 362 LQRFTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQL 421
           ++R    N   LKG+  D  ++S  LRI ++ G+ +NIHF FY + DTA  ++ EMVEQL
Sbjct: 368 VRRAKRGNFFVLKGEENDEQSVSLILRIVDENGRVRNIHFLFYQEGDTASKVSSEMVEQL 427

Query: 422 ELSNEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVL 481
           EL++++ T IAELID L+   +P+WK                    D T     +S+L  
Sbjct: 428 ELTDQNVTFIAELIDILLVNMIPTWK-------------------TDVTVDHLIHSQL-- 487

Query: 482 SSPIVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIGKGSEC--GDFSHPD 541
                   + S  H  E + Q+ ++++         H T    S    S+C   D     
Sbjct: 488 -------NQNSRSHHNEAKPQKQEETVF--------HDTCELVSHSCNSDCPRSDEEDKQ 547

Query: 542 CDKAFSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELK 601
           C  A  GE                   S I      ++ +SL           +  + L+
Sbjct: 548 CVDATKGE-----------------DKSSIQEVEEATEPVSL-----------EEEERLR 560

Query: 602 LEIDAIDLQYHQCLCELSRMREEAIESVKKR 612
            E++ I+ +Y + + E++  REEAI   KK+
Sbjct: 608 QELEEIEAKYQEDMKEIATKREEAIMETKKK 560

BLAST of Sgr024498 vs. TAIR 10
Match: AT1G49160.2 (Protein kinase superfamily protein )

HSP 1 Score: 492.3 bits (1266), Expect = 5.8e-139
Identity = 297/631 (47.07%), Postives = 383/631 (60.70%), Query Frame = 0

Query: 1   MSGSSEAE------DCEIVEKDPTGRYIRYDEVLGKGAFKTVYKAFDEVGGIEVAWSQVD 60
           M GS +A       D E++E DPT RYIRY EV+GKGA KTV+K FDEV GIEVAW+QV 
Sbjct: 1   MEGSEDASAIVEPPDPEVLEIDPTCRYIRYKEVIGKGASKTVFKGFDEVDGIEVAWNQVR 60

Query: 61  IEDVLQSPEQLQRLYSEVHLLKSLKHENIIKFYSYWVDDKNKTINMITELFTSGSLRQYR 120
           I+D+LQSP+ L+RLYSEV LLKSLKH+NII+FY+ W+DDKNKT+N+ITELFTSGSLRQYR
Sbjct: 61  IDDLLQSPDCLERLYSEVRLLKSLKHKNIIRFYNSWIDDKNKTVNIITELFTSGSLRQYR 120

Query: 121 KKHRKVDMKAFKNWARQILRGLTYLHGHNPPIIHRDLKCDNIFVNGNTGEVKIGDLGLAI 180
           KKHRKV+MKA K WARQIL GL YLH  +PPIIHRD+KCDNIF+NGN GEVKIGDLGLA 
Sbjct: 121 KKHRKVNMKAVKCWARQILTGLKYLHSQDPPIIHRDIKCDNIFINGNHGEVKIGDLGLAT 180

Query: 181 VMQQPTARSVIGTPEFMAPELYDEDYNELVDIYSFGMCMLEIVTCEYPYNECKNSAQIFK 240
           VM+Q  A+SVIGTPEFMAPELYDE+YNEL DIYSFGMCMLE+VT EYPY EC+NSAQI+K
Sbjct: 181 VMEQANAKSVIGTPEFMAPELYDENYNELADIYSFGMCMLEMVTFEYPYCECRNSAQIYK 240

Query: 241 KVTSGIKPASLDKVLDPQVKQFIEKCLVPASMRPFASELLKDPF-----LATENPKELSS 300
           KV+SGIKPASL KV DP+V +FIEKCL+PAS R  A ELL D F     L   NP  L  
Sbjct: 241 KVSSGIKPASLSKVKDPEVMKFIEKCLLPASERLSAEELLLDSFLNVNGLVMNNPLPLPD 300

Query: 301 ----EPSRLLNAQFKSVNPPLLGSHLMDIDHNCSKRSGSMCSSAKSNNEISHFSTLELQR 360
               +          S  PP   +  M ++ +      ++     SNN  S  + +E++R
Sbjct: 301 IVMPKEGSFGERCLMSEGPPNARNRTMSMNLD---EDNNLPIVISSNN--SGTNCIEVRR 360

Query: 361 FTENNELTLKGDMTDHNTISFHLRIAEQCGKSKNIHFAFYLDSDTALAIALEMVEQLELS 420
               N   LKG+  D N++S  LRI ++ G+ +NIHF F+ + DTA  ++ EMVEQLEL+
Sbjct: 361 AKRGNFFVLKGEENDENSVSLILRIVDENGRVRNIHFLFFQEGDTASNVSSEMVEQLELT 420

