Sgr023736 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023736 vs. NCBI nr
Match: XP_022140673.1 (aminoacylase-1 [Momordica charantia])

HSP 1 Score: 831.6 bits (2147), Expect = 3.7e-237
Identity = 407/448 (90.85%), Postives = 425/448 (94.87%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MN   +NLLLS IT+FLFLSSS A E+ SSSSAII+RFQQYLQI+TVQPNP YYEAADFI
Sbjct: 1   MNLKYVNLLLSVITIFLFLSSSIADEESSSSSAIIARFQQYLQINTVQPNPHYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSL+LES TIEFVEGKPLILLKWPGSNP L SILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLALESQTIEFVEGKPLILLKWPGSNPDLSSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDSLGNIY RGSQDMKCVGMQYLEAIRRLKA GFEP R+VYLSFVPDEEIGG  GAE+F
Sbjct: 121 HIDSLGNIYARGSQDMKCVGMQYLEAIRRLKALGFEPARSVYLSFVPDEEIGGRAGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEFQKLNVGIVLDEGLPSP E+YR FYGERSPWW+VIKAVGAPGHGAKLYDNTALEN
Sbjct: 181 AESDEFQKLNVGIVLDEGLPSPGENYRVFYGERSPWWMVIKAVGAPGHGAKLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIESVRRFRASQFDL+KAGLKAEGEVVSVNMVFLK+GTPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESVRRFRASQFDLVKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKES+F+KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESIFDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIE YESIIKAYAS+I   KPTESF+DE
Sbjct: 421 GIEAYESIIKAYASFI-AHKPTESFKDE 447

BLAST of Sgr023736 vs. NCBI nr
Match: XP_038876663.1 (aminoacylase-1 [Benincasa hispida])

HSP 1 Score: 821.2 bits (2120), Expect = 4.9e-234
Identity = 401/448 (89.51%), Postives = 421/448 (93.97%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           M F S++ LLSFITLFL LS S + EDPSS+SAIISRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MIFKSLHFLLSFITLFLLLSVSTSEEDPSSTSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAK+LSLES TIEFVEGKPLILLKWPG NP LPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKALSLESQTIEFVEGKPLILLKWPGLNPDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGFEP+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFEPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESD F KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGAKLYDN ALEN
Sbjct: 181 AESDVFNKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGAKLYDNAALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIES+RRFRASQFDLIKAGLK EG+VVSVNMVFLK+GTPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESIRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPTANPESLERRIAEEWAP SRNMTFEFKQKESV+NKFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPASRNMTFEFKQKESVYNKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVY+SIIKAYASY  G KPTESF+DE
Sbjct: 421 GIEVYKSIIKAYASY-DGDKPTESFKDE 447

BLAST of Sgr023736 vs. NCBI nr
Match: KAA0047810.1 (aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 814.3 bits (2102), Expect = 6.0e-232
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + +DPSS SAI+SRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPLI+LKWPGSN  LPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+++KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G K  ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKRMESFKDE 447

BLAST of Sgr023736 vs. NCBI nr
Match: XP_004139978.1 (aminoacylase-1 [Cucumis sativus] >KGN46643.1 hypothetical protein Csa_005514 [Cucumis sativus])

HSP 1 Score: 813.5 bits (2100), Expect = 1.0e-231
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + +DPSS SAIISRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPL++LKWPGSNP LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVR+PPT NPESLERRIAEEWAP SRNMTFEFKQKES++NKFGKPALTA DKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G KP ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKPMESFKDE 447

BLAST of Sgr023736 vs. NCBI nr
Match: XP_008448158.1 (PREDICTED: aminoacylase-1 [Cucumis melo] >TYK01049.1 aminoacylase-1 [Cucumis melo var. makuwa])

HSP 1 Score: 812.8 bits (2098), Expect = 1.8e-231
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + ++PSS SAIISRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPLI+LKWPGSN  LPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+++KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G K  ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKRMESFKDE 447

BLAST of Sgr023736 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 1.4e-103
Identity = 190/411 (46.23%), Postives = 256/411 (62.29%), Query Frame = 0

Query: 76  EDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLSLESHTIEFVEGKPLILL 135
           +DP S    ++ F+QYL+I TVQPNP Y  A  F+  +A+ L L    IE V G  + +L
Sbjct: 4   KDPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVL 63

Query: 136 KWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNIYGRGSQDMKCVGMQYLE 195
            WPG+NPSLPSILLNSHTDVVP     W H P  A  DS G IY RGSQDMK V +QYLE
Sbjct: 64  TWPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLE 123

Query: 196 AIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQKLNVGIVLDEGLPSPDEH 255
           A+RRLK+ G    RT++++FVPDEE+GGH G E F +  EFQ L  G  LDEGL +P + 
Sbjct: 124 AVRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDA 183

Query: 256 YRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLIKAG--L 315
           +  FY ERSPWW+ + + G PGH ++  ++TA E L K I S+  FR  +   ++A   L
Sbjct: 184 FTVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHL 243

Query: 316 KAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAP 375
           K EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E+++      
Sbjct: 244 K-EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQE 303

Query: 376 VSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHANGKLGKPEIFPASTDA 435
               +TFEF Q      KF +P +T TD S+PWW     A +  N  L +PEIFPA+TD+
Sbjct: 304 AGEGVTFEFAQ------KFTEPRMTPTDDSDPWWAAFSGACKAMNLTL-EPEIFPAATDS 363

Query: 436 RYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 485
           RY R +G+PA+GFSPM  TP+LLHDHNE L++  +L+G+++Y  ++ A AS
Sbjct: 364 RYIRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

BLAST of Sgr023736 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 8.8e-101
Identity = 184/409 (44.99%), Postives = 252/409 (61.61%), Query Frame = 0

Query: 78  PSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLSLESHTIEFVEGKPLILLKW 137
           P S    ++ F+QYL+I TVQPNP Y  A  F+  +A+ L L    IE   G  + +L W
Sbjct: 6   PESEHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTW 65

Query: 138 PGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNIYGRGSQDMKCVGMQYLEAI 197
           PG+NP L SILLNSHTDVVP     W H P  A  DS G IY RG+QDMK V +QYLEA+
Sbjct: 66  PGTNPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAV 125

Query: 198 RRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQKLNVGIVLDEGLPSPDEHYR 257
           RRLK+ G    RT++++FVPDEE+GGH G E F +  EFQ L  G  LDEGL +P + + 
Sbjct: 126 RRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFT 185

Query: 258 AFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLIKAG--LKA 317
            FY ERSPWW+ + + G PGH ++  ++TA E L K + S+  FR  +   ++A   LK 
Sbjct: 186 VFYSERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLK- 245

Query: 318 EGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPVS 377
           EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E+++        
Sbjct: 246 EGAVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAG 305

Query: 378 RNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHANGKLGKPEIFPASTDARY 437
             +TFEF Q      KF +P +T TD ++PWW     A +  N  L +PEIFPA+TD+RY
Sbjct: 306 EGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRY 365

Query: 438 FRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 485
            R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 366 IRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of Sgr023736 vs. ExPASy Swiss-Prot
Match: Q6PTT0 (Aminoacylase-1B OS=Rattus norvegicus OX=10116 GN=Acy1b PE=1 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.1e-98
Identity = 178/412 (43.20%), Postives = 250/412 (60.68%), Query Frame = 0

Query: 75  AEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLSLESHTIEFVEGKPLIL 134
           ++ P      ++ F+QYL+I TVQP P Y  A  F    A+ L L    +E   G  + +
Sbjct: 3   SKGPEEEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTV 62

Query: 135 LKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNIYGRGSQDMKCVGMQYL 194
           L WPG+NP+L SILLNSHTDVVP     W+H P  A  DS G IY RG+QDMKCV +QYL
Sbjct: 63  LTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYTRGAQDMKCVSIQYL 122

Query: 195 EAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQKLNVGIVLDEGLPSPDE 254
           EA++RLK  G    RT++++FVPDEE+GGH G E F +  EF  L  G  LDEGL +P +
Sbjct: 123 EAVKRLKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRHEFHALRAGFALDEGLANPTD 182

Query: 255 HYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLIKAG-- 314
            +  FY ERSPWW+ + + G PGH ++  ++TA E L K + S+  FR  ++  +++   
Sbjct: 183 AFTVFYSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPH 242

Query: 315 LKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWA 374
           LK EG V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E ++     
Sbjct: 243 LK-EGSVTSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEEQLQSWCQ 302

Query: 375 PVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHANGKLGKPEIFPASTD 434
                +TFEF Q      KF +P +T TD ++PWW     A +     L +PEIFPA+TD
Sbjct: 303 EAGEGVTFEFAQ------KFTEPRMTPTDDTDPWWAAFSGACKEMTLTL-EPEIFPAATD 362

Query: 435 ARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYAS 485
           +RY R +G+PA+GFSPM  TP+LLHDHNE L++A +L+G+++Y  ++ A AS
Sbjct: 363 SRYIRAVGIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALAS 400

BLAST of Sgr023736 vs. ExPASy Swiss-Prot
Match: P37111 (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 360.9 bits (925), Expect = 2.4e-98
Identity = 185/425 (43.53%), Postives = 254/425 (59.76%), Query Frame = 0

Query: 70  SSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLSLESHTIEFVEG 129
           S     E PS     ++ F+QYL+I TVQP P Y  A  F+  +A+ L L    +E V G
Sbjct: 3   SKGREGEHPS-----VTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPG 62

Query: 130 KPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNIYGRGSQDMKCV 189
             + +L WPG+NP+L SILLNSHTDVVP     W+H P     D+ G IYGRG+QDMKCV
Sbjct: 63  HVVTVLTWPGTNPTLSSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCV 122

Query: 190 GMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQKLNVGIVLDEGL 249
            +QYLEA+RRLK  G    RT++++FVPDEE+GGH G E F +  EFQ L  G  LDEGL
Sbjct: 123 SIQYLEAVRRLKVEGHHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGL 182

Query: 250 PSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLI 309
            SP + +  FY ERSPWWL + + G PGHG++  ++TA E L K I S+  FR  +   +
Sbjct: 183 ASPTDAFTVFYSERSPWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRL 242

Query: 310 KAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAE 369
           ++     G V SVN+  L+ G         N+ P+   A FD R+ P  + ++ E ++  
Sbjct: 243 QSNQLKPGAVTSVNLTMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQS 302

Query: 370 EWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHANGKLGKPEIFPA 429
                   +TFEF Q      K+ +  +T+TD S+PWW       +     L + EI PA
Sbjct: 303 WCQAAGEGVTFEFVQ------KWMETQVTSTDDSDPWWAAFSGVFKDMKLAL-ELEICPA 362

Query: 430 STDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESIIKAYASYIVGQ 489
           STDARY R  G+PA+GFSPM +TP+LLHDH+E L++A +L+G+++Y  ++ A AS  V  
Sbjct: 363 STDARYIRAAGVPALGFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALAS--VPA 407

Query: 490 KPTES 495
            P+ES
Sbjct: 423 LPSES 407

BLAST of Sgr023736 vs. ExPASy Swiss-Prot
Match: Q55DP8 (Aminoacylase-1 OS=Dictyostelium discoideum OX=44689 GN=acy1 PE=2 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 2.0e-97
Identity = 181/402 (45.02%), Postives = 257/402 (63.93%), Query Frame = 0

Query: 85  ISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSL 144
           ++ F+++L+I T  P P Y  +  F+V +AK  ++  + +    G P++L+K  G  P+L
Sbjct: 10  VTVFREFLKIRTDHPTPDYESSTKFLVEKAKEYNI-PYEVYRETGTPIVLMKIEGLEPNL 69

Query: 145 PSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASG 204
            ++LLNSH DVVPA H  W   P  A  D  GNI+GRG+QDMKCV MQ+LE  RR+  SG
Sbjct: 70  KTVLLNSHVDVVPAVHDSWKVDPFSAWKDESGNIFGRGTQDMKCVCMQFLEVARRIVQSG 129

Query: 205 FEPVRTVYLSFVPDEEIGGHG-GAERFAESDEFQKLNVGIVLDEGLPSPDEHYRAFYGER 264
            +  RT++LSFVPDEEIGG G G E+F  +++F++LN+G+ LDEGL SP   +  FYGER
Sbjct: 130 QKLKRTLHLSFVPDEEIGGSGKGMEKFVYTEKFRQLNIGLCLDEGLASPTNDFTVFYGER 189

Query: 265 SPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESVRRFRASQFDLIKAGL----KAEGEV 324
           +PWW+ I AVG  GHG++  + TA+E L ++I  +  FR  QF+ +  G     K  G+V
Sbjct: 190 APWWVHITAVGNAGHGSRFIEGTAIEKLMRTINKMLAFRQEQFESLHHGQHECGKKLGDV 249

Query: 325 VSVNMVFLKAGTP--SPTGFVMNLQPSEAEAGFDVRLPPTANPESLERRIAEEWAPVSRN 384
            S+N+  LKAG P      F  N+ P++AEAGFD+R+PPT N +    +I +EW      
Sbjct: 250 TSLNLTVLKAGIPIDHSNNFSYNVIPTQAEAGFDIRIPPTVNLDQFLDQI-KEWT-AEEG 309

Query: 385 MTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHANGKLGKPEIFPASTDARYFR 444
           ++F+F       +   K  +T  D  N WW   +E+ +  +  L   EIFPA+TD+R+ R
Sbjct: 310 LSFKFA------SYIPKNEMTKLDSDNKWWENFKESCKKMDINL-VTEIFPAATDSRFIR 369

Query: 445 NLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESII 480
           NLG+PA GFSP+ NTPILLHDHNEFLN+  YL+GI+++  II
Sbjct: 370 NLGIPAFGFSPINNTPILLHDHNEFLNEKVYLRGIDIFMGII 401

BLAST of Sgr023736 vs. ExPASy TrEMBL
Match: A0A6J1CII7 (N-acyl-L-amino-acid amidohydrolase OS=Momordica charantia OX=3673 GN=LOC111011275 PE=3 SV=1)

HSP 1 Score: 831.6 bits (2147), Expect = 1.8e-237
Identity = 407/448 (90.85%), Postives = 425/448 (94.87%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MN   +NLLLS IT+FLFLSSS A E+ SSSSAII+RFQQYLQI+TVQPNP YYEAADFI
Sbjct: 1   MNLKYVNLLLSVITIFLFLSSSIADEESSSSSAIIARFQQYLQINTVQPNPHYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSL+LES TIEFVEGKPLILLKWPGSNP L SILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLALESQTIEFVEGKPLILLKWPGSNPDLSSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDSLGNIY RGSQDMKCVGMQYLEAIRRLKA GFEP R+VYLSFVPDEEIGG  GAE+F
Sbjct: 121 HIDSLGNIYARGSQDMKCVGMQYLEAIRRLKALGFEPARSVYLSFVPDEEIGGRAGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEFQKLNVGIVLDEGLPSP E+YR FYGERSPWW+VIKAVGAPGHGAKLYDNTALEN
Sbjct: 181 AESDEFQKLNVGIVLDEGLPSPGENYRVFYGERSPWWMVIKAVGAPGHGAKLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIESVRRFRASQFDL+KAGLKAEGEVVSVNMVFLK+GTPSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIESVRRFRASQFDLVKAGLKAEGEVVSVNMVFLKSGTPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKES+F+KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESIFDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIE YESIIKAYAS+I   KPTESF+DE
Sbjct: 421 GIEAYESIIKAYASFI-AHKPTESFKDE 447

BLAST of Sgr023736 vs. ExPASy TrEMBL
Match: A0A5A7TXA6 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G00610 PE=3 SV=1)

HSP 1 Score: 814.3 bits (2102), Expect = 2.9e-232
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + +DPSS SAI+SRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIVSRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPLI+LKWPGSN  LPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+++KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G K  ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKRMESFKDE 447

BLAST of Sgr023736 vs. ExPASy TrEMBL
Match: A0A0A0KAV8 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis sativus OX=3659 GN=Csa_6G117740 PE=3 SV=1)

HSP 1 Score: 813.5 bits (2100), Expect = 5.0e-232
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + +DPSS SAIISRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQDPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPL++LKWPGSNP LPSILLNSHTDVVPAEH KWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLVILKWPGSNPELPSILLNSHTDVVPAEHKKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVR+PPT NPESLERRIAEEWAP SRNMTFEFKQKES++NKFGKPALTA DKSNP WNLL
Sbjct: 301 DVRVPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYNKFGKPALTAIDKSNPRWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G KP ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKPMESFKDE 447

BLAST of Sgr023736 vs. ExPASy TrEMBL
Match: A0A5D3BSI0 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold264G00590 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 8.5e-232
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + ++PSS SAIISRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPLI+LKWPGSN  LPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+++KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G K  ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKRMESFKDE 447

BLAST of Sgr023736 vs. ExPASy TrEMBL
Match: A0A1S3BJ13 (N-acyl-L-amino-acid amidohydrolase OS=Cucumis melo OX=3656 GN=LOC103490443 PE=3 SV=1)

HSP 1 Score: 812.8 bits (2098), Expect = 8.5e-232
Identity = 395/448 (88.17%), Postives = 420/448 (93.75%), Query Frame = 0

Query: 51  MNFNSINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFI 110
           MNF S++ LLS ITLF FLS S + ++PSS SAIISRFQQYLQI+TVQP+PQYYEAADFI
Sbjct: 1   MNFKSLHFLLSIITLFFFLSISTSEQNPSSPSAIISRFQQYLQINTVQPSPQYYEAADFI 60

Query: 111 VSQAKSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGA 170
           +SQAKSLSLESHTIEFVEGKPLI+LKWPGSN  LPSILLNSHTDVVPAEHHKWTHPPLGA
Sbjct: 61  ISQAKSLSLESHTIEFVEGKPLIILKWPGSNLDLPSILLNSHTDVVPAEHHKWTHPPLGA 120

Query: 171 HIDSLGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERF 230
           HIDS GNIY RGSQDMKCVGMQYLEAIRRLKASGF+P+R+VYLSFVPDEEIGGHGGAE+F
Sbjct: 121 HIDSHGNIYARGSQDMKCVGMQYLEAIRRLKASGFQPLRSVYLSFVPDEEIGGHGGAEKF 180

Query: 231 AESDEFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALEN 290
           AESDEF+KLNV IVLDEGLPSP E+YR FYGE+SPWWLVIKAVGAPGHGA+LYDNTALEN
Sbjct: 181 AESDEFKKLNVAIVLDEGLPSPGENYRVFYGEKSPWWLVIKAVGAPGHGARLYDNTALEN 240

Query: 291 LFKSIESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGF 350
           LFKSIE+VRRFRASQFDLIKAGLK EG+VVSVNMVFLK+G PSPTGFVMNLQPSEAEAGF
Sbjct: 241 LFKSIETVRRFRASQFDLIKAGLKTEGDVVSVNMVFLKSGIPSPTGFVMNLQPSEAEAGF 300

Query: 351 DVRLPPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLL 410
           DVRLPPT NPESLERRIAEEWAP SRNMTFEFKQKES+++KFGKPALTA DKSNPWWNLL
Sbjct: 301 DVRLPPTTNPESLERRIAEEWAPASRNMTFEFKQKESIYDKFGKPALTAIDKSNPWWNLL 360

Query: 411 EEAVRHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLK 470
           EEAVR+ANGKL  PEIFPASTDARYFRNLGLPAIGFSPM NTPILLHDHNEFLNQAEYLK
Sbjct: 361 EEAVRNANGKLANPEIFPASTDARYFRNLGLPAIGFSPMVNTPILLHDHNEFLNQAEYLK 420

Query: 471 GIEVYESIIKAYASYIVGQKPTESFRDE 499
           GIEVYESIIKAYASY  G K  ESF+DE
Sbjct: 421 GIEVYESIIKAYASY-DGDKRMESFKDE 447

BLAST of Sgr023736 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 647.5 bits (1669), Expect = 9.1e-186
Identity = 314/427 (73.54%), Postives = 360/427 (84.31%), Query Frame = 0

Query: 59  LLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLS 118
           LL  + + + L  SA A D     AI+SRFQ+YL+I+TVQPNP+YY+A DFI+SQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 119 LESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNI 178
           LES TIEFV+GKPL+LLKW GS+P+LP+ LLNSHTDVVP E  KWTH PL AH+D  G+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 179 YGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQK 238
           Y RGSQDMKCVGMQYLEAIR+L+ASGF+P+R+VYLSFVPDEEIGGH GAE+FAES  F+ 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 239 LNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESV 298
           LN+ IVLDEGLPSP E YR FYGERSPWWLVIKA G PGHGAKLYDN+A+ENL KSIES+
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 299 RRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTA 358
           RRFRASQFDL+KAG  AEG+VVSVNM FLKAGTPSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 359 NPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHAN 418
           + E+LERR+ EEWAP +RNM+FEFKQK +     GK  LTA D SNPWW LLE AV+ A 
Sbjct: 303 DAEALERRLVEEWAPAARNMSFEFKQKLT-----GKQFLTAADDSNPWWGLLENAVKEAG 362

Query: 419 GKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESI 478
           G+  KPEIFPASTDARYFR  G+PA GFSP++NTP LLHDHNE+L +AEYLKGIEVY SI
Sbjct: 363 GRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSI 420

Query: 479 IKAYASY 486
           IKAYASY
Sbjct: 423 IKAYASY 420

BLAST of Sgr023736 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 642.1 bits (1655), Expect = 3.8e-184
Identity = 314/430 (73.02%), Postives = 360/430 (83.72%), Query Frame = 0

Query: 59  LLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLS 118
           LL  + + + L  SA A D     AI+SRFQ+YL+I+TVQPNP+YY+A DFI+SQAK LS
Sbjct: 3   LLRLLLVVVVLHLSAVAGD----DAIVSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLS 62

Query: 119 LESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNI 178
           LES TIEFV+GKPL+LLKW GS+P+LP+ LLNSHTDVVP E  KWTH PL AH+D  G+I
Sbjct: 63  LESQTIEFVKGKPLLLLKWVGSDPTLPAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDI 122

Query: 179 YGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQK 238
           Y RGSQDMKCVGMQYLEAIR+L+ASGF+P+R+VYLSFVPDEEIGGH GAE+FAES  F+ 
Sbjct: 123 YARGSQDMKCVGMQYLEAIRKLQASGFKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKS 182

Query: 239 LNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESV 298
           LN+ IVLDEGLPSP E YR FYGERSPWWLVIKA G PGHGAKLYDN+A+ENL KSIES+
Sbjct: 183 LNIAIVLDEGLPSPTESYRVFYGERSPWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESI 242

Query: 299 RRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTA 358
           RRFRASQFDL+KAG  AEG+VVSVNM FLKAGTPSPTGFVMNLQPSEAEAGFD+R+PP+ 
Sbjct: 243 RRFRASQFDLLKAGGIAEGDVVSVNMAFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSV 302

Query: 359 NPESLERRIAEEWAPVSRNMTFE---FKQKESVFNKFGKPALTATDKSNPWWNLLEEAVR 418
           + E+LERR+ EEWAP +RNM+FE   FKQK +     GK  LTA D SNPWW LLE AV+
Sbjct: 303 DAEALERRLVEEWAPAARNMSFELGQFKQKLT-----GKQFLTAADDSNPWWGLLENAVK 362

Query: 419 HANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVY 478
            A G+  KPEIFPASTDARYFR  G+PA GFSP++NTP LLHDHNE+L +AEYLKGIEVY
Sbjct: 363 EAGGRTSKPEIFPASTDARYFRKAGVPAFGFSPISNTPSLLHDHNEYLGKAEYLKGIEVY 422

Query: 479 ESIIKAYASY 486
            SIIKAYASY
Sbjct: 423 VSIIKAYASY 423

BLAST of Sgr023736 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 523.9 bits (1348), Expect = 1.5e-148
Identity = 243/431 (56.38%), Postives = 316/431 (73.32%), Query Frame = 0

Query: 55  SINLLLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQA 114
           +++LLL  + LF F+ S  +          I+RFQQYL+ +T  PNP Y     F++ QA
Sbjct: 2   AVSLLLWTLFLFSFIFSLQSHSKEEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQA 61

Query: 115 KSLSLESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDS 174
           +S+ L S TIEFV GKP++LL W GSN +L SIL NSH D VPAE  KW HPP  AH   
Sbjct: 62  RSIGLTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTI 121

Query: 175 LGNIYGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESD 234
            G+IY RG+QD KC+G+QYLEAIR LK+  F P+RT+++S+VP+EEIGG GG  +FA S 
Sbjct: 122 DGHIYARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASS 181

Query: 235 EFQKLNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKS 294
           EF+ LN+G V+DEG  SP + +R FY ER+PW L I+A G PGHGAKLYDN+A+ENL KS
Sbjct: 182 EFKDLNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKS 241

Query: 295 IESVRRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRL 354
           +E + RFR SQFD +KAG  A  EV+SVN V+LKAGTPS TGFVMN+QPSEAE G+D+RL
Sbjct: 242 VELISRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRL 301

Query: 355 PPTANPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAV 414
           PP A+P +L++RIAEEWAP  RNMT+  KQ+  + +  G+P +T T+ +NPWW++ ++AV
Sbjct: 302 PPMADPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAV 361

Query: 415 RHANGKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEV 474
               GKL KPEI  ++TD+R+ R LG+P +GFSPM NTPIL+HDHNEFL    ++KGI V
Sbjct: 362 EATGGKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGV 421

Query: 475 YESIIKAYASY 486
           YES+I A +S+
Sbjct: 422 YESVISALSSF 432

BLAST of Sgr023736 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 523.5 bits (1347), Expect = 2.0e-148
Identity = 239/427 (55.97%), Postives = 316/427 (74.00%), Query Frame = 0

Query: 59  LLSFITLFLFLSSSAAAEDPSSSSAIISRFQQYLQIDTVQPNPQYYEAADFIVSQAKSLS 118
           L+ F  +F   SSS+  + P      I+RFQQYL+ +T  PNP Y     F+++QA+S+ 
Sbjct: 10  LIIFSIIFSLQSSSSEEDTP------ITRFQQYLRFNTAHPNPNYTAPISFLINQAQSIG 69

Query: 119 LESHTIEFVEGKPLILLKWPGSNPSLPSILLNSHTDVVPAEHHKWTHPPLGAHIDSLGNI 178
           L + TIEF+ GKP++L+ W GSNP+LPSIL NSH D VPAE  KWT+PP  AH    G+I
Sbjct: 70  LTTKTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHKTIDGHI 129

Query: 179 YGRGSQDMKCVGMQYLEAIRRLKASGFEPVRTVYLSFVPDEEIGGHGGAERFAESDEFQK 238
           Y RG+QD KC+G+QYLE+IR LK+ GF P+RT+++S+VP+EEIGG  G  +FA S EF+ 
Sbjct: 130 YARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAASSEFKD 189

Query: 239 LNVGIVLDEGLPSPDEHYRAFYGERSPWWLVIKAVGAPGHGAKLYDNTALENLFKSIESV 298
           LN+G  +DEG  +P + +R FY +R PW  VIKA G PGHGAKLYDN+A+ENL KS+E +
Sbjct: 190 LNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLMKSVELI 249

Query: 299 RRFRASQFDLIKAGLKAEGEVVSVNMVFLKAGTPSPTGFVMNLQPSEAEAGFDVRLPPTA 358
            RFR SQFD +KAG  A  EV+SVN V+LKAGTP+ TGFVMN+QPSEAEAG+D+RLPP A
Sbjct: 250 SRFRESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDLRLPPMA 309

Query: 359 NPESLERRIAEEWAPVSRNMTFEFKQKESVFNKFGKPALTATDKSNPWWNLLEEAVRHAN 418
           +P+ +++RIAEEWAP  RNMT+  ++K  + +  G+P +T  + SNPWW++ ++AV    
Sbjct: 310 DPDVMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQAVEAMG 369

Query: 419 GKLGKPEIFPASTDARYFRNLGLPAIGFSPMANTPILLHDHNEFLNQAEYLKGIEVYESI 478
           GKL KPEI  ++TD+R+ R LG+P  GFSPM NTPILLHDHNEFL    ++KGIEVYES+
Sbjct: 370 GKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGIEVYESV 429

Query: 479 IKAYASY 486
           I A +S+
Sbjct: 430 ISALSSF 430