Sgr023368 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023368 vs. NCBI nr
Match: XP_023511975.1 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 489.2 bits (1258), Expect = 3.0e-134
Identity = 268/370 (72.43%), Postives = 289/370 (78.11%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP NDD NT++ VPNIPVEIVS+EEMALIEAALAAAV TAR     SS++AI
Sbjct: 22  MADSSSGLPPFNDDDNTHKGVPNIPVEIVSDEEMALIEAALAAAVTTAR-----SSSSAI 81

Query: 61  HSPSCSSQNLFNT----------------------------STQKRIKLNESLLHRFRRK 120
           H PSCSSQNLFNT                            STQK+IK+NESLL RFRR 
Sbjct: 82  HPPSCSSQNLFNTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRN 141

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KGLAVTDIT TEWCEKQME  LL G RK TKAM+AGIARHAMLEAEVV+KVKVQVKSAED
Sbjct: 142 KGLAVTDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAED 201

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF FGVNQLLFEGLTREL           MGLVEGVWMVGVIDEI+MLEI + 
Sbjct: 202 NWALKLLNFTFGVNQLLFEGLTREL---------PVMGLVEGVWMVGVIDEIQMLEIGTC 261

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           K+PML+DTKTR   TIP EPQQRNGKLQLMCYK+LLD LI DGFS+RQFFDFFSLNP+S 
Sbjct: 262 KVPMLIDTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSI 321

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI +ST+S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+I EDEFAY
Sbjct: 322 LSEEIADSTASSGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIGEDEFAY 377

BLAST of Sgr023368 vs. NCBI nr
Match: XP_022985622.1 (exonuclease V, chloroplastic [Cucurbita maxima] >XP_022985623.1 exonuclease V, chloroplastic [Cucurbita maxima] >XP_022985624.1 exonuclease V, chloroplastic [Cucurbita maxima])

HSP 1 Score: 488.0 bits (1255), Expect = 6.7e-134
Identity = 267/370 (72.16%), Postives = 289/370 (78.11%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP NDD NT++ VPNIPVEIVS+EEMALIEAALAAAV TAR     SS++AI
Sbjct: 22  MADSSSGLPPYNDDDNTHKGVPNIPVEIVSDEEMALIEAALAAAVTTAR-----SSSSAI 81

Query: 61  HSPSCSSQNLFNT----------------------------STQKRIKLNESLLHRFRRK 120
           H PSCSSQNLFNT                            STQK+IK+NESLL RFRR 
Sbjct: 82  HPPSCSSQNLFNTRSIHSITLLSKRGLPSSQLDVEDLCKSSSTQKKIKVNESLLQRFRRN 141

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KGLAVTDIT TEWCEKQME  LL G RK TKAM+AGIARHAMLEAEVV+KVKVQVKSAED
Sbjct: 142 KGLAVTDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAED 201

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF FGVNQLLFEGLTREL           MGLVEGVWMVGVIDEI+MLEI + 
Sbjct: 202 NWALKLLNFTFGVNQLLFEGLTREL---------PVMGLVEGVWMVGVIDEIQMLEIGTC 261

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           K+PML+DTKTR   TIP EPQQRNGKLQLMCYK+LLD LI DGFS+RQFFDFFSLNP+S 
Sbjct: 262 KVPMLIDTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSI 321

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI +S++S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+I EDEFAY
Sbjct: 322 LSEEIADSSASFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIREDEFAY 377

BLAST of Sgr023368 vs. NCBI nr
Match: XP_022943527.1 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 487.3 bits (1253), Expect = 1.1e-133
Identity = 267/370 (72.16%), Postives = 288/370 (77.84%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP NDD NT++ VPNIPVEIVS+EEMALIEAALAAAV TAR     SS++AI
Sbjct: 22  MADSSSGLPPFNDDDNTHKDVPNIPVEIVSDEEMALIEAALAAAVTTAR-----SSSSAI 81

Query: 61  HSPSCSSQNLFNT----------------------------STQKRIKLNESLLHRFRRK 120
           H PSCSSQNLFNT                            STQK+IK+NESLL RFRR 
Sbjct: 82  HPPSCSSQNLFNTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRT 141

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KGLAVTDIT TEWCEKQME  LL G RK TKAM+AGIARHAMLE+EVV+KVKVQVKSAED
Sbjct: 142 KGLAVTDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLESEVVKKVKVQVKSAED 201

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF FGVNQLLFEGLTREL           MGLVEGVWMVGVIDEI+MLEI + 
Sbjct: 202 NWALKLLNFTFGVNQLLFEGLTREL---------PVMGLVEGVWMVGVIDEIQMLEIGTC 261

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           K+PML+DTKTR   TIP EPQQRNGKLQLMCYK+LLD LI DGFS+RQFFDFFSLNP+S 
Sbjct: 262 KVPMLIDTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSI 321

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI +ST S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+I EDEFAY
Sbjct: 322 LSEEIADSTVSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIGEDEFAY 377

BLAST of Sgr023368 vs. NCBI nr
Match: KAG6570766.1 (Exonuclease V, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.3 bits (1253), Expect = 1.1e-133
Identity = 266/370 (71.89%), Postives = 288/370 (77.84%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP NDD NT++ +PNIPVEIVS+EEMALIEAALAAAV TAR     SS++AI
Sbjct: 1   MADSSSGLPPFNDDDNTHKDIPNIPVEIVSDEEMALIEAALAAAVNTAR-----SSSSAI 60

Query: 61  HSPSCSSQNLFNT----------------------------STQKRIKLNESLLHRFRRK 120
           H PSCSSQNLF+T                            STQK+IK+NESLL RFRR 
Sbjct: 61  HPPSCSSQNLFHTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRN 120

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KGLAVTDIT TEWCEKQME  LL G RK TKAM+AGIARHAMLEAEVV+KVKVQVKSAED
Sbjct: 121 KGLAVTDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAED 180

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF FGVNQLLFEGLTREL           MGLVEGVWMVGVIDEI+MLEI + 
Sbjct: 181 NWALKLLNFTFGVNQLLFEGLTREL---------PVMGLVEGVWMVGVIDEIQMLEIGTC 240

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           K+PML+DTKTR   TIP EPQQRNGKLQLMCYK+LLD LI DGFS+RQFFDFFSLNP+S 
Sbjct: 241 KVPMLIDTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSI 300

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI +ST S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+I EDEFAY
Sbjct: 301 LSEEIADSTVSFGFTAKTLDDVVRYYINCCSMLPPVHNQLLLRYESQKDQSIIGEDEFAY 356

BLAST of Sgr023368 vs. NCBI nr
Match: XP_022140310.1 (exonuclease V, chloroplastic isoform X1 [Momordica charantia])

HSP 1 Score: 483.0 bits (1242), Expect = 2.2e-132
Identity = 265/370 (71.62%), Postives = 291/370 (78.65%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M   GS  PP NDD +T+E VPNIPVEIVS+EEMALIEAALAA    ARSSIS SS++AI
Sbjct: 23  MADSGSGLPPFNDDESTHEGVPNIPVEIVSDEEMALIEAALAA----ARSSISSSSSSAI 82

Query: 61  HSPSCSSQNLFN----------------------------TSTQKRIKLNESLLHRFRRK 120
           +S S SS+ LFN                             STQKR+K+NESLLHRFRRK
Sbjct: 83  NS-SSSSKILFNARSIQSITLLSKRGLSAAHPDIEDLCKTASTQKRVKVNESLLHRFRRK 142

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KG AVTD TGTEWCEKQ  FSL  GSR+ TKA++AGIARHAMLEAEVV+K KVQVKSAED
Sbjct: 143 KGFAVTDFTGTEWCEKQKVFSLHSGSREKTKAVEAGIARHAMLEAEVVKKAKVQVKSAED 202

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
           RWALKLLNF FGVNQLLFEGLTREL           MGL+EGVWMVGVIDE+RMLE D+G
Sbjct: 203 RWALKLLNFTFGVNQLLFEGLTREL---------PVMGLIEGVWMVGVIDEVRMLETDTG 262

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           ++ MLVDTKTR+ DT+P EPQQRNGKLQLMCYKY+LDSLIPDGFSSRQFFDFFSLNPYS 
Sbjct: 263 RMLMLVDTKTRSRDTVPAEPQQRNGKLQLMCYKYILDSLIPDGFSSRQFFDFFSLNPYST 322

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI ES +S GFTAKTLDDV+R YTNCCSMLP  HN+LLLRYESQKDQS+IVE EF Y
Sbjct: 323 LSEEITESITSFGFTAKTLDDVIRYYTNCCSMLPSTHNKLLLRYESQKDQSIIVEVEFEY 378

BLAST of Sgr023368 vs. ExPASy Swiss-Prot
Match: Q9FKK6 (Exonuclease V, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At5g60370 PE=2 SV=1)

HSP 1 Score: 302.8 bits (774), Expect = 5.2e-81
Identity = 173/326 (53.07%), Postives = 223/326 (68.40%), Query Frame = 0

Query: 22  PNIPVEIVSEEEMALIEAALAAAVGTARS---SISCSSTTAIHSP-SCSSQNLFNTSTQK 81
           P IP+EIVSEEEMA+++AALAA+     S   S+S S  TA  SP +  S  LF+     
Sbjct: 40  PEIPIEIVSEEEMAILDAALAASRSILPSVIRSVSPSRITAGGSPKTIRSITLFSKRKLS 99

Query: 82  RIK-LNESLLHRFRRKKGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLE 141
               + ES LHRFRR + L VTD+TGTEWCEKQME  L FG RK  KAMK G ARH  LE
Sbjct: 100 ACSDIEESYLHRFRRNQALGVTDLTGTEWCEKQMENVLCFGRRKVNKAMKVGQARHLQLE 159

Query: 142 AEVVEKVKVQVKSAEDRWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVW 201
            EVV+KV+V+V+S ED+WALKLLN + GVNQ LFEG TRELL          +G V G W
Sbjct: 160 EEVVKKVRVKVESNEDKWALKLLNSIAGVNQFLFEGRTRELL---------LLGFVGGQW 219

Query: 202 MVGVIDEIR--MLEIDSGKIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPD 261
           +VG+IDE+R    E  S   P+L+DTKTR  DT+P EPQ+RNG+LQLM YK L D+++ +
Sbjct: 220 IVGIIDELRKAYAEESSDSGPILIDTKTRLRDTLPAEPQRRNGRLQLMLYKLLWDTIVKE 279

Query: 262 GFSSRQFFDFFSLNPYSPLSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLL 321
           GF +  FFD+FSLN    LS+++ +  ++ G  A+TL++++R Y N   MLP A+NQLLL
Sbjct: 280 GFPTGPFFDYFSLNRNYVLSQDVRDHIANAGIKAQTLEEIVRYYENTFQMLPIANNQLLL 339

Query: 322 RYESQKDQSVIVEDEFAYDHDWLKSQ 341
           +YE QKDQS+I E  F +DH+W+ S+
Sbjct: 340 KYEFQKDQSIIAEIRFNHDHEWVMSK 356

BLAST of Sgr023368 vs. ExPASy Swiss-Prot
Match: A2VDX7 (Exonuclease V OS=Bos taurus OX=9913 GN=EXO5 PE=2 SV=2)

HSP 1 Score: 93.2 bits (230), Expect = 6.3e-18
Identity = 89/322 (27.64%), Postives = 144/322 (44.72%), Query Frame = 0

Query: 27  EIVSEEEMA---LIEAALAAAVGTARSSISCSSTTAIHSPSCSSQNLFNTSTQKRIKLNE 86
           E VSEE      L ++ L     T  SS S S     +        L  +   KR     
Sbjct: 8   ETVSEEASGFSDLSDSELLDLEDTQESSASASKPGPSYELPGKDDKLIRSPKWKRRLDVS 67

Query: 87  SLLHRFRRKKGLAVTDITGTEWCEKQMEFSLLFGSRKT---TKAMKAGIARHAMLEAEVV 146
           S + RF  K  L VTD++   WCE+QM +   F    T   +  +  G + H   E EV 
Sbjct: 68  SPMERFHLKY-LYVTDLSTQNWCEQQMVYGKEFSGFLTPEKSAILDTGASIHLARELEVH 127

Query: 147 EKVKVQVKSAEDRWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGV 206
           + V + + S ED WA+K LN +  +  L  EG  RE    F V        VEGV +VGV
Sbjct: 128 DLVSIPITSKEDAWAVKFLNILSMIPTLQSEGRIRE----FPV-----FAEVEGVLLVGV 187

Query: 207 IDEIRMLEIDSGKIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQ 266
           IDE+      SG++  L + KTR    +P++ Q++    Q+  YKY+ D+++    ++  
Sbjct: 188 IDELHYTA--SGELE-LTELKTRGNPVLPSDAQKKKDYFQVSLYKYIFDAMVQGKVTAAS 247

Query: 267 FFDFFSLNPYSPLSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLP-PAHNQLLLRYESQ 326
                 L+P  PL   ++      G++ K+L D++       ++   P  + L + Y  Q
Sbjct: 248 LIHHTKLDPEKPLGPSVLRHAQQGGYSVKSLGDLIELVFLSLTLSDLPLIDSLKIEYVHQ 307

Query: 327 KDQSVIVEDEFAYDHDWLKSQL 342
              +V+  +  A+    ++S++
Sbjct: 308 GTATVLGTEMVAFAEKEVRSKV 316

BLAST of Sgr023368 vs. ExPASy Swiss-Prot
Match: Q9CXP9 (Exonuclease V OS=Mus musculus OX=10090 GN=Exo5 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 7.0e-17
Identity = 76/262 (29.01%), Postives = 123/262 (46.95%), Query Frame = 0

Query: 84  SLLHRFRRKKGLAVTDITGTEWCEKQMEFSL-LFGSRKTTKA--MKAGIARHAMLEAEVV 143
           S + RF  K  L VTD+    WCE QM +   L GS    KA  +  G + H   E E+ 
Sbjct: 71  SPMERFHLKY-LYVTDLCTQNWCELQMVYGKELPGSLTPEKAAVLDTGASIHLAKELELH 130

Query: 144 EKVKVQVKSAEDRWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGV 203
           + V V + + ED WA+K LN +  +  L  EG  RE    F V      G VEG+++VGV
Sbjct: 131 DLVTVPIATKEDAWAVKFLNILAMIPALQSEGRVRE----FPV-----FGEVEGIFLVGV 190

Query: 204 IDEIRMLEIDSGKIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQ 263
           IDE+       G++  L + KTR    +P   Q++    Q+  YKY+ D+++    +   
Sbjct: 191 IDELHY--TSKGELE-LAELKTRRRPVLPLPAQKKKDYFQVSLYKYIFDAMVQGKVTPAS 250

Query: 264 FFDFFSLNPYSPLSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLP-PAHNQLLLRYESQ 323
                 L    PL   ++      G + K+L D++       ++   PA + L L Y  Q
Sbjct: 251 LIHHTKLCLDKPLGPSVLRHARQGGVSVKSLGDLMELVFLSLTLSDLPAIDTLKLEYIHQ 310

Query: 324 KDQSVIVEDEFAYDHDWLKSQL 342
           +  +++  +  A++   +KS++
Sbjct: 311 ETATILGTEIVAFEEKEVKSKV 319

BLAST of Sgr023368 vs. ExPASy Swiss-Prot
Match: Q9H790 (Exonuclease V OS=Homo sapiens OX=9606 GN=EXO5 PE=1 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.5e-14
Identity = 71/262 (27.10%), Postives = 118/262 (45.04%), Query Frame = 0

Query: 84  SLLHRFRRKKGLAVTDITGTEWCEKQMEFSLL---FGSRKTTKAMKAGIARHAMLEAEVV 143
           S + RF  K  L VTD+    WCE Q  +      F + +    +  G + H   E E+ 
Sbjct: 71  SPMERFHLKY-LYVTDLATQNWCELQTAYGKELPGFLAPEKAAVLDTGASIHLARELELH 130

Query: 144 EKVKVQVKSAEDRWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGV 203
           + V V V + ED WA+K LN +  +  L  EG  RE    F V      G  EGV +VGV
Sbjct: 131 DLVTVPVTTKEDAWAIKFLNILLLIPTLQSEGHIRE----FPV-----FGEGEGVLLVGV 190

Query: 204 IDEIRMLEIDSGKIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQ 263
           IDE+       G++  L + KTR    +P E Q++    Q+  YKY+ D+++    +   
Sbjct: 191 IDELHYTA--KGELE-LAELKTRRRPMLPLEAQKKKDCFQVSLYKYIFDAMVQGKVTPAS 250

Query: 264 FFDFFSLNPYSPLSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLP-PAHNQLLLRYESQ 323
                 L    PL   ++      GF+ K+L D++       ++   P  + L + Y  Q
Sbjct: 251 LIHHTKLCLEKPLGPSVLRHAQQGGFSVKSLGDLMELVFLSLTLSDLPVIDILKIEYIHQ 310

Query: 324 KDQSVIVEDEFAYDHDWLKSQL 342
           +  +V+  +  A+    +++++
Sbjct: 311 ETATVLGTEIVAFKEKEVRAKV 319

BLAST of Sgr023368 vs. ExPASy Swiss-Prot
Match: F1Q514 (Exonuclease V OS=Danio rerio OX=7955 GN=exo5 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 3.6e-13
Identity = 80/285 (28.07%), Postives = 132/285 (46.32%), Query Frame = 0

Query: 77  KRIKLNE---SLLHRFRRKKGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKA----GI 136
           KR  L+E   S + RF RK+ L+VT +    WCE +  ++LL    K  +  +     G 
Sbjct: 81  KRKSLHENSFSPMQRF-RKQHLSVTLLCDQTWCEMKSVYNLLKPHIKRKEMQRTEVQIGQ 140

Query: 137 ARHAMLEAEVVEKVKVQVKSAEDRWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRM 196
             H   E E+ + V V +++ ED  A+KLLN +  +  LL  G   + + +F V      
Sbjct: 141 EIHLSRELEIQDVVPVDIRTREDGEAVKLLNMLHMI-PLLEAG---QRVREFPV-----F 200

Query: 197 GLVEGVWMVGVIDEIRMLEIDSGKIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLD 256
           G+ EGV+++GVIDE  ++    G++ +L + KTR  +++P+  Q +    Q+  YK L D
Sbjct: 201 GVQEGVFIMGVIDE--LMYNQKGEL-VLNELKTRRQNSLPSSAQDKVNCFQVGLYKLLFD 260

Query: 257 SLIPDGFSSRQFFDFFSLNPYSPLSEEIMESTSSLGFTAKTLDDVLR--CYTNCCSMLPP 316
            L+         FD   L     L   +     +LG  A+T ++++     T  CS L P
Sbjct: 261 GLVRGEMKKNHIFDHLKLRSAQILGAGVQAHAKNLGVHARTFEELVETLLITVSCSDL-P 320

Query: 317 AHNQLLLRYESQKDQSVIVEDEFAYDHDWLKSQLDPCSSLAWTAR 353
             + L + Y  Q     I      +D   ++ +L    S  WT +
Sbjct: 321 CIDLLQIEYFHQGSNGPIGTRVAPFDEAQIRGELQAYLSY-WTGQ 350

BLAST of Sgr023368 vs. ExPASy TrEMBL
Match: A0A6J1J5D7 (exonuclease V, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111483632 PE=3 SV=1)

HSP 1 Score: 488.0 bits (1255), Expect = 3.2e-134
Identity = 267/370 (72.16%), Postives = 289/370 (78.11%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP NDD NT++ VPNIPVEIVS+EEMALIEAALAAAV TAR     SS++AI
Sbjct: 22  MADSSSGLPPYNDDDNTHKGVPNIPVEIVSDEEMALIEAALAAAVTTAR-----SSSSAI 81

Query: 61  HSPSCSSQNLFNT----------------------------STQKRIKLNESLLHRFRRK 120
           H PSCSSQNLFNT                            STQK+IK+NESLL RFRR 
Sbjct: 82  HPPSCSSQNLFNTRSIHSITLLSKRGLPSSQLDVEDLCKSSSTQKKIKVNESLLQRFRRN 141

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KGLAVTDIT TEWCEKQME  LL G RK TKAM+AGIARHAMLEAEVV+KVKVQVKSAED
Sbjct: 142 KGLAVTDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVKSAED 201

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF FGVNQLLFEGLTREL           MGLVEGVWMVGVIDEI+MLEI + 
Sbjct: 202 NWALKLLNFTFGVNQLLFEGLTREL---------PVMGLVEGVWMVGVIDEIQMLEIGTC 261

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           K+PML+DTKTR   TIP EPQQRNGKLQLMCYK+LLD LI DGFS+RQFFDFFSLNP+S 
Sbjct: 262 KVPMLIDTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSI 321

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI +S++S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+I EDEFAY
Sbjct: 322 LSEEIADSSASFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIREDEFAY 377

BLAST of Sgr023368 vs. ExPASy TrEMBL
Match: A0A6J1FT99 (LOW QUALITY PROTEIN: exonuclease V, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111448276 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 5.5e-134
Identity = 267/370 (72.16%), Postives = 288/370 (77.84%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP NDD NT++ VPNIPVEIVS+EEMALIEAALAAAV TAR     SS++AI
Sbjct: 22  MADSSSGLPPFNDDDNTHKDVPNIPVEIVSDEEMALIEAALAAAVTTAR-----SSSSAI 81

Query: 61  HSPSCSSQNLFNT----------------------------STQKRIKLNESLLHRFRRK 120
           H PSCSSQNLFNT                            STQK+IK+NESLL RFRR 
Sbjct: 82  HPPSCSSQNLFNTRSIHSITLLSKRGLPSSQLDIEDLCKSSSTQKKIKVNESLLQRFRRT 141

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KGLAVTDIT TEWCEKQME  LL G RK TKAM+AGIARHAMLE+EVV+KVKVQVKSAED
Sbjct: 142 KGLAVTDITRTEWCEKQMELCLLSGVRKKTKAMEAGIARHAMLESEVVKKVKVQVKSAED 201

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF FGVNQLLFEGLTREL           MGLVEGVWMVGVIDEI+MLEI + 
Sbjct: 202 NWALKLLNFTFGVNQLLFEGLTREL---------PVMGLVEGVWMVGVIDEIQMLEIGTC 261

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           K+PML+DTKTR   TIP EPQQRNGKLQLMCYK+LLD LI DGFS+RQFFDFFSLNP+S 
Sbjct: 262 KVPMLIDTKTRMRHTIPAEPQQRNGKLQLMCYKFLLDCLICDGFSTRQFFDFFSLNPHSI 321

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI +ST S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+I EDEFAY
Sbjct: 322 LSEEIADSTVSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIGEDEFAY 377

BLAST of Sgr023368 vs. ExPASy TrEMBL
Match: A0A6J1CEQ6 (exonuclease V, chloroplastic isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011013 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.0e-132
Identity = 265/370 (71.62%), Postives = 291/370 (78.65%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M   GS  PP NDD +T+E VPNIPVEIVS+EEMALIEAALAA    ARSSIS SS++AI
Sbjct: 23  MADSGSGLPPFNDDESTHEGVPNIPVEIVSDEEMALIEAALAA----ARSSISSSSSSAI 82

Query: 61  HSPSCSSQNLFN----------------------------TSTQKRIKLNESLLHRFRRK 120
           +S S SS+ LFN                             STQKR+K+NESLLHRFRRK
Sbjct: 83  NS-SSSSKILFNARSIQSITLLSKRGLSAAHPDIEDLCKTASTQKRVKVNESLLHRFRRK 142

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           KG AVTD TGTEWCEKQ  FSL  GSR+ TKA++AGIARHAMLEAEVV+K KVQVKSAED
Sbjct: 143 KGFAVTDFTGTEWCEKQKVFSLHSGSREKTKAVEAGIARHAMLEAEVVKKAKVQVKSAED 202

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
           RWALKLLNF FGVNQLLFEGLTREL           MGL+EGVWMVGVIDE+RMLE D+G
Sbjct: 203 RWALKLLNFTFGVNQLLFEGLTREL---------PVMGLIEGVWMVGVIDEVRMLETDTG 262

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           ++ MLVDTKTR+ DT+P EPQQRNGKLQLMCYKY+LDSLIPDGFSSRQFFDFFSLNPYS 
Sbjct: 263 RMLMLVDTKTRSRDTVPAEPQQRNGKLQLMCYKYILDSLIPDGFSSRQFFDFFSLNPYST 322

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI ES +S GFTAKTLDDV+R YTNCCSMLP  HN+LLLRYESQKDQS+IVE EF Y
Sbjct: 323 LSEEITESITSFGFTAKTLDDVIRYYTNCCSMLPSTHNKLLLRYESQKDQSIIVEVEFEY 378

BLAST of Sgr023368 vs. ExPASy TrEMBL
Match: A0A0A0KC56 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G290860 PE=3 SV=1)

HSP 1 Score: 453.0 bits (1164), Expect = 1.2e-123
Identity = 248/370 (67.03%), Postives = 282/370 (76.22%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP  DD NT+E  PNIPVEIVS+EEMALIEAALAAAV T+R     SS++ I
Sbjct: 1   MADSHSGLPPFKDDDNTHEGFPNIPVEIVSDEEMALIEAALAAAVTTSR-----SSSSVI 60

Query: 61  HSPSCSSQNLFNTS----------------------------TQKRIKLNESLLHRFRRK 120
           HS S SS +LFNT                             TQK+IK+NESLL RF+R 
Sbjct: 61  HSTSSSSHSLFNTRSIHSITLLSKRGIHSTQPDIEDLGKIPITQKKIKVNESLLQRFKRN 120

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           K LAVTDIT  EWCEKQMEFSLL G RK TKAM+AGIARHAMLEAEVV+KVKVQV+S ED
Sbjct: 121 KPLAVTDITRMEWCEKQMEFSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVQSIED 180

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF+ GV+QL+ EGLTREL           MGLVEGVW+VG+IDEI+MLEID+ 
Sbjct: 181 IWALKLLNFIVGVHQLVLEGLTREL---------PVMGLVEGVWIVGIIDEIQMLEIDTT 240

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPDGFSSRQFFDFFSLNPYSP 300
           KIPML+DTKTR  DTIP  PQ+RNGKLQLM YK+LLD+LI DG S RQFFDFFSLNP+S 
Sbjct: 241 KIPMLIDTKTRVRDTIPAVPQRRNGKLQLMFYKFLLDNLISDGLSIRQFFDFFSLNPHST 300

Query: 301 LSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFAY 343
           LSEEI ++T+S+GFTAKTLDDV+R Y NCCSMLPP HNQLL+RYESQKDQS+IVED+FAY
Sbjct: 301 LSEEISDTTTSIGFTAKTLDDVVRYYINCCSMLPPTHNQLLIRYESQKDQSIIVEDKFAY 356

BLAST of Sgr023368 vs. ExPASy TrEMBL
Match: A0A5A7SXN1 (Exonuclease V OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00520 PE=3 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 3.4e-123
Identity = 251/371 (67.65%), Postives = 281/371 (75.74%), Query Frame = 0

Query: 1   MTGQGSEFPPLNDDGNTNESVPNIPVEIVSEEEMALIEAALAAAVGTARSSISCSSTTAI 60
           M    S  PP  DD NT+E  PNIPVEIVS+EEMALIEAALAAAV T+R     SS++ I
Sbjct: 1   MADSHSGLPPFKDDDNTHEGFPNIPVEIVSDEEMALIEAALAAAVTTSR-----SSSSVI 60

Query: 61  HSPSCSSQNLFNTS----------------------------TQKRIKLNESLLHRFRRK 120
           HS SCSS NLFNT                             TQK+IK+NESLL RF+R 
Sbjct: 61  HSTSCSSHNLFNTRSIHSITLLSKRGIDSSQPDIEDLGKIPITQKKIKVNESLLQRFKRN 120

Query: 121 KGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLEAEVVEKVKVQVKSAED 180
           K LAVTDIT  EWCEKQMEFSLL G RK TKAM+AGIARHAMLEAEVV+KVKVQV+S ED
Sbjct: 121 KPLAVTDITRMEWCEKQMEFSLLSGVRKKTKAMEAGIARHAMLEAEVVKKVKVQVQSFED 180

Query: 181 RWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVWMVGVIDEIRMLEIDSG 240
            WALKLLNF+ GV+QL+ EGLTREL           MGLVEGVW+VG+IDEI+MLEI++ 
Sbjct: 181 IWALKLLNFIVGVHQLVLEGLTREL---------PVMGLVEGVWIVGIIDEIQMLEINTT 240

Query: 241 KIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLI-PDGFSSRQFFDFFSLNPYS 300
           KIPML+DTKTR  DTIP  PQ+RNGKLQLM YK LLD+LI  DGF  RQFFDFFSLNP+S
Sbjct: 241 KIPMLIDTKTRVRDTIPAVPQRRNGKLQLMFYKLLLDNLISSDGFPIRQFFDFFSLNPHS 300

Query: 301 PLSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLLRYESQKDQSVIVEDEFA 343
            LSEEI +S++S GFTAKTLDDV+R Y NCCSMLPP HNQLLLRYESQKDQS+IVED+FA
Sbjct: 301 TLSEEIADSSTSFGFTAKTLDDVVRYYINCCSMLPPTHNQLLLRYESQKDQSIIVEDKFA 357

BLAST of Sgr023368 vs. TAIR 10
Match: AT5G60370.1 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; CONTAINS InterPro DOMAIN/s: Defects-in-morphology protein 1-like, mitochondrial (InterPro:IPR019190); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57110.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 302.8 bits (774), Expect = 3.7e-82
Identity = 173/326 (53.07%), Postives = 223/326 (68.40%), Query Frame = 0

Query: 22  PNIPVEIVSEEEMALIEAALAAAVGTARS---SISCSSTTAIHSP-SCSSQNLFNTSTQK 81
           P IP+EIVSEEEMA+++AALAA+     S   S+S S  TA  SP +  S  LF+     
Sbjct: 40  PEIPIEIVSEEEMAILDAALAASRSILPSVIRSVSPSRITAGGSPKTIRSITLFSKRKLS 99

Query: 82  RIK-LNESLLHRFRRKKGLAVTDITGTEWCEKQMEFSLLFGSRKTTKAMKAGIARHAMLE 141
               + ES LHRFRR + L VTD+TGTEWCEKQME  L FG RK  KAMK G ARH  LE
Sbjct: 100 ACSDIEESYLHRFRRNQALGVTDLTGTEWCEKQMENVLCFGRRKVNKAMKVGQARHLQLE 159

Query: 142 AEVVEKVKVQVKSAEDRWALKLLNFVFGVNQLLFEGLTRELLSDFLVALINRMGLVEGVW 201
            EVV+KV+V+V+S ED+WALKLLN + GVNQ LFEG TRELL          +G V G W
Sbjct: 160 EEVVKKVRVKVESNEDKWALKLLNSIAGVNQFLFEGRTRELL---------LLGFVGGQW 219

Query: 202 MVGVIDEIR--MLEIDSGKIPMLVDTKTRALDTIPTEPQQRNGKLQLMCYKYLLDSLIPD 261
           +VG+IDE+R    E  S   P+L+DTKTR  DT+P EPQ+RNG+LQLM YK L D+++ +
Sbjct: 220 IVGIIDELRKAYAEESSDSGPILIDTKTRLRDTLPAEPQRRNGRLQLMLYKLLWDTIVKE 279

Query: 262 GFSSRQFFDFFSLNPYSPLSEEIMESTSSLGFTAKTLDDVLRCYTNCCSMLPPAHNQLLL 321
           GF +  FFD+FSLN    LS+++ +  ++ G  A+TL++++R Y N   MLP A+NQLLL
Sbjct: 280 GFPTGPFFDYFSLNRNYVLSQDVRDHIANAGIKAQTLEEIVRYYENTFQMLPIANNQLLL 339

Query: 322 RYESQKDQSVIVEDEFAYDHDWLKSQ 341
           +YE QKDQS+I E  F +DH+W+ S+
Sbjct: 340 KYEFQKDQSIIAEIRFNHDHEWVMSK 356

BLAST of Sgr023368 vs. TAIR 10
Match: AT3G57110.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G60370.1); Has 16 Blast hits to 16 proteins in 5 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 16; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 49.7 bits (117), Expect = 5.7e-06
Identity = 38/87 (43.68%), Postives = 53/87 (60.92%), Query Frame = 0

Query: 22  PNIPVEIVSEEEMALIEAALAAAVGTARS---SISCSSTTAIHSP-SCSSQNLFN-TSTQ 81
           P IP+EI++EEE+ +++AALAA+     S   S+S S   A  SP +  S  LF+     
Sbjct: 40  PEIPIEIINEEEIVILDAALAASHSILPSVIRSVSPSQMIAGGSPITIRSITLFSKRKLS 99

Query: 82  KRIKLNESLLHRFRRKKGLAVTDITGT 104
             + + ES LHRFRR + L VTD+TGT
Sbjct: 100 ACLYIEESYLHRFRRNQSLGVTDLTGT 126