Sgr023299 (gene) Monk fruit (Qingpiguo) v1

BLAST of Sgr023299 vs. NCBI nr
Match: RYQ97876.1 (hypothetical protein Ahy_B08g093951 [Arachis hypogaea])

HSP 1 Score: 188.3 bits (477), Expect = 9.0e-44
Identity = 112/200 (56.00%), Postives = 133/200 (66.50%), Query Frame = 0

Query: 1   MLVLNGRSVRGIIGSRASITNRVTLLESSWIPSARTWRPYSSAARALAIAAVLRRLFAAM 60
           MLVLNG SVRGIIGSRASITNRVTLL SSWIPSA+TW PYSSAARALAIAAV RR   A 
Sbjct: 1   MLVLNGLSVRGIIGSRASITNRVTLLGSSWIPSAKTWSPYSSAARALAIAAVSRRQLTAT 60

Query: 61  YSAAPAVSSTASRVTLRPNFVKPM-----------------------------------A 120
            SAAPAVSSTASR TLRP+FV+ +                                   A
Sbjct: 61  NSAAPAVSSTASRATLRPSFVRSLRVRDYAAEVLLSYAGESEEAVVDRELYLANDVEAVA 120

Query: 121 EQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFRVSSRN 166
           E++I++P+DR+A+ VFN + G++ +P+   L+ HLKLVAR  L +R+ FSGGG  V + N
Sbjct: 121 EEEIVVPVDRSAKGVFNREYGAVGNPELHCLERHLKLVARYGLTVRVSFSGGGLGVCAGN 180

BLAST of Sgr023299 vs. NCBI nr
Match: RDX67084.1 (hypothetical protein CR513_54080, partial [Mucuna pruriens])

HSP 1 Score: 124.4 bits (311), Expect = 1.6e-24
Identity = 67/144 (46.53%), Postives = 101/144 (70.14%), Query Frame = 0

Query: 81  VKPMAEQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFR 140
           V+ +AE+++++P+DRAAE VF+ ++GS+ DP+  RL+ HL+LVA N LA+RI F+GGGF 
Sbjct: 243 VEAVAEEEVVVPVDRAAEGVFHREDGSVGDPELHRLERHLELVAGNGLAVRIGFAGGGFG 302

Query: 141 VSSRNALVSHTKLRAVHRSRREVSNGERLRLRLGYTVGIGNIPNRNQIKVLNVRLMAIDR 200
           V + NALV HT+LRAVHR R EV + +R R R    +G   +P+RN++++ +   +A+D 
Sbjct: 303 VRAGNALVRHTELRAVHRRRCEVGDCQRFR-RKRLVLGFAKVPDRNELEIAHGGFVAVDG 362

Query: 201 HYMAGTTSAGRFIGRLLLRHDVAG 225
             + G  +A    G LLLR D+AG
Sbjct: 363 GDVVG-AAAVIVSGGLLLRDDLAG 384

BLAST of Sgr023299 vs. NCBI nr
Match: KCW87417.1 (hypothetical protein EUGRSUZ_B03894 [Eucalyptus grandis])

HSP 1 Score: 107.8 bits (268), Expect = 1.5e-19
Identity = 76/201 (37.81%), Postives = 113/201 (56.22%), Query Frame = 0

Query: 81  VKPMAEQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFR 140
           V+ +AE+++++P+DRAAE V + ++G++ DP+   L+  L+LVAR+RLA+RI F GG F 
Sbjct: 237 VEAVAEEEVVVPVDRAAEGVLHRQDGAVRDPELHGLEGDLELVARDRLALRIGFPGGCFG 296

Query: 141 VSSRNALVSHTKLRAVHRSRREVSNGERLRLRLG---YTVGIGNIPNRNQIKVLNVRLMA 200
           V S +ALV H +LR VHRSR +V +GERL  RLG   + + +    +  ++    V +  
Sbjct: 297 VGSGDALVGHAELRTVHRSRGQVGDGERLG-RLGGGRWELVVDVDGDEGEVDGGLVPVDG 356

Query: 201 IDRHYMAGTTSAGRFIGRLLLRHDVAGEDSTRALPIGTGEANAVLDAERFRSIGTLDGER 260
            D    A     G   G  +L  D+AGED   ALP+G   A+AV   +R   +G      
Sbjct: 357 GDAVGSAAVGVVGGGGGLAVLADDLAGEDGAGALPVGLAGADAVGVVDRVGPVGA----- 416

Query: 261 VGRQEAGKGTAAEKGIEGRRW 279
            GR   G G   + G + R W
Sbjct: 417 PGRVVGGGG---DPGEQRRAW 428

BLAST of Sgr023299 vs. NCBI nr
Match: KAF8039552.1 (hypothetical protein BT93_B1922 [Corymbia citriodora subsp. variegata])

HSP 1 Score: 105.9 bits (263), Expect = 5.9e-19
Identity = 73/187 (39.04%), Postives = 107/187 (57.22%), Query Frame = 0

Query: 81  VKPMAEQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFR 140
           V+ +AE+++++ +DRAAE V + ++G++SDP+   L+  L+LVAR+ LAIRI F G  F 
Sbjct: 304 VEAVAEEEVVVAVDRAAEGVLHRQDGAVSDPELHGLEGDLELVARDGLAIRIGFPGRCFG 363

Query: 141 VSSRNALVSHTKLRAVHRSRREVSNGERLRLRLGYTVG---IGNIPNRNQIKVLNVRLMA 200
           V S +ALV H +LR VHRSR +V +GERLR RLG   G   +G   +  ++ V +  LM 
Sbjct: 364 VGSGDALVGHAELRPVHRSRGQVRDGERLR-RLGDGRGELVVGVDWDEGKVDV-DGGLMP 423

Query: 201 IDRHYMAGTTS-------------AGRFIGRLLLRHDVAGEDSTRALPIGTGEANAVLDA 252
           +D     G  +              G   G  +L  D+AGED   ALP+G   A+AV   
Sbjct: 424 VDGGDAVGPAAVRVIGGGGGLAVLGGDLGGLAVLADDLAGEDGAGALPVGLARADAVGVV 483

BLAST of Sgr023299 vs. NCBI nr
Match: BAT90461.1 (hypothetical protein VIGAN_06171200 [Vigna angularis var. angularis])

HSP 1 Score: 95.5 bits (236), Expect = 7.9e-16
Identity = 50/90 (55.56%), Postives = 67/90 (74.44%), Query Frame = 0

Query: 81  VKPMAEQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFR 140
           V+ +AE+++++P+ RAAE VFN ++ +I DP+  RL+SHL+LVAR    +RIRFS  G R
Sbjct: 31  VEAVAEEEVVVPVYRAAEGVFNRQHRAIRDPELHRLESHLELVARYGFPVRIRFSRCGLR 90

Query: 141 VSSRNALVSHTKLRAVHRSRREVSNGERLR 171
           V S NALV H +LRAVHR R EV + ERLR
Sbjct: 91  VRSGNALVRHAQLRAVHRCRGEVGDRERLR 120

BLAST of Sgr023299 vs. ExPASy TrEMBL
Match: A0A444Y7Q4 (Uncharacterized protein OS=Arachis hypogaea OX=3818 GN=Ahy_B08g093951 PE=4 SV=1)

HSP 1 Score: 188.3 bits (477), Expect = 4.4e-44
Identity = 112/200 (56.00%), Postives = 133/200 (66.50%), Query Frame = 0

Query: 1   MLVLNGRSVRGIIGSRASITNRVTLLESSWIPSARTWRPYSSAARALAIAAVLRRLFAAM 60
           MLVLNG SVRGIIGSRASITNRVTLL SSWIPSA+TW PYSSAARALAIAAV RR   A 
Sbjct: 1   MLVLNGLSVRGIIGSRASITNRVTLLGSSWIPSAKTWSPYSSAARALAIAAVSRRQLTAT 60

Query: 61  YSAAPAVSSTASRVTLRPNFVKPM-----------------------------------A 120
            SAAPAVSSTASR TLRP+FV+ +                                   A
Sbjct: 61  NSAAPAVSSTASRATLRPSFVRSLRVRDYAAEVLLSYAGESEEAVVDRELYLANDVEAVA 120

Query: 121 EQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFRVSSRN 166
           E++I++P+DR+A+ VFN + G++ +P+   L+ HLKLVAR  L +R+ FSGGG  V + N
Sbjct: 121 EEEIVVPVDRSAKGVFNREYGAVGNPELHCLERHLKLVARYGLTVRVSFSGGGLGVCAGN 180

BLAST of Sgr023299 vs. ExPASy TrEMBL
Match: A0A0A0LSY2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G148480 PE=4 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 3.1e-26
Identity = 78/187 (41.71%), Postives = 118/187 (63.10%), Query Frame = 0

Query: 81  VKPMAEQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFR 140
           +K M E++I+IP+DR AE VFN +NG+I DP+F+RLK   +L+AR+ +A+R+ F+GGGF 
Sbjct: 1   MKLMTEEEIVIPVDRTAEGVFNRENGAIGDPEFNRLKGDFELIARDGVAVRVSFTGGGFG 60

Query: 141 VSSRNALVSHTKLRAVHRSRREVSNGERLRLRLGYTVGIGNIPNRNQIKVLNVRLMAIDR 200
           V + N+LVS  ++ AVHR   EV +G+   L      G+G + + ++++VL+  ++AID 
Sbjct: 61  VCAGNSLVSDAEVGAVHRRGGEVGDGKGFGLGF-ERGGVGEVLDGDKVEVLDGGVVAIDG 120

Query: 201 HYMAGTTSAGRFIGRLLLRHD---VAGEDSTRALPIGTGEANAVLDAERFRSIGTLDGER 260
             + G     R   R++LR +   +AGED T ALP+GTG A+ V   +   SI  LD E 
Sbjct: 121 GDVIGRRRRRRRRRRVILREESLGLAGEDGTGALPVGTGGADGVGVGDGVVSIDGLDTEA 180

Query: 261 VGRQEAG 265
            GR   G
Sbjct: 181 DGRCGGG 186

BLAST of Sgr023299 vs. ExPASy TrEMBL
Match: A0A371EM88 (Uncharacterized protein (Fragment) OS=Mucuna pruriens OX=157652 GN=CR513_54080 PE=4 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 7.7e-25
Identity = 67/144 (46.53%), Postives = 101/144 (70.14%), Query Frame = 0

Query: 81  VKPMAEQKIIIPMDRAAERVFNGKNGSISDPKFDRLKSHLKLVARNRLAIRIRFSGGGFR 140
           V+ +AE+++++P+DRAAE VF+ ++GS+ DP+  RL+ HL+LVA N LA+RI F+GGGF 
Sbjct: 243 VEAVAEEEVVVPVDRAAEGVFHREDGSVGDPELHRLERHLELVAGNGLAVRIGFAGGGFG 302

Query: 141 VSSRNALVSHTKLRAVHRSRREVSNGERLRLRLGYTVGIGNIPNRNQIKVLNVRLMAIDR 200
           V + NALV HT+LRAVHR R EV + +R R R    +G   +P+RN++++ +   +A+D 
Sbjct: 303 VRAGNALVRHTELRAVHRRRCEVGDCQRFR-RKRLVLGFAKVPDRNELEIAHGGFVAVDG 362

Query: 201 HYMAGTTSAGRFIGRLLLRHDVAG 225
             + G  +A    G LLLR D+AG
Sbjct: 363 GDVVG-AAAVIVSGGLLLRDDLAG 384

BLAST of Sgr023299 vs. ExPASy TrEMBL
Match: A0A0E0GFT0 (Uncharacterized protein OS=Oryza nivara OX=4536 PE=4 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 6.1e-22
Identity = 102/295 (34.58%), Postives = 139/295 (47.12%), Query Frame = 0

Query: 1   MLVLNGRSVRGIIGSRASITNRVTLLESSWIPSARTWRPYSSAARALAIAAVLRRLFAAM 60
           MLVLNGRSVRG++GSRASITNRVT   SSW+PSA TW PYSSAAR  A+           
Sbjct: 57  MLVLNGRSVRGMMGSRASITNRVTFPASSWMPSASTWSPYSSAAREDAM----------- 116

Query: 61  YSAAPAVSSTASRVTLRPNFVKPMAEQKIIIPMDRAAE---------RVFNGKNGSISDP 120
             AAP               V+ +AE+++++ +D AAE          V +G++G++ DP
Sbjct: 117 --AAPRGGDVLGD-------VEAVAEEEVVVAVDAAAEGVLDGEDGAGVLDGEDGAVGDP 176

Query: 121 KFDRLKSHLKLVARNRLAIRIRFSGGGFRVSSRNALVSHTKLRAVHRSRREVSNGERLRL 180
           + D L+ HL+LVA + +A  +        V  R+ LV   +L AVH    EV +GER R 
Sbjct: 177 ELDGLEGHLELVAGDGVAGGVGLRRRRLAVCPRDTLVGDAQLAAVHGRGGEVGDGERAR- 236

Query: 181 RLGYTVGIGNIPNRNQIKVLNVRLMAIDRHYMAGTTSAGRFIGRLLLRHDVAGEDSTRAL 240
                VG G      QI+V         R  + G       +   +   D A +     +
Sbjct: 237 ----EVGDG-----EQIQVER-------RRGVVGEGGLDATVAVAVHPRDSA-DGGAGGV 296

Query: 241 PIGTGEANAVLDAERFRSIGTLDGERVGRQEAGKGTAAEKGIEGRRWRWRRRVES 287
           P+G GE      A+  R  G + G R GR       +  +    R W WR R  S
Sbjct: 297 PVGAGERGGARRADG-RGSGDVGGARRGRGFHPPAVSGGEAGAARGW-WRERAAS 311

BLAST of Sgr023299 vs. ExPASy TrEMBL
Match: A0A7C9D5Y8 (Uncharacterized protein OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 109.8 bits (273), Expect = 2.0e-20
Identity = 65/81 (80.25%), Postives = 67/81 (82.72%), Query Frame = 0

Query: 1   MLVLNGRSVRGIIGSRASITNRVTLLESSWIPSARTWRPYSSAARALAIAAVLRRLFAAM 60
           MLVLNGRSVRGIIGSRASITNRVTLLESS IPSA T  PYSSAARA AIAAV  +   A 
Sbjct: 61  MLVLNGRSVRGIIGSRASITNRVTLLESSCIPSANTCSPYSSAARAEAIAAVSLKRLVAT 120

Query: 61  YSAAPAVSSTASRVTLRPNFV 82
           YSAAPAVSS ASRVT +PN V
Sbjct: 121 YSAAPAVSSKASRVTFKPNLV 141