Sgr023869 (gene) Monk fruit (Qingpiguo) v1

Overview


Name	Sgr023869
Type	gene
Organism	Siraitia grosvenorii (Monk fruit (Qingpiguo) v1)
Description	Cytoplasmic tRNA 2-thiolation protein 1
Location	tig00001047: 1034450 .. 1035007 (-)
RNA-Seq Expression	Sgr023869
Synteny	Sgr023869

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAAAGTCCAAATCCAGAAGACGAGTTGGACATCGCTGGTCCAAATATGAGCTTGGGCCACGTATGGGTTTGGGCCGGCCCAATGAGATATCTATGTTAATGGGCAAAGTCCATTTAAAAATTTCTTCAACAAAGTCTTAGCCGAAGTTGTTCATGAGCAAATGCATAGCATGAGAAGTTAGAACAAGCAATCCTTGTGACCAAGCTCCTCCCCATGCCTCCTTTGCCGCCCTCTCTTAACTTCTATAAGAACAGCTCCTCATCCTCGCCGCCGGCAAAGATCACATTAAGAAAAAGGAAAAAAAAAATGGAGAACCAAGAGAAGAAAGGAGGAGGAGGAGGGTTCTTTCAAATGCCTCTTCACTATCCCAGATACTCGAAGAAGGACTACGAGGACATGCCTGAGTGGAAGCTCGACCGCCTTCTCGCCGAGTACGGCCTCCCGACTCGAGGCGACTTGCCCTACAAGAGGCAGTTCGCCATGGGTGCTTTCCTCTGGCCTGATTTCCACCACAGCTTTAAAGCTCCTCTCTTCTCAACAAAAAAAGTTACATAG

mRNA sequence

ATGAAAAGTCCAAATCCAGAAGACGAGTTGGACATCGCTGGTCCAAATATGAGCTTGGGCCACCATGAGAAGTTAGAACAAGCAATCCTTGTGACCAAGCTCCTCCCCATGCCTCCTTTGCCGCCCTCTCTTAACTTCTATAAGAACAGCTCCTCATCCTCGCCGCCGGCAAAGATCACATTAAGAAAAAGGAAAAAAAAAATGGAGAACCAAGAGAAGAAAGGAGGAGGAGGAGGGTTCTTTCAAATGCCTCTTCACTATCCCAGATACTCGAAGAAGGACTACGAGGACATGCCTGAGTGGAAGCTCGACCGCCTTCTCGCCGAGTACGGCCTCCCGACTCGAGGCGACTTGCCCTACAAGAGGCAGTTCGCCATGGGTGCTTTCCTCTGGCCTGATTTCCACCACAGCTTTAAAGCTCCTCTCTTCTCAACAAAAAAAGTTACATAG

Coding sequence (CDS)

ATGAAAAGTCCAAATCCAGAAGACGAGTTGGACATCGCTGGTCCAAATATGAGCTTGGGCCACCATGAGAAGTTAGAACAAGCAATCCTTGTGACCAAGCTCCTCCCCATGCCTCCTTTGCCGCCCTCTCTTAACTTCTATAAGAACAGCTCCTCATCCTCGCCGCCGGCAAAGATCACATTAAGAAAAAGGAAAAAAAAAATGGAGAACCAAGAGAAGAAAGGAGGAGGAGGAGGGTTCTTTCAAATGCCTCTTCACTATCCCAGATACTCGAAGAAGGACTACGAGGACATGCCTGAGTGGAAGCTCGACCGCCTTCTCGCCGAGTACGGCCTCCCGACTCGAGGCGACTTGCCCTACAAGAGGCAGTTCGCCATGGGTGCTTTCCTCTGGCCTGATTTCCACCACAGCTTTAAAGCTCCTCTCTTCTCAACAAAAAAAGTTACATAG

Protein sequence

MKSPNPEDELDIAGPNMSLGHHEKLEQAILVTKLLPMPPLPPSLNFYKNSSSSSPPAKITLRKRKKKMENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMGAFLWPDFHHSFKAPLFSTKKVT

Homology

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BLAST of Sgr023869 vs. NCBI nr
Match: KAG6578420.1 (hypothetical protein SDJN03_22868, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015984.1 hypothetical protein SDJN02_21088, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 133.3 bits (334), Expect = 1.8e-27
Identity = 59/72 (81.94%), Postives = 63/72 (87.50%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           MEN EKK GG  FFQMPLHYPRYSK DYE+MPEWKLDRLL EYGLPT G+LPYKRQFAMG
Sbjct: 1   MENNEKKQGGDNFFQMPLHYPRYSKMDYENMPEWKLDRLLLEYGLPTHGNLPYKRQFAMG 60

Query: 128 AFLWPDFHHSFK 140
           AFLW D+H +FK
Sbjct: 61  AFLWHDYHPTFK 72

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BLAST of Sgr023869 vs. NCBI nr
Match: OVA17787.1 (hypothetical protein BVC80_1835g174 [Macleaya cordata])

HSP 1 Score: 119.8 bits (299), Expect = 2.0e-23
Identity = 51/66 (77.27%), Postives = 57/66 (86.36%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           MEN++K G    +FQMPLHYPRYS+KDYEDMPEWKLDRLL EYGLP  GDL YKR++AMG
Sbjct: 1   MENKKKNGVSSSYFQMPLHYPRYSRKDYEDMPEWKLDRLLMEYGLPAMGDLAYKREYAMG 60

Query: 128 AFLWPD 134
           AFLWPD
Sbjct: 61  AFLWPD 66

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BLAST of Sgr023869 vs. NCBI nr
Match: XP_010108400.1 (uncharacterized protein LOC21393700 [Morus notabilis] >EXC19391.1 hypothetical protein L484_010408 [Morus notabilis])

HSP 1 Score: 119.0 bits (297), Expect = 3.5e-23
Identity = 50/71 (70.42%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 71  QEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMGAFL 130
           ++K   G  +FQMPLHYPRY+K+DY+DMPEWKLD+LLA+YGLPT GDL YKR FA+GAFL
Sbjct: 5   KKKNESGVSYFQMPLHYPRYTKQDYQDMPEWKLDQLLAQYGLPTHGDLAYKRDFAIGAFL 64

Query: 131 WPDFHHSFKAP 142
           WPDFH   K P
Sbjct: 65  WPDFHQGSKPP 75

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BLAST of Sgr023869 vs. NCBI nr
Match: EOY18099.1 (Cytoplasmic tRNA 2-thiolation protein 1 [Theobroma cacao])

HSP 1 Score: 119.0 bits (297), Expect = 3.5e-23
Identity = 51/65 (78.46%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 71  QEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMGAFL 130
           + +K  G  FFQMPLHYPRY++KDY+DMPEWKLDRLLAEYGL  +GDL YKRQFAMGAFL
Sbjct: 2   ESEKQNGVSFFQMPLHYPRYTQKDYQDMPEWKLDRLLAEYGLSNKGDLAYKRQFAMGAFL 61

Query: 131 WPDFH 136
           WPDFH
Sbjct: 62  WPDFH 66

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BLAST of Sgr023869 vs. NCBI nr
Match: KAE8718042.1 (mannose-1-phosphate guanylyltransferase 1-like [Hibiscus syriacus])

HSP 1 Score: 118.6 bits (296), Expect = 4.5e-23
Identity = 52/68 (76.47%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           ME+ +++  G GFFQMPLHYPRY+ KDY DMPEWKLDRLLAEYGL   GDL YKR+FAMG
Sbjct: 1   MESGKQQVNGVGFFQMPLHYPRYTMKDYSDMPEWKLDRLLAEYGLSAEGDLDYKRRFAMG 60

Query: 128 AFLWPDFH 136
           AFLWPDFH
Sbjct: 61  AFLWPDFH 68

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BLAST of Sgr023869 vs. ExPASy TrEMBL
Match: A0A200R4Z5 (Uncharacterized protein OS=Macleaya cordata OX=56857 GN=BVC80_1835g174 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 9.8e-24
Identity = 51/66 (77.27%), Postives = 57/66 (86.36%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           MEN++K G    +FQMPLHYPRYS+KDYEDMPEWKLDRLL EYGLP  GDL YKR++AMG
Sbjct: 1   MENKKKNGVSSSYFQMPLHYPRYSRKDYEDMPEWKLDRLLMEYGLPAMGDLAYKREYAMG 60

Query: 128 AFLWPD 134
           AFLWPD
Sbjct: 61  AFLWPD 66

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BLAST of Sgr023869 vs. ExPASy TrEMBL
Match: A0A061FTQ6 (Cytoplasmic tRNA 2-thiolation protein 1 OS=Theobroma cacao OX=3641 GN=TCM_042740 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.7e-23
Identity = 51/65 (78.46%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 71  QEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMGAFL 130
           + +K  G  FFQMPLHYPRY++KDY+DMPEWKLDRLLAEYGL  +GDL YKRQFAMGAFL
Sbjct: 2   ESEKQNGVSFFQMPLHYPRYTQKDYQDMPEWKLDRLLAEYGLSNKGDLAYKRQFAMGAFL 61

Query: 131 WPDFH 136
           WPDFH
Sbjct: 62  WPDFH 66

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BLAST of Sgr023869 vs. ExPASy TrEMBL
Match: W9SNS4 (Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_010408 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.7e-23
Identity = 50/71 (70.42%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 71  QEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMGAFL 130
           ++K   G  +FQMPLHYPRY+K+DY+DMPEWKLD+LLA+YGLPT GDL YKR FA+GAFL
Sbjct: 5   KKKNESGVSYFQMPLHYPRYTKQDYQDMPEWKLDQLLAQYGLPTHGDLAYKRDFAIGAFL 64

Query: 131 WPDFHHSFKAP 142
           WPDFH   K P
Sbjct: 65  WPDFHQGSKPP 75

[Close]

BLAST of Sgr023869 vs. ExPASy TrEMBL
Match: A0A6A3BSS0 (Mannose-1-phosphate guanylyltransferase 1-like OS=Hibiscus syriacus OX=106335 GN=F3Y22_tig00110020pilonHSYRG00493 PE=4 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.2e-23
Identity = 52/68 (76.47%), Postives = 58/68 (85.29%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           ME+ +++  G GFFQMPLHYPRY+ KDY DMPEWKLDRLLAEYGL   GDL YKR+FAMG
Sbjct: 1   MESGKQQVNGVGFFQMPLHYPRYTMKDYSDMPEWKLDRLLAEYGLSAEGDLDYKRRFAMG 60

Query: 128 AFLWPDFH 136
           AFLWPDFH
Sbjct: 61  AFLWPDFH 68

[Close]

BLAST of Sgr023869 vs. ExPASy TrEMBL
Match: A0A6A1VXY6 (Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR3G014771 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.9e-23
Identity = 54/82 (65.85%), Postives = 62/82 (75.61%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           MEN+++ G     FQMPLHYPRY KK+Y DMPEWK+DRLLAEYGL   GDL YKR+FAMG
Sbjct: 1   MENRKRNGVSS--FQMPLHYPRYGKKEYLDMPEWKIDRLLAEYGLSVHGDLAYKREFAMG 60

Query: 128 AFLWPDFHHSFKAPLFSTKKVT 150
           AFLWPDFHH  K+  +   K T
Sbjct: 61  AFLWPDFHHDSKSTPYLNCKDT 80

[Close]

BLAST of Sgr023869 vs. TAIR 10
Match: AT3G55570.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41761.1); Has 128 Blast hits to 128 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 128; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 102.1 bits (253), Expect = 4.1e-22
Identity = 44/64 (68.75%), Postives = 53/64 (82.81%), Query Frame = 0


Query: 68  MENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMG 127
           M+ +  K G G  F+MPLHYPRYSK+DY+DMPEWKLDR+LA+YGL T GDL +KR FA+G
Sbjct: 19  MDVENGKNGEGSVFRMPLHYPRYSKEDYQDMPEWKLDRVLADYGLSTYGDLAHKRDFAIG 78

Query: 128 AFLW 132
           AFLW
Sbjct: 79  AFLW 82

[Close]

BLAST of Sgr023869 vs. TAIR 10
Match: AT5G41761.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55570.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 88.6 bits (218), Expect = 4.7e-18
Identity = 39/62 (62.90%), Postives = 46/62 (74.19%), Query Frame = 0


Query: 70  NQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGDLPYKRQFAMGAF 129
           N +K       FQ+PLHYP+Y+K DYE MPEW+LDRLL EYGLP  GD   KR+FA+GAF
Sbjct: 34  NHDKPQNQSSSFQIPLHYPKYTKSDYEKMPEWQLDRLLREYGLPVIGDSYEKRKFAIGAF 93

Query: 130 LW 132
           LW
Sbjct: 94  LW 95

[Close]

BLAST of Sgr023869 vs. TAIR 10
Match: AT3G09950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41761.1); Has 128 Blast hits to 128 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 128; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 76.3 bits (186), Expect = 2.4e-14
Identity = 40/79 (50.63%), Postives = 52/79 (65.82%), Query Frame = 0


Query: 63  KRKKKMENQEKKG---GGGGFFQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTRGD-- 122
           K ++ +EN +K G        F+MPLHYPRY+K+DYE+M EW+LD LL+EYGL    D  
Sbjct: 15  KNQESVENLDKNGAVKAPSSGFKMPLHYPRYTKEDYEEMEEWRLDLLLSEYGLLAFHDNT 74

Query: 123 LPYKRQFAMGAFLWPDFHH 137
           L  KR FA+  F+WP  HH
Sbjct: 75  LHEKRAFAIDTFIWP--HH 91

[Close]

BLAST of Sgr023869 vs. TAIR 10
Match: AT5G55620.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G09950.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 73.2 bits (178), Expect = 2.0e-13
Identity = 39/87 (44.83%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 47  YKNSSSSSPPAKITLRKRKKKMENQEKKGGGGGFFQMPLHYPRYSKKDYEDMPEWKLDRL 106
           Y N    +   + T  K  K M+ +E   G    FQ+PLHYP+YSK DYE M + +LD L
Sbjct: 17  YSNHMDQASNCQSTRNKIIKMMKKEEFPSG----FQVPLHYPKYSKSDYEVMDDLRLDLL 76

Query: 107 LAEYGLPTRGDLPYKRQFAMGAFLWPD 134
           L +YG    G L  KR FA+ +FLWPD
Sbjct: 77  LKQYGFSFEGSLEDKRVFAIESFLWPD 99

[Close]

BLAST of Sgr023869 vs. TAIR 10
Match: AT3G11405.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G55570.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 67.8 bits (164), Expect = 8.5e-12
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 0


Query: 81  FQMPLHYPRYSKKDYEDMPEWKLDRLLAEYGLPTR-GDLPYKRQFAMGAFLW 132
           FQMPL YP Y+K+ Y+ M E +LDRLL  YGLPT  G+L  K++FA+GAFLW
Sbjct: 57  FQMPLQYPNYAKEQYDIMSEEELDRLLKLYGLPTDIGNLSCKKEFAVGAFLW 108

The following BLAST results are available for this feature:

BLAST of Sgr023869 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6578420.1	1.8e-27	81.94	hypothetical protein SDJN03_22868, partial [Cucurbita argyrosperma subsp. sorori...	[more]
OVA17787.1	2.0e-23	77.27	hypothetical protein BVC80_1835g174 [Macleaya cordata]	[more]
XP_010108400.1	3.5e-23	70.42	uncharacterized protein LOC21393700 [Morus notabilis] >EXC19391.1 hypothetical p...	[more]
EOY18099.1	3.5e-23	78.46	Cytoplasmic tRNA 2-thiolation protein 1 [Theobroma cacao]	[more]
KAE8718042.1	4.5e-23	76.47	mannose-1-phosphate guanylyltransferase 1-like [Hibiscus syriacus]	[more]

BLAST of Sgr023869 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Sgr023869 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A200R4Z5	9.8e-24	77.27	Uncharacterized protein OS=Macleaya cordata OX=56857 GN=BVC80_1835g174 PE=4 SV=1	[more]
A0A061FTQ6	1.7e-23	78.46	Cytoplasmic tRNA 2-thiolation protein 1 OS=Theobroma cacao OX=3641 GN=TCM_042740...	[more]
W9SNS4	1.7e-23	70.42	Uncharacterized protein OS=Morus notabilis OX=981085 GN=L484_010408 PE=4 SV=1	[more]
A0A6A3BSS0	2.2e-23	76.47	Mannose-1-phosphate guanylyltransferase 1-like OS=Hibiscus syriacus OX=106335 GN...	[more]
A0A6A1VXY6	4.9e-23	65.85	Uncharacterized protein OS=Morella rubra OX=262757 GN=CJ030_MR3G014771 PE=4 SV=1	[more]

BLAST of Sgr023869 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55570.1	4.1e-22	68.75	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G41761.1	4.7e-18	62.90	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G09950.1	2.4e-14	50.63	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G55620.1	2.0e-13	44.83	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT3G11405.1	8.5e-12	61.54	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 45..81
None	No IPR available	PANTHER	PTHR33513:SF24	CYTOPLASMIC TRNA 2-THIOLATION PROTEIN-RELATED	coord: 62..137
None	No IPR available	PANTHER	PTHR33513	OS06G0523300 PROTEIN	coord: 62..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Sgr023869.1	Sgr023869.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016779	nucleotidyltransferase activity