Sgr023327 (gene) Monk fruit (Qingpiguo) v1

Overview
NameSgr023327
Typegene
OrganismSiraitia grosvenorii (Monk fruit (Qingpiguo) v1)
DescriptionDNA ligase
Locationtig00000892: 2275306 .. 2284080 (+)
RNA-Seq ExpressionSgr023327
SyntenySgr023327
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGTGGCGCGAAGCAGGGAGAATAGGATCGGAATAAGCGAGCATCATGTTCGGATTCCGACAGAGTTTGAAGAGTTCGAGTTTCACAATTTCATCTCCCTGTAAAATGGATATTCGTATTATTTAAAGAATAAACTCACGGCGACGGCTGAAGCGCGGTGGCCTCTCTCTTACTGTGCGGGTAAGGGCGGAAGCCGACGGTAATTAACAATTACAGCGTGATCAAAACCCAAAACCATTCCCGCCATTTCTCACTCTTCCGGCTGCCCTGTCTTCTCTCCATCTTCGCTCTCTCTGAAATCGCTTCTTTCCGTTTGCAATATATGCGATTGATGCTTTCACTGCGTTCATGTTACTATCTTTGCCCCGTCACATTGCCTCATCCCCAATTCAAGAAACTCATCTTCTCCAACAAACCTCCCTCTTTCAAACCCATCTTCCAGAATTTTGTTTCCGCAATGACTTCTCGTCCTTCCGCTTTCGATGCTCTCATGTCTGGTGCTCGGGCCGCTGCCAAGAAGAAGTCTGCCGCTTCTTCTCCCAAGAAGCGTAAAGCCCTAGATTCTTTAGACCCTCCTCCAAAACCTAGCTCTTCCCAGACTCTGGAGTCGACGCCGCCTGTTGAATCGCAGTGCGGCAACTCAACTTTGGACAATCATGGAGGTTCTCATCTGGGAACGCCCGAGAACGCTGTAGATTTGGGAGAAACCAAGTTGCGTCAAGATAATGGTGGCAGTGTCGGAACTAAGAATGTGCTGGCGGCGAACAAAACTTGCGCGACAAGTGTTCAAGAAAGAACTGCGAAATTGAAGAGCTGTGTTCCAGAGTTGAAAATGAAGGCCAAGGATTTTAACTCAAAAAATGTTGCATGCTGGAAAGAGGGCGAACGGGTCCCCTTTTTGTTTCTGTGTTTGGGCTTCGACATGGTTTCTGAGGAGTCTAGTAGGATTACTATCACCGATATAGTGTGTAATATGTTGAGGACCGTCATGGATACTACACCCGATGACTTGGTGGCCACTGTGTATCTTTTGGCGAATAAGATTGCTCCAGCCCACGAAGGGCTTGAGTTGGGGATTGGGGATGCTTCTATTATCAAAGCTCTTTCTGAGGCATTTGGAAGGACAGAGGCACAGGTTAAAAAACAGTACAAGGTGAGGTGGCTATTGACCTTCATCCTCTAAATTAGGATAAAATTCCCAAGGTCTACACTGATATTTTTTTGGAGACGATTGCATTATGGCAATTCAGAGATTATTAATAGGAATAAGTTACGACAACAATTCGGTTGTCCCTAATCTTGGATTAATGTTCGTTGCAATGTGTTTTACAGAGTTTATAATTGAGAAAGAATGGATGATAGAGGCGCCTTTTCTCTTTATGTATTAAAATGTTTTCACGTTGTTGACCGTACAAGTGTCTCAAGAATATCGCAATTAATAAGCCTAATTGATTCCTGGTCTATATGGAATTTGTCACGGAATCCTATTTGAACAATTAACATTTGATGTTTCAATGGCTCAATTGTTTGCTTCCCTGGTTTATTTGACAGGAATTGGGAGATTTAGGTCTTGTTGCTAAAGCAAGCCGTTCATCGCAGTCTATGATGCGCAAGCCTGATGCTTTAACTATTGCCAAAGTGTTTGACACATTTCGGCTGATTGCTAAGGTGTGTTAGGCTTGTGATTTTAATTGCTTAATGCATAACCAGTAAACTAGGGTTCATTGGTTAGTTTGACTGTGAACTATATCGAGTGGAGTTACTATCTAGTAAATTCAGCTTTTCCCTAAGTTATACAAGTATAATTTTTTTCTAATTTTATGGCAATCTTTTTCTATAAATGTGGGGCATGGGATCTGGCCTGTATGGCGTGCTGTAAAATTTGTGTTGATAGAGTCGTATATATTTGAAAATTGCCTTTCGACCTTTTTGAATGTTCTCAGGAATCTGGCAAGGATAGTCAAGAGAAGAAAAAGAACCATATCAAGTCACTGCTTGTTGCTGCCACTGACTGTGAACCTTTATATCTGATTAGATTGCTGCAGGTTATGTCTCAGCTTTGTTAGAATTAAGATTTTCAGTTCCTTTTGTCTAATGTCAAGTTTCTTTCTTATCATGCAGACAAAATTACGAATTGGACTTGCTGAGCAGACTCTTTTAGCTGCATTAGGGCAAGCTGCTGTATATGCTGAAAAACACTCTACACCACCACCAAACATTCAATCTCCTCTAGAAGAGGTGATTTCATGTACTCCGTGTCTGTGGGAACATTTGCTATAATTCTGCCAGTCCTTTCTTTTTGATCATTATCTCTCTGTGCTATGTTATTATTATAATTCTGAGACTCTAAGCTATTTTGATGAAGTATCATGTTTTGAGCTACTCCTACACCTTGGTTTGGTTACTGGGTTACTTGCATAGTGTCACTTACTGAGCTTTAAGATGTTCTTTTCATTGGAAGGTTCTAACGTTATGTTTGCATCAGGCTGCCAAGATTGTAAAGCAGGTGTATTCTATACTCCCAGTTTATGATAAACTTGTCCCTGCACTTCTTAGTGGTGGTGTGTGGAATCTTTCAAAAACGTGTAACTTTACACCGGGTATTCCAATTGGACCCATGCTGGCAAAACCAACAAAGGGAGTGTCAGAGATCTTAAATAAATTCCAGGATTTGGAATTTACTTGTGAATACAAATATGATGGGGAACGTGCCCAGGTGACTGAATGAAAAACTTAAAAAATGTGTTCTTCTGCTGCTATTTACATATTCATATGGTATGATTTCACTCCCTTTATGCAGATACATTACATGGAAAATGGTTCAGTTGAGATATACAGTCGAAATGCTGAAAGGAACACTGGAAAGTTCCCTGATGTTGTTATCACAGTATCAAGGTAAAGTGACCTGGCAGTGTTTACTTCATTTAGAATTATATGAGTCAATTGGCTTTTTAAATGATTGATTCCAGTTTAGAAGCAATGCATAAGACAGTTCAGATTTCTTATATTTCTTCAATTAGGAAGCAATAAATTACTTGACTATTGCCGTTTCAGTTTGTTCTTTTCAGTATGATTTTTCCATTTCATCTTTTATTTTGATCCTTTATTGCATTGATTTTCATTGATTTTTTAATTGAGACCAGTTACAGGAAGTTGGATATGATGCAATACATTGTTCTTGTTGATGTTCCTAATACATAATACCTATTTCTCTACATTCATAATTTTTCATTAAATATTCTGTTAATTTTTAAATTTACTGCATTTTTGCAGGGTAAAGAAACCATCTGTTAAATCATTTATTTTGGATTGCGAACTTGTTGCTTATGATCGTGGAAAACAGAAGATTCTGCCTTTCCAGGTTTACAGTATATGTTACCTGGTTTATAGCATATGACAGATATGTTTGTTTTATGATATATTTTTTAATCTTAAATATGGAGTGGTCCTTTATTAATATCTACTTTTGTTTTTTGAATATCTTTGTGTAGCTGTCATGTCATAAAAATCAGATCAAAGAAAATCACATTAAACAGCCAGAACTGGGAAGATTCAAATGACCATGCAGTAATCAGTCTGAAATCATTTTCTGTGCTACTAAAATATTCCTAAGCACATTCATCATTCTCCTTTTCTAGAAACCTGCACTTTCTCGTTTTTGGTTTTCACCTTAAAACCATTTTTAGTAGACATTCTCTTCTTATCCCATTGGTCTTTTGCCATTATCTCTAGCTTGGCTTCTAGAGTCACTACAGAAGTAACACTAACCCTGAAATTTGTGACAGATTCTGAGTACTCGAGCTCGCAAGAACGTGGCCGTGAGTGATATTAAGGTTGATGTGTGTGTATTTGCTTTTGATTTATTGTATCTTGATGGTCAACCACTTATCCAGAGAGAACTTAATGTTCGTCGAGAGGTAGAATTTCTCCCTCTCTCCCCTTCTTGTGCATGTGTGCGTGGTACACAACTGGCATCGATGATTACTAATGTTTTAGCCTTGCTGTATCTGTAGTCATTTTATGTTTTTTTATATTAAAGATATTAAGGATCAATTTTAATGACTTATTATCTGTGCCATCTCTCGTATCATTGAATTTCCACCAATTCGTTTTGCCACAATAGTTTTAGCATTCAAAATTATCTGTCTGAAATGATACGTTTGGAGCATGAATTGATTAACAAACTAAACTTATGGACTACTCTTGTAAAGATGTTCGGATTTTTCTAAGATTGCAATATAGAGCTCTAAGTGTATTGTTACATGGCCATGAGTTGTTCCTTTTTCTTTTAACTGATATGCAGTTCTTCAACATGCTTTGTTTTGGTCTCTGCAAATGTTTGTATCATTTATAACTGGGGTTCTGTACTTTGCAGCTTCTCTATGACTTATTTATGGAAGAACCTGGATATTTTCAATTTGCCACAGCAATAATAACAAATGATCTTGAGGAAATACAAAAGTTCCTTGACGCATCTGTTGATCAAAGGTGTGTTGCCTTTTCCTGGTGTACAAGGTTTACATTTTATGTCAATGTTTTTCTACTATGTAGTGGACCATGATCTCAAAAACCAAAACATTTGTGTTAAATGGGCGTAAGCTATTTTGAGCTGAAGTTACTATCTGTTATATATTTATGCATGGGAGATTGTGTATTTGCTTCATAACCTTACCTTATCATGTTTTCTCTTGCTTTCTTGTTTGACTATTTTATTCTTCTCGCATTCTATTAATTAATCTTAGTCTGCAGTTGTGAGGGCTTAATTATCAAAACACTGAATAGAGATGCTACTTATGAGCCTTCCAAGAGGTCTCATAACTGGTTAAAACTGAAGAAGGATTACATAGACAAGTAAGTGCTTCATGTATGTCTGTATAAATGTTGTATGATAATCTCTACCGAGCTTATGGATGCCTGTACACTGCAGCATAGGGGATTCACTTGATCTGGTACCAATTGCTGCTTTCCATGGCCGTGGGAAGCGTACAGGTACTATAACCGGAAAGCAGTTTTAGCATATGCATGCAATAATTGCACGTGGTATATAATTATCTAAAATAATTGGTTTAAATCCAGTGGCTCTCTTCCTGTAATATCCAACCTATATGTGCGCAAGCATGTATGCGTGTATATGAACTTGTGTTCATGTTGTTCTTATGCCACTAATATAATATATCCCTAGGTGTCTATGGTGCATTCCTCCTTGCTTGTTATGACAATGACAATGAAGAATTTCAAAGCATTTGTAAGATAGGTAAGCGATAAGGAATTCTTTAGCTTTTGTTTTTCCACTGATTAACCGTCCGTGCTTGTTCTTTTTTTTTTCTTTTTTTTTTTTGTATTTTAACTGTCTAATATTAAGTTTTGATGACTGCTAGGGACCGGTTTTTCTGAAGCAGTGCTTGAGGAACGTTCTGCTAGTCTCCGTTCTAAAGTCATTCCTGAACCAAAGGTCTGTCTTCTAATTCCTATTATTGATGTGAACATTGGGTTCCCCATATGATTCTACTTTTTAATTCGATATTTTCTTGTCAATTTTAGTCATATTATCGGTATGGAGATACAATCAATCCAGATGTTTGGTTTGAGCCTACTGAGGTAACCATGATGACTTCTTGTACTGATATCTTATTTCATGCAGTCTTTTCTTACGCCTTTTGCCTCTTTTCCTCTTAAGGCCATGCTGTATCTTTTTAGCCCCCTAAAAGCAATTATCAGCAATTTGAAAATTTATTCCCCTTCAATGGCGTGGTACTTGAAGGTGGCGTGGGTTTTTCTGCCCCTTCCTTTGCGACAAGAAAAGTTTGAAAATAACATGAAAAAGGGGCTTTTGAAAGAGATTTCAAAATCAATAGATTCAGCCGAGAATTACATTACTAGAGTTGAAACTTAATCTTTATTGGCAAAGTGACTGGAGTTGTTCTCTTATTCATTCTTTTTTAATAATTCTGTTCCAGGTTTGGGAGGTTAAGGCAGCAGACTTGACTATTAGCCCAGTTCATCGTGCAGGAGTGGGCATTGTGGATTCTGATAAGGTTGTATTTGTGCACAAGTTTCTCTTTGTAATTTTCTCCCTCATCATTCTTCATTTCTTTCTATAGTCCTGATCTTTTTTACCATTCATGGCTTTCTGTGTAAAGGGAATTTCTCTTCGATTTCCACGTCTTGTTCGTGTAAGGAAAGACAAAAATCCAGAGGAAGCCTCATCATCAGAGATGGTGAATATTACATACCCTCTTACTAGCATTTTCATGATTAACAATGGATTAAATGACCGTTTAGAAAAATGGGAGAAACTTCCAAAAAGTTAACAAAGTTGCAAAAAAACCTCTCCAATGGGCACAAATGGTAGAAAGAGAATTGTTGCCATAAAAAAAAAAAGGAGGAAATGAAAGCTAAAGTATCCCTAATCTTTTCGATATCTCTATGATTCTATCAATCTTTGGGAGTATATATTATTGTTCCACGGATTTCTCAATACTGTTCTTTTAGAACTTTTAACTATGCACACATTTCCTTTTGAATTAGGTTGCCGACATGTATAATGCTCAGAAACACAACCACCAAAACAATCAAGATGACAATGAAGACGACTGAGGAGTGAAGTTTTAACTCGTTCCAAACATGTTGCATTCTCGATTTTCAACTGGAGAATTATTGGCAAATTACCATGTTTGGTGTCATGGCACATGCCATGTCTGCTTGGGCCTTGGGGTATGTTTCAGGTTCAACCGATCAGATTTTAGCTGATCCCGATCCTTCCTTGCAATACATAAAATGTTTTCCTGCCGTTGAGCTAATAGTTTCATGGCCCACATATGTTCTCATATCATCTTTAACAATGTAGACAAAAGGTGAAATCAATTTTTATGTTTCTTCTCTCATGACACTTGTACATTATGTTTCATGATATTATATGAGCTGTTTCTTGTATCGCATTCAATTGTTTCGAATCGTATATTATGCCCCTTTCTAGGGGACACTGGTATTGCACGTACGTAGACAAGTATTTTGCATCCATTTGATATGCTTGTAATAATCATCGCAAATTTATCTTTGAATTTTAGTAGGCCTGACCATGATGAGCCTGGACTAGGGCTTATATATGTTTGTTCTCGACGTCCGGAAGCACTGTCCAAATGAAGAGACAAGATAAGCCCGACAGCCTAAGCCATGTCCGAGTGTGGTAATTTGATCAAAGTAGTCGTGATCAGAGGCCAAGTATAGTCTTCCCTGTTAACTTTAGCGAACATCACCGTGTTGACTGGGGCAGAGACAGCCCGCGATCACATGGGTCTGTGCTGAAATGGCTCGTTGGCTTCTTGTGTAGCCATTTGTGGTCAACTGGCAGAGATTTCGGAGCAATTTTTTCTCTTCCTTTTGTCCTCACTTAGAAATGCTTATGCAATTTGTAAGGGTTGATTTGGTTCTGAATGTTATCCGAAGACTGCGAAGAATGAAACTCTGCCACATGTTATATCTCTGTACTTAAAACATGAAAATTCTCCCCAGCCTTTAGGATCAAATTTTGCCCGTCAAAGCAATTCATTTAACCCCTCCTCCCAAGGAATTTCCAAAGATTTGGGAAACTCAGAGATCAGATTTGAACATAATTTTTGATGATACGTTTTTCCCCCCTTTATATGTATACACACATTAGGTGCGGGTGATTTTGAAATTGAACTTGAGATACACCTACAAACTTGTTGATAATGGTAATTATAATTGAACTTGCAATACTTTGTTCAACAAATTATTTATTTTGGACTGGCGACTGATCTGCAAGCAATTGCGTTGAGTGTTTGATGAGTTAGTATATTATGTTTTTTCTGTAACAATAGACAAGCAATTAAACACGAGAAATCACAGAATTGGAATTGCATCTTATATGGCTAAAACAATTCTGGAAAAATTCGACCCCGTTTCTCAGACGGAGTATCAAAAAGAGAAAAAAAGAGTGTGAAGTAAAAGGGTGGCCATTAAAGCTTCACTCAAGGCAACTGAGTCAGAGTGAAATGTGATGACAGAAGGGGGAGAGTAGGGGAGACTGCTTCTGTTGTAGCTGCGTCAAACATAGGAGGAGAGAGTTTCTTACCTAAAACAAGCCTCTGTACAAGCACTAAAATCACCCACTAACCCAATTCTCTTTTCATCCTCATTAGCCCTTTAGTAATATTAGACTATAATTAAATTCTTCCTGCTCTCTCTTTTTGTAGTGGTCAGCAGGGAGCAAGCCTTTGGCACTTTGTCTCTGTTGGAGAATCAGAGCCTTCCGAGAAGACCCTTTTTCTGCTTCCGCAGTTGGTAATGTTGCTGCTGCAATTGAAGGTGATCGGAGAATCTCGGAGGAGCTGTGGCGGTGGCGGTGGCGGTGGCGTTGAAGGAATGGTCACCGGGGGGATTTCGAAGGATTCAAGTGGCGGTGGTGGGTGTCTCCACTCAGGCAGTGGTCCGGCCACAAGAAGAGTCTGAAGCAGAGGGCCTGCTTTCATCACTGCTTCCAAGAGCTTGCCTTTTTCAGGCAATGGCTTCTCTGGAACCAGTTCCACCGCCGCGTGCGGCACTTGTCCCGGTGGTGCTTGCGACAGAGCTGAAGGAAACTGTGGCGGCGGAATTGGGTCAATGGCAGGAGATGAGACAATGCTTTCTTCGCAATCTGATGAGGAGAAGCCATTGTTGGAGTCAATTCCTTTCCTTGGTTCATCTTCAATGCTGGAAATTCCTGAATGAGGATCTGTTCGCTGCTGCTGTTGCTGGAGTAAAAGCTTCTCAATGA

mRNA sequence

GGTGGCGCGAAGCAGGGAGAATAGGATCGGAATAAGCGAGCATCATGTTCGGATTCCGACAGAGTTTGAAGAGTTCGAGTTTCACAATTTCATCTCCCTCGTGATCAAAACCCAAAACCATTCCCGCCATTTCTCACTCTTCCGGCTGCCCTGTCTTCTCTCCATCTTCGCTCTCTCTGAAATCGCTTCTTTCCGTTTGCAATATATGCGATTGATGCTTTCACTGCGTTCATGTTACTATCTTTGCCCCGTCACATTGCCTCATCCCCAATTCAAGAAACTCATCTTCTCCAACAAACCTCCCTCTTTCAAACCCATCTTCCAGAATTTTGTTTCCGCAATGACTTCTCGTCCTTCCGCTTTCGATGCTCTCATGTCTGGTGCTCGGGCCGCTGCCAAGAAGAAGTCTGCCGCTTCTTCTCCCAAGAAGCGTAAAGCCCTAGATTCTTTAGACCCTCCTCCAAAACCTAGCTCTTCCCAGACTCTGGAGTCGACGCCGCCTGTTGAATCGCAGTGCGGCAACTCAACTTTGGACAATCATGGAGGTTCTCATCTGGGAACGCCCGAGAACGCTGTAGATTTGGGAGAAACCAAGTTGCGTCAAGATAATGGTGGCAGTGTCGGAACTAAGAATGTGCTGGCGGCGAACAAAACTTGCGCGACAAGTGTTCAAGAAAGAACTGCGAAATTGAAGAGCTGTGTTCCAGAGTTGAAAATGAAGGCCAAGGATTTTAACTCAAAAAATGTTGCATGCTGGAAAGAGGGCGAACGGGTCCCCTTTTTGTTTCTGTGTTTGGGCTTCGACATGGTTTCTGAGGAGTCTAGTAGGATTACTATCACCGATATAGTGTGTAATATGTTGAGGACCGTCATGGATACTACACCCGATGACTTGGTGGCCACTGTGTATCTTTTGGCGAATAAGATTGCTCCAGCCCACGAAGGGCTTGAGTTGGGGATTGGGGATGCTTCTATTATCAAAGCTCTTTCTGAGGCATTTGGAAGGACAGAGGCACAGGAATTGGGAGATTTAGGTCTTGTTGCTAAAGCAAGCCGTTCATCGCAGTCTATGATGCGCAAGCCTGATGCTTTAACTATTGCCAAAGTGTTTGACACATTTCGGCTGATTGCTAAGGAATCTGGCAAGGATAGTCAAGAGAAGAAAAAGAACCATATCAAGTCACTGCTTGTTGCTGCCACTGACTGTGAACCTTTATATCTGATTAGATTGCTGCAGACAAAATTACGAATTGGACTTGCTGAGCAGACTCTTTTAGCTGCATTAGGGCAAGCTGCTGTATATGCTGAAAAACACTCTACACCACCACCAAACATTCAATCTCCTCTAGAAGAGGCTGCCAAGATTGTAAAGCAGGTGTATTCTATACTCCCAGTTTATGATAAACTTGTCCCTGCACTTCTTAGTGGTGGTGTGTGGAATCTTTCAAAAACGTGTAACTTTACACCGGGTATTCCAATTGGACCCATGCTGGCAAAACCAACAAAGGGAGTGTCAGAGATCTTAAATAAATTCCAGGATTTGGAATTTACTTGTGAATACAAATATGATGGGGAACGTGCCCAGATACATTACATGGAAAATGGTTCAGTTGAGATATACAGTCGAAATGCTGAAAGGAACACTGGAAAGTTCCCTGATGTTGTTATCACAGTATCAAGGGTAAAGAAACCATCTGTTAAATCATTTATTTTGGATTGCGAACTTGTTGCTTATGATCGTGGAAAACAGAAGATTCTGCCTTTCCAGATTCTGAGTACTCGAGCTCGCAAGAACGTGGCCGTGAGTGATATTAAGGTTGATGTGTGTGTATTTGCTTTTGATTTATTGTATCTTGATGGTCAACCACTTATCCAGAGAGAACTTAATGTTCGTCGAGAGCTTCTCTATGACTTATTTATGGAAGAACCTGGATATTTTCAATTTGCCACAGCAATAATAACAAATGATCTTGAGGAAATACAAAAGTTCCTTGACGCATCTGTTGATCAAAGTTGTGAGGGCTTAATTATCAAAACACTGAATAGAGATGCTACTTATGAGCCTTCCAAGAGGTCTCATAACTGGTTAAAACTGAAGAAGGATTACATAGACAACATAGGGGATTCACTTGATCTGGTACCAATTGCTGCTTTCCATGGCCGTGGGAAGCGTACAGGTGTCTATGGTGCATTCCTCCTTGCTTGTTATGACAATGACAATGAAGAATTTCAAAGCATTTGTAAGATAGGGACCGGTTTTTCTGAAGCAGTGCTTGAGGAACGTTCTGCTAGTCTCCGTTCTAAAGTCATTCCTGAACCAAAGTCATATTATCGGTATGGAGATACAATCAATCCAGATGTTTGGTTTGAGCCTACTGAGGTTTGGGAGGTTAAGGCAGCAGACTTGACTATTAGCCCAGTTCATCGTGCAGGAGTGGGCATTGTGGATTCTGATAAGGGAATTTCTCTTCGATTTCCACGTCTTGTTCGTGTAAGGAAAGACAAAAATCCAGAGGAAGCCTCATCATCAGAGATGTGGTCAGCAGGGAGCAAGCCTTTGGCACTTTGTCTCTGTTGGAGAATCAGAGCCTTCCGAGAAGACCCTTTTTCTGCTTCCGCAGTTGGTAATGTTGCTGCTGCAATTGAAGGTGATCGGAGAATCTCGGAGGAGCTGTGGCGGTGGCGGATTCAAGTGGCGGTGGTGGGTGTCTCCACTCAGGCAGTGGTCCGGCCACAAGAAGAGTCTGAAGCAGAGGGCCTGCTTTCATCACTGCTTCCAAGAGCTTGCCTTTTTCAGGCAATGGCTTCTCTGGAACCAGTTCCACCGCCGCGTGCGGCACTTGTCCCGGTGGTGCTTGCGACAGAGCTGAAGGAAACTGTGGCGGCGGAATTGGGTCAATGGCAGGAGATGAGACAATGCTTTCTTCGCAATCTGATGAGGAGAAGCCATTGTTGGAGTCAATTCCTTTCCTTGGTTCATCTTCAATGCTGGAAATTCCTGAATGAGGATCTGTTCGCTGCTGCTGTTGCTGGAGTAAAAGCTTCTCAATGA

Coding sequence (CDS)

GGTGGCGCGAAGCAGGGAGAATAGGATCGGAATAAGCGAGCATCATGTTCGGATTCCGACAGAGTTTGAAGAGTTCGAGTTTCACAATTTCATCTCCCTCGTGATCAAAACCCAAAACCATTCCCGCCATTTCTCACTCTTCCGGCTGCCCTGTCTTCTCTCCATCTTCGCTCTCTCTGAAATCGCTTCTTTCCGTTTGCAATATATGCGATTGATGCTTTCACTGCGTTCATGTTACTATCTTTGCCCCGTCACATTGCCTCATCCCCAATTCAAGAAACTCATCTTCTCCAACAAACCTCCCTCTTTCAAACCCATCTTCCAGAATTTTGTTTCCGCAATGACTTCTCGTCCTTCCGCTTTCGATGCTCTCATGTCTGGTGCTCGGGCCGCTGCCAAGAAGAAGTCTGCCGCTTCTTCTCCCAAGAAGCGTAAAGCCCTAGATTCTTTAGACCCTCCTCCAAAACCTAGCTCTTCCCAGACTCTGGAGTCGACGCCGCCTGTTGAATCGCAGTGCGGCAACTCAACTTTGGACAATCATGGAGGTTCTCATCTGGGAACGCCCGAGAACGCTGTAGATTTGGGAGAAACCAAGTTGCGTCAAGATAATGGTGGCAGTGTCGGAACTAAGAATGTGCTGGCGGCGAACAAAACTTGCGCGACAAGTGTTCAAGAAAGAACTGCGAAATTGAAGAGCTGTGTTCCAGAGTTGAAAATGAAGGCCAAGGATTTTAACTCAAAAAATGTTGCATGCTGGAAAGAGGGCGAACGGGTCCCCTTTTTGTTTCTGTGTTTGGGCTTCGACATGGTTTCTGAGGAGTCTAGTAGGATTACTATCACCGATATAGTGTGTAATATGTTGAGGACCGTCATGGATACTACACCCGATGACTTGGTGGCCACTGTGTATCTTTTGGCGAATAAGATTGCTCCAGCCCACGAAGGGCTTGAGTTGGGGATTGGGGATGCTTCTATTATCAAAGCTCTTTCTGAGGCATTTGGAAGGACAGAGGCACAGGAATTGGGAGATTTAGGTCTTGTTGCTAAAGCAAGCCGTTCATCGCAGTCTATGATGCGCAAGCCTGATGCTTTAACTATTGCCAAAGTGTTTGACACATTTCGGCTGATTGCTAAGGAATCTGGCAAGGATAGTCAAGAGAAGAAAAAGAACCATATCAAGTCACTGCTTGTTGCTGCCACTGACTGTGAACCTTTATATCTGATTAGATTGCTGCAGACAAAATTACGAATTGGACTTGCTGAGCAGACTCTTTTAGCTGCATTAGGGCAAGCTGCTGTATATGCTGAAAAACACTCTACACCACCACCAAACATTCAATCTCCTCTAGAAGAGGCTGCCAAGATTGTAAAGCAGGTGTATTCTATACTCCCAGTTTATGATAAACTTGTCCCTGCACTTCTTAGTGGTGGTGTGTGGAATCTTTCAAAAACGTGTAACTTTACACCGGGTATTCCAATTGGACCCATGCTGGCAAAACCAACAAAGGGAGTGTCAGAGATCTTAAATAAATTCCAGGATTTGGAATTTACTTGTGAATACAAATATGATGGGGAACGTGCCCAGATACATTACATGGAAAATGGTTCAGTTGAGATATACAGTCGAAATGCTGAAAGGAACACTGGAAAGTTCCCTGATGTTGTTATCACAGTATCAAGGGTAAAGAAACCATCTGTTAAATCATTTATTTTGGATTGCGAACTTGTTGCTTATGATCGTGGAAAACAGAAGATTCTGCCTTTCCAGATTCTGAGTACTCGAGCTCGCAAGAACGTGGCCGTGAGTGATATTAAGGTTGATGTGTGTGTATTTGCTTTTGATTTATTGTATCTTGATGGTCAACCACTTATCCAGAGAGAACTTAATGTTCGTCGAGAGCTTCTCTATGACTTATTTATGGAAGAACCTGGATATTTTCAATTTGCCACAGCAATAATAACAAATGATCTTGAGGAAATACAAAAGTTCCTTGACGCATCTGTTGATCAAAGTTGTGAGGGCTTAATTATCAAAACACTGAATAGAGATGCTACTTATGAGCCTTCCAAGAGGTCTCATAACTGGTTAAAACTGAAGAAGGATTACATAGACAACATAGGGGATTCACTTGATCTGGTACCAATTGCTGCTTTCCATGGCCGTGGGAAGCGTACAGGTGTCTATGGTGCATTCCTCCTTGCTTGTTATGACAATGACAATGAAGAATTTCAAAGCATTTGTAAGATAGGGACCGGTTTTTCTGAAGCAGTGCTTGAGGAACGTTCTGCTAGTCTCCGTTCTAAAGTCATTCCTGAACCAAAGTCATATTATCGGTATGGAGATACAATCAATCCAGATGTTTGGTTTGAGCCTACTGAGGTTTGGGAGGTTAAGGCAGCAGACTTGACTATTAGCCCAGTTCATCGTGCAGGAGTGGGCATTGTGGATTCTGATAAGGGAATTTCTCTTCGATTTCCACGTCTTGTTCGTGTAAGGAAAGACAAAAATCCAGAGGAAGCCTCATCATCAGAGATGTGGTCAGCAGGGAGCAAGCCTTTGGCACTTTGTCTCTGTTGGAGAATCAGAGCCTTCCGAGAAGACCCTTTTTCTGCTTCCGCAGTTGGTAATGTTGCTGCTGCAATTGAAGGTGATCGGAGAATCTCGGAGGAGCTGTGGCGGTGGCGGATTCAAGTGGCGGTGGTGGGTGTCTCCACTCAGGCAGTGGTCCGGCCACAAGAAGAGTCTGAAGCAGAGGGCCTGCTTTCATCACTGCTTCCAAGAGCTTGCCTTTTTCAGGCAATGGCTTCTCTGGAACCAGTTCCACCGCCGCGTGCGGCACTTGTCCCGGTGGTGCTTGCGACAGAGCTGAAGGAAACTGTGGCGGCGGAATTGGGTCAATGGCAGGAGATGAGACAATGCTTTCTTCGCAATCTGATGAGGAGAAGCCATTGTTGGAGTCAATTCCTTTCCTTGGTTCATCTTCAATGCTGGAAATTCCTGAATGAGGATCTGTTCGCTGCTGCTGTTGCTGGAGTAAAAGCTTCTCAATGA

Protein sequence

VARSRENRIGISEHHVRIPTEFEEFEFHNFISLVIKTQNHSRHFSLFRLPCLLSIFALSEIASFRLQYMRLMLSLRSCYYLCPVTLPHPQFKKLIFSNKPPSFKPIFQNFVSAMTSRPSAFDALMSGARAAAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNSTLDNHGGSHLGTPENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKNVACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGIGDASIIKALSEAFGRTEAQELGDLGLVAKASRSSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLSKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSRNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASSSEMWSAGSKPLALCLCWRIRAFREDPFSASAVGNVAAAIEGDRRISEELWRWRIQVAVVGVSTQAVVRPQEESEAEGLLSSLLPRACLFQAMASLEPVPPPRAALVPVVLATELKETVAAELGQWQEMRQCFLRNLMRRSHCWSQFLSLVHLQCWKFLNEDLFAAAVAGVKASQ
Homology
BLAST of Sgr023327 vs. NCBI nr
Match: XP_038900958.1 (DNA ligase 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 1415.6 bits (3663), Expect = 0.0e+00
Identity = 725/781 (92.83%), Postives = 745/781 (95.39%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPPSFKPIFQNFVSAMTSRPSAFDALMSGARAA 131
           MLSLRSCYYL P+TLPHPQFKKLIF NKPPSFKPIFQNFVS+M+SRPSAFDALMSGARAA
Sbjct: 1   MLSLRSCYYLRPITLPHPQFKKLIFFNKPPSFKPIFQNFVSSMSSRPSAFDALMSGARAA 60

Query: 132 AKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNS--TLDNHGGSHLGTPE 191
           AKKK   SSPKKRKALDSLDPPPKPSSS  LESTP VESQCG+S   LDNHGG+HL   E
Sbjct: 61  AKKKPVNSSPKKRKALDSLDPPPKPSSSVALESTPAVESQCGSSIANLDNHGGTHLVKSE 120

Query: 192 NAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKNV 251
           +A DLGETKLRQDNGGSV  KN    NK CA+SVQERTAKLKS +PELK KAKDFNSKNV
Sbjct: 121 DAADLGETKLRQDNGGSVEPKNGPFGNKACASSVQERTAKLKSSIPELKRKAKDFNSKNV 180

Query: 252 ACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKI 311
           ACWKEGERVPFLFLCLGFDM+SEESSRI ITDIVCNMLRTVMDTTPDDLV+TVYLLANKI
Sbjct: 181 ACWKEGERVPFLFLCLGFDMISEESSRIIITDIVCNMLRTVMDTTPDDLVSTVYLLANKI 240

Query: 312 APAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPDA 371
           APAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPDA
Sbjct: 241 APAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPDA 300

Query: 372 LTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQT 431
           LTI KVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQT
Sbjct: 301 LTITKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQT 360

Query: 432 LLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLSK 491
           LLAALGQAAVYAEKHSTPP NIQSPLEEA+KIVKQVYSILPVYDKLVPALLSGGVWNLSK
Sbjct: 361 LLAALGQAAVYAEKHSTPPSNIQSPLEEASKIVKQVYSILPVYDKLVPALLSGGVWNLSK 420

Query: 492 TCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSRN 551
           TCNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSRN
Sbjct: 421 TCNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSRN 480

Query: 552 AERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVSD 611
           AERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYD GKQKILPFQILSTRARKNVA+SD
Sbjct: 481 AERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDHGKQKILPFQILSTRARKNVAMSD 540

Query: 612 IKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQKF 671
           IKV+VCVFAFDLLYLDGQPLIQRELNVRRELLYD F+EEPGYFQFATAIITNDLEEIQKF
Sbjct: 541 IKVEVCVFAFDLLYLDGQPLIQRELNVRRELLYDSFIEEPGYFQFATAIITNDLEEIQKF 600

Query: 672 LDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRGK 731
           LDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRGK
Sbjct: 601 LDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRGK 660

Query: 732 RTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDTI 791
           RTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIPEPKSYYRYGDTI
Sbjct: 661 RTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPEPKSYYRYGDTI 720

Query: 792 NPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASSS 845
           NPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASSS
Sbjct: 721 NPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASSS 780

BLAST of Sgr023327 vs. NCBI nr
Match: XP_022140452.1 (DNA ligase 1-like [Momordica charantia])

HSP 1 Score: 1414.8 bits (3661), Expect = 0.0e+00
Identity = 731/783 (93.36%), Postives = 746/783 (95.27%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVT--LPHPQFKKLIFSNKPPSFKPIFQNFVSAMTSRPSAFDALMSGAR 131
           MLSLRSCYYL PVT  LPHPQFKKLIF NK  SFKPI  NFVSAM+SRPSAFDALMSGAR
Sbjct: 1   MLSLRSCYYLRPVTIALPHPQFKKLIFFNKTHSFKPILPNFVSAMSSRPSAFDALMSGAR 60

Query: 132 AAAKKKSA--ASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNSTLDNHGGSHLGT 191
           AAAKKKSA   SSPKKRKALDSLDP PKPSSS TLES  PVESQCGNS LDN GGSHLGT
Sbjct: 61  AAAKKKSATSTSSPKKRKALDSLDPSPKPSSSHTLESMLPVESQCGNSILDNPGGSHLGT 120

Query: 192 PENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSK 251
            ENAVDLGETKL QDNGGSVGTKN  A +KTCATSVQERTAKLKS +PELK KAKDFNSK
Sbjct: 121 AENAVDLGETKLGQDNGGSVGTKNGPAGSKTCATSVQERTAKLKSSIPELKKKAKDFNSK 180

Query: 252 NVACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLAN 311
           NVACWKEGERVP LFLCLGFDM+SEESSRI ITDIVCNMLRTVMDTTPDDLVATVYLLAN
Sbjct: 181 NVACWKEGERVPCLFLCLGFDMISEESSRIVITDIVCNMLRTVMDTTPDDLVATVYLLAN 240

Query: 312 KIAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKP 371
           KIAPAHEGLELGIGD+SIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKP
Sbjct: 241 KIAPAHEGLELGIGDSSIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKP 300

Query: 372 DALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAE 431
           DALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAE
Sbjct: 301 DALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAE 360

Query: 432 QTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNL 491
           QTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAK VKQVYSILPVYDKLVPALL+GGVWNL
Sbjct: 361 QTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKTVKQVYSILPVYDKLVPALLTGGVWNL 420

Query: 492 SKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYS 551
           SKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHY+ENGSVEIYS
Sbjct: 421 SKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYLENGSVEIYS 480

Query: 552 RNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAV 611
           RNAERNTGKFPDVV+TVSRVKKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAV
Sbjct: 481 RNAERNTGKFPDVVLTVSRVKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAV 540

Query: 612 SDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQ 671
           SDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYD FMEEPGYFQFATAI+TNDLEEIQ
Sbjct: 541 SDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDSFMEEPGYFQFATAILTNDLEEIQ 600

Query: 672 KFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGR 731
           KFLDASVD+SCEGLIIKTLNRD+TYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGR
Sbjct: 601 KFLDASVDESCEGLIIKTLNRDSTYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGR 660

Query: 732 GKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGD 791
           GKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERS SLRSKVI EPKSYYRYGD
Sbjct: 661 GKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSTSLRSKVITEPKSYYRYGD 720

Query: 792 TINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEAS 845
           TINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVR+DK PEEAS
Sbjct: 721 TINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRQDKTPEEAS 780

BLAST of Sgr023327 vs. NCBI nr
Match: TYK24176.1 (DNA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1384.0 bits (3581), Expect = 0.0e+00
Identity = 716/782 (91.56%), Postives = 738/782 (94.37%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN P  SFKPI QN VS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNPSFSFKPISQNLVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNS--TLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLDPP KPSS   LESTPPVESQCG+S   LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKL QDNGG VG K     NKTCA+SVQERTAKLKS + ELK KAKDFNSKN
Sbjct: 121 -DVVDLGETKLGQDNGGGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPFLFLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFLFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVYAEKHSTPPPNIQSPLEEA+KIVKQV+SILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           K CNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KACNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELN RRELLYD F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNARRELLYDSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIP+PKSYYRYGD 
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPQPKSYYRYGDA 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPV+RAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVYRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 778

BLAST of Sgr023327 vs. NCBI nr
Match: KAA0037310.1 (DNA ligase 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 1380.9 bits (3573), Expect = 0.0e+00
Identity = 715/782 (91.43%), Postives = 737/782 (94.25%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN P  SFKPI QN VS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNPSFSFKPISQNLVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNS--TLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLDPP KPSS   LESTPPVESQCG+S   LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKL QDNGG VG K     NKTCA+SVQERTAKLKS + ELK KAKDFNSKN
Sbjct: 121 -DVVDLGETKLGQDNGGGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPFLFLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFLFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVYAEKHSTPPPNIQSPLEEA+KIVKQV+SILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           K CNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KACNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELN RRELLYD F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNARRELLYDSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIP+PKSYYRYGD 
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPQPKSYYRYGDA 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPV+RAGVGIVDSDKGISLRFPRLVRVRKDK PEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVYRAGVGIVDSDKGISLRFPRLVRVRKDKYPEEASS 778

BLAST of Sgr023327 vs. NCBI nr
Match: XP_004145818.1 (DNA ligase 1 [Cucumis sativus] >KGN46876.1 hypothetical protein Csa_021053 [Cucumis sativus])

HSP 1 Score: 1380.9 bits (3573), Expect = 0.0e+00
Identity = 715/782 (91.43%), Postives = 737/782 (94.25%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN    SFKPI  NFVS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNSSFSFKPISHNFVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGN--STLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLD P KPSS   LESTPPVESQCG+  S LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDSPLKPSSFLALESTPPVESQCGSSVSNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKLRQDN G VG K     NKTCA+SVQERTAKLKS   ELK KAKDFNSKN
Sbjct: 121 -DGVDLGETKLRQDNDGGVGAKIGPFGNKTCASSVQERTAKLKSSTAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPFLFLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFLFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALT+ KVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTVTKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVY+EKHSTPPPNIQSPLEEA+KIVKQVYSILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYSEKHSTPPPNIQSPLEEASKIVKQVYSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           KTCNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELNVRRELLY+ F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNVRRELLYNSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIPEPKSYYRYGDT
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPEPKSYYRYGDT 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVR+DKNPEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVREDKNPEEASS 778

BLAST of Sgr023327 vs. ExPASy Swiss-Prot
Match: Q42572 (DNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=LIG1 PE=2 SV=2)

HSP 1 Score: 994.2 bits (2569), Expect = 1.1e-288
Identity = 526/792 (66.41%), Postives = 621/792 (78.41%), Query Frame = 0

Query: 72  MLSLRSCYY------LCPVTLPHPQFKKLIFSNKPPSFKPIFQNFVSAMTSRPSAFDALM 131
           ML++RS  Y      LC  T        LI  ++  S   +     +  +SRPSAFDALM
Sbjct: 1   MLAIRSSNYLRCIPSLCTKTQISQFSSVLISFSRQISHLRLSSCHRAMSSSRPSAFDALM 60

Query: 132 SGARAAAKKK-----SAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNSTLDNH 191
           S ARAAAKKK     + + SP KRK  ++ D                  +  G + +   
Sbjct: 61  SNARAAAKKKTPQTTNLSRSPNKRKIGETQD------------------ANLGKTIVSE- 120

Query: 192 GGSHLGTPENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQ--ERTAKLKSCVPELK 251
                GT     DL E      N  S  T ++   +KT A   +   +T ++KS +  LK
Sbjct: 121 -----GTLPKTEDLLEPVSDSANPRS-DTSSIAEDSKTGAKKAKTLSKTDEMKSKIGLLK 180

Query: 252 MKAKDFNSKNVACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDL 311
            K  DF+ + ++CW++GERVPFLF+ L FD++S ES RI ITDI+CNMLRTV+ TTP+DL
Sbjct: 181 KKPNDFDPEKMSCWEKGERVPFLFVALAFDLISNESGRIVITDILCNMLRTVIATTPEDL 240

Query: 312 VATVYLLANKIAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASR 371
           VATVYL AN+IAPAHEG+ELGIG+++IIKA+SEAFGRTE        ELGDLGLVAK SR
Sbjct: 241 VATVYLSANEIAPAHEGVELGIGESTIIKAISEAFGRTEDHVKKQNTELGDLGLVAKGSR 300

Query: 372 SSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQ 431
           S+Q+MM KP+ LT+ KVFDTFR IAKESGKDS EKKKN +K+LLVA TDCEPLYL RLLQ
Sbjct: 301 STQTMMFKPEPLTVVKVFDTFRQIAKESGKDSNEKKKNRMKALLVATTDCEPLYLTRLLQ 360

Query: 432 TKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPA 491
            KLR+G + QT+LAALGQAAVY E+HS PPPN +SPLEEAAKIVKQV+++LPVYD +VPA
Sbjct: 361 AKLRLGFSGQTVLAALGQAAVYNEEHSKPPPNTKSPLEEAAKIVKQVFTVLPVYDIIVPA 420

Query: 492 LLSGGVWNLSKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYM 551
           LLSGGVWNL KTCNFT G+PIGPMLAKPTKGV+EILNKFQD+ FTCEYKYDGERAQIH+M
Sbjct: 421 LLSGGVWNLPKTCNFTLGVPIGPMLAKPTKGVAEILNKFQDIVFTCEYKYDGERAQIHFM 480

Query: 552 ENGSVEIYSRNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILS 611
           E+G+ EIYSRNAERNTGK+PDV + +SR+KKPSVKSFILDCE+VA+DR K+KILPFQILS
Sbjct: 481 EDGTFEIYSRNAERNTGKYPDVALALSRLKKPSVKSFILDCEVVAFDREKKKILPFQILS 540

Query: 612 TRARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAI 671
           TRARKNV V+DIKV VC+FAFD+LYL+GQ LIQ  L +RRE LY+ F E+PGYFQFATA+
Sbjct: 541 TRARKNVNVNDIKVGVCIFAFDMLYLNGQQLIQENLKIRREKLYESFEEDPGYFQFATAV 600

Query: 672 ITNDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDL 731
            +ND++EIQKFLDASVD  CEGLIIKTL+ DATYEP+KRS+NWLKLKKDY+D+IGDS+DL
Sbjct: 601 TSNDIDEIQKFLDASVDVGCEGLIIKTLDSDATYEPAKRSNNWLKLKKDYMDSIGDSVDL 660

Query: 732 VPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPE 791
           VPIAAFHGRGKRTGVYGAFLLACYD D EEFQSICKIGTGFS+A+L+ERS+SLRS+VI  
Sbjct: 661 VPIAAFHGRGKRTGVYGAFLLACYDVDKEEFQSICKIGTGFSDAMLDERSSSLRSQVIAT 720

Query: 792 PKSYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVR 845
           PK YYR GD++NPDVWFEPTEVWEVKAADLTISPVHRA  GIVD DKGISLRFPRL+RVR
Sbjct: 721 PKQYYRVGDSLNPDVWFEPTEVWEVKAADLTISPVHRAATGIVDPDKGISLRFPRLLRVR 767

BLAST of Sgr023327 vs. ExPASy Swiss-Prot
Match: P51892 (DNA ligase 1 OS=Xenopus laevis OX=8355 GN=lig1 PE=2 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 7.8e-178
Identity = 336/729 (46.09%), Postives = 463/729 (63.51%), Query Frame = 0

Query: 133  KKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNSTLDNHGGSHLGTPENAV 192
            K     S  + +K  DS   P K  SS      P ++++     L            N  
Sbjct: 338  KSNEEKSDEEPQKKADSA-KPVKQISSFFAPRKPAIKTEKREENL------------NEK 397

Query: 193  DLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKNVACW 252
            ++ ET L +         +   A+K  AT  Q      KS           ++  + ACW
Sbjct: 398  NVTETSLEESPKPKKNVGSFFGASKQEATEEQTEYNPSKS----------KYHPIDDACW 457

Query: 253  KEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPA 312
              G++VP+L +   F+ + EES+R+   + + N LR+V+  TP+DL+  +YL  N++ PA
Sbjct: 458  CNGQKVPYLAVARTFERIEEESARLKNVETLSNFLRSVIALTPEDLLPCIYLCLNRLGPA 517

Query: 313  HEGLELGIGDASIIKALSEAFGR------TEAQELGDLGLVAKASRSSQSMMRKPDALTI 372
            +EGLELGIG+  ++KA+++A GR       EAQE GDLGLVA++SRS+Q  M  P  L  
Sbjct: 518  YEGLELGIGETILMKAVAQATGRQLEKIKAEAQEKGDLGLVAESSRSNQRTMFTPPKLMA 577

Query: 373  AKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQTLLA 432
            + VF   + IA+ +G  S  KK + IK L VA    E  Y+ R L  KLRIGLAEQ++L+
Sbjct: 578  SGVFGKLKDIARMTGNASMNKKIDIIKGLFVACRHSEARYIARSLGGKLRIGLAEQSVLS 637

Query: 433  ALGQAAVYAEKHSTPPPNI------------QSPLEEAAKIVKQVYSILPVYDKLVPALL 492
            ++ QA          PP +            +S +EE A I+KQ +  LP YD ++P LL
Sbjct: 638  SIAQAVCLTPPGRDAPPTVMDAGKGMSADARKSWIEEKAMILKQTFCELPNYDAIIPILL 697

Query: 493  SGGVWNLSKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMEN 552
              G+ +L K C  TPGIP+ PMLA PTKG+ E+L +F +  FTCEYKYDGERAQIH +EN
Sbjct: 698  EHGIDDLPKHCRLTPGIPLKPMLAHPTKGIGEVLKRFDEAAFTCEYKYDGERAQIHILEN 757

Query: 553  GSVEIYSRNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTR 612
            G V +YSRN E NT K+PD++  + ++KK SVKS ILD E VA D  K++I PFQ+L+TR
Sbjct: 758  GEVHVYSRNQENNTTKYPDIISRIPKIKKESVKSCILDTEAVAGDAEKKQIQPFQVLTTR 817

Query: 613  ARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIIT 672
             RK+V  S+IKV VCV+AFD+LYL+G+ L++     RR+LL D F+E  G F FAT +  
Sbjct: 818  KRKDVDASEIKVQVCVYAFDMLYLNGESLVKEPFAKRRQLLRDSFLETEGQFMFATYMDK 877

Query: 673  NDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVP 732
            ++ +EI +FLD S+  SCEGL++KTL +DATYE +KRSHNWLKLKKDY++ +GD+LDLV 
Sbjct: 878  SNTDEISEFLDQSIKDSCEGLMVKTLEQDATYEIAKRSHNWLKLKKDYLEGVGDTLDLVV 937

Query: 733  IAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPK 792
            I A+ G+GKRTG+YG FLLA YD ++EE+Q+ICKIGTGF++  LE+    L+  VI  P+
Sbjct: 938  IGAYLGKGKRTGIYGGFLLASYDEESEEYQTICKIGTGFTDDDLEKHYNFLKDHVIENPR 997

Query: 793  SYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKD 844
            SYYR+     PD WF+P +VWEVK ADL+ISPVH+A +G+V+  KGISLRFPR +R+R D
Sbjct: 998  SYYRWDSATEPDHWFDPVQVWEVKCADLSISPVHKAALGLVEDQKGISLRFPRFLRIRDD 1043

BLAST of Sgr023327 vs. ExPASy Swiss-Prot
Match: Q869E1 (DNA ligase 1 OS=Dictyostelium discoideum OX=44689 GN=lig1 PE=3 SV=1)

HSP 1 Score: 625.5 bits (1612), Expect = 1.0e-177
Identity = 317/641 (49.45%), Postives = 445/641 (69.42%), Query Frame = 0

Query: 223  VQERTAKLKSCVPELKMKAKDFNSKNVACWKEGERVPFLFLCLGFDMVSEESSRITITDI 282
            + ++    K+   +LK+  K +     A WK+GE VP++ L   F+M+   SSR+ I + 
Sbjct: 528  ISKKKGNAKTIQADLKVIGK-YRPIEDAQWKKGEAVPYMVLAKTFEMMESTSSRLIIIEH 587

Query: 283  VCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGIGDASIIKALSEAFGRT------ 342
            + N+ R++M  +P DLV T+YL  NKI P+++  ELGIG+  +IK+L+E+ GR+      
Sbjct: 588  LANLFRSIMLLSPKDLVMTIYLSINKIGPSYQSKELGIGEHVLIKSLAESTGRSVDVIKQ 647

Query: 343  EAQELGDLGLVAKASRSSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLL 402
            E  E+GDLG++A+ SRS+Q++M KP  LTI  VF TF+ IA  SG   Q+KKK+ IK LL
Sbjct: 648  ELTEVGDLGIIAQNSRSTQTLMGKPTPLTIQSVFKTFQQIADLSGTGGQQKKKDLIKKLL 707

Query: 403  VAATDCEPLYLIRLLQTKLRIGLAEQTLLAALGQAA------------VYAEKHSTPPPN 462
            V+  DCE LY+IR LQ KLRIGLAE+++L AL ++             ++  +       
Sbjct: 708  VSCKDCETLYIIRSLQGKLRIGLAERSVLMALAKSVLVTPPIDGSGQQIFDIRKQMKQEE 767

Query: 463  IQSPLEEAAKIVKQVYSILPVYDKLVPALLS-GGVWNLSKTCNFTPGIPIGPMLAKPTKG 522
             +   +     V + YS LP YD  VP L++  G+ N+  TC+   GIP+ PMLA+PT G
Sbjct: 768  FEERYQNVVSKVTRAYSQLPNYDLFVPHLIAVNGIDNILSTCSLKVGIPVKPMLAQPTTG 827

Query: 523  VSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSRNAERNTGKFPDVVITVSRVKK 582
            +S++L++F D+EFTCE+KYDGERAQIH + +G+  IY+RN E  T K+PD+V  V++   
Sbjct: 828  ISQMLDRFSDMEFTCEFKYDGERAQIHRLPDGTTHIYTRNLEDYTQKYPDIVANVTKFVG 887

Query: 583  PSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVSDIKVDVCVFAFDLLYLDGQPL 642
            P+VKSFILDCE VA+D   +KIL FQ+LSTRARK+V +S IKV VCVFAFDLLYL+GQ L
Sbjct: 888  PNVKSFILDCEAVAFDAATKKILSFQVLSTRARKSVQLSQIKVPVCVFAFDLLYLNGQSL 947

Query: 643  IQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQKFLDASVDQSCEGLIIKTLNRD 702
            I   L  RRE L + F+   G F FA     +D+ +IQ +L+ +V+ +CEGL++KTL   
Sbjct: 948  IDEPLIKRREHLVENFIASDGVFAFAKYSNISDVNDIQSYLEEAVEGNCEGLMVKTLKEK 1007

Query: 703  ATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRGKRTGVYGAFLLACYDNDNEEF 762
            + YEPS+RS+NWLK+KKDY+  + DSLDLVPI A++G+GKRTG YGA+LLACYD +NEEF
Sbjct: 1008 SIYEPSRRSYNWLKIKKDYMQGMTDSLDLVPIGAWYGKGKRTGTYGAYLLACYDENNEEF 1067

Query: 763  QSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDTINPDVWFEPTEVWEVKAADLT 822
            Q++CKIGTGFS+  L   ++ L+  +I +P++ +R+ D+I PDVWF P+ VWEV AADL+
Sbjct: 1068 QTLCKIGTGFSDEQLTTFTSQLKPHLINQPRNQFRFSDSIKPDVWFSPSCVWEVLAADLS 1127

Query: 823  ISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASSSE 845
            ISPVH A  GI+D +KGI+LRFPR +R+R DK+PE+A+SS+
Sbjct: 1128 ISPVHTAASGILDPNKGIALRFPRFIRIRPDKSPEDATSSD 1167

BLAST of Sgr023327 vs. ExPASy Swiss-Prot
Match: P18858 (DNA ligase 1 OS=Homo sapiens OX=9606 GN=LIG1 PE=1 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 6.6e-177
Identity = 309/612 (50.49%), Postives = 430/612 (70.26%), Query Frame = 0

Query: 250 ACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKI 309
           ACWK G++VP+L +   F+ + E S+R+ + + + N+LR+V+  +P DL+  +YL  N +
Sbjct: 279 ACWKPGQKVPYLAVARTFEKIEEVSARLRMVETLSNLLRSVVALSPPDLLPVLYLSLNHL 338

Query: 310 APAHEGLELGIGDASIIKALSEAFG------RTEAQELGDLGLVAKASRSSQSMMRKPDA 369
            P  +GLELG+GD  ++KA+++A G      R EA E GD+GLVA+ SRS+Q +M  P  
Sbjct: 339 GPPQQGLELGVGDGVLLKAVAQATGRQLESVRAEAAEKGDVGLVAENSRSTQRLMLPPPP 398

Query: 370 LTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQT 429
           LT + VF  FR IA+ +G  S  KK + IK L VA    E  ++ R L  +LR+GLAEQ+
Sbjct: 399 LTASGVFSKFRDIARLTGSASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQS 458

Query: 430 LLAALGQAAVYAEKHSTPPPNI------------QSPLEEAAKIVKQVYSILPVYDKLVP 489
           +LAAL QA          PP +            ++ LEE   I+KQ +  +P  D+++P
Sbjct: 459 VLAALSQAVSLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRIIP 518

Query: 490 ALLSGGVWNLSKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHY 549
            LL  G+  L + C  +PGIP+ PMLA PT+G+SE+L +F++  FTCEYKYDG+RAQIH 
Sbjct: 519 VLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEYKYDGQRAQIHA 578

Query: 550 MENGSVEIYSRNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQIL 609
           +E G V+I+SRN E NTGK+PD++  + ++K PSV SFILD E VA+DR K++I PFQ+L
Sbjct: 579 LEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFILDTEAVAWDREKKQIQPFQVL 638

Query: 610 STRARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATA 669
           +TR RK V  S+I+V VC++AFDL+YL+G+ L++  L+ RR+LL + F+E  G F FAT+
Sbjct: 639 TTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVREPLSRRRQLLRENFVETEGEFVFATS 698

Query: 670 IITNDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLD 729
           + T D+E+I +FL+ SV  SCEGL++KTL+ DATYE +KRSHNWLKLKKDY+D +GD+LD
Sbjct: 699 LDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLD 758

Query: 730 LVPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIP 789
           LV I A+ GRGKR G YG FLLA YD D+EE Q+ICK+GTGFS+  LEE   SL++ V+P
Sbjct: 759 LVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLP 818

Query: 790 EPKSYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRV 844
            P+ Y R    + PD W +P+ VWEVK ADL++SP++ A  G+VDSDKGISLRFPR +RV
Sbjct: 819 SPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRV 878

BLAST of Sgr023327 vs. ExPASy Swiss-Prot
Match: P37913 (DNA ligase 1 OS=Mus musculus OX=10090 GN=Lig1 PE=1 SV=2)

HSP 1 Score: 620.9 bits (1600), Expect = 2.5e-176
Identity = 309/618 (50.00%), Postives = 430/618 (69.58%), Query Frame = 0

Query: 250 ACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKI 309
           ACWK G++VPFL +   F+ + E S+R+ + + + N+LR+V+  +P DL+  +YL  N++
Sbjct: 277 ACWKHGQKVPFLAVARTFEKIEEVSARLKMVETLSNLLRSVVALSPPDLLPVLYLSLNRL 336

Query: 310 APAHEGLELGIGDASIIKALSEAFG------RTEAQELGDLGLVAKASRSSQSMMRKPDA 369
            P  +GLELG+GD  ++KA+++A G      R E  E GD+GLVA+ SRS+Q +M  P  
Sbjct: 337 GPPQQGLELGVGDGVLLKAVAQATGRQLESIRAEVAEKGDVGLVAENSRSTQRLMLPPPP 396

Query: 370 LTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQT 429
           LTI+ VF  F  IA+ +G  S  KK + IK L VA    E  Y+ R L  +LR+GLAEQ+
Sbjct: 397 LTISGVFTKFCDIARLTGSASMAKKMDIIKGLFVACRHSEARYIARSLSGRLRLGLAEQS 456

Query: 430 LLAALGQAAVYAEKHSTPPPNIQSP------------------LEEAAKIVKQVYSILPV 489
           +LAAL QA       S  PP  + P                  LEE   I+KQ +  +P 
Sbjct: 457 VLAALAQAV------SLTPPGQEFPTVVVDAGKGKTAEARKMWLEEQGMILKQTFCEVPD 516

Query: 490 YDKLVPALLSGGVWNLSKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGE 549
            D+++P LL  G+  L + C  +PG+P+ PMLA PT+GVSE+L +F++++FTCEYKYDG+
Sbjct: 517 LDRIIPVLLEHGLERLPEHCKLSPGVPLKPMLAHPTRGVSEVLKRFEEVDFTCEYKYDGQ 576

Query: 550 RAQIHYMENGSVEIYSRNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKI 609
           RAQIH +E G V+I+SRN E NTGK+PD++  + ++K PSV SFILD E VA+DR K++I
Sbjct: 577 RAQIHVLEGGEVKIFSRNQEDNTGKYPDIISRIPKIKHPSVTSFILDTEAVAWDREKKQI 636

Query: 610 LPFQILSTRARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGY 669
            PFQ+L+TR RK V  S+I+V VC++AFDL+YL+G+ L+++ L+ RR+LL + F+E  G 
Sbjct: 637 QPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNGESLVRQPLSRRRQLLRENFVETEGE 696

Query: 670 FQFATAIITNDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDN 729
           F F T++ T D E+I +FL+ SV  SCEGL++KTL+ DATYE +KRSHNWLKLKKDY+D 
Sbjct: 697 FVFTTSLDTKDTEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDG 756

Query: 730 IGDSLDLVPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASL 789
           +GD+LDLV I A+ GRGKR G YG FLLA YD ++EE Q+ICK+GTGFS+  LEE   SL
Sbjct: 757 VGDTLDLVVIGAYLGRGKRAGRYGGFLLAAYDEESEELQAICKLGTGFSDEELEEHHQSL 816

Query: 790 RSKVIPEPKSYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRF 844
           ++ V+P P+ Y R    + PD W +P+ VWEVK ADL++SP++ A  G+VD +KGISLRF
Sbjct: 817 QALVLPTPRPYVRIDGAVAPDHWLDPSIVWEVKCADLSLSPIYPAARGLVDKEKGISLRF 876

BLAST of Sgr023327 vs. ExPASy TrEMBL
Match: A0A6J1CGY4 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111011122 PE=3 SV=1)

HSP 1 Score: 1414.8 bits (3661), Expect = 0.0e+00
Identity = 731/783 (93.36%), Postives = 746/783 (95.27%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVT--LPHPQFKKLIFSNKPPSFKPIFQNFVSAMTSRPSAFDALMSGAR 131
           MLSLRSCYYL PVT  LPHPQFKKLIF NK  SFKPI  NFVSAM+SRPSAFDALMSGAR
Sbjct: 1   MLSLRSCYYLRPVTIALPHPQFKKLIFFNKTHSFKPILPNFVSAMSSRPSAFDALMSGAR 60

Query: 132 AAAKKKSA--ASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNSTLDNHGGSHLGT 191
           AAAKKKSA   SSPKKRKALDSLDP PKPSSS TLES  PVESQCGNS LDN GGSHLGT
Sbjct: 61  AAAKKKSATSTSSPKKRKALDSLDPSPKPSSSHTLESMLPVESQCGNSILDNPGGSHLGT 120

Query: 192 PENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSK 251
            ENAVDLGETKL QDNGGSVGTKN  A +KTCATSVQERTAKLKS +PELK KAKDFNSK
Sbjct: 121 AENAVDLGETKLGQDNGGSVGTKNGPAGSKTCATSVQERTAKLKSSIPELKKKAKDFNSK 180

Query: 252 NVACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLAN 311
           NVACWKEGERVP LFLCLGFDM+SEESSRI ITDIVCNMLRTVMDTTPDDLVATVYLLAN
Sbjct: 181 NVACWKEGERVPCLFLCLGFDMISEESSRIVITDIVCNMLRTVMDTTPDDLVATVYLLAN 240

Query: 312 KIAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKP 371
           KIAPAHEGLELGIGD+SIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKP
Sbjct: 241 KIAPAHEGLELGIGDSSIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKP 300

Query: 372 DALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAE 431
           DALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAE
Sbjct: 301 DALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAE 360

Query: 432 QTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNL 491
           QTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAK VKQVYSILPVYDKLVPALL+GGVWNL
Sbjct: 361 QTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKTVKQVYSILPVYDKLVPALLTGGVWNL 420

Query: 492 SKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYS 551
           SKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHY+ENGSVEIYS
Sbjct: 421 SKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYLENGSVEIYS 480

Query: 552 RNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAV 611
           RNAERNTGKFPDVV+TVSRVKKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAV
Sbjct: 481 RNAERNTGKFPDVVLTVSRVKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAV 540

Query: 612 SDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQ 671
           SDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYD FMEEPGYFQFATAI+TNDLEEIQ
Sbjct: 541 SDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDSFMEEPGYFQFATAILTNDLEEIQ 600

Query: 672 KFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGR 731
           KFLDASVD+SCEGLIIKTLNRD+TYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGR
Sbjct: 601 KFLDASVDESCEGLIIKTLNRDSTYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGR 660

Query: 732 GKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGD 791
           GKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERS SLRSKVI EPKSYYRYGD
Sbjct: 661 GKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSTSLRSKVITEPKSYYRYGD 720

Query: 792 TINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEAS 845
           TINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVR+DK PEEAS
Sbjct: 721 TINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRQDKTPEEAS 780

BLAST of Sgr023327 vs. ExPASy TrEMBL
Match: A0A5D3DKM3 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1754G00060 PE=3 SV=1)

HSP 1 Score: 1384.0 bits (3581), Expect = 0.0e+00
Identity = 716/782 (91.56%), Postives = 738/782 (94.37%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN P  SFKPI QN VS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNPSFSFKPISQNLVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNS--TLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLDPP KPSS   LESTPPVESQCG+S   LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKL QDNGG VG K     NKTCA+SVQERTAKLKS + ELK KAKDFNSKN
Sbjct: 121 -DVVDLGETKLGQDNGGGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPFLFLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFLFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVYAEKHSTPPPNIQSPLEEA+KIVKQV+SILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           K CNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KACNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELN RRELLYD F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNARRELLYDSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIP+PKSYYRYGD 
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPQPKSYYRYGDA 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPV+RAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVYRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 778

BLAST of Sgr023327 vs. ExPASy TrEMBL
Match: A0A5A7T1R1 (DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3G00220 PE=3 SV=1)

HSP 1 Score: 1380.9 bits (3573), Expect = 0.0e+00
Identity = 715/782 (91.43%), Postives = 737/782 (94.25%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN P  SFKPI QN VS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNPSFSFKPISQNLVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNS--TLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLDPP KPSS   LESTPPVESQCG+S   LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKL QDNGG VG K     NKTCA+SVQERTAKLKS + ELK KAKDFNSKN
Sbjct: 121 -DVVDLGETKLGQDNGGGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPFLFLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFLFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVYAEKHSTPPPNIQSPLEEA+KIVKQV+SILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           K CNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KACNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELN RRELLYD F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNARRELLYDSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIP+PKSYYRYGD 
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPQPKSYYRYGDA 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPV+RAGVGIVDSDKGISLRFPRLVRVRKDK PEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVYRAGVGIVDSDKGISLRFPRLVRVRKDKYPEEASS 778

BLAST of Sgr023327 vs. ExPASy TrEMBL
Match: A0A0A0KGJ2 (DNA ligase OS=Cucumis sativus OX=3659 GN=Csa_6G148180 PE=3 SV=1)

HSP 1 Score: 1380.9 bits (3573), Expect = 0.0e+00
Identity = 715/782 (91.43%), Postives = 737/782 (94.25%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN    SFKPI  NFVS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNSSFSFKPISHNFVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGN--STLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLD P KPSS   LESTPPVESQCG+  S LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDSPLKPSSFLALESTPPVESQCGSSVSNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKLRQDN G VG K     NKTCA+SVQERTAKLKS   ELK KAKDFNSKN
Sbjct: 121 -DGVDLGETKLRQDNDGGVGAKIGPFGNKTCASSVQERTAKLKSSTAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPFLFLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFLFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALT+ KVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTVTKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVY+EKHSTPPPNIQSPLEEA+KIVKQVYSILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYSEKHSTPPPNIQSPLEEASKIVKQVYSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           KTCNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELNVRRELLY+ F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNVRRELLYNSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIPEPKSYYRYGDT
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPEPKSYYRYGDT 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVR+DKNPEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVREDKNPEEASS 778

BLAST of Sgr023327 vs. ExPASy TrEMBL
Match: A0A1S3CNQ9 (DNA ligase OS=Cucumis melo OX=3656 GN=LOC103503033 PE=3 SV=1)

HSP 1 Score: 1379.0 bits (3568), Expect = 0.0e+00
Identity = 714/782 (91.30%), Postives = 736/782 (94.12%), Query Frame = 0

Query: 72  MLSLRSCYYLCPVTLPHPQFKKLIFSNKPP-SFKPIFQNFVSAMTSRPSAFDALMSGARA 131
           MLSLRSC+YL P+TLPHPQFKKLIFSN P  SFKPI QN VS M+SRPSAFDALMSGARA
Sbjct: 1   MLSLRSCFYLRPITLPHPQFKKLIFSNNPSFSFKPISQNLVSPMSSRPSAFDALMSGARA 60

Query: 132 AAKKKSAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNS--TLDNHGGSHLGTP 191
           AAKKK A SSPKKRKALDSLDPP KPSS   LESTPPVESQCG+S   LD+HGG+HL   
Sbjct: 61  AAKKKPANSSPKKRKALDSLDPPLKPSSVLALESTPPVESQCGSSIPNLDDHGGTHL--- 120

Query: 192 ENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKN 251
            + VDLGETKL QDNGG VG K     NKTCA+SVQERTAKLKS + ELK KAKDFNSKN
Sbjct: 121 -DVVDLGETKLGQDNGGGVGAKIGPFGNKTCASSVQERTAKLKSSIAELKKKAKDFNSKN 180

Query: 252 VACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 311
           VACWKEGERVPF FLCLGFDM+SEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK
Sbjct: 181 VACWKEGERVPFXFLCLGFDMISEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANK 240

Query: 312 IAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPD 371
           IAPAHEGLELGIGDASIIKALSEAFGRTEAQ      ELGDLGLVAKASRSSQSMMRKPD
Sbjct: 241 IAPAHEGLELGIGDASIIKALSEAFGRTEAQVKKQYKELGDLGLVAKASRSSQSMMRKPD 300

Query: 372 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 431
           ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ
Sbjct: 301 ALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQ 360

Query: 432 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 491
           TLLAALGQAAVYAEKHSTPPPNIQSPLEEA+KIVKQV+SILPVYDKLVPALLSGGVWNLS
Sbjct: 361 TLLAALGQAAVYAEKHSTPPPNIQSPLEEASKIVKQVFSILPVYDKLVPALLSGGVWNLS 420

Query: 492 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSR 551
           K CNFTPGIPIGPMLAKPTKGVSEILNKFQ LEFTCEYKYDGERAQIHYME+GSVEIYSR
Sbjct: 421 KACNFTPGIPIGPMLAKPTKGVSEILNKFQGLEFTCEYKYDGERAQIHYMEDGSVEIYSR 480

Query: 552 NAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 611
           NAERNTGKFPDVV+TVSR+KKPSV+SFILDCELVAYDRGKQKILPFQILSTRARKNVAVS
Sbjct: 481 NAERNTGKFPDVVLTVSRIKKPSVRSFILDCELVAYDRGKQKILPFQILSTRARKNVAVS 540

Query: 612 DIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQK 671
           DIKV+VCVFAFDLLYLDGQPLIQRELN RRELLYD F+EE GYFQFATAIITNDLEEIQK
Sbjct: 541 DIKVEVCVFAFDLLYLDGQPLIQRELNARRELLYDSFIEESGYFQFATAIITNDLEEIQK 600

Query: 672 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRG 731
           FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYID+IGDSLDLVPIAAFHGRG
Sbjct: 601 FLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDSIGDSLDLVPIAAFHGRG 660

Query: 732 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDT 791
           KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA+LEERSASLRSKVIP+PKSYYRYGD 
Sbjct: 661 KRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAMLEERSASLRSKVIPQPKSYYRYGDA 720

Query: 792 INPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASS 845
           INPDVWFEPTEVWEVKAADLTISPV+RAGVGIVDSDKGISLRFPRLVRVRKDK PEEASS
Sbjct: 721 INPDVWFEPTEVWEVKAADLTISPVYRAGVGIVDSDKGISLRFPRLVRVRKDKYPEEASS 778

BLAST of Sgr023327 vs. TAIR 10
Match: AT1G08130.1 (DNA ligase 1 )

HSP 1 Score: 994.2 bits (2569), Expect = 7.7e-290
Identity = 526/792 (66.41%), Postives = 621/792 (78.41%), Query Frame = 0

Query: 72  MLSLRSCYY------LCPVTLPHPQFKKLIFSNKPPSFKPIFQNFVSAMTSRPSAFDALM 131
           ML++RS  Y      LC  T        LI  ++  S   +     +  +SRPSAFDALM
Sbjct: 1   MLAIRSSNYLRCIPSLCTKTQISQFSSVLISFSRQISHLRLSSCHRAMSSSRPSAFDALM 60

Query: 132 SGARAAAKKK-----SAASSPKKRKALDSLDPPPKPSSSQTLESTPPVESQCGNSTLDNH 191
           S ARAAAKKK     + + SP KRK  ++ D                  +  G + +   
Sbjct: 61  SNARAAAKKKTPQTTNLSRSPNKRKIGETQD------------------ANLGKTIVSE- 120

Query: 192 GGSHLGTPENAVDLGETKLRQDNGGSVGTKNVLAANKTCATSVQ--ERTAKLKSCVPELK 251
                GT     DL E      N  S  T ++   +KT A   +   +T ++KS +  LK
Sbjct: 121 -----GTLPKTEDLLEPVSDSANPRS-DTSSIAEDSKTGAKKAKTLSKTDEMKSKIGLLK 180

Query: 252 MKAKDFNSKNVACWKEGERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDL 311
            K  DF+ + ++CW++GERVPFLF+ L FD++S ES RI ITDI+CNMLRTV+ TTP+DL
Sbjct: 181 KKPNDFDPEKMSCWEKGERVPFLFVALAFDLISNESGRIVITDILCNMLRTVIATTPEDL 240

Query: 312 VATVYLLANKIAPAHEGLELGIGDASIIKALSEAFGRTEAQ------ELGDLGLVAKASR 371
           VATVYL AN+IAPAHEG+ELGIG+++IIKA+SEAFGRTE        ELGDLGLVAK SR
Sbjct: 241 VATVYLSANEIAPAHEGVELGIGESTIIKAISEAFGRTEDHVKKQNTELGDLGLVAKGSR 300

Query: 372 SSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQ 431
           S+Q+MM KP+ LT+ KVFDTFR IAKESGKDS EKKKN +K+LLVA TDCEPLYL RLLQ
Sbjct: 301 STQTMMFKPEPLTVVKVFDTFRQIAKESGKDSNEKKKNRMKALLVATTDCEPLYLTRLLQ 360

Query: 432 TKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPA 491
            KLR+G + QT+LAALGQAAVY E+HS PPPN +SPLEEAAKIVKQV+++LPVYD +VPA
Sbjct: 361 AKLRLGFSGQTVLAALGQAAVYNEEHSKPPPNTKSPLEEAAKIVKQVFTVLPVYDIIVPA 420

Query: 492 LLSGGVWNLSKTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYM 551
           LLSGGVWNL KTCNFT G+PIGPMLAKPTKGV+EILNKFQD+ FTCEYKYDGERAQIH+M
Sbjct: 421 LLSGGVWNLPKTCNFTLGVPIGPMLAKPTKGVAEILNKFQDIVFTCEYKYDGERAQIHFM 480

Query: 552 ENGSVEIYSRNAERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPFQILS 611
           E+G+ EIYSRNAERNTGK+PDV + +SR+KKPSVKSFILDCE+VA+DR K+KILPFQILS
Sbjct: 481 EDGTFEIYSRNAERNTGKYPDVALALSRLKKPSVKSFILDCEVVAFDREKKKILPFQILS 540

Query: 612 TRARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQFATAI 671
           TRARKNV V+DIKV VC+FAFD+LYL+GQ LIQ  L +RRE LY+ F E+PGYFQFATA+
Sbjct: 541 TRARKNVNVNDIKVGVCIFAFDMLYLNGQQLIQENLKIRREKLYESFEEDPGYFQFATAV 600

Query: 672 ITNDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGDSLDL 731
            +ND++EIQKFLDASVD  CEGLIIKTL+ DATYEP+KRS+NWLKLKKDY+D+IGDS+DL
Sbjct: 601 TSNDIDEIQKFLDASVDVGCEGLIIKTLDSDATYEPAKRSNNWLKLKKDYMDSIGDSVDL 660

Query: 732 VPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEAVLEERSASLRSKVIPE 791
           VPIAAFHGRGKRTGVYGAFLLACYD D EEFQSICKIGTGFS+A+L+ERS+SLRS+VI  
Sbjct: 661 VPIAAFHGRGKRTGVYGAFLLACYDVDKEEFQSICKIGTGFSDAMLDERSSSLRSQVIAT 720

Query: 792 PKSYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPRLVRVR 845
           PK YYR GD++NPDVWFEPTEVWEVKAADLTISPVHRA  GIVD DKGISLRFPRL+RVR
Sbjct: 721 PKQYYRVGDSLNPDVWFEPTEVWEVKAADLTISPVHRAATGIVDPDKGISLRFPRLLRVR 767

BLAST of Sgr023327 vs. TAIR 10
Match: AT1G49250.1 (ATP-dependent DNA ligase )

HSP 1 Score: 803.5 bits (2074), Expect = 1.9e-232
Identity = 402/579 (69.43%), Postives = 483/579 (83.42%), Query Frame = 0

Query: 273 ESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEGLELGIGDAS-IIKALSE 332
           ES RI IT I+CNMLRTV+ TTPDDL+ TVYL AN+IAPAHEG++LG+G  S IIKA+SE
Sbjct: 58  ESGRIVITHILCNMLRTVIATTPDDLLPTVYLAANEIAPAHEGIKLGMGKGSYIIKAISE 117

Query: 333 AFGRTEAQ------ELGDLGLVAKASRSSQSMMRKPDALTIAKVFDTFRLIAKESGKDSQ 392
           AFGRTE+       +LGDLGLVA  SRSSQ+M+  P  LT+ KV DT RLIAKESGK S+
Sbjct: 118 AFGRTESHVEQQYTQLGDLGLVANGSRSSQTMIFMPKPLTVVKVADTLRLIAKESGKGSK 177

Query: 393 EKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQTLLAALGQAAVYAEKHSTPPPNI 452
           +KKK+ +K+LLVA TDCEPLYL RLLQ  LR+G + QT+LAALGQAAVY E+HS PPPNI
Sbjct: 178 DKKKDLMKALLVATTDCEPLYLTRLLQDNLRLGFSRQTVLAALGQAAVYNEEHSKPPPNI 237

Query: 453 QSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLSKTCNFTPGIPIGPMLAKPTKGVS 512
           ++PL+EAA IVK+V+S+LPVYD +V ALL+ GVWNL KTCN T G+P+ PMLAK T  V 
Sbjct: 238 KNPLDEAATIVKEVFSMLPVYDIIVGALLTSGVWNLPKTCNLTLGVPVRPMLAKATTRVD 297

Query: 513 EILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSRNAERNTGKFPDVVITVSRVKKPS 572
            IL KF+D  FT EYKYDGERAQI+YME+G+VEI+SR+AERNTGK+PDV + +SR+KKP+
Sbjct: 298 LILEKFKDTVFTAEYKYDGERAQIYYMEDGTVEIFSRHAERNTGKYPDVALVLSRLKKPT 357

Query: 573 VKSFILDCELVAYDRGKQKILPFQILSTRARKNVAVSDIKVDVCVFAFDLLYLDGQPLIQ 632
           VKSFILDCE+V ++R K+KILP Q  STRA KNV VSDIKV VCVFAFD+LYL+GQ LI 
Sbjct: 358 VKSFILDCEVVTFNREKEKILPLQ--STRAHKNVNVSDIKVGVCVFAFDILYLNGQLLIH 417

Query: 633 RELNVRRELLYDLFMEEPGYFQFATAIITNDLEEIQKFLDASVDQSCEGLIIKTLNRDAT 692
             LN+RRE L+D F E+ GYFQFATA+ +ND+ E+Q+FL AS+D  CEGL+IK+L  +AT
Sbjct: 418 ENLNIRREKLHDSFEEDLGYFQFATALTSNDIGELQEFLKASIDIGCEGLMIKSLYSNAT 477

Query: 693 YEPSKRSHNWLKLKKDYIDNIGDSLDLVPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQS 752
           YEP+KRS+NWLKLKKDY+DNIGDS+DLVPIA FHGRGKRTG +GA+LLACYD D EEFQS
Sbjct: 478 YEPAKRSNNWLKLKKDYMDNIGDSVDLVPIATFHGRGKRTGFFGAYLLACYDVDKEEFQS 537

Query: 753 ICKIGTGFSEAVLEERSASLRSKVIPEPKSYYRYGDTINPDVWFEPTEVWEVKAADLTIS 812
           ICKIGT FS+  L++ S+SL SKVI  PK YY+  + +NPDVW EPTEVWEVKAADLT+S
Sbjct: 538 ICKIGTEFSDVELQDLSSSLCSKVIATPKQYYQVDNDLNPDVWLEPTEVWEVKAADLTVS 597

Query: 813 PVHRAGVGIVDSDKGISLRFPRLVRVRKDKNPEEASSSE 845
           PV+R  +GIVD DKGISLRFPRLVR+RKDKNPEEA++S+
Sbjct: 598 PVYREAIGIVDPDKGISLRFPRLVRIRKDKNPEEATTSD 634

BLAST of Sgr023327 vs. TAIR 10
Match: AT1G66730.1 (DNA LIGASE 6 )

HSP 1 Score: 477.6 bits (1228), Expect = 2.4e-134
Identity = 271/690 (39.28%), Postives = 402/690 (58.26%), Query Frame = 0

Query: 196  ETKLRQDNGGSVGTKNVLAANKTCATSVQERTAKLKSCVPELKMKAKDFNSKNVACWKEG 255
            E K   +N G        A N    TS +E   K    +P  K + K+      ACW+EG
Sbjct: 715  EDKKASENSGH-------AVNICVQTSAEESVDKNYVSLPPEKYQPKEH-----ACWREG 774

Query: 256  ERVPFLFLCLGFDMVSEESSRITITDIVCNMLRTVMDTTPDDLVATVYLLANKIAPAHEG 315
            +  P++ L   F  V  E  +I    ++CNM R++   +P+D++  VYL  NKIA  HE 
Sbjct: 775  QPAPYIHLVRTFASVESEKGKIKAMSMLCNMFRSLFALSPEDVLPAVYLCTNKIAADHEN 834

Query: 316  LELGIGDASIIKALSEAFG------RTEAQELGDLGLVAKASRSSQSMMRKPDALTIAKV 375
            +EL IG + I  AL EA G      R     LGDLG VA+  R +Q ++  P  L +  V
Sbjct: 835  IELNIGGSLISSALEEACGISRSTVRDMYNSLGDLGDVAQLCRQTQKLLVPPPPLLVRDV 894

Query: 376  FDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPLYLIRLLQTKLRIGLAEQTLLAALG 435
            F T R I+ ++G  S   KKN I  L+ +  + E  +L+R L   LRIG   +T+L ALG
Sbjct: 895  FSTLRKISVQTGTGSTRLKKNLIVKLMRSCREKEIKFLVRTLARNLRIGAMLRTVLPALG 954

Query: 436  QAAV---YAEKHSTPPPN--IQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLSKT 495
            +A V   +   H+        +  LE  +  V + Y+ILP  D +VP+L+   +   + T
Sbjct: 955  RAIVMNSFWNDHNKELSESCFREKLEGVSAAVVEAYNILPSLDVVVPSLMDKDIEFSTST 1014

Query: 496  CNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGSVEIYSRNA 555
             +  PGIPI PMLAK  KGV E  N  Q+  FTCEYKYDG+RAQIH + +G+V I+SRN 
Sbjct: 1015 LSMVPGIPIKPMLAKIAKGVQEFFNLSQEKAFTCEYKYDGQRAQIHKLLDGTVCIFSRNG 1074

Query: 556  ERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDR-GKQKILPFQILSTRARKN----V 615
            +  T +FPD+V  + +   P+ ++F+LD E+VA DR    K++ FQ LSTR R +    +
Sbjct: 1075 DETTSRFPDLVDVIKQFSCPAAETFMLDAEVVATDRINGNKLMSFQELSTRERGSKDALI 1134

Query: 616  AVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFME-EPGYFQFATAIIT---- 675
                IKV+VCVF FD+++++G+ L+   L  RR  L ++F E  PGY ++A  I      
Sbjct: 1135 TTESIKVEVCVFVFDIMFVNGEQLLALPLRERRRRLKEVFPETRPGYLEYAKEITVGAEE 1194

Query: 676  ------NDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLKKDYIDNIGD 735
                  + L  I  FL+ +   SCEG+++K+L+ +A Y P+KRS +WLK+K+DY+D +GD
Sbjct: 1195 ASLNNHDTLSRINAFLEEAFQSSCEGIMVKSLDVNAGYCPTKRSDSWLKVKRDYVDGLGD 1254

Query: 736  SLDLVPIAAFHGRGKRTGVYGAFLLACYDNDNEEFQSICKIGTGFSEA-VLEERSASLRS 795
            +LDLVPI A++G G++ G Y  FL+AC++ + EEFQS+C++ +GFS+A  +E +      
Sbjct: 1255 TLDLVPIGAWYGNGRKAGWYSPFLMACFNPETEEFQSVCRVMSGFSDAFYIEMKEFYSED 1314

Query: 796  KVIPEPKSYYRYGDTINPDVWFEPTEVWEVKAADLTISPVHRAGVGIVDSDKGISLRFPR 854
            K++ +   YYR G+T  PD+WF    VWE++ AD T+SPVH A +G+V   +GIS+RFPR
Sbjct: 1315 KILAKKPPYYRTGET--PDMWFSAEVVWEIRGADFTVSPVHSASLGLVHPSRGISVRFPR 1374

BLAST of Sgr023327 vs. TAIR 10
Match: AT5G57160.1 (DNA ligase IV )

HSP 1 Score: 124.8 bits (312), Expect = 4.0e-28
Identity = 126/509 (24.75%), Postives = 221/509 (43.42%), Query Frame = 0

Query: 364 LTIAKVFDTFRLIAKESGKDSQEKKKNHIKSLLVAATDCEPL-YLIRLLQTKLRIGLAEQ 423
           LTI ++ D    +A    +     +K  + S L+  T+ + + ++IR++   L++G++E+
Sbjct: 125 LTIKELNDLLDRLASSENR----AEKTLVLSTLIQKTNAQEMKWVIRIILKDLKLGMSEK 184

Query: 424 TLLAALGQAAVYAEKHSTPPPNIQSPLEEAAKIVKQVYSILPVYDKLVPALLSGGVWNLS 483
                    +++ E H            +A  +      +  V +KL             
Sbjct: 185 ---------SIFQEFH-----------PDAEDLFNVTCDLKLVCEKLRDR------HQRH 244

Query: 484 KTCNFTPGIPIGPMLAKPTKGVSEILNKFQDLEFTCEYKYDGERAQIHYMENGS-VEIYS 543
           K  +   G  + P LA     V+    K    +   E K+DG+R QIH  +NG+ +  +S
Sbjct: 245 KRQDIEVGKAVRPQLAMRIGDVNAAWKKLHGKDVVAECKFDGDRIQIH--KNGTDIHYFS 304

Query: 544 RN---AERNTGKFPDVVITVSRVKKPSVKSFILDCELVAYDRGKQKILPF---QILSTRA 603
           RN            D++     V+   V   ILD E++ +D    +   F   Q ++  A
Sbjct: 305 RNFLDHSEYAHAMSDLI-----VQNILVDKCILDGEMLVWDTSLNRFAEFGSNQEIAKAA 364

Query: 604 RKNVAVSDIKVDVCVFAFDLLYLDGQPLIQRELNVRRELLYDLFMEEPGYFQF------- 663
           R+ +   D    +C  AFD+LY+    +I + L  R ELL  +     G  +        
Sbjct: 365 REGL---DSHKQLCYVAFDVLYVGDTSVIHQSLKERHELLKKVVKPLKGRLEVLVPEGGL 424

Query: 664 ---------ATAIITNDLEEIQKFLDASVDQSCEGLIIKTLNRDATYEPSKRSHNWLKLK 723
                    + +I+ +   ++++F   +V+   EG+++K L  ++ +EP  RS  W+KLK
Sbjct: 425 NVHRPSGEPSWSIVVHAAADVERFFKETVENRDEGIVLKDL--ESKWEPGDRSGKWMKLK 484

Query: 724 KDYIDNIGDSLDLVPIAAFHGRGKRTGVYGAFLLACYDNDN-----EEFQSICKIGTGFS 783
            +YI   G  LD++ I  ++G G+R G    FL+A  D          F S C++GTG S
Sbjct: 485 PEYI-RAGADLDVLIIGGYYGSGRRGGEVAQFLVALADRAEANVYPRRFMSFCRVGTGLS 544

Query: 784 E----AVLEERSASLRSKVIPE--PKSYYRYGD--TINPDVWFEPTEVWEVKAADLTISP 836
           +     V+ +     R    P+  P S+Y+  +     PDVW +  E   + +    I  
Sbjct: 545 DDELNTVVSKLKPYFRKNEHPKKAPPSFYQVTNHSKERPDVWIDSPEKSIILSITSDIRT 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900958.10.0e+0092.83DNA ligase 1 isoform X1 [Benincasa hispida][more]
XP_022140452.10.0e+0093.36DNA ligase 1-like [Momordica charantia][more]
TYK24176.10.0e+0091.56DNA ligase 1-like [Cucumis melo var. makuwa][more]
KAA0037310.10.0e+0091.43DNA ligase 1-like [Cucumis melo var. makuwa][more]
XP_004145818.10.0e+0091.43DNA ligase 1 [Cucumis sativus] >KGN46876.1 hypothetical protein Csa_021053 [Cucu... [more]
Match NameE-valueIdentityDescription
Q425721.1e-28866.41DNA ligase 1 OS=Arabidopsis thaliana OX=3702 GN=LIG1 PE=2 SV=2[more]
P518927.8e-17846.09DNA ligase 1 OS=Xenopus laevis OX=8355 GN=lig1 PE=2 SV=1[more]
Q869E11.0e-17749.45DNA ligase 1 OS=Dictyostelium discoideum OX=44689 GN=lig1 PE=3 SV=1[more]
P188586.6e-17750.49DNA ligase 1 OS=Homo sapiens OX=9606 GN=LIG1 PE=1 SV=1[more]
P379132.5e-17650.00DNA ligase 1 OS=Mus musculus OX=10090 GN=Lig1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1CGY40.0e+0093.36DNA ligase OS=Momordica charantia OX=3673 GN=LOC111011122 PE=3 SV=1[more]
A0A5D3DKM30.0e+0091.56DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1754G00060 PE... [more]
A0A5A7T1R10.0e+0091.43DNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold3G00220 PE=3 ... [more]
A0A0A0KGJ20.0e+0091.43DNA ligase OS=Cucumis sativus OX=3659 GN=Csa_6G148180 PE=3 SV=1[more]
A0A1S3CNQ90.0e+0091.30DNA ligase OS=Cucumis melo OX=3656 GN=LOC103503033 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G08130.17.7e-29066.41DNA ligase 1 [more]
AT1G49250.11.9e-23269.43ATP-dependent DNA ligase [more]
AT1G66730.12.4e-13439.28DNA LIGASE 6 [more]
AT5G57160.14.0e-2824.75DNA ligase IV [more]
InterPro
Analysis Name: InterPro Annotations of Monk fruit (Qingpiguo) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012310DNA ligase, ATP-dependent, centralPFAMPF01068DNA_ligase_A_Mcoord: 495..699
e-value: 8.4E-66
score: 221.5
IPR012310DNA ligase, ATP-dependent, centralPROSITEPS50160DNA_LIGASE_A3coord: 601..737
score: 44.509647
NoneNo IPR availableGENE3D2.40.50.140coord: 708..848
e-value: 2.0E-51
score: 175.9
NoneNo IPR availableGENE3D3.30.470.30DNA ligase/mRNA capping enzymecoord: 524..649
e-value: 1.4E-77
score: 261.8
NoneNo IPR availableGENE3D3.30.1490.70coord: 505..704
e-value: 1.4E-77
score: 261.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 153..183
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 132..190
NoneNo IPR availablePANTHERPTHR45674DNA LIGASE 1/3 FAMILY MEMBERcoord: 118..845
NoneNo IPR availablePANTHERPTHR45674:SF4DNA LIGASE 1coord: 118..845
NoneNo IPR availableCDDcd07900Adenylation_DNA_ligase_I_Eukcoord: 484..702
e-value: 5.66595E-144
score: 426.587
NoneNo IPR availableCDDcd07969OBF_DNA_ligase_Icoord: 707..844
e-value: 3.3894E-85
score: 268.961
NoneNo IPR availableSUPERFAMILY56091DNA ligase/mRNA capping enzyme, catalytic domaincoord: 487..705
IPR036599DNA ligase, ATP-dependent, N-terminal domain superfamilyGENE3D1.10.3260.10coord: 236..497
e-value: 1.2E-91
score: 309.1
IPR036599DNA ligase, ATP-dependent, N-terminal domain superfamilySUPERFAMILY117018ATP-dependent DNA ligase DNA-binding domaincoord: 241..486
IPR000977DNA ligase, ATP-dependentTIGRFAMTIGR00574TIGR00574coord: 317..844
e-value: 1.8E-147
score: 490.1
IPR012309DNA ligase, ATP-dependent, C-terminalPFAMPF04679DNA_ligase_A_Ccoord: 724..835
e-value: 3.6E-24
score: 85.2
IPR012308DNA ligase, ATP-dependent, N-terminalPFAMPF04675DNA_ligase_A_Ncoord: 259..428
e-value: 1.9E-39
score: 135.8
IPR016059DNA ligase, ATP-dependent, conserved sitePROSITEPS00697DNA_LIGASE_A1coord: 519..527
IPR016059DNA ligase, ATP-dependent, conserved sitePROSITEPS00333DNA_LIGASE_A2coord: 673..699
IPR012340Nucleic acid-binding, OB-foldSUPERFAMILY50249Nucleic acid-binding proteinscoord: 707..844

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Sgr023327.1Sgr023327.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071897 DNA biosynthetic process
biological_process GO:0006266 DNA ligation
biological_process GO:0006310 DNA recombination
biological_process GO:0006281 DNA repair
biological_process GO:0006273 lagging strand elongation
cellular_component GO:0005739 mitochondrion
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0003677 DNA binding
molecular_function GO:0003910 DNA ligase (ATP) activity
molecular_function GO:0003909 DNA ligase activity