Sgr024350 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr024350 vs. NCBI nr
Match: KAA0042821.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 936.0 bits (2418), Expect = 1.6e-268
Identity = 479/669 (71.60%), Postives = 546/669 (81.61%), Query Frame = 0

Query: 15  QLQTYIVHVKQPEIRLLGEETND-IESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSAR 74
           +LQTYIVHV+QP+IRL+GE +ND IESWYMSF+P++TE ++EQP+LLYSYRNVMSGFSAR
Sbjct: 33  KLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSAR 92

Query: 75  LTEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDT 134
           LT EQV+AME KDGFISA PETI+SLHTTHTP +LGLNQQFG+WK+SNFG+GVI+GVLDT
Sbjct: 93  LTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDT 152

Query: 135 GITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGH 194
           GI P HPSF DEGMSPPPAKWKG+CEFNSS+CNNKLIGARTFN  N     ESP DE GH
Sbjct: 153 GIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGH 212

Query: 195 GTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVD 254
           GTHTA TAAGAFV+ AEALGNA+G A G+APLAHLA+YKVCS K C SSDV AG+DAA+D
Sbjct: 213 GTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAID 272

Query: 255 DGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVV 314
           DGVDVLS+SLGSRS PFFKDN+A+ TF AIQK                       ++L V
Sbjct: 273 DGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTV 332

Query: 315 RPE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTD 374
                                      QP+DF S FLPLVYAG+  N+T AFCG+GSL +
Sbjct: 333 GASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLEN 392

Query: 375 IDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYE 434
           +DVKGKVVVC+G+GGVGR+AKG VVKNAGGAAMILINQ+EDGF+T SEAHVLP +HVSY+
Sbjct: 393 MDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYK 452

Query: 435 AGLKIKAYISSSQNPTASISFKGTIIGDGS--SSPAMASFSSRGPSLSSPGILKPDITGP 494
           AG+ IK+YI+SSQNPTASISFKGT+IGDG   S+P+MASFSSRGP L SPGILKPDITGP
Sbjct: 453 AGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGP 512

Query: 495 GVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS-YPN------------ 554
           GVNILAAWPFPLDN+T+T+STFNVISGTSMSCPHLSGIAALIKS +PN            
Sbjct: 513 GVNILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 572

Query: 555 ------PEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKDA 614
                 P+G+PIVDQDL+PAN FA+GAGHV+PS+AADPGLVYDI+PDDYIPYLCGLYKD 
Sbjct: 573 TANIKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 632

Query: 615 EVSIIVQKPVICSLVPRIREGDLNYPSFSVALGPLQTFKRTVTNVGDANSVYSAIIEAPL 617
           EVSIIV + VIC LVP IREGDLNYPSFSVALG LQTFKRTVTNVG+ANSVY+AI+EAPL
Sbjct: 633 EVSIIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPL 692

BLAST of Sgr024350 vs. NCBI nr
Match: XP_008437722.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 930.6 bits (2404), Expect = 6.9e-267
Identity = 477/669 (71.30%), Postives = 544/669 (81.32%), Query Frame = 0

Query: 15  QLQTYIVHVKQPEIRLLGEETND-IESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSAR 74
           +LQTYIVHV+QP+IRL+GE +ND IESWYMSF+P++TE ++EQP+LLYSYRNVMSGFSAR
Sbjct: 42  KLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSAR 101

Query: 75  LTEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDT 134
           LT EQV+AME KDGFISA PETI+SLHTTHTP +LGLNQQFG+WK+SNFG+GVI+GVLDT
Sbjct: 102 LTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDT 161

Query: 135 GITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGH 194
           GI P HPSF DEGMSPPPAKWKG+CEFNSS+CNNKLIGARTFN  N     ESP DE GH
Sbjct: 162 GIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGH 221

Query: 195 GTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVD 254
           GTHTA TAAGAFV+ AEALGNA+G A G+APLAHLA+YKVCS K C SSDV AG+DAA+D
Sbjct: 222 GTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAID 281

Query: 255 DGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVV 314
           DGVDVLS+SLGSRS PFFKDN+A+ TF AIQK                       ++L V
Sbjct: 282 DGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTV 341

Query: 315 RPE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTD 374
                                      QP+DF S FLPLVYAG+  N+T AFCG+GSL +
Sbjct: 342 GASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLEN 401

Query: 375 IDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYE 434
           +DVKGKVVVC+G+GGVGR+AKG VVKNAGGAAMILINQ+EDGF+T SEAHVLP +HVSY+
Sbjct: 402 MDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYK 461

Query: 435 AGLKIKAYISSSQNPTASISFKGTIIGDGS--SSPAMASFSSRGPSLSSPGILKPDITGP 494
           AG+ IK+YI+SSQNPTASISFKGT+IGDG   S+P+MASFSSRGP L SPGILKPDITGP
Sbjct: 462 AGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGP 521

Query: 495 GVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS-YPN------------ 554
           GVNILAAWPFPLDN+T+T+STFNVISGTSMSCPHLSGIAALIKS +PN            
Sbjct: 522 GVNILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 581

Query: 555 ------PEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKDA 614
                 P+G+PI DQDL+PAN FA+GAGHV+PS+AADPGLVYDI+PDDYIPYLCGLYKD 
Sbjct: 582 TANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 641

Query: 615 EVSIIVQKPVICSLVPRIREGDLNYPSFSVALGPLQTFKRTVTNVGDANSVYSAIIEAPL 617
           EVSIIV + VIC LV  IREGDLNYPSFSVALG LQTFKRTVTNVG+ANSVY+AI+EAPL
Sbjct: 642 EVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPL 701

BLAST of Sgr024350 vs. NCBI nr
Match: XP_038906452.1 (subtilisin-like protease 4 [Benincasa hispida])

HSP 1 Score: 889.8 bits (2298), Expect = 1.3e-254
Identity = 465/669 (69.51%), Postives = 528/669 (78.92%), Query Frame = 0

Query: 15  QLQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARL 74
           +LQTYIVHVK+ E+R+LG+   D+ESWY SFLPET + S EQ RLLYS+RNVMSGFSARL
Sbjct: 13  KLQTYIVHVKESELRILGDAI-DLESWYTSFLPETIKTSNEQSRLLYSFRNVMSGFSARL 72

Query: 75  TEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTG 134
           TEE V+AME K+GFISA PETIL+LHTTHTP +LGLNQQFGLWKDSNFG+GVIIGVLDTG
Sbjct: 73  TEE-VKAMEEKEGFISARPETILNLHTTHTPEYLGLNQQFGLWKDSNFGKGVIIGVLDTG 132

Query: 135 ITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGHG 194
           I+P HPSF D+GM  PPAKWKGRCEF +S+CNNKLIGARTFNRAN    GESP DENGHG
Sbjct: 133 ISPDHPSFNDDGMPQPPAKWKGRCEFGASICNNKLIGARTFNRANSALMGESPIDENGHG 192

Query: 195 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 254
           THTA TAAG FV+GAEALGNA G AVGMAPLAHLAMYKVCS KGCSSSD+LA LDAA+ D
Sbjct: 193 THTASTAAGTFVEGAEALGNARGKAVGMAPLAHLAMYKVCSPKGCSSSDILAALDAAIHD 252

Query: 255 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQ---------------KEFLLVVRPEI---- 314
           GVDVLSLSLG+ S PF+ DN+AIG FAAI+               K  L    P I    
Sbjct: 253 GVDVLSLSLGAPSRPFYIDNIAIGAFAAIKMGIFVSCSAGNSGPSKSTLANEAPWILTVG 312

Query: 315 --------------------------QPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTDI 374
                                     QP DF S+FLPLVYAGS+G + S FC QGSLT++
Sbjct: 313 ASTIDRTIMALARLGNGEVHVGESLFQPRDFPSSFLPLVYAGSSGVKGSEFCVQGSLTNL 372

Query: 375 DVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYEA 434
           +VKGK+VVC+  GG+GRIAKG VVKNAGGAAMI+INQ+ D F+T +EAH+LP +HVSYEA
Sbjct: 373 NVKGKMVVCERGGGIGRIAKGMVVKNAGGAAMIIINQKADEFSTLAEAHILPATHVSYEA 432

Query: 435 GLKIKAYISSSQNPTASISFKGTIIGDGSS--SPAMASFSSRGPSLSSPGILKPDITGPG 494
           GLKIK+YI SSQNP ASISF+GT+IG  ++  SPAMASFSSRGP L SPGILKPDITGPG
Sbjct: 433 GLKIKSYIRSSQNPMASISFEGTLIGSRATTFSPAMASFSSRGPCLPSPGILKPDITGPG 492

Query: 495 VNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS-YP-------------- 554
           VNILAAWPF LDNNT+ +STFNVISGTSMSCPHLSGIAALIKS +P              
Sbjct: 493 VNILAAWPFSLDNNTNKKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMTS 552

Query: 555 ----NPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKDAE 614
               N +G+PIVDQDLKPAN FA+G+GHV+PS+AADPGLVYDI+PDDYIPYLCGLY DA+
Sbjct: 553 ADVRNRQGRPIVDQDLKPANFFAMGSGHVNPSKAADPGLVYDIQPDDYIPYLCGLYSDAQ 612

Query: 615 VSIIVQKPVICSLVPRIREGDLNYPSFSVALGPLQTFKRTVTNVGDANSVYSAIIEAPLG 618
           VSIIV++ V CS + RIREGDLNYPSF+V LGP Q F RTVTNVGDANS+YSAI+EAP+G
Sbjct: 613 VSIIVRRKVTCSTISRIREGDLNYPSFAVTLGPSQMFNRTVTNVGDANSIYSAIVEAPIG 672

BLAST of Sgr024350 vs. NCBI nr
Match: XP_008437177.1 (PREDICTED: subtilisin-like protease SBT1.7 [Cucumis melo])

HSP 1 Score: 865.5 bits (2235), Expect = 2.7e-247
Identity = 455/672 (67.71%), Postives = 522/672 (77.68%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTE-NSIEQPRLLYSYRNVMSGFSARL 75
           LQTYIVHVKQP + +LG +T D+++WY SFLPET E +S EQPRLLYSYR+VMSGFSARL
Sbjct: 33  LQTYIVHVKQPVLEILG-DTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARL 92

Query: 76  TEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTG 135
           T+EQV+AME KDGFISA PET L+LHTTHTP +LGLN+ FGLWK+SNFG+GVIIGVLDTG
Sbjct: 93  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTG 152

Query: 136 ITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
           I P HPSF DEGMS PPAKWKGRCEF +S+CNNKLIGARTFN AN    G+SP DENGHG
Sbjct: 153 IHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHG 212

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 255
           THTA TAAG FVKGAEALGNA G AVGMAPLAH+AMYKVCS  GCSSSD+LA LDAA+DD
Sbjct: 213 THTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDD 272

Query: 256 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVVR 315
           GVDVLSLSLG+ S PFF+D +A+G FAAI+K                       ++L V 
Sbjct: 273 GVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVG 332

Query: 316 PE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTDI 375
                                     QP +F S FLPLVYAG +G + S +C QGSL  +
Sbjct: 333 ASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKL 392

Query: 376 DVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYEA 435
           +V GKVVVC+  GG+ R+AKG VVKN GGAAMILINQ+ +GF+T +EAHVLP +H+SYE 
Sbjct: 393 NVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYED 452

Query: 436 GLKIKAYISSSQNPTASISFKGTIIGDGSS--SPAMASFSSRGPSLSSPGILKPDITGPG 495
           GLKIKAYI+SS NP ASISFKGT++G+ ++  SPAMASFSSRGP  +SPGILKPDITGPG
Sbjct: 453 GLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPG 512

Query: 496 VNILAAWPFPLDN--NTDTRSTFNVISGTSMSCPHLSGIAALIKSY-------------- 555
           VNILAAWPFPL+N  NT+T+STFNVISGTSMSCPHLSGIAALIKSY              
Sbjct: 513 VNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIM 572

Query: 556 -----PNPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKD 615
                 NP+GKPI+DQDLKPAN FA+G+GHV+PS+AA+PGLVYDI+PDDYIPYLC LY D
Sbjct: 573 TSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTD 632

Query: 616 AEVSIIVQKPVICSLVPRIREGDLNYPSFSVALGP-LQTFKRTVTNVGDANSVYSAIIEA 618
           A+VSIIV+K V CS VPRIREGDLNYPSF+V+LG   QTF RTVTNVGDANSVY  I+EA
Sbjct: 633 AQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEA 692

BLAST of Sgr024350 vs. NCBI nr
Match: KAA0042822.1 (subtilisin-like protease SBT1.7 [Cucumis melo var. makuwa])

HSP 1 Score: 863.2 bits (2229), Expect = 1.4e-246
Identity = 455/672 (67.71%), Postives = 520/672 (77.38%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTE-NSIEQPRLLYSYRNVMSGFSARL 75
           LQTYIVHVKQP + +LG +T D+++WY SFLPET E +S EQPRLLYSYR+VMSGFSARL
Sbjct: 13  LQTYIVHVKQPVLEILG-DTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARL 72

Query: 76  TEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTG 135
           T+EQV+AME KDGFISA PET L+LHTTHTP +LGLN  FGLWK+SNFG+GVIIGVLDTG
Sbjct: 73  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTG 132

Query: 136 ITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
           I P HPSF DEGMS PPAKWKGRCEF +S+CNNKLIGARTFN AN    G+SP DENGHG
Sbjct: 133 IHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHG 192

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 255
           THTA TAAG FVKGAEALGNA G AVGMAPLAH+AMYKVCS  GCSSSD+LA LDAA+DD
Sbjct: 193 THTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDD 252

Query: 256 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVVR 315
           GVDVLSLSLG+ S PFF+D +AIG FAAI+K                       ++L V 
Sbjct: 253 GVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVG 312

Query: 316 PE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTDI 375
                                     QP +F S FLPLVYAG +G + S +C QGSL  +
Sbjct: 313 ASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKL 372

Query: 376 DVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYEA 435
           +V GKVVVC+  GG+ R+AKG VVKN GGAAMILINQ+ +GF+T +EAHVLP +H+SYE 
Sbjct: 373 NVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYED 432

Query: 436 GLKIKAYISSSQNPTASISFKGTIIGDGSS--SPAMASFSSRGPSLSSPGILKPDITGPG 495
           GLKIKAYI+SS NP ASISFKGT++G+ ++  SPAMASFSSRGP  +SPGILKPDITGPG
Sbjct: 433 GLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPG 492

Query: 496 VNILAAWPFPLDN--NTDTRSTFNVISGTSMSCPHLSGIAALIKSY-------------- 555
           VNILAAWPFPL+N  NT+T+STFNVISGTSMSCPHLSGIAALIKSY              
Sbjct: 493 VNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIM 552

Query: 556 -----PNPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKD 615
                 NP+GKPI+DQDLKPAN FA+G+GHV+PS+AA+PGLVYDI+PDDYIPYLC LY D
Sbjct: 553 TSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTD 612

Query: 616 AEVSIIVQKPVICSLVPRIREGDLNYPSFSVAL-GPLQTFKRTVTNVGDANSVYSAIIEA 618
           A+VSIIV+K V CS VPRIREGDLNYPSF+V+L    QTF RTVTNVGDANSVY  I+EA
Sbjct: 613 AQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEA 672

BLAST of Sgr024350 vs. ExPASy Swiss-Prot
Match: A9QY38 (Subtilisin-like protease 4 OS=Lotus japonicus OX=34305 GN=SBTM4 PE=2 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 2.3e-204
Identity = 384/667 (57.57%), Postives = 464/667 (69.57%), Query Frame = 0

Query: 19  YIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLTEEQ 78
           YI+HV  PE ++L  E+ D+ESWY SFLP T  +S EQPR++YSY+NV+ GF+A LT+E+
Sbjct: 41  YIIHVTGPEGKML-TESEDLESWYHSFLPPTLMSSEEQPRVIYSYKNVLRGFAASLTQEE 100

Query: 79  VEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTGITPT 138
           + A+E K+GFISA P+ +L   TTHTP FLGL Q  G+WK+SNFG+GVIIGVLD+GITP 
Sbjct: 101 LSAVEKKNGFISAHPQRVLHRQTTHTPKFLGLQQDTGVWKESNFGKGVIIGVLDSGITPG 160

Query: 139 HPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRA----NGLWKGESPADENGHG 198
           HPSF D G+ PPP KWKGRC+ N + CNNKLIGAR FN A    NG  K E+P DE+GHG
Sbjct: 161 HPSFSDVGIPPPPPKWKGRCDLNVTACNNKLIGARAFNLAAEAMNGK-KAEAPIDEDGHG 220

Query: 199 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 258
           THTA TAAGAFV  AE LGNA+GTA GMAP AHLA+YKVC  + C  SD+LA LDAAV+D
Sbjct: 221 THTASTAAGAFVNYAEVLGNAKGTAAGMAPHAHLAIYKVCFGEDCPESDILAALDAAVED 280

Query: 259 GVDVLSLSLG-SRSAPFFKDNVAIGTFAAIQK----------------------EFLLVV 318
           GVDV+S+SLG S   PFF D+ AIG FAA+QK                       ++L V
Sbjct: 281 GVDVISISLGLSEPPPFFNDSTAIGAFAAMQKGIFVSCAAGNSGPFNSSIVNAAPWILTV 340

Query: 319 -----------------------RPEIQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTD 378
                                      QPS F  T LPL YAG NG + SAFC  GSL D
Sbjct: 341 GASTIDRRIVATAKLGNGQEFDGESVFQPSSFTPTLLPLAYAGKNGKEESAFCANGSLDD 400

Query: 379 IDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYE 438
              +GKVV+C+  GG+ RIAKG  VK AGGAAMIL+N + + F+  ++ H LP +HVSY 
Sbjct: 401 SAFRGKVVLCERGGGIARIAKGEEVKRAGGAAMILMNDETNAFSLSADVHALPATHVSYA 460

Query: 439 AGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSPGILKPDITGPGV 498
           AG++IKAYI+S+  PTA+I FKGT+IG+ S +PA+ASFSSRGP+L SPGILKPDI GPGV
Sbjct: 461 AGIEIKAYINSTATPTATILFKGTVIGN-SLAPAVASFSSRGPNLPSPGILKPDIIGPGV 520