Query: 421 NEDATIIAELIDELIDKFVPSWKPCTNYCHGENTAHSSSETQEDKTFIPPFYSELVLSSP 480
           +++   IAELID L+   +P+WK      H                              
Sbjct: 421 DKNVKFIAELIDVLLVNLIPNWKTDVAVDH------------------------------ 480

Query: 481 IVAAARKSLLHLTEVEDQENQQSIISCVSLDYNHSTASDYSIGKGSECGD-FSHPDCDKA 540
                   L+H      Q+NQ             S+  ++  G  S+ G+  SH      
Sbjct: 481 --------LIH-----PQQNQ-------------SSKDNHQNGASSQAGESISH------ 540

Query: 541 FSGEAIESDAVGSAVTTIDFAKPSLISSCSGVSKDLSLSSYSSFSLEERDHHDELKLEID 600
               ++ SD       + D A P++ ++      +         SLEE +  + LK E++
Sbjct: 541 ----SLSSD---YCPRSDDEANPTVAATTEDQEAE------KPGSLEEEEEDERLKEELE 551

Query: 601 AIDLQYHQCLCELSRMREEAIESVKKRWMSK 616
            I+ ++ + + E++R REEA    K R+  K
Sbjct: 601 KIEERFREEMKEITRKREEATMETKNRFFEK 551

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038874814.11.7e-30284.98serine/threonine-protein kinase WNK8-like isoform X1 [Benincasa hispida][more]
XP_004143946.11.5e-30184.82serine/threonine-protein kinase WNK8 isoform X1 [Cucumis sativus] >KGN49991.1 hy... [more]
KAA0042657.11.9e-30184.53serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo var. makuwa] ... [more]
XP_008437436.13.3e-30184.53PREDICTED: serine/threonine-protein kinase WNK8-like isoform X1 [Cucumis melo][more]
XP_022159288.18.1e-30082.39probable serine/threonine-protein kinase WNK10 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q944Q02.1e-16552.85Serine/threonine-protein kinase WNK8 OS=Arabidopsis thaliana OX=3702 GN=WNK8 PE=... [more]
Q6EU494.9e-15951.76Probable serine/threonine-protein kinase WNK4 OS=Oryza sativa subsp. japonica OX... [more]
Q8RXE51.6e-15752.55Probable serine/threonine-protein kinase WNK10 OS=Arabidopsis thaliana OX=3702 G... [more]
Q8S8Y81.4e-14548.65Probable serine/threonine-protein kinase WNK6 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q8LST28.2e-13847.07Probable serine/threonine-protein kinase WNK7 OS=Arabidopsis thaliana OX=3702 GN... [more]
Match NameE-valueIdentityDescription
A0A0A0KQP17.1e-30284.82Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_5... [more]
A0A5A7TLP69.3e-30284.53Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A1S3AUL51.6e-30184.53Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A6J1DYE33.9e-30082.39Non-specific serine/threonine protein kinase OS=Momordica charantia OX=3673 GN=L... [more]
A0A6J1H5798.7e-30084.00Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
Match NameE-valueIdentityDescription
AT5G41990.11.5e-16652.85with no lysine (K) kinase 8 [more]
AT1G64630.11.1e-15852.55with no lysine (K) kinase 10 [more]
AT3G18750.19.9e-14748.65with no lysine (K) kinase 6 [more]
AT3G18750.39.9e-14748.65with no lysine (K) kinase 6 [more]
AT1G49160.25.8e-13947.07Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 22..279
e-value: 2.2E-60
score: 216.6
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 25..279
e-value: 1.7E-51
score: 175.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 22..279
score: 41.926403
NoneNo IPR availableGENE3D3.10.20.90coord: 362..460
e-value: 1.9E-7
score: 33.3
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 105..286
e-value: 6.0E-52
score: 177.8
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 5..103
e-value: 7.1E-37
score: 127.4
NoneNo IPR availablePIRSRPIRSR000556-2PIRSR000556-2coord: 20..228
e-value: 7.2E-26
score: 88.6
NoneNo IPR availablePIRSRPIRSR633573-1PIRSR633573-1coord: 26..217
e-value: 8.0E-13
score: 46.1
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 9..282
e-value: 2.5E-27
score: 93.4
NoneNo IPR availablePANTHERPTHR13902:SF139WNK KINASEcoord: 5..616
NoneNo IPR availablePANTHERPTHR13902SERINE/THREONINE-PROTEIN KINASE WNK WITH NO LYSINE -RELATEDcoord: 5..616
NoneNo IPR availableCDDcd13983STKc_WNKcoord: 20..279
e-value: 1.10688E-170
score: 484.037
IPR024678Serine/threonine-protein kinase OSR1/WNK, CCT domainPFAMPF12202OSR1_Ccoord: 365..421
e-value: 5.8E-6
score: 26.2
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 146..158
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 16..279

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr024498.1Sgr024498.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity