Sgr024276 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr024276
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Descriptionmetalloendoproteinase 1-MMP-like
Locationtig00001291: 1353184 .. 1354382 (+)
RNA-Seq ExpressionSgr024276
SyntenySgr024276
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTTCCGTTTTTCCGTTATGACCTCTTCTTCTACCTTTTCTCCTCTTTCTTCCTCTTCGCCTCTGTTTTTCCCGCCACAACTTCGCCGGACTTTACGACGGCTTTCAACGGAGACGACAACTACGCCTGGAGAAACTTTGCTCGATTCCTCGACGCCGGTAGGGGCAGCGAAGTGACTGGCATGTCGGAGCTGAAGAAGTATTTGAATCGATTCGGTTACCTCCCGAGTGATCCTGAAAATAATTTCACCGATTTCTTCGACGATCAATTCGTATCGGCGTTGCTTCTGTACCAGAAGAGCTTGGGCTTGCCGGTCACCGGAAAGCTCGATTCCAATACAATCTCCAGCATCATGTCGCCGCGGTGTGGTATGAGTGACCTGAGAATTAACGGAACGGCGAAGTTCCACGCGACGCGCCACTACGCGTTTTTTAACGGCCAGCCGAGATGGATTCGCTCCTCGACGTTAACCTACGCTCTGTCACCGGATTACACAATCGATTATCTAACTTCGTCGGAAATTCGGAAGGTCGTCCGACGATCATTCTCGCGGTGGTCGGCAGTGATTCCGTTGAACTTCACCGAGTCCTCGGACTACGAGTCCTCCGACATCCGAATCGGATTCTACCGCGGCGACCACGGAGATGGAGAGCCGTTCGACGGCGTGCTAGGAGTGCTAGCTCACGCGTTCTCGCCGGAAAACGGAAGGCTGCACCTGGACGCGGCGGAGCGGTGGGCGGTGGATTTCAAGAAAGAGAAATCGAAAGTGGCCGTGGATTTGGAATCGGTGGTGTCGCACGAGATAGGGCACGTACTTGGACTCGCTCACTCTTCGGTGAAGGAAGCTGTGATGTATCCGAGTTTGAGTCCGAGGGGGAAGAAGGTGGACCTGAGGATTGATGACGTGGAAGGAGTTCAGGCCTTGTATGGGTCAAACCCTAATTTCAAATTGAAATCATTCTTGGAATCTGAAAAGTCTTTTAACAGTGGAGCACAGAAATTCTCATGGCCCAGTGATTTCTTCTCACTCTTAATTTTTCTATTATTTCTATTTCTATGTGTATGAAATTTGTGATGTAGGTCTTTTTTTTTTTCTTTTTTCATTTTGTTACAAGTGTATATGTATTGAAAGTTACACATCAAACGAAAGGAAAGAAAAGAAAATTGAAAACATCATTCTTATACCTCAAAAAACAC

mRNA sequence

ATGTTTCCGTTTTTCCGTTATGACCTCTTCTTCTACCTTTTCTCCTCTTTCTTCCTCTTCGCCTCTGTTTTTCCCGCCACAACTTCGCCGGACTTTACGACGGCTTTCAACGGAGACGACAACTACGCCTGGAGAAACTTTGCTCGATTCCTCGACGCCGGTAGGGGCAGCGAAGTGACTGGCATGTCGGAGCTGAAGAAGTATTTGAATCGATTCGGTTACCTCCCGAGTGATCCTGAAAATAATTTCACCGATTTCTTCGACGATCAATTCGTATCGGCGTTGCTTCTGTACCAGAAGAGCTTGGGCTTGCCGGTCACCGGAAAGCTCGATTCCAATACAATCTCCAGCATCATGTCGCCGCGGTGTGGTATGAGTGACCTGAGAATTAACGGAACGGCGAAGTTCCACGCGACGCGCCACTACGCGTTTTTTAACGGCCAGCCGAGATGGATTCGCTCCTCGACGTTAACCTACGCTCTGTCACCGGATTACACAATCGATTATCTAACTTCGTCGGAAATTCGGAAGGTCGTCCGACGATCATTCTCGCGGTGGTCGGCAGTGATTCCGTTGAACTTCACCGAGTCCTCGGACTACGAGTCCTCCGACATCCGAATCGGATTCTACCGCGGCGACCACGGAGATGGAGAGCCGTTCGACGGCGTGCTAGGAGTGCTAGCTCACGCGTTCTCGCCGGAAAACGGAAGGCTGCACCTGGACGCGGCGGAGCGGTGGGCGGTGGATTTCAAGAAAGAGAAATCGAAAGTGGCCGTGGATTTGGAATCGGTGGTGTCGCACGAGATAGGGCACGTACTTGGACTCGCTCACTCTTCGGTGAAGGAAGCTGTGATGTATCCGAGTTTGAGTCCGAGGGGGAAGAAGGTGGACCTGAGGATTGATGACGTGGAAGGAGTTCAGGCCTTGTATGGGTCAAACCCTAATTTCAAATTGAAATCATTCTTGGAATCTGAAAAGTCTTTTAACAGTCTTTTTTTTTTTCTTTTTTCATTTTGTTACAAGTGTATATGTATTGAAAGTTACACATCAAACGAAAGGAAAGAAAAGAAAATTGAAAACATCATTCTTATACCTCAAAAAACAC

Coding sequence (CDS)

ATGTTTCCGTTTTTCCGTTATGACCTCTTCTTCTACCTTTTCTCCTCTTTCTTCCTCTTCGCCTCTGTTTTTCCCGCCACAACTTCGCCGGACTTTACGACGGCTTTCAACGGAGACGACAACTACGCCTGGAGAAACTTTGCTCGATTCCTCGACGCCGGTAGGGGCAGCGAAGTGACTGGCATGTCGGAGCTGAAGAAGTATTTGAATCGATTCGGTTACCTCCCGAGTGATCCTGAAAATAATTTCACCGATTTCTTCGACGATCAATTCGTATCGGCGTTGCTTCTGTACCAGAAGAGCTTGGGCTTGCCGGTCACCGGAAAGCTCGATTCCAATACAATCTCCAGCATCATGTCGCCGCGGTGTGGTATGAGTGACCTGAGAATTAACGGAACGGCGAAGTTCCACGCGACGCGCCACTACGCGTTTTTTAACGGCCAGCCGAGATGGATTCGCTCCTCGACGTTAACCTACGCTCTGTCACCGGATTACACAATCGATTATCTAACTTCGTCGGAAATTCGGAAGGTCGTCCGACGATCATTCTCGCGGTGGTCGGCAGTGATTCCGTTGAACTTCACCGAGTCCTCGGACTACGAGTCCTCCGACATCCGAATCGGATTCTACCGCGGCGACCACGGAGATGGAGAGCCGTTCGACGGCGTGCTAGGAGTGCTAGCTCACGCGTTCTCGCCGGAAAACGGAAGGCTGCACCTGGACGCGGCGGAGCGGTGGGCGGTGGATTTCAAGAAAGAGAAATCGAAAGTGGCCGTGGATTTGGAATCGGTGGTGTCGCACGAGATAGGGCACGTACTTGGACTCGCTCACTCTTCGGTGAAGGAAGCTGTGATGTATCCGAGTTTGAGTCCGAGGGGGAAGAAGGTGGACCTGAGGATTGATGACGTGGAAGGAGTTCAGGCCTTGTATGGGTCAAACCCTAATTTCAAATTGAAATCATTCTTGGAATCTGAAAAGTCTTTTAACAGTCTTTTTTTTTTTCTTTTTTCATTTTGTTACAAGTGTATATGTATTGAAAGTTACACATCAAACGAAAGGAAAGAAAAGAAAATTGAAAACATCATTCTTATACCTCAAAAAACAC

Protein sequence

MFPFFRYDLFFYLFSSFFLFASVFPATTSPDFTTAFNGDDNYAWRNFARFLDAGRGSEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISSIMSPRCGMSDLRINGTAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFNSLFFFLFSFCYKCICIESYTSNERKEKKIENIILIPQKTX
Homology
BLAST of Sgr024276 vs. NCBI nr
Match: XP_023532756.1 (metalloendoproteinase 4-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 566.6 bits (1459), Expect = 1.6e-157
Identity = 295/353 (83.57%), Postives = 313/353 (88.67%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRG 60
           MFPFFRY L FFYL SS FLF+SVFPATT  DFTT   AF+  +NYAWRNFARFLDAG G
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYAWRNFARFLDAGEG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLP  PENNF+DF DDQFVSAL+LYQK LGL VTGKLDS TI+
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDL-RING--TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SIMSPRCGMSDL +IN   T+  H+TR YAFFNGQPRWIR+STLTYALSPDYTI+YLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFNS--------LFFFLFSF 339
           KKV+LR+DDVEGVQALYGSNPNFKL SFLESEKS N+         FFF FSF
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFFFFFFSF 353

BLAST of Sgr024276 vs. NCBI nr
Match: KAG7036120.1 (Metalloendoproteinase 1-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.3 bits (1453), Expect = 7.7e-157
Identity = 295/361 (81.72%), Postives = 314/361 (86.98%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRG 60
           MFPFFRY L FFYL SS FLF+SVFPATT  DFTT   AF+  +NY WRNFARFLDAG+G
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDVSNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLP  PENNF+DF DDQFVSAL+LYQK LGL VTGKLDS TI+
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDL-RING--TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SIMSPRCGMSDL +IN   T+  H+TR YAFFNGQPRWIR STLTYALSPDYTI+YLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFN----------SLFFFLFSFCYKCI 345
           KKV+LR+DDVEGVQALYGSNPNFKL SFLESEKS N          S FFF F F +  +
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFSFFFFSFLFFFIFV 360

BLAST of Sgr024276 vs. NCBI nr
Match: XP_022957642.1 (metalloendoproteinase 4-MMP-like [Cucurbita moschata])

HSP 1 Score: 562.8 bits (1449), Expect = 2.2e-156
Identity = 294/358 (82.12%), Postives = 312/358 (87.15%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRG 60
           MFPFFRY L FFYL SS FLF+SVFPATT  DFTT   AF+  +NY WRNFARFLDAG+G
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLP  PENNF+DF DDQFVSAL+LYQK LGL VTGKLDS TI+
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDL-RING--TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SIMSPRCGMSDL +IN   T+  H+TR YAFFNGQPRWIR STLTYALSPDYTI+YLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFN---------------SLFFFLF 337
           KKV+LR+DDVEGVQALYGSNPNFKL SFLESEKS N               SLFFF+F
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFSFFFFSLFFFIF 358

BLAST of Sgr024276 vs. NCBI nr
Match: XP_022996404.1 (metalloendoproteinase 1-MMP-like [Cucurbita maxima])

HSP 1 Score: 558.5 bits (1438), Expect = 4.2e-155
Identity = 291/353 (82.44%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRG 60
           MFPFFRY + FFYL SS FLF+SVFPATT  DFTT   AF+  +NY WRNFARFLDAG+G
Sbjct: 1   MFPFFRYYIFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLP  PENNF+DF DDQFVSAL+LYQ+ LGL VTGKLDS TI+
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVFPENNFSDFVDDQFVSALILYQRRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDL-RING--TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SIMSPRCGMSDL +IN   T   H+TR YAFFNGQPRWIR STLTYALSPDYTI+YLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTTTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIR VV RSFSRWSAVIPLNFTESS+YESSDIRIGFY GDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRNVVGRSFSRWSAVIPLNFTESSNYESSDIRIGFYHGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFN----------SLFFFLF 337
           KKV+LR+DDVEGVQALYGSNPNFKL SFLESEKS N          SLFFF+F
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFLSLFFFIF 353

BLAST of Sgr024276 vs. NCBI nr
Match: XP_038875312.1 (metalloendoproteinase 1-MMP [Benincasa hispida])

HSP 1 Score: 556.6 bits (1433), Expect = 1.6e-154
Identity = 285/353 (80.74%), Postives = 309/353 (87.54%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTTAFNGDDNYAWRNFARFLDAGRGSEV 60
           M PFFRY L FFY+FSS FLF+ VFPA T PDFTT  + D+NY WRNFARFLDAG+G+EV
Sbjct: 1   MLPFFRYSLFFFYIFSSVFLFSPVFPARTLPDFTT-LDADNNYGWRNFARFLDAGKGTEV 60

Query: 61  TGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISSIM 120
            GMSELKKYLNRFGYLP  P+NNF+DFFDDQF+SAL+LYQ  LGL VTGKLDS TI+SI+
Sbjct: 61  NGMSELKKYLNRFGYLPIPPQNNFSDFFDDQFLSALILYQNRLGLSVTGKLDSETIASII 120

Query: 121 SPRCGMSDL-RING-----TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SPRCGMSDL +IN      T   H+TR YAFFNGQPRWIRSSTLTYALSPDYTI+YLT S
Sbjct: 121 SPRCGMSDLIKINNNTTTTTTTIHSTRRYAFFNGQPRWIRSSTLTYALSPDYTIEYLTPS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIRKV+RRSFSRWSAVIPLNFTESSDYESSDIRIGF+RGDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRKVIRRSFSRWSAVIPLNFTESSDYESSDIRIGFFRGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKE+VMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSTVKESVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFNS--------LFFFLFSF 339
           KKVDLRIDDVEG+Q LYG+NPNFKLKSFLESEKS +S         FFFL  F
Sbjct: 301 KKVDLRIDDVEGIQVLYGTNPNFKLKSFLESEKSIDSGSSSSNDANFFFLLVF 352

BLAST of Sgr024276 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 343.6 bits (880), Expect = 2.8e-93
Identity = 167/292 (57.19%), Postives = 217/292 (74.32%), Query Frame = 0

Query: 41  NYAWRNFARFLDAGRGSEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQK 100
           N  W +F+R +D   GS V+G+SELK+YL+RFGY+ +D    F+D FD    SA+ LYQ+
Sbjct: 49  NSTWHDFSRLVDVQIGSHVSGVSELKRYLHRFGYV-NDGSEIFSDVFDGPLESAISLYQE 108

Query: 101 SLGLPVTGKLDSNTISSIMSPRCGMSDLRINGTAKF-HATRHYAFFNGQPRWIRSSTLTY 160
           +LGLP+TG+LD++T++ +  PRCG+SD  +     F H T HY +FNG+P+W R  TLTY
Sbjct: 109 NLGLPITGRLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKWNR-DTLTY 168

Query: 161 ALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEP 220
           A+S  + +DYLTS +++ V RR+FS+WS+VIP++F E  D+ ++D++IGFY GDHGDG P
Sbjct: 169 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 228

Query: 221 FDGVLGVLAHAFSPENGRLHLDAAERWAVDFK-KEKSKVAVDLESVVSHEIGHVLGLAHS 280
           FDGVLG LAHAF+PENGRLHLDAAE W VD   K  S+VAVDLESV +HEIGH+LGL HS
Sbjct: 229 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 288

Query: 281 SVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFNS 331
           S + AVMYPSL PR KKVDL +DDV GV  LYG NP  +L S  +SE S  +
Sbjct: 289 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPNPKLRLDSLTQSEDSIKN 338

BLAST of Sgr024276 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 1.1e-89
Identity = 171/314 (54.46%), Postives = 216/314 (68.79%), Query Frame = 0

Query: 25  PATTSPDFTTAFNGDDNYAWRNFARFLDAGRGSEVT-------GMSELKKYLNRFGYLPS 84
           P    P FTT F+    Y   +  + ++A   S+ T        + E+K++L ++GYLP 
Sbjct: 7   PCNRKP-FTTIFSFFLLYLNLHNQQIIEARNPSQFTTNPSPDVSIPEIKRHLQQYGYLPQ 66

Query: 85  DPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISSIMSPRCGMSDLRINGTAKFH 144
           + E++     D  F  AL+ YQK+LGLP+TGK DS+T+S I+ PRCG  D     TA FH
Sbjct: 67  NKESD-----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDVEPKTAPFH 126

Query: 145 ATRHYAFFNGQPRWIRS--STLTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFT 204
             + Y +F G+PRW R     LTYA S +    YL  ++IR+V RR+F +W++VIP++F 
Sbjct: 127 TGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFI 186

Query: 205 ESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKS 264
           E+ DY  +DI+IGF+ GDHGDGEPFDGVLGVLAH FSPENGRLHLD AE WAVDF +EKS
Sbjct: 187 ETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKS 246

Query: 265 KVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPN 324
            VAVDLESV  HEIGHVLGL HSSVK+A MYP+L PR KKV+L +DDV GVQ+LYG+NPN
Sbjct: 247 SVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTNPN 306

Query: 325 FKLKSFLESEKSFN 330
           F L S L SE S N
Sbjct: 307 FTLNSLLASETSTN 314

BLAST of Sgr024276 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.9e-73
Identity = 153/327 (46.79%), Postives = 191/327 (58.41%), Query Frame = 0

Query: 14  FSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRGSEVTGMSELKKYLN 73
           F S FL  S   A   P+ T    +        W  F+ F     G  V G+  +KKY  
Sbjct: 8   FLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQ 67

Query: 74  RFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISSIMSPRCGMSDLRI 133
           RFGY+P     NFTD FDD   +A+ LYQ +  L VTG+LD+ TI  I+ PRCG  D+ +
Sbjct: 68  RFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDV-V 127

Query: 134 NGTA-----------------KFHATRHYAFFNGQPRWIRS-STLTYALSPDYTIDYLTS 193
           NGT+                   HA + Y  F G+PRW R+   LTYA  P   +    +
Sbjct: 128 NGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPL----T 187

Query: 194 SEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFS 253
            E++ V  R+F RWS V  LNFT S  + +SDI IGFY GDHGDGEPFDGVLG LAHAFS
Sbjct: 188 EEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFS 247

Query: 254 PENGRLHLDAAERWAVDFKKE---KSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSL 313
           P +G+ HLDA E W V    +       AVDLESV  HEIGH+LGL HSSV+E++MYP++
Sbjct: 248 PPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTI 307

Query: 314 SPRGKKVDLRIDDVEGVQALYGSNPNF 317
           +   +KVDL  DDVEG+Q LYG+NPNF
Sbjct: 308 TTGKRKVDLTNDDVEGIQYLYGANPNF 329

BLAST of Sgr024276 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.3e-69
Identity = 134/284 (47.18%), Postives = 178/284 (62.68%), Query Frame = 0

Query: 43  AWRNFARFLDAGRGSEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSL 102
           AW  F++      G  + G+S+LK+Y  RFGY+ +    N TD FDD   SA+  YQK+ 
Sbjct: 41  AWETFSKLAGCHIGENINGLSKLKQYFRRFGYITT--TGNCTDDFDDVLQSAINTYQKNF 100

Query: 103 GLPVTGKLDSNTISSIMSPRCGMSDL-----RINGTAKFHATRHYAFFNGQPRW-IRSST 162
            L VTGKLDS+T+  I+ PRCG  DL      +NG      T  Y+FF G+PRW  R   
Sbjct: 101 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRD 160

Query: 163 LTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGD 222
           LTYA +P   +    + E+++V  R+F+RW+ V PLNFT S     +DI IGF+ G+HGD
Sbjct: 161 LTYAFAPQNNL----TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGD 220

Query: 223 GEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKSKV-----AVDLESVVSHEIGH 282
           GEPFDG +G LAHA SP  G LHLD  E W +   +   ++      VDLESV  HEIGH
Sbjct: 221 GEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGH 280

Query: 283 VLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPN 316
           +LGL HSSV++A+M+P++S   +KV+L  DD+EG+Q LYG NPN
Sbjct: 281 LLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGGNPN 318

BLAST of Sgr024276 vs. ExPASy Swiss-Prot
Match: Q5XF51 (Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 1.2e-67
Identity = 135/297 (45.45%), Postives = 178/297 (59.93%), Query Frame = 0

Query: 44  WRNFARFLDAGRGSEVTGMSELKKYLNRFGYL-PSDPENNFTDFFDDQFVSALLLYQKSL 103
           W +F  F     G +  G+  LK+Y   FGY+  ++   NFTD FDD   +A+ +YQ++ 
Sbjct: 43  WNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNF 102

Query: 104 GLPVTGKLDSNTISSIMSPRCGMSDLRINGTA------------------KFHATRHYAF 163
            L VTG LD  T+  ++ PRCG  D+ +NGT+                  +FHA +HY+F
Sbjct: 103 QLNVTGVLDELTLKHVVIPRCGNPDV-VNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSF 162

Query: 164 FNGQPRWIRS-STLTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESS 223
           F G+PRW R+   LTYA  P   +    + E++ V  R+F+RW  V PL FT    + +S
Sbjct: 163 FPGEPRWPRNRRDLTYAFDPRNAL----TEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 222

Query: 224 DIRIGFYRGDHGDGEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKSKV----AV 283
           DI IGFY G+HGDGEPFDG +  LAHAFSP  G  HLD  E W V  +     +    AV
Sbjct: 223 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 282

Query: 284 DLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPNF 317
           DLESV  HEIGH+LGL HSSV+ ++MYP++    +KVDL  DDVEGVQ LYG+NPNF
Sbjct: 283 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGANPNF 334

BLAST of Sgr024276 vs. ExPASy TrEMBL
Match: A0A6J1GZP3 (metalloendoproteinase 4-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111459118 PE=3 SV=1)

HSP 1 Score: 562.8 bits (1449), Expect = 1.1e-156
Identity = 294/358 (82.12%), Postives = 312/358 (87.15%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRG 60
           MFPFFRY L FFYL SS FLF+SVFPATT  DFTT   AF+  +NY WRNFARFLDAG+G
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLP  PENNF+DF DDQFVSAL+LYQK LGL VTGKLDS TI+
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDL-RING--TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SIMSPRCGMSDL +IN   T+  H+TR YAFFNGQPRWIR STLTYALSPDYTI+YLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFN---------------SLFFFLF 337
           KKV+LR+DDVEGVQALYGSNPNFKL SFLESEKS N               SLFFF+F
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFSFFFFSLFFFIF 358

BLAST of Sgr024276 vs. ExPASy TrEMBL
Match: A0A6J1K8M4 (metalloendoproteinase 1-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111491637 PE=3 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 2.0e-155
Identity = 291/353 (82.44%), Postives = 310/353 (87.82%), Query Frame = 0

Query: 1   MFPFFRYDL-FFYLFSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRG 60
           MFPFFRY + FFYL SS FLF+SVFPATT  DFTT   AF+  +NY WRNFARFLDAG+G
Sbjct: 1   MFPFFRYYIFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLP  PENNF+DF DDQFVSAL+LYQ+ LGL VTGKLDS TI+
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVFPENNFSDFVDDQFVSALILYQRRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDL-RING--TAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSS 180
           SIMSPRCGMSDL +IN   T   H+TR YAFFNGQPRWIR STLTYALSPDYTI+YLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTTTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSP 240
           EIR VV RSFSRWSAVIPLNFTESS+YESSDIRIGFY GDHGDGE FDGVLGVLAHAFSP
Sbjct: 181 EIRNVVGRSFSRWSAVIPLNFTESSNYESSDIRIGFYHGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFN----------SLFFFLF 337
           KKV+LR+DDVEGVQALYGSNPNFKL SFLESEKS N          SLFFF+F
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFLSLFFFIF 353

BLAST of Sgr024276 vs. ExPASy TrEMBL
Match: A0A6J1I297 (metalloendoproteinase 1-MMP OS=Cucurbita maxima OX=3661 GN=LOC111468956 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.3e-153
Identity = 288/355 (81.13%), Postives = 305/355 (85.92%), Query Frame = 0

Query: 1   MFPFFRYDLFFYLFSSFFLFASVFPATTSPDFTTA---FNGDDNYAWRNFARFLDAGRGS 60
           MFPFFRY L F     F+L  SVFPA T  DFT A    +GD+NYAWRNFARFLDAG+G 
Sbjct: 1   MFPFFRYYLCF-----FYLLYSVFPARTFADFTAATASLDGDNNYAWRNFARFLDAGKGR 60

Query: 61  EVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISS 120
           EV GMSELKKYLNRFGYLP  PENNF+D FDDQFVSAL+LYQK LGL VTGKLDS TI+S
Sbjct: 61  EVNGMSELKKYLNRFGYLPIPPENNFSDSFDDQFVSALILYQKRLGLSVTGKLDSETIAS 120

Query: 121 IMSPRCGMSDL-RINGTAK--FHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSSE 180
           IMSPRCGMSDL +IN +      +TR YAFFNGQPRWIRSSTLTYALSPDYTI+YLTSSE
Sbjct: 121 IMSPRCGMSDLIKINHSVSTTIRSTRRYAFFNGQPRWIRSSTLTYALSPDYTIEYLTSSE 180

Query: 181 IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSPE 240
           IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGF+RGDHGDGE FDGVLG+LAHAFSPE
Sbjct: 181 IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFFRGDHGDGESFDGVLGILAHAFSPE 240

Query: 241 NGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGK 300
           NGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRGK
Sbjct: 241 NGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGK 300

Query: 301 KVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFN-------------SLFFFLF 337
           KVDLRIDDVEGVQALYGSNPNFKLKSFLESEKS N             + FFFLF
Sbjct: 301 KVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSINNNSGSSSSSSNNANFFFFLF 350

BLAST of Sgr024276 vs. ExPASy TrEMBL
Match: A0A6J1E2L9 (metalloendoproteinase 1-MMP OS=Cucurbita moschata OX=3662 GN=LOC111430227 PE=3 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.2e-152
Identity = 288/355 (81.13%), Postives = 305/355 (85.92%), Query Frame = 0

Query: 1   MFPFFRYDLFFYLFSSFFLFASVFPATTSPDFTTA---FNGDDNYAWRNFARFLDAGRGS 60
           MFPFFRY L F     F+L  SVFPATT  DFT A    +GD+NYAWRNFARFLDAG+G 
Sbjct: 1   MFPFFRYYLCF-----FYLLYSVFPATTFADFTAATASLDGDNNYAWRNFARFLDAGKGR 60

Query: 61  EVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISS 120
           EV GMSELKKYLNRFGYLP  PENNF+D FDDQFVSAL+LYQK LGL VTGKLDS TI+S
Sbjct: 61  EVNGMSELKKYLNRFGYLPIPPENNFSDSFDDQFVSALILYQKRLGLSVTGKLDSETIAS 120

Query: 121 IMSPRCGMSDL-RINGTAK--FHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSSE 180
           IMSPRCGMSDL +IN +      +TR YAFFNGQPRWIRSSTLTYALSPDYTI+YLTSSE
Sbjct: 121 IMSPRCGMSDLIKINHSVSTTIRSTRRYAFFNGQPRWIRSSTLTYALSPDYTIEYLTSSE 180

Query: 181 IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSPE 240
           IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGF+RGDHGDGE FDGVLG+LAHAFSPE
Sbjct: 181 IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFFRGDHGDGESFDGVLGILAHAFSPE 240

Query: 241 NGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGK 300
           NGRLHLDAAERWAVDF+KEKSKVAVDLESVV+HEIGHVLGLAHS+VKEAVMYPSLSPRGK
Sbjct: 241 NGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGK 300

Query: 301 KVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSF-----------NSLFFFLFSF 339
           KVDLRIDDVEGVQALYGSNPNFKLKS LESEKS            N+ FFFL  F
Sbjct: 301 KVDLRIDDVEGVQALYGSNPNFKLKSLLESEKSINNNSGSSSSSNNANFFFLLFF 350

BLAST of Sgr024276 vs. ExPASy TrEMBL
Match: A0A6J1DLC4 (metalloendoproteinase 1-MMP OS=Momordica charantia OX=3673 GN=LOC111021985 PE=3 SV=1)

HSP 1 Score: 548.1 bits (1411), Expect = 2.8e-152
Identity = 287/353 (81.30%), Postives = 309/353 (87.54%), Query Frame = 0

Query: 1   MFPFFRYDLFFYLFSSFFLFASVFPATTSPDFTTA-FNGDD---NYAWRNFARFLDAGRG 60
           MFPFFRY    YLF S F FASVFPATTSPD   A F+G D   ++AWRNFARFLDAG+G
Sbjct: 1   MFPFFRY----YLFFSLFFFASVFPATTSPDSMAANFDGGDGHNDFAWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTIS 120
           SEVTGMSELKKYLNRFGYLPS  + +FTDFFDDQFVSAL+LYQK LGLPVTG LDS+TIS
Sbjct: 61  SEVTGMSELKKYLNRFGYLPSS-DTDFTDFFDDQFVSALILYQKRLGLPVTGNLDSDTIS 120

Query: 121 SIMSPRCGMSD-LRIN-GTAKFHATRHYAFFNGQPRWIRSSTLTYALSPDYTIDYLTSSE 180
           SI+SPRCGMSD ++IN  TA+ H+TR YAFFNGQPRWIRSSTLTYALSPDYT+DYL+SSE
Sbjct: 121 SIISPRCGMSDVVKINRSTAELHSTRRYAFFNGQPRWIRSSTLTYALSPDYTVDYLSSSE 180

Query: 181 IRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSPE 240
           IR+VVRRSFSRWSAVIP+NFTES +YESSDIRIGFYRG+HGDGE FDGVLGVLAHAFSPE
Sbjct: 181 IRRVVRRSFSRWSAVIPVNFTESREYESSDIRIGFYRGEHGDGEAFDGVLGVLAHAFSPE 240

Query: 241 NGRLHLDAAERWAVDFKKEKSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGK 300
           NGRLHLDAAERWAVD +KEKSKVAVDLESVV+HEIGHVLGLAHSSVKEAVMYPSLSPR K
Sbjct: 241 NGRLHLDAAERWAVDLEKEKSKVAVDLESVVTHEIGHVLGLAHSSVKEAVMYPSLSPRAK 300

Query: 301 KVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFNS----------LFFFLFS 338
           KVDLRIDDVEG QALYGSNPNFKLKSFLESE S N+           F FLFS
Sbjct: 301 KVDLRIDDVEGAQALYGSNPNFKLKSFLESENSINTATRLRNFSSPFFIFLFS 348

BLAST of Sgr024276 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 343.6 bits (880), Expect = 2.0e-94
Identity = 167/292 (57.19%), Postives = 217/292 (74.32%), Query Frame = 0

Query: 41  NYAWRNFARFLDAGRGSEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQK 100
           N  W +F+R +D   GS V+G+SELK+YL+RFGY+ +D    F+D FD    SA+ LYQ+
Sbjct: 49  NSTWHDFSRLVDVQIGSHVSGVSELKRYLHRFGYV-NDGSEIFSDVFDGPLESAISLYQE 108

Query: 101 SLGLPVTGKLDSNTISSIMSPRCGMSDLRINGTAKF-HATRHYAFFNGQPRWIRSSTLTY 160
           +LGLP+TG+LD++T++ +  PRCG+SD  +     F H T HY +FNG+P+W R  TLTY
Sbjct: 109 NLGLPITGRLDTSTVTLMSLPRCGVSDTHMTINNDFLHTTAHYTYFNGKPKWNR-DTLTY 168

Query: 161 ALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEP 220
           A+S  + +DYLTS +++ V RR+FS+WS+VIP++F E  D+ ++D++IGFY GDHGDG P
Sbjct: 169 AISKTHKLDYLTSEDVKTVFRRAFSQWSSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLP 228

Query: 221 FDGVLGVLAHAFSPENGRLHLDAAERWAVDFK-KEKSKVAVDLESVVSHEIGHVLGLAHS 280
           FDGVLG LAHAF+PENGRLHLDAAE W VD   K  S+VAVDLESV +HEIGH+LGL HS
Sbjct: 229 FDGVLGTLAHAFAPENGRLHLDAAETWIVDDDLKGSSEVAVDLESVATHEIGHLLGLGHS 288

Query: 281 SVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSFNS 331
           S + AVMYPSL PR KKVDL +DDV GV  LYG NP  +L S  +SE S  +
Sbjct: 289 SQESAVMYPSLRPRTKKVDLTVDDVAGVLKLYGPNPKLRLDSLTQSEDSIKN 338

BLAST of Sgr024276 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 331.6 bits (849), Expect = 7.8e-91
Identity = 171/314 (54.46%), Postives = 216/314 (68.79%), Query Frame = 0

Query: 25  PATTSPDFTTAFNGDDNYAWRNFARFLDAGRGSEVT-------GMSELKKYLNRFGYLPS 84
           P    P FTT F+    Y   +  + ++A   S+ T        + E+K++L ++GYLP 
Sbjct: 7   PCNRKP-FTTIFSFFLLYLNLHNQQIIEARNPSQFTTNPSPDVSIPEIKRHLQQYGYLPQ 66

Query: 85  DPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISSIMSPRCGMSDLRINGTAKFH 144
           + E++     D  F  AL+ YQK+LGLP+TGK DS+T+S I+ PRCG  D     TA FH
Sbjct: 67  NKESD-----DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPDDVEPKTAPFH 126

Query: 145 ATRHYAFFNGQPRWIRS--STLTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFT 204
             + Y +F G+PRW R     LTYA S +    YL  ++IR+V RR+F +W++VIP++F 
Sbjct: 127 TGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRAFGKWASVIPVSFI 186

Query: 205 ESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKS 264
           E+ DY  +DI+IGF+ GDHGDGEPFDGVLGVLAH FSPENGRLHLD AE WAVDF +EKS
Sbjct: 187 ETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDKAETWAVDFDEEKS 246

Query: 265 KVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPN 324
            VAVDLESV  HEIGHVLGL HSSVK+A MYP+L PR KKV+L +DDV GVQ+LYG+NPN
Sbjct: 247 SVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDDVVGVQSLYGTNPN 306

Query: 325 FKLKSFLESEKSFN 330
           F L S L SE S N
Sbjct: 307 FTLNSLLASETSTN 314

BLAST of Sgr024276 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 277.7 bits (709), Expect = 1.3e-74
Identity = 153/327 (46.79%), Postives = 191/327 (58.41%), Query Frame = 0

Query: 14  FSSFFLFASVFPATTSPDFTT---AFNGDDNYAWRNFARFLDAGRGSEVTGMSELKKYLN 73
           F S FL  S   A   P+ T    +        W  F+ F     G  V G+  +KKY  
Sbjct: 8   FLSLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDGLYRIKKYFQ 67

Query: 74  RFGYLPSDPENNFTDFFDDQFVSALLLYQKSLGLPVTGKLDSNTISSIMSPRCGMSDLRI 133
           RFGY+P     NFTD FDD   +A+ LYQ +  L VTG+LD+ TI  I+ PRCG  D+ +
Sbjct: 68  RFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIPRCGNPDV-V 127

Query: 134 NGTA-----------------KFHATRHYAFFNGQPRWIRS-STLTYALSPDYTIDYLTS 193
           NGT+                   HA + Y  F G+PRW R+   LTYA  P   +    +
Sbjct: 128 NGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPKNPL----T 187

Query: 194 SEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEPFDGVLGVLAHAFS 253
            E++ V  R+F RWS V  LNFT S  + +SDI IGFY GDHGDGEPFDGVLG LAHAFS
Sbjct: 188 EEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVLGTLAHAFS 247

Query: 254 PENGRLHLDAAERWAVDFKKE---KSKVAVDLESVVSHEIGHVLGLAHSSVKEAVMYPSL 313
           P +G+ HLDA E W V    +       AVDLESV  HEIGH+LGL HSSV+E++MYP++
Sbjct: 248 PPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVEESIMYPTI 307

Query: 314 SPRGKKVDLRIDDVEGVQALYGSNPNF 317
           +   +KVDL  DDVEG+Q LYG+NPNF
Sbjct: 308 TTGKRKVDLTNDDVEGIQYLYGANPNF 329

BLAST of Sgr024276 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 265.0 bits (676), Expect = 9.0e-71
Identity = 134/284 (47.18%), Postives = 178/284 (62.68%), Query Frame = 0

Query: 43  AWRNFARFLDAGRGSEVTGMSELKKYLNRFGYLPSDPENNFTDFFDDQFVSALLLYQKSL 102
           AW  F++      G  + G+S+LK+Y  RFGY+ +    N TD FDD   SA+  YQK+ 
Sbjct: 41  AWETFSKLAGCHIGENINGLSKLKQYFRRFGYITT--TGNCTDDFDDVLQSAINTYQKNF 100

Query: 103 GLPVTGKLDSNTISSIMSPRCGMSDL-----RINGTAKFHATRHYAFFNGQPRW-IRSST 162
            L VTGKLDS+T+  I+ PRCG  DL      +NG      T  Y+FF G+PRW  R   
Sbjct: 101 NLKVTGKLDSSTLRQIVKPRCGNPDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRD 160

Query: 163 LTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGD 222
           LTYA +P   +    + E+++V  R+F+RW+ V PLNFT S     +DI IGF+ G+HGD
Sbjct: 161 LTYAFAPQNNL----TDEVKRVFSRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGD 220

Query: 223 GEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKSKV-----AVDLESVVSHEIGH 282
           GEPFDG +G LAHA SP  G LHLD  E W +   +   ++      VDLESV  HEIGH
Sbjct: 221 GEPFDGAMGTLAHASSPPTGMLHLDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGH 280

Query: 283 VLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPN 316
           +LGL HSSV++A+M+P++S   +KV+L  DD+EG+Q LYG NPN
Sbjct: 281 LLGLGHSSVEDAIMFPAISGGDRKVELAKDDIEGIQHLYGGNPN 318

BLAST of Sgr024276 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 258.5 bits (659), Expect = 8.4e-69
Identity = 135/297 (45.45%), Postives = 178/297 (59.93%), Query Frame = 0

Query: 44  WRNFARFLDAGRGSEVTGMSELKKYLNRFGYL-PSDPENNFTDFFDDQFVSALLLYQKSL 103
           W +F  F     G +  G+  LK+Y   FGY+  ++   NFTD FDD   +A+ +YQ++ 
Sbjct: 43  WNSFLNFTGCHAGKKYDGLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNF 102

Query: 104 GLPVTGKLDSNTISSIMSPRCGMSDLRINGTA------------------KFHATRHYAF 163
            L VTG LD  T+  ++ PRCG  D+ +NGT+                  +FHA +HY+F
Sbjct: 103 QLNVTGVLDELTLKHVVIPRCGNPDV-VNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSF 162

Query: 164 FNGQPRWIRS-STLTYALSPDYTIDYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESS 223
           F G+PRW R+   LTYA  P   +    + E++ V  R+F+RW  V PL FT    + +S
Sbjct: 163 FPGEPRWPRNRRDLTYAFDPRNAL----TEEVKSVFSRAFTRWEEVTPLTFTRVERFSTS 222

Query: 224 DIRIGFYRGDHGDGEPFDGVLGVLAHAFSPENGRLHLDAAERWAVDFKKEKSKV----AV 283
           DI IGFY G+HGDGEPFDG +  LAHAFSP  G  HLD  E W V  +     +    AV
Sbjct: 223 DISIGFYSGEHGDGEPFDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAV 282

Query: 284 DLESVVSHEIGHVLGLAHSSVKEAVMYPSLSPRGKKVDLRIDDVEGVQALYGSNPNF 317
           DLESV  HEIGH+LGL HSSV+ ++MYP++    +KVDL  DDVEGVQ LYG+NPNF
Sbjct: 283 DLESVAVHEIGHLLGLGHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGANPNF 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023532756.11.6e-15783.57metalloendoproteinase 4-MMP-like [Cucurbita pepo subsp. pepo][more]
KAG7036120.17.7e-15781.72Metalloendoproteinase 1-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022957642.12.2e-15682.12metalloendoproteinase 4-MMP-like [Cucurbita moschata][more]
XP_022996404.14.2e-15582.44metalloendoproteinase 1-MMP-like [Cucurbita maxima][more]
XP_038875312.11.6e-15480.74metalloendoproteinase 1-MMP [Benincasa hispida][more]
Match NameE-valueIdentityDescription
O235072.8e-9357.19Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Q8GWW61.1e-8954.46Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1[more]
O045291.9e-7346.79Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ51.3e-6947.18Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
Q5XF511.2e-6745.45Metalloendoproteinase 3-MMP OS=Arabidopsis thaliana OX=3702 GN=3MMP PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GZP31.1e-15682.12metalloendoproteinase 4-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111459118 P... [more]
A0A6J1K8M42.0e-15582.44metalloendoproteinase 1-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111491637 PE=... [more]
A0A6J1I2974.3e-15381.13metalloendoproteinase 1-MMP OS=Cucurbita maxima OX=3661 GN=LOC111468956 PE=3 SV=... [more]
A0A6J1E2L91.2e-15281.13metalloendoproteinase 1-MMP OS=Cucurbita moschata OX=3662 GN=LOC111430227 PE=3 S... [more]
A0A6J1DLC42.8e-15281.30metalloendoproteinase 1-MMP OS=Momordica charantia OX=3673 GN=LOC111021985 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT4G16640.12.0e-9457.19Matrixin family protein [more]
AT2G45040.17.8e-9154.46Matrixin family protein [more]
AT1G70170.11.3e-7446.79matrix metalloproteinase [more]
AT1G59970.19.0e-7147.18Matrixin family protein [more]
AT1G24140.18.4e-6945.45Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 203..231
score: 59.88
coord: 118..131
score: 36.67
coord: 298..311
score: 58.8
coord: 264..289
score: 54.13
coord: 179..194
score: 44.82
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 147..312
e-value: 3.6E-43
score: 159.4
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 51..317
e-value: 3.0E-79
score: 267.9
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 150..311
e-value: 6.7E-50
score: 169.0
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 62..116
e-value: 2.8E-11
score: 43.4
NoneNo IPR availablePIRSRPIRSR001191-1PIRSR001191-1coord: 61..324
e-value: 1.8E-65
score: 219.3
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 34..330
NoneNo IPR availablePANTHERPTHR10201:SF245METALLOENDOPROTEINASE 4-MMPcoord: 34..330
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 148..314
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 150..311
e-value: 1.7616E-67
score: 208.212
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 53..128

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr024276.1Sgr024276.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity