Sgr023386 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023386 vs. NCBI nr
Match: XP_022146221.1 (uncharacterized protein LOC111015485 [Momordica charantia])

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 684/1117 (61.24%), Postives = 784/1117 (70.19%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISL+EVAGEDD LL  IPE++LLNLDKDTD +TAGNSGFFLCSPLLTDRS+GTVAG
Sbjct: 1    MESEISLLEVAGEDDFLLQQIPEDDLLNLDKDTDRITAGNSGFFLCSPLLTDRSNGTVAG 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS ASSTD    C+  DKEN+N NNLEVPKLSIM QQMK+KKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASSTD----CT--DKENINVNNLEVPKLSIMSQQMKKKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG TSTSCGEALAAIDEE+PAMPGGGC +D+SS DK F D S STP
Sbjct: 121  TEEGVLDSMELSMITGSTSTSCGEALAAIDEEIPAMPGGGCYNDLSSQDKLFTDASISTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
              DRKNGR LLPKRGS+TKDNVKSKELSTK+ NRS SKRGSCPRPVASS    PTTS GT
Sbjct: 181  SADRKNGRCLLPKRGSTTKDNVKSKELSTKNSNRSVSKRGSCPRPVASSSTKRPTTSKGT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTRSTTIRASDAKSNQVAQRGVG--NNPKQTTLKSS 356
            KTV K+ERISR+ V KRDP VTS T R+ T  AS+AK+NQVAQRGVG  NNPK+TTLK S
Sbjct: 241  KTVNKEERISRVLVPKRDPAVTSWTPRNATTHASNAKNNQVAQRGVGVVNNPKRTTLKGS 300

Query: 357  SINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLKAATNS 416
            +IN KSAL+KDVNA+K+LKAKTSIQQPRRKLASPV+KM+SSHL NESTDSNE LKAA+ S
Sbjct: 301  AINTKSALHKDVNANKSLKAKTSIQQPRRKLASPVIKMSSSHLQNESTDSNEELKAASKS 360

Query: 417  LISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQKKVSS 476
            LISK +P NDDGTRKVSAS+TQNA P GG ML+QTQMPKPSGLRMPSPSMGFFGQKKVSS
Sbjct: 361  LISKFIPFNDDGTRKVSASITQNAPPTGGSMLSQTQMPKPSGLRMPSPSMGFFGQKKVSS 420

Query: 477  FQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGETNVVSC 536
            FQ+L PATSE H LSKS +PN+RTAGPSN  CQLA  L P NFVKAS+ EASGETNVVSC
Sbjct: 421  FQNLAPATSETHDLSKSYIPNVRTAGPSNYTCQLA-ALAPTNFVKASNGEASGETNVVSC 480

Query: 537  LSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLSSR--- 596
             SSG SV+P+SH RAKS+ +AA IHLGKVNV GAST N DLSAHGLEKPD+LSLS+    
Sbjct: 481  FSSGISVQPVSHYRAKSISEAANIHLGKVNVVGASTMNNDLSAHGLEKPDLLSLSNPVLQ 540

Query: 597  --GDVSRYMMRLTTNLQN-----------ANTVMCHL----------------------- 656
              GDVSR    +   L               +   HL                       
Sbjct: 541  NFGDVSRIHDEIKVQLAECKPRHLSFNNFGESTKSHLGEKNGLGSQGLPRALSNGSQDFN 600

Query: 657  ---TILLTLPN--HILVKRTTWAHKRLGVGTCNSLKRSRTSKDFEH-GRFEENVGNDSNG 716
               +  +  PN    +++R +   +R G+G+CNSLKRSR+S D EH G FEEN+GN S  
Sbjct: 601  EPSSEQVEFPNSPKCILERISSDSQRSGIGSCNSLKRSRSSIDLEHGGSFEENIGNGS-- 660

Query: 717  QECYAFDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SM 776
                AF+KD+NEGQDE   T +MR+L T+E E S +DHCISNE               SM
Sbjct: 661  ---CAFEKDVNEGQDETLGTRRMRILRTKEVESSGIDHCISNECKNTIQSTSAWSNSDSM 720

Query: 777  HIDDEKPTVPMSNNTS----GFSLTSQHDYAS-KNDILAEGSNGV--------------- 836
            HIDDEKPT+PMSN+ S    G SLTS HD+ S +N+ILA  SNGV               
Sbjct: 721  HIDDEKPTIPMSNSKSLQREGSSLTSHHDFNSHENEILAGVSNGVGENNLKSEIDLCLIQ 780

Query: 837  ---SDACLDSGPVVRNCNNHTDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAE 896
               +DACLD GPVVRNCN+HTD+M DI S+IEQN+T LE +RN D +GDVAIA N DAAE
Sbjct: 781  HSTADACLDIGPVVRNCNDHTDEMADISSNIEQNNTDLEMKRNLDDYGDVAIARNTDAAE 840

Query: 897  TFLITRDLCSSDTENQLYEAQMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCI 956
            T  +  DLC+SDTENQLYEAQ+ IGSE V NED QNSHV SSVN+FN+LP    LQN C 
Sbjct: 841  TLPLNPDLCTSDTENQLYEAQIQIGSEQVPNEDPQNSHVQSSVNDFNQLP---GLQNRCT 900

Query: 957  DLVEVSQNNNQGKCSNDDLLHTSNSEGYKEETITDSLIASSDCLRRLGISKCSTAISSEC 1016
            DLV+VSQNN+QGKCSNDD LHTSNSE YK+E IT+ +I  ++            A  +  
Sbjct: 901  DLVDVSQNNHQGKCSNDD-LHTSNSEEYKDEIITNGVIDYTN----------QVASHNNL 960

Query: 1017 EKVRSGEGAYETMSIEITSEARTTCNDLAFCWLTKELDSSVPNDHILSRENVQQCMETKE 1076
            E        ++ +S    S   +T N                     S E+ + C     
Sbjct: 961  E--------FQEISASARSSKHSTAN---------------------SSEHTETCS---- 1020

Query: 1077 LENHKSPQMNGNILCQNENELNSEWSIYQALKRAKKVRGCQKQNALGIKTSTNAVPFSEE 1086
                          C +           Q++++  K    +KQ   G +T TNAVPFSEE
Sbjct: 1021 -------------TCDDN---------AQSMQQLTKSEDVRKQ---GGRTETNAVPFSEE 1033

BLAST of Sgr023386 vs. NCBI nr
Match: XP_022986753.1 (uncharacterized protein LOC111484416 isoform X3 [Cucurbita maxima])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 694/1210 (57.36%), Postives = 798/1210 (65.95%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE++LL+L+++ DG TA NSGFFLCSPLLT+RS+GT++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDDLLDLEREMDGATARNSGFFLCSPLLTNRSNGTISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L A+DEE+PA+P GG  +D+S  DK FK+TSTSTP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAVDEEIPAVPSGGSYNDLSFQDKLFKNTSTSTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRG------VGNNPKQT 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQRG      V +NPK T
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQRGHQVAQRVVSNPKMT 300

Query: 357  TLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLK 416
            T   SS+NA  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEGLK
Sbjct: 301  TPNGSSMNAIRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEGLK 360

Query: 417  AATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQ 476
            A  NSLISK LP NDDGT+KVSAS+TQNA  +G  MLN TQMPKPSGLRMPSPSMGFFGQ
Sbjct: 361  ARENSLISKPLPSNDDGTKKVSASITQNAPHNGRSMLNPTQMPKPSGLRMPSPSMGFFGQ 420

Query: 477  KKVSSFQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGET 536
            KKVSSFQS+PP TSE+H LSKSN+PN+R  GPSNPICQLA TLVPRN VKA+  EASGET
Sbjct: 421  KKVSSFQSVPPDTSELHDLSKSNIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASGET 480

Query: 537  NVVSCLSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLS 596
             VVSCLSSGSS+EP+SHDRAKS LK A IHLGKV+V GAST  K LSAHGL KPDVLSLS
Sbjct: 481  KVVSCLSSGSSIEPLSHDRAKSALKVANIHLGKVDVIGASTMKKALSAHGLVKPDVLSLS 540

Query: 597  -----SRGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI----------- 656
                 + GDVSR    +   L   +T          C   +   L + +           
Sbjct: 541  NPVLENLGDVSRKHHEIKDQLAGDSTKSYLDETNESCSQGMQNALDDQLRGAKDCNEQSS 600

Query: 657  -----------LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQECYA 716
                        ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E  +
Sbjct: 601  EQVEVANSFNCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGTFED-VSNDSNGWESCS 660

Query: 717  FDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHIDDE 776
            FD       DE  ET KMR+L T + E SDVDH ISNE               SMHIDDE
Sbjct: 661  FD------LDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHIDDE 720

Query: 777  KPTVPMSNNTS----GFSLTSQHDYASKNDILAEGSNGVSDACLDSGPVVRN-------- 836
             PT P+ NN S    G SL      AS+N++L + SN + +  +DS   + +        
Sbjct: 721  NPTGPILNNKSLQGNGCSL------ASQNEVLTKESNDICENKVDSDYELSSIPHSTADA 780

Query: 837  CNNHTDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQ 896
            CN +TD++VDI SD++QN TSLETER Q+  GDV IA NAD AET LI+RD  SSDTENQ
Sbjct: 781  CNKYTDEIVDIVSDMQQNHTSLETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQ 840

Query: 897  LYEAQMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSN 956
            LYEA + I SEHVQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS 
Sbjct: 841  LYEAHIRIESEHVQNEDKQNSFVQSSVND------------CCIDLVDVSPKNNQGKCSI 900

Query: 957  DDLLHTSNSE-------------------------------GYKEET----ITDSLIASS 1016
            D LLH S+SE                                 +EET      D+++ S 
Sbjct: 901  DHLLHRSSSEEIITGSVDNSDVCTSEGLSNCNQMASPKDNSSIQEETHDTRTGDNILESQ 960

Query: 1017 DCLRRLGISKCSTAISSECEK-VRSGEGAYETMSIEITSEARTTCND------------- 1076
            +    +  S CSTA SSE +K + SGEG  ETMS +ITSE+R TCND             
Sbjct: 961  EIEASISSSSCSTAKSSEYDKIILSGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSS 1020

Query: 1077 -------------------------------------------------LAFCWLTKELD 1086
                                                              +FC  TK+L 
Sbjct: 1021 IPIDDTLSSENVQQYVGTKELDNHKSPEMNEMCSTYNDNAQSEATTCNVSSFCSPTKDLG 1080

BLAST of Sgr023386 vs. NCBI nr
Match: XP_022986754.1 (uncharacterized protein LOC111484416 isoform X4 [Cucurbita maxima])

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 694/1209 (57.40%), Postives = 796/1209 (65.84%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE++LL+L+++ DG TA NSGFFLCSPLLT+RS+GT++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDDLLDLEREMDGATARNSGFFLCSPLLTNRSNGTISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L A+DEE+PA+P GG  +D+S  DK FK+TSTSTP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAVDEEIPAVPSGGSYNDLSFQDKLFKNTSTSTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRG------VGNNPKQT 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQRG      V +NPK T
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQRGHQVAQRVVSNPKMT 300

Query: 357  TLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLK 416
            T   SS+NA  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEGLK
Sbjct: 301  TPNGSSMNAIRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEGLK 360

Query: 417  AATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQ 476
            A  NSLISK LP NDDGT+KVSAS+TQNA  +G  MLN TQMPKPSGLRMPSPSMGFFGQ
Sbjct: 361  ARENSLISKPLPSNDDGTKKVSASITQNAPHNGRSMLNPTQMPKPSGLRMPSPSMGFFGQ 420

Query: 477  KKVSSFQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGET 536
            KKVSSFQS+PP TSE+H LSKSN+PN+R  GPSNPICQLA TLVPRN VKA+  EASGET
Sbjct: 421  KKVSSFQSVPPDTSELHDLSKSNIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASGET 480

Query: 537  NVVSCLSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLS 596
             VVSCLSSGSS+EP+SHDRAKS LK A IHLGKV+V GAST  K LSAHGL KPDVLSLS
Sbjct: 481  KVVSCLSSGSSIEPLSHDRAKSALKVANIHLGKVDVIGASTMKKALSAHGLVKPDVLSLS 540

Query: 597  -----SRGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI----------- 656
                 + GDVSR    +   L   +T          C   +   L + +           
Sbjct: 541  NPVLENLGDVSRKHHEIKDQLAGDSTKSYLDETNESCSQGMQNALDDQLRGAKDCNEQSS 600

Query: 657  -----------LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQECYA 716
                        ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E  +
Sbjct: 601  EQVEVANSFNCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGTFED-VSNDSNGWESCS 660

Query: 717  FDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHIDDE 776
            FD       DE  ET KMR+L T + E SDVDH ISNE               SMHIDDE
Sbjct: 661  FD------LDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHIDDE 720

Query: 777  KPTVPMSNNTS----GFSLTSQHDYASKNDILAEGSNGVSDACLDSGPVVRN-------- 836
             PT P+ NN S    G SL      AS+N++L + SN + +  +DS   + +        
Sbjct: 721  NPTGPILNNKSLQGNGCSL------ASQNEVLTKESNDICENKVDSDYELSSIPHSTADA 780

Query: 837  CNNHTDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQ 896
            CN +TD++VDI SD++QN TSLETER Q+  GDV IA NAD AET LI+RD  SSDTENQ
Sbjct: 781  CNKYTDEIVDIVSDMQQNHTSLETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQ 840

Query: 897  LYEAQMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSN 956
            LYEA + I SEHVQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS 
Sbjct: 841  LYEAHIRIESEHVQNEDKQNSFVQSSVND------------CCIDLVDVSPKNNQGKCSI 900

Query: 957  DDLLHTSNSE-------------------------------GYKEET----ITDSLIASS 1016
            D LLH S+SE                                 +EET      D+++ S 
Sbjct: 901  DHLLHRSSSEEIITGSVDNSDVCTSEGLSNCNQMASPKDNSSIQEETHDTRTGDNILESQ 960

Query: 1017 DCLRRLGISKCSTAISSECEK-VRSGEGAYETMSIEITSEARTTCND------------- 1076
            +    +  S CSTA SSE +K + SGEG  ETMS +ITSE+R TCND             
Sbjct: 961  EIEASISSSSCSTAKSSEYDKIILSGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSS 1020

Query: 1077 -------------------------------------------------LAFCWLTKELD 1086
                                                              +FC  TK+L 
Sbjct: 1021 IPIDDTLSSENVQQYVGTKELDNHKSPEMNEMCSTYNDNAQSEATTCNVSSFCSPTKDLG 1080

BLAST of Sgr023386 vs. NCBI nr
Match: XP_022943291.1 (uncharacterized protein LOC111448106 isoform X3 [Cucurbita moschata])

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 703/1265 (55.57%), Postives = 793/1265 (62.69%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE+ LL+L+++ DG TA NSGFFLCSPLLT+RS G ++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDGLLDLEREMDGATARNSGFFLCSPLLTNRSIGAISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L AIDEE+PAMPGG   +D+S  DK FKDTST TP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAIDEEIPAMPGGSSYNDLSFQDKLFKDTSTGTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRGVGNNPKQTTLKSSS 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQR V +NPK +T KSSS
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQR-VVSNPKMSTSKSSS 300

Query: 357  INAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLKAATNSL 416
            +N+  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEGLKA  NSL
Sbjct: 301  MNSLRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEGLKAGANSL 360

Query: 417  ISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQKKVSSF 476
            ISK LP NDDGT+K SAS+TQNA PDG  MLN TQMPKPSGLRMPSPSMGFFGQKKVSSF
Sbjct: 361  ISKPLPSNDDGTKKASASITQNAPPDGRSMLNPTQMPKPSGLRMPSPSMGFFGQKKVSSF 420

Query: 477  QSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGETNVVSCL 536
            QS+PP TSE+H LSKS++PN+R  GPSNPICQLA TLVPRN VKA+  EASGET VVSCL
Sbjct: 421  QSVPPDTSELHDLSKSSIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASGETKVVSCL 480

Query: 537  SSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLSS----- 596
            SSGSS+EP+SH RAKS LK A IHLGKV+V GAST NK LSAHGL KPDVLSLS+     
Sbjct: 481  SSGSSIEPVSHYRAKSALKLANIHLGKVDVIGASTMNKALSAHGLVKPDVLSLSNPVLEH 540

Query: 597  RGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI----------------- 656
             GDVSR    +   L   +T          C   +   L + +                 
Sbjct: 541  LGDVSRTHHEIKDQLAGDSTKSYLDETNECCSQGMQNALDDQLRGAKDCNEQSSEQVEVA 600

Query: 657  -----LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQECYAFDKDMN 716
                  ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E  +FD    
Sbjct: 601  NSSYCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGIFED-VSNDSNGWESCSFD---- 660

Query: 717  EGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHIDDEKPTVPM 776
              QDE  ET KMR+L T + E SDVDH ISNE               SMHIDDE PT P+
Sbjct: 661  --QDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHIDDENPTGPI 720

Query: 777  SNNTS----GFSLTSQHDYASK--NDILAEGSNGVSDACLDSGPVVRNCNNHTDKMVDIR 836
             NN S    G+SL SQ+++ +K  NDI     +   D           CNN TD+MVDI 
Sbjct: 721  LNNQSLQGNGYSLASQNEFLTKESNDICENKVDSDYDLSSIPHSTADACNNCTDEMVDIV 780

Query: 837  SDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQLYEAQMCIGSEH 896
            SD++QN TS ETER Q+  GDV IA NAD AET LI+RD  SSDTENQLYEA + I SEH
Sbjct: 781  SDMQQNHTSFETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQLYEAHIRIESEH 840

Query: 897  VQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSNDDLLHTSNSE-- 956
            VQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS DDLLH SNSE  
Sbjct: 841  VQNEDKQNSLVQSSVND------------CCIDLVDVSPKNNQGKCSIDDLLHRSNSEEI 900

Query: 957  ---------------------------GYKEET----ITDSLIASSDCLRRLGISKCSTA 1016
                                          EET      D+++ S +    L  S+CSTA
Sbjct: 901  ISVDNYDVCTSEGLSNCNQMASPKDNNSIHEETHDTRTGDNILESRELEASLRSSRCSTA 960

Query: 1017 ISSECEKVRSGEGAYETMSIEITSEARTTCNDLAFC------------------------ 1076
             SSE +K  SGEG  ETMS +ITSE+R TCND +FC                        
Sbjct: 961  KSSEDDK--SGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSSIPNDDKLSSANVQQY 1020

Query: 1077 --------------------------------------WLTKELDSSVPNDHILSRENVQ 1086
                                                    TK+L SSVPND ILSRENVQ
Sbjct: 1021 AGTKELENHKSPEMNEMCSTYNDNAQSEATTCNDSSFRSPTKDLGSSVPNDDILSRENVQ 1080

BLAST of Sgr023386 vs. NCBI nr
Match: XP_022943290.1 (uncharacterized protein LOC111448106 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 704/1272 (55.35%), Postives = 794/1272 (62.42%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE+ LL+L+++ DG TA NSGFFLCSPLLT+RS G ++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDGLLDLEREMDGATARNSGFFLCSPLLTNRSIGAISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L AIDEE+PAMPGG   +D+S  DK FKDTST TP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAIDEEIPAMPGGSSYNDLSFQDKLFKDTSTGTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRG--------VGNNPK 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQRG        V +NPK
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQRGNHCKIAQTVVSNPK 300

Query: 357  QTTLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEG 416
             +T KSSS+N+  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEG
Sbjct: 301  MSTSKSSSMNSLRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEG 360

Query: 417  LKAATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFF 476
            LKA  NSLISK LP NDDGT+K SAS+TQNA PDG  MLN TQMPKPSGLRMPSPSMGFF
Sbjct: 361  LKAGANSLISKPLPSNDDGTKKASASITQNAPPDGRSMLNPTQMPKPSGLRMPSPSMGFF 420

Query: 477  GQKKVSSFQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASG 536
            GQKKVSSFQS+PP TSE+H LSKS++PN+R  GPSNPICQLA TLVPRN VKA+  EASG
Sbjct: 421  GQKKVSSFQSVPPDTSELHDLSKSSIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASG 480

Query: 537  ETNVVSCLSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLS 596
            ET VVSCLSSGSS+EP+SH RAKS LK A IHLGKV+V GAST NK LSAHGL KPDVLS
Sbjct: 481  ETKVVSCLSSGSSIEPVSHYRAKSALKLANIHLGKVDVIGASTMNKALSAHGLVKPDVLS 540

Query: 597  LSS-----RGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI--------- 656
            LS+      GDVSR    +   L   +T          C   +   L + +         
Sbjct: 541  LSNPVLEHLGDVSRTHHEIKDQLAGDSTKSYLDETNECCSQGMQNALDDQLRGAKDCNEQ 600

Query: 657  -------------LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQEC 716
                          ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E 
Sbjct: 601  SSEQVEVANSSYCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGIFED-VSNDSNGWES 660

Query: 717  YAFDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHID 776
             +FD      QDE  ET KMR+L T + E SDVDH ISNE               SMHID
Sbjct: 661  CSFD------QDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHID 720

Query: 777  DEKPTVPMSNNTS----GFSLTSQHDYASK--NDILAEGSNGVSDACLDSGPVVRNCNNH 836
            DE PT P+ NN S    G+SL SQ+++ +K  NDI     +   D           CNN 
Sbjct: 721  DENPTGPILNNQSLQGNGYSLASQNEFLTKESNDICENKVDSDYDLSSIPHSTADACNNC 780

Query: 837  TDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQLYEA 896
            TD+MVDI SD++QN TS ETER Q+  GDV IA NAD AET LI+RD  SSDTENQLYEA
Sbjct: 781  TDEMVDIVSDMQQNHTSFETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQLYEA 840

Query: 897  QMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSNDDLL 956
             + I SEHVQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS DDLL
Sbjct: 841  HIRIESEHVQNEDKQNSLVQSSVND------------CCIDLVDVSPKNNQGKCSIDDLL 900

Query: 957  HTSNSE-----------------------------GYKEET----ITDSLIASSDCLRRL 1016
            H SNSE                                EET      D+++ S +    L
Sbjct: 901  HRSNSEEIISVDNYDVCTSEGLSNCNQMASPKDNNSIHEETHDTRTGDNILESRELEASL 960

Query: 1017 GISKCSTAISSECEKVRSGEGAYETMSIEITSEARTTCNDLAFC---------------- 1076
              S+CSTA SSE +K  SGEG  ETMS +ITSE+R TCND +FC                
Sbjct: 961  RSSRCSTAKSSEDDK--SGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSSIPNDDKL 1020

Query: 1077 ----------------------------------------------WLTKELDSSVPNDH 1086
                                                            TK+L SSVPND 
Sbjct: 1021 SSANVQQYAGTKELENHKSPEMNEMCSTYNDNAQSEATTCNDSSFRSPTKDLGSSVPNDD 1080

BLAST of Sgr023386 vs. ExPASy TrEMBL
Match: A0A6J1CYM8 (uncharacterized protein LOC111015485 OS=Momordica charantia OX=3673 GN=LOC111015485 PE=4 SV=1)

HSP 1 Score: 1132.9 bits (2929), Expect = 0.0e+00
Identity = 684/1117 (61.24%), Postives = 784/1117 (70.19%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISL+EVAGEDD LL  IPE++LLNLDKDTD +TAGNSGFFLCSPLLTDRS+GTVAG
Sbjct: 1    MESEISLLEVAGEDDFLLQQIPEDDLLNLDKDTDRITAGNSGFFLCSPLLTDRSNGTVAG 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS ASSTD    C+  DKEN+N NNLEVPKLSIM QQMK+KKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASSTD----CT--DKENINVNNLEVPKLSIMSQQMKKKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG TSTSCGEALAAIDEE+PAMPGGGC +D+SS DK F D S STP
Sbjct: 121  TEEGVLDSMELSMITGSTSTSCGEALAAIDEEIPAMPGGGCYNDLSSQDKLFTDASISTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
              DRKNGR LLPKRGS+TKDNVKSKELSTK+ NRS SKRGSCPRPVASS    PTTS GT
Sbjct: 181  SADRKNGRCLLPKRGSTTKDNVKSKELSTKNSNRSVSKRGSCPRPVASSSTKRPTTSKGT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTRSTTIRASDAKSNQVAQRGVG--NNPKQTTLKSS 356
            KTV K+ERISR+ V KRDP VTS T R+ T  AS+AK+NQVAQRGVG  NNPK+TTLK S
Sbjct: 241  KTVNKEERISRVLVPKRDPAVTSWTPRNATTHASNAKNNQVAQRGVGVVNNPKRTTLKGS 300

Query: 357  SINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLKAATNS 416
            +IN KSAL+KDVNA+K+LKAKTSIQQPRRKLASPV+KM+SSHL NESTDSNE LKAA+ S
Sbjct: 301  AINTKSALHKDVNANKSLKAKTSIQQPRRKLASPVIKMSSSHLQNESTDSNEELKAASKS 360

Query: 417  LISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQKKVSS 476
            LISK +P NDDGTRKVSAS+TQNA P GG ML+QTQMPKPSGLRMPSPSMGFFGQKKVSS
Sbjct: 361  LISKFIPFNDDGTRKVSASITQNAPPTGGSMLSQTQMPKPSGLRMPSPSMGFFGQKKVSS 420

Query: 477  FQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGETNVVSC 536
            FQ+L PATSE H LSKS +PN+RTAGPSN  CQLA  L P NFVKAS+ EASGETNVVSC
Sbjct: 421  FQNLAPATSETHDLSKSYIPNVRTAGPSNYTCQLA-ALAPTNFVKASNGEASGETNVVSC 480

Query: 537  LSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLSSR--- 596
             SSG SV+P+SH RAKS+ +AA IHLGKVNV GAST N DLSAHGLEKPD+LSLS+    
Sbjct: 481  FSSGISVQPVSHYRAKSISEAANIHLGKVNVVGASTMNNDLSAHGLEKPDLLSLSNPVLQ 540

Query: 597  --GDVSRYMMRLTTNLQN-----------ANTVMCHL----------------------- 656
              GDVSR    +   L               +   HL                       
Sbjct: 541  NFGDVSRIHDEIKVQLAECKPRHLSFNNFGESTKSHLGEKNGLGSQGLPRALSNGSQDFN 600

Query: 657  ---TILLTLPN--HILVKRTTWAHKRLGVGTCNSLKRSRTSKDFEH-GRFEENVGNDSNG 716
               +  +  PN    +++R +   +R G+G+CNSLKRSR+S D EH G FEEN+GN S  
Sbjct: 601  EPSSEQVEFPNSPKCILERISSDSQRSGIGSCNSLKRSRSSIDLEHGGSFEENIGNGS-- 660

Query: 717  QECYAFDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SM 776
                AF+KD+NEGQDE   T +MR+L T+E E S +DHCISNE               SM
Sbjct: 661  ---CAFEKDVNEGQDETLGTRRMRILRTKEVESSGIDHCISNECKNTIQSTSAWSNSDSM 720

Query: 777  HIDDEKPTVPMSNNTS----GFSLTSQHDYAS-KNDILAEGSNGV--------------- 836
            HIDDEKPT+PMSN+ S    G SLTS HD+ S +N+ILA  SNGV               
Sbjct: 721  HIDDEKPTIPMSNSKSLQREGSSLTSHHDFNSHENEILAGVSNGVGENNLKSEIDLCLIQ 780

Query: 837  ---SDACLDSGPVVRNCNNHTDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAE 896
               +DACLD GPVVRNCN+HTD+M DI S+IEQN+T LE +RN D +GDVAIA N DAAE
Sbjct: 781  HSTADACLDIGPVVRNCNDHTDEMADISSNIEQNNTDLEMKRNLDDYGDVAIARNTDAAE 840

Query: 897  TFLITRDLCSSDTENQLYEAQMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCI 956
            T  +  DLC+SDTENQLYEAQ+ IGSE V NED QNSHV SSVN+FN+LP    LQN C 
Sbjct: 841  TLPLNPDLCTSDTENQLYEAQIQIGSEQVPNEDPQNSHVQSSVNDFNQLP---GLQNRCT 900

Query: 957  DLVEVSQNNNQGKCSNDDLLHTSNSEGYKEETITDSLIASSDCLRRLGISKCSTAISSEC 1016
            DLV+VSQNN+QGKCSNDD LHTSNSE YK+E IT+ +I  ++            A  +  
Sbjct: 901  DLVDVSQNNHQGKCSNDD-LHTSNSEEYKDEIITNGVIDYTN----------QVASHNNL 960

Query: 1017 EKVRSGEGAYETMSIEITSEARTTCNDLAFCWLTKELDSSVPNDHILSRENVQQCMETKE 1076
            E        ++ +S    S   +T N                     S E+ + C     
Sbjct: 961  E--------FQEISASARSSKHSTAN---------------------SSEHTETCS---- 1020

Query: 1077 LENHKSPQMNGNILCQNENELNSEWSIYQALKRAKKVRGCQKQNALGIKTSTNAVPFSEE 1086
                          C +           Q++++  K    +KQ   G +T TNAVPFSEE
Sbjct: 1021 -------------TCDDN---------AQSMQQLTKSEDVRKQ---GGRTETNAVPFSEE 1033

BLAST of Sgr023386 vs. ExPASy TrEMBL
Match: A0A6J1JHG3 (uncharacterized protein LOC111484416 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111484416 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 694/1210 (57.36%), Postives = 798/1210 (65.95%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE++LL+L+++ DG TA NSGFFLCSPLLT+RS+GT++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDDLLDLEREMDGATARNSGFFLCSPLLTNRSNGTISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L A+DEE+PA+P GG  +D+S  DK FK+TSTSTP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAVDEEIPAVPSGGSYNDLSFQDKLFKNTSTSTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRG------VGNNPKQT 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQRG      V +NPK T
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQRGHQVAQRVVSNPKMT 300

Query: 357  TLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLK 416
            T   SS+NA  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEGLK
Sbjct: 301  TPNGSSMNAIRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEGLK 360

Query: 417  AATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQ 476
            A  NSLISK LP NDDGT+KVSAS+TQNA  +G  MLN TQMPKPSGLRMPSPSMGFFGQ
Sbjct: 361  ARENSLISKPLPSNDDGTKKVSASITQNAPHNGRSMLNPTQMPKPSGLRMPSPSMGFFGQ 420

Query: 477  KKVSSFQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGET 536
            KKVSSFQS+PP TSE+H LSKSN+PN+R  GPSNPICQLA TLVPRN VKA+  EASGET
Sbjct: 421  KKVSSFQSVPPDTSELHDLSKSNIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASGET 480

Query: 537  NVVSCLSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLS 596
             VVSCLSSGSS+EP+SHDRAKS LK A IHLGKV+V GAST  K LSAHGL KPDVLSLS
Sbjct: 481  KVVSCLSSGSSIEPLSHDRAKSALKVANIHLGKVDVIGASTMKKALSAHGLVKPDVLSLS 540

Query: 597  -----SRGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI----------- 656
                 + GDVSR    +   L   +T          C   +   L + +           
Sbjct: 541  NPVLENLGDVSRKHHEIKDQLAGDSTKSYLDETNESCSQGMQNALDDQLRGAKDCNEQSS 600

Query: 657  -----------LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQECYA 716
                        ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E  +
Sbjct: 601  EQVEVANSFNCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGTFED-VSNDSNGWESCS 660

Query: 717  FDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHIDDE 776
            FD       DE  ET KMR+L T + E SDVDH ISNE               SMHIDDE
Sbjct: 661  FD------LDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHIDDE 720

Query: 777  KPTVPMSNNTS----GFSLTSQHDYASKNDILAEGSNGVSDACLDSGPVVRN-------- 836
             PT P+ NN S    G SL      AS+N++L + SN + +  +DS   + +        
Sbjct: 721  NPTGPILNNKSLQGNGCSL------ASQNEVLTKESNDICENKVDSDYELSSIPHSTADA 780

Query: 837  CNNHTDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQ 896
            CN +TD++VDI SD++QN TSLETER Q+  GDV IA NAD AET LI+RD  SSDTENQ
Sbjct: 781  CNKYTDEIVDIVSDMQQNHTSLETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQ 840

Query: 897  LYEAQMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSN 956
            LYEA + I SEHVQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS 
Sbjct: 841  LYEAHIRIESEHVQNEDKQNSFVQSSVND------------CCIDLVDVSPKNNQGKCSI 900

Query: 957  DDLLHTSNSE-------------------------------GYKEET----ITDSLIASS 1016
            D LLH S+SE                                 +EET      D+++ S 
Sbjct: 901  DHLLHRSSSEEIITGSVDNSDVCTSEGLSNCNQMASPKDNSSIQEETHDTRTGDNILESQ 960

Query: 1017 DCLRRLGISKCSTAISSECEK-VRSGEGAYETMSIEITSEARTTCND------------- 1076
            +    +  S CSTA SSE +K + SGEG  ETMS +ITSE+R TCND             
Sbjct: 961  EIEASISSSSCSTAKSSEYDKIILSGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSS 1020

Query: 1077 -------------------------------------------------LAFCWLTKELD 1086
                                                              +FC  TK+L 
Sbjct: 1021 IPIDDTLSSENVQQYVGTKELDNHKSPEMNEMCSTYNDNAQSEATTCNVSSFCSPTKDLG 1080

BLAST of Sgr023386 vs. ExPASy TrEMBL
Match: A0A6J1JGY6 (uncharacterized protein LOC111484416 isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111484416 PE=4 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 694/1209 (57.40%), Postives = 796/1209 (65.84%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE++LL+L+++ DG TA NSGFFLCSPLLT+RS+GT++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDDLLDLEREMDGATARNSGFFLCSPLLTNRSNGTISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L A+DEE+PA+P GG  +D+S  DK FK+TSTSTP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAVDEEIPAVPSGGSYNDLSFQDKLFKNTSTSTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRG------VGNNPKQT 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQRG      V +NPK T
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQRGHQVAQRVVSNPKMT 300

Query: 357  TLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLK 416
            T   SS+NA  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEGLK
Sbjct: 301  TPNGSSMNAIRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEGLK 360

Query: 417  AATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQ 476
            A  NSLISK LP NDDGT+KVSAS+TQNA  +G  MLN TQMPKPSGLRMPSPSMGFFGQ
Sbjct: 361  ARENSLISKPLPSNDDGTKKVSASITQNAPHNGRSMLNPTQMPKPSGLRMPSPSMGFFGQ 420

Query: 477  KKVSSFQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGET 536
            KKVSSFQS+PP TSE+H LSKSN+PN+R  GPSNPICQLA TLVPRN VKA+  EASGET
Sbjct: 421  KKVSSFQSVPPDTSELHDLSKSNIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASGET 480

Query: 537  NVVSCLSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLS 596
             VVSCLSSGSS+EP+SHDRAKS LK A IHLGKV+V GAST  K LSAHGL KPDVLSLS
Sbjct: 481  KVVSCLSSGSSIEPLSHDRAKSALKVANIHLGKVDVIGASTMKKALSAHGLVKPDVLSLS 540

Query: 597  -----SRGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI----------- 656
                 + GDVSR    +   L   +T          C   +   L + +           
Sbjct: 541  NPVLENLGDVSRKHHEIKDQLAGDSTKSYLDETNESCSQGMQNALDDQLRGAKDCNEQSS 600

Query: 657  -----------LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQECYA 716
                        ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E  +
Sbjct: 601  EQVEVANSFNCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGTFED-VSNDSNGWESCS 660

Query: 717  FDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHIDDE 776
            FD       DE  ET KMR+L T + E SDVDH ISNE               SMHIDDE
Sbjct: 661  FD------LDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHIDDE 720

Query: 777  KPTVPMSNNTS----GFSLTSQHDYASKNDILAEGSNGVSDACLDSGPVVRN-------- 836
             PT P+ NN S    G SL      AS+N++L + SN + +  +DS   + +        
Sbjct: 721  NPTGPILNNKSLQGNGCSL------ASQNEVLTKESNDICENKVDSDYELSSIPHSTADA 780

Query: 837  CNNHTDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQ 896
            CN +TD++VDI SD++QN TSLETER Q+  GDV IA NAD AET LI+RD  SSDTENQ
Sbjct: 781  CNKYTDEIVDIVSDMQQNHTSLETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQ 840

Query: 897  LYEAQMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSN 956
            LYEA + I SEHVQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS 
Sbjct: 841  LYEAHIRIESEHVQNEDKQNSFVQSSVND------------CCIDLVDVSPKNNQGKCSI 900

Query: 957  DDLLHTSNSE-------------------------------GYKEET----ITDSLIASS 1016
            D LLH S+SE                                 +EET      D+++ S 
Sbjct: 901  DHLLHRSSSEEIITGSVDNSDVCTSEGLSNCNQMASPKDNSSIQEETHDTRTGDNILESQ 960

Query: 1017 DCLRRLGISKCSTAISSECEK-VRSGEGAYETMSIEITSEARTTCND------------- 1076
            +    +  S CSTA SSE +K + SGEG  ETMS +ITSE+R TCND             
Sbjct: 961  EIEASISSSSCSTAKSSEYDKIILSGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSS 1020

Query: 1077 -------------------------------------------------LAFCWLTKELD 1086
                                                              +FC  TK+L 
Sbjct: 1021 IPIDDTLSSENVQQYVGTKELDNHKSPEMNEMCSTYNDNAQSEATTCNVSSFCSPTKDLG 1080

BLAST of Sgr023386 vs. ExPASy TrEMBL
Match: A0A6J1FRC2 (uncharacterized protein LOC111448106 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111448106 PE=4 SV=1)

HSP 1 Score: 1089.3 bits (2816), Expect = 0.0e+00
Identity = 703/1265 (55.57%), Postives = 793/1265 (62.69%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE+ LL+L+++ DG TA NSGFFLCSPLLT+RS G ++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDGLLDLEREMDGATARNSGFFLCSPLLTNRSIGAISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L AIDEE+PAMPGG   +D+S  DK FKDTST TP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAIDEEIPAMPGGSSYNDLSFQDKLFKDTSTGTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRGVGNNPKQTTLKSSS 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQR V +NPK +T KSSS
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQR-VVSNPKMSTSKSSS 300

Query: 357  INAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEGLKAATNSL 416
            +N+  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEGLKA  NSL
Sbjct: 301  MNSLRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEGLKAGANSL 360

Query: 417  ISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFFGQKKVSSF 476
            ISK LP NDDGT+K SAS+TQNA PDG  MLN TQMPKPSGLRMPSPSMGFFGQKKVSSF
Sbjct: 361  ISKPLPSNDDGTKKASASITQNAPPDGRSMLNPTQMPKPSGLRMPSPSMGFFGQKKVSSF 420

Query: 477  QSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASGETNVVSCL 536
            QS+PP TSE+H LSKS++PN+R  GPSNPICQLA TLVPRN VKA+  EASGET VVSCL
Sbjct: 421  QSVPPDTSELHDLSKSSIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASGETKVVSCL 480

Query: 537  SSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLSLSS----- 596
            SSGSS+EP+SH RAKS LK A IHLGKV+V GAST NK LSAHGL KPDVLSLS+     
Sbjct: 481  SSGSSIEPVSHYRAKSALKLANIHLGKVDVIGASTMNKALSAHGLVKPDVLSLSNPVLEH 540

Query: 597  RGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI----------------- 656
             GDVSR    +   L   +T          C   +   L + +                 
Sbjct: 541  LGDVSRTHHEIKDQLAGDSTKSYLDETNECCSQGMQNALDDQLRGAKDCNEQSSEQVEVA 600

Query: 657  -----LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQECYAFDKDMN 716
                  ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E  +FD    
Sbjct: 601  NSSYCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGIFED-VSNDSNGWESCSFD---- 660

Query: 717  EGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHIDDEKPTVPM 776
              QDE  ET KMR+L T + E SDVDH ISNE               SMHIDDE PT P+
Sbjct: 661  --QDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHIDDENPTGPI 720

Query: 777  SNNTS----GFSLTSQHDYASK--NDILAEGSNGVSDACLDSGPVVRNCNNHTDKMVDIR 836
             NN S    G+SL SQ+++ +K  NDI     +   D           CNN TD+MVDI 
Sbjct: 721  LNNQSLQGNGYSLASQNEFLTKESNDICENKVDSDYDLSSIPHSTADACNNCTDEMVDIV 780

Query: 837  SDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQLYEAQMCIGSEH 896
            SD++QN TS ETER Q+  GDV IA NAD AET LI+RD  SSDTENQLYEA + I SEH
Sbjct: 781  SDMQQNHTSFETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQLYEAHIRIESEH 840

Query: 897  VQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSNDDLLHTSNSE-- 956
            VQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS DDLLH SNSE  
Sbjct: 841  VQNEDKQNSLVQSSVND------------CCIDLVDVSPKNNQGKCSIDDLLHRSNSEEI 900

Query: 957  ---------------------------GYKEET----ITDSLIASSDCLRRLGISKCSTA 1016
                                          EET      D+++ S +    L  S+CSTA
Sbjct: 901  ISVDNYDVCTSEGLSNCNQMASPKDNNSIHEETHDTRTGDNILESRELEASLRSSRCSTA 960

Query: 1017 ISSECEKVRSGEGAYETMSIEITSEARTTCNDLAFC------------------------ 1076
             SSE +K  SGEG  ETMS +ITSE+R TCND +FC                        
Sbjct: 961  KSSEDDK--SGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSSIPNDDKLSSANVQQY 1020

Query: 1077 --------------------------------------WLTKELDSSVPNDHILSRENVQ 1086
                                                    TK+L SSVPND ILSRENVQ
Sbjct: 1021 AGTKELENHKSPEMNEMCSTYNDNAQSEATTCNDSSFRSPTKDLGSSVPNDDILSRENVQ 1080

BLAST of Sgr023386 vs. ExPASy TrEMBL
Match: A0A6J1FTV2 (uncharacterized protein LOC111448106 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448106 PE=4 SV=1)

HSP 1 Score: 1088.9 bits (2815), Expect = 0.0e+00
Identity = 704/1273 (55.30%), Postives = 794/1273 (62.37%), Query Frame = 0

Query: 57   MESDISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAG 116
            MES+ISLIEVAGEDDSLL  IPE+ LL+L+++ DG TA NSGFFLCSPLLT+RS G ++ 
Sbjct: 1    MESNISLIEVAGEDDSLLQQIPEDGLLDLEREMDGATARNSGFFLCSPLLTNRSIGAISS 60

Query: 117  SSAASSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFF 176
            SS AS+ D      + DKEN+NAN++E P LSIMPQQMKRKKK GGYNLRKSLAWNKAFF
Sbjct: 61   SSTASAAD------HTDKENINANDIEGPNLSIMPQQMKRKKKAGGYNLRKSLAWNKAFF 120

Query: 177  TEEGVLDSVELSMITGRTSTSCGEALAAIDEELPAMPGGGCIDDISSHDKFFKDTSTSTP 236
            TEEGVLDS+ELSMITG T+T     L AIDEE+PAMPGG   +D+S  DK FKDTST TP
Sbjct: 121  TEEGVLDSMELSMITGSTNT-----LGAIDEEIPAMPGGSSYNDLSFQDKLFKDTSTGTP 180

Query: 237  GGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPVASS----PTTSNGT 296
             GDRKNGR LLPKRGSSTKDNVK KELS KD+NRSGSKRGSCPRPVASS    PTTSN T
Sbjct: 181  IGDRKNGRCLLPKRGSSTKDNVKLKELSAKDLNRSGSKRGSCPRPVASSSVKRPTTSNAT 240

Query: 297  KTVIKDERISRIPVSKRDPTVTSRTTR-STTIRASDAKSNQVAQRG--------VGNNPK 356
            KT+ K+ER SRIPV KRD TV  R  R + TIRASDAKSNQVAQRG        V +NPK
Sbjct: 241  KTMNKEERTSRIPVPKRDSTVIPRAPRNAATIRASDAKSNQVAQRGNHCKIAQTVVSNPK 300

Query: 357  QTTLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMNSSHLHNESTDSNEG 416
             +T KSSS+N+  ALNKDVNASK+LKAK SIQQP  KLA+PVLK+NSS   +ES D NEG
Sbjct: 301  MSTSKSSSMNSLRALNKDVNASKSLKAKNSIQQP-GKLANPVLKVNSSRCQHESVDPNEG 360

Query: 417  LKAATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQTQMPKPSGLRMPSPSMGFF 476
            LKA  NSLISK LP NDDGT+K SAS+TQNA PDG  MLN TQMPKPSGLRMPSPSMGFF
Sbjct: 361  LKAGANSLISKPLPSNDDGTKKASASITQNAPPDGRSMLNPTQMPKPSGLRMPSPSMGFF 420

Query: 477  GQKKVSSFQSLPPATSEMHSLSKSNLPNLRTAGPSNPICQLATTLVPRNFVKASSREASG 536
            GQKKVSSFQS+PP TSE+H LSKS++PN+R  GPSNPICQLA TLVPRN VKA+  EASG
Sbjct: 421  GQKKVSSFQSVPPDTSELHDLSKSSIPNVRIRGPSNPICQLA-TLVPRNVVKANHGEASG 480

Query: 537  ETNVVSCLSSGSSVEPISHDRAKSVLKAAEIHLGKVNVGGASTTNKDLSAHGLEKPDVLS 596
            ET VVSCLSSGSS+EP+SH RAKS LK A IHLGKV+V GAST NK LSAHGL KPDVLS
Sbjct: 481  ETKVVSCLSSGSSIEPVSHYRAKSALKLANIHLGKVDVIGASTMNKALSAHGLVKPDVLS 540

Query: 597  LSS-----RGDVSRYMMRLTTNLQNANT--------VMCHLTILLTLPNHI--------- 656
            LS+      GDVSR    +   L   +T          C   +   L + +         
Sbjct: 541  LSNPVLEHLGDVSRTHHEIKDQLAGDSTKSYLDETNECCSQGMQNALDDQLRGAKDCNEQ 600

Query: 657  -------------LVKRTTWAHKRLGVGTCNSLKRSRTSKDFEHGRFEENVGNDSNGQEC 716
                          ++RT+  H+R+G+GTCNSLKRSRTS +F+HG FE+ V NDSNG E 
Sbjct: 601  SSEQVEVANSSYCKIERTSSDHQRVGIGTCNSLKRSRTSIEFDHGIFED-VSNDSNGWES 660

Query: 717  YAFDKDMNEGQDEASETHKMRLLGTEEPEGSDVDHCISNEY--------------SMHID 776
             +FD      QDE  ET KMR+L T + E SDVDH ISNE               SMHID
Sbjct: 661  CSFD------QDEDLETRKMRILRTRKAEPSDVDHFISNECNNTMQTATELSNSDSMHID 720

Query: 777  DEKPTVPMSNNTS----GFSLTSQHDYASK--NDILAEGSNGVSDACLDSGPVVRNCNNH 836
            DE PT P+ NN S    G+SL SQ+++ +K  NDI     +   D           CNN 
Sbjct: 721  DENPTGPILNNQSLQGNGYSLASQNEFLTKESNDICENKVDSDYDLSSIPHSTADACNNC 780

Query: 837  TDKMVDIRSDIEQNDTSLETERNQDGHGDVAIAHNADAAETFLITRDLCSSDTENQLYEA 896
            TD+MVDI SD++QN TS ETER Q+  GDV IA NAD AET LI+RD  SSDTENQLYEA
Sbjct: 781  TDEMVDIVSDMQQNHTSFETERYQNDRGDVEIACNADVAETLLISRDFHSSDTENQLYEA 840

Query: 897  QMCIGSEHVQNEDKQNSHVPSSVNEFNRLPEFAELQNCCIDLVEVSQNNNQGKCSNDDLL 956
             + I SEHVQNEDKQNS V SSVN+            CCIDLV+VS  NNQGKCS DDLL
Sbjct: 841  HIRIESEHVQNEDKQNSLVQSSVND------------CCIDLVDVSPKNNQGKCSIDDLL 900

Query: 957  HTSNSE-----------------------------GYKEET----ITDSLIASSDCLRRL 1016
            H SNSE                                EET      D+++ S +    L
Sbjct: 901  HRSNSEEIISVDNYDVCTSEGLSNCNQMASPKDNNSIHEETHDTRTGDNILESRELEASL 960

Query: 1017 GISKCSTAISSECEKVRSGEGAYETMSIEITSEARTTCNDLAFC---------------- 1076
              S+CSTA SSE +K  SGEG  ETMS +ITSE+R TCND +FC                
Sbjct: 961  RSSRCSTAKSSEDDK--SGEGTSETMS-KITSESRMTCNDQSFCSPTKDLGSSIPNDDKL 1020

Query: 1077 ----------------------------------------------WLTKELDSSVPNDH 1086
                                                            TK+L SSVPND 
Sbjct: 1021 SSANVQQYAGTKELENHKSPEMNEMCSTYNDNAQSEATTCNDSSFRSPTKDLGSSVPNDD 1080

BLAST of Sgr023386 vs. TAIR 10
Match: AT5G60150.1 (unknown protein; Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 122.9 bits (307), Expect = 1.8e-27
Identity = 133/448 (29.69%), Postives = 205/448 (45.76%), Query Frame = 0

Query: 60  DISLIEVAGEDDSLLHPIPENELL-NLDKDTDGVTAGNSGFFLCSPLLTDRSSGTV---- 119
           D  L++++GEDD       +N LL N  K T+  ++    +  CSPL   RSS  V    
Sbjct: 2   DKDLLDISGEDDE------DNWLLKNTPKKTN--SSRGKSYLKCSPLQIPRSSRIVPTRP 61

Query: 120 ----AGSSAASSTDIVCRCSYIDKENVNANN--LEVPKLSIMPQQMKRKKKGGGYNLRKS 179
                G    +S +    C+ +D ++V   N  +E+PKLS+  QQMK+KKK  G+NLRKS
Sbjct: 62  PFSPIGRVTGTSNNREQPCASVDTDSVGKENAKVELPKLSVERQQMKKKKKNAGFNLRKS 121

Query: 180 LAWNKAFFTEEGVLDSVELSMITGRTSTSCGEALAAIDEEL-PAMPGGGCIDDISS---- 239
           LAW++AF TEEGVLDS ELS ITG      G+ LAAI EE   +M    C  ++S     
Sbjct: 122 LAWDRAFSTEEGVLDSSELSKITGTACHLGGDRLAAIQEEYRESMSASKC--NVSPGLQA 181

Query: 240 -HDKFFKDTSTSTPGGDRKNGRYLLPKRGSSTKDNVKSKELSTKDVNRSGSKRGSCPRPV 299
             +  F D   ++   ++K    ++P            KELS                  
Sbjct: 182 LEENLFNDLPVNSKNREKKLVSGIMP------------KELS------------------ 241

Query: 300 ASSPTTSNGTKTVIKDERISRIPVSKRDPTVTSRTTRSTT---IRASDAKSNQV--AQRG 359
                             IS++P +K DP       + TT   I+A +++  Q+  +QR 
Sbjct: 242 ------------------ISKVPTTKSDPVTVGNNMKRTTQSPIKAKNSQPTQLKNSQRS 301

Query: 360 VGNNPKQTTLKSSSINAKSALNKDVNASKTLKAKTSIQQPRRKLASPVLKMN--SSHLHN 419
           +G+        S+    KS+L     ASK+   K S++Q RR + S   ++   S   H+
Sbjct: 302 LGSESFSKNTSSTKSKTKSSL-----ASKSSIPKPSLKQARRNVISKSSEIPTVSYSQHS 361

Query: 420 ESTDSNEG-LKAATNSLISKVLPLNDDGTRKVSASVTQNALPDGGRMLNQ-TQMPKPSGL 479
               SN G + A+  +++     + D     +  S+ Q++    G   +  +++ KPSGL
Sbjct: 362 VVAKSNVGPMTASDVAMLGHASVIPDSNVITLGTSLAQSSCNKAGSTQSAVSRLGKPSGL 386

Query: 480 RMPSPSMGFFGQKKVSSFQSLPPATSEM 482
           R P PS+G+F Q      QS     S++
Sbjct: 422 RAPKPSIGYFSQSDSQPSQSAGDKHSQL 386

HSP 2 Score: 80.1 bits (196), Expect = 1.3e-14
Identity = 37/45 (82.22%), Postives = 42/45 (93.33%), Query Frame = 0

Query: 1041 NAVPFSEEWLAAIEAAGEEILTMKTGAVQNSPPDKSQPEPGPWSP 1086
            NAVPFS+EWLAAIEAAGEEILT+K+G VQ+SP +KS PEPGPWSP
Sbjct: 1125 NAVPFSDEWLAAIEAAGEEILTLKSGRVQHSPTEKSAPEPGPWSP 1169

BLAST of Sgr023386 vs. TAIR 10
Match: AT3G53320.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G37070.1); Has 11044 Blast hits to 5993 proteins in 551 species: Archae - 8; Bacteria - 1486; Metazoa - 4078; Fungi - 1814; Plants - 348; Viruses - 112; Other Eukaryotes - 3198 (source: NCBI BLink). )

HSP 1 Score: 45.1 bits (105), Expect = 4.7e-04
Identity = 127/516 (24.61%), Postives = 202/516 (39.15%), Query Frame = 0

Query: 61  ISLIEVAGEDDSLLHPIPENELLNLDKDTDGVTAGNSGFFLCSPLLTDRSSGTVAGSSAA 120
           + LI+VA EDDSLL     +E    DKD   +       F+      D            
Sbjct: 16  LGLIDVAVEDDSLLF----SEFSETDKDDKCLKEDKDLNFMRDTQYCD------------ 75

Query: 121 SSTDIVCRCSYIDKENVNANNLEVPKLSIMPQQMKRKKKGGGYNLRKSLAWNKAFFTEEG 180
              D +   S  +KE V       P  S  P+++ +K   G YNLRKSLAW+  FFT  G
Sbjct: 76  ---DEILASSVEEKEEVLQ-----PHESPEPEKVMKK---GKYNLRKSLAWDNEFFTSAG 135

Query: 181 VLDSVELSMITGRTSTSCGEALAAIDEEL------------------------------- 240
           VL+  ELS +      S  +AL  I E++                               
Sbjct: 136 VLEPEELSSMMESNHKSGKKALPTILEDINRSTESISTFQSDCTVENSQEFVLFEDVRAS 195

Query: 241 ---------PAMPGGGCIDDISSHDKFFKDTS-----TSTPGGDRKNGRYLLPKR----G 300
                     A PG   +  + + D     TS     T+T G  +  G    P R    G
Sbjct: 196 IQRSAKTSDVATPGKSNV--LRATDVAISPTSSTVDVTATQGKTKSKGSPRNPSRVQGPG 255

Query: 301 SSTKDNVKSKELSTKDVNRSGSKRG-SCPRPVASSPTTSNGTK-TVIKDERISRIPVSKR 360
            +TK  V ++ LST   + S    G S  RP++++ T  +    +  + E+ S++P  K 
Sbjct: 256 KATKQPVATRGLST---SISKPPNGLSKVRPLSTTSTNRSSLDISKTQQEKNSKLPAGKE 315

Query: 361 --DPTVT-SRTTR----------STTIRASDAKSNQV------------AQRGVGNNPKQ 420
              P ++ SR  +           ++ R+SDA  N++            A     + P  
Sbjct: 316 PLGPRISMSRRAKPVLPKPGVPFKSSSRSSDASKNEMTSSCSSLESCASASSSASHKPSI 375

Query: 421 TTLK-----SSSINAKSALNKDVNASKTLKAKTSIQQPRR----KLASPVLKMNS-SHLH 474
            ++K     SS ++++   N+  +     + +   QQ  +    KL+S V    S S   
Sbjct: 376 DSIKKKNDSSSRLSSQPLANRSTSRGIMGQPRIPPQQTNKTSKPKLSSSVPTAGSISDYS 435