Sgr024142 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr024142
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Cytochrome P450
Location	tig00001047: 3594837 .. 3596361 (-)
RNA-Seq Expression	Sgr024142
Synteny	Sgr024142

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGACAAAAAAACCAGTGGAAGATCATCACTTGTAAACGCCATGGAAGTTCTGCAAACTTCAGCCCCATCATCATAGCTTTGAGCTTGGCAATGGCGGCAATCATCTGCCGCTCTCTTCTGAAACTCTTTGCAACAGCAGCGCCAAACCCTAATTTGCCTCCCGGCTGCATGGGCTTCCCCATCGTCGGAGAGACTCTCGGCTTCCTCAAGCCTCACGATTCAAACTCCATTGGAACCTTCCTGCACGACCACTGCTCCAGGTAAAAGATACACACACACAAGAAAAAAAAAGGAGATCGAAGTGAAAACTATAAAATGTGTTTTTTTTTTTTTTGTTCTGTTAGGTTCGGGAAGGTTTTCAGATCCCATCTATTCGGTTCGCCGGCGATAGTGTCGTGCGATCGAGAGCTTAATATGTTCATTCTTCAGAACGACGACAAGCTTTTCAAAGTGAGCTATCCGAAGGCCATGCATGGCATTCTCGGAAGCAATTCGTTGATCATTGCCACCGGAGACACCCACCGGAAGCTGAGAAACGTCGTCGTTAGCTTCATCAGCTGGTGCAAGTCCAGACCTAGTTTCCTTGAGTGCGTGGAGAAGCTGGTTCTCTCTTTAATGGAGTCCTGGAGATGCCAAAACCAAGTGCTTTTCTTCAAAGAAGTGAAGATGGTAAAATTACTCAGAAATTATTATATTATCATTAATTTTTGCATATAAAAATGGGAGGAATAATTTAATCGATATGTCGTTTGCTATGTTATTTTTCAGTTTGCTCTGAGCGTTGTGGTGAAGGAACTGCTGGGCATCGAACCAGACGAAGCTATAGGGTCAAAGATATTGGAAGAGTTTGAAACGTATATGAAAGGCTTTGTTTCTTTGCCTCTAAATCTCCTGGAACTCCATACTCTAAGGCTGTCAAGGTCCTATTCTCTCCCTCTCTGATCTCTGTAGATTGCATGGATTATATTATTATTATTATGTTGTTGTTGTTGATCAGGCTAGAGGGAGGCTTTCTTGCATTGTGAAAGAGATGATGAGAGAGAGGAGGATGAGGGGATTATTGGTGGGGGTGGAAATAATGAAAGGGGCTGAGGACTTTCTACAAGTCATAATGTCAAATTGGAAGCTTGATGATGAAGAGATTGTGAGTGTTGTGTTGGACATATTGCTTGGAGGTTATGAGACCACCGCAGCTCTCATGGGATTGATTGTTTATTTTCTTGCACATTCACCCAATCTTCTTACAAAATTAAGGGTATGTATATTATTTGTTTTGGTGGTTGTTACATTGTGACGATCATTTTTATAGTGTTAAATTATATGTATGGTTATATTGTATCAGCTACATTAAATGACGTCTCGATTAATCATATTGACTATTTCCATTTCAATTATAGTTGTTATTTTTGTGTTTTTGAAAATGATGATGCAGGAAGAACATCAAGCAATAAGCATTGGTAAAAAGATGGAGAAGGTCTCAACTTGGAAGATTACAAGCAAATGGAGTTCACATGCAATGTAA

mRNA sequence

ATGAGACAAAAAAACCAGTGGAAGATCATCACTTGTAAACGCCATGGAAGTTCTGCAAACTTCAGCCCCATCATCATAGCTTTGAGCTTGGCAATGGCGGCAATCATCTGCCGCTCTCTTCTGAAACTCTTTGCAACAGCAGCGCCAAACCCTAATTTGCCTCCCGGCTGCATGGGCTTCCCCATCGTCGGAGAGACTCTCGGCTTCCTCAAGCCTCACGATTCAAACTCCATTGGAACCTTCCTGCACGACCACTGCTCCAGGTTCGGGAAGGTTTTCAGATCCCATCTATTCGGTTCGCCGGCGATAGTGTCGTGCGATCGAGAGCTTAATATGTTCATTCTTCAGAACGACGACAAGCTTTTCAAAGTGAGCTATCCGAAGGCCATGCATGGCATTCTCGGAAGCAATTCGTTGATCATTGCCACCGGAGACACCCACCGGAAGCTGAGAAACGTCGTCGTTAGCTTCATCAGCTGGTGCAAGTCCAGACCTAGTTTCCTTGAGTGCGTGGAGAAGCTGGTTCTCTCTTTAATGGAGTCCTGGAGATGCCAAAACCAAGTGCTTTTCTTCAAAGAAGTGAAGATGTTTGCTCTGAGCGTTGTGGTGAAGGAACTGCTGGGCATCGAACCAGACGAAGCTATAGGGTCAAAGATATTGGAAGAGTTTGAAACGTATATGAAAGGCTTTGTTTCTTTGCCTCTAAATCTCCTGGAACTCCATACTCTAAGGCTGTCAAGAGGGAGGCTTTCTTGCATTGTGAAAGAGATGATGAGAGAGAGGAGGATGAGGGGATTATTGGTGGGGGTGGAAATAATGAAAGGGGCTGAGGACTTTCTACAAGTCATAATGTCAAATTGGAAGCTTGATGATGAAGAGATTGTGAGTGTTGTGTTGGACATATTGCTTGGAGGTTATGAGACCACCGCAGCTCTCATGGGATTGATTGTTTATTTTCTTGCACATTCACCCAATCTTCTTACAAAATTAAGGGAAGAACATCAAGCAATAAGCATTGGTAAAAAGATGGAGAAGGTCTCAACTTGGAAGATTACAAGCAAATGGAGTTCACATGCAATGTAA

Coding sequence (CDS)

ATGAGACAAAAAAACCAGTGGAAGATCATCACTTGTAAACGCCATGGAAGTTCTGCAAACTTCAGCCCCATCATCATAGCTTTGAGCTTGGCAATGGCGGCAATCATCTGCCGCTCTCTTCTGAAACTCTTTGCAACAGCAGCGCCAAACCCTAATTTGCCTCCCGGCTGCATGGGCTTCCCCATCGTCGGAGAGACTCTCGGCTTCCTCAAGCCTCACGATTCAAACTCCATTGGAACCTTCCTGCACGACCACTGCTCCAGGTTCGGGAAGGTTTTCAGATCCCATCTATTCGGTTCGCCGGCGATAGTGTCGTGCGATCGAGAGCTTAATATGTTCATTCTTCAGAACGACGACAAGCTTTTCAAAGTGAGCTATCCGAAGGCCATGCATGGCATTCTCGGAAGCAATTCGTTGATCATTGCCACCGGAGACACCCACCGGAAGCTGAGAAACGTCGTCGTTAGCTTCATCAGCTGGTGCAAGTCCAGACCTAGTTTCCTTGAGTGCGTGGAGAAGCTGGTTCTCTCTTTAATGGAGTCCTGGAGATGCCAAAACCAAGTGCTTTTCTTCAAAGAAGTGAAGATGTTTGCTCTGAGCGTTGTGGTGAAGGAACTGCTGGGCATCGAACCAGACGAAGCTATAGGGTCAAAGATATTGGAAGAGTTTGAAACGTATATGAAAGGCTTTGTTTCTTTGCCTCTAAATCTCCTGGAACTCCATACTCTAAGGCTGTCAAGAGGGAGGCTTTCTTGCATTGTGAAAGAGATGATGAGAGAGAGGAGGATGAGGGGATTATTGGTGGGGGTGGAAATAATGAAAGGGGCTGAGGACTTTCTACAAGTCATAATGTCAAATTGGAAGCTTGATGATGAAGAGATTGTGAGTGTTGTGTTGGACATATTGCTTGGAGGTTATGAGACCACCGCAGCTCTCATGGGATTGATTGTTTATTTTCTTGCACATTCACCCAATCTTCTTACAAAATTAAGGGAAGAACATCAAGCAATAAGCATTGGTAAAAAGATGGAGAAGGTCTCAACTTGGAAGATTACAAGCAAATGGAGTTCACATGCAATGTAA

Protein sequence

MRQKNQWKIITCKRHGSSANFSPIIIALSLAMAAIICRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRLSRGRLSCIVKEMMRERRMRGLLVGVEIMKGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTKLREEHQAISIGKKMEKVSTWKITSKWSSHAM

Homology

[Close]

BLAST of Sgr024142 vs. NCBI nr
Match: KAG6602402.1 (Cytochrome P450 724B1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 486.5 bits (1251), Expect = 2.0e-133
Identity = 255/328 (77.74%), Postives = 286/328 (87.20%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFGKVF+SHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL CV+KL +SLM+SWR Q +VLF KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLL--ELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGIE +E+IGS+IL+EFETYMKGFVSLPLNLL    +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIMK-GAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           EMMRER+ +GL+VGVE  +   E+FL VIMSNWKLD+EEIVSVVLDILLGGYETTA LMG
Sbjct: 245 EMMRERKKKGLVVGVEKERDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS P++L KL+EEH+ I  GK
Sbjct: 305 LIVYFLAHSPPHVLAKLKEEHEGIRNGK 332

[Close]

BLAST of Sgr024142 vs. NCBI nr
Match: XP_022990088.1 (cytochrome P450 724B1 [Cucurbita maxima])

HSP 1 Score: 483.8 bits (1244), Expect = 1.3e-132
Identity = 253/328 (77.13%), Postives = 283/328 (86.28%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLSIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFG VF+SHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL  V+KL +SLMESWRCQ +V F KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNL--LELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGIE +E++G +IL+EFETYMKGFVSLPLNL     +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIM-KGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           EMM+ER+ +GL+VGVE      E+FL VIMSNWKLD+EEIVSVVLDILLGGYETTA LMG
Sbjct: 245 EMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS PN+L KL+EEH+AI  GK
Sbjct: 305 LIVYFLAHSPPNVLAKLKEEHEAIRNGK 332

[Close]

BLAST of Sgr024142 vs. NCBI nr
Match: XP_022922169.1 (cytochrome P450 724B1 [Cucurbita moschata])

HSP 1 Score: 483.4 bits (1243), Expect = 1.7e-132
Identity = 254/328 (77.44%), Postives = 284/328 (86.59%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFGKVF+SHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL CV+KL +SLM+SWR Q +VLF KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLL--ELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGIE +E+IGS+IL+EFETYMKGFVSLPLNLL    +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIM-KGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           EMMRER+ +GL+VGVE      E+FL VIMSNWKLD+EE VSVVLDILLGGYETTA LMG
Sbjct: 245 EMMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEETVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS P++L KL+EEH+ I  GK
Sbjct: 305 LIVYFLAHSPPHVLAKLKEEHEGIRNGK 332

[Close]

BLAST of Sgr024142 vs. NCBI nr
Match: XP_023550580.1 (cytochrome P450 724B1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-132
Identity = 253/328 (77.13%), Postives = 284/328 (86.59%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFGKVF+SHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL CV+KL +SLM+SWR Q +VLF KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNL--LELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGI+ +E+IGS+IL+EFETYMKGFVSLPLNL     +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIM-KGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           E+MRER+ +GL+VGVE      E+FL VIMSNWKLD+EEIVSVVLDILLGGYETTA LMG
Sbjct: 245 EIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS PN+L  L+EEH+AI  GK
Sbjct: 305 LIVYFLAHSPPNVLANLKEEHEAIRNGK 332

[Close]

BLAST of Sgr024142 vs. NCBI nr
Match: XP_023550585.1 (cytochrome P450 724B1 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-132
Identity = 253/328 (77.13%), Postives = 284/328 (86.59%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFGKVF+SHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQRHCSRFGKVFKSHLFGSRAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL CV+KL +SLM+SWR Q +VLF KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNL--LELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGI+ +E+IGS+IL+EFETYMKGFVSLPLNL     +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIDANESIGSRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIM-KGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           E+MRER+ +GL+VGVE      E+FL VIMSNWKLD+EEIVSVVLDILLGGYETTA LMG
Sbjct: 245 EIMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS PN+L  L+EEH+AI  GK
Sbjct: 305 LIVYFLAHSPPNVLANLKEEHEAIRNGK 332

[Close]

BLAST of Sgr024142 vs. ExPASy Swiss-Prot
Match: Q6F4F5 (Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.3e-71
Identity = 139/331 (41.99%), Postives = 216/331 (65.26%), Query Frame = 0


Query: 24  IIIALSLAMAAIICRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNSIGTFLH 83
           +++A  + + A++   +L  F     NP  P G  G+P++GETL FL PH SN++G+FL 
Sbjct: 6   LVLAALVILLALLLTLVLSHFLPLLLNPKAPKGSFGWPLLGETLRFLSPHASNTLGSFLE 65

Query: 84  DHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIAT 143
           DHCSR+G+VF+SHLF +P IVSCD+ELN FILQN+++LF+ SYP+ +HGILG +S+++  
Sbjct: 66  DHCSRYGRVFKSHLFCTPTIVSCDQELNHFILQNEERLFQCSYPRPIHGILGKSSMLVVL 125

Query: 144 GDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQ-------NQVLFFKEVKM 203
           G+ H++LRN+ ++ ++  K +PS+L  +EK+ L ++ SW  +       N + F +E + 
Sbjct: 126 GEDHKRLRNLALALVTSTKLKPSYLGDIEKIALHIVGSWHGKSKDKGMVNVIAFCEEARK 185

Query: 204 FALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRL--SRGRLSCIV 263
           FA SV+VK++LG+ P+E + + ILE+F  +MKG +S PL +      +   +R R+S  V
Sbjct: 186 FAFSVIVKQVLGLSPEEPVTAMILEDFLAFMKGLISFPLYIPGTPYAKAVQARARISSTV 245

Query: 264 KEMMRERRMRGLLVGVEIMKGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 323
           K ++ ERR  G            DFL V++S+ +L DEE VS VLD LLGGYETT+ L+ 
Sbjct: 246 KGIIEERRNAG-------SSNKGDFLDVLLSSNELSDEEKVSFVLDSLLGGYETTSLLIS 305

Query: 324 LIVYFLAHSPNLLTKLREEHQAISIGKKMEK 346
           ++VYFL  S   L  ++ EH+ I   K+ ++
Sbjct: 306 MVVYFLGQSAQDLELVKREHEGIRSKKEKDE 329

[Close]

BLAST of Sgr024142 vs. ExPASy Swiss-Prot
Match: O64989 (Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2)

HSP 1 Score: 205.3 bits (521), Expect = 1.2e-51
Identity = 109/313 (34.82%), Postives = 183/313 (58.47%), Query Frame = 0


Query: 52  NLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELN 111
           NLPPG  G+P +GET+G+LKP+ + ++G F+  H S++GK++RS+LFG P IVS D  LN
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 112 MFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECV 171
            FILQN+ +LF+ SYP+++ GILG  S+++  GD HR +R++ ++F+S  + R   L+ V
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 172 EKLVLSLMESWRCQNQVLFFK-EVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGF 231
           E+  L +++SW+ QN +   + E K F  +++ K ++ ++P E    ++ +E+ T+MKG 
Sbjct: 158 ERHTLFVLDSWQ-QNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGV 217

Query: 232 VSLPLNL--LELHTLRLSRGRLSCIVKEMMRERRM-------------------RGLLVG 291
           VS PLNL     H    SR  +   ++  M ER++                         
Sbjct: 218 VSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDH 277

Query: 292 VEIMKGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTK 343
           V   +  +D L  ++ +  L  E+I+ ++L +L  G+ET++  + L ++FL   P  + +
Sbjct: 278 VRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEE 337

[Close]

BLAST of Sgr024142 vs. ExPASy Swiss-Prot
Match: Q5CCK3 (Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 7.8e-48
Identity = 100/289 (34.60%), Postives = 168/289 (58.13%), Query Frame = 0


Query: 52  NLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELN 111
           NLPPG  G+P+VGET G+L+ H + S+G F+  H +R+GK++RS LFG   +VS D  LN
Sbjct: 53  NLPPGAAGWPLVGETFGYLRAHPATSVGRFMEQHIARYGKIYRSSLFGERTVVSADAGLN 112

Query: 112 MFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECV 171
            +ILQN+ +LF+ SYP+++ GILG  S+++  GD HR++R + ++F+S  + R   L  V
Sbjct: 113 RYILQNEGRLFECSYPRSIGGILGKWSMLVLVGDPHREMRAISLNFLSSVRLRAVLLPEV 172

Query: 172 EKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGFV 231
           E+  L ++ +W   +      + K F  +++ K ++ ++P E    ++  E+ T+MKG V
Sbjct: 173 ERHTLLVLRAWPPSSTFSAQHQAKKFTFNLMAKNIMSMDPGEEETERLRREYITFMKGVV 232

Query: 232 SLPLNL--LELHTLRLSRGRLSCIVKEMMRERRMRGLLVGVEIMKGAEDFLQVIMSNWKL 291
           S PLNL          SR  +  +++  M ER  +  L   +     +D L   +    L
Sbjct: 233 SAPLNLPGTPYWKALKSRAAILGVIERKMEERVEK--LSKEDASVEQDDLLGWALKQSNL 292

Query: 292 DDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTKLREEHQAIS 339
             E+I+ ++L +L  G+ET++  + L ++FL   P  + +LREEH  I+
Sbjct: 293 SKEQILDLLLSLLFAGHETSSMALALAIFFLEGCPKAVQELREEHLGIA 339

[Close]

BLAST of Sgr024142 vs. ExPASy Swiss-Prot
Match: Q42569 (Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 4.6e-40
Identity = 106/334 (31.74%), Postives = 176/334 (52.69%), Query Frame = 0


Query: 27  ALSLAMAAIICRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHC 86
           A  L +++I    LL L  T      LPPG +G P++GET   +  + + +   F+ +  
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 64

Query: 87  SRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDT 146
           +R+G VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  
Sbjct: 65  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 124

Query: 147 HRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKEL 206
           H+++ ++ +SF +    +   +  +++LV   ++SW   ++VL  +E K     + VK+L
Sbjct: 125 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQL 184

Query: 207 LGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRLSRGRLSCIVKEMMRERRMRGL 266
           +  +P E     + +E+   ++GF SLPL L             S   ++ ++ RR    
Sbjct: 185 MSFDPGE-WSESLRKEYLLVIEGFFSLPLPL------------FSTTYRKAIQARRKVAE 244

Query: 267 LVGVEIMK-------GAE---DFLQVIM-SNWKLDDEEIVSVVLDILLGGYETTAALMGL 326
            + V +MK       GAE   D L  ++ ++    DEEIV  ++ +L+ GYETT+ +M L
Sbjct: 245 ALTVVVMKRREEEEEGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTL 304

Query: 327 IVYFLAHSPNLLTKLREEHQAISIGKKMEKVSTW 350
            V FL  +P  L +L+EEH+ I   K       W
Sbjct: 305 AVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEW 323

[Close]

BLAST of Sgr024142 vs. ExPASy Swiss-Prot
Match: Q50EK6 (Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 4.0e-36
Identity = 90/301 (29.90%), Postives = 156/301 (51.83%), Query Frame = 0


Query: 52  NLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHCSRF-GKVFRSHLFGSPAIVSCDREL 111
           +LPPG  G+P++GET  + +   SN    F+ D   R+   VF SHLFGS A++S D + 
Sbjct: 44  HLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDVFVSHLFGSQAVISSDPQF 103

Query: 112 NMFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLEC 171
           N ++LQN+ + F+  YPKA+  ++G   L+   GD  RKL  + V+ + + + +  F+E 
Sbjct: 104 NKYVLQNEGRFFQAHYPKALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEE 163

Query: 172 VEKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGF 231
           ++ LV S ++ W  + ++    E     L+++ K+LL + P +   ++I E F  Y    
Sbjct: 164 IQNLVHSTLDRWVDKKEIALQNECHQMVLNLMAKQLLDLSPSKET-NEICELFVDYTNAV 223

Query: 232 VSLPLNLLELHTLRLSRGRLSCI--VKEMMRERRMRGLLVGVEIMKGAEDFLQVIMSNWK 291
           +++P+ +      +  + R   I  +  M++ERR    +V        +D L  ++    
Sbjct: 224 IAIPIKIPGSTYAKGLKARELLIRKISNMIKERRDHPHIV-------HKDLLTKLLEEDS 283

Query: 292 LDDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTKLREEHQAISIGKKMEKVST 350
           + DE I   +L +L  G+ET++  M   + FL   P  LT+++EEH AI   K   K   
Sbjct: 284 ISDEIICDFILFLLFAGHETSSRAMTFAIKFLTTCPKALTQMKEEHDAILKAKGGHKKLE 336

[Close]

BLAST of Sgr024142 vs. ExPASy TrEMBL
Match: A0A6J1JS89 (cytochrome P450 724B1 OS=Cucurbita maxima OX=3661 GN=LOC111487092 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.2e-133
Identity = 253/328 (77.13%), Postives = 283/328 (86.28%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLSIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFG VF+SHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQYHCSRFGTVFKSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL  V+KL +SLMESWRCQ +V F KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHSVDKLSVSLMESWRCQTRVFFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNL--LELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGIE +E++G +IL+EFETYMKGFVSLPLNL     +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIEANESVGLRILDEFETYMKGFVSLPLNLPGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIM-KGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           EMM+ER+ +GL+VGVE      E+FL VIMSNWKLD+EEIVSVVLDILLGGYETTA LMG
Sbjct: 245 EMMKERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEEIVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS PN+L KL+EEH+AI  GK
Sbjct: 305 LIVYFLAHSPPNVLAKLKEEHEAIRNGK 332

[Close]

BLAST of Sgr024142 vs. ExPASy TrEMBL
Match: A0A6J1E2M5 (cytochrome P450 724B1 OS=Cucurbita moschata OX=3662 GN=LOC111430228 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 8.2e-133
Identity = 254/328 (77.44%), Postives = 284/328 (86.59%), Query Frame = 0


Query: 19  ANFSPIIIALSLAMAAII-CRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNS 78
           A+FSP+ IALSLAMAA+I C+ LLKLFA A P+P+LPPGCMGFP VGETL FLKPH SNS
Sbjct: 5   ASFSPLTIALSLAMAAVILCQLLLKLFAAATPSPSLPPGCMGFPFVGETLSFLKPHHSNS 64

Query: 79  IGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 138
           IGTFL  HCSRFGKVF+SHLFGS AIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN
Sbjct: 65  IGTFLQRHCSRFGKVFKSHLFGSWAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSN 124

Query: 139 SLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMF 198
           SLIIA GDTHRKLRNVVVSF+SWCKS P+FL CV+KL +SLM+SWR Q +VLF KEVKMF
Sbjct: 125 SLIIAAGDTHRKLRNVVVSFVSWCKSNPTFLHCVDKLSVSLMDSWRSQTRVLFCKEVKMF 184

Query: 199 ALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLL--ELHTLRLSRGRLSCIVK 258
           ALSV+VKELLGIE +E+IGS+IL+EFETYMKGFVSLPLNLL    +    +R +LS IVK
Sbjct: 185 ALSVMVKELLGIEANESIGSRILDEFETYMKGFVSLPLNLLGTPYYKAVKARAKLSGIVK 244

Query: 259 EMMRERRMRGLLVGVEIM-KGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMG 318
           EMMRER+ +GL+VGVE      E+FL VIMSNWKLD+EE VSVVLDILLGGYETTA LMG
Sbjct: 245 EMMRERKKKGLVVGVEKEGDDHENFLDVIMSNWKLDEEETVSVVLDILLGGYETTATLMG 304

Query: 319 LIVYFLAHS-PNLLTKLREEHQAISIGK 342
           LIVYFLAHS P++L KL+EEH+ I  GK
Sbjct: 305 LIVYFLAHSPPHVLAKLKEEHEGIRNGK 332

[Close]

BLAST of Sgr024142 vs. ExPASy TrEMBL
Match: A0A6J1BWX9 (cytochrome P450 724B1 OS=Momordica charantia OX=3673 GN=LOC111006083 PE=3 SV=1)

HSP 1 Score: 476.1 bits (1224), Expect = 1.3e-130
Identity = 254/334 (76.05%), Postives = 281/334 (84.13%), Query Frame = 0


Query: 17  SSANFSP---IIIALSLAMAAIICRSLLKL-FATAAPN-PNLPPGCMGFPIVGETLGFLK 76
           ++A+ SP   ++IALSLAMAAII  SLLKL    AA N PNLPPG MGFP +G+TLG LK
Sbjct: 6   AAASLSPATILVIALSLAMAAIISHSLLKLSVIKAAQNYPNLPPGSMGFPFLGQTLGLLK 65

Query: 77  PHDSNSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMH 136
           PH SNSIGTFLH HCSR+GKVF+SHLFG PAIVSCD+ELNMFILQNDDKLFKVSYPKAMH
Sbjct: 66  PHPSNSIGTFLHHHCSRYGKVFKSHLFGWPAIVSCDQELNMFILQNDDKLFKVSYPKAMH 125

Query: 137 GILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFF 196
           GILG+NSLIIA GDTHRKLRNVVVSFI WCKSRPSFL CV  L +SLM+SWR QNQV F 
Sbjct: 126 GILGTNSLIIAAGDTHRKLRNVVVSFIGWCKSRPSFLHCVHNLTISLMDSWRSQNQVFFC 185

Query: 197 KEVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNL--LELHTLRLSRGR 256
           KEVKMFALS++VKELLGIE  EAIGSKILEEFE YMKGFVSLP+NL          +R R
Sbjct: 186 KEVKMFALSIMVKELLGIEAKEAIGSKILEEFEKYMKGFVSLPINLPGTAYSNAVKARER 245

Query: 257 LSCIVKEMMRERRMRGLLVGVEIMKGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETT 316
           LSC+VKEMMRER+ RG++VG +     EDFLQVIMSNWKL+DEEIVSVVLDILLGGYETT
Sbjct: 246 LSCMVKEMMRERKRRGMVVGAD-----EDFLQVIMSNWKLNDEEIVSVVLDILLGGYETT 305

Query: 317 AALMGLIVYFLAHS-PNLLTKLREEHQAISIGKK 343
           A LMGLIVYFLAHS PN+L KL+EEH+AI I K+
Sbjct: 306 ATLMGLIVYFLAHSPPNVLAKLKEEHEAIRIRKR 334

[Close]

BLAST of Sgr024142 vs. ExPASy TrEMBL
Match: A0A0A0KNY1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517880 PE=3 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 1.7e-109
Identity = 222/330 (67.27%), Postives = 258/330 (78.18%), Query Frame = 0


Query: 20  NFSP-IIIALSLAMA-AIICRSLLKLF-ATAAPNPNLPPGCMGFPIVGETLGFLKPHDSN 79
           N SP III L+L+ A +II   LLKLF  T+  NPNLPPG MG P VGETL FL PH SN
Sbjct: 7   NLSPMIIIVLALSFAISIIFHLLLKLFLVTSKKNPNLPPGSMGLPFVGETLSFLNPHHSN 66

Query: 80  SIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGS 139
           SIGTFL  H SR+GK+F+S LFG PAIVSCDRELN F+LQNDDKLFKVSYPKAMH ILG+
Sbjct: 67  SIGTFLQHHFSRYGKIFKSRLFGRPAIVSCDRELNYFVLQNDDKLFKVSYPKAMHNILGT 126

Query: 140 NSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKM 199
           NSL+I+ GDTHRKLR+V+VSFI+ CK+RP+FL  +  L +SL +SWR Q  V F KE+KM
Sbjct: 127 NSLLISAGDTHRKLRSVIVSFITRCKTRPNFLHSLHNLSVSLTDSWRSQTHVSFIKEMKM 186

Query: 200 FALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRL--SRGRLSCIV 259
           FALS++VKE+ GIE  E IG+KI EEFET+M GFVSLPLN       +   +RGRLS IV
Sbjct: 187 FALSLMVKEVFGIEAKELIGTKIFEEFETFMIGFVSLPLNFPGTPYFKAVKARGRLSTIV 246

Query: 260 KEMMRERRMRGLLVGVEIMKGAEDFLQVIMS-NWKLDDEEIVSVVLDILLGGYETTAALM 319
           KEM+RERR RGL+ G       +DFLQV+MS NWKL DEEIVSVVLDI+LG YETTA L+
Sbjct: 247 KEMIRERRKRGLVGG---NNNDDDFLQVLMSNNWKLSDEEIVSVVLDIMLGSYETTATLL 306

Query: 320 GLIVYFLAHS-PNLLTKLREEHQAISIGKK 343
           GLIVYFLAHS PN+L KL+EEHQAI  GK+
Sbjct: 307 GLIVYFLAHSPPNILAKLKEEHQAIRNGKR 333

[Close]

BLAST of Sgr024142 vs. ExPASy TrEMBL
Match: A0A1S3C8C0 (cytochrome P450 724B1 OS=Cucumis melo OX=3656 GN=LOC103498124 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 2.2e-109
Identity = 223/330 (67.58%), Postives = 254/330 (76.97%), Query Frame = 0


Query: 20  NFSPIIIALSLAMA---AIICRSLLK-LFATAAPNPNLPPGCMGFPIVGETLGFLKPHDS 79
           N SP+II L LA++   +II   LLK LF  +  NPNLPPG MG P VGETL FL PH S
Sbjct: 7   NLSPMIIILVLALSFAISIIFHLLLKFLFVISKQNPNLPPGSMGLPFVGETLSFLHPHHS 66

Query: 80  NSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILG 139
           NSIG+FL  H SR+GK+F+SHLFG PAIVSCDRELN F+LQNDDK FKVSYPKAMH ILG
Sbjct: 67  NSIGSFLQHHFSRYGKIFKSHLFGRPAIVSCDRELNNFVLQNDDKFFKVSYPKAMHDILG 126

Query: 140 SNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVK 199
            NSLIIA GDTHRKLR+V+VSFIS CK+RP FL  +  L +SL++SWR + QV F KE+K
Sbjct: 127 KNSLIIAAGDTHRKLRSVIVSFISGCKTRPIFLHSLHNLSVSLIDSWRSRTQVSFVKEMK 186

Query: 200 MFALSVVVKELLGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRL--SRGRLSCI 259
           MFALS+++KE+ GI+  E+IGSKI EEFETYM GFVSLPLNL      +   +RGRLS I
Sbjct: 187 MFALSLMLKEVFGIDAKESIGSKIFEEFETYMIGFVSLPLNLPGTPYFKAVKARGRLSTI 246

Query: 260 VKEMMRERRMRGLLVGVEIMKGAEDFLQVIMS-NWKLDDEEIVSVVLDILLGGYETTAAL 319
           VKEMMRERR R LL G       EDFLQV+MS NWKL DEEIVSV LDILLG YETTA L
Sbjct: 247 VKEMMRERRKRELLGG---NNNNEDFLQVLMSNNWKLSDEEIVSVALDILLGSYETTATL 306

Query: 320 MGLIVYFLAHS-PNLLTKLREEHQAISIGK 342
           +GLIVYFL HS P +L KL+EEHQAI  GK
Sbjct: 307 LGLIVYFLVHSPPKILAKLKEEHQAIRNGK 333

[Close]

BLAST of Sgr024142 vs. TAIR 10
Match: AT5G14400.1 (cytochrome P450, family 724, subfamily A, polypeptide 1 )

HSP 1 Score: 214.9 bits (546), Expect = 1.0e-55
Identity = 121/241 (50.21%), Postives = 164/241 (68.05%), Query Frame = 0


Query: 112 MFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECV 171
           MFILQN+ KLF   YPKAMH ILG  SL++ATG+ HRKL+NV++SFI+  KS+P FL C 
Sbjct: 1   MFILQNEGKLFTSDYPKAMHDILGKYSLLLATGEIHRKLKNVIISFINLTKSKPDFLHCA 60

Query: 172 EKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGFV 231
           E L +S+++SW+   +V F KEVK+F LSV+V +LL I+P++     +L++F +YMKGF+
Sbjct: 61  ENLSISILKSWKNCREVEFHKEVKIFTLSVMVNQLLSIKPEDPARLYVLQDFLSYMKGFI 120

Query: 232 SLPLNL--LELHTLRLSRGRLSCIVKEMMRERRMRGLLVGVEIMKGAEDFLQVIMSNWKL 291
           SLP+ L          +R RLS  V  M++ER      +   I +  EDFL  I+SN  L
Sbjct: 121 SLPIPLPGTGYTNAIKARKRLSARVMGMIKEREREEEDMNNAIRE--EDFLDSIISNEDL 180

Query: 292 DDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTKLREEHQAISIGKKMEKVSTW 351
           + EE VS+VLDILLGG+ET+A  + L+VYFLA SPNLL KL+EEH AI   K   ++  W
Sbjct: 181 NYEEKVSIVLDILLGGFETSATTLSLVVYFLAKSPNLLHKLKEEHAAIRAKKGDGELLNW 239

[Close]

BLAST of Sgr024142 vs. TAIR 10
Match: AT3G50660.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 205.3 bits (521), Expect = 8.3e-53
Identity = 109/313 (34.82%), Postives = 183/313 (58.47%), Query Frame = 0


Query: 52  NLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDRELN 111
           NLPPG  G+P +GET+G+LKP+ + ++G F+  H S++GK++RS+LFG P IVS D  LN
Sbjct: 38  NLPPGKSGWPFLGETIGYLKPYTATTLGDFMQQHVSKYGKIYRSNLFGEPTIVSADAGLN 97

Query: 112 MFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLECV 171
            FILQN+ +LF+ SYP+++ GILG  S+++  GD HR +R++ ++F+S  + R   L+ V
Sbjct: 98  RFILQNEGRLFECSYPRSIGGILGKWSMLVLVGDMHRDMRSISLNFLSHARLRTILLKDV 157

Query: 172 EKLVLSLMESWRCQNQVLFFK-EVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGF 231
           E+  L +++SW+ QN +   + E K F  +++ K ++ ++P E    ++ +E+ T+MKG 
Sbjct: 158 ERHTLFVLDSWQ-QNSIFSAQDEAKKFTFNLMAKHIMSMDPGEEETEQLKKEYVTFMKGV 217

Query: 232 VSLPLNL--LELHTLRLSRGRLSCIVKEMMRERRM-------------------RGLLVG 291
           VS PLNL     H    SR  +   ++  M ER++                         
Sbjct: 218 VSAPLNLPGTAYHKALQSRATILKFIERKMEERKLDIKEEDQEEEEVKTEDEAEMSKSDH 277

Query: 292 VEIMKGAEDFLQVIMSNWKLDDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTK 343
           V   +  +D L  ++ +  L  E+I+ ++L +L  G+ET++  + L ++FL   P  + +
Sbjct: 278 VRKQRTDDDLLGWVLKHSNLSTEQILDLILSLLFAGHETSSVAIALAIFFLQACPKAVEE 337

[Close]

BLAST of Sgr024142 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.3e-41
Identity = 106/334 (31.74%), Postives = 176/334 (52.69%), Query Frame = 0


Query: 27  ALSLAMAAIICRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHC 86
           A  L +++I    LL L  T      LPPG +G P++GET   +  + + +   F+ +  
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 64

Query: 87  SRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDT 146
           +R+G VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  
Sbjct: 65  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 124

Query: 147 HRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKEL 206
           H+++ ++ +SF +    +   +  +++LV   ++SW   ++VL  +E K     + VK+L
Sbjct: 125 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQL 184

Query: 207 LGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRLSRGRLSCIVKEMMRERRMRGL 266
           +  +P E     + +E+   ++GF SLPL L             S   ++ ++ RR    
Sbjct: 185 MSFDPGE-WSESLRKEYLLVIEGFFSLPLPL------------FSTTYRKAIQARRKVAE 244

Query: 267 LVGVEIMK-------GAE---DFLQVIM-SNWKLDDEEIVSVVLDILLGGYETTAALMGL 326
            + V +MK       GAE   D L  ++ ++    DEEIV  ++ +L+ GYETT+ +M L
Sbjct: 245 ALTVVVMKRREEEEEGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTL 304

Query: 327 IVYFLAHSPNLLTKLREEHQAISIGKKMEKVSTW 350
            V FL  +P  L +L+EEH+ I   K       W
Sbjct: 305 AVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEW 323

[Close]

BLAST of Sgr024142 vs. TAIR 10
Match: AT5G05690.2 (Cytochrome P450 superfamily protein )

HSP 1 Score: 166.8 bits (421), Expect = 3.3e-41
Identity = 106/334 (31.74%), Postives = 176/334 (52.69%), Query Frame = 0


Query: 27  ALSLAMAAIICRSLLKLFATAAPNPNLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHC 86
           A  L +++I    LL L  T      LPPG +G P++GET   +  + + +   F+ +  
Sbjct: 5   AFLLLLSSIAAGFLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERV 64

Query: 87  SRFGKVFRSHLFGSPAIVSCDRELNMFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDT 146
           +R+G VF +HLFG P I S D E N F+LQN+ KLF+ SYP ++  +LG +SL++  G  
Sbjct: 65  ARYGSVFMTHLFGEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSL 124

Query: 147 HRKLRNVVVSFISWCKSRPSFLECVEKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKEL 206
           H+++ ++ +SF +    +   +  +++LV   ++SW   ++VL  +E K     + VK+L
Sbjct: 125 HKRMHSLTMSFANSSIIKDHLMLDIDRLVRFNLDSW--SSRVLLMEEAKKITFELTVKQL 184

Query: 207 LGIEPDEAIGSKILEEFETYMKGFVSLPLNLLELHTLRLSRGRLSCIVKEMMRERRMRGL 266
           +  +P E     + +E+   ++GF SLPL L             S   ++ ++ RR    
Sbjct: 185 MSFDPGE-WSESLRKEYLLVIEGFFSLPLPL------------FSTTYRKAIQARRKVAE 244

Query: 267 LVGVEIMK-------GAE---DFLQVIM-SNWKLDDEEIVSVVLDILLGGYETTAALMGL 326
            + V +MK       GAE   D L  ++ ++    DEEIV  ++ +L+ GYETT+ +M L
Sbjct: 245 ALTVVVMKRREEEEEGAERKKDMLAALLAADDGFSDEEIVDFLVALLVAGYETTSTIMTL 304

Query: 327 IVYFLAHSPNLLTKLREEHQAISIGKKMEKVSTW 350
            V FL  +P  L +L+EEH+ I   K       W
Sbjct: 305 AVKFLTETPLALAQLKEEHEKIRAMKSDSYSLEW 323

[Close]

BLAST of Sgr024142 vs. TAIR 10
Match: AT3G13730.1 (cytochrome P450, family 90, subfamily D, polypeptide 1 )

HSP 1 Score: 135.2 bits (339), Expect = 1.0e-31
Identity = 84/296 (28.38%), Postives = 154/296 (52.03%), Query Frame = 0


Query: 51  PNLPPGCMGFPIVGETLGFLKPHDSNSIGTFLHDHCSRFGKVFRSHLFGSPAIVSCDREL 110
           P  P G +G+P++GET+ F+    S+   +F+      +G+VF+SH+FG+  IVS D E+
Sbjct: 49  PKFPHGSLGWPVIGETIEFVSSAYSDRPESFMDKRRLMYGRVFKSHIFGTATIVSTDAEV 108

Query: 111 NMFILQNDDKLFKVSYPKAMHGILGSNSLIIATGDTHRKLRNVVVSFISWCKSRPSFLEC 170
           N  +LQ+D   F   YPK +  ++G +S+++  G  HR+   +V SF+     +   +  
Sbjct: 109 NRAVLQSDSTAFVPFYPKTVRELMGKSSILLINGSLHRRFHGLVGSFLKSPLLKAQIVRD 168

Query: 171 VEKLVLSLMESWRCQNQVLFFKEVKMFALSVVVKELLGIEPDEAIGSKILEEFETYMKGF 230
           + K +   M+ W     VL     K  A  V+ K L+ +E  E +  ++  EFE ++ G 
Sbjct: 169 MHKFLSESMDLWSEDQPVLLQDVSKTVAFKVLAKALISVEKGEDL-EELKREFENFISGL 228

Query: 231 VSLPLNLLELHTLRLSRGRLSCIVK-EMMRERRMRGLLVGVEIMKGAEDFLQVIM--SNW 290
           +SLP+N       R  + + + + + E + E ++R      E    A+D + V++  S+ 
Sbjct: 229 MSLPINFPGTQLHRSLQAKKNMVKQVERIIEGKIRKTKNKEEDDVIAKDVVDVLLKDSSE 288

Query: 291 KLDDEEIVSVVLDILLGGYETTAALMGLIVYFLAHSPNLLTKLREEHQAISIGKKM 344
            L    I + ++D+++ G+++   L+ L V FL+ SP  L  L EE+  +   K++
Sbjct: 289 HLTHNLIANNMIDMMIPGHDSVPVLITLAVKFLSDSPAALNLLTEENMKLKSLKEL 343

The following BLAST results are available for this feature:

BLAST of Sgr024142 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6602402.1	2.0e-133	77.74	Cytochrome P450 724B1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022990088.1	1.3e-132	77.13	cytochrome P450 724B1 [Cucurbita maxima]	[more]
XP_022922169.1	1.7e-132	77.44	cytochrome P450 724B1 [Cucurbita moschata]	[more]
XP_023550580.1	2.2e-132	77.13	cytochrome P450 724B1 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_023550585.1	2.2e-132	77.13	cytochrome P450 724B1 isoform X2 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Sgr024142 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6F4F5	1.3e-71	41.99	Cytochrome P450 724B1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP724B1 PE=1 ...	[more]
O64989	1.2e-51	34.82	Cytochrome P450 90B1 OS=Arabidopsis thaliana OX=3702 GN=CYP90B1 PE=1 SV=2	[more]
Q5CCK3	7.8e-48	34.60	Cytochrome P450 90B2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP90B2 PE=1 SV...	[more]
Q42569	4.6e-40	31.74	Cytochrome P450 90A1 OS=Arabidopsis thaliana OX=3702 GN=CYP90A1 PE=1 SV=1	[more]
Q50EK6	4.0e-36	29.90	Abietadienol/abietadienal oxidase OS=Pinus taeda OX=3352 GN=CYP720B1 PE=1 SV=1	[more]

BLAST of Sgr024142 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JS89	6.2e-133	77.13	cytochrome P450 724B1 OS=Cucurbita maxima OX=3661 GN=LOC111487092 PE=3 SV=1	[more]
A0A6J1E2M5	8.2e-133	77.44	cytochrome P450 724B1 OS=Cucurbita moschata OX=3662 GN=LOC111430228 PE=3 SV=1	[more]
A0A6J1BWX9	1.3e-130	76.05	cytochrome P450 724B1 OS=Momordica charantia OX=3673 GN=LOC111006083 PE=3 SV=1	[more]
A0A0A0KNY1	1.7e-109	67.27	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G517880 PE=3 SV=1	[more]
A0A1S3C8C0	2.2e-109	67.58	cytochrome P450 724B1 OS=Cucumis melo OX=3656 GN=LOC103498124 PE=3 SV=1	[more]

BLAST of Sgr024142 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14400.1	1.0e-55	50.21	cytochrome P450, family 724, subfamily A, polypeptide 1	[more]
AT3G50660.1	8.3e-53	34.82	Cytochrome P450 superfamily protein	[more]
AT5G05690.1	3.3e-41	31.74	Cytochrome P450 superfamily protein	[more]
AT5G05690.2	3.3e-41	31.74	Cytochrome P450 superfamily protein	[more]
AT3G13730.1	1.0e-31	28.38	cytochrome P450, family 90, subfamily D, polypeptide 1	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 45..353 e-value: 7.8E-53 score: 181.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 50..337
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 54..340 e-value: 3.0E-16 score: 59.2
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 57..349
None	No IPR available	PANTHER	PTHR24286:SF159	CYTOCHROME P450, FAMILY 724, SUBFAMILY A, POLYPEPTIDE 1	coord: 57..349

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr024142.1	Sgr024142.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016132	brassinosteroid biosynthetic process
biological_process	GO:0010268	brassinosteroid homeostasis
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016020	membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen