Sgr023964 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023964 vs. NCBI nr
Match: XP_022134434.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Momordica charantia])

HSP 1 Score: 1266.9 bits (3277), Expect = 0.0e+00
Identity = 634/726 (87.33%), Postives = 669/726 (92.15%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGNSSSKRYKLPSPSPSLSPPPLPAMSSPSQSFRQSP-PAA 60
           MLKKLRRNF+RFKTLINKSF +     YK P  SP LSPPP PAMSSP +  RQSP PAA
Sbjct: 1   MLKKLRRNFKRFKTLINKSFSS-----YKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAA 60

Query: 61  ALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSSV 120
            +PFPQTQS VLPDPS+FFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS+LSS+
Sbjct: 61  PVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSI 120

Query: 121 SVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSANLRFF 180
           ++SYPS+  NSA G+Q+FNADLTVSAAG SGS SQKSHIIS FSDLSVTLDIPSANLR F
Sbjct: 121 AISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSF 180

Query: 181 LVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSSSPIHLAH 240
           LVRGSPFLTFEVFN+T ISISTIHAILSFSSNSSFTKFT+TLNNNQTWLIYSSSPI+  H
Sbjct: 181 LVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTH 240

Query: 241 GLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYKWEKKGWGD 300
            LS+ITSG FSGIIRIAA+PDP CE+ILDRFSSCYPVSG A F NPFCLEYKWEK+GWGD
Sbjct: 241 DLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGD 300

Query: 301 LLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVTWHSINGV 360
           LLMLAHPLHLRLLSG      VLDD KYKSIDG LVG+VGSSWVL  +PVSVTWHSINGV
Sbjct: 301 LLMLAHPLHLRLLSG---GGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGV 360

Query: 361 EEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQVIPELRK 420
             EFHSEIISALVKDVEGLNSAPITTTSSYFYGK+IARAARLALIAEEVNFLQVIPE+RK
Sbjct: 361 GAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRK 420

Query: 421 FLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHIGYFLYAI 480
           FLKGAIEPWLRGTFNGNGFLYDGKWGG VT+QGSSDSGADFGFG+YNDHH+HIGYFLYAI
Sbjct: 421 FLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAI 480

Query: 481 AVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASGLTEFADG 540
           AVLVKIDPAWGRKF+ QAYSLMADFMNLSRR NSSFPRLRCFDLYKLHSWASGLTEFADG
Sbjct: 481 AVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADG 540

Query: 541 RNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDNLYEVDF 600
           RNQES+SEAVNAYYSAALLGLAYGD HLASIGSTL A+EIKAAQTWWQIREGDNLYEVDF
Sbjct: 541 RNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDF 600

Query: 601 ARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKELVDWTL 660
            RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSD GFA+ELV+WTL
Sbjct: 601 ERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTL 660

Query: 661 PSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHSRGGGEEG 720
           PSLGREGVGEGWKGFAYALQG++DKDGAL KIRNL GFDDGNSLSNLLWWIHSRGGG EG
Sbjct: 661 PSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGG-EG 716

Query: 721 DGFGGW 726
            G GGW
Sbjct: 721 SG-GGW 716

BLAST of Sgr023964 vs. NCBI nr
Match: XP_038890545.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Benincasa hispida])

HSP 1 Score: 1251.1 bits (3236), Expect = 0.0e+00
Identity = 622/732 (84.97%), Postives = 662/732 (90.44%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGNSSS------KRYKLPSPSPSLSPPPLPAMSSPSQSFRQ 60
           MLKKL+RNF++FKTL NK F  SSS      KRYK PS SP LSP P P MSSP+Q F Q
Sbjct: 1   MLKKLKRNFKKFKTLTNKRFNYSSSSSSSSFKRYKFPSQSPPLSPRPPPEMSSPTQPFWQ 60

Query: 61  SPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120
           SPP  A  FPQ QS VLPDPS FF+PHLLSSPLPTNSFFQNFTVKNGDQPEY HPYLIKS
Sbjct: 61  SPPPVAALFPQIQSTVLPDPSKFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYFHPYLIKS 120

Query: 121 SLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSA 180
           SLS+VS+SYPSMF NSAFGYQ+FNAD TVS      SVSQK HIIS+FSDLS+TLDIPS 
Sbjct: 121 SLSTVSISYPSMFLNSAFGYQVFNADWTVSEN--PSSVSQKPHIISSFSDLSLTLDIPSG 180

Query: 181 NLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSSSP 240
           NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+ LNNNQTWLIYSSSP
Sbjct: 181 NLRFFLVRGSPFMTFEVFNNTPISISTIHAILSFSSNSSLTKFTVALNNNQTWLIYSSSP 240

Query: 241 IHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYKWEK 300
           I+L H LSQITSG FSGI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYKWEK
Sbjct: 241 INLTHSLSQITSGGFSGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKWEK 300

Query: 301 KGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVTWH 360
            GWG+LLMLAHPLHLRLLSG D  AVVLDDFKYKSIDG LVGVVG SWVL P+PVSVTWH
Sbjct: 301 NGWGNLLMLAHPLHLRLLSGYDSGAVVLDDFKYKSIDGDLVGVVGGSWVLKPDPVSVTWH 360

Query: 361 SINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQVI 420
           SINGV EEF SEII+ALVKDVEGL S+PITTTSSYFYGK+IARAARLALIAEEVNFLQVI
Sbjct: 361 SINGVGEEFQSEIINALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNFLQVI 420

Query: 421 PELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHIGY 480
           PE+RKFLKGAIEPWLRGTFNGNGFLYDGKWGG++T+QGS+DSGADFGFG+YNDHHYHIGY
Sbjct: 421 PEVRKFLKGAIEPWLRGTFNGNGFLYDGKWGGLITQQGSNDSGADFGFGVYNDHHYHIGY 480

Query: 481 FLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASGLT 540
           FLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASGLT
Sbjct: 481 FLYAIAVLVKIDPAWGRKFKTHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASGLT 540

Query: 541 EFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDNL 600
           EFADGRNQES+SEAVNAYYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EGDNL
Sbjct: 541 EFADGRNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGDNL 600

Query: 601 YEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKEL 660
           Y+VDFARENKVVGVLWSNKRDSGLWFAP DWRECRLGIQVLP+LPITE+LFSD GF KEL
Sbjct: 601 YDVDFARENKVVGVLWSNKRDSGLWFAPADWRECRLGIQVLPLLPITEILFSDPGFVKEL 660

Query: 661 VDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHSR- 720
           VDWTLPSLGREGVGEGWKGFAYALQG++DKDGA+E+I+NL GFDDGNSLSNLLWWIHSR 
Sbjct: 661 VDWTLPSLGREGVGEGWKGFAYALQGVYDKDGAVERIQNLTGFDDGNSLSNLLWWIHSRG 720

Query: 721 -GGGEEGDGFGG 725
            GGG+EG+G GG
Sbjct: 721 GGGGDEGEGHGG 730

BLAST of Sgr023964 vs. NCBI nr
Match: XP_008459427.1 (PREDICTED: probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucumis melo] >ADN34284.1 beta-glucan-binding protein [Cucumis melo subsp. melo] >KAA0039392.1 putative endo-1,3(4)-beta-glucanase [Cucumis melo var. makuwa] >TYK00580.1 putative endo-1,3(4)-beta-glucanase [Cucumis melo var. makuwa])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 625/733 (85.27%), Postives = 664/733 (90.59%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSF------GNSSSKRYKL---PSPSPSLSPPPLPAMSSPSQS 60
           MLKKL+ NF++FKTLINK+F       +SSSKRYK    PSP P LSPPP P MSSPSQ 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSP-PPLSPPP-PEMSSPSQP 65

Query: 61  FRQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
           F QSPP  A  FPQTQS VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  FWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSSLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDI 180
           IKSSLS+VS+SYPSMF NSAFG+Q+FNAD TVS      SVSQK HIIS+FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSEN--PSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYS 240
           PS NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPIHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYK 300
           SSPI+L H LSQITSG F GI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKKGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSV 360
           WEKKGWG+LLMLAHPLHLRLL+GD   AVVLD+FKYKSIDG LVGVVG SWVL P+PV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFL 420
           TWHSINGV EEFHSEIISALVKDVEGL S+PITTT+SYFYGK+IARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYH 480
           QVIPE+RKFLKGAIEPWL GTFNGNGFLYDGKWGG+VT+QGS DSG DFGFG+YNDHHYH
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREG 600
           GLTEFADGRNQES+SEAVN YYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFA 660
           DNLYE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSDA F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 KELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIH 720
           KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALEKIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGGEEGDGFGG 725
           SRGGG+E +G GG
Sbjct: 726 SRGGGDEVEGCGG 734

BLAST of Sgr023964 vs. NCBI nr
Match: XP_004141490.1 (probable endo-1,3(4)-beta-glucanase ARB_01444 [Cucumis sativus] >KAE8648959.1 hypothetical protein Csa_007756 [Cucumis sativus])

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 624/732 (85.25%), Postives = 663/732 (90.57%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGN-----SSSKRYKL---PSPSPSLSPPPLPAMSSPSQSF 60
           MLKKL+ NF++ +TLINKSF N     SSSKRYK    PSP P LSPPPL  MSSPSQ F
Sbjct: 6   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSP-PPLSPPPL-EMSSPSQPF 65

Query: 61  RQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 120
            QSPP  A  FPQT+S+VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI
Sbjct: 66  WQSPPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 125

Query: 121 KSSLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIP 180
           KSSLS+VSVSYPSM  NSAFGYQ+FNAD TVS      SVSQK HIIS+FSDLS+TLDIP
Sbjct: 126 KSSLSTVSVSYPSMLSNSAFGYQVFNADWTVSEN--PPSVSQKPHIISSFSDLSLTLDIP 185

Query: 181 SANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSS 240
           S NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIY+S
Sbjct: 186 SGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYAS 245

Query: 241 SPIHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYKW 300
           SPI+L H LSQITSG F+GI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYKW
Sbjct: 246 SPINLTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKW 305

Query: 301 EKKGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVT 360
           EKKGWG+LLMLAHPLHLRLLSGD  +AVVLDDFKYKSIDG LVGVVG SW L P+PV +T
Sbjct: 306 EKKGWGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLT 365

Query: 361 WHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQ 420
           WHSINGV EEFHSEIISALVKDVEGL S+PITTTSSYFYGK+IARAARLALIAEEVN+LQ
Sbjct: 366 WHSINGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQ 425

Query: 421 VIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHI 480
           VIPE+RKFLKGAIEPWL GTFNGNGFLYDGKWGG+VT+QGS DSG DFGFG+YNDHHYHI
Sbjct: 426 VIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHI 485

Query: 481 GYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASG 540
           GYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASG
Sbjct: 486 GYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASG 545

Query: 541 LTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGD 600
           LTEFADGRNQES+SEAVN YYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EGD
Sbjct: 546 LTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGD 605

Query: 601 NLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAK 660
           NLYE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSDA F K
Sbjct: 606 NLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVK 665

Query: 661 ELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHS 720
           ELVDWTLPSLGREGVGEGWKGFAYALQG++DKDGALEKIRNL GFDDGNSLSNLLWWIHS
Sbjct: 666 ELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS 725

Query: 721 RGGGEEGDGFGG 725
           RGGG+E +  GG
Sbjct: 726 RGGGDEVEACGG 733

BLAST of Sgr023964 vs. NCBI nr
Match: KAG7033239.1 (hypothetical protein SDJN02_07293, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1228.4 bits (3177), Expect = 0.0e+00
Identity = 617/730 (84.52%), Postives = 656/730 (89.86%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGNSSSKRYKLPSPSPSLSPPPLP------AMSSPSQSFRQ 60
           MLKKLRRNF+ FKTLI+K+F NS S      SPS     PPLP       MSSPSQSFRQ
Sbjct: 1   MLKKLRRNFKTFKTLISKTFNNSRSSS---SSPSKPYKFPPLPLPLSPSEMSSPSQSFRQ 60

Query: 61  SPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120
           S P A  PFPQTQS VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS
Sbjct: 61  SLPPAVAPFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS 120

Query: 121 SLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSA 180
           SLS+VS+SYPSMF N+AFGYQ+FNAD TVS      SVSQK HIIS+FSDLS+ LD PS 
Sbjct: 121 SLSAVSISYPSMFSNTAFGYQVFNADWTVSEN--PASVSQKPHIISSFSDLSLILDTPSG 180

Query: 181 NLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSSSP 240
           NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIYSSSP
Sbjct: 181 NLRFFLVRGSPFITFEVFNNTPISISTIHAILSFSSNSSQTKFTVTLNNNQTWLIYSSSP 240

Query: 241 IHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYKWEK 300
           I+L H LSQITSG FSGI+RIA LP+P  E+ILDRF SCYPVSG  +F NP  LEYKWEK
Sbjct: 241 INLTHSLSQITSGGFSGIVRIAVLPNPHLESILDRFCSCYPVSGEVNFRNPSSLEYKWEK 300

Query: 301 KGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVTWH 360
            GWG+LLMLAHPLHLRLLS DD +A+VLDDFKYKSIDG LVGVVG SWVL P+PVS+TWH
Sbjct: 301 NGWGNLLMLAHPLHLRLLSSDD-SAIVLDDFKYKSIDGDLVGVVGGSWVLKPDPVSITWH 360

Query: 361 SINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQVI 420
           SING+ EEF SEIISALVKDVEGL S+PITTTSSYFYGKAIARAARLALIAEEVNFLQVI
Sbjct: 361 SINGIGEEFQSEIISALVKDVEGLKSSPITTTSSYFYGKAIARAARLALIAEEVNFLQVI 420

Query: 421 PELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHIGY 480
           PE+RKFLK AIEPWLRGTFNGNGFLYD KWGG+VT+QG+SDSGADFGFG+YNDHHYHIGY
Sbjct: 421 PEVRKFLKDAIEPWLRGTFNGNGFLYDSKWGGLVTQQGASDSGADFGFGVYNDHHYHIGY 480

Query: 481 FLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASGLT 540
           FLYAIAVLVKIDPAWGRKF+ QAYSLMADFMNLSRRS+S FPRLRCFDLYKLHSWASGLT
Sbjct: 481 FLYAIAVLVKIDPAWGRKFRPQAYSLMADFMNLSRRSSSKFPRLRCFDLYKLHSWASGLT 540

Query: 541 EFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDNL 600
           EFADGRNQESSSEAVNAYYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQIR G+NL
Sbjct: 541 EFADGRNQESSSEAVNAYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIRGGENL 600

Query: 601 YEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKEL 660
           YE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ+LP+LPITE LFSDA FAKEL
Sbjct: 601 YETDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQLLPLLPITEALFSDAAFAKEL 660

Query: 661 VDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHSRG 720
           VDWTLPSL REGVGEGWKGFAYALQGI+DKDGA++ IRNL GFDDGNSLSNLLWWIHSRG
Sbjct: 661 VDWTLPSLRREGVGEGWKGFAYALQGIYDKDGAVQNIRNLKGFDDGNSLSNLLWWIHSRG 720

Query: 721 GGEEGDGFGG 725
           GGEEG+GFGG
Sbjct: 721 GGEEGEGFGG 724

BLAST of Sgr023964 vs. ExPASy Swiss-Prot
Match: D4AZ24 (Probable endo-1,3(4)-beta-glucanase ARB_01444 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_01444 PE=1 SV=1)

HSP 1 Score: 161.0 bits (406), Expect = 5.1e-38
Identity = 199/754 (26.39%), Postives = 319/754 (42.31%), Query Frame = 0

Query: 32  SPSPSL-SPPPLPAMSSPSQSFRQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTN 91
           SP+  + S P  P  +   Q   Q  P A  P P    +    P         + P+ TN
Sbjct: 172 SPTSGIPSQPSAPPATMAGQDIFQ--PIAKDPIPANIKSRDDHPVKANHIENPTGPISTN 231

Query: 92  SFFQNFTVKNGDQPEYIHPYLI-------KSSLSSVSVSY------------------PS 151
            F+ NF + N     + HPY +        +S   +++S+                  P 
Sbjct: 232 KFYANFFLGNQTSTTFTHPYTMIWAKGDKNASSFGMAISHVEPSQRATGEPNNKLPGNPV 291

Query: 152 MFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDI--PSANLRFFLVRG 211
            ++ +  G +     L +SA+ +  S +       AFS  ++       S ++ F LV+G
Sbjct: 292 RYYINPVGIK----SLVLSASELKESTTMSVAKPQAFSAQAILRPTGGSSESITFPLVQG 351

Query: 212 SPFLTFEVFNSTAISISTIHAIL------SFSSNSSFTKFTITLNNNQTWLIY-----SS 271
             F+T  ++N+   +I +  A+L      + S      K+ ITL +++ WL+Y      +
Sbjct: 352 MGFIT-AIYNNLQPAIQS--AVLFRKVEPAGSPQGGIFKYKITLEDDKNWLLYVIPENGA 411

Query: 272 SPIHLAHGLSQITSGE--FSGIIRIAALPD-PRCETILDRFSSCY----PVSGVAHFANP 331
            P     G +++ SG   F G+I++A  P     E I D+ +  Y     +SG       
Sbjct: 412 DPKLKLEG-NKLISGPTGFKGVIQVAKNPSAEEGEGIYDKSAGSYATDIKISGSVGTDGT 471

Query: 332 FCLEYKWEKKGWG-DLLMLAHPLHLRLLSGDDPAAVVLDDFKYK-SIDGALVGVVGSSWV 391
              ++ +EK G G  L+M A P H+   S DD       + K   +  G     VG SW 
Sbjct: 472 GTYKFSFEKAGKGAPLVMYALPHHVE--SFDDATKNTKKNMKLSTTTKGMATACVGDSWT 531

Query: 392 LNPN--PVSV---TWHSINGVEEEFHSEIISALVKDVEGLN-----SAPITTTSSYFYGK 451
           +     P+S+    W   +  +    SE     +K V G              S YF GK
Sbjct: 532 MVEGNLPLSMDFAPWKPGSSSQVTL-SEGAKNAIKAVAGNELSQDMELQTNLNSMYFSGK 591

Query: 452 AIAR-AARLALIAEEVNFLQVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTK-- 511
            + + A  +  + E V            LK + + ++         +YD  W G+V+   
Sbjct: 592 GLNKFAGAIYTVQELVGDKAAASGPLNSLKESFKRFVDNK-QQIPLVYDNVWKGVVSSGT 651

Query: 512 QGSSDSGADFGFGLYNDHHYHIGYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRR 571
               D+G DFG  LYNDHH+H GYF+   A+L K+DPAW    K     L+ D  N S  
Sbjct: 652 YEKGDTGLDFGNTLYNDHHFHYGYFILTAAILGKLDPAWLDANKAYVNMLVRDSGN-SVD 711

Query: 572 SNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASI 631
           ++  FP  R FD Y  HSWA GL E +DG++QES+SE     Y+  + G   GD  + + 
Sbjct: 712 NDEHFPFSRAFDWYHGHSWAKGLFESSDGKDQESTSEDTMYAYAIKMWGKTSGDKSMEAR 771

Query: 632 GSTLVALEIKAAQTWWQIREGDNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRL 691
           G+ ++ +  +    ++ + + DN+ +      NKV G+L+ NK D   +F      E   
Sbjct: 772 GNLMLGILARTLNNYF-LMKNDNVNQPKNFIGNKVTGILFENKIDHTTYFGAN--LEYIQ 831

Query: 692 GIQVLPVLPITELLFSDAGFAKELVDWTL-----PSLGREGVGEGWKGFAYALQGIFDKD 716
           GI +LP+LP +    S A F KE  +W        +   E V  GWKG  YA   I D +
Sbjct: 832 GIHMLPLLPNSAYTRS-AEFVKE--EWEAMFASGAAAPAEKVTGGWKGVLYANLAIIDPE 891

BLAST of Sgr023964 vs. ExPASy Swiss-Prot
Match: Q9UT45 (Primary septum endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng1 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 1.1e-37
Identity = 171/687 (24.89%), Postives = 288/687 (41.92%), Query Frame = 0

Query: 83  LSSPLPTNSFFQNFTVKNGDQPEYIHPYLI----KSSLSSVSVSYPSMFFNSAFGYQIFN 142
           LSSP+ TN FF N  + +     Y  P+        ++  + +++      +    ++ +
Sbjct: 78  LSSPIETNKFFDNNLLGSRTNFMYADPFRYWWQSSDTMGGICIAH------TDDNQRVMD 137

Query: 143 ADLTVSA------------AGISGSVSQKSHIISAFSDLSV--TLDIPSANLRFFLVRGS 202
            D T+ +             G SG  S    I+     +S   T    S++++  L  G 
Sbjct: 138 TDDTIPSYYYEPIGICSLGFGASGITSNTDPIVDEIDQMSARFTFSWDSSSMQLTLTEGM 197

Query: 203 PFLTFEVFNSTAISIST---IHAILSFSSNSSFTKFTITLNNNQTWLIY-SSSPIHLAHG 262
              T    N+     S+   I+  +     S+  K+ +T+++N  WLIY     + L   
Sbjct: 198 AVTTAVYTNAIPQIFSSTLYINDFVEVPGTSAVQKYRVTMSDNHVWLIYIYGDSLTLTES 257

Query: 263 LSQ--ITSGEFSGIIRIAALP--DPRCETILDRFSSCY----PVSGVAHFANPFCLEYKW 322
            SQ  + S  F+G I+IA +P  D   E + D ++  Y     +SG    A  +   Y +
Sbjct: 258 TSQMLVGSNTFNGYIQIAKIPLGDGTAEALYDTYAGVYITGISISGYVEDAVGY---YSF 317

Query: 323 EKKGWGD------LLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNP 382
           +    GD        +L H +    +SG    ++VL       ++ A    +  +  +  
Sbjct: 318 DFTTAGDTSVEPLFFLLPHQVD-TAVSGTKVTSIVLASLVSGDMNAAAGNSITFAEAIPQ 377

Query: 383 NPVSVTWHSINGVEEEFHSEIISALVKDVEGLN-----SAPITTTSSYFYGKAIARAARL 442
           +   + W    G  +  +SE    ++ +V G       SA     S Y+ GK +A+ A L
Sbjct: 378 DIGFLPWSPTGG--QIGYSEEALEIIAEVAGTELGEDFSANSNLNSMYYSGKVLAKYAML 437

Query: 443 A-----LIAEEVNFLQVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQG-SSD 502
                 ++ +E +  Q I +L       ++             YD  W G+V+  G S D
Sbjct: 438 CVTINDILGDETSSEQCIQKLEAAFARFVD-----NQQIYPLTYDNTWKGVVSVAGLSGD 497

Query: 503 SGADFGFGLYNDHHYHIGYFLYAIAVLVKIDPAW--GRKFKRQAYSLMADFMNLSRRSNS 562
           S ADFG   YNDHH+H GYF++  AV+  IDP W      K     L+ D  N S  ++ 
Sbjct: 498 SLADFGNSYYNDHHFHYGYFVFTAAVIGHIDPDWINTGNNKEWVNFLVRDVANPS-SNDP 557

Query: 563 SFPRLRCFDLYKLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGST 622
            FP+ R  D+Y  H WASGL E  DG+++ES+SE  N ++   L G   GD  +    + 
Sbjct: 558 YFPKHRMIDIYHGHGWASGLFESNDGKDEESTSEDYNFFFGMKLWGQVIGDSDMEDRANI 617

Query: 623 LVALEIKAAQTWWQIREGDNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQ 682
           ++ +E  A   +    +G+   +    + N V G+ + NK     +F      E   GI 
Sbjct: 618 ILGIERNALNKYMLYADGN--VQPTSMQPNYVAGITFMNKITHTTYFGTN--IEYIQGIH 677

Query: 683 VLPVLPITELLFSDAGFAKELVDW-TLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIR 716
           +LP+ PI+  +    G +  L +W  L +   + V  GW+   YA   I + + + E   
Sbjct: 678 MLPITPISAFI---RGPSFVLAEWNALLASVIDYVDSGWRSLLYANLAIAEPEESYEYFS 736

BLAST of Sgr023964 vs. ExPASy Swiss-Prot
Match: Q12168 (Endo-1,3(4)-beta-glucanase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ACF2 PE=1 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 8.1e-36
Identity = 168/687 (24.45%), Postives = 291/687 (42.36%), Query Frame = 0

Query: 86  PLPTNSFFQNFTVKNGDQPEYIHPY------------------LIKSSLSSVSVSYPSMF 145
           PL TN F+ N  + +  QP + HPY                  L    +   + + P  +
Sbjct: 110 PLETNKFYTNMLLDDNTQPIWTHPYSIWFSRDPELFGLAANHTLASQRVFDTTTNPPRFY 169

Query: 146 FN-SAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSAN-LRFFLVRGSP 205
           FN +     +F A   VS+  I        H+      + + + + S+  + F LV+G  
Sbjct: 170 FNPTNIKSFVFKAREFVSSNDIKLEFRDMKHM-----SMCLLMSLSSSQFIEFPLVQGMG 229

Query: 206 FLTFEVFNSTAISIST---IHAILSFSSNSSFTKFTITLNNNQTWLIYSSSP-------- 265
           F+T  +++     + +     ++   S N  + K+ I L NN+ W++Y +SP        
Sbjct: 230 FVT-AIYHDLGFELRSAVGFRSLERISVNERYGKYNIQLENNRNWILYLTSPDYSFPQDF 289

Query: 266 -IHLAHGLSQITSGEFSGII-RIAALPDPRCETILDRFSSCYPV----SGVA---HFANP 325
            I L    + I+S + +G+I +++A   P     +D  + CYPV    SG     HF N 
Sbjct: 290 QISLLDSNTIISSHKINGLICQLSADSVPS----IDMAAGCYPVYCDLSGQTVDEHFTN- 349

Query: 326 FCLEYKWEKKGW---GDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSW 385
               + +   G+   G  LM A P H    + +     +       ++ G + G + +S+
Sbjct: 350 --YRFNYTVAGYSQSGTTLMYALPHHKAAFTPEMQEREIASSLD-STVKGLMTGYLTNSF 409

Query: 386 --------VLNPNPVSVTWHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSS-YFYGK 445
                    L   PV+++ +      +E  S+I  A V++V+  +    +   S YF GK
Sbjct: 410 DMQVQVPQELGFEPVALSLNKKADYSQEKLSKIREAAVQEVQLSDPQQESNIDSMYFSGK 469

Query: 446 AIARAARLALIAEEV-NFLQVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQG 505
            +A+ A +  +   + +   +  EL   L  A+E ++          YD  W GI++   
Sbjct: 470 ILAKYAWILYVTHYILHDENLTKELLSKLTIAMERFISNQ-QVLPLNYDVSWKGIIS--- 529

Query: 506 SSDSGADFGFGLYNDHHYHIGYFLYAIAVLVKIDP--------AWGRKFKRQAYSLMADF 565
           S  S  DFG   YNDHH+H  Y +   A++  +D         +W    +     L+ D+
Sbjct: 530 SGSSSQDFGNSYYNDHHFHYSYHVITAAIISLVDSDLSGVTNNSWLENNRDWVECLIRDY 589

Query: 566 MNLSRRSNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGD 625
             +    +  FP+ R FD +  HSWA GL    DG+++ES+SE VN+ Y+  L GL  G+
Sbjct: 590 SGVD-NDDPYFPQFRSFDWFNGHSWAKGLFPSGDGKDEESTSEDVNSCYAIKLWGLVTGN 649

Query: 626 IHLASIGSTLVALEIKAAQTWWQIREGDNLYEVDFARENKVVGVLWSNKRDSGLWFA-PP 685
             L  I +  + +     Q+++     + +   +F   NKV G+L+ NK D   +F   P
Sbjct: 650 SKLTDIANLQLGIMRNVFQSYFLYESNNTVQPKEFI-GNKVSGILFENKIDHATYFGMEP 709

Query: 686 DWRECRLGIQVLPVLPITEL--LFSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGI 705
            +      I ++  +PIT          F KE  +  +  +  + V +GWKG       +
Sbjct: 710 QY------IHMIHAIPITSASSWVRTPNFVKEEWEEKMQPI-IDQVNDGWKGIIMLNMAL 769

BLAST of Sgr023964 vs. ExPASy Swiss-Prot
Match: P53753 (Endo-1,3(4)-beta-glucanase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DSE4 PE=1 SV=1)

HSP 1 Score: 146.4 bits (368), Expect = 1.3e-33
Identity = 189/734 (25.75%), Postives = 309/734 (42.10%), Query Frame = 0

Query: 22   NSSSKRYKLPSP--SPSLSPPPLPAMSSPSQSFRQSPPAAALPFPQTQSAVLPDPSNFFS 81
            +SSS++ K  S   S S S       +S + +      A +   P T     P+P +   
Sbjct: 367  SSSSRQTKTSSSSLSTSTSSTATTTENSSTTTIVNLFNAVSTDEPPTVFDRSPNPMSLAD 426

Query: 82   PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI---KSSLSSVSVSYPSMFFNSAFGYQI 141
                  P+ TN F+ N  V + + P +++PY +    SS    +V + ++   S  GY  
Sbjct: 427  GVSNDGPIQTNKFYTNLIVGSQESPAFVYPYSLWKYTSSSYGFAVQHTTVDQYSYGGYDS 486

Query: 142  F-NADLTVSAAGISGSVSQKSH-------------------IISAFSDLSVTLDIPSANL 201
              NA+  V+  GI+  V   S+                   ++S  +D S  L+IP    
Sbjct: 487  SGNAEYLVNPLGIAHVVFSASNFDSSMTMQVDEMTLSSTRVVLSESNDSSNYLEIP---- 546

Query: 202  RFFLVRGSPFLTFEVFNS------TAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIY 261
               LV+G  F T     S      +++  +TI +  S +      K+ ITL N  TWL Y
Sbjct: 547  ---LVQGMGFATGIYHGSLNAKIGSSVGFNTIVSESSSNLAQGILKYRITLLNGVTWLCY 606

Query: 262  SSSPIHL-AHGLSQITSGEF---------SGIIRIAALP-DPRCETILDRFSSCY----P 321
               P  L +   S   S E+           II++A  P +   E   D+ +  Y     
Sbjct: 607  VIGPDDLTSTDFSLEVSSEYEIKASASVDGLIIQLAVAPSETDYEVFYDQAAGMYVTNFK 666

Query: 322  VSGVAHFANPFCLEYKWEKKG---WGDLLMLAHPLHLRLLSG---DDPAAVVLDDFKYKS 381
            + GV+   +    E+ +  +G    G  ++ A P H    S    D    + L       
Sbjct: 667  LQGVSD-GSTATYEFSYTTQGESASGSTMIFALPHHESSFSDIMQDYYTGIQLASTTKGV 726

Query: 382  IDGALVGVVGSSWVLNPNPVSVTWHSING-------------VEEEFHSEIISALVKDVE 441
            ++G L   +  S  LN     + W S  G             + E  +SE+  ++ + + 
Sbjct: 727  MNGYLTTSLQFSTSLNRQISWLPWSSQLGSNLLEYSKEQLQLLAEVANSELQVSISESIS 786

Query: 442  GLNSAPITTTSSYFYGKAIARAARLAL-IAEEVNFLQVIPELRKFLKGAIEPWLRGTFNG 501
            GLN        +Y+ GK I + + + L ++E +          + +K A +  L+     
Sbjct: 787  GLN--------TYYLGKVIDKYSYILLTVSEIIQDEASTKSTLENIKSAFDILLQNE-QT 846

Query: 502  NGFLYDGKWGGIVTK--QGSSDSGADFGFGLYNDHHYHIGYFLYAIAVL----VKIDPAW 561
               +YD K+ G+V+    GS+ +  DFG   YNDHH+H GY ++A AV+     K++  W
Sbjct: 847  YPLIYDTKFNGLVSSGDWGSTSTQYDFGNTYYNDHHFHYGYIIHAAAVIGYVDSKLNGTW 906

Query: 562  GRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASGLTEFADGRNQESSSEAV 621
                K    SL+ D  N S + +  F + R FD +  HSWA+GL E  +G+N+ESSSE  
Sbjct: 907  AADNKDWVNSLVRDVANPSEK-DEYFAQSRMFDWFNGHSWAAGLYENGNGKNEESSSEDY 966

Query: 622  NAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDNLYEVDFARENKVVGVL 681
            N  Y+  L G   GD  +   G  ++++ +K A   +   + DN  E +    NKV G+L
Sbjct: 967  NFAYAMKLWGATIGDQSMELRGDLMISI-MKDAMNDYFYYQNDNTVEPEEIIGNKVSGIL 1026

Query: 682  WSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKELVDWTLPSLGREGVGE 684
            + N  D   +F      E   GI +LP+ P++  + S+    +E      P +  E +  
Sbjct: 1027 FDNIIDYTTYFGTN--TEYIHGIHMLPITPVSSNIRSETFVEEEWQTKIEPII--ESIES 1077

BLAST of Sgr023964 vs. ExPASy Swiss-Prot
Match: Q09850 (Ascus wall endo-1,3(4)-beta-glucanase OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=eng2 PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 1.1e-32
Identity = 169/681 (24.82%), Postives = 286/681 (42.00%), Query Frame = 0

Query: 64  PQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIK----SSLSSV 123
           P   S   P P    + +L   P+ TN F+ N  +     P + HPY +     SS   +
Sbjct: 14  PVFPSRAHPSPPLGLTSNLF--PIQTNKFYGNLYIGTRHNPSWSHPYSVTWLNGSSYYGL 73

Query: 124 SVSY---------------PSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSD 183
           ++S+               P  ++ +  G           A+G   S+ Q  H  S  + 
Sbjct: 74  AISHIDDSQRVFGPDPESVPCQYYFNPAGLYSIIISAREFASGNLLSLDQSRH-FSIQAT 133

Query: 184 LSVTLDIPSANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFT---KFTITL 243
           LS T    S  +   +V G  F++    N T +  S+I  + S  +  +F+   K+ I L
Sbjct: 134 LSATTS-GSGTIILPIVAGMGFVSGYYTNLTPVFNSSI--LFSSITKINFSRGYKYRIQL 193

Query: 244 NNNQTWLIY-------SSSPIHLAHGLSQITSGEFSGIIRIAALPDPRC-----ETILDR 303
            + + W +Y       S+  + LA      TS +F+G+I+I  +P+        +TI D 
Sbjct: 194 TDGKIWFLYAFPTSSSSTFNLTLASNSQLTTSTKFTGLIQICKVPNESVNNSYPDTIYDA 253

Query: 304 FSSCYPVS-GVAHFANPFCLEY--KWEKKGWGDL--LMLAHPLHLRLLSGDDPAAVVLDD 363
            +  Y  S  ++   +    EY  ++   G+ +L  LM A P H++    D  A      
Sbjct: 254 SAGVYTTSISLSAQVSGTTGEYWFRFATAGYTNLNPLMFALPHHMQSFGSDTQA------ 313

Query: 364 FKYKSIDGALVGVVGSSWVLNPNPVSVTWHSI---------------NGVEEEFHSEIIS 423
             YK+  G     +G  +       + TWH I               NG    +    ++
Sbjct: 314 --YKTGLGLASTTMGIMFAY----ATKTWHLIEKNLPTQVGFLPIPWNGGSNTYSPTALA 373

Query: 424 AL----VKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQVIPE--LRKFLKG 483
           A+      D+           S Y  GK +A  A++ L+A  +     +    L K LK 
Sbjct: 374 AIRAACATDINFDVVNASNLDSMYTSGKIVAMYAQVCLVASRILGDSTLTNTGLTK-LKQ 433

Query: 484 AIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHIGYFLYAIAVLV 543
           A+  +   T      +YD  + GI++  G S   AD+G   YNDHH+H GY +YA AV+ 
Sbjct: 434 AMARFTTNT-QMYPLVYDTTYKGIISTAGYSSPLADYGNTYYNDHHFHWGYHIYACAVIG 493

Query: 544 KIDPAW-GRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASGLTEFADGRNQ 603
            +DP+W      R   +L+ D  N S  S++ F   R FD +  HSWA+G+ E  DG+++
Sbjct: 494 LLDPSWLVNDNIRYVNALLRDSANPS-ESDTYFAMFRNFDWFVGHSWATGIFESGDGKDE 553

Query: 604 ESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDNLYEVDFARE 663
           ES+SE  N  Y+  L G+   D  L +  + ++A+   +  T+  +    ++        
Sbjct: 554 ESTSEDFNFLYATKLWGMVRNDTVLINRANLMLAVLKNSLNTYIYMTPTTSVQPSQIL-G 613

Query: 664 NKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKELVDWTLPSL 684
           N V G+ + NK D   +F+  ++  C+ GI ++P  PI+  L S +   ++      P +
Sbjct: 614 NYVTGITFMNKVDYATYFSAEEY--CKQGIHMIPTTPISGYLRSPSYVQQDWNAKIAPII 668

BLAST of Sgr023964 vs. ExPASy TrEMBL
Match: A0A6J1BYQ9 (Endo-1,3(4)-beta-glucanase OS=Momordica charantia OX=3673 GN=LOC111006684 PE=3 SV=1)

HSP 1 Score: 1266.9 bits (3277), Expect = 0.0e+00
Identity = 634/726 (87.33%), Postives = 669/726 (92.15%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGNSSSKRYKLPSPSPSLSPPPLPAMSSPSQSFRQSP-PAA 60
           MLKKLRRNF+RFKTLINKSF +     YK P  SP LSPPP PAMSSP +  RQSP PAA
Sbjct: 1   MLKKLRRNFKRFKTLINKSFSS-----YKFPFQSPPLSPPPPPAMSSPFEPLRQSPRPAA 60

Query: 61  ALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSSV 120
            +PFPQTQS VLPDPS+FFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKS+LSS+
Sbjct: 61  PVPFPQTQSTVLPDPSDFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSALSSI 120

Query: 121 SVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSANLRFF 180
           ++SYPS+  NSA G+Q+FNADLTVSAAG SGS SQKSHIIS FSDLSVTLDIPSANLR F
Sbjct: 121 AISYPSIVCNSAVGFQVFNADLTVSAAGNSGSFSQKSHIISEFSDLSVTLDIPSANLRSF 180

Query: 181 LVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSSSPIHLAH 240
           LVRGSPFLTFEVFN+T ISISTIHAILSFSSNSSFTKFT+TLNNNQTWLIYSSSPI+  H
Sbjct: 181 LVRGSPFLTFEVFNNTPISISTIHAILSFSSNSSFTKFTVTLNNNQTWLIYSSSPINFTH 240

Query: 241 GLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYKWEKKGWGD 300
            LS+ITSG FSGIIRIAA+PDP CE+ILDRFSSCYPVSG A F NPFCLEYKWEK+GWGD
Sbjct: 241 DLSKITSGGFSGIIRIAAVPDPHCESILDRFSSCYPVSGEAKFGNPFCLEYKWEKRGWGD 300

Query: 301 LLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVTWHSINGV 360
           LLMLAHPLHLRLLSG      VLDD KYKSIDG LVG+VGSSWVL  +PVSVTWHSINGV
Sbjct: 301 LLMLAHPLHLRLLSG---GGAVLDDLKYKSIDGDLVGIVGSSWVLQSDPVSVTWHSINGV 360

Query: 361 EEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQVIPELRK 420
             EFHSEIISALVKDVEGLNSAPITTTSSYFYGK+IARAARLALIAEEVNFLQVIPE+RK
Sbjct: 361 GAEFHSEIISALVKDVEGLNSAPITTTSSYFYGKSIARAARLALIAEEVNFLQVIPEVRK 420

Query: 421 FLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHIGYFLYAI 480
           FLKGAIEPWLRGTFNGNGFLYDGKWGG VT+QGSSDSGADFGFG+YNDHH+HIGYFLYAI
Sbjct: 421 FLKGAIEPWLRGTFNGNGFLYDGKWGGFVTQQGSSDSGADFGFGVYNDHHFHIGYFLYAI 480

Query: 481 AVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASGLTEFADG 540
           AVLVKIDPAWGRKF+ QAYSLMADFMNLSRR NSSFPRLRCFDLYKLHSWASGLTEFADG
Sbjct: 481 AVLVKIDPAWGRKFRPQAYSLMADFMNLSRRPNSSFPRLRCFDLYKLHSWASGLTEFADG 540

Query: 541 RNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDNLYEVDF 600
           RNQES+SEAVNAYYSAALLGLAYGD HLASIGSTL A+EIKAAQTWWQIREGDNLYEVDF
Sbjct: 541 RNQESTSEAVNAYYSAALLGLAYGDTHLASIGSTLTAMEIKAAQTWWQIREGDNLYEVDF 600

Query: 601 ARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKELVDWTL 660
            RENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSD GFA+ELV+WTL
Sbjct: 601 ERENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEVLFSDVGFARELVEWTL 660

Query: 661 PSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHSRGGGEEG 720
           PSLGREGVGEGWKGFAYALQG++DKDGAL KIRNL GFDDGNSLSNLLWWIHSRGGG EG
Sbjct: 661 PSLGREGVGEGWKGFAYALQGVYDKDGALGKIRNLGGFDDGNSLSNLLWWIHSRGGG-EG 716

Query: 721 DGFGGW 726
            G GGW
Sbjct: 721 SG-GGW 716

BLAST of Sgr023964 vs. ExPASy TrEMBL
Match: A0A5A7T7D7 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G001960 PE=3 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 625/733 (85.27%), Postives = 664/733 (90.59%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSF------GNSSSKRYKL---PSPSPSLSPPPLPAMSSPSQS 60
           MLKKL+ NF++FKTLINK+F       +SSSKRYK    PSP P LSPPP P MSSPSQ 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSP-PPLSPPP-PEMSSPSQP 65

Query: 61  FRQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
           F QSPP  A  FPQTQS VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  FWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSSLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDI 180
           IKSSLS+VS+SYPSMF NSAFG+Q+FNAD TVS      SVSQK HIIS+FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSEN--PSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYS 240
           PS NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPIHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYK 300
           SSPI+L H LSQITSG F GI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKKGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSV 360
           WEKKGWG+LLMLAHPLHLRLL+GD   AVVLD+FKYKSIDG LVGVVG SWVL P+PV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFL 420
           TWHSINGV EEFHSEIISALVKDVEGL S+PITTT+SYFYGK+IARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYH 480
           QVIPE+RKFLKGAIEPWL GTFNGNGFLYDGKWGG+VT+QGS DSG DFGFG+YNDHHYH
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREG 600
           GLTEFADGRNQES+SEAVN YYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFA 660
           DNLYE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSDA F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 KELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIH 720
           KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALEKIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGGEEGDGFGG 725
           SRGGG+E +G GG
Sbjct: 726 SRGGGDEVEGCGG 734

BLAST of Sgr023964 vs. ExPASy TrEMBL
Match: E5GCT5 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo subsp. melo OX=412675 PE=3 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 625/733 (85.27%), Postives = 664/733 (90.59%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSF------GNSSSKRYKL---PSPSPSLSPPPLPAMSSPSQS 60
           MLKKL+ NF++FKTLINK+F       +SSSKRYK    PSP P LSPPP P MSSPSQ 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSP-PPLSPPP-PEMSSPSQP 65

Query: 61  FRQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
           F QSPP  A  FPQTQS VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  FWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSSLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDI 180
           IKSSLS+VS+SYPSMF NSAFG+Q+FNAD TVS      SVSQK HIIS+FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSEN--PSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYS 240
           PS NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPIHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYK 300
           SSPI+L H LSQITSG F GI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKKGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSV 360
           WEKKGWG+LLMLAHPLHLRLL+GD   AVVLD+FKYKSIDG LVGVVG SWVL P+PV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFL 420
           TWHSINGV EEFHSEIISALVKDVEGL S+PITTT+SYFYGK+IARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYH 480
           QVIPE+RKFLKGAIEPWL GTFNGNGFLYDGKWGG+VT+QGS DSG DFGFG+YNDHHYH
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREG 600
           GLTEFADGRNQES+SEAVN YYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFA 660
           DNLYE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSDA F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 KELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIH 720
           KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALEKIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGGEEGDGFGG 725
           SRGGG+E +G GG
Sbjct: 726 SRGGGDEVEGCGG 734

BLAST of Sgr023964 vs. ExPASy TrEMBL
Match: A0A1S3CAN8 (Endo-1,3(4)-beta-glucanase OS=Cucumis melo OX=3656 GN=LOC103498567 PE=3 SV=1)

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 625/733 (85.27%), Postives = 664/733 (90.59%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSF------GNSSSKRYKL---PSPSPSLSPPPLPAMSSPSQS 60
           MLKKL+ NF++FKTLINK+F       +SSSKRYK    PSP P LSPPP P MSSPSQ 
Sbjct: 6   MLKKLKGNFKKFKTLINKTFNYSSSSSSSSSKRYKFSSSPSP-PPLSPPP-PEMSSPSQP 65

Query: 61  FRQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 120
           F QSPP  A  FPQTQS VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL
Sbjct: 66  FWQSPPPVAALFPQTQSTVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYL 125

Query: 121 IKSSLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDI 180
           IKSSLS+VS+SYPSMF NSAFG+Q+FNAD TVS      SVSQK HIIS+FSDLS+TLDI
Sbjct: 126 IKSSLSTVSISYPSMFSNSAFGFQVFNADWTVSEN--PSSVSQKPHIISSFSDLSLTLDI 185

Query: 181 PSANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYS 240
           PS NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIY+
Sbjct: 186 PSGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYA 245

Query: 241 SSPIHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYK 300
           SSPI+L H LSQITSG F GI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYK
Sbjct: 246 SSPINLTHSLSQITSGGFCGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYK 305

Query: 301 WEKKGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSV 360
           WEKKGWG+LLMLAHPLHLRLL+GD   AVVLD+FKYKSIDG LVGVVG SWVL P+PV V
Sbjct: 306 WEKKGWGNLLMLAHPLHLRLLAGDGSGAVVLDNFKYKSIDGDLVGVVGGSWVLKPDPVYV 365

Query: 361 TWHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFL 420
           TWHSINGV EEFHSEIISALVKDVEGL S+PITTT+SYFYGK+IARAARLALIAEEVN+L
Sbjct: 366 TWHSINGVGEEFHSEIISALVKDVEGLKSSPITTTASYFYGKSIARAARLALIAEEVNYL 425

Query: 421 QVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYH 480
           QVIPE+RKFLKGAIEPWL GTFNGNGFLYDGKWGG+VT+QGS DSG DFGFG+YNDHHYH
Sbjct: 426 QVIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYH 485

Query: 481 IGYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWAS 540
           IGYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWAS
Sbjct: 486 IGYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWAS 545

Query: 541 GLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREG 600
           GLTEFADGRNQES+SEAVN YYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EG
Sbjct: 546 GLTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEG 605

Query: 601 DNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFA 660
           DNLYE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSDA F 
Sbjct: 606 DNLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPLLPITEILFSDADFV 665

Query: 661 KELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIH 720
           KELVDWTLPSLGREGVGEGWKGFAYALQGI+DKDGALEKIRNL GFDDGNSLSNLLWWIH
Sbjct: 666 KELVDWTLPSLGREGVGEGWKGFAYALQGIYDKDGALEKIRNLTGFDDGNSLSNLLWWIH 725

Query: 721 SRGGGEEGDGFGG 725
           SRGGG+E +G GG
Sbjct: 726 SRGGGDEVEGCGG 734

BLAST of Sgr023964 vs. ExPASy TrEMBL
Match: A0A0A0KSJ9 (Endo-1,3(4)-beta-glucanase OS=Cucumis sativus OX=3659 GN=Csa_5G643880 PE=3 SV=1)

HSP 1 Score: 1245.7 bits (3222), Expect = 0.0e+00
Identity = 624/732 (85.25%), Postives = 663/732 (90.57%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGN-----SSSKRYKL---PSPSPSLSPPPLPAMSSPSQSF 60
           MLKKL+ NF++ +TLINKSF N     SSSKRYK    PSP P LSPPPL  MSSPSQ F
Sbjct: 1   MLKKLKGNFKKIRTLINKSFNNSSSSSSSSKRYKFSSSPSP-PPLSPPPL-EMSSPSQPF 60

Query: 61  RQSPPAAALPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 120
            QSPP  A  FPQT+S+VLPDPSNFF+PHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI
Sbjct: 61  WQSPPPVAALFPQTESSVLPDPSNFFAPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLI 120

Query: 121 KSSLSSVSVSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIP 180
           KSSLS+VSVSYPSM  NSAFGYQ+FNAD TVS      SVSQK HIIS+FSDLS+TLDIP
Sbjct: 121 KSSLSTVSVSYPSMLSNSAFGYQVFNADWTVSEN--PPSVSQKPHIISSFSDLSLTLDIP 180

Query: 181 SANLRFFLVRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSS 240
           S NLRFFLVRGSPF+TFEVFN+T ISISTIHAILSFSSNSS TKFT+TLNNNQTWLIY+S
Sbjct: 181 SGNLRFFLVRGSPFMTFEVFNNTQISISTIHAILSFSSNSSLTKFTVTLNNNQTWLIYAS 240

Query: 241 SPIHLAHGLSQITSGEFSGIIRIAALPDPRCETILDRFSSCYPVSGVAHFANPFCLEYKW 300
           SPI+L H LSQITSG F+GI+RIA LP+P CETILDRFSSCYPVSG  +F NPF LEYKW
Sbjct: 241 SPINLTHSLSQITSGGFTGIVRIAVLPNPHCETILDRFSSCYPVSGEVNFRNPFSLEYKW 300

Query: 301 EKKGWGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVT 360
           EKKGWG+LLMLAHPLHLRLLSGD  +AVVLDDFKYKSIDG LVGVVG SW L P+PV +T
Sbjct: 301 EKKGWGNLLMLAHPLHLRLLSGDGSSAVVLDDFKYKSIDGDLVGVVGGSWALKPDPVYLT 360

Query: 361 WHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQ 420
           WHSINGV EEFHSEIISALVKDVEGL S+PITTTSSYFYGK+IARAARLALIAEEVN+LQ
Sbjct: 361 WHSINGVGEEFHSEIISALVKDVEGLKSSPITTTSSYFYGKSIARAARLALIAEEVNYLQ 420

Query: 421 VIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHI 480
           VIPE+RKFLKGAIEPWL GTFNGNGFLYDGKWGG+VT+QGS DSG DFGFG+YNDHHYHI
Sbjct: 421 VIPEVRKFLKGAIEPWLLGTFNGNGFLYDGKWGGLVTQQGSHDSGGDFGFGVYNDHHYHI 480

Query: 481 GYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRRSNSSFPRLRCFDLYKLHSWASG 540
           GYFLYAIAVLVKIDPAWGRKFK  AYSLMADFMNLSRRSNS FPRLRCFDLYKLHSWASG
Sbjct: 481 GYFLYAIAVLVKIDPAWGRKFKPHAYSLMADFMNLSRRSNSMFPRLRCFDLYKLHSWASG 540

Query: 541 LTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGD 600
           LTEFADGRNQES+SEAVN YYSAALLGLAYGD HLASIGSTL ALEIKAAQTWWQI+EGD
Sbjct: 541 LTEFADGRNQESTSEAVNGYYSAALLGLAYGDTHLASIGSTLTALEIKAAQTWWQIKEGD 600

Query: 601 NLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAK 660
           NLYE DFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLP+LPITE+LFSDA F K
Sbjct: 601 NLYEEDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPILPITEILFSDADFVK 660

Query: 661 ELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHS 720
           ELVDWTLPSLGREGVGEGWKGFAYALQG++DKDGALEKIRNL GFDDGNSLSNLLWWIHS
Sbjct: 661 ELVDWTLPSLGREGVGEGWKGFAYALQGVYDKDGALEKIRNLTGFDDGNSLSNLLWWIHS 720

Query: 721 RGGGEEGDGFGG 725
           RGGG+E +  GG
Sbjct: 721 RGGGDEVEACGG 728

BLAST of Sgr023964 vs. TAIR 10
Match: AT5G15870.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 953.7 bits (2464), Expect = 8.3e-278
Identity = 476/738 (64.50%), Postives = 577/738 (78.18%), Query Frame = 0

Query: 1   MLKKLRRNFRRFKTLINKSFGNSSSKRYKLPSPSPSLSPPPLPAMSSPSQSFRQSPPAAA 60
           MLKK+R   R+ K LI K F      + + PSP P L  PP P+     Q          
Sbjct: 1   MLKKVR---RKVKVLITKPF---KKPKNRPPSPPPPLPLPPSPSPPPSQQMSSSRQKNTP 60

Query: 61  LPFPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSSVS 120
             FP++ S+VLPDPS FFS  LLSSPLPTNSFFQNFT+ NGDQ EY HPY+IK S SS+S
Sbjct: 61  FLFPRSDSSVLPDPSRFFSHDLLSSPLPTNSFFQNFTLNNGDQAEYFHPYIIKPSTSSLS 120

Query: 121 VSYPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSANLRFFL 180
           +SYPS+  NSAF Y+ FNAD+T++ +      S+KSH+IS+FSDL VTLD PS+NLRFFL
Sbjct: 121 ISYPSLSHNSAFIYEAFNADITITGSDGPDPHSRKSHLISSFSDLGVTLDFPSSNLRFFL 180

Query: 181 VRGSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSSSPIHLA-H 240
           VRGSPF+TF V  +++I+ISTIHA+LS S N+S TK+T+ LNNNQTWLIY+SSPI+L   
Sbjct: 181 VRGSPFITFSV--NSSITISTIHAVLSLSGNTSSTKYTVKLNNNQTWLIYASSPINLTKD 240

Query: 241 GLSQITSGE-FSGIIRIAALPDPR--CETILDRFSSCYPVSGVAHFANPFCLEYKWEKKG 300
           G+S I  G+ FSGIIRI  LP+P    ETILD FS  YPVSG A F  PF LEYKWEK+G
Sbjct: 241 GVSSINCGDGFSGIIRIVVLPNPNPYFETILDGFSCSYPVSGDADFTKPFALEYKWEKRG 300

Query: 301 WGDLLMLAHPLHLRLLSGDDPAAVVLDDFKYKSIDGALVGVVGSSWVLNPNPVSVTWHSI 360
           +GDLLMLAHPLHL+LLS +D +  VLD+FKY SIDG LVGV+G SWVL P+PVSVTWHSI
Sbjct: 301 YGDLLMLAHPLHLKLLSTNDCSITVLDNFKYNSIDGDLVGVIGDSWVLKPDPVSVTWHSI 360

Query: 361 NGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARAARLALIAEEVNFLQVIPE 420
            GV+E+ H EIISAL+KDV  L+S+   T SSYFY K IARAARLALIAEEV +L VIP+
Sbjct: 361 KGVQEDSHQEIISALIKDVNALDSSAEVTNSSYFYAKLIARAARLALIAEEVCYLDVIPK 420

Query: 421 LRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGADFGFGLYNDHHYHIGYFL 480
           +R +LK  IEPWL G+F  NGFLYD KWGG++TK GS DSGADFGFG+YNDHHYH+GYF+
Sbjct: 421 IRTYLKNMIEPWLNGSFGPNGFLYDPKWGGVITKLGSRDSGADFGFGIYNDHHYHLGYFV 480

Query: 481 YAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSR---RSNSSFPRLRCFDLYKLHSWASGL 540
           YAIAVL KIDP WG++++ QAY+LMAD++ L +   +SNS++PRLRCFDL+KLHSWA GL
Sbjct: 481 YAIAVLAKIDPLWGKRYRPQAYTLMADYLTLGKKGAKSNSNYPRLRCFDLFKLHSWAGGL 540

Query: 541 TEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTLVALEIKAAQTWWQIREGDN 600
           TEFADGRNQES+SEAVNAYYSAALLGLAYGD HL +  S ++ LEI AA+ WWQ++E D 
Sbjct: 541 TEFADGRNQESTSEAVNAYYSAALLGLAYGDTHLVAAASMVLTLEIHAAKMWWQVKEDDA 600

Query: 601 LYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQVLPVLPITELLFSDAGFAKE 660
           +Y  DF  EN+VVGVLWS KRDSGLWFAP +W+ECRLGIQ+LP+LP++E+LFSD  F K+
Sbjct: 601 IYPQDFTSENRVVGVLWSTKRDSGLWFAPKEWKECRLGIQLLPLLPVSEVLFSDVTFVKQ 660

Query: 661 LVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNLNGFDDGNSLSNLLWWIHSR 720
           LV+WT+P+L R+ VGEGWKGF YAL+ ++DKDGA+EKI+ LNGFDDGNSLSNLLWW+HSR
Sbjct: 661 LVNWTMPALARDSVGEGWKGFVYALESMYDKDGAMEKIKGLNGFDDGNSLSNLLWWVHSR 720

Query: 721 GGGEEGD-------GFGG 725
              ++ D       G+GG
Sbjct: 721 NNDDDDDYEEDDEGGYGG 730

BLAST of Sgr023964 vs. TAIR 10
Match: AT1G18310.1 (glycosyl hydrolase family 81 protein )

HSP 1 Score: 840.5 bits (2170), Expect = 1.0e-243
Identity = 425/679 (62.59%), Postives = 502/679 (73.93%), Query Frame = 0

Query: 63  FPQTQSAVLPDPSNFFSPHLLSSPLPTNSFFQNFTVKNGDQPEYIHPYLIKSSLSSVSVS 122
           FP+T+S+VLPDPS FFSP LLS+PLPTNSFFQNFT+KNGDQ EY HPYLIKS  SS+ +S
Sbjct: 10  FPETKSSVLPDPSRFFSPDLLSNPLPTNSFFQNFTLKNGDQAEYFHPYLIKSVDSSLCIS 69

Query: 123 YPSMFFNSAFGYQIFNADLTVSAAGISGSVSQKSHIISAFSDLSVTLDIPSANLRFFLVR 182
           YPS+F NS F Y++F AD+ +S +   G  S+K+HIIS+FSDL VTLD PS+NLRFFLVR
Sbjct: 70  YPSLFHNSDFFYEVFTADIIISGSNGPGPRSRKTHIISSFSDLVVTLDFPSSNLRFFLVR 129

Query: 183 GSPFLTFEVFNSTAISISTIHAILSFSSNSSFTKFTITLNNNQTWLIYSSSPIHLA-HGL 242
           GSP                          SS TK++  L NNQ WLIY+SSPI L  HG 
Sbjct: 130 GSP--------------------------SSSTKWSDKLTNNQRWLIYASSPIDLTKHGD 189

Query: 243 SQI-TSGEFSGIIRIAALP--DPRCETILDRFSSCYPVSGVAHFANPFCLEYKWEKKGWG 302
           S I   G F+GI+RI  LP  +P  E+ LDRFSSCYPVSG A F  PF L+Y WEK+G G
Sbjct: 190 SSIHCRGGFTGIVRITVLPGSNPGYESTLDRFSSCYPVSGDADFTKPFTLKYMWEKRGSG 249

Query: 303 DLLMLAHPLHLRLLSGD--------------------DPAAVVLDDFKYKSIDGALVGVV 362
           DLLMLAHPLHL+LL+ D                    + +  VLD F+YKSIDG LVGVV
Sbjct: 250 DLLMLAHPLHLKLLAKDASSLFQQSTLLLVCPDRGCTNSSVTVLDHFRYKSIDGDLVGVV 309

Query: 363 GSSWVLNPNPVSVTWHSINGVEEEFHSEIISALVKDVEGLNSAPITTTSSYFYGKAIARA 422
           G SWVL P+ VSVTWHS+ GV+++ + EIISAL KDV GLNS+   T+SSYFYGK IARA
Sbjct: 310 GDSWVLKPDSVSVTWHSMKGVKKDSYKEIISALGKDVNGLNSSAEVTSSSYFYGKLIARA 369

Query: 423 ARLALIAEEVNFLQVIPELRKFLKGAIEPWLRGTFNGNGFLYDGKWGGIVTKQGSSDSGA 482
           AR ALIAEEV +L VIP++  +LK  IEPWL G+F  NGFLYD KWGG++TKQGS DS A
Sbjct: 370 ARFALIAEEVCYLDVIPKIVTYLKNMIEPWLDGSFKPNGFLYDPKWGGLITKQGSKDSQA 429

Query: 483 DFGFGLYNDHHYHIGYFLYAIAVLVKIDPAWGRKFKRQAYSLMADFMNLSRR----SNSS 542
           DFGFG+YNDHHYHIGYFLYAIAVL K DP WG +++ QAYSL+ADFM   R+    SNSS
Sbjct: 430 DFGFGIYNDHHYHIGYFLYAIAVLAKFDPLWGERYRAQAYSLLADFMTFGRKDDNNSNSS 489

Query: 543 FPRLRCFDLYKLHSWASGLTEFADGRNQESSSEAVNAYYSAALLGLAYGDIHLASIGSTL 602
           +PRLR FDL+KLHSWA GLTEF DGRNQES+SEAVNAYYSAALLGLAYGD HL    ST+
Sbjct: 490 YPRLRNFDLFKLHSWAGGLTEFWDGRNQESTSEAVNAYYSAALLGLAYGDKHLVETASTI 549

Query: 603 VALEIKAAQTWWQIREGDNLYEVDFARENKVVGVLWSNKRDSGLWFAPPDWRECRLGIQV 662
           + LEI AA+ WWQ+++G+ LY  DF  EN+VVGVLWS KRDS LWF P +W+ECRLGIQ+
Sbjct: 550 MTLEIHAAKMWWQVKKGEALYPKDFTAENRVVGVLWSTKRDSSLWFGPKEWKECRLGIQL 609

Query: 663 LPVLPITELLFSDAGFAKELVDWTLPSLGREGVGEGWKGFAYALQGIFDKDGALEKIRNL 714
           LP+LP              LV+WTLP+L R GVGEGWKGF YAL+ ++DKDGA++KI+ L
Sbjct: 610 LPILP--------------LVNWTLPALQRNGVGEGWKGFLYALESLYDKDGAIKKIKRL 648