Query: 499 NILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIK-SYP--------------- 558
           NILAAWPFPL N+TD++ TFN+ SGTSMSCPHLSGIAAL+K S+P               
Sbjct: 521 NILAAWPFPLSNSTDSKLTFNIESGTSMSCPHLSGIAALLKSSHPHWSPAAIKSAIMTSA 580

Query: 559 ---NPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGL-YKDAE 616
              N   K IVD+ L+P +LFA G+GHV+PSRA DPGLVYDI+PDDYIPYLCGL Y + E
Sbjct: 581 DTINLGNKLIVDETLQPTDLFATGSGHVNPSRANDPGLVYDIQPDDYIPYLCGLGYSETE 640

BLAST of Sgr024350 vs. ExPASy Swiss-Prot
Match: A0A0M3R8G2 (Subtilisin-like protease OS=Petunia hybrida OX=4102 GN=SBT PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.6e-189
Identity = 366/680 (53.82%), Postives = 453/680 (66.62%), Query Frame = 0

Query: 6   LSFQLL---RPKQLQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYS 65
           LSF L    R   LQTYIVHV +P+ ++L     D+ES+Y SFLP T   S    R+++S
Sbjct: 13  LSFHLTTAQRSTGLQTYIVHVDKPDAQVLANSA-DLESYYNSFLPATVSGSEVPSRIIHS 72

Query: 66  YRNVMSGFSARLTEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNF 125
           Y +V +GF+A+L+ E+V+ ME K GF+SA  E +L+LHTTHTP FLGL Q  G W++SN+
Sbjct: 73  YHHVATGFAAKLSGEEVKEMEKKAGFVSAKLEKVLTLHTTHTPNFLGLYQNMGFWQESNY 132

Query: 126 GEGVIIGVLDTGITPTHPSFGDEGMSPPPAKWKGRCEF-NSSVCNNKLIGARTFNRANGL 185
           G+GVIIG+LDTGITP HPSF D  M  PPAKWKG+CEF  ++ CN K+IGAR F   +G+
Sbjct: 133 GKGVIIGLLDTGITPGHPSFSDVNMPSPPAKWKGKCEFTGNATCNKKIIGARNFISGSGV 192

Query: 186 WKGESPADENGHGTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSS 245
                P DE GHGTHTA TAAG FV  A   GNA GTAVGMAPLAH+AMYKVCS  GCS 
Sbjct: 193 ----PPTDEEGHGTHTASTAAGNFVNDANVFGNANGTAVGMAPLAHIAMYKVCSEDGCSD 252

Query: 246 SDVLAGLDAAVDDGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK---------------- 305
           +D+LA LDAA+DDGVDVLSLSLG  S PF+ DN+AIG FAAI+K                
Sbjct: 253 ADILAALDAAIDDGVDVLSLSLGGYSDPFYYDNIAIGAFAAIRKGIFVSASAGNDGPLNS 312

Query: 306 ------EFLLVV-----------------------RPEIQPSDFLSTFLPLVYAGSNGNQ 365
                  ++L V                           QP+DF  T LPLVY G++ ++
Sbjct: 313 TLSNEAPWILTVGASTHDRKIVATAVLGNGQQYDGESAFQPADFPHTLLPLVYPGTS-DE 372

Query: 366 TSAFCGQGSLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSE 425
            +AFC  GSL   DVKGKVVVC   G V R+ K   VK+AGGAAMIL N + DG  TF++
Sbjct: 373 EAAFCSSGSLDKFDVKGKVVVCDRGGDVARLEKSQTVKDAGGAAMILANLEIDGEGTFAD 432

Query: 426 AHVLPVSHVSYEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSP 485
           AHVLP +HV Y AG  IK+YI+S+  PTA I FKGTIIG   SSP+++SFSSRGP+L+SP
Sbjct: 433 AHVLPATHVGYAAGEMIKSYINSTSTPTAGILFKGTIIG-FKSSPSVSSFSSRGPNLASP 492

Query: 486 GILKPDITGPGVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS------ 545
           GI+KPDI GPGVNILAAWP  ++N T T  TFN+ISGTSMSCPHLSGI AL+KS      
Sbjct: 493 GIVKPDIIGPGVNILAAWPVSVENKTGTDLTFNIISGTSMSCPHLSGIVALLKSAHPDWS 552

Query: 546 -------------YPNPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYI 605
                          N EG+PI+D+   PA++FA GAGHV+PS+A+DPGL+YDI+ +DYI
Sbjct: 553 PAAIKSAIMTSADQSNLEGQPILDERNLPADIFATGAGHVNPSKASDPGLIYDIQLEDYI 612

Query: 606 PYLCGL-YKDAEVSIIVQKPVICSLVPRIREGDLNYPSFSVALGP-LQTFKRTVTNVGDA 616
            YLCGL Y++ ++ +IVQ+ V C     I E +LNYPSFS+ LGP  Q + RTVTNVGDA
Sbjct: 613 QYLCGLGYREKDIGLIVQETVKCE--SSISEAELNYPSFSIILGPKTQNYTRTVTNVGDA 672

BLAST of Sgr024350 vs. ExPASy Swiss-Prot
Match: A9QY39 (Subtilisin-like protease 3 OS=Lotus japonicus OX=34305 GN=SBTM3 PE=2 SV=1)

HSP 1 Score: 647.5 bits (1669), Expect = 1.5e-184
Identity = 357/668 (53.44%), Postives = 443/668 (66.32%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLT 75
           L TYIVHV++P++     +++D+ ++Y S LPE+T+ +    R++++YRNV++GF+ +LT
Sbjct: 42  LSTYIVHVRKPQV----IQSDDLHTFYYSLLPESTKTT--NQRIVFTYRNVVNGFAVKLT 101

Query: 76  EEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTGI 135
            E+ +A++  +  +SA PE ILSLHTTHTP+FLGL Q  GLWK SN G+GVIIG+LDTGI
Sbjct: 102 PEEAKALQQNEEVVSARPEKILSLHTTHTPSFLGLQQGLGLWKGSNSGKGVIIGILDTGI 161

Query: 136 TPTHPSFGDEGMSPPPAKWKGRCEFNSS-VCNNKLIGARTFNRANGLWKGESPADENGHG 195
           +P HPSF DEGM  PPAKW G CEF     CNNK+IGAR F +   L     P D+ GHG
Sbjct: 162 SPFHPSFSDEGMPSPPAKWNGICEFTGKRTCNNKIIGARNFVKTKNL---TLPFDDVGHG 221

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 255
           THTA TAAG  V+GA   GNA GTAVGMAP AH+AMYKVC   GCS S +LAG+D AVDD
Sbjct: 222 THTASTAAGRPVQGANVYGNANGTAVGMAPDAHIAMYKVCGLVGCSESAILAGMDTAVDD 281

Query: 256 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQKEFLL------------------------- 315
           GVDVLSLSLG  S PFF+D +A+G F AIQK   +                         
Sbjct: 282 GVDVLSLSLGGPSGPFFEDPIALGAFGAIQKGIFVSCSAANSGPAYSSLSNEAPWILTVG 341

Query: 316 --------------------VVRPEIQPSDFLSTFLPLVYAGSNGNQT-SAFCGQGSLTD 375
                               V +   QP DF  + LPLVYAG+NGN   S FC   SL  
Sbjct: 342 ASSIDRTIMATAKLGNGKEYVGQSVFQPKDFAPSLLPLVYAGANGNNNFSVFCAPESLNR 401

Query: 376 IDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYE 435
            DV+GKVV+C+  G V R+ KG  VK+AGGAAMIL+N   + F   ++ HVLP  H+SYE
Sbjct: 402 SDVEGKVVLCEDGGFVPRVFKGKAVKDAGGAAMILMNSVLEDFNPIADVHVLPAVHISYE 461

Query: 436 AGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSPGILKPDITGPGV 495
           AGL +K YI+S+  PTA+I F+GT+IG+   +P + SFSSRGPS +SPGILKPDI GPG+
Sbjct: 462 AGLALKEYINSTSTPTATILFEGTVIGN-LLAPQVTSFSSRGPSKASPGILKPDIIGPGL 521

Query: 496 NILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIK-SYP--------------- 555
           NILAAWP  LDN+  T   FN+ISGTSMSCPHLSGIAAL+K S+P               
Sbjct: 522 NILAAWPVSLDNS--TTPPFNIISGTSMSCPHLSGIAALLKNSHPDWSPAAIKSAIMTTA 581

Query: 556 ---NPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGL-YKDAE 615
              N  G PI+DQ L PA++FA GAGHV+P +A DPGLVYDI P+DYIPYLCGL Y D E
Sbjct: 582 SQVNLGGTPILDQRLVPADVFATGAGHVNPVKANDPGLVYDIEPNDYIPYLCGLNYTDRE 641

BLAST of Sgr024350 vs. ExPASy Swiss-Prot
Match: A9QY40 (Subtilisin-like protease 1 OS=Lotus japonicus OX=34305 GN=SBTM1 PE=2 SV=1)

HSP 1 Score: 641.7 bits (1654), Expect = 8.4e-183
Identity = 365/670 (54.48%), Postives = 441/670 (65.82%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLT 75
           L TYIVHVK+ EI    + T ++ +W+ SFLPET+     + R+++SYRNV SGF+ RLT
Sbjct: 38  LTTYIVHVKKLEIEGPLQSTEELHTWHHSFLPETS----NKDRMVFSYRNVASGFAVRLT 97

Query: 76  EEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTGI 135
            E+  A++ K+  +S  PE  LSLHTTHTP+FLGL Q  GLW DSN G+GVIIGV+DTGI
Sbjct: 98  PEEANALQEKEEVMSIRPERTLSLHTTHTPSFLGLRQGQGLWNDSNLGKGVIIGVIDTGI 157

Query: 136 TPTHPSFGDEGMSPPPAKWKGRCEF-NSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
            P H SF DEGM PPPAKWKG CEF   SVCNNKLIGAR   ++      E P ++  HG
Sbjct: 158 YPFHLSFNDEGMPPPPAKWKGHCEFTGGSVCNNKLIGARNLVKSA---IQEPPYEDFFHG 217

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSK---GCSSSDVLAGLDAA 255
           THTA  AAG FV+GA   GNA GTA GMAP AHLA+YKVCSSK    C  S +LA +D A
Sbjct: 218 THTAAEAAGRFVEGASVFGNARGTAAGMAPDAHLAIYKVCSSKVKDECPESAILAAMDIA 277

Query: 256 VDDGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLL 315
           ++DGVDVLSLSLG  S PFF+D +AIG FAA QK                       ++L
Sbjct: 278 IEDGVDVLSLSLGLGSLPFFEDPIAIGAFAATQKGIFVSCSAANSGPHYSSLSNEAPWIL 337

Query: 316 VVRPE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSL 375
            V                           QP DF S  LPLVYA +  N +SA C  GSL
Sbjct: 338 TVGASTIDRKISASAKLGNGAEYEGETLFQPKDFSSQLLPLVYAAAEKNNSSALCAPGSL 397

Query: 376 TDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVS 435
            +I+VKGKVVVC   GG+  IAKG  V +AGG+AMIL N +  GFTT + AHVLP  HVS
Sbjct: 398 RNINVKGKVVVCDLGGGIPFIAKGQEVLDAGGSAMILANIENFGFTTLANAHVLPAVHVS 457

Query: 436 YEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSPGILKPDITGP 495
           Y A L IKAYI+S+  PTA++ F+GTIIGD S +P++A+FSSRGPS  SPGILKPDI GP
Sbjct: 458 YAASLAIKAYINSTYTPTATVLFQGTIIGD-SLAPSVAAFSSRGPSQQSPGILKPDIIGP 517

Query: 496 GVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS-YP------------- 555
           GVNILAAW   +DN       F++ISGTSMSCPHLSGIAAL+KS +P             
Sbjct: 518 GVNILAAWAVSVDNKI---PAFDIISGTSMSCPHLSGIAALLKSAHPDWSPAAIKSAIMT 577

Query: 556 -----NPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGL-YKD 615
                N  G PI+DQ L+PA++FA GAGHV+P RA DPGLVYDI+P+DY+PYLCGL Y D
Sbjct: 578 TANTLNLRGLPILDQRLQPADIFATGAGHVNPVRANDPGLVYDIQPEDYVPYLCGLGYSD 637

BLAST of Sgr024350 vs. ExPASy Swiss-Prot
Match: G7KEU7 (Subtilisin-like protease OS=Medicago truncatula OX=3880 GN=SBT PE=2 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 6.0e-173
Identity = 349/672 (51.93%), Postives = 424/672 (63.10%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLT 75
           L TYIVHVK+ E  +   ++ D+ SWY SFLP+T  +   + R+++SYR V SGF+ +LT
Sbjct: 35  LMTYIVHVKKSE-NVASHQSEDLHSWYHSFLPQTFPH---KERMVFSYRKVASGFAVKLT 94

Query: 76  EEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTGI 135
            E+ ++++ K   +SA PE  L LHTTHTP FLGL Q  GLW D N G+GVIIG++DTGI
Sbjct: 95  PEEAKSLQEKGEIVSARPERTLELHTTHTPTFLGLKQGQGLWSDDNLGKGVIIGIIDTGI 154

Query: 136 TPTHPSFGDEGMSPPPAKWKGRCEF-NSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
            P HPSF DEGM PPPAKWKG CEF    VCNNKLIGAR   ++      E P +   HG
Sbjct: 155 FPLHPSFNDEGMPPPPAKWKGHCEFTGGQVCNNKLIGARNLVKSA---IQEPPFENFFHG 214

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSK-GCSSSDVLAGLDAAVD 255
           THTA  AAG F++ A   GNA+G A GMAP AHLA+YKVC+ K GC+ S +LA +D A++
Sbjct: 215 THTAAEAAGRFIEDASVFGNAKGVAAGMAPNAHLAIYKVCNDKIGCTESAILAAMDIAIE 274

Query: 256 DGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVV 315
           DGVDVLSLSLG  S PFF+D +AIG FAA Q                        ++L V
Sbjct: 275 DGVDVLSLSLGLGSLPFFEDPIAIGAFAATQNGVFVSCSAANSGPGYSTLSNEAPWILTV 334

Query: 316 RPE-----------------------IQPSDFLSTFLPLVYAGS----NGNQTSAFCGQG 375
                                      QP DF    LPLVY GS    N  Q  + C  G
Sbjct: 335 GASTIDRKIVASAKLGNGEEYEGETLFQPKDFSQQLLPLVYPGSFGYGNQTQNQSLCLPG 394

Query: 376 SLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSH 435
           SL +ID+ GKVV+C   G V  I KG  V N+GG AMIL N +  GF+TF+ AHVLP   
Sbjct: 395 SLKNIDLSGKVVLCD-VGNVSSIVKGQEVLNSGGIAMILANSEALGFSTFAIAHVLPAVE 454

Query: 436 VSYEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSPGILKPDIT 495
           VSY AGL IK+YI S+ NPTA++ FKGTIIGD S +P++  FSSRGPS  SPGILKPDI 
Sbjct: 455 VSYAAGLTIKSYIKSTYNPTATLIFKGTIIGD-SLAPSVVYFSSRGPSQESPGILKPDII 514

Query: 496 GPGVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIK-SYP----------- 555
           GPGVNILAAW   +DN       F+++SGTSMSCPHLSGIAALIK S+P           
Sbjct: 515 GPGVNILAAWAVSVDNKI---PAFDIVSGTSMSCPHLSGIAALIKSSHPDWSPAAIKSAI 574

Query: 556 -------NPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGL-Y 615
                  N  G PI+DQ L PA++FA GAGHV+P +A DPGLVYDI P+DY+PYLCGL Y
Sbjct: 575 MTTANTLNLGGIPILDQRLFPADIFATGAGHVNPVKANDPGLVYDIEPEDYVPYLCGLGY 634

BLAST of Sgr024350 vs. ExPASy TrEMBL
Match: A0A5A7TMM4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003280 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 7.9e-269
Identity = 479/669 (71.60%), Postives = 546/669 (81.61%), Query Frame = 0

Query: 15  QLQTYIVHVKQPEIRLLGEETND-IESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSAR 74
           +LQTYIVHV+QP+IRL+GE +ND IESWYMSF+P++TE ++EQP+LLYSYRNVMSGFSAR
Sbjct: 33  KLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSAR 92

Query: 75  LTEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDT 134
           LT EQV+AME KDGFISA PETI+SLHTTHTP +LGLNQQFG+WK+SNFG+GVI+GVLDT
Sbjct: 93  LTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDT 152

Query: 135 GITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGH 194
           GI P HPSF DEGMSPPPAKWKG+CEFNSS+CNNKLIGARTFN  N     ESP DE GH
Sbjct: 153 GIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGH 212

Query: 195 GTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVD 254
           GTHTA TAAGAFV+ AEALGNA+G A G+APLAHLA+YKVCS K C SSDV AG+DAA+D
Sbjct: 213 GTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAID 272

Query: 255 DGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVV 314
           DGVDVLS+SLGSRS PFFKDN+A+ TF AIQK                       ++L V
Sbjct: 273 DGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTV 332

Query: 315 RPE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTD 374
                                      QP+DF S FLPLVYAG+  N+T AFCG+GSL +
Sbjct: 333 GASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLEN 392

Query: 375 IDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYE 434
           +DVKGKVVVC+G+GGVGR+AKG VVKNAGGAAMILINQ+EDGF+T SEAHVLP +HVSY+
Sbjct: 393 MDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYK 452

Query: 435 AGLKIKAYISSSQNPTASISFKGTIIGDGS--SSPAMASFSSRGPSLSSPGILKPDITGP 494
           AG+ IK+YI+SSQNPTASISFKGT+IGDG   S+P+MASFSSRGP L SPGILKPDITGP
Sbjct: 453 AGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGP 512

Query: 495 GVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS-YPN------------ 554
           GVNILAAWPFPLDN+T+T+STFNVISGTSMSCPHLSGIAALIKS +PN            
Sbjct: 513 GVNILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 572

Query: 555 ------PEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKDA 614
                 P+G+PIVDQDL+PAN FA+GAGHV+PS+AADPGLVYDI+PDDYIPYLCGLYKD 
Sbjct: 573 TANIKTPQGEPIVDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 632

Query: 615 EVSIIVQKPVICSLVPRIREGDLNYPSFSVALGPLQTFKRTVTNVGDANSVYSAIIEAPL 617
           EVSIIV + VIC LVP IREGDLNYPSFSVALG LQTFKRTVTNVG+ANSVY+AI+EAPL
Sbjct: 633 EVSIIVHRTVICGLVPSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPL 692

BLAST of Sgr024350 vs. ExPASy TrEMBL
Match: A0A1S3AUP1 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103483066 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.3e-267
Identity = 477/669 (71.30%), Postives = 544/669 (81.32%), Query Frame = 0

Query: 15  QLQTYIVHVKQPEIRLLGEETND-IESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSAR 74
           +LQTYIVHV+QP+IRL+GE +ND IESWYMSF+P++TE ++EQP+LLYSYRNVMSGFSAR
Sbjct: 42  KLQTYIVHVEQPKIRLVGESSNDHIESWYMSFIPKSTETTVEQPQLLYSYRNVMSGFSAR 101

Query: 75  LTEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDT 134
           LT EQV+AME KDGFISA PETI+SLHTTHTP +LGLNQQFG+WK+SNFG+GVI+GVLDT
Sbjct: 102 LTIEQVKAMEKKDGFISAMPETIMSLHTTHTPEYLGLNQQFGIWKNSNFGKGVIVGVLDT 161

Query: 135 GITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGH 194
           GI P HPSF DEGMSPPPAKWKG+CEFNSS+CNNKLIGARTFN  N     ESP DE GH
Sbjct: 162 GIHPNHPSFNDEGMSPPPAKWKGKCEFNSSLCNNKLIGARTFNLGNNFLMEESPNDEKGH 221

Query: 195 GTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVD 254
           GTHTA TAAGAFV+ AEALGNA+G A G+APLAHLA+YKVCS K C SSDV AG+DAA+D
Sbjct: 222 GTHTASTAAGAFVEDAEALGNAKGKAAGIAPLAHLAIYKVCSGKRCPSSDVFAGIDAAID 281

Query: 255 DGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVV 314
           DGVDVLS+SLGSRS PFFKDN+A+ TF AIQK                       ++L V
Sbjct: 282 DGVDVLSISLGSRSVPFFKDNIAVATFGAIQKGIFVSSSAGNSGPLNSTLSNDAPWILTV 341

Query: 315 RPE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTD 374
                                      QP+DF S FLPLVYAG+  N+T AFCG+GSL +
Sbjct: 342 GASTINRRIVAVAKLGNGEQYEGESLYQPNDFPSKFLPLVYAGNRENKTYAFCGEGSLEN 401

Query: 375 IDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYE 434
           +DVKGKVVVC+G+GGVGR+AKG VVKNAGGAAMILINQ+EDGF+T SEAHVLP +HVSY+
Sbjct: 402 MDVKGKVVVCEGKGGVGRVAKGLVVKNAGGAAMILINQEEDGFSTLSEAHVLPATHVSYK 461

Query: 435 AGLKIKAYISSSQNPTASISFKGTIIGDGS--SSPAMASFSSRGPSLSSPGILKPDITGP 494
           AG+ IK+YI+SSQNPTASISFKGT+IGDG   S+P+MASFSSRGP L SPGILKPDITGP
Sbjct: 462 AGVLIKSYINSSQNPTASISFKGTVIGDGDDFSAPSMASFSSRGPCLPSPGILKPDITGP 521

Query: 495 GVNILAAWPFPLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS-YPN------------ 554
           GVNILAAWPFPLDN+T+T+STFNVISGTSMSCPHLSGIAALIKS +PN            
Sbjct: 522 GVNILAAWPFPLDNDTNTKSTFNVISGTSMSCPHLSGIAALIKSVHPNWSPAAIKSAIMT 581

Query: 555 ------PEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKDA 614
                 P+G+PI DQDL+PAN FA+GAGHV+PS+AADPGLVYDI+PDDYIPYLCGLYKD 
Sbjct: 582 TANIKTPQGEPITDQDLQPANFFAMGAGHVNPSKAADPGLVYDIQPDDYIPYLCGLYKDE 641

Query: 615 EVSIIVQKPVICSLVPRIREGDLNYPSFSVALGPLQTFKRTVTNVGDANSVYSAIIEAPL 617
           EVSIIV + VIC LV  IREGDLNYPSFSVALG LQTFKRTVTNVG+ANSVY+AI+EAPL
Sbjct: 642 EVSIIVHRTVICGLVLSIREGDLNYPSFSVALGGLQTFKRTVTNVGEANSVYTAIVEAPL 701

BLAST of Sgr024350 vs. ExPASy TrEMBL
Match: A0A1S3AT16 (subtilisin-like protease SBT1.7 OS=Cucumis melo OX=3656 GN=LOC103482683 PE=3 SV=1)

HSP 1 Score: 865.5 bits (2235), Expect = 1.3e-247
Identity = 455/672 (67.71%), Postives = 522/672 (77.68%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTE-NSIEQPRLLYSYRNVMSGFSARL 75
           LQTYIVHVKQP + +LG +T D+++WY SFLPET E +S EQPRLLYSYR+VMSGFSARL
Sbjct: 33  LQTYIVHVKQPVLEILG-DTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARL 92

Query: 76  TEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTG 135
           T+EQV+AME KDGFISA PET L+LHTTHTP +LGLN+ FGLWK+SNFG+GVIIGVLDTG
Sbjct: 93  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNKHFGLWKNSNFGKGVIIGVLDTG 152

Query: 136 ITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
           I P HPSF DEGMS PPAKWKGRCEF +S+CNNKLIGARTFN AN    G+SP DENGHG
Sbjct: 153 IHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHG 212

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 255
           THTA TAAG FVKGAEALGNA G AVGMAPLAH+AMYKVCS  GCSSSD+LA LDAA+DD
Sbjct: 213 THTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDD 272

Query: 256 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVVR 315
           GVDVLSLSLG+ S PFF+D +A+G FAAI+K                       ++L V 
Sbjct: 273 GVDVLSLSLGAPSTPFFRDTIAMGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVG 332

Query: 316 PE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTDI 375
                                     QP +F S FLPLVYAG +G + S +C QGSL  +
Sbjct: 333 ASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKL 392

Query: 376 DVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYEA 435
           +V GKVVVC+  GG+ R+AKG VVKN GGAAMILINQ+ +GF+T +EAHVLP +H+SYE 
Sbjct: 393 NVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYED 452

Query: 436 GLKIKAYISSSQNPTASISFKGTIIGDGSS--SPAMASFSSRGPSLSSPGILKPDITGPG 495
           GLKIKAYI+SS NP ASISFKGT++G+ ++  SPAMASFSSRGP  +SPGILKPDITGPG
Sbjct: 453 GLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPG 512

Query: 496 VNILAAWPFPLDN--NTDTRSTFNVISGTSMSCPHLSGIAALIKSY-------------- 555
           VNILAAWPFPL+N  NT+T+STFNVISGTSMSCPHLSGIAALIKSY              
Sbjct: 513 VNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIM 572

Query: 556 -----PNPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKD 615
                 NP+GKPI+DQDLKPAN FA+G+GHV+PS+AA+PGLVYDI+PDDYIPYLC LY D
Sbjct: 573 TSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTD 632

Query: 616 AEVSIIVQKPVICSLVPRIREGDLNYPSFSVALGP-LQTFKRTVTNVGDANSVYSAIIEA 618
           A+VSIIV+K V CS VPRIREGDLNYPSF+V+LG   QTF RTVTNVGDANSVY  I+EA
Sbjct: 633 AQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLGAGSQTFNRTVTNVGDANSVYYPIVEA 692

BLAST of Sgr024350 vs. ExPASy TrEMBL
Match: A0A5A7THE4 (Subtilisin-like protease SBT1.7 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G003290 PE=3 SV=1)

HSP 1 Score: 863.2 bits (2229), Expect = 6.5e-247
Identity = 455/672 (67.71%), Postives = 520/672 (77.38%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTE-NSIEQPRLLYSYRNVMSGFSARL 75
           LQTYIVHVKQP + +LG +T D+++WY SFLPET E +S EQPRLLYSYR+VMSGFSARL
Sbjct: 13  LQTYIVHVKQPVLEILG-DTIDLQNWYTSFLPETIEASSDEQPRLLYSYRHVMSGFSARL 72

Query: 76  TEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTG 135
           T+EQV+AME KDGFISA PET L+LHTTHTP +LGLN  FGLWK+SNFG+GVIIGVLDTG
Sbjct: 73  TKEQVKAMEEKDGFISAMPETTLNLHTTHTPEYLGLNTHFGLWKNSNFGKGVIIGVLDTG 132

Query: 136 ITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
           I P HPSF DEGMS PPAKWKGRCEF +S+CNNKLIGARTFN AN    G+SP DENGHG
Sbjct: 133 IHPNHPSFNDEGMSSPPAKWKGRCEFGASICNNKLIGARTFNLANNFVIGKSPNDENGHG 192

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 255
           THTA TAAG FVKGAEALGNA G AVGMAPLAH+AMYKVCS  GCSSSD+LA LDAA+DD
Sbjct: 193 THTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAMYKVCSPNGCSSSDILAALDAAIDD 252

Query: 256 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVVR 315
           GVDVLSLSLG+ S PFF+D +AIG FAAI+K                       ++L V 
Sbjct: 253 GVDVLSLSLGAPSTPFFRDTIAIGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVG 312

Query: 316 PE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTDI 375
                                     QP +F S FLPLVYAG +G + S +C QGSL  +
Sbjct: 313 ASTIDRKIVALAKLGSGEVYTGESLFQPRNFPSKFLPLVYAGKSGIEGSEYCIQGSLEKL 372

Query: 376 DVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYEA 435
           +V GKVVVC+  GG+ R+AKG VVKN GGAAMILINQ+ +GF+T +EAHVLP +H+SYE 
Sbjct: 373 NVTGKVVVCERGGGISRVAKGLVVKNGGGAAMILINQKPEGFSTLAEAHVLPTTHLSYED 432

Query: 436 GLKIKAYISSSQNPTASISFKGTIIGDGSS--SPAMASFSSRGPSLSSPGILKPDITGPG 495
           GLKIKAYI+SS NP ASISFKGT++G+ ++  SPAMASFSSRGP  +SPGILKPDITGPG
Sbjct: 433 GLKIKAYINSSHNPKASISFKGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPG 492

Query: 496 VNILAAWPFPLDN--NTDTRSTFNVISGTSMSCPHLSGIAALIKSY-------------- 555
           VNILAAWPFPL+N  NT+T+STFNVISGTSMSCPHLSGIAALIKSY              
Sbjct: 493 VNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSYHPNWSPAAIKSAIM 552

Query: 556 -----PNPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKD 615
                 NP+GKPI+DQDLKPAN FA+G+GHV+PS+AA+PGLVYDI+PDDYIPYLC LY D
Sbjct: 553 TSADVRNPQGKPIMDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYIPYLCHLYTD 612

Query: 616 AEVSIIVQKPVICSLVPRIREGDLNYPSFSVAL-GPLQTFKRTVTNVGDANSVYSAIIEA 618
           A+VSIIV+K V CS VPRIREGDLNYPSF+V+L    QTF RTVTNVGDANSVY  I+EA
Sbjct: 613 AQVSIIVRKQVTCSTVPRIREGDLNYPSFAVSLEAGSQTFNRTVTNVGDANSVYYPIVEA 672

BLAST of Sgr024350 vs. ExPASy TrEMBL
Match: A0A0A0KMQ7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G157280 PE=3 SV=1)

HSP 1 Score: 860.5 bits (2222), Expect = 4.2e-246
Identity = 451/672 (67.11%), Postives = 523/672 (77.83%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTE-NSIEQPRLLYSYRNVMSGFSARL 75
           LQTYIVHVKQPE+ +LG +T D+++WY SFLPET E +S EQ RLLYSYR+V+SGFSARL
Sbjct: 13  LQTYIVHVKQPEVEILG-DTIDLQNWYTSFLPETIEASSNEQSRLLYSYRHVISGFSARL 72

Query: 76  TEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTG 135
           T+EQV+ ME KDGFISA PET L+LHTTHTP +LGLNQ FGLWK+SNFG+GVIIGVLDTG
Sbjct: 73  TKEQVKTMEEKDGFISAMPETTLNLHTTHTPEYLGLNQHFGLWKNSNFGKGVIIGVLDTG 132

Query: 136 ITPTHPSFGDEGMSPPPAKWKGRCEFNSSVCNNKLIGARTFNRANGLWKGESPADENGHG 195
           I P HPSF DEGM  PPAKWKGRCEF +S+CNNKLIGARTFN AN +  G+SP DENGHG
Sbjct: 133 IHPNHPSFNDEGMPSPPAKWKGRCEFGASICNNKLIGARTFNLANNVSIGKSPNDENGHG 192

Query: 196 THTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSDVLAGLDAAVDD 255
           THTA TAAG FVKGAEALGNA G AVGMAPLAH+A+YKVCS KGCSSSD+LA LDAA+DD
Sbjct: 193 THTASTAAGTFVKGAEALGNARGKAVGMAPLAHIAVYKVCSPKGCSSSDILAALDAAIDD 252

Query: 256 GVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK----------------------EFLLVVR 315
           GVDVLSLSLG+ S PFFKD +A+G FAAI+K                       ++L V 
Sbjct: 253 GVDVLSLSLGAPSTPFFKDTIAVGAFAAIKKGIFVSCSAGNSGPSKNTLANEAPWILTVG 312

Query: 316 PE-----------------------IQPSDFLSTFLPLVYAGSNGNQTSAFCGQGSLTDI 375
                                     QP DF S FLPLVYAG +G + S +C +GSL  +
Sbjct: 313 ASTIDRKIVALAKLESGKVFTGESLFQPRDFSSKFLPLVYAGKSGIEGSEYCVEGSLEKL 372

Query: 376 DVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHVLPVSHVSYEA 435
           +V GK+VVC+  GG+GRIAKG VVKN GGAAMIL+NQ+ DGF+T +EAHVLP +H+SYE 
Sbjct: 373 NVTGKIVVCERGGGIGRIAKGLVVKNGGGAAMILVNQKPDGFSTLAEAHVLPTTHLSYED 432

Query: 436 GLKIKAYISSSQNPTASISFKGTIIGDGSS--SPAMASFSSRGPSLSSPGILKPDITGPG 495
           GLKIK YI+SS NP ASISF+GT++G+ ++  SPAMASFSSRGP  +SPGILKPDITGPG
Sbjct: 433 GLKIKEYINSSHNPKASISFEGTLLGNRATTFSPAMASFSSRGPCQASPGILKPDITGPG 492

Query: 496 VNILAAWPFPLDN--NTDTRSTFNVISGTSMSCPHLSGIAALIKS-YP------------ 555
           VNILAAWPFPL+N  NT+T+STFNVISGTSMSCPHLSGIAALIKS +P            
Sbjct: 493 VNILAAWPFPLNNNTNTNTKSTFNVISGTSMSCPHLSGIAALIKSNHPNWSPAAIKSAIM 552

Query: 556 ------NPEGKPIVDQDLKPANLFAVGAGHVDPSRAADPGLVYDIRPDDYIPYLCGLYKD 615
                 NP+GKPIVDQDLKPAN FA+G+GHV+PS+AA+PGLVYDI+PDDY+PYLC LY D
Sbjct: 553 TSADVRNPQGKPIVDQDLKPANFFAMGSGHVNPSKAANPGLVYDIQPDDYVPYLCHLYTD 612

Query: 616 AEVSIIVQKPVICSLVPRIREGDLNYPSFSVALG-PLQTFKRTVTNVGDANSVYSAIIEA 618
           A+VSIIV++ V CS V RIREGDLNYPSF+V+LG   Q F RTVTNVGDANSVY AI++A
Sbjct: 613 AQVSIIVRRQVTCSTVSRIREGDLNYPSFAVSLGADSQAFNRTVTNVGDANSVYYAIVKA 672

BLAST of Sgr024350 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 456.1 bits (1172), Expect = 4.6e-128
Identity = 294/693 (42.42%), Postives = 387/693 (55.84%), Query Frame = 0

Query: 16  LQTYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLT 75
           L++YIVHV++     L    N   +W++S L  +  +S +   LLYSY   + GFSARL+
Sbjct: 30  LESYIVHVQRSHKPSLFSSHN---NWHVSLL-RSLPSSPQPATLLYSYSRAVHGFSARLS 89

Query: 76  EEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTGI 135
             Q  A+      IS  P+    +HTTHTPAFLG +Q  GLW +SN+GE VI+GVLDTGI
Sbjct: 90  PIQTAALRRHPSVISVIPDQAREIHTTHTPAFLGFSQNSGLWSNSNYGEDVIVGVLDTGI 149

Query: 136 TPTHPSFGDEGMSPPPAKWKGRCE----FNSSVCNNKLIGARTFNRA-----NGLWK--- 195
            P HPSF D G+ P P+ WKG CE    F +S CN KLIGAR F R      NG  K   
Sbjct: 150 WPEHPSFSDSGLGPIPSTWKGECEIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAA 209

Query: 196 --GESPADENGHGTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSS 255
               SP D  GHGTHTA TAAG+ V  A     A GTA GMA  A +A YK+C + GC  
Sbjct: 210 KESRSPRDTEGHGTHTASTAAGSVVANASLYQYARGTATGMASKARIAAYKICWTGGCYD 269

Query: 256 SDVLAGLDAAVDDGVDVLSLSLG-SRSAP-FFKDNVAIGTFAAIQ--------------- 315
           SD+LA +D AV DGV V+SLS+G S SAP +  D++AIG F A +               
Sbjct: 270 SDILAAMDQAVADGVHVISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPN 329

Query: 316 -------KEFLLVVRPEIQPSDFLS-----------------------TFLPLVYAGSNG 375
                    ++L V       +F +                       + L LVY+G  G
Sbjct: 330 PETATNIAPWILTVGASTVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSGDCG 389

Query: 376 NQTSAFCGQGSLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTF 435
              S  C  G L    V+GK+V+C  RGG  R+ KGS VK AGGA MIL N  E G    
Sbjct: 390 ---SRLCYPGKLNSSLVEGKIVLCD-RGGNARVEKGSAVKLAGGAGMILANTAESGEELT 449

Query: 436 SEAHVLPVSHVSYEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLS 495
           +++H++P + V  +AG +I+ YI +S +PTA ISF GT+IG    SP +A+FSSRGP+  
Sbjct: 450 ADSHLVPATMVGAKAGDQIRDYIKTSDSPTAKISFLGTLIGPSPPSPRVAAFSSRGPNHL 509

Query: 496 SPGILKPDITGPGVNILAAWP---FPLDNNTDTRST-FNVISGTSMSCPHLSGIAALI-K 555
           +P ILKPD+  PGVNILA W     P D + D R   FN+ISGTSMSCPH+SG+AAL+ K
Sbjct: 510 TPVILKPDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRK 569

Query: 556 SYPN------------------PEGKPIVDQDL-KPANLFAVGAGHVDPSRAADPGLVYD 615
           ++P+                    G+PI D    K +N F  GAGHVDP++A +PGLVYD
Sbjct: 570 AHPDWSPAAIKSALVTTAYDVENSGEPIEDLATGKSSNSFIHGAGHVDPNKALNPGLVYD 629

BLAST of Sgr024350 vs. TAIR 10
Match: AT1G04110.1 (Subtilase family protein )

HSP 1 Score: 446.4 bits (1147), Expect = 3.7e-125
Identity = 294/706 (41.64%), Postives = 392/706 (55.52%), Query Frame = 0

Query: 15  QLQTYIVHVKQPEIRLLGEETNDIESWYMSFLPET---TENSIEQP--RLLYSYRNVMSG 74
           Q QTYIV +  P         +  + W++SFL E     E   E+P  RLLYSY + + G
Sbjct: 24  QKQTYIVQL-HPNSETAKTFASKFD-WHLSFLQEAVLGVEEEEEEPSSRLLYSYGSAIEG 83

Query: 75  FSARLTEEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLN--QQFGLWKDSNFGEGVI 134
           F+A+LTE + E +      ++  P+ +L + TT++  FLGL+     G+W  S FG+G I
Sbjct: 84  FAAQLTESEAEILRYSPEVVAVRPDHVLQVQTTYSYKFLGLDGFGNSGVWSKSRFGQGTI 143

Query: 135 IGVLDTGITPTHPSFGDEGMSPPPAKWKGRCE----FNSSVCNNKLIGARTFNRANGLWK 194
           IGVLDTG+ P  PSF D GM   P KWKG C+    F+SS CN KLIGAR F R + +  
Sbjct: 144 IGVLDTGVWPESPSFDDTGMPSIPRKWKGICQEGESFSSSSCNRKLIGARFFIRGHRVAN 203

Query: 195 G--ESP---------ADENGHGTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYK 254
              ESP          D  GHGTHTA T  G+ V  A  LGN  G A GMAP AH+A+YK
Sbjct: 204 SPEESPNMPREYISARDSTGHGTHTASTVGGSSVSMANVLGNGAGVARGMAPGAHIAVYK 263

Query: 255 VCSSKGCSSSDVLAGLDAAVDDGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQ-------- 314
           VC   GC SSD+LA +D A+ D VDVLSLSLG    P + D +AIGTF A++        
Sbjct: 264 VCWFNGCYSSDILAAIDVAIQDKVDVLSLSLGGFPIPLYDDTIAIGTFRAMERGISVICA 323

Query: 315 ---------------------------KEFLLVVR---------PEIQPSDFLSTF---L 374
                                      + F  VVR           + P   +      +
Sbjct: 324 AGNNGPIESSVANTAPWVSTIGAGTLDRRFPAVVRLANGKLLYGESLYPGKGIKNAGREV 383

Query: 375 PLVYAGSNGNQTSAFCGQGSLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMIL-- 434
            ++Y  + G++ S FC +GSL   +++GK+V+C  RG  GR  KG  VK AGG AMIL  
Sbjct: 384 EVIYV-TGGDKGSEFCLRGSLPREEIRGKMVICD-RGVNGRSEKGEAVKEAGGVAMILAN 443

Query: 435 --INQQEDGFTTFSEAHVLPVSHVSYEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSP 494
             INQ+ED      + H+LP + + Y   + +KAY++++  P A I F GT+IG  S +P
Sbjct: 444 TEINQEEDSI----DVHLLPATLIGYTESVLLKAYVNATVKPKARIIFGGTVIG-RSRAP 503

Query: 495 AMASFSSRGPSLSSPGILKPDITGPGVNILAAWP-------FPLDNNTDTRSTFNVISGT 554
            +A FS+RGPSL++P ILKPD+  PGVNI+AAWP        P D+    R  F V+SGT
Sbjct: 504 EVAQFSARGPSLANPSILKPDMIAPGVNIIAAWPQNLGPTGLPYDSR---RVNFTVMSGT 563

Query: 555 SMSCPHLSGIAALIKS-YPN------------------PEGKPIVDQDLKPANLFAVGAG 614
           SMSCPH+SGI ALI+S YPN                   +GK I D + KPA +FA+GAG
Sbjct: 564 SMSCPHVSGITALIRSAYPNWSPAAIKSALMTTADLYDRQGKAIKDGN-KPAGVFAIGAG 623

Query: 615 HVDPSRAADPGLVYDIRPDDYIPYLCGL-YKDAEVSIIVQKPVICSLVPRIREG-DLNYP 617
           HV+P +A +PGLVY+I+P DYI YLC L +  +++  I  K V C+ + R   G  LNYP
Sbjct: 624 HVNPQKAINPGLVYNIQPVDYITYLCTLGFTRSDILAITHKNVSCNGILRKNPGFSLNYP 683

BLAST of Sgr024350 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 438.3 bits (1126), Expect = 1.0e-122
Identity = 281/685 (41.02%), Postives = 387/685 (56.50%), Query Frame = 0

Query: 18  TYIVHVKQPEIRLLGEETNDIES-WYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLTE 77
           TYIVH+ + ++      + D+ S WY S L   ++++     LLY+Y N + GFS RLT+
Sbjct: 31  TYIVHMAKSQM----PSSFDLHSNWYDSSLRSISDSA----ELLYTYENAIHGFSTRLTQ 90

Query: 78  EQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFG-LWKDSNFGEGVIIGVLDTGI 137
           E+ +++  + G IS  PE    LHTT TP FLGL++    L+ ++     V++GVLDTG+
Sbjct: 91  EEADSLMTQPGVISVLPEHRYELHTTRTPLFLGLDEHTADLFPEAGSYSDVVVGVLDTGV 150

Query: 138 TPTHPSFGDEGMSPPPAKWKGRCE----FNSSVCNNKLIGARTFNRANGLWKG------- 197
            P   S+ DEG  P P+ WKG CE    F +S+CN KLIGAR F R      G       
Sbjct: 151 WPESKSYSDEGFGPIPSSWKGGCEAGTNFTASLCNRKLIGARFFARGYESTMGPIDESKE 210

Query: 198 -ESPADENGHGTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSD 257
             SP D++GHGTHT+ TAAG+ V+GA  LG A GTA GMAP A +A+YKVC   GC SSD
Sbjct: 211 SRSPRDDDGHGTHTSSTAAGSVVEGASLLGYASGTARGMAPRARVAVYKVCWLGGCFSSD 270

Query: 258 VLAGLDAAVDDGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQKEFLLV------------- 317
           +LA +D A+ D V+VLS+SLG   + +++D VAIG FAA+++  L+              
Sbjct: 271 ILAAIDKAIADNVNVLSMSLGGGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSL 330

Query: 318 --VRPEIQ-------PSDF--------------LSTF---------LPLVYAGSNGNQTS 377
             V P I          DF              +S F         LP +YAG+  N T+
Sbjct: 331 SNVAPWITTVGAGTLDRDFPALAILGNGKNFTGVSLFKGEALPDKLLPFIYAGNASNATN 390

Query: 378 A-FCGQGSLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEA 437
              C  G+L    VKGK+V+C  RG   R+ KG VVK AGG  MIL N   +G    ++A
Sbjct: 391 GNLCMTGTLIPEKVKGKIVMCD-RGINARVQKGDVVKAAGGVGMILANTAANGEELVADA 450

Query: 438 HVLPVSHVSYEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSPG 497
           H+LP + V  +AG  I+ Y+++  NPTASIS  GT++G    SP +A+FSSRGP+  +P 
Sbjct: 451 HLLPATTVGEKAGDIIRHYVTTDPNPTASISILGTVVG-VKPSPVVAAFSSRGPNSITPN 510

Query: 498 ILKPDITGPGVNILAAW---PFPLDNNTDTRST-FNVISGTSMSCPHLSGIAALIKS-YP 557
           ILKPD+  PGVNILAAW     P    +D+R   FN+ISGTSMSCPH+SG+AAL+KS +P
Sbjct: 511 ILKPDLIAPGVNILAAWTGAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHP 570

Query: 558 N------------------PEGKPIVD-QDLKPANLFAVGAGHVDPSRAADPGLVYDIRP 616
                               +GKP++D    KP+  F  GAGHV P+ A +PGL+YD+  
Sbjct: 571 EWSPAAIRSALMTTAYKTYKDGKPLLDIATGKPSTPFDHGAGHVSPTTATNPGLIYDLTT 630

BLAST of Sgr024350 vs. TAIR 10
Match: AT1G01900.1 (subtilase family protein )

HSP 1 Score: 426.8 bits (1096), Expect = 3.0e-119
Identity = 275/691 (39.80%), Postives = 374/691 (54.12%), Query Frame = 0

Query: 17  QTYIVHVKQPEIRLLGEETNDIESWYMSFLPET-TENSIEQPRLLYSYRNVMSGFSARLT 76
           QTY++H      + +      + S + S   E   ++    P + Y Y N MSGFSA LT
Sbjct: 42  QTYVIHTVTTSTKHI------VTSLFNSLQTENINDDDFSLPEIHYIYENAMSGFSATLT 101

Query: 77  EEQVEAMEGKDGFISATPETILSLHTTHTPAFLGLNQQFGLWKDSNFGEGVIIGVLDTGI 136
           ++Q++ ++   GFISA P+ +LSLHTT++  FLGL    GLW +++    VIIG++DTGI
Sbjct: 102 DDQLDTVKNTKGFISAYPDELLSLHTTYSHEFLGLEFGIGLWNETSLSSDVIIGLVDTGI 161

Query: 137 TPTHPSFGDEGMSPPPAKWKGRCE----FNSSVCNNKLIGARTF--------NRANGLWK 196
           +P H SF D  M+P P++W+G C+    F+SS CN K+IGA  F         + N    
Sbjct: 162 SPEHVSFRDTHMTPVPSRWRGSCDEGTNFSSSECNKKIIGASAFYKGYESIVGKINETTD 221

Query: 197 GESPADENGHGTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSD 256
             S  D  GHGTHTA TAAG  V  A   G A+G A GM   + +A YK C + GC+S+D
Sbjct: 222 FRSTRDAQGHGTHTASTAAGDIVPKANYFGQAKGLASGMRFTSRIAAYKACWALGCASTD 281

Query: 257 VLAGLDAAVDDGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQKE----------------- 316
           V+A +D A+ DGVDV+SLSLG  S PF+ D +AI  F A+QK                  
Sbjct: 282 VIAAIDRAILDGVDVISLSLGGSSRPFYVDPIAIAGFGAMQKNIFVSCSAGNSGPTASTV 341

Query: 317 ------------------FLLVVRPEIQPSDFLSTF--------LPLVYAGSNGNQTSA- 376
                             F  +VR   + S   S+         LPL +  + G ++ A 
Sbjct: 342 SNGAPWLMTVAASYTDRTFPAIVRIGNRKSLVGSSLYKGKSLKNLPLAFNRTAGEESGAV 401

Query: 377 FCGQGSLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTFSEAHV 436
           FC + SL    V+GK+V+C  RG  GR AKG  VK +GGAAM+L++ + +G    ++ HV
Sbjct: 402 FCIRDSLKRELVEGKIVICL-RGASGRTAKGEEVKRSGGAAMLLVSTEAEGEELLADPHV 461

Query: 437 LPVSHVSYEAGLKIKAYISSSQNPTASISFKGTIIGDGSSSPAMASFSSRGPSLSSPGIL 496
           LP   + +  G  +  Y++ + N TAS+ F+GT    G+++P +A+FSSRGPS++ P I 
Sbjct: 462 LPAVSLGFSDGKTLLNYLAGAANATASVRFRGTAY--GATAPMVAAFSSRGPSVAGPEIA 521

Query: 497 KPDITGPGVNILAAW-PF---PLDNNTDTRSTFNVISGTSMSCPHLSGIAALIKS----- 556
           KPDI  PG+NILA W PF    L  +   R  FN+ISGTSM+CPH+SGIAALIKS     
Sbjct: 522 KPDIAAPGLNILAGWSPFSSPSLLRSDPRRVQFNIISGTSMACPHISGIAALIKSVHGDW 581

Query: 557 --------------YPNPEGKPIVDQDL----KPANLFAVGAGHVDPSRAADPGLVYDIR 616
                           +   +PI D+        A  FA GAG+VDP+RA DPGLVYD  
Sbjct: 582 SPAMIKSAIMTTARITDNRNRPIGDRGAAGAESAATAFAFGAGNVDPTRAVDPGLVYDTS 641

BLAST of Sgr024350 vs. TAIR 10
Match: AT3G14240.1 (Subtilase family protein )

HSP 1 Score: 419.1 bits (1076), Expect = 6.3e-117
Identity = 280/703 (39.83%), Postives = 373/703 (53.06%), Query Frame = 0

Query: 18  TYIVHVKQPEIRLLGEETNDIESWYMSFLPETTENSIEQPRLLYSYRNVMSGFSARLTEE 77
           TYIVHV   E +     T+    WY S L   T +    P ++++Y  V  GFSARLT +
Sbjct: 27  TYIVHVDH-EAKPSIFPTH--FHWYTSSLASLTSS---PPSIIHTYDTVFHGFSARLTSQ 86

Query: 78  QVEAMEGKDGFISATPETILSLHTTHTPAFLGL--NQQFGLWKDSNFGEGVIIGVLDTGI 137
               +      IS  PE +  LHTT +P FLGL    + GL ++S+FG  ++IGV+DTG+
Sbjct: 87  DASQLLDHPHVISVIPEQVRHLHTTRSPEFLGLRSTDKAGLLEESDFGSDLVIGVIDTGV 146

Query: 138 TPTHPSFGDEGMSPPPAKWKGRC----EFNSSVCNNKLIGARTF--------NRANGLWK 197
            P  PSF D G+ P P KWKG+C    +F  S CN KL+GAR F         + N   +
Sbjct: 147 WPERPSFDDRGLGPVPIKWKGQCIASQDFPESACNRKLVGARFFCGGYEATNGKMNETTE 206

Query: 198 GESPADENGHGTHTAGTAAGAFVKGAEALGNAEGTAVGMAPLAHLAMYKVCSSKGCSSSD 257
             SP D +GHGTHTA  +AG +V  A  LG A G A GMAP A LA YKVC + GC  SD
Sbjct: 207 FRSPRDSDGHGTHTASISAGRYVFPASTLGYAHGVAAGMAPKARLAAYKVCWNSGCYDSD 266

Query: 258 VLAGLDAAVDDGVDVLSLSLGSRSAPFFKDNVAIGTFAAIQK------------------ 317
           +LA  D AV DGVDV+SLS+G    P++ D +AIG F AI +                  
Sbjct: 267 ILAAFDTAVADGVDVISLSVGGVVVPYYLDAIAIGAFGAIDRGIFVSASAGNGGPGALTV 326

Query: 318 ----EFLLVVRPEIQPSDFLST------------------------FLPLVYAGS---NG 377
                ++  V       DF +                           PLVY GS     
Sbjct: 327 TNVAPWMTTVGAGTIDRDFPANVKLGNGKMISGVSVYGGPGLDPGRMYPLVYGGSLLGGD 386

Query: 378 NQTSAFCGQGSLTDIDVKGKVVVCQGRGGVGRIAKGSVVKNAGGAAMILINQQEDGFTTF 437
             +S+ C +GSL    VKGK+V+C  RG   R  KG +V+  GG  MI+ N   DG    
Sbjct: 387 GYSSSLCLEGSLDPNLVKGKIVLCD-RGINSRATKGEIVRKNGGLGMIIANGVFDGEGLV 446

Query: 438 SEAHVLPVSHVSYEAGLKIKAYIS------SSQNPTASISFKGTIIGDGSSSPAMASFSS 497
           ++ HVLP + V    G +I+ YIS      SS++PTA+I FKGT +G    +P +ASFS+
Sbjct: 447 ADCHVLPATSVGASGGDEIRRYISESSKSRSSKHPTATIVFKGTRLGI-RPAPVVASFSA 506

Query: 498 RGPSLSSPGILKPDITGPGVNILAAWP---FPLDNNTDTRST-FNVISGTSMSCPHLSGI 557
           RGP+  +P ILKPD+  PG+NILAAWP    P    +D R T FN++SGTSM+CPH+SG+
Sbjct: 507 RGPNPETPEILKPDVIAPGLNILAAWPDRIGPSGVTSDNRRTEFNILSGTSMACPHVSGL 566

Query: 558 AALIK-SYP------------------NPEGKPIVDQDL-KPANLFAVGAGHVDPSRAAD 617
           AAL+K ++P                  +  G+P++D+     +++   G+GHV P++A D
Sbjct: 567 AALLKAAHPDWSPAAIRSALITTAYTVDNSGEPMMDESTGNTSSVMDYGSGHVHPTKAMD 626

Query: 618 PGLVYDIRPDDYIPYLCGL-YKDAEVSIIVQKPVICSLVPRI-REGDLNYPSFSVAL--- 620
           PGLVYDI   DYI +LC   Y    +  I ++   C    R    G+LNYPSFSV     
Sbjct: 627 PGLVYDITSYDYINFLCNSNYTRTNIVTITRRQADCDGARRAGHVGNLNYPSFSVVFQQY 686