Sgr023949 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023949 vs. NCBI nr
Match: XP_038890905.1 (cytokinin hydroxylase-like [Benincasa hispida])

HSP 1 Score: 961.1 bits (2483), Expect = 4.0e-276
Identity = 473/517 (91.49%), Postives = 493/517 (95.36%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +LLTTL    TS FIFS+ YDT+SCY  TPRRIK  MAKQGVRGPKPRPLLGNILDV
Sbjct: 4   MLPLLLTTLVLLTTSFFIFSLLYDTLSCYCFTPRRIKHKMAKQGVRGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           AALLSKSTS+DMP+ISHD+VPRLLPHFL+WS+QFGKRFIFWNGIEPRMCLTETDLIKELL
Sbjct: 64  AALLSKSTSSDMPSISHDLVPRLLPHFLAWSTQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           +EML+SIENEV SGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ
Sbjct: 184 REMLESIENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLCLPGSRFFPSKYNREIKALK KVE LLMEIIQSRRDCVE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLA+NPSWQHK+RAEV+
Sbjct: 304 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPSWQHKLRAEVI 363

Query: 363 AVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 422
           A+C   T S  HLSKLSLL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP
Sbjct: 364 AICGSETPSFHHLSKLSLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 423

Query: 423 VLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 482
           VLAIHHSEE+WGKDANEFNPERFANSKAFTSG FIPFASGPRNCVGQSFALMETKIILAM
Sbjct: 424 VLAIHHSEEIWGKDANEFNPERFANSKAFTSGGFIPFASGPRNCVGQSFALMETKIILAM 483

Query: 483 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           LISKFSFTISD YRHAPVVVLTIKPKYGVQVCLKP N
Sbjct: 484 LISKFSFTISDKYRHAPVVVLTIKPKYGVQVCLKPLN 520

BLAST of Sgr023949 vs. NCBI nr
Match: XP_011656036.1 (cytokinin hydroxylase [Cucumis sativus] >KAE8648965.1 hypothetical protein Csa_008948 [Cucumis sativus])

HSP 1 Score: 948.0 bits (2449), Expect = 3.5e-272
Identity = 472/515 (91.65%), Postives = 491/515 (95.34%), Query Frame = 0

Query: 7   LLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDVAALL 66
           LLTTLA   TS FIFS+ YDT+SCY  TPRRIK+ MAKQGV GPKPRPLLGNILDVA+LL
Sbjct: 8   LLTTLALLTTSFFIFSLLYDTLSCYLFTPRRIKQKMAKQGVHGPKPRPLLGNILDVASLL 67

Query: 67  SKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYS 126
           SKSTS+DMP+ISHD+VPRLLPHFL+WSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYS
Sbjct: 68  SKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYS 127

Query: 127 AVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECTKEML 186
           AVSGKSWLQQQGSKHFIGRGLLMANGQNW+HQRHIVAPAF+GDRLKSYAGYMVECTKEML
Sbjct: 128 AVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECTKEML 187

Query: 187 QSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRH 246
           +S+E EV SGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQASRH
Sbjct: 188 ESLEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQASRH 247

Query: 247 LCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLLNEMQ 306
           LCLPGSRFFPSKYNREIKALK KVE LLMEIIQSRRDCVE+GRSSSYGNDLLGMLLNEMQ
Sbjct: 248 LCLPGSRFFPSKYNREIKALKGKVEELLMEIIQSRRDCVEIGRSSSYGNDLLGMLLNEMQ 307

Query: 307 KKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAV 366
           KKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLA+NP+WQ+KVRAEVMAV
Sbjct: 308 KKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQNKVRAEVMAV 367

Query: 367 CNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 426
           C   T S  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL
Sbjct: 368 CGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVL 427

Query: 427 AIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAMLI 486
           AIHHSEELWGKDANEFNPERFANSK FTSG FIPFASGPRNCVGQSFALMETKIILAMLI
Sbjct: 428 AIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCVGQSFALMETKIILAMLI 487

Query: 487 SKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           SKFSFTISD+YRHAPVVVLTIKPKYGVQVCL+P N
Sbjct: 488 SKFSFTISDSYRHAPVVVLTIKPKYGVQVCLEPLN 522

BLAST of Sgr023949 vs. NCBI nr
Match: KAA0039372.1 (cytokinin hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 948.0 bits (2449), Expect = 3.5e-272
Identity = 472/519 (90.94%), Postives = 492/519 (94.80%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +LLTTLA   TS FIFS+ YDT+SCY LTPRRIK+ MAKQGV GPKPRPLLGNILDV
Sbjct: 4   MLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           A+LLSKSTS+DMP+ISHD+VPRLLPHFL+WSSQFGKRFIFWNGIEPRMCLTETDLIKELL
Sbjct: 64  ASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           KEML+SIE EV SGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQ
Sbjct: 184 KEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLC PGSRFFPSKYNREIKALK KVE LLMEIIQSR+DCVE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAE 362
           NEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLA+NP+WQ KVRAE
Sbjct: 304 NEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAE 363

Query: 363 VMAVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
           VMAVC   T S  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW
Sbjct: 364 VMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 423

Query: 423 IPVLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIIL 482
           IPVLAIHHSEELWGKDANEFNPERFANSK FTSG FIPFASGPRNC+GQSFALMETKIIL
Sbjct: 424 IPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIIL 483

Query: 483 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP N
Sbjct: 484 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Sgr023949 vs. NCBI nr
Match: XP_008459472.1 (PREDICTED: cytokinin hydroxylase-like [Cucumis melo])

HSP 1 Score: 946.8 bits (2446), Expect = 7.8e-272
Identity = 471/519 (90.75%), Postives = 492/519 (94.80%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +LLTTLA   TS FIFS+ YDT+SCY LTPRRIK+ MAKQGV GPKPRPLLGNILDV
Sbjct: 4   MLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           A+LLSKSTS+DMP+ISHD+VPRLLPHFL+WSSQFGKRFIFWNGIEPRMCLTETDLIKELL
Sbjct: 64  ASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           KEML+SIE EV SGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQ
Sbjct: 184 KEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLC PGSRFFPSKYNREIKALK KVE LLMEIIQSR+DCVE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAE 362
           NEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLA+NP+WQ KVRAE
Sbjct: 304 NEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAE 363

Query: 363 VMAVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
           VMAVC   T S  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW
Sbjct: 364 VMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 423

Query: 423 IPVLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIIL 482
           IP+LAIHHSEELWGKDANEFNPERFANSK FTSG FIPFASGPRNC+GQSFALMETKIIL
Sbjct: 424 IPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIIL 483

Query: 483 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP N
Sbjct: 484 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Sgr023949 vs. NCBI nr
Match: XP_023551159.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 937.6 bits (2422), Expect = 4.7e-269
Identity = 458/516 (88.76%), Postives = 491/516 (95.16%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++A++LTTLAFAATS  IFS+ Y T+SCYWLTPRRI + MAKQGVRGPKPRPLLGNILDV
Sbjct: 4   MLALVLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           AALLSKSTS+DMP+ISHD+VPRLLPH+L+WS+QFGKRFIFWNGIEPRMCLTET LIKELL
Sbjct: 64  AALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           +EMLQS+ENEV SGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ
Sbjct: 184 REMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLCLPGSRFFPSKYNREIKALK KVEGLLMEIIQSR+D VE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLA+NPSWQ +VRAE+ 
Sbjct: 304 NEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAELN 363

Query: 363 AVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 422
           AVCN  T SL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+WIP
Sbjct: 364 AVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVWIP 423

Query: 423 VLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 482
           V+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKIILAM
Sbjct: 424 VVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIILAM 483

Query: 483 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPF 519
           LISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL PF
Sbjct: 484 LISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTPF 519

BLAST of Sgr023949 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 4.5e-214
Identity = 366/517 (70.79%), Postives = 433/517 (83.75%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +L + L    T+  I  V YDTISCYWLTPRRIKK M +QGV GPKPRPL GNIL++
Sbjct: 2   LLTILKSLLVIFVTT--ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI 61

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           +A++S+S S D  +I HDIV RLLPH+++WS Q+GKRFI WNG +PR+CLTET+LIKELL
Sbjct: 62  SAMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELL 121

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
            K++ VSG+SWLQQQG+K+FIGRGLLMANGQ+W+HQRH+ APAF G+RLK YA +MVECT
Sbjct: 122 MKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECT 181

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
            ++++ +  EV  G +E EIGE M +LTADIISRT+F SS+EKGK++F+ LTVLQ  CAQ
Sbjct: 182 SKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQ 241

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           A+RHLC PGSRF PSKYNREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+LL
Sbjct: 242 ATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLL 301

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEM   K+ N  + NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ KVR EV 
Sbjct: 302 NEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVR 361

Query: 363 AVC-NGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 422
            V    G  S++ LSKL+ LS VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWI
Sbjct: 362 EVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWI 421

Query: 423 PVLAIHHSEELWGKDANEFNPERFANSKAFTSGR-FIPFASGPRNCVGQSFALMETKIIL 482
           PVLAIHHSEELWGKDAN+FNPERF   + F SGR FIPFA+GPRNC+GQ FALME KIIL
Sbjct: 422 PVLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIIL 481

Query: 483 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP 518
           A LISKF+FTIS NYRHAP+VVLTIKPKYGVQV LKP
Sbjct: 482 ATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKP 515

BLAST of Sgr023949 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 9.1e-207
Identity = 359/520 (69.04%), Postives = 421/520 (80.96%), Query Frame = 0

Query: 1   MAIVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNIL 60
           M +  VL   L    T   I  V YD+I CY+LTPRRIKK M +QG+ GPKPR L GNI+
Sbjct: 1   MMVTLVLKYVLVIVMT--LILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNII 60

Query: 61  DVAALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKE 120
           D++ +LS S S D  +I H+IVPRLLPH++SWS Q+GKRFI WNG EPR+CLTET++IKE
Sbjct: 61  DISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKE 120

Query: 121 LLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVE 180
           LL+K++ V+GKSWLQQQG+K FIGRGLLMANG+ W+HQRH+ APAF  DRLK YA +MVE
Sbjct: 121 LLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 180

Query: 181 CTKEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 240
           CTK M + +  EV     E EIGE M RLTADIISRTEF SS +KGK++F LLTVLQ LC
Sbjct: 181 CTKMMAERLRKEVG---EEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 240

Query: 241 AQASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGM 300
           AQA+RHLC PGSRF PSKYNREIK+LKT+VE LLMEII SR+D VE+GRSSSYG+DLLG+
Sbjct: 241 AQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGL 300

Query: 301 LLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAE 360
           LLN+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  VR E
Sbjct: 301 LLNQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDE 360

Query: 361 VMAVC-NGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           V  VC   G  S+E LS L+ L+ VINESLRLYPPA++LPRMAFEDIKLGDL IPKGLSI
Sbjct: 361 VRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPVLAIHHS ELWG+DANEFNPERF      +S  F+PFA+GPRNC+GQ+FA+ME KII
Sbjct: 421 WIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           LAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKP +
Sbjct: 481 LAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Sgr023949 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 372.1 bits (954), Expect = 1.0e-101
Identity = 203/499 (40.68%), Postives = 293/499 (58.72%), Query Frame = 0

Query: 32  WLTPRRIKKTMAKQGVRGPKPRPLLGNILDVAALLSKSTSADMPAISHDIVPRLLPHFLS 91
           W+  RR K    KQG+ GPK R L GN+ ++  + +++    +   S+DIVPR+LPH   
Sbjct: 89  WMLSRRFK----KQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQ 148

Query: 92  WSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMAN 151
           W SQ+G+ F++W G +PR+C+++ +L K++LS       KS  + +  K   G GL+  N
Sbjct: 149 WKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVN 208

Query: 152 GQNWYHQRHIVAPAFMGDRLKSYAGYMVECTKEMLQSIENEVNSGRSE--FEIGEYMTRL 211
           G +W   R I+ PAF  D+LK     MV+CT  M    + + N   +E    I     RL
Sbjct: 209 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 268

Query: 212 TADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKTK 271
           TADII+   F SSY +G ++F     LQ  CA A   L  PG ++ P+  N +I  L  K
Sbjct: 269 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMK 328

Query: 272 VEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL-----NEMQKKKDGNGLSLNLQIIMDE 331
           V   +  II +R        S  YGNDLLG++L     NE +KK       +++  I++E
Sbjct: 329 VNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNESEKK-------MSIDEIIEE 388

Query: 332 CKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAVCNGGTL-SLEHLSKLSLLSM 391
           CKTFFFAGHETTA LLTW+ MLL+ +  WQ K+R EV   C    +   E  SKL L++ 
Sbjct: 389 CKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNT 448

Query: 392 VINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPE 451
           V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+  +H  + +WG DA++FNP 
Sbjct: 449 VFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPM 508

Query: 452 RFANSKAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYRHAPV 511
           RFAN  +  +      + F+ GPR C+GQ+FA+ME K +LAM++ +F   +S +Y+HAP 
Sbjct: 509 RFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPA 568

Query: 512 VVLTIKPKYGVQVCLKPFN 520
             LT++P+Y + V L+P +
Sbjct: 569 DHLTLQPQYDLPVILEPID 571

BLAST of Sgr023949 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 8.2e-99
Identity = 192/493 (38.95%), Postives = 292/493 (59.23%), Query Frame = 0

Query: 35  PRRIKKTMAKQGVRGPKPRPLLGNILDVAALLSKSTSADMPAISHDIVPRLLPHFLSWSS 94
           P  + + +  QG+ GP  R   GN+ ++  +  +S  + +   S+DI PR+LPH+  W S
Sbjct: 31  PFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMS 90

Query: 95  QFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQN 154
           Q+G+ F++WNG EPR+C+++ +L K +LS       KS  + +  K    +GL+   G +
Sbjct: 91  QYGETFLYWNGTEPRICISDPELAKTMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGAD 150

Query: 155 WYHQRHIVAPAFMGDRLKSYAGYMVECTKEML-----QSIENEVNSGRSEFEIGEYMTRL 214
           W   R I+ PAF  DRLK     MV+CT +ML     +S + E    + + E+ E   RL
Sbjct: 151 WVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRL 210

Query: 215 TADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKTK 274
           TADII+ + F SSY +G ++F     L+     +   + +PG+++ P+  N  +  L+ K
Sbjct: 211 TADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERK 270

Query: 275 VEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFF 334
           ++  +  II SR     +   S YG+DLLG+LL       +G    ++++ I+ EC+TFF
Sbjct: 271 MDNSIKRIISSR-----LQSKSDYGDDLLGILLKAY--NTEGKERKMSIEEIIHECRTFF 330

Query: 335 FAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAVC-NGGTLSLEHLSKLSLLSMVINES 394
           F GHETT+ LL WT MLL+ +  WQ K+R E+   C    T   E  SKL L++MVI ES
Sbjct: 331 FGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMES 390

Query: 395 LRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPERFANS 454
           LRLY P S L R A  +IKLGDLEIPKG ++ IP+L +H  + LWG DA++FNP RFAN 
Sbjct: 391 LRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANG 450

Query: 455 KAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSF-TISDNYRHAPVVVLT 514
            +  +      + F+ GPR C+GQ+F ++E K +L M++ +F F ++ D Y+H PV  +T
Sbjct: 451 VSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVT 510

Query: 515 IKPKYGVQVCLKP 518
           I+P+YG+ V L+P
Sbjct: 511 IQPQYGLPVMLQP 516

BLAST of Sgr023949 vs. ExPASy Swiss-Prot
Match: Q2QYH7 (Cytochrome P450 714C2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP714C2 PE=2 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 2.5e-95
Identity = 197/513 (38.40%), Postives = 292/513 (56.92%), Query Frame = 0

Query: 18  VFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDVAAL--LSKST----- 77
           + +FS  Y  +   WL P R+++ +  QGVRGPKP  L GNI ++  +  L+KS      
Sbjct: 20  ILLFSYVYIIL---WLRPERLRQKLRSQGVRGPKPSFLFGNIPEMRRIQQLAKSAHEQEA 79

Query: 78  -SADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVS 137
            S DM   S + V  L P+FL WS  +G  +++  G    + +T+ +++KEL +  S   
Sbjct: 80  GSTDM--FSSNYVATLFPYFLHWSRVYGSIYLYSTGSIQVLNVTDPNMVKELANCKSLDL 139

Query: 138 GKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECTKEMLQSI 197
           GK    Q+     +G G+L +NG  W HQR ++AP    +R+K     M+E    ML S 
Sbjct: 140 GKPCYLQKERGALLGMGILTSNGDLWVHQRKVIAPELFMERVKGMVNLMMEAAMSMLNSW 199

Query: 198 ENEV--NSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHL 257
           +NEV    G +E  + E++   +AD+ISR  F SS+ +GK+IF  +  LQ   A+ S  +
Sbjct: 200 KNEVEDRGGSAEIVVDEFLRTFSADVISRACFGSSFSEGKEIFIKIRQLQKAMAKQSMLI 259

Query: 258 CLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLLNEMQK 317
            +PGSR+ P++ NR I  L + +  L++ I +      E   S+S   DLL  ++   Q 
Sbjct: 260 GVPGSRYLPTRSNRGIWNLDSSIRTLILNISKK----YEHDSSTSVNKDLLHSII---QG 319

Query: 318 KKDGNGLSLNLQ-IIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAVCN 377
            KDG   S   +  I+D CK  +FAGHETT+    W +MLLAS+  WQ + R E + +C 
Sbjct: 320 SKDGPFASCTPEDFIVDNCKNIYFAGHETTSTTAAWCLMLLASHHEWQSRARVESLDICQ 379

Query: 378 GGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAI 437
           G  L  + L KL  L+MVI E+LRLYPPAS + R A  D+KLG ++IPKG +IWIP+   
Sbjct: 380 GRPLDFDILRKLKKLTMVIQETLRLYPPASFVAREALNDMKLGGIDIPKGTNIWIPIAMA 439

Query: 438 HHSEELWGKDANEFNPERFANSKAFT---SGRFIPFASGPRNCVGQSFALMETKIILAML 497
           H    +WG  A++F+P+RFAN  A        ++PF  G R C GQ+ A++E K++L++L
Sbjct: 440 HRDPSVWGPSADKFDPDRFANGIAGACKPPHMYMPFGVGVRTCAGQNLAMVELKVVLSLL 499

Query: 498 ISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLK 517
           +SKF F +S NY H P   LTI+P  GV +  +
Sbjct: 500 LSKFEFKLSPNYVHCPAFRLTIEPGKGVPLIFR 520

BLAST of Sgr023949 vs. ExPASy TrEMBL
Match: A0A5A7T7B8 (Cytokinin hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold64G001740 PE=3 SV=1)

HSP 1 Score: 948.0 bits (2449), Expect = 1.7e-272
Identity = 472/519 (90.94%), Postives = 492/519 (94.80%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +LLTTLA   TS FIFS+ YDT+SCY LTPRRIK+ MAKQGV GPKPRPLLGNILDV
Sbjct: 4   MLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           A+LLSKSTS+DMP+ISHD+VPRLLPHFL+WSSQFGKRFIFWNGIEPRMCLTETDLIKELL
Sbjct: 64  ASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           KEML+SIE EV SGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQ
Sbjct: 184 KEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLC PGSRFFPSKYNREIKALK KVE LLMEIIQSR+DCVE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAE 362
           NEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLA+NP+WQ KVRAE
Sbjct: 304 NEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAE 363

Query: 363 VMAVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
           VMAVC   T S  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW
Sbjct: 364 VMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 423

Query: 423 IPVLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIIL 482
           IPVLAIHHSEELWGKDANEFNPERFANSK FTSG FIPFASGPRNC+GQSFALMETKIIL
Sbjct: 424 IPVLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIIL 483

Query: 483 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP N
Sbjct: 484 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Sgr023949 vs. ExPASy TrEMBL
Match: A0A1S3CAS2 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103498598 PE=3 SV=1)

HSP 1 Score: 946.8 bits (2446), Expect = 3.8e-272
Identity = 471/519 (90.75%), Postives = 492/519 (94.80%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +LLTTLA   TS FIFS+ YDT+SCY LTPRRIK+ MAKQGV GPKPRPLLGNILDV
Sbjct: 4   MLPLLLTTLALLTTSFFIFSLLYDTVSCYLLTPRRIKQKMAKQGVHGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           A+LLSKSTS+DMP+ISHD+VPRLLPHFL+WSSQFGKRFIFWNGIEPRMCLTETDLIKELL
Sbjct: 64  ASLLSKSTSSDMPSISHDLVPRLLPHFLAWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS VSGKSWLQQQGSKHFIGRGLLMANGQNW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSTVSGKSWLQQQGSKHFIGRGLLMANGQNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           KEML+SIE EV SGRSEFEIGEYMTRLTADIISRTEFESS+EKGKQIFHLLTVLQHLCAQ
Sbjct: 184 KEMLESIEKEVKSGRSEFEIGEYMTRLTADIISRTEFESSFEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLC PGSRFFPSKYNREIKALK KVE LLMEIIQSR+DCVE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCFPGSRFFPSKYNREIKALKGKVEELLMEIIQSRKDCVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKK-DG-NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAE 362
           NEMQKKK DG NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLA+NP+WQ KVRAE
Sbjct: 304 NEMQKKKLDGNNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLATNPTWQDKVRAE 363

Query: 363 VMAVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 422
           VMAVC   T S  HLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW
Sbjct: 364 VMAVCGSETPSFHHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIW 423

Query: 423 IPVLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIIL 482
           IP+LAIHHSEELWGKDANEFNPERFANSK FTSG FIPFASGPRNC+GQSFALMETKIIL
Sbjct: 424 IPLLAIHHSEELWGKDANEFNPERFANSKPFTSGGFIPFASGPRNCIGQSFALMETKIIL 483

Query: 483 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP N
Sbjct: 484 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPLN 522

BLAST of Sgr023949 vs. ExPASy TrEMBL
Match: A0A6J1FGF0 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 936.4 bits (2419), Expect = 5.1e-269
Identity = 458/515 (88.93%), Postives = 490/515 (95.15%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++A+LLTTLAFAATS  IFS+ Y T+SCYWLTPRRI + MAKQGVRGPKPRPLLGNILDV
Sbjct: 4   MLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           AALLSKSTS+DMP+ISHD+VPRLLPH+L+WS+QFGKRFIFWNGIEPRMCLTET LIKELL
Sbjct: 64  AALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           +EMLQS+ENEV SGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ
Sbjct: 184 REMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLCLPGSRFFPSKYNREIKALK KVEGLLMEIIQSR+D VE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLA+NPSWQ +VRAE+ 
Sbjct: 304 NEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAELN 363

Query: 363 AVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 422
           AVCN  T SL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+WIP
Sbjct: 364 AVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVWIP 423

Query: 423 VLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 482
           V+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKIILAM
Sbjct: 424 VVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIILAM 483

Query: 483 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP 518
           LISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL P
Sbjct: 484 LISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTP 518

BLAST of Sgr023949 vs. ExPASy TrEMBL
Match: A0A6J1JYD2 (cytokinin hydroxylase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111488993 PE=3 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 1.5e-268
Identity = 457/515 (88.74%), Postives = 489/515 (94.95%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++A+LLTTLAFA TS  IFS+ Y T+SCYWLTPRRI + MAKQGVRGPKPRPLLGNILDV
Sbjct: 4   MLALLLTTLAFAVTSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           AALLSKSTS+DMP+ISHD+VPRLLPH+L+WS+QFGKRFIFWNGIEPRMCLTET LIKELL
Sbjct: 64  AALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           +EMLQS+ENEV SGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ
Sbjct: 184 REMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLCLPGSRFFPSKYNREIKALK KVEGLLMEIIQSR+D VE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCLPGSRFFPSKYNREIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLA+NPSWQ +VRAE+ 
Sbjct: 304 NEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAELN 363

Query: 363 AVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 422
           AVCN  T SL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+WIP
Sbjct: 364 AVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVWIP 423

Query: 423 VLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 482
           V+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKIILAM
Sbjct: 424 VVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIILAM 483

Query: 483 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP 518
           LISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL P
Sbjct: 484 LISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTP 518

BLAST of Sgr023949 vs. ExPASy TrEMBL
Match: A0A6J1FHA5 (cytokinin hydroxylase-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111445453 PE=3 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.3e-261
Identity = 450/515 (87.38%), Postives = 482/515 (93.59%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++A+LLTTLAFAATS  IFS+ Y T+SCYWLTPRRI + MAKQGVRGPKPRPLLGNILDV
Sbjct: 4   MLALLLTTLAFAATSFCIFSLLYHTLSCYWLTPRRIMRKMAKQGVRGPKPRPLLGNILDV 63

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           AALLSKSTS+DMP+ISHD+VPRLLPH+L+WS+QFGKRFIFWNGIEPRMCLTET LIKELL
Sbjct: 64  AALLSKSTSSDMPSISHDLVPRLLPHYLAWSAQFGKRFIFWNGIEPRMCLTETHLIKELL 123

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
           SKYS+VSGKSWLQQQGSKHFIGRGLLMANG NW+HQRHIVAPAF+GDRLKSYAGYMVECT
Sbjct: 124 SKYSSVSGKSWLQQQGSKHFIGRGLLMANGPNWFHQRHIVAPAFVGDRLKSYAGYMVECT 183

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
           +EMLQS+ENEV SGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ
Sbjct: 184 REMLQSLENEVKSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 243

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           ASRHLCLPGS        REIKALK KVEGLLMEIIQSR+D VE+GRSSSYGNDLLGMLL
Sbjct: 244 ASRHLCLPGS--------REIKALKGKVEGLLMEIIQSRKDGVEIGRSSSYGNDLLGMLL 303

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEMQKKK+GNGLSLNLQIIMDECKTFFFAGHETTALLLTWT+MLLA+NPSWQ +VRAE+ 
Sbjct: 304 NEMQKKKNGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTIMLLATNPSWQDRVRAELN 363

Query: 363 AVCNGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIP 422
           AVCN  T SL HLSKL LL MVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLS+WIP
Sbjct: 364 AVCNSQTPSLHHLSKLPLLGMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSVWIP 423

Query: 423 VLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKIILAM 482
           V+AIHHSEE+WGKDANEFNPERF +SKAFT G FIPFASGPRNCVGQ+FALMETKIILAM
Sbjct: 424 VVAIHHSEEIWGKDANEFNPERFGSSKAFTCGGFIPFASGPRNCVGQAFALMETKIILAM 483

Query: 483 LISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP 518
           LISKF FTIS +YRHAPVVVLT+KPKYGVQ+CL P
Sbjct: 484 LISKFRFTISPSYRHAPVVVLTVKPKYGVQICLTP 510

BLAST of Sgr023949 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 745.3 bits (1923), Expect = 3.2e-215
Identity = 366/517 (70.79%), Postives = 433/517 (83.75%), Query Frame = 0

Query: 3   IVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDV 62
           ++ +L + L    T+  I  V YDTISCYWLTPRRIKK M +QGV GPKPRPL GNIL++
Sbjct: 2   LLTILKSLLVIFVTT--ILRVLYDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEI 61

Query: 63  AALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELL 122
           +A++S+S S D  +I HDIV RLLPH+++WS Q+GKRFI WNG +PR+CLTET+LIKELL
Sbjct: 62  SAMVSQSASKDCDSIHHDIVGRLLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELL 121

Query: 123 SKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECT 182
            K++ VSG+SWLQQQG+K+FIGRGLLMANGQ+W+HQRH+ APAF G+RLK YA +MVECT
Sbjct: 122 MKHNGVSGRSWLQQQGTKNFIGRGLLMANGQDWHHQRHLAAPAFTGERLKGYARHMVECT 181

Query: 183 KEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQ 242
            ++++ +  EV  G +E EIGE M +LTADIISRT+F SS+EKGK++F+ LTVLQ  CAQ
Sbjct: 182 SKLVERLRKEVGEGANEVEIGEEMHKLTADIISRTKFGSSFEKGKELFNHLTVLQRRCAQ 241

Query: 243 ASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL 302
           A+RHLC PGSRF PSKYNREIK+LK +VE LL+EIIQSRRDC E+GRSS++G+DLLG+LL
Sbjct: 242 ATRHLCFPGSRFLPSKYNREIKSLKKEVERLLIEIIQSRRDCAEMGRSSTHGDDLLGLLL 301

Query: 303 NEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVM 362
           NEM   K+ N  + NLQ+IMDECKTFFFAGHETTALLLTWT MLLA NP+WQ KVR EV 
Sbjct: 302 NEMDIDKNNNNNNNNLQLIMDECKTFFFAGHETTALLLTWTTMLLADNPTWQEKVREEVR 361

Query: 363 AVC-NGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWI 422
            V    G  S++ LSKL+ LS VINESLRLYPPA++LPRMAFED+KLGDL IPKGLSIWI
Sbjct: 362 EVFGRNGLPSVDQLSKLTSLSKVINESLRLYPPATLLPRMAFEDLKLGDLTIPKGLSIWI 421

Query: 423 PVLAIHHSEELWGKDANEFNPERFANSKAFTSGR-FIPFASGPRNCVGQSFALMETKIIL 482
           PVLAIHHSEELWGKDAN+FNPERF   + F SGR FIPFA+GPRNC+GQ FALME KIIL
Sbjct: 422 PVLAIHHSEELWGKDANQFNPERF-GGRPFASGRHFIPFAAGPRNCIGQQFALMEAKIIL 481

Query: 483 AMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKP 518
           A LISKF+FTIS NYRHAP+VVLTIKPKYGVQV LKP
Sbjct: 482 ATLISKFNFTISKNYRHAPIVVLTIKPKYGVQVILKP 515

BLAST of Sgr023949 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 721.1 bits (1860), Expect = 6.4e-208
Identity = 359/520 (69.04%), Postives = 421/520 (80.96%), Query Frame = 0

Query: 1   MAIVAVLLTTLAFAATSVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNIL 60
           M +  VL   L    T   I  V YD+I CY+LTPRRIKK M +QG+ GPKPR L GNI+
Sbjct: 1   MMVTLVLKYVLVIVMT--LILRVLYDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNII 60

Query: 61  DVAALLSKSTSADMPAISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKE 120
           D++ +LS S S D  +I H+IVPRLLPH++SWS Q+GKRFI WNG EPR+CLTET++IKE
Sbjct: 61  DISKMLSHSASNDCSSIHHNIVPRLLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKE 120

Query: 121 LLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVE 180
           LL+K++ V+GKSWLQQQG+K FIGRGLLMANG+ W+HQRH+ APAF  DRLK YA +MVE
Sbjct: 121 LLTKHNPVTGKSWLQQQGTKGFIGRGLLMANGEAWHHQRHMAAPAFTRDRLKGYAKHMVE 180

Query: 181 CTKEMLQSIENEVNSGRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLC 240
           CTK M + +  EV     E EIGE M RLTADIISRTEF SS +KGK++F LLTVLQ LC
Sbjct: 181 CTKMMAERLRKEVG---EEVEIGEEMRRLTADIISRTEFGSSCDKGKELFSLLTVLQRLC 240

Query: 241 AQASRHLCLPGSRFFPSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSSYGNDLLGM 300
           AQA+RHLC PGSRF PSKYNREIK+LKT+VE LLMEII SR+D VE+GRSSSYG+DLLG+
Sbjct: 241 AQATRHLCFPGSRFLPSKYNREIKSLKTEVERLLMEIIDSRKDSVEIGRSSSYGDDLLGL 300

Query: 301 LLNEMQKKKDGNGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAE 360
           LLN+M    D N  +LN+Q+IMDECKTFFF GHETT+LLLTWT+MLLA NP+WQ  VR E
Sbjct: 301 LLNQM----DSNKNNLNVQMIMDECKTFFFTGHETTSLLLTWTLMLLAHNPTWQDNVRDE 360

Query: 361 VMAVC-NGGTLSLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSI 420
           V  VC   G  S+E LS L+ L+ VINESLRLYPPA++LPRMAFEDIKLGDL IPKGLSI
Sbjct: 361 VRQVCGQDGVPSVEQLSSLTSLNKVINESLRLYPPATLLPRMAFEDIKLGDLIIPKGLSI 420

Query: 421 WIPVLAIHHSEELWGKDANEFNPERFANSKAFTSGRFIPFASGPRNCVGQSFALMETKII 480
           WIPVLAIHHS ELWG+DANEFNPERF      +S  F+PFA+GPRNC+GQ+FA+ME KII
Sbjct: 421 WIPVLAIHHSNELWGEDANEFNPERFTTRSFASSRHFMPFAAGPRNCIGQTFAMMEAKII 480

Query: 481 LAMLISKFSFTISDNYRHAPVVVLTIKPKYGVQVCLKPFN 520
           LAML+SKFSF IS+NYRHAP+VVLTIKPKYGVQ+ LKP +
Sbjct: 481 LAMLVSKFSFAISENYRHAPIVVLTIKPKYGVQLVLKPLD 511

BLAST of Sgr023949 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 372.1 bits (954), Expect = 7.3e-103
Identity = 203/499 (40.68%), Postives = 293/499 (58.72%), Query Frame = 0

Query: 32  WLTPRRIKKTMAKQGVRGPKPRPLLGNILDVAALLSKSTSADMPAISHDIVPRLLPHFLS 91
           W+  RR K    KQG+ GPK R L GN+ ++  + +++    +   S+DIVPR+LPH   
Sbjct: 89  WMLSRRFK----KQGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQ 148

Query: 92  WSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMAN 151
           W SQ+G+ F++W G +PR+C+++ +L K++LS       KS  + +  K   G GL+  N
Sbjct: 149 WKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVN 208

Query: 152 GQNWYHQRHIVAPAFMGDRLKSYAGYMVECTKEMLQSIENEVNSGRSE--FEIGEYMTRL 211
           G +W   R I+ PAF  D+LK     MV+CT  M    + + N   +E    I     RL
Sbjct: 209 GLDWVRHRRILNPAFSMDKLKLMTQLMVDCTFRMFLEWKKQRNGVETEQFVLISREFKRL 268

Query: 212 TADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKTK 271
           TADII+   F SSY +G ++F     LQ  CA A   L  PG ++ P+  N +I  L  K
Sbjct: 269 TADIIATAAFGSSYAEGIEVFKSQLELQKCCAAALTDLYFPGIQYLPTPSNLQIWKLDMK 328

Query: 272 VEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLL-----NEMQKKKDGNGLSLNLQIIMDE 331
           V   +  II +R        S  YGNDLLG++L     NE +KK       +++  I++E
Sbjct: 329 VNSSIKRIIDARL----TSESKDYGNDLLGIMLTAASSNESEKK-------MSIDEIIEE 388

Query: 332 CKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAVCNGGTL-SLEHLSKLSLLSM 391
           CKTFFFAGHETTA LLTW+ MLL+ +  WQ K+R EV   C    +   E  SKL L++ 
Sbjct: 389 CKTFFFAGHETTANLLTWSTMLLSLHQDWQEKLREEVFNECGKDKIPDAETCSKLKLMNT 448

Query: 392 VINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPE 451
           V  ESLRLY P   L R+A ED+KLG+LEIPKG +I +P+  +H  + +WG DA++FNP 
Sbjct: 449 VFMESLRLYGPVLNLLRLASEDMKLGNLEIPKGTTIILPIAKMHRDKAVWGSDADKFNPM 508

Query: 452 RFANSKAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSFTISDNYRHAPV 511
           RFAN  +  +      + F+ GPR C+GQ+FA+ME K +LAM++ +F   +S +Y+HAP 
Sbjct: 509 RFANGLSRAANHPNALLAFSMGPRACIGQNFAIMEAKTVLAMILQRFRLNLSADYKHAPA 568

Query: 512 VVLTIKPKYGVQVCLKPFN 520
             LT++P+Y + V L+P +
Sbjct: 569 DHLTLQPQYDLPVILEPID 571

BLAST of Sgr023949 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 362.5 bits (929), Expect = 5.8e-100
Identity = 192/493 (38.95%), Postives = 292/493 (59.23%), Query Frame = 0

Query: 35  PRRIKKTMAKQGVRGPKPRPLLGNILDVAALLSKSTSADMPAISHDIVPRLLPHFLSWSS 94
           P  + + +  QG+ GP  R   GN+ ++  +  +S  + +   S+DI PR+LPH+  W S
Sbjct: 31  PFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMS 90

Query: 95  QFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQQGSKHFIGRGLLMANGQN 154
           Q+G+ F++WNG EPR+C+++ +L K +LS       KS  + +  K    +GL+   G +
Sbjct: 91  QYGETFLYWNGTEPRICISDPELAKTMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGAD 150

Query: 155 WYHQRHIVAPAFMGDRLKSYAGYMVECTKEML-----QSIENEVNSGRSEFEIGEYMTRL 214
           W   R I+ PAF  DRLK     MV+CT +ML     +S + E    + + E+ E   RL
Sbjct: 151 WVRHRRILNPAFSIDRLKIMTTVMVDCTLKMLEEWRKESTKEETEHPKIKKEMNEEFQRL 210

Query: 215 TADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFFPSKYNREIKALKTK 274
           TADII+ + F SSY +G ++F     L+     +   + +PG+++ P+  N  +  L+ K
Sbjct: 211 TADIIATSAFGSSYVEGIEVFRSQMELKRCYTTSLNQVSIPGTQYLPTPSNIRVWKLERK 270

Query: 275 VEGLLMEIIQSRRDCVEVGRSSSYGNDLLGMLLNEMQKKKDGNGLSLNLQIIMDECKTFF 334
           ++  +  II SR     +   S YG+DLLG+LL       +G    ++++ I+ EC+TFF
Sbjct: 271 MDNSIKRIISSR-----LQSKSDYGDDLLGILLKAY--NTEGKERKMSIEEIIHECRTFF 330

Query: 335 FAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAVC-NGGTLSLEHLSKLSLLSMVINES 394
           F GHETT+ LL WT MLL+ +  WQ K+R E+   C    T   E  SKL L++MVI ES
Sbjct: 331 FGGHETTSNLLAWTTMLLSLHQDWQEKLREEIFKECGKEKTPDSETFSKLKLMNMVIMES 390

Query: 395 LRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSEELWGKDANEFNPERFANS 454
           LRLY P S L R A  +IKLGDLEIPKG ++ IP+L +H  + LWG DA++FNP RFAN 
Sbjct: 391 LRLYGPVSALAREASVNIKLGDLEIPKGTTVVIPLLKMHSDKTLWGSDADKFNPMRFANG 450

Query: 455 KAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKFSF-TISDNYRHAPVVVLT 514
            +  +      + F+ GPR C+GQ+F ++E K +L M++ +F F ++ D Y+H PV  +T
Sbjct: 451 VSRAANHPNALLAFSVGPRACIGQNFVMIEAKTVLTMILQRFRFISLCDEYKHTPVDNVT 510

Query: 515 IKPKYGVQVCLKP 518
           I+P+YG+ V L+P
Sbjct: 511 IQPQYGLPVMLQP 516

BLAST of Sgr023949 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 347.8 bits (891), Expect = 1.5e-95
Identity = 183/509 (35.95%), Postives = 293/509 (57.56%), Query Frame = 0

Query: 17  SVFIFSVAYDTISCYWLTPRRIKKTMAKQGVRGPKPRPLLGNILDVAALLSKSTSADMPA 76
           SV +  V    +S  W  PR+I++  +KQG+RGP     +GN+ ++  ++ K++S  MP 
Sbjct: 17  SVILSLVIVKGMSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPMP- 76

Query: 77  ISHDIVPRLLPHFLSWSSQFGKRFIFWNGIEPRMCLTETDLIKELLSKYSAVSGKSWLQQ 136
            SH+I+PR+L  +  W   +G  F+ W G   R+ + + DLI+E+ SK            
Sbjct: 77  FSHNILPRVLSFYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFYEKNE--AH 136

Query: 137 QGSKHFIGRGLLMANGQNWYHQRHIVAPAFMGDRLKSYAGYMVECTKEMLQSIENEVN-S 196
              K   G GLL   G+ W H R I++P F  + LK     +++   +M+    ++++ +
Sbjct: 137 PLVKQLEGDGLLSLKGEKWAHHRKIISPTFHMENLKLLVPVVLKSVTDMVDKWSDKLSEN 196

Query: 197 GRSEFEIGEYMTRLTADIISRTEFESSYEKGKQIFHLLTVLQHLCAQASRHLCLPGSRFF 256
           G  E ++ E+   LT D+ISRT F SSYE G+ +F L      LCA+A + + +PG RFF
Sbjct: 197 GEVEVDVYEWFQILTEDVISRTAFGSSYEDGRAVFRLQAQQMLLCAEAFQKVFIPGYRFF 256

Query: 257 PSKYNREIKALKTKVEGLLMEIIQSRRDCVEVGRSSS----YGNDLLGMLLNEMQKKKDG 316
           P++ N +   L  ++   L+++I+ RR     G           DLLG+++         
Sbjct: 257 PTRGNLKSWKLDKEIRKSLLKLIERRRQNAIDGEGEECKEPAAKDLLGLMI--------- 316

Query: 317 NGLSLNLQIIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQHKVRAEVMAVCNGGTL- 376
              ++ +Q I++ECK+FFFAG +TT+ LLTWT +LL+ +P WQ K R EV+ VC    + 
Sbjct: 317 QAKNVTVQDIVEECKSFFFAGKQTTSNLLTWTTILLSMHPEWQAKARDEVLRVCGSRDVP 376

Query: 377 SLEHLSKLSLLSMVINESLRLYPPASILPRMAFEDIKLGDLEIPKGLSIWIPVLAIHHSE 436
           + +H+ KL  LSM++NESLRLYPP     R A  D+KLG  +IP G  + IP++A+HH +
Sbjct: 377 TKDHVVKLKTLSMILNESLRLYPPIVATIRRAKSDVKLGGYKIPCGTELLIPIIAVHHDQ 436

Query: 437 ELWGKDANEFNPERFANSKAFTSGR---FIPFASGPRNCVGQSFALMETKIILAMLISKF 496
            +WG D NEFNP RFA+     +     FIPF  G R C+GQ+ A+++ K+ LA++I +F
Sbjct: 437 AIWGNDVNEFNPARFADGVPRAAKHPVGFIPFGLGVRTCIGQNLAILQAKLTLAVMIQRF 496

Query: 497 SFTISDNYRHAPVVVLTIKPKYGVQVCLK 517
           +F ++  Y+HAP V++ + P++G  +  +
Sbjct: 497 TFHLAPTYQHAPTVLMLLYPQHGAPITFR 513