Sgr024309 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr024309
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	tig00001291: 1641746 .. 1642372 (-)
RNA-Seq Expression	Sgr024309
Synteny	Sgr024309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAAAGCTCAAGATCTCATCAGTACTTCTCTTCCTTTTGGTCCTCCACAATGCCGCCGCCGCAGCAGACTCAACCGCAACCGCAACTTCAACCCCCTCCCCCACGAGCTTCATCGAGTCATCCTGCAGAGTCACTCGCTACCCAGCTCTGTGCGTTCAATCCCTCTCCGGCTACGCCGGAGCCATCCGCCAAAGCGGGCGGCAGCTGGCCCAAACGGCCCTGTCCGTGAGCTTGAGCAAGGCCCGTCAAGCCGCAGCGTTCGTCGCCAAGCTCGGGAGAGGCGGCGGAATGAGAGGCCTGGAGTACCAAGCGGTGAAAGACTGCATAGAAAACATGGGCGACAGCGTGGACCGGCTCAGCCAATCGGTGAAGGAGCTTGGGAACTTACGTCGAACAGCTGGTGGGGATTTCTTGTGGCACATGAGTAACGTCCAGACATGGGTCAGCGCCGCCCTGACTGATGAAAGCACCTGCCTTGATGGGTTCGCCGGCCGTCGCATGGACGGCCAAGTGAAGGCGACAATTAGGCGCAGAATCACCGTTGTTGCTCAAGTCACTAGCAATGCCCTCGCTCTGGTTAATCGCTTTGCAGACAAAAACCACGTCGCCATGGCCATGCCTTAG

mRNA sequence

ATGGCAAAGCTCAAGATCTCATCAGTACTTCTCTTCCTTTTGGTCCTCCACAATGCCGCCGCCGCAGCAGACTCAACCGCAACCGCAACTTCAACCCCCTCCCCCACGAGCTTCATCGAGTCATCCTGCAGAGTCACTCGCTACCCAGCTCTGTGCGTTCAATCCCTCTCCGGCTACGCCGGAGCCATCCGCCAAAGCGGGCGGCAGCTGGCCCAAACGGCCCTGTCCGTGAGCTTGAGCAAGGCCCGTCAAGCCGCAGCGTTCGTCGCCAAGCTCGGGAGAGGCGGCGGAATGAGAGGCCTGGAGTACCAAGCGGTGAAAGACTGCATAGAAAACATGGGCGACAGCGTGGACCGGCTCAGCCAATCGGTGAAGGAGCTTGGGAACTTACGTCGAACAGCTGGTGGGGATTTCTTGTGGCACATGAGTAACGTCCAGACATGGGTCAGCGCCGCCCTGACTGATGAAAGCACCTGCCTTGATGGGTTCGCCGGCCGTCGCATGGACGGCCAAGTGAAGGCGACAATTAGGCGCAGAATCACCGTTGTTGCTCAAGTCACTAGCAATGCCCTCGCTCTGGTTAATCGCTTTGCAGACAAAAACCACGTCGCCATGGCCATGCCTTAG

Coding sequence (CDS)

ATGGCAAAGCTCAAGATCTCATCAGTACTTCTCTTCCTTTTGGTCCTCCACAATGCCGCCGCCGCAGCAGACTCAACCGCAACCGCAACTTCAACCCCCTCCCCCACGAGCTTCATCGAGTCATCCTGCAGAGTCACTCGCTACCCAGCTCTGTGCGTTCAATCCCTCTCCGGCTACGCCGGAGCCATCCGCCAAAGCGGGCGGCAGCTGGCCCAAACGGCCCTGTCCGTGAGCTTGAGCAAGGCCCGTCAAGCCGCAGCGTTCGTCGCCAAGCTCGGGAGAGGCGGCGGAATGAGAGGCCTGGAGTACCAAGCGGTGAAAGACTGCATAGAAAACATGGGCGACAGCGTGGACCGGCTCAGCCAATCGGTGAAGGAGCTTGGGAACTTACGTCGAACAGCTGGTGGGGATTTCTTGTGGCACATGAGTAACGTCCAGACATGGGTCAGCGCCGCCCTGACTGATGAAAGCACCTGCCTTGATGGGTTCGCCGGCCGTCGCATGGACGGCCAAGTGAAGGCGACAATTAGGCGCAGAATCACCGTTGTTGCTCAAGTCACTAGCAATGCCCTCGCTCTGGTTAATCGCTTTGCAGACAAAAACCACGTCGCCATGGCCATGCCTTAG

Protein sequence

MAKLKISSVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYAGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRLSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVAQVTSNALALVNRFADKNHVAMAMP

Homology

[Close]

BLAST of Sgr024309 vs. NCBI nr
Match: XP_038875547.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 308.1 bits (788), Expect = 5.6e-80
Identity = 164/203 (80.79%), Postives = 179/203 (88.18%), Query Frame = 0


Query: 1   MAKLKIS-SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  +L+ L+ LHNAA A  S A        TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 13  MANLKISLPILIILVALHNAATAESSAA--------TSFIESSCKVTRYPALCVQSLSSY 72

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+ ALSVSLSKAR AAAFVAKLG+GGGM+GLEYQAVKDCIENMGDSVDR
Sbjct: 73  ATTIRQSGRQLARMALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDR 132

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           L QSVKELG+LRRTAGGDFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ+KA IR+R
Sbjct: 133 LGQSVKELGDLRRTAGGDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAAIRQR 192

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFADKNH
Sbjct: 193 ITLVAQITSNALALVNRFADKNH 207

[Close]

BLAST of Sgr024309 vs. NCBI nr
Match: XP_022156444.1 (21 kDa protein-like [Momordica charantia])

HSP 1 Score: 305.1 bits (780), Expect = 4.7e-79
Identity = 169/209 (80.86%), Postives = 181/209 (86.60%), Query Frame = 0


Query: 1   MAKLKISSVLLFLL--VLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSG 60
           MA+LKIS    FL+  VLHNAAA       A S  SPTSFIESSCRVTRYP LCV+SLSG
Sbjct: 1   MARLKISLPFAFLILAVLHNAAA-------ADSASSPTSFIESSCRVTRYPTLCVESLSG 60

Query: 61  YAGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLG-RGGGMRGLEYQAVKDCIENMGDSV 120
           YA AIRQSGRQLAQTALSVSLS+ARQAA FVA LG R G MRGLEYQAVKDCIENMGDSV
Sbjct: 61  YASAIRQSGRQLAQTALSVSLSRARQAATFVAGLGKRRGPMRGLEYQAVKDCIENMGDSV 120

Query: 121 DRLSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIR 180
           D L +SVKELG+LRRTAGGDFLWHMSNVQTWVSAALTDE+TCLDGFAGRR DGQVKA+IR
Sbjct: 121 DSLRRSVKELGDLRRTAGGDFLWHMSNVQTWVSAALTDETTCLDGFAGRRTDGQVKASIR 180

Query: 181 RRITVVAQVTSNALALVNRFADKNHVAMA 207
           +++T+VAQVTSNALALVN FADKNHVA A
Sbjct: 181 KKVTLVAQVTSNALALVNHFADKNHVARA 202

[Close]

BLAST of Sgr024309 vs. NCBI nr
Match: KAA0039678.1 (21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 303.5 bits (776), Expect = 1.4e-78
Identity = 165/203 (81.28%), Postives = 180/203 (88.67%), Query Frame = 0


Query: 1   MAKLKIS-SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  +L+ L+ LHNAA         T +PS TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 1   MANLKISLPLLITLIALHNAA--------TTGSPS-TSFIESSCKVTRYPALCVQSLSTY 60

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+TALSVSLSKAR AAAFVAKLG+GGGM+GLEYQAVKDCIENMGDSVDR
Sbjct: 61  ANTIRQSGRQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDR 120

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           LSQSVKELG+LRRTAG DFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ KA IRRR
Sbjct: 121 LSQSVKELGDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRR 180

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFAD+NH
Sbjct: 181 ITLVAQITSNALALVNRFADENH 194

[Close]

BLAST of Sgr024309 vs. NCBI nr
Match: XP_008437134.1 (PREDICTED: 21 kDa protein-like [Cucumis melo])

HSP 1 Score: 300.8 bits (769), Expect = 9.0e-78
Identity = 164/203 (80.79%), Postives = 179/203 (88.18%), Query Frame = 0


Query: 1   MAKLKIS-SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  +L+ L+ LHNAA         T +PS TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 1   MANLKISLPLLITLIALHNAA--------TTGSPS-TSFIESSCKVTRYPALCVQSLSTY 60

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+TALSVSLSKA  AAAFVAKLG+GGGM+GLEYQAVKDCIENMGDSVDR
Sbjct: 61  ANTIRQSGRQLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDR 120

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           LSQSVKELG+LRRTAG DFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ KA IRRR
Sbjct: 121 LSQSVKELGDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRR 180

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFAD+NH
Sbjct: 181 ITLVAQITSNALALVNRFADENH 194

[Close]

BLAST of Sgr024309 vs. NCBI nr
Match: XP_004147584.1 (21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cucumis sativus])

HSP 1 Score: 300.8 bits (769), Expect = 9.0e-78
Identity = 162/203 (79.80%), Postives = 179/203 (88.18%), Query Frame = 0


Query: 1   MAKLKISSVLLFLLV-LHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  LL  LV LHNA         AT+  + TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 1   MANLKISLPLLITLVALHNA---------ATTGSAATSFIESSCKVTRYPALCVQSLSTY 60

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+TALSVSLSKAR A+AFVAKLG+GGGM+GLEYQAVKDCIENMGD+VDR
Sbjct: 61  ANVIRQSGRQLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDR 120

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           LSQSVKELG+LR+TAG DFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ+KA IRRR
Sbjct: 121 LSQSVKELGDLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRR 180

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFAD+NH
Sbjct: 181 ITLVAQITSNALALVNRFADENH 194

[Close]

BLAST of Sgr024309 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 7.9e-45
Identity = 108/196 (55.10%), Postives = 137/196 (69.90%), Query Frame = 0


Query: 11  LFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYAGAIRQSGRQ- 70
           +FL++L +      S+AT   + S   FI SSC+ T+YP+LCV +LS YA  IR +  Q 
Sbjct: 7   IFLVILLSITILQSSSATPNRSES-DQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQD 66

Query: 71  LAQTALSVSLSKARQAAAFVAKLGR-GGGMRGLEYQAVKDCIENMGDSVDRLSQSVKELG 130
           LAQTAL +SL++A+    FVAKL +     +  EY A+KDCIE +G+SVDRL+QSVKEL 
Sbjct: 67  LAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA 126

Query: 131 NL-RRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVAQVT 190
                 A  DF+W MSNVQTWVSAALTDE+TCLDGF+ R M G+VK  IR ++  VAQVT
Sbjct: 127 RAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 186

Query: 191 SNALALVNRFADKNHV 204
           SNALALVN+FA+K  V
Sbjct: 187 SNALALVNQFAEKRSV 201

[Close]

BLAST of Sgr024309 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 1.8e-44
Identity = 99/201 (49.25%), Postives = 142/201 (70.65%), Query Frame = 0


Query: 4   LKIS-SVLLF--LLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYA 63
           +K+S S L+F  LLV+  AA+AA +            FI++SC +T YPA+C QSLS YA
Sbjct: 1   MKLSKSTLVFSALLVILAAASAAPA----------NQFIKTSCTLTTYPAVCEQSLSAYA 60

Query: 64  GAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRL 123
             I+ + ++LA TAL VSL++ +QA  F+ +L +  G++  +Y A+ DC+E + DS+DR+
Sbjct: 61  KTIQNNPQELASTALQVSLTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRV 120

Query: 124 SQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRI 183
           S+S  E+ NL    G DF + MSNV+TWVSAALTDE+TC+DGFAG+ MDG++K ++R ++
Sbjct: 121 SRSCDEMKNLSHAKGNDFTFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQV 180

Query: 184 TVVAQVTSNALALVNRFADKN 202
             VA+VTSNALALVN FA K+
Sbjct: 181 VAVARVTSNALALVNNFAAKH 191

[Close]

BLAST of Sgr024309 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 1.8e-41
Identity = 96/201 (47.76%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MAKLKISSVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYA 60
           MAK    ++ LFLL      A++ +   A ++    +FI++SC+ T YP +CV SL+GYA
Sbjct: 1   MAKQIFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYA 60

Query: 61  GAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRL 120
            +I+ S R+LA+TAL+V++++A+    FV +LGR   ++  E QAVKDCIE + D+VDRL
Sbjct: 61  NSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL 120

Query: 121 SQSVKELGNLRRTAGGDFLW-HMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           + S+ E+       G D  W HMSN QTW SAALT+ +TC DGFAGR MDG+VK ++R R
Sbjct: 121 TMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRAR 180

Query: 181 ITVVAQVTSNALALVNRFADK 201
           I  + + TSNALAL+N FA K
Sbjct: 181 ILNLGRGTSNALALINAFAKK 201

[Close]

BLAST of Sgr024309 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.5e-40
Identity = 93/195 (47.69%), Postives = 133/195 (68.21%), Query Frame = 0


Query: 8   SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYAGAIRQSG 67
           S++LF+L L  AA          S+   T FI++SC  T YP  C QSLS YA  I++  
Sbjct: 8   SLILFVLYLSTAAIVMARNLEEESS-GDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 67

Query: 68  RQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRLSQSVKEL 127
           R+LA+TAL+VS+++A+ A  +V+++    G+   +++AV DC+E MGD+VDRLS S+KEL
Sbjct: 68  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 127

Query: 128 GNLRR-TAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVAQV 187
            +L    +G DF + +SNV+TW SAALTDE+ C+DGF G+ M G++K+ IR  I  VA+ 
Sbjct: 128 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 187

Query: 188 TSNALALVNRFADKN 202
           TSNALAL+N FA K+
Sbjct: 188 TSNALALINDFASKH 201

[Close]

BLAST of Sgr024309 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 7.7e-32
Identity = 75/180 (41.67%), Postives = 116/180 (64.44%), Query Frame = 0


Query: 19  AAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYAGAIRQSGRQLAQTALSVS 78
           ++ +  S+++  S  +   +I++SC +T Y  +C  SLS YA  IR + ++LA  AL+++
Sbjct: 128 SSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNLT 187

Query: 79  LSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRLSQSVKELGNLRRTAGGDF 138
           LS A+ A+ FV  +  GGG+  LE  AV DC+E +GDSV  L  S++EL ++       F
Sbjct: 188 LSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAKF 247

Query: 139 LWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVAQVTSNALALVNRFA 198
              MS+V+TWVSAALT++ TC+DGF+   +   VK  +RR +  VA++TSNALAL+N +A
Sbjct: 248 EMVMSDVETWVSAALTNDDTCMDGFS--LVKTAVKDLVRRHVVEVARLTSNALALINMYA 305

[Close]

BLAST of Sgr024309 vs. ExPASy TrEMBL
Match: A0A6J1DTH0 (21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111023336 PE=4 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 2.3e-79
Identity = 169/209 (80.86%), Postives = 181/209 (86.60%), Query Frame = 0


Query: 1   MAKLKISSVLLFLL--VLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSG 60
           MA+LKIS    FL+  VLHNAAA       A S  SPTSFIESSCRVTRYP LCV+SLSG
Sbjct: 1   MARLKISLPFAFLILAVLHNAAA-------ADSASSPTSFIESSCRVTRYPTLCVESLSG 60

Query: 61  YAGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLG-RGGGMRGLEYQAVKDCIENMGDSV 120
           YA AIRQSGRQLAQTALSVSLS+ARQAA FVA LG R G MRGLEYQAVKDCIENMGDSV
Sbjct: 61  YASAIRQSGRQLAQTALSVSLSRARQAATFVAGLGKRRGPMRGLEYQAVKDCIENMGDSV 120

Query: 121 DRLSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIR 180
           D L +SVKELG+LRRTAGGDFLWHMSNVQTWVSAALTDE+TCLDGFAGRR DGQVKA+IR
Sbjct: 121 DSLRRSVKELGDLRRTAGGDFLWHMSNVQTWVSAALTDETTCLDGFAGRRTDGQVKASIR 180

Query: 181 RRITVVAQVTSNALALVNRFADKNHVAMA 207
           +++T+VAQVTSNALALVN FADKNHVA A
Sbjct: 181 KKVTLVAQVTSNALALVNHFADKNHVARA 202

[Close]

BLAST of Sgr024309 vs. ExPASy TrEMBL
Match: A0A5A7T9V3 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G00090 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 6.7e-79
Identity = 165/203 (81.28%), Postives = 180/203 (88.67%), Query Frame = 0


Query: 1   MAKLKIS-SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  +L+ L+ LHNAA         T +PS TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 1   MANLKISLPLLITLIALHNAA--------TTGSPS-TSFIESSCKVTRYPALCVQSLSTY 60

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+TALSVSLSKAR AAAFVAKLG+GGGM+GLEYQAVKDCIENMGDSVDR
Sbjct: 61  ANTIRQSGRQLARTALSVSLSKARLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDR 120

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           LSQSVKELG+LRRTAG DFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ KA IRRR
Sbjct: 121 LSQSVKELGDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRR 180

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFAD+NH
Sbjct: 181 ITLVAQITSNALALVNRFADENH 194

[Close]

BLAST of Sgr024309 vs. ExPASy TrEMBL
Match: A0A0A0KR60 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.3e-78
Identity = 162/203 (79.80%), Postives = 179/203 (88.18%), Query Frame = 0


Query: 1   MAKLKISSVLLFLLV-LHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  LL  LV LHNA         AT+  + TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 1   MANLKISLPLLITLVALHNA---------ATTGSAATSFIESSCKVTRYPALCVQSLSTY 60

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+TALSVSLSKAR A+AFVAKLG+GGGM+GLEYQAVKDCIENMGD+VDR
Sbjct: 61  ANVIRQSGRQLARTALSVSLSKARLASAFVAKLGKGGGMKGLEYQAVKDCIENMGDTVDR 120

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           LSQSVKELG+LR+TAG DFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ+KA IRRR
Sbjct: 121 LSQSVKELGDLRQTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQIKAEIRRR 180

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFAD+NH
Sbjct: 181 ITLVAQITSNALALVNRFADENH 194

[Close]

BLAST of Sgr024309 vs. ExPASy TrEMBL
Match: A0A1S3ATE5 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 4.3e-78
Identity = 164/203 (80.79%), Postives = 179/203 (88.18%), Query Frame = 0


Query: 1   MAKLKIS-SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGY 60
           MA LKIS  +L+ L+ LHNAA         T +PS TSFIESSC+VTRYPALCVQSLS Y
Sbjct: 1   MANLKISLPLLITLIALHNAA--------TTGSPS-TSFIESSCKVTRYPALCVQSLSTY 60

Query: 61  AGAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDR 120
           A  IRQSGRQLA+TALSVSLSKA  AAAFVAKLG+GGGM+GLEYQAVKDCIENMGDSVDR
Sbjct: 61  ANTIRQSGRQLARTALSVSLSKALLAAAFVAKLGKGGGMKGLEYQAVKDCIENMGDSVDR 120

Query: 121 LSQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           LSQSVKELG+LRRTAG DFLWHM+NVQTWVSAALTDE+TCLDGFAGRR+DGQ KA IRRR
Sbjct: 121 LSQSVKELGDLRRTAGRDFLWHMNNVQTWVSAALTDETTCLDGFAGRRLDGQTKAGIRRR 180

Query: 181 ITVVAQVTSNALALVNRFADKNH 203
           IT+VAQ+TSNALALVNRFAD+NH
Sbjct: 181 ITLVAQITSNALALVNRFADENH 194

[Close]

BLAST of Sgr024309 vs. ExPASy TrEMBL
Match: A0A6J1H2L8 (pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 3.2e-73
Identity = 153/202 (75.74%), Postives = 169/202 (83.66%), Query Frame = 0


Query: 1   MAKLKISSVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYA 60
           M  LKIS  LL L+VLHNAA    +TA        TSFIESSCRVTRYPALCV SLS YA
Sbjct: 1   MPNLKISLPLLVLVVLHNAAPTHSATA--------TSFIESSCRVTRYPALCVHSLSAYA 60

Query: 61  GAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRL 120
            +IRQS RQL QTALSVSL+K+R AAAFV+ L +GG +RGLEYQA+KDCIENMGD+VDRL
Sbjct: 61  TSIRQSSRQLTQTALSVSLNKSRLAAAFVSNLAKGGAVRGLEYQALKDCIENMGDTVDRL 120

Query: 121 SQSVKELGNLRRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRI 180
            QSVKEL +LRRTAGGD LWH+SNVQTWVSAALTDE+TCLDGFA RR+DGQVKA IRR+I
Sbjct: 121 RQSVKELRDLRRTAGGDLLWHLSNVQTWVSAALTDETTCLDGFASRRLDGQVKAVIRRKI 180

Query: 181 TVVAQVTSNALALVNRFADKNH 203
           T+VAQVTSNALALVN FADK H
Sbjct: 181 TLVAQVTSNALALVNHFADKKH 194

[Close]

BLAST of Sgr024309 vs. TAIR 10
Match: AT4G12390.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 183.0 bits (463), Expect = 2.5e-46
Identity = 101/197 (51.27%), Postives = 140/197 (71.07%), Query Frame = 0


Query: 10  LLFLLVLHNAAAAADSTATATSTPSPTS---FIESSCRVTRYPALCVQSLSGYAGAIRQS 69
           L + L+L        + A  +S+P+P+S   FI SSCRVTRY  LCV+ L+ +A  IR++
Sbjct: 8   LCYCLLLFLPLLCQSTIAKPSSSPNPSSSINFIVSSCRVTRYQTLCVKCLAAFADKIRRN 67

Query: 70  GRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRLSQSVKE 129
             QLAQTAL+V+L + +    +V KL +   ++  EY AVKDC+EN+GD ++ L+QS++E
Sbjct: 68  ENQLAQTALAVTLVRVQSTTIYVGKLTKARRIKRREYLAVKDCVENLGDGLEMLAQSMRE 127

Query: 130 LGNLRRTA--GGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVA 189
           L  + R+     +FLW +SNV+TWVSAALTDE+TCLDGF G+ MDG VK+ IRRR+  VA
Sbjct: 128 LKQVGRSGRDRDEFLWRLSNVETWVSAALTDETTCLDGFDGKVMDGVVKSAIRRRVVHVA 187

Query: 190 QVTSNALALVNRFADKN 202
           +VTSNALALVNRFA ++
Sbjct: 188 RVTSNALALVNRFAARH 204

[Close]

BLAST of Sgr024309 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 181.8 bits (460), Expect = 5.6e-46
Identity = 108/196 (55.10%), Postives = 137/196 (69.90%), Query Frame = 0


Query: 11  LFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYAGAIRQSGRQ- 70
           +FL++L +      S+AT   + S   FI SSC+ T+YP+LCV +LS YA  IR +  Q 
Sbjct: 7   IFLVILLSITILQSSSATPNRSES-DQFIVSSCQTTQYPSLCVHTLSAYATKIRHNNDQD 66

Query: 71  LAQTALSVSLSKARQAAAFVAKLGR-GGGMRGLEYQAVKDCIENMGDSVDRLSQSVKELG 130
           LAQTAL +SL++A+    FVAKL +     +  EY A+KDCIE +G+SVDRL+QSVKEL 
Sbjct: 67  LAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSVKELA 126

Query: 131 NL-RRTAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVAQVT 190
                 A  DF+W MSNVQTWVSAALTDE+TCLDGF+ R M G+VK  IR ++  VAQVT
Sbjct: 127 RAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHVAQVT 186

Query: 191 SNALALVNRFADKNHV 204
           SNALALVN+FA+K  V
Sbjct: 187 SNALALVNQFAEKRSV 201

[Close]

BLAST of Sgr024309 vs. TAIR 10
Match: AT3G47380.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 170.6 bits (431), Expect = 1.3e-42
Identity = 96/201 (47.76%), Postives = 135/201 (67.16%), Query Frame = 0


Query: 1   MAKLKISSVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYA 60
           MAK    ++ LFLL      A++ +   A ++    +FI++SC+ T YP +CV SL+GYA
Sbjct: 1   MAKQIFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYA 60

Query: 61  GAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRL 120
            +I+ S R+LA+TAL+V++++A+    FV +LGR   ++  E QAVKDCIE + D+VDRL
Sbjct: 61  NSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL 120

Query: 121 SQSVKELGNLRRTAGGDFLW-HMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           + S+ E+       G D  W HMSN QTW SAALT+ +TC DGFAGR MDG+VK ++R R
Sbjct: 121 TMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRAR 180

Query: 181 ITVVAQVTSNALALVNRFADK 201
           I  + + TSNALAL+N FA K
Sbjct: 181 ILNLGRGTSNALALINAFAKK 201

[Close]

BLAST of Sgr024309 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 167.9 bits (424), Expect = 8.4e-42
Identity = 95/199 (47.74%), Postives = 134/199 (67.34%), Query Frame = 0


Query: 1   MAKLKISSVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYA 60
           MAK  +  +L    +L     AA + +   ++    +FI+SSC+ T YPALCV SLS YA
Sbjct: 1   MAKQYLFVLLSISYLLSLELTAATAASQTGASKKAINFIQSSCKTTTYPALCVHSLSVYA 60

Query: 61  GAIRQSGRQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRL 120
             I+ S ++LA+TA++V+LS+A+    FV++L R  G++  E +A+KDC+E M D+VDRL
Sbjct: 61  NDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKGLKKREVEAIKDCVEEMNDTVDRL 120

Query: 121 SQSVKELGNLRRTAGGD-FLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRR 180
           ++SV+EL         D F +HMSN QTW SAALTDE+TC DGF+GR MDG++K ++R R
Sbjct: 121 TKSVQELKLCGSAKDQDQFAYHMSNAQTWTSAALTDENTCSDGFSGRVMDGRIKNSVRAR 180

Query: 181 ITVVAQVTSNALALVNRFA 199
           I  V   TSNAL+L+N FA
Sbjct: 181 IMNVGHETSNALSLINAFA 199

[Close]

BLAST of Sgr024309 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.5e-41
Identity = 93/195 (47.69%), Postives = 133/195 (68.21%), Query Frame = 0


Query: 8   SVLLFLLVLHNAAAAADSTATATSTPSPTSFIESSCRVTRYPALCVQSLSGYAGAIRQSG 67
           S++LF+L L  AA          S+   T FI++SC  T YP  C QSLS YA  I++  
Sbjct: 8   SLILFVLYLSTAAIVMARNLEEESS-GDTEFIKASCETTSYPDRCFQSLSSYASEIKKQP 67

Query: 68  RQLAQTALSVSLSKARQAAAFVAKLGRGGGMRGLEYQAVKDCIENMGDSVDRLSQSVKEL 127
           R+LA+TAL+VS+++A+ A  +V+++    G+   +++AV DC+E MGD+VDRLS S+KEL
Sbjct: 68  RKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSLKEL 127

Query: 128 GNLRR-TAGGDFLWHMSNVQTWVSAALTDESTCLDGFAGRRMDGQVKATIRRRITVVAQV 187
            +L    +G DF + +SNV+TW SAALTDE+ C+DGF G+ M G++K+ IR  I  VA+ 
Sbjct: 128 KHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSVAEE 187

Query: 188 TSNALALVNRFADKN 202
           TSNALAL+N FA K+
Sbjct: 188 TSNALALINDFASKH 201

The following BLAST results are available for this feature:

BLAST of Sgr024309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038875547.1	5.6e-80	80.79	21 kDa protein-like [Benincasa hispida]	[more]
XP_022156444.1	4.7e-79	80.86	21 kDa protein-like [Momordica charantia]	[more]
KAA0039678.1	1.4e-78	81.28	21 kDa protein-like [Cucumis melo var. makuwa]	[more]
XP_008437134.1	9.0e-78	80.79	PREDICTED: 21 kDa protein-like [Cucumis melo]	[more]
XP_004147584.1	9.0e-78	79.80	21 kDa protein [Cucumis sativus] >KGN50211.1 hypothetical protein Csa_000459 [Cu...	[more]

BLAST of Sgr024309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SI72	7.9e-45	55.10	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
P17407	1.8e-44	49.25	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9STY5	1.8e-41	47.76	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]
Q9SB37	3.5e-40	47.69	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
Q9SI74	7.7e-32	41.67	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]

BLAST of Sgr024309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DTH0	2.3e-79	80.86	21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111023336 PE=4 SV=1	[more]
A0A5A7T9V3	6.7e-79	81.28	21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold558G...	[more]
A0A0A0KR60	4.3e-78	79.80	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G160140 PE=4 S...	[more]
A0A1S3ATE5	4.3e-78	80.79	21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103482647 PE=4 SV=1	[more]
A0A6J1H2L8	3.2e-73	75.74	pectinesterase inhibitor 9-like OS=Cucurbita moschata OX=3662 GN=LOC111459863 PE...	[more]

BLAST of Sgr024309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G12390.1	2.5e-46	51.27	pectin methylesterase inhibitor 1	[more]
AT1G62770.1	5.6e-46	55.10	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G47380.1	1.3e-42	47.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	8.4e-42	47.74	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	2.5e-41	47.69	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 34..193 e-value: 1.1E-52 score: 191.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 4..195 e-value: 2.7E-36 score: 122.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 37..193 e-value: 1.5E-40 score: 139.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 35..197 e-value: 6.8E-41 score: 141.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 37..197
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 12..201
None	No IPR available	PANTHER	PTHR31080:SF207	PECTINESTERASE INHIBITOR 9	coord: 12..201
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 41..198 e-value: 1.44919E-48 score: 154.133

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr024309.1	Sgr024309.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity