Sgr023648 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023648 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 585.9 bits (1509), Expect = 2.8e-163
Identity = 289/401 (72.07%), Postives = 326/401 (81.30%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPV 68
           LDEKDKQALQ +EDV R ADEIQRRVLTEILSTNAHVEYLQ++GL+GSTDASTFK LIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  VSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNY 128
           VSYE LKPYIDRI NGDDSPILCA+PITEF+ S+GTSGGE KLIP  E+EFAR+ SI NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMP-SGSYTSP 188
           + P MKQLFPGL  GK M+F F + ES TKAGIVAR + TS  K  H  N+    SYTSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 248
            DI+ CTD+YQSLY Q LCGLCQN KV+RVG++FASTLI++F FLE HWVLLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 INPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSMS 308
           IN KI D  VR+SVMNILKP+P+LAD IE ECSKG WEGI+TRLWPNTK+I+A+VTGSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRGN 368
            YIP L+YY   LPLI   Y SSE FFG+NL+P  KPEEISYTFIPT+AYFEFLP+DR N
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 GFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
                       + EV +E VDLVDVKLGQEYE+VVTTYAG
Sbjct: 364 D-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAG 393

BLAST of Sgr023648 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 569.3 bits (1466), Expect = 2.7e-158
Identity = 288/412 (69.90%), Postives = 337/412 (81.80%), Query Frame = 0

Query: 1   MSNGGENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDAS 60
           MS+  E+ LDEKDK+ALQHIEDV   ADEIQR++LTEILSTNA+VEYLQ++GL GSTDAS
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFKKLIPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFA 120
           TFKKLIP++SYEQL PY+  IA+ DDSPILC+NPIT FF SSGTSGGE KLIP++EEEF 
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 RRSSILNYVMPMMKQLFPGLD--KGKGMYFGFTRAESWTKAGIVARPMFTSLCKR-SHFK 180
           RR S  NY+MP MKQLFP ++  KGKGM F F + E  TKAGI+ R +FTSL KR S+ K
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNLK 180

Query: 181 NMPSGSYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWV 240
           + P G+ TSP+DIILCTD +QSLY Q LCGL QND V R+GA+FASTLIHVF FLE HWV
Sbjct: 181 SKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHWV 240

Query: 241 LLANDIRTGTINPKISDPSVRESVMNI-LKPNPKLADLIEYECSKGMWEGIITRLWPNTK 300
           +LANDI TG+INPKI++ SVRESVM I +KPNP++ADLIE+ECSKG WEGII RLWPNTK
Sbjct: 241 VLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300

Query: 301 YIDAIVTGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMA 360
           YI+AIVTGSMSQYIP+L++Y+N LP++ + YGSSE F GLNL+P   P EISYT IPTMA
Sbjct: 301 YINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 360

Query: 361 YFEFLPIDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           YFEFLPIDR N            N EV ++LVDLVDVKLGQEYELV+TT+AG
Sbjct: 361 YFEFLPIDRTN-----------PNGEVTQQLVDLVDVKLGQEYELVITTFAG 401

BLAST of Sgr023648 vs. NCBI nr
Match: KAG7010712.1 (Indole-3-acetic acid-amido synthetase GH3.6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 565.1 bits (1455), Expect = 5.1e-157
Identity = 281/417 (67.39%), Postives = 332/417 (79.62%), Query Frame = 0

Query: 1   MSNGGENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDAS 60
           M++  E   DE+DKQALQHIEDV  +A +IQR++LT+ILSTNA VEYLQ++GL GST+ S
Sbjct: 15  MAHVDEISSDERDKQALQHIEDVTTHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 74

Query: 61  TFKKLIPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFA 120
           TFK LIP+++YEQL+PYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIP++EEEF 
Sbjct: 75  TFKNLIPLIAYEQLRPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPMYEEEFM 134

Query: 121 RRSSILNYVMPMMKQLFPG--LDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKN 180
           RR S  +YVMP MKQLFP   + KGKGM+F F + E  T +GIVA    TSLCKR++  N
Sbjct: 135 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTTSGIVAHTATTSLCKRANLNN 194

Query: 181 MPSGSYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVL 240
                 TSP DIILCTD  QSLY Q LCGLCQND V RVGA+FASTLIHV  FLE HWV+
Sbjct: 195 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDVVFRVGALFASTLIHVLKFLEDHWVV 254

Query: 241 LANDIRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYI 300
           LA+DIR GT+N KI+DPSVRE+VMNIL PN +LAD IE ECSKG WEGIITRLWPN KYI
Sbjct: 255 LASDIRAGTLNTKITDPSVRETVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 314

Query: 301 DAIVTGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYF 360
           +AIVTGSM+QY+P L+YYSN LP++ + YG+SE F GLNL+P   P+  SYT IPTMAYF
Sbjct: 315 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 374

Query: 361 EFLPIDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAGKTLILFI 416
           EFLPID           R +++ E+++ELVDLVDVKLG EYELV+TTYAGK++ L +
Sbjct: 375 EFLPID-----------RTKSSRELSRELVDLVDVKLGHEYELVITTYAGKSVCLLL 414

BLAST of Sgr023648 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 561.6 bits (1446), Expect = 5.6e-156
Identity = 279/410 (68.05%), Postives = 327/410 (79.76%), Query Frame = 0

Query: 1   MSNGGENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDAS 60
           M++  E   DE+DKQALQHIEDV  +A +IQR++LT+ILSTNA VEYLQ++GL GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKKLIPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFA 120
           TFK LIP++SYEQL+PYIDRIANGDDSPILC NPITEFFISSGTSGGEPKLIP++EEEF 
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRSSILNYVMPMMKQLFPG--LDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKN 180
           RR S  +YVMP MKQLFP   + KGKGM+F F + E  T++GIVA    TSLCKR++  N
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN 180

Query: 181 MPSGSYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVL 240
                 TSP DIILCTD  QSLY Q LCGLCQND V RVGA+FASTLIHV  FLE HWV+
Sbjct: 181 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYI 300
           LA+DIR GT+N KI+DPSVRE+VMNIL PN +LAD IE ECSKG WEGIITRLWPN KYI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 DAIVTGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYF 360
           +AIVTGSM+QY+P L+YYSN LP++ + YG+SE F GLNL+P   P+  SYT IPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           EFLPID           R +++ E+++ELVDLVDVKLG EYELV+TTYAG
Sbjct: 361 EFLPID-----------RTKSSRELSRELVDLVDVKLGHEYELVITTYAG 393

BLAST of Sgr023648 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 561.2 bits (1445), Expect = 7.4e-156
Identity = 279/410 (68.05%), Postives = 326/410 (79.51%), Query Frame = 0

Query: 1   MSNGGENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDAS 60
           M++  E   DE+DKQALQHIEDV  +A +IQR++LT+ILSTNA VEYLQ++GL GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKKLIPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFA 120
           TFK LIP++SYEQL+PYIDRIANGDDSPILC NPITEFFISSGTSGGEPKLIP++EEEF 
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRSSILNYVMPMMKQLFPG--LDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKN 180
           RR S  +YVMP MKQLFP   + KGKGM+F F + E  T++GIVA    TSLCKR++  N
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN 180

Query: 181 MPSGSYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVL 240
                 TSP DIILCTD  QSLY Q LCGLCQND V RVGA+FASTLIHV  FLE HWV+
Sbjct: 181 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYI 300
           LA+DIR GT+N KI+DPSVRE+VMNIL PN +LAD IE ECSKG WEGIITRLWPN KYI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 DAIVTGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYF 360
           +AIVTGSM+QY+P L+YYSN LP++ + YG SE F GLNL+P   P+  SYT IPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           EFLPID           R +++ E+++ELVDLVDVKLG EYELV+TTYAG
Sbjct: 361 EFLPID-----------RTKSSRELSRELVDLVDVKLGHEYELVITTYAG 393

BLAST of Sgr023648 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 527.7 bits (1358), Expect = 1.2e-148
Identity = 257/406 (63.30%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 6   ENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKL 65
           +  L EK+K  LQ IEDV  NAD++QRRVL EILS NA VEYL+R+GL G TD  TFK +
Sbjct: 14  DESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHI 73

Query: 66  IPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSI 125
           +PVV+YE ++P I+RIANGD S +LC+NPI+EF  SSGTSGGE KL+P  EEE  RRS +
Sbjct: 74  MPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLL 133

Query: 126 LNYVMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG--- 185
            + +MP+M Q  PGLDKGKGMYF F ++ES T  G+ ARP+ TS  K SHFKN P     
Sbjct: 134 YSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYT 193

Query: 186 SYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLAND 245
           +YTSPN  ILC+D YQS+Y Q LCGLCQ+ +VLRVGAVFAS  I    FLEKHW  LA D
Sbjct: 194 NYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARD 253

Query: 246 IRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIV 305
           IRTGT++ +I+D SVRE+V  ILKP+PKLAD +E EC K  W+GIITRLWPNTKY+D IV
Sbjct: 254 IRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIV 313

Query: 306 TGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP LDYYSNGLPL+C  Y SSE +FG+NL P  KP E+SYT IP MAYFEFLP
Sbjct: 314 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLP 373

Query: 366 IDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           + R +G     +       +  +ELVDLVDVKLGQEYELVVTTYAG
Sbjct: 374 VHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 419

BLAST of Sgr023648 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 1.0e-147
Identity = 250/403 (62.03%), Postives = 306/403 (75.93%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPV 68
           LD+K+KQ LQ IE++  NAD++QR+VL EIL+ NA VEYL+R+ L+G TD  TFK ++PV
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  VSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNY 128
           ++YE ++P I+RIANGD SPIL + PI+EF  SSGTSGGE KL+P  EEE  RRS + + 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG---SYT 188
           +MP+M Q  PGL+ GKGMYF F ++ES T  G+ ARP+ TS  K SHFK  P     +YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRT 248
           SPN+ ILC+D YQS+Y Q LCGLCQ+ +VLRVGAVFAS  I    FLEKHW+ L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGS 308
           GT++  I+DPSVRE+V  ILKP+PKLAD +E+EC K  W+GIITRLWPNTKY+D IVTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDR 368
           MSQYIP LDYYSNGLPL+C  Y SSE +FG+NL P  KP E+SYT IP+MAYFEFLP+ R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 GNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
            NG  +          +  +ELVDLVDVKLGQEYELVVTTYAG
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 419

BLAST of Sgr023648 vs. ExPASy Swiss-Prot
Match: A3BLS0 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. indica OX=39946 GN=GH3.8 PE=1 SV=2)

HSP 1 Score: 445.3 bits (1144), Expect = 7.7e-124
Identity = 214/401 (53.37%), Postives = 277/401 (69.08%), Query Frame = 0

Query: 11  EKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPVVS 70
           E D + L+ I+++  N D +Q RVL EIL  NA  EYL + GL G+TD + F+  +PVVS
Sbjct: 24  EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83

Query: 71  YEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNYVM 130
           Y+ L+PYI RIANGD SPIL  +P++EF  SSGTS GE KL+P   +E  RR  + + +M
Sbjct: 84  YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143

Query: 131 PMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG---SYTSP 190
           P+M    PGLDKGKG+YF F ++E+ T  G+ ARP+ TS  K  HFKN P     +YTSP
Sbjct: 144 PVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSP 203

Query: 191 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 250
              ILC D +QS+Y Q +CGLCQ + VLR+GAVFAS L+    FL+ +W  LA+DI +G 
Sbjct: 204 TAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGE 263

Query: 251 INPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSMS 310
           + P+++DPSVRE+V  IL P+P+LA LI  ECSKG W GIITR+WPNTKY+D IVTG+M+
Sbjct: 264 LTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMA 323

Query: 311 QYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRGN 370
           QYIP L++YS GLP+ C  Y SSE +FGLNL P   P E+SYT +P M YFEFLP+D   
Sbjct: 324 QYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG 383

Query: 371 GFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
             + D           A +LVDL  V++G+EYELV+TTYAG
Sbjct: 384 AASGD-----------ATQLVDLARVEVGREYELVITTYAG 413

BLAST of Sgr023648 vs. ExPASy Swiss-Prot
Match: Q0D4Z6 (Indole-3-acetic acid-amido synthetase GH3.8 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.8 PE=1 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 7.7e-124
Identity = 214/401 (53.37%), Postives = 277/401 (69.08%), Query Frame = 0

Query: 11  EKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPVVS 70
           E D + L+ I+++  N D +Q RVL EIL  NA  EYL + GL G+TD + F+  +PVVS
Sbjct: 24  EGDVEKLRFIDEMTTNVDAVQERVLGEILGRNAGTEYLTKCGLDGATDRAAFRAKVPVVS 83

Query: 71  YEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNYVM 130
           Y+ L+PYI RIANGD SPIL  +P++EF  SSGTS GE KL+P   +E  RR  + + +M
Sbjct: 84  YDDLQPYIQRIANGDRSPILSTHPVSEFLTSSGTSAGERKLMPTIMDELDRRQLLYSLLM 143

Query: 131 PMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG---SYTSP 190
           P+M    PGLDKGKG+YF F ++E+ T  G+ ARP+ TS  K  HFKN P     +YTSP
Sbjct: 144 PVMNLYVPGLDKGKGLYFLFVKSETKTPGGLTARPVLTSYYKSDHFKNRPYDPYHNYTSP 203

Query: 191 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 250
              ILC D +QS+Y Q +CGLCQ + VLR+GAVFAS L+    FL+ +W  LA+DI +G 
Sbjct: 204 TAAILCADAFQSMYAQMVCGLCQRNDVLRLGAVFASGLLRAIRFLQLNWEQLADDIESGE 263

Query: 251 INPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSMS 310
           + P+++DPSVRE+V  IL P+P+LA LI  ECSKG W GIITR+WPNTKY+D IVTG+M+
Sbjct: 264 LTPRVTDPSVREAVAAILLPDPELAKLIRAECSKGDWAGIITRVWPNTKYLDVIVTGAMA 323

Query: 311 QYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRGN 370
           QYIP L++YS GLP+ C  Y SSE +FGLNL P   P E+SYT +P M YFEFLP+D   
Sbjct: 324 QYIPTLEFYSGGLPMACTMYASSECYFGLNLRPMCDPSEVSYTIMPNMGYFEFLPVDETG 383

Query: 371 GFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
             + D           A +LVDL  V++G+EYELV+TTYAG
Sbjct: 384 AASGD-----------ATQLVDLARVEVGREYELVITTYAG 413

BLAST of Sgr023648 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 5.0e-123
Identity = 217/404 (53.71%), Postives = 276/404 (68.32%), Query Frame = 0

Query: 8   PLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIP 67
           P  EKD +AL+ IE++ RNAD +Q  +L EIL+ NA  EYL+R+ L G+TD  TFK  IP
Sbjct: 14  PACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIP 73

Query: 68  VVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILN 127
           V++YE L+P I RIA+GD SPIL A+PI+EF  SSGTS GE KL+P  +EE  RR  + +
Sbjct: 74  VITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYS 133

Query: 128 YVMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNM---PSGSY 187
            +MP+M    PGLDKGKGMYF F ++E+ T  G+ ARP+ TS  K  HF++    P   Y
Sbjct: 134 LLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVY 193

Query: 188 TSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIR 247
           TSPN+ ILC D +QS+Y Q LCGL     VLRVGAVFAS L+    FL+ HW   A+DI 
Sbjct: 194 TSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIE 253

Query: 248 TGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTG 307
            G ++ +I+DPS+R+ +  ILKP+P LA+ I  EC    WE IITR+WPNTKY+D IVTG
Sbjct: 254 LGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTG 313

Query: 308 SMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPID 367
           +M+QYIP L+YYS GLP+ C  Y SSE +FGLNLNP SKP E+SYT +P MAYFEF+P+ 
Sbjct: 314 AMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG 373

Query: 368 RGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
                                + V+LVDV +G+EYELVVTTYAG
Sbjct: 374 -------------------GTKAVELVDVNIGKEYELVVTTYAG 398

BLAST of Sgr023648 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.4e-163
Identity = 289/401 (72.07%), Postives = 326/401 (81.30%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPV 68
           LDEKDKQALQ +EDV R ADEIQRRVLTEILSTNAHVEYLQ++GL+GSTDASTFK LIP+
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  VSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNY 128
           VSYE LKPYIDRI NGDDSPILCA+PITEF+ S+GTSGGE KLIP  E+EFAR+ SI NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMP-SGSYTSP 188
           + P MKQLFPGL  GK M+F F + ES TKAGIVAR + TS  K  H  N+    SYTSP
Sbjct: 124 MRPRMKQLFPGLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTSP 183

Query: 189 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 248
            DI+ CTD+YQSLY Q LCGLCQN KV+RVG++FASTLI++F FLE HWVLLANDIRTGT
Sbjct: 184 TDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTGT 243

Query: 249 INPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSMS 308
           IN KI D  VR+SVMNILKP+P+LAD IE ECSKG WEGI+TRLWPNTK+I+A+VTGSMS
Sbjct: 244 INAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSMS 303

Query: 309 QYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRGN 368
            YIP L+YY   LPLI   Y SSE FFG+NL+P  KPEEISYTFIPT+AYFEFLP+DR N
Sbjct: 304 HYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRTN 363

Query: 369 GFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
                       + EV +E VDLVDVKLGQEYE+VVTTYAG
Sbjct: 364 D-----------SGEVGQEFVDLVDVKLGQEYEIVVTTYAG 393

BLAST of Sgr023648 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.7e-156
Identity = 279/410 (68.05%), Postives = 327/410 (79.76%), Query Frame = 0

Query: 1   MSNGGENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDAS 60
           M++  E   DE+DKQALQHIEDV  +A +IQR++LT+ILSTNA VEYLQ++GL GST+ S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKKLIPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFA 120
           TFK LIP++SYEQL+PYIDRIANGDDSPILC NPITEFFISSGTSGGEPKLIP++EEEF 
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRSSILNYVMPMMKQLFPG--LDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKN 180
           RR S  +YVMP MKQLFP   + KGKGM+F F + E  T++GIVA    TSLCKR++  N
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANLNN 180

Query: 181 MPSGSYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVL 240
                 TSP DIILCTD  QSLY Q LCGLCQND V RVGA+FASTLIHV  FLE HWV+
Sbjct: 181 ------TSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYI 300
           LA+DIR GT+N KI+DPSVRE+VMNIL PN +LAD IE ECSKG WEGIITRLWPN KYI
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 DAIVTGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYF 360
           +AIVTGSM+QY+P L+YYSN LP++ + YG+SE F GLNL+P   P+  SYT IPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           EFLPID           R +++ E+++ELVDLVDVKLG EYELV+TTYAG
Sbjct: 361 EFLPID-----------RTKSSRELSRELVDLVDVKLGHEYELVITTYAG 393

BLAST of Sgr023648 vs. ExPASy TrEMBL
Match: A0A5B6ZY21 (Putative indole-3-acetic acid-amido synthetase GH3.6 OS=Davidia involucrata OX=16924 GN=Din_018680 PE=3 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.8e-155
Identity = 266/401 (66.33%), Postives = 315/401 (78.55%), Query Frame = 0

Query: 11  EKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPVVS 70
           EK+K+ LQ IEDV  NADE+Q+RVL EILS NA V+YLQR+GL G TD  TFKKL+PV++
Sbjct: 20  EKNKKVLQFIEDVTANADEVQKRVLAEILSRNARVDYLQRHGLGGHTDRETFKKLMPVIT 79

Query: 71  YEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNYVM 130
           YE ++P I+RIANGD SPILC+ PI+EF  SSGTSGGE KL+P  EEE  RRS + + +M
Sbjct: 80  YEDIQPDINRIANGDTSPILCSQPISEFLTSSGTSGGERKLMPTIEEELGRRSLLYSLLM 139

Query: 131 PMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG---SYTSP 190
           P+M Q  PGLDKGKGMYF F ++E+ T +G+VARP+ TS  K +HFKN P     +YTSP
Sbjct: 140 PVMNQSVPGLDKGKGMYFLFIKSEAKTPSGLVARPVLTSYYKSAHFKNRPYDPYTNYTSP 199

Query: 191 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 250
           N+ ILC D YQS+Y Q LCGLCQN +VLRVGAVFAS  I    FLEKHW LL  DIRTGT
Sbjct: 200 NETILCPDSYQSMYSQMLCGLCQNSEVLRVGAVFASGFIRAIRFLEKHWTLLCKDIRTGT 259

Query: 251 INPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSMS 310
           INP+I+DPSVRE+VM I++P+PKLAD +E ECSK  W+GIITRLWPNTKY+D IVTG+MS
Sbjct: 260 INPQITDPSVREAVMKIIRPDPKLADFVEAECSKESWQGIITRLWPNTKYVDVIVTGTMS 319

Query: 311 QYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRGN 370
           QYIP LDYYSNGLPL+C  Y SSE +FG+NLNP  KP ++SYT IPTMAYFEFLP+ R N
Sbjct: 320 QYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSDVSYTLIPTMAYFEFLPVHRNN 379

Query: 371 GFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           G  +  T     N +  +ELVDLVDVKLGQEYELVVTTYAG
Sbjct: 380 GVTNSITVPKSLNEKEQQELVDLVDVKLGQEYELVVTTYAG 420

BLAST of Sgr023648 vs. ExPASy TrEMBL
Match: A0A6J1CG47 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111011212 PE=3 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 3.0e-155
Identity = 267/401 (66.58%), Postives = 319/401 (79.55%), Query Frame = 0

Query: 11  EKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPVVS 70
           EK ++ALQ+IE V  +AD++QRRVLTEILS NAHVEYL R+GL G  D  TFKK +PVVS
Sbjct: 21  EKHERALQYIEQVTAHADQVQRRVLTEILSANAHVEYL-RHGLDGYMDTKTFKKTMPVVS 80

Query: 71  YEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNYVM 130
           Y+ +KPY+DRIANGD SPILC+ PI+EF  SSGTSGGE KL+P  EEE  R+S + + +M
Sbjct: 81  YDDIKPYVDRIANGDPSPILCSKPISEFLTSSGTSGGERKLMPTTEEELERKSLLYSLLM 140

Query: 131 PMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNM---PSGSYTSP 190
           P+M Q  PGLDKGKGMYF F ++ES T  G++ARP+ TS  +  HFK+    P   YTSP
Sbjct: 141 PVMNQFIPGLDKGKGMYFLFVKSESTTPGGLLARPVLTSYYRSPHFKDRPYDPYNQYTSP 200

Query: 191 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 250
           N+ ILCTD YQS+Y Q+LCGLC + +VLRVGAVFAS  I    FLE HW+LL+NDIRTGT
Sbjct: 201 NETILCTDSYQSMYSQFLCGLCLSREVLRVGAVFASGFIRAIKFLETHWLLLSNDIRTGT 260

Query: 251 INPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSMS 310
           INP+I+DP VR+SV+NILKP+P+LADLIE ECSKG WEGII+RLW NTKYID IVTG+MS
Sbjct: 261 INPRITDPLVRQSVLNILKPDPELADLIESECSKGTWEGIISRLWRNTKYIDVIVTGTMS 320

Query: 311 QYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRGN 370
           QYIP LDYYSNGLPL+C  Y SSE +FG+NLNP   P E+SYT IPTMAYFEFLP+D  N
Sbjct: 321 QYIPTLDYYSNGLPLVCTMYASSECYFGINLNPLCNPSEVSYTLIPTMAYFEFLPLDGTN 380

Query: 371 GFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           GF    TC+ EAN +  +ELVDLVDV+LG+EYELV+TTYAG
Sbjct: 381 GFGYSSTCQSEANQKTDQELVDLVDVQLGKEYELVITTYAG 420

BLAST of Sgr023648 vs. ExPASy TrEMBL
Match: A0A6P3YWT6 (indole-3-acetic acid-amido synthetase GH3.6-like isoform X1 OS=Ziziphus jujuba OX=326968 GN=LOC107404357 PE=3 SV=1)

HSP 1 Score: 551.6 bits (1420), Expect = 2.8e-153
Identity = 269/410 (65.61%), Postives = 314/410 (76.59%), Query Frame = 0

Query: 6   ENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKL 65
           E  L EK+K+ LQ IEDV  NADE+Q+RVL EILS NAHVEYLQRYGL+G TD  TFKKL
Sbjct: 13  EYNLAEKNKKTLQFIEDVTENADEVQKRVLAEILSCNAHVEYLQRYGLNGHTDRDTFKKL 72

Query: 66  IPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSI 125
           IPV++YE ++P I+RIANGD S ILC +PI+EF  SSGTSGGE KL+P  EEE  RRS +
Sbjct: 73  IPVITYEDIQPDINRIANGDTSQILCCSPISEFLTSSGTSGGERKLMPTIEEELGRRSLL 132

Query: 126 LNYVMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG--- 185
            + +MP+M Q  PGLD+GKGMYF F ++E+ T  G+VARP+ TS  K SHFK  P     
Sbjct: 133 YSLLMPVMNQFVPGLDQGKGMYFLFIKSEAKTPGGLVARPVLTSYYKSSHFKERPFDPYT 192

Query: 186 SYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLAND 245
           +YTSPN+ ILC D YQS+Y Q LCGLCQN +VLRVGAVFAS  I    FLEKHW LL ND
Sbjct: 193 NYTSPNETILCQDSYQSMYSQMLCGLCQNKEVLRVGAVFASGFIRAIRFLEKHWPLLCND 252

Query: 246 IRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIV 305
           IR GTIN +I+DPS+RESVM ILKP+PKLAD IE EC+K  W+GIITRLWPNTKY+D IV
Sbjct: 253 IRNGTINDQITDPSIRESVMKILKPDPKLADFIEMECAKDSWQGIITRLWPNTKYVDVIV 312

Query: 306 TGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP LDYYSNGLPL+C  Y SSE +FG+NLNP  KP E+SYT IPTMAYFEFLP
Sbjct: 313 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVSYTLIPTMAYFEFLP 372

Query: 366 IDRGNGF----NDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           + R NG      +  +     N +  +ELVDLV+VKLG EYELVVTTYAG
Sbjct: 373 VHRNNGVVSTTTNSLSVAKSLNEKEQQELVDLVNVKLGHEYELVVTTYAG 422

BLAST of Sgr023648 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 527.7 bits (1358), Expect = 8.4e-150
Identity = 257/406 (63.30%), Postives = 305/406 (75.12%), Query Frame = 0

Query: 6   ENPLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKL 65
           +  L EK+K  LQ IEDV  NAD++QRRVL EILS NA VEYL+R+GL G TD  TFK +
Sbjct: 14  DESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHI 73

Query: 66  IPVVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSI 125
           +PVV+YE ++P I+RIANGD S +LC+NPI+EF  SSGTSGGE KL+P  EEE  RRS +
Sbjct: 74  MPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLL 133

Query: 126 LNYVMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG--- 185
            + +MP+M Q  PGLDKGKGMYF F ++ES T  G+ ARP+ TS  K SHFKN P     
Sbjct: 134 YSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYT 193

Query: 186 SYTSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLAND 245
           +YTSPN  ILC+D YQS+Y Q LCGLCQ+ +VLRVGAVFAS  I    FLEKHW  LA D
Sbjct: 194 NYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARD 253

Query: 246 IRTGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIV 305
           IRTGT++ +I+D SVRE+V  ILKP+PKLAD +E EC K  W+GIITRLWPNTKY+D IV
Sbjct: 254 IRTGTLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIV 313

Query: 306 TGSMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLP 365
           TG+MSQYIP LDYYSNGLPL+C  Y SSE +FG+NL P  KP E+SYT IP MAYFEFLP
Sbjct: 314 TGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLP 373

Query: 366 IDRGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
           + R +G     +       +  +ELVDLVDVKLGQEYELVVTTYAG
Sbjct: 374 VHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 419

BLAST of Sgr023648 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 524.6 bits (1350), Expect = 7.1e-149
Identity = 250/403 (62.03%), Postives = 306/403 (75.93%), Query Frame = 0

Query: 9   LDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPV 68
           LD+K+KQ LQ IE++  NAD++QR+VL EIL+ NA VEYL+R+ L+G TD  TFK ++PV
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  VSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNY 128
           ++YE ++P I+RIANGD SPIL + PI+EF  SSGTSGGE KL+P  EEE  RRS + + 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNMPSG---SYT 188
           +MP+M Q  PGL+ GKGMYF F ++ES T  G+ ARP+ TS  K SHFK  P     +YT
Sbjct: 137 LMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNYT 196

Query: 189 SPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRT 248
           SPN+ ILC+D YQS+Y Q LCGLCQ+ +VLRVGAVFAS  I    FLEKHW+ L  DIRT
Sbjct: 197 SPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIRT 256

Query: 249 GTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGS 308
           GT++  I+DPSVRE+V  ILKP+PKLAD +E+EC K  W+GIITRLWPNTKY+D IVTG+
Sbjct: 257 GTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTGT 316

Query: 309 MSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDR 368
           MSQYIP LDYYSNGLPL+C  Y SSE +FG+NL P  KP E+SYT IP+MAYFEFLP+ R
Sbjct: 317 MSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVHR 376

Query: 369 GNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
            NG  +          +  +ELVDLVDVKLGQEYELVVTTYAG
Sbjct: 377 NNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAG 419

BLAST of Sgr023648 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 442.6 bits (1137), Expect = 3.6e-124
Identity = 217/404 (53.71%), Postives = 276/404 (68.32%), Query Frame = 0

Query: 8   PLDEKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIP 67
           P  EKD +AL+ IE++ RNAD +Q  +L EIL+ NA  EYL+R+ L G+TD  TFK  IP
Sbjct: 14  PACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIP 73

Query: 68  VVSYEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILN 127
           V++YE L+P I RIA+GD SPIL A+PI+EF  SSGTS GE KL+P  +EE  RR  + +
Sbjct: 74  VITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYS 133

Query: 128 YVMPMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNM---PSGSY 187
            +MP+M    PGLDKGKGMYF F ++E+ T  G+ ARP+ TS  K  HF++    P   Y
Sbjct: 134 LLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVY 193

Query: 188 TSPNDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIR 247
           TSPN+ ILC D +QS+Y Q LCGL     VLRVGAVFAS L+    FL+ HW   A+DI 
Sbjct: 194 TSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIE 253

Query: 248 TGTINPKISDPSVRESVMNILKPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTG 307
            G ++ +I+DPS+R+ +  ILKP+P LA+ I  EC    WE IITR+WPNTKY+D IVTG
Sbjct: 254 LGCLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTG 313

Query: 308 SMSQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPID 367
           +M+QYIP L+YYS GLP+ C  Y SSE +FGLNLNP SKP E+SYT +P MAYFEF+P+ 
Sbjct: 314 AMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLG 373

Query: 368 RGNGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
                                + V+LVDV +G+EYELVVTTYAG
Sbjct: 374 -------------------GTKAVELVDVNIGKEYELVVTTYAG 398

BLAST of Sgr023648 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 433.3 bits (1113), Expect = 2.2e-121
Identity = 214/402 (53.23%), Postives = 281/402 (69.90%), Query Frame = 0

Query: 11  EKDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPVVS 70
           EKD +AL+ IE++ RN D +Q +VL EIL+ N++ EYL+R+ L G  D  TFK  +PVV+
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 71  YEQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNYVM 130
           YE LKP I RI+NGD SPIL ++PITEF  SSGTS GE KL+P  EE+  RR  + + +M
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 131 PMMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFKNM---PSGSYTSP 190
           P+M    PGLDKGKG+YF F ++ES T  G+ ARP+ TS  K  HFK     P   YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 191 NDIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGT 250
           N+ ILC+D  QS+Y Q LCGL    +VLR+GAVFAS L+   +FL+ +W  LA DI TGT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 251 INPKISDPSVRESVMNIL-KPNPKLADLIEYECSKGMWEGIITRLWPNTKYIDAIVTGSM 310
           ++ +I DP+++  +  IL KP+ +LA+ +   CS+  WEGIIT++WPNTKY+D IVTG+M
Sbjct: 258 LSSRIFDPAIKNRMSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGAM 317

Query: 311 SQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRG 370
           +QYIP L+YYS GLP+ C  Y SSES+FG+NL P  KP E+SYT +P MAYFEFLP    
Sbjct: 318 AQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP---- 377

Query: 371 NGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
              N D     EA+ +    LV+L +V++G+EYELV+TTYAG
Sbjct: 378 --HNHDGDGAAEASLD-ETSLVELANVEVGKEYELVITTYAG 412

BLAST of Sgr023648 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 428.3 bits (1100), Expect = 6.9e-120
Identity = 217/402 (53.98%), Postives = 276/402 (68.66%), Query Frame = 0

Query: 12  KDKQALQHIEDVMRNADEIQRRVLTEILSTNAHVEYLQRYGLSGSTDASTFKKLIPVVSY 71
           KD +AL+ IE++ RN D +Q++V+ EILS N+  EYL+R+GL G TD  TFK  +PVV Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYIDRIANGDDSPILCANPITEFFISSGTSGGEPKLIPIFEEEFARRSSILNYVMP 131
           + LKP I RIANGD S IL + PITEF  SSGTS GE KL+P  +E+  RR  + + +MP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 MMKQLFPGLDKGKGMYFGFTRAESWTKAGIVARPMFTSLCKRSHFK---NMPSGSYTSPN 191
           +M    PGLDKGK +YF F + ES T  G+ ARP+ TS  K   FK   N P   YTSPN
Sbjct: 138 VMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNVYTSPN 197

Query: 192 DIILCTDLYQSLYGQWLCGLCQNDKVLRVGAVFASTLIHVFNFLEKHWVLLANDIRTGTI 251
           + ILC D  QS+Y Q LCGL    +VLR+GAVFAS L+    FL+ +W  LA+DI TGT+
Sbjct: 198 EAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDISTGTL 257

Query: 252 NPKISDPSVRESVMNIL-KPNPKLADLIEYECSK-GMWEGIITRLWPNTKYIDAIVTGSM 311
           + +ISDP+++ES+  IL KP+ +LAD I   C +   WEGIIT++WPNTKY+D IVTG+M
Sbjct: 258 SSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIVTGAM 317

Query: 312 SQYIPMLDYYSNGLPLICNSYGSSESFFGLNLNPSSKPEEISYTFIPTMAYFEFLPIDRG 371
           +QYIPML+YYS GLP+ C  Y SSES+FG+NL P  KP E+SYT +P MAYFEFLP    
Sbjct: 318 AQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP---- 377

Query: 372 NGFNDDYTCRPEANAEVAKELVDLVDVKLGQEYELVVTTYAG 409
                      E   E   ELV+L DV++G+EYELV+TTYAG
Sbjct: 378 ---------HHEVPTE-KSELVELADVEVGKEYELVITTYAG 